LOC764024 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC764024
Entrez Gene ID	764024
Full Name	sacsin-like
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011676623.1	XP_011674925.1	sacsin-like

Pathways from BioSystems
Gene Ontology

The following LOC764024 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC764024 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSd29817	XM_011676623.1 Latest version!	Strongylocentrotus purpuratus sacsin-like (LOC764024), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC764024 ( XM_011676623.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011674925.1 Strongylocentrotus purpuratus sacsin-like (LOC764024), mRNA.

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CloneID	OSd29817
Clone ID Related Accession (Same CDS sequence)	XM_011676623.1
Accession Version	XM_011676623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	sacsin-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011968495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 100% of CDS bases ##RefSeq-Attributes-END##

ATGCCGCTTT TCACAGACCA GGACTTTAAG AATATTCTCC GTCTTGGAGG GCGTACCAAG 
GAAAAGGACG CTGAAAAGAT CGGGAAGTTT GGGACCGGTT TCAACTCGGT GTACCGCATA 
ACTGATGTTC CAAGTTTGGT TAGTAGGGAC TACATGCAGT TTTTCGACCC TCATACCACA 
CACCTTGGAA ACGTTCTCCC GAACAAGAGT CAACCCGGTG TTCGTTTCAG ATTAAATAGC 
ACCAAAACAC ACCGGAGATT TCAAGATCAG TTCAAGCCAT TCAACGGAGT GTTTGGGCTC 
GATTTCAGTG GAGGAAATGA ACCATTCAAC TACAACGGAA CACTCTTTAG ACTGCCCCTC 
CGTTCTAAAG AAGCTGCACA GAAAAGCCAA ATATGCAAGG AGAGCTACGA TGAGGAAAAA 
CTGACCAACC TGATGCAGAT AATGTGGGAA TCATCACAAA ACCTCCTGGT TTTCACCAAA 
AAGGTAAAGA AGGTTGCCTT GTTCTACCTT TCTGAGAATT CTAGCGATCC TACCAGGGCT 
GAGGAATTAC TCACTATACA GAAGACCATT CACTCTCCTA GGTTTAAGGA TGACGGCACC 
GTCGTGTCAA CTGAATCATT CCGCTGCCAA CTAACCTCAA GGGGAGCTGA AGTATTGCCA 
CACACCAATG AAATAGGCAG TCAGGAGCAT ACGAGTTCTT TCAACACCTT CAAAGTGGTA 
TGCAAGACTT CAAAACAAGC ATTAGTTGTC GCACAATCTC AGGAAGGAAA ATCACATGGG 
CTTTCTCCAG CGGCTGCCGT AGCTATCACA TTGAATGTAA GAGATTTGCA GAAAATCGAG 
GGGAAGATCT ACTGCGGTCT ACCTTTGTCA GTTCCAAGCC TGCTTCCATT TCACATGGAT 
GGTACGTTCG CCATTAACGA AGATCGAAGA AGTCTACAAT CACCGACACC ACATGGGATG 
ACTGTTTTTG AAAGATGGAA TCATATATTG CTCTCTGACG TGATTTGCCG AGCATATGTC 
TCTCTTCTAT CAGACAAAGA GTTCATTCAA CAGATCAAGA ATACATACGG GACAGATGTG 
TCGCCAGAGT TGTTGTGGCC TGATGTTGAT AAAGTACCTG AGAATTCAGT GTGTTCAGGG 
CTGTTTAATG CTTTCTACAA AACGATAATC TGCGGATTGG ATGGCAAACA ACCAGCAGTT 
TTCTGGACAA ATGACGAACC TCGTCAGTTT CACAATGTAG TTTTTCTCGG CGAGGAACTG 
CAAACGATGA AAGGAATCAA GGACGACTGT TTACAGATTT TGACAACGTA TCTGAAGGAG 
TACTGCCACA GTTGCAGTAT GGTGGAAGTG ATAGAGCTAT CAAAGAAGAC GTGGCAAGCA 
CTCGAGAGGG CAGGAGTACG TGATATGGTA GAGACTAGAA CATACAATCT GAACAGGTTG 
TTTGATGAAG TGTTCCTGCC ATATTTAGAT AAAGTGCCAC AGGATCTGAG AGATCACCTC 
ATTCTCTTTG CGCTTGAAAT GGACGGTAAC CTGCATCAAA AACTTCGAGA TGTGAAATGC 
ATCACCGTCT TAGGAGGAGC CGAACTAAAG ACATCCTGCG CCCTCATGCA TCAAGATGAC 
CCTACACTCC GTGAATTGTT TGAGCTTCAG GGAGTATTTC CAGGTGCACC ATTTGATTCA 
GCTCATCTGA CGCCGGTATT CAACCGCCTT GGTATGCGAG GACCGAATGA AGTTACTCCA 
GTTGAGATGG TTGCATGTGT TGATCTTGTT GAGAAAACTC AGTGCCCCAA GAAGGCCAGA 
GCTTTACTCG ATCTCATCGG AAAGAACCCT GGTCATTTTG ACCGTTCACT GGCCGACGGC 
AGCACTGTAA GAGATTTCAT GATGAACAAA CGATCATTCA CACTGCTTGG AATAACATGC 
CACTCTCCAG TGATCTCGGA CGTCATAGCA CAAATAGAAC ATTGTGTGAA GTATGCCACA 
TACAGAACAG AACAAGATGT AACAGACATA ATACCGATGC TCAAGGAGAT CTATGCATTT 
CTGAGGAAAG AATGGCAACG TGATTCCAAC GTCAGAAACT ATATATTGGA TCGAGTAAAG 
TGTTGTGTGT GGAATGGCAA CGGCTTTTCG GAACCAAGCA AAATGTGTAC AGGTAATCTC 
CCGTTCGATC TAGCTCCTTA CATGGCATCT ATTACCCAGG CTACTTGTGC GTTTAAAGAT 
TTGTTTGAGG CGCTTGGGGT CAAAAGGAAC ATCGACACTA ATTCGGAAGC TCTGGTTGAA 
ATACTACATA AAATCAAGAG CAAATGTGAC GTCCTAGCAG CAAATGTAAT CTTCCAACAA 
AAACAGCGCC AACTTGTACT GCAGATCCTT CACCATCTAC ATGCCACAGG AGAGCTTTCA 
AAAGAAGCTC GGGTACGCTT ACTTGTTCCA TCGCAAAGAG GAAAATGTCA TCTCATTCCA 
GTTGATGAGA GCGCATACGT GGACAGAGAC TGGTTGAGAC AAAGCACCGA CAATGAGGAG 
ATAGATGACG ATGAAGACTT TACTTTGATT CATTCAGATA TCTCAATGGA ACTTGCAATA 
TTCCTCCACG TACGACCTGT CAGTCGACTT CTCCTAGATA CAGAAGAATT GCAGGGTTTC 
ACACCAGCTG GGCAGCATGA ACCCCTGACT ATGCGCTTGT GGAATATTCT CAAGAACAAC 
TATGTGGACA CCGCCATCGT TAGTGAAATG ATACAGAATG CGGAAGATGC AGGAGCTCAG 
GAAGTTCGCT TTCTCATTGA CATGAGAAGA AACGAGAAAG AAACCAAAAG ATTATTCGAT 
CCGGGAATGA AGTCTTGTCA GGGACCAGCC CTTTGGGTAT ACAACGATGC CGTATTCACA 
GACCAAGACT TTGAAAACAT TCTCTGTCTT GGAGGGCGTA CCAAGGAAAA GGACGCTGAA 
AAGATCGGGA AGTTTGGGAC CGGTTTCAAC TCGGTGTACC GCATAACTGA TGTTCCAAGT 
TTGGTTAGTA GGAACTACAT GCAGTGTTTC GACCCTCATA CCACACACCT TGGAAACGTT 
CTCCCGAACA AGAGTCAACC CGGTGTACGT CTCAGATTGA ATAGCTCCAA ACAACTCCGG 
AGATTCCCAG ATCAGTTCAA GCCATTTAAT GGAGTGTTCG GGCTCGATTT CAGTGGAGGA 
AATGAGCCAT TCAACTACAA CGGAACACTC TTTAGACTGC CCCTCCGTTC CAAAGTAGCT 
GCACAGAAAA GCCAAATATG CAAGGAGAGC TACGATGGGG AGAAAATGAT CAACCTGATG 
CAGAAAATGT GGGAATCATC ACAAAACCTC CTGGTTTTCA CAAAACAGGT AAAGAAGGTT 
TCCCTGTTCT ACCTTTCTGA GAATTCTAGC GATCCTACCA GTGCTGAGGA GTTACTCACC 
ATACAGAAGA CCATGCACTC TCCTAGGGTG AAGGATGACG ATACCATTGT GTCAACTGAA 
TCATTCCGCT GCACAATGAC TTCAAGGGGA AATGAAGTGA TGTCACAGAA ACCAGAAGTT 
AGTGGAGACC TGGACACTGT CAAAGTGATA TGCAAGACTT CAGAACAGGC AGCATCTCTT 
GCAAAATCTC CAGATGGCAG GGAGTGTGGA CTTTCACCAG AAGGTGCGGT TGCCTTTCCG 
TTTCATAGAC CACCAATGTC TGCATTTGAG AAGAAGATCT ACTGCTTCCT TCCCCTCTCA 
ATTAACAGTA TGCTCCCAGT GCATATCAAC GGATCTTTTG CTGTAAAAGA GGACCGGCGG 
AGTCTTCACA TGCCAGTGCC CGATGGAAGG TTGATATCTG AGAAATGGAA TCACATTATT 
CTGGCAGATG TGATATGCAG AGCGTACGTT TCACTCTTGT CTGACAGTGA GTTCATTCAA 
CAAGTGAAGG ACACATACGG CTCGGATATG TCTCCTGAGG AGTTGTGGCC AGATATTGAT 
GACGTGCCAA ATAATTCAGA ATGTTCAGCA CTGTTTGATG CTTTCTACAA GGCAATGGTA 
CATGGGTTGG ATGGCCGTGA ACCATCATTG TTCTGGAAAG ATGGCAAATC GCATCAGTTC 
ACGAATGTTG TGTTTCTTGG TGAAGAAGTT CAAAAGGAGA CATCTGTCAG TGATGTGGTA 
ACAAAGGTAC TAAGCGACCA TCTCCCCTCA ATGGAAGTGA TGTCATTGAC CGAGAAAACT 
TGGAGAGCCC TAAGAAGAGT ACAGCTGATA AATGAAGTTC TCAAGAGGAC CTATGATTTC 
AACAGGTTCT TCGATGAAGT TTTTCTACCA CAAATCAGTC AGGTGCCGGA CAACATCAGA 
AACCAACTGG TTCTGTTTGC ACTCAATAAC AGTGAAGACA CACTGCAGCA GAAACTAAAA 
GATGTGGAAT GCATACCAGT ACAGGGAGGA ACTGAACTGC GGAAACCTTG TGATCTAGTA 
CACCCGAAAG GCAAAGCTGC GCCTTTGTAC TCGGAGTCTG ACAAGGTTTT CCCTCTGGGA 
GAAAAATTCA ATGCCACCGA CACATTGGCG TCACTTAGTA AGCTAGGAAT GGTGAATGAC 
TATCTTCATG AGGATATTTT CGTCGAGCGA TGCGAGACTG TTCACATTCT GCATTCAAGC 
TGCCGGGAAG TAGCTCAAAC GAGATGCCAT GCTGTACTGA ATTATCTGTC GGAGATGTTG 
AGGAGTGATT GGAATGAGAA AAAAACCCTC AAAGAACGGC TTTCGAGTAT CCCATTCTTG 
CCTATTTTGT GTGAGAAAAA TAGAGACCAA GCTCTACCGT GGGTGGAAAC AGACTCTCTT 
GCTTATGCAT CTGCGAATGA TGTGTACTCT CATTCTAAAA CATATTTGGT AAGCAGCACG 
TCAAACGTTG TGGATGAATC AGAAGAGTGG AAGCTTCCCT TGACAGTCGA GCATTTTCTT 
GGTCTTGTAG ATAAATATCC CTCTGTCCAA GATGTACTCA GGCAGTTTTC AAGCTTTCGT 
AGTCACTACG AAACGAATAA TGAATGCATT TCTGGTCATC AGTTACAAAT GATATGCAAG 
GCGGTCTACG AGCGTGTTGA AATTGCTTGT GAAGACAATA GTCCTGACAT CTCCAACGAA 
GATCTTCAGA ATCAATCATT CTTCCTTGTA GATCGCAAGT TTGTAGTGGC CTCTATGGTT 
GTCGATAGAG GCAGAGATCT TCCACCGTAT CTATACAAAG CTCCCCAGTT CCTGAAACAA 
TATCCAATTC TTCTGGAACG TGCAGTATTG AAGAGGGAGT TCGATGCAGG AGACTATCAA 
TGTGTTCTCC AATCTATATA TGAGGAAAGC AATGGGAAAC CTTTAGACGA CGAGGTCTTG 
GAGGTTGCTA TTTTAGCTGC CGATCGACTA GCCAGTTTCC TCAAAGATGA TGATGATGGG 
AGACGGTCTC CAAATGCATT TCTCCCAGAT GAGCACAAGT GCATGAGGCC TGTATCAGAT 
CTCTGCTACA ATGACATTGA TTGGTTTGAA TACGACGAAA TCGAACTACA TAAGTGTCAC 
ATGAGAATTA GCTTGATGCT TGCAAAAAAG CTGAAAGTGA AGGATGTTCG ACAAATGATG 
CTCCGGCCAT ACGAAATGGA TTTCTCAGGG GAAGAGTTTG GCCAACATGA GAAACTTACA 
ACCCGTATCA AGCGGATTCT CGATTCGTAC CCGTCTGATG AAACAATTCT GAAAGAACTT 
CTTCAGAATG CAGACGATGC TGGAGCAACT GAAATCCACT TTGTCTACGA TCCTCGCTCA 
CATAGTGAAA ATAAACTTCT AGCAGATAGC ATGAAAGCTT TGCAGGGTCC AGCTCTTTGT 
GTCTTCAACA ACCAATCTTT TACAGATGAA GATATTCGTG GGATACAGAA TCTTGGAAAA 
GGTAGCAAAG CTGATCATAT GGAAAAGATA GGCCGTTATG GGGTCGGTTT CAATACGGTG 
TATCAACTTA CGGATTGCCC AAGTTTCATC TCCAAAGGTG AACGATTATG CATGTTTGAC 
CCTCTGCTGA AGTATATTCC TGGCGCAACA TCGACTAAGC CGGGCCGTCA GATAAGAGTT 
AATGACAGTG TACGTTCTTC GTATCCAGAT ACCTTTCAAT GTTACTTGGA AGACATTCTT 
GCAGATGGAA AGAAGGACTA TACTGTTTTT CGTTTTCCAT TGCGGGAGGA AGCTTCAACA 
CTTAGCAAAA ACGTATGGCG AGCTGAAAAG ATTCGGAAAT TACTACAAGA CTTTGAGAAT 
GCTGCATTCG AAAGTCTTTT GTTTCTGAAA AACATCACTA AAATCAGCAT CTCAGAAGTA 
ACAAGCTCAG GAGCGATTGG CTCTACTCAT GCTATCACAG CAAAGATAAC CGAAGTGGAA 
GGTGAAAAGA AAAATGATTT CCTGCGCAAA GTTCAAGATA TCAGTAGAGC AGCAAAGGAT 
GACGGTACCT GGCAAACCGA ACCAGCAGAA GTCATATACC AGATGAGATT GAAAGGGAAA 
GGAAGAGATG AGGATTGGGT AATATGTCAG CAAGTAGGAA GTACAGACAC GGCTGAAGAA 
AACATAATGC GAAAACGTAA ACTACTTCCA GTGGGGGCTG TTGCTGCCCG TCTGACAGGT 
AAAAAAGAGG AACTCAAAGG AACGGCTTTT TGCTTCTTAC CCCTTCCCAT TGCAACGGGA 
CTTCCAGTTC ACGTACACGG ATACTTCGCC TTAGACCATG AGAGTAGAAG ACACTTGTGG 
GAAGGTAGCG AAACAGATGA ACACACAAAG TGGAACTTTC ACCTCATCCA GAAAGTTATT 
GCACCGGCTT ATGTGAAGTT GCTGTTGTGG ATACAGGGAT TCTTTGTCAG GAAATATGAA 
TGGTCAGGAT CGAAGTCATG TCAACAAAAA GCTCACACTT TGAAACTCCT TCAGAAATAC 
CATACCTTCT TTCCTAACAT CGGCGAGAAA GGGTCTTACT GGAAAACACT TGAAGCCACT 
GTATACGAAA TTGTTGCCTC GGAAGCCCTG ATGCTATTTC CTTCTCTATA CAGAAAAGAT 
GATGGCAACT GGGACTTTTT TCTAACATGG AAGACCCCAG TCGTGCACAT GCAGCCTCCA 
GACGAATCAA AGGTGGGATA TTTCAACACA CTGCCAAACC AAATGAGTGA TCACTTCACA 
TTCCCTACAA GTAGCTCCTT GAAGGCTGAG ATGCAAGAAA ATGTGAAAAA AATGAAGGAG 
ACACTGATCG AAATCACATT TCCATTACTA GAGTCTCCGA CATGGTTAAT GCACAGGCTC 
CGTAATGCAT GCAAGCATCT CAATCAGGAA ACCATGAACC ATGAATCAGG AAACTATAAA 
GTTCTTGAAG TTCTACCTTC AAATGTGGTG TCTTTCCTTC GCGGAGACAA CCCTCTTCAG 
AGGCAATTAC CAATGCCACT TTCAGAATCA AGGATCCACG ACGAGGGGAG ACTGAATGGA 
CTCATAGATT ACTGCGAGAA GGGAACAAAA TCTAACTTCA AAGAAAGTCT CCACGGATTA 
CCATTGCTCT TCACTCAGGA TGGGAACTTG AGGAGGTTAT CACGTGATGA GCGAGTCTTT 
GCATCAGAAT TCTGCGATCT TTTCAAGCCT CAGAGTCATA GGTTTTTACA TATCGACTTT 
CTAGACACTC TACCACGAAA TGCCGATGTC TTTCGTGAGC TTGATGTGAG TGCAGTATCT 
GCGCTTCTTG ATGATTGCAA ATCTTTTCTA AAGTGTGCTT CCGATGAAAT AATAACGCTT 
CGGAACTACC TTCCATCTGA ATGGTTTGAA CGTTTGTGGA GTCTTCTTGA ATCCTTATGC 
ACAGAAACGA ACAAGTTGAC TGATGTTGCA CACGATATCA GAGTTGAACT TGGCCGTTGG 
GCGATATTAC CTGTCACAGA AAGAAAAACT GAAGGTCGTC CGAGAATTAG GCTAGTGGCA 
CCTATCAGTT TATCCTCTAC CATAATGGTC CAACCAACCA AGTTGGAAAA GAGTTGCTGG 
CCAATCTACT CAGCACTTGA ATCACTGAAC GTCTACAAGG TCGAAGAATC CATTCTTCAC 
GAAGACAAGA ATAGCTTAAT ACATCATCTC GTCACACACA AGGGCGATAC CACGAGGGTT 
TTAGATGCCT TGATGTACTG GGTTGATAGA GACGTCAAGA GATTCAGAAA CCTCGAAGCT 
GATCAACATG AAGAAATCCT GAGATTCTTC AGTAACTGTG AAAGCGAAGC TGATCTCAAA 
AGGTTGAAGA GCCTGCCTTG CTTCAAATCG ATTGCAGGTG ACTTTGTCAG TATCAGAGGT 
TTTGACACAG TTCTTGTCCT CCCAACAAGT ACAGAAATCA CATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011674925.1
CDS	1..8328
Translation	MPLFTDQDFKNILRLGGRTKEKDAEKIGKFGTGFNSVYRITDVPSLVSRDYMQFFDPHTTHLGNVLPNKSQPGVRFRLNSTKTHRRFQDQFKPFNGVFGLDFSGGNEPFNYNGTLFRLPLRSKEAAQKSQICKESYDEEKLTNLMQIMWESSQNLLVFTKKVKKVALFYLSENSSDPTRAEELLTIQKTIHSPRFKDDGTVVSTESFRCQLTSRGAEVLPHTNEIGSQEHTSSFNTFKVVCKTSKQALVVAQSQEGKSHGLSPAAAVAITLNVRDLQKIEGKIYCGLPLSVPSLLPFHMDGTFAINEDRRSLQSPTPHGMTVFERWNHILLSDVICRAYVSLLSDKEFIQQIKNTYGTDVSPELLWPDVDKVPENSVCSGLFNAFYKTIICGLDGKQPAVFWTNDEPRQFHNVVFLGEELQTMKGIKDDCLQILTTYLKEYCHSCSMVEVIELSKKTWQALERAGVRDMVETRTYNLNRLFDEVFLPYLDKVPQDLRDHLILFALEMDGNLHQKLRDVKCITVLGGAELKTSCALMHQDDPTLRELFELQGVFPGAPFDSAHLTPVFNRLGMRGPNEVTPVEMVACVDLVEKTQCPKKARALLDLIGKNPGHFDRSLADGSTVRDFMMNKRSFTLLGITCHSPVISDVIAQIEHCVKYATYRTEQDVTDIIPMLKEIYAFLRKEWQRDSNVRNYILDRVKCCVWNGNGFSEPSKMCTGNLPFDLAPYMASITQATCAFKDLFEALGVKRNIDTNSEALVEILHKIKSKCDVLAANVIFQQKQRQLVLQILHHLHATGELSKEARVRLLVPSQRGKCHLIPVDESAYVDRDWLRQSTDNEEIDDDEDFTLIHSDISMELAIFLHVRPVSRLLLDTEELQGFTPAGQHEPLTMRLWNILKNNYVDTAIVSEMIQNAEDAGAQEVRFLIDMRRNEKETKRLFDPGMKSCQGPALWVYNDAVFTDQDFENILCLGGRTKEKDAEKIGKFGTGFNSVYRITDVPSLVSRNYMQCFDPHTTHLGNVLPNKSQPGVRLRLNSSKQLRRFPDQFKPFNGVFGLDFSGGNEPFNYNGTLFRLPLRSKVAAQKSQICKESYDGEKMINLMQKMWESSQNLLVFTKQVKKVSLFYLSENSSDPTSAEELLTIQKTMHSPRVKDDDTIVSTESFRCTMTSRGNEVMSQKPEVSGDLDTVKVICKTSEQAASLAKSPDGRECGLSPEGAVAFPFHRPPMSAFEKKIYCFLPLSINSMLPVHINGSFAVKEDRRSLHMPVPDGRLISEKWNHIILADVICRAYVSLLSDSEFIQQVKDTYGSDMSPEELWPDIDDVPNNSECSALFDAFYKAMVHGLDGREPSLFWKDGKSHQFTNVVFLGEEVQKETSVSDVVTKVLSDHLPSMEVMSLTEKTWRALRRVQLINEVLKRTYDFNRFFDEVFLPQISQVPDNIRNQLVLFALNNSEDTLQQKLKDVECIPVQGGTELRKPCDLVHPKGKAAPLYSESDKVFPLGEKFNATDTLASLSKLGMVNDYLHEDIFVERCETVHILHSSCREVAQTRCHAVLNYLSEMLRSDWNEKKTLKERLSSIPFLPILCEKNRDQALPWVETDSLAYASANDVYSHSKTYLVSSTSNVVDESEEWKLPLTVEHFLGLVDKYPSVQDVLRQFSSFRSHYETNNECISGHQLQMICKAVYERVEIACEDNSPDISNEDLQNQSFFLVDRKFVVASMVVDRGRDLPPYLYKAPQFLKQYPILLERAVLKREFDAGDYQCVLQSIYEESNGKPLDDEVLEVAILAADRLASFLKDDDDGRRSPNAFLPDEHKCMRPVSDLCYNDIDWFEYDEIELHKCHMRISLMLAKKLKVKDVRQMMLRPYEMDFSGEEFGQHEKLTTRIKRILDSYPSDETILKELLQNADDAGATEIHFVYDPRSHSENKLLADSMKALQGPALCVFNNQSFTDEDIRGIQNLGKGSKADHMEKIGRYGVGFNTVYQLTDCPSFISKGERLCMFDPLLKYIPGATSTKPGRQIRVNDSVRSSYPDTFQCYLEDILADGKKDYTVFRFPLREEASTLSKNVWRAEKIRKLLQDFENAAFESLLFLKNITKISISEVTSSGAIGSTHAITAKITEVEGEKKNDFLRKVQDISRAAKDDGTWQTEPAEVIYQMRLKGKGRDEDWVICQQVGSTDTAEENIMRKRKLLPVGAVAARLTGKKEELKGTAFCFLPLPIATGLPVHVHGYFALDHESRRHLWEGSETDEHTKWNFHLIQKVIAPAYVKLLLWIQGFFVRKYEWSGSKSCQQKAHTLKLLQKYHTFFPNIGEKGSYWKTLEATVYEIVASEALMLFPSLYRKDDGNWDFFLTWKTPVVHMQPPDESKVGYFNTLPNQMSDHFTFPTSSSLKAEMQENVKKMKETLIEITFPLLESPTWLMHRLRNACKHLNQETMNHESGNYKVLEVLPSNVVSFLRGDNPLQRQLPMPLSESRIHDEGRLNGLIDYCEKGTKSNFKESLHGLPLLFTQDGNLRRLSRDERVFASEFCDLFKPQSHRFLHIDFLDTLPRNADVFRELDVSAVSALLDDCKSFLKCASDEIITLRNYLPSEWFERLWSLLESLCTETNKLTDVAHDIRVELGRWAILPVTERKTEGRPRIRLVAPISLSSTIMVQPTKLEKSCWPIYSALESLNVYKVEESILHEDKNSLIHHLVTHKGDTTRVLDALMYWVDRDVKRFRNLEADQHEEILRFFSNCESEADLKRLKSLPCFKSIAGDFVSIRGFDTVLVLPTSTEITF

Target ORF information:

RefSeq Version	XM_011676623.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus sacsin-like (LOC764024), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011676623.1

ORF Insert Sequence:

ATGCCGCTTT TCACAGACCA GGACTTTAAG AATATTCTCC GTCTTGGAGG GCGTACCAAG 
GAAAAGGACG CTGAAAAGAT CGGGAAGTTT GGGACCGGTT TCAACTCGGT GTACCGCATA 
ACTGATGTTC CAAGTTTGGT TAGTAGGGAC TACATGCAGT TTTTCGACCC TCATACCACA 
CACCTTGGAA ACGTTCTCCC GAACAAGAGT CAACCCGGTG TTCGTTTCAG ATTAAATAGC 
ACCAAAACAC ACCGGAGATT TCAAGATCAG TTCAAGCCAT TCAACGGAGT GTTTGGGCTC 
GATTTCAGTG GAGGAAATGA ACCATTCAAC TACAACGGAA CACTCTTTAG ACTGCCCCTC 
CGTTCTAAAG AAGCTGCACA GAAAAGCCAA ATATGCAAGG AGAGCTACGA TGAGGAAAAA 
CTGACCAACC TGATGCAGAT AATGTGGGAA TCATCACAAA ACCTCCTGGT TTTCACCAAA 
AAGGTAAAGA AGGTTGCCTT GTTCTACCTT TCTGAGAATT CTAGCGATCC TACCAGGGCT 
GAGGAATTAC TCACTATACA GAAGACCATT CACTCTCCTA GGTTTAAGGA TGACGGCACC 
GTCGTGTCAA CTGAATCATT CCGCTGCCAA CTAACCTCAA GGGGAGCTGA AGTATTGCCA 
CACACCAATG AAATAGGCAG TCAGGAGCAT ACGAGTTCTT TCAACACCTT CAAAGTGGTA 
TGCAAGACTT CAAAACAAGC ATTAGTTGTC GCACAATCTC AGGAAGGAAA ATCACATGGG 
CTTTCTCCAG CGGCTGCCGT AGCTATCACA TTGAATGTAA GAGATTTGCA GAAAATCGAG 
GGGAAGATCT ACTGCGGTCT ACCTTTGTCA GTTCCAAGCC TGCTTCCATT TCACATGGAT 
GGTACGTTCG CCATTAACGA AGATCGAAGA AGTCTACAAT CACCGACACC ACATGGGATG 
ACTGTTTTTG AAAGATGGAA TCATATATTG CTCTCTGACG TGATTTGCCG AGCATATGTC 
TCTCTTCTAT CAGACAAAGA GTTCATTCAA CAGATCAAGA ATACATACGG GACAGATGTG 
TCGCCAGAGT TGTTGTGGCC TGATGTTGAT AAAGTACCTG AGAATTCAGT GTGTTCAGGG 
CTGTTTAATG CTTTCTACAA AACGATAATC TGCGGATTGG ATGGCAAACA ACCAGCAGTT 
TTCTGGACAA ATGACGAACC TCGTCAGTTT CACAATGTAG TTTTTCTCGG CGAGGAACTG 
CAAACGATGA AAGGAATCAA GGACGACTGT TTACAGATTT TGACAACGTA TCTGAAGGAG 
TACTGCCACA GTTGCAGTAT GGTGGAAGTG ATAGAGCTAT CAAAGAAGAC GTGGCAAGCA 
CTCGAGAGGG CAGGAGTACG TGATATGGTA GAGACTAGAA CATACAATCT GAACAGGTTG 
TTTGATGAAG TGTTCCTGCC ATATTTAGAT AAAGTGCCAC AGGATCTGAG AGATCACCTC 
ATTCTCTTTG CGCTTGAAAT GGACGGTAAC CTGCATCAAA AACTTCGAGA TGTGAAATGC 
ATCACCGTCT TAGGAGGAGC CGAACTAAAG ACATCCTGCG CCCTCATGCA TCAAGATGAC 
CCTACACTCC GTGAATTGTT TGAGCTTCAG GGAGTATTTC CAGGTGCACC ATTTGATTCA 
GCTCATCTGA CGCCGGTATT CAACCGCCTT GGTATGCGAG GACCGAATGA AGTTACTCCA 
GTTGAGATGG TTGCATGTGT TGATCTTGTT GAGAAAACTC AGTGCCCCAA GAAGGCCAGA 
GCTTTACTCG ATCTCATCGG AAAGAACCCT GGTCATTTTG ACCGTTCACT GGCCGACGGC 
AGCACTGTAA GAGATTTCAT GATGAACAAA CGATCATTCA CACTGCTTGG AATAACATGC 
CACTCTCCAG TGATCTCGGA CGTCATAGCA CAAATAGAAC ATTGTGTGAA GTATGCCACA 
TACAGAACAG AACAAGATGT AACAGACATA ATACCGATGC TCAAGGAGAT CTATGCATTT 
CTGAGGAAAG AATGGCAACG TGATTCCAAC GTCAGAAACT ATATATTGGA TCGAGTAAAG 
TGTTGTGTGT GGAATGGCAA CGGCTTTTCG GAACCAAGCA AAATGTGTAC AGGTAATCTC 
CCGTTCGATC TAGCTCCTTA CATGGCATCT ATTACCCAGG CTACTTGTGC GTTTAAAGAT 
TTGTTTGAGG CGCTTGGGGT CAAAAGGAAC ATCGACACTA ATTCGGAAGC TCTGGTTGAA 
ATACTACATA AAATCAAGAG CAAATGTGAC GTCCTAGCAG CAAATGTAAT CTTCCAACAA 
AAACAGCGCC AACTTGTACT GCAGATCCTT CACCATCTAC ATGCCACAGG AGAGCTTTCA 
AAAGAAGCTC GGGTACGCTT ACTTGTTCCA TCGCAAAGAG GAAAATGTCA TCTCATTCCA 
GTTGATGAGA GCGCATACGT GGACAGAGAC TGGTTGAGAC AAAGCACCGA CAATGAGGAG 
ATAGATGACG ATGAAGACTT TACTTTGATT CATTCAGATA TCTCAATGGA ACTTGCAATA 
TTCCTCCACG TACGACCTGT CAGTCGACTT CTCCTAGATA CAGAAGAATT GCAGGGTTTC 
ACACCAGCTG GGCAGCATGA ACCCCTGACT ATGCGCTTGT GGAATATTCT CAAGAACAAC 
TATGTGGACA CCGCCATCGT TAGTGAAATG ATACAGAATG CGGAAGATGC AGGAGCTCAG 
GAAGTTCGCT TTCTCATTGA CATGAGAAGA AACGAGAAAG AAACCAAAAG ATTATTCGAT 
CCGGGAATGA AGTCTTGTCA GGGACCAGCC CTTTGGGTAT ACAACGATGC CGTATTCACA 
GACCAAGACT TTGAAAACAT TCTCTGTCTT GGAGGGCGTA CCAAGGAAAA GGACGCTGAA 
AAGATCGGGA AGTTTGGGAC CGGTTTCAAC TCGGTGTACC GCATAACTGA TGTTCCAAGT 
TTGGTTAGTA GGAACTACAT GCAGTGTTTC GACCCTCATA CCACACACCT TGGAAACGTT 
CTCCCGAACA AGAGTCAACC CGGTGTACGT CTCAGATTGA ATAGCTCCAA ACAACTCCGG 
AGATTCCCAG ATCAGTTCAA GCCATTTAAT GGAGTGTTCG GGCTCGATTT CAGTGGAGGA 
AATGAGCCAT TCAACTACAA CGGAACACTC TTTAGACTGC CCCTCCGTTC CAAAGTAGCT 
GCACAGAAAA GCCAAATATG CAAGGAGAGC TACGATGGGG AGAAAATGAT CAACCTGATG 
CAGAAAATGT GGGAATCATC ACAAAACCTC CTGGTTTTCA CAAAACAGGT AAAGAAGGTT 
TCCCTGTTCT ACCTTTCTGA GAATTCTAGC GATCCTACCA GTGCTGAGGA GTTACTCACC 
ATACAGAAGA CCATGCACTC TCCTAGGGTG AAGGATGACG ATACCATTGT GTCAACTGAA 
TCATTCCGCT GCACAATGAC TTCAAGGGGA AATGAAGTGA TGTCACAGAA ACCAGAAGTT 
AGTGGAGACC TGGACACTGT CAAAGTGATA TGCAAGACTT CAGAACAGGC AGCATCTCTT 
GCAAAATCTC CAGATGGCAG GGAGTGTGGA CTTTCACCAG AAGGTGCGGT TGCCTTTCCG 
TTTCATAGAC CACCAATGTC TGCATTTGAG AAGAAGATCT ACTGCTTCCT TCCCCTCTCA 
ATTAACAGTA TGCTCCCAGT GCATATCAAC GGATCTTTTG CTGTAAAAGA GGACCGGCGG 
AGTCTTCACA TGCCAGTGCC CGATGGAAGG TTGATATCTG AGAAATGGAA TCACATTATT 
CTGGCAGATG TGATATGCAG AGCGTACGTT TCACTCTTGT CTGACAGTGA GTTCATTCAA 
CAAGTGAAGG ACACATACGG CTCGGATATG TCTCCTGAGG AGTTGTGGCC AGATATTGAT 
GACGTGCCAA ATAATTCAGA ATGTTCAGCA CTGTTTGATG CTTTCTACAA GGCAATGGTA 
CATGGGTTGG ATGGCCGTGA ACCATCATTG TTCTGGAAAG ATGGCAAATC GCATCAGTTC 
ACGAATGTTG TGTTTCTTGG TGAAGAAGTT CAAAAGGAGA CATCTGTCAG TGATGTGGTA 
ACAAAGGTAC TAAGCGACCA TCTCCCCTCA ATGGAAGTGA TGTCATTGAC CGAGAAAACT 
TGGAGAGCCC TAAGAAGAGT ACAGCTGATA AATGAAGTTC TCAAGAGGAC CTATGATTTC 
AACAGGTTCT TCGATGAAGT TTTTCTACCA CAAATCAGTC AGGTGCCGGA CAACATCAGA 
AACCAACTGG TTCTGTTTGC ACTCAATAAC AGTGAAGACA CACTGCAGCA GAAACTAAAA 
GATGTGGAAT GCATACCAGT ACAGGGAGGA ACTGAACTGC GGAAACCTTG TGATCTAGTA 
CACCCGAAAG GCAAAGCTGC GCCTTTGTAC TCGGAGTCTG ACAAGGTTTT CCCTCTGGGA 
GAAAAATTCA ATGCCACCGA CACATTGGCG TCACTTAGTA AGCTAGGAAT GGTGAATGAC 
TATCTTCATG AGGATATTTT CGTCGAGCGA TGCGAGACTG TTCACATTCT GCATTCAAGC 
TGCCGGGAAG TAGCTCAAAC GAGATGCCAT GCTGTACTGA ATTATCTGTC GGAGATGTTG 
AGGAGTGATT GGAATGAGAA AAAAACCCTC AAAGAACGGC TTTCGAGTAT CCCATTCTTG 
CCTATTTTGT GTGAGAAAAA TAGAGACCAA GCTCTACCGT GGGTGGAAAC AGACTCTCTT 
GCTTATGCAT CTGCGAATGA TGTGTACTCT CATTCTAAAA CATATTTGGT AAGCAGCACG 
TCAAACGTTG TGGATGAATC AGAAGAGTGG AAGCTTCCCT TGACAGTCGA GCATTTTCTT 
GGTCTTGTAG ATAAATATCC CTCTGTCCAA GATGTACTCA GGCAGTTTTC AAGCTTTCGT 
AGTCACTACG AAACGAATAA TGAATGCATT TCTGGTCATC AGTTACAAAT GATATGCAAG 
GCGGTCTACG AGCGTGTTGA AATTGCTTGT GAAGACAATA GTCCTGACAT CTCCAACGAA 
GATCTTCAGA ATCAATCATT CTTCCTTGTA GATCGCAAGT TTGTAGTGGC CTCTATGGTT 
GTCGATAGAG GCAGAGATCT TCCACCGTAT CTATACAAAG CTCCCCAGTT CCTGAAACAA 
TATCCAATTC TTCTGGAACG TGCAGTATTG AAGAGGGAGT TCGATGCAGG AGACTATCAA 
TGTGTTCTCC AATCTATATA TGAGGAAAGC AATGGGAAAC CTTTAGACGA CGAGGTCTTG 
GAGGTTGCTA TTTTAGCTGC CGATCGACTA GCCAGTTTCC TCAAAGATGA TGATGATGGG 
AGACGGTCTC CAAATGCATT TCTCCCAGAT GAGCACAAGT GCATGAGGCC TGTATCAGAT 
CTCTGCTACA ATGACATTGA TTGGTTTGAA TACGACGAAA TCGAACTACA TAAGTGTCAC 
ATGAGAATTA GCTTGATGCT TGCAAAAAAG CTGAAAGTGA AGGATGTTCG ACAAATGATG 
CTCCGGCCAT ACGAAATGGA TTTCTCAGGG GAAGAGTTTG GCCAACATGA GAAACTTACA 
ACCCGTATCA AGCGGATTCT CGATTCGTAC CCGTCTGATG AAACAATTCT GAAAGAACTT 
CTTCAGAATG CAGACGATGC TGGAGCAACT GAAATCCACT TTGTCTACGA TCCTCGCTCA 
CATAGTGAAA ATAAACTTCT AGCAGATAGC ATGAAAGCTT TGCAGGGTCC AGCTCTTTGT 
GTCTTCAACA ACCAATCTTT TACAGATGAA GATATTCGTG GGATACAGAA TCTTGGAAAA 
GGTAGCAAAG CTGATCATAT GGAAAAGATA GGCCGTTATG GGGTCGGTTT CAATACGGTG 
TATCAACTTA CGGATTGCCC AAGTTTCATC TCCAAAGGTG AACGATTATG CATGTTTGAC 
CCTCTGCTGA AGTATATTCC TGGCGCAACA TCGACTAAGC CGGGCCGTCA GATAAGAGTT 
AATGACAGTG TACGTTCTTC GTATCCAGAT ACCTTTCAAT GTTACTTGGA AGACATTCTT 
GCAGATGGAA AGAAGGACTA TACTGTTTTT CGTTTTCCAT TGCGGGAGGA AGCTTCAACA 
CTTAGCAAAA ACGTATGGCG AGCTGAAAAG ATTCGGAAAT TACTACAAGA CTTTGAGAAT 
GCTGCATTCG AAAGTCTTTT GTTTCTGAAA AACATCACTA AAATCAGCAT CTCAGAAGTA 
ACAAGCTCAG GAGCGATTGG CTCTACTCAT GCTATCACAG CAAAGATAAC CGAAGTGGAA 
GGTGAAAAGA AAAATGATTT CCTGCGCAAA GTTCAAGATA TCAGTAGAGC AGCAAAGGAT 
GACGGTACCT GGCAAACCGA ACCAGCAGAA GTCATATACC AGATGAGATT GAAAGGGAAA 
GGAAGAGATG AGGATTGGGT AATATGTCAG CAAGTAGGAA GTACAGACAC GGCTGAAGAA 
AACATAATGC GAAAACGTAA ACTACTTCCA GTGGGGGCTG TTGCTGCCCG TCTGACAGGT 
AAAAAAGAGG AACTCAAAGG AACGGCTTTT TGCTTCTTAC CCCTTCCCAT TGCAACGGGA 
CTTCCAGTTC ACGTACACGG ATACTTCGCC TTAGACCATG AGAGTAGAAG ACACTTGTGG 
GAAGGTAGCG AAACAGATGA ACACACAAAG TGGAACTTTC ACCTCATCCA GAAAGTTATT 
GCACCGGCTT ATGTGAAGTT GCTGTTGTGG ATACAGGGAT TCTTTGTCAG GAAATATGAA 
TGGTCAGGAT CGAAGTCATG TCAACAAAAA GCTCACACTT TGAAACTCCT TCAGAAATAC 
CATACCTTCT TTCCTAACAT CGGCGAGAAA GGGTCTTACT GGAAAACACT TGAAGCCACT 
GTATACGAAA TTGTTGCCTC GGAAGCCCTG ATGCTATTTC CTTCTCTATA CAGAAAAGAT 
GATGGCAACT GGGACTTTTT TCTAACATGG AAGACCCCAG TCGTGCACAT GCAGCCTCCA 
GACGAATCAA AGGTGGGATA TTTCAACACA CTGCCAAACC AAATGAGTGA TCACTTCACA 
TTCCCTACAA GTAGCTCCTT GAAGGCTGAG ATGCAAGAAA ATGTGAAAAA AATGAAGGAG 
ACACTGATCG AAATCACATT TCCATTACTA GAGTCTCCGA CATGGTTAAT GCACAGGCTC 
CGTAATGCAT GCAAGCATCT CAATCAGGAA ACCATGAACC ATGAATCAGG AAACTATAAA 
GTTCTTGAAG TTCTACCTTC AAATGTGGTG TCTTTCCTTC GCGGAGACAA CCCTCTTCAG 
AGGCAATTAC CAATGCCACT TTCAGAATCA AGGATCCACG ACGAGGGGAG ACTGAATGGA 
CTCATAGATT ACTGCGAGAA GGGAACAAAA TCTAACTTCA AAGAAAGTCT CCACGGATTA 
CCATTGCTCT TCACTCAGGA TGGGAACTTG AGGAGGTTAT CACGTGATGA GCGAGTCTTT 
GCATCAGAAT TCTGCGATCT TTTCAAGCCT CAGAGTCATA GGTTTTTACA TATCGACTTT 
CTAGACACTC TACCACGAAA TGCCGATGTC TTTCGTGAGC TTGATGTGAG TGCAGTATCT 
GCGCTTCTTG ATGATTGCAA ATCTTTTCTA AAGTGTGCTT CCGATGAAAT AATAACGCTT 
CGGAACTACC TTCCATCTGA ATGGTTTGAA CGTTTGTGGA GTCTTCTTGA ATCCTTATGC 
ACAGAAACGA ACAAGTTGAC TGATGTTGCA CACGATATCA GAGTTGAACT TGGCCGTTGG 
GCGATATTAC CTGTCACAGA AAGAAAAACT GAAGGTCGTC CGAGAATTAG GCTAGTGGCA 
CCTATCAGTT TATCCTCTAC CATAATGGTC CAACCAACCA AGTTGGAAAA GAGTTGCTGG 
CCAATCTACT CAGCACTTGA ATCACTGAAC GTCTACAAGG TCGAAGAATC CATTCTTCAC 
GAAGACAAGA ATAGCTTAAT ACATCATCTC GTCACACACA AGGGCGATAC CACGAGGGTT 
TTAGATGCCT TGATGTACTG GGTTGATAGA GACGTCAAGA GATTCAGAAA CCTCGAAGCT 
GATCAACATG AAGAAATCCT GAGATTCTTC AGTAACTGTG AAAGCGAAGC TGATCTCAAA 
AGGTTGAAGA GCCTGCCTTG CTTCAAATCG ATTGCAGGTG ACTTTGTCAG TATCAGAGGT 
TTTGACACAG TTCTTGTCCT CCCAACAAGT ACAGAAATCA CATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC764024 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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