NHE cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	NHE
Entrez Gene ID	754777
Full Name	sperm-specific sodium proton exchanger
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001098457.1	NP_001091927.1	sperm-specific sodium proton exchanger precursor
XM_030988788.1	XP_030844648.1	sperm-specific sodium proton exchanger isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Tu Q, Cameron RA, Worley KC, Gibbs RA, Davidson EH
Genome research22(10)2079-87(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NHE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NHE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSd20357	NM_001098457.1 Latest version!	Strongylocentrotus purpuratus sperm-specific sodium proton exchanger (NHE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSd54957	XM_030988788.1 Latest version!	Strongylocentrotus purpuratus sperm-specific sodium proton exchanger (NHE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NHE ( NM_001098457.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> NP_001091927.1 Strongylocentrotus purpuratus sperm-specific sodium proton exchanger (NHE), mRNA.

Price & Availability↑

CloneID	OSd20357
Clone ID Related Accession (Same CDS sequence)	NM_001098457.1
Accession Version	NM_001098457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569600000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	sperm-specific sodium proton exchanger precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF424083.1. On or before Jun 3, 2007 this sequence version replaced XM_001201238.1, XM_001187035.1. ##Evidence-Data-START## Transcript exon combination :: EF424083.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01103178, SAMN01103179 [ECO:0000350] ##Evidence-Data-END##

ATGAAGAAGA GAGTCGTGAA ATTGCGGGAA CTTGTCCCTG CTGTGGCAGC ACTCGCTGTA 
GCGGTACTCA TCCAGTCTGC CACCGGGAGT TCTGGGGGAT CGGGACATAC ACCGACGACA 
CAAGCTACAC ATGCCGATGA TCATGATTTG ACAACCCACA ATGGAACCGA AGAGCATGAT 
GATGGACATG ATGATGGACA TGATGATTTA CATGCCCATG CCCCGAAAGT GATTGTCTTT 
ATATCTGGAA GCTGTCTCTT TGGTGCCATC TCAAGGTCCC TCTTTAAGAA GCTGCCGATC 
CCATACACAG TGGTCCTGCT CATCCTGGGG GCCATCTTGG GTGTGGTCGC ATCCAACGTC 
CCCCTGGTCG AGGAGCACAC CAGGGACGTG GCCCACATGG ACCCCCACGT CCTGCTCCAG 
ATATTCCTCC CTGTCCTCAT TTTTGAGTCT GCCTTTGCCA TGGACGTCCA CACATTCATG 
AGGTCTTTCT CACAGGTTTG TATACTTGCC CTGTTTGGTT TGGTAGTGGC GTCAGTCCTG 
ACTGCTGTTC TGGCAATGAA CCTGTTCAAC TACAACTGGA ACTTCAGTGA AGCCATGATG 
TTTGGCGCCA TCATGAGTGC GACGGATCCC GTCGCTGTGG TCGCCCTGCT CAAAGACCTT 
GGTGCATCCA AACAGCTTGG GACCATCATT GAAGGTGAAT CACTTTTGAA TGACGGCTGT 
GCCATCGTCA TCTTCAACGT TTTCATGAAG ATGGTCTTCT TCCCTCAGCT AACATCAACA 
GTTGGCCAGA ACGTCCTATA CTTCCTGCAA GTGGCCGTCG CTGGACCACT GTGGGGCTAT 
GCCGTCGCCA AAGTGACGGT CTTCTTTCTG TCACACATTT TCAACGACGC CCTGGTCGAG 
ATCACCATCA CCCTGGCCGC CACCTACCTC ACCTACTACA TCGGCGACAT CTGGCTCGAG 
GTGTCCGGCG TCCTGGCCGT GGTCGTGCTC GGTCTCATCG TCAATGCTGA GAAGACGTCA 
ATCAGCCCAG AGGTTGAGGT CTTCCTGCAC AGGTTTTGGG AGATGCTTGC TTACCTAGCC 
AACACTCTTA TCTTTATGAT GGTGGGCGTT GTCGTTACCC AGAAGGCCCT GGTCGCTGTT 
GATAAGATGG ATTGGTTCTA TCTCATCATC CTCTATCTTG CCATCACTAT CATCAGGGGC 
ATGGTGATCA GTCTATTCAG TCCTATTCTG TCGAGGATTG GTTACGGTCT CACCTGGCGC 
AATGCGGTCA TCATGACGTG GGGCGGGCTC CGAGGTGCAG TGGGTCTGGC CCTGGCCCTT 
GTGGTGGAGA ACTTGGCCGG GAACGATGTC ATCGGGAGCA AATTCCTCTT CCATACGGCG 
GGCATCGTGG TGCTGACACT TGTCATCAAT GCCACAACCA TCCAGACTCT ACTGAGAATC 
TTGGGCATGA GTGACATCTC CATCCCGAAG CGTCTGGCCA TGGCCGGTGC CGTGAGACGT 
ATCCATGAGG GTCAAAACCG GACCCTCAAC ATGCTCAAGT CTGACCGCTT CCTTGCCGAT 
GCAGACTGGG ACATCGCTAC AGCCGCTTGT GAGATCAGCG ACCCATATAG CGCCTTGAGT 
GATGACGAGA ACGCACCAGC CGATGAATTG ACCCTTGGTG AAAGGAAGAG CGTCTGTCCA 
GGCTGCAAGG CCATGGTACC CAACGAACCA TCCCCTAGAG AGTTTGCGGA CATGATGGAA 
GAAGCCAGGC TCAGGATGCT GAAAGCAGAA AAGATCAGCT ACTGGAAGCA GTTTGAGCAT 
GGTATGCTTG CCAGGGAGGC ATTGCGTCTC CTGGTTCAGC ACGCCGAGGT AGCGGCCGAT 
GAGAAGGACC AGTTCATCTT GGTCGATGAT CTCAAGAAGT CATGGCAAAT CAAAGGCATC 
TACCCCTGGC TGAAACGCAA GCTTGAGGAC CTCATCAGCG AGAAGAAGAT TGCCGCCATC 
CCCATGCCCA AGTACAAACT CGGCAAGCTT ATGTACAAGA TATGCCATCA CATGGCCTTC 
GAAGTCACCA TCAACATCGC CATTGTCCTC AACATCGTGC CCATCATTAT GGAGTTTGTC 
GTTCAGGACA AGATGGCTTC GGTATCAACC ATGGCTGCGC CCGGTTCCAC CGTCAGCTCC 
GAGCCTTCGA GCCTTCAGAA GATCGAGGAT GCCCTGAGGA TCTCCAACTA CGTCTTCTTC 
GTTATCTATG CCATAGAAGC GATAGTCAAG ATTCTTGGAT TGGGTCGTCA CTACATCGTG 
AGCCACTGGA ACAAGTTTGA TGCGTTCATC CTGGTCGTAG CTCTGGTTGA TATCATCATT 
GCAGAGACAC TTTTGAAAGG CTCAATCACC ATCAACCTGT CCTCTATCAA GGTCGTAAAG 
CTCTTCCGAC TCCTTCGAGG CCTGAGGATG CTCAGGCTCA CTAAGGCCCT GATCCCTAAG 
CTGATTCTAG TGGTCAATGG TAAAATCAAC AACCAGCTCA GCCTGGGTTA CGACGTCGGC 
AAGGGTTACA TCATCGGTGA AGAGGAGGTC GGCAAGATCA TCGATCGCAT GGTTGACAAC 
AAGAAGATCC TGAGAGAGCT CAAACATATC TCAGAGACTG GACGACTCCA GGTGGTCAAG 
GAATTAGGCC TTCTACAGCG AGAGCACCCA GGCATTGCCG TGTCAGTCAA GACTCGTCAG 
GCCATCCGCA CCATTCTCAA CCACTCCCGC GAGACTATCC ATGAACTCCA GGGGGCGGGC 
TTGCTCGACG AGATGGAAGC TCACAAACTG GAATTGACGG TTGAGATCAA GATGAAGAGA 
TTGATGAACG CTCCATCCAG TATTCCTCCT CCGCCTCCCG AGAACCTTCT CAAGAACGTT 
TCATGGCTAG CTGGAGACAT GAAACTCATC GACTTCATCA AGGCCCGTGC AAGTCTGCTT 
CACTTTGACT ATGGTGAAGT GATCGTTAGA GAGGGTGACG AGTCGGACGG CCTCTTCCTT 
ATTGTATCAG GACTCGTCAA GCTCTACGGC AAAAGTGCTT TCCTTGACCA TGACAACCCT 
CCAGTGACCG CTGGCAGCGA AGAGAATGAG GTCTTTGAGG ACTACCTGAC TGTGGGTAAC 
GTCATCGGCG AGATGGGTGT CCTCACGAAG AAACCTCGCA ATGCAACAGT CACCTGTGAA 
ACAACAGTGC AGGTATACTT CATCACTGCG GAGGACATGA ACATTGCCAT CGATACATTC 
ACCCTCTACC CATCGCTGGA GTATCGTCTG TGGCGTGTGG TCGCCATCAG AATCGCCACC 
CCCCTCATCA TGGAACAGAT GGCCTTTCAG GGCTGGACCC AGGAGAAAGT GAAGTTACAT 
CTCGAGCGTG GCTACCTTGT GGACCTAGCG GAGTCTCATT TTCAATTCAA CATCGATGCA 
ACCCTGGAAG ATGTCATTCT TATCAATGGC ACGGCTTACA ATGCTCATAC GCGTGAAGAG 
ATCCGGAGTC CCTGCCTCAT CTCGCGCACC GTTCACAAGC TCACGTTCCA GTACACTGCC 
ACAGAGGAGC CACGCCTCTT CGTGGTGCGG AACGCTGAAT ACAACGGGCC TATTCTGGAC 
GGGAGACTAG ATGTGGACAG CAAACGGTCT CTAATTTCCA TCACAGAGAT ATCTTCCAAC 
ATGTGCCTGA AGCACGCTGC CGAACTCCGT CAGAAGAACT CCAAGGTGAT GCTATCTAGG 
AAAAGCAGTG GCGCTGCTGC CAAGGAGGAA GAGGACTGCA TCCCCAACAC CTCTGATGTG 
GAGCAGGCAG CAGGGGTGTC ACCATCGGTC CCAACCAAGA CCACGCCCAA GCCTAAGAGC 
TTCCTCCCCA GCCTGGGCTT GTCCATGTCT AAGGAAAGGG TGAACGGCGA AGCGGTGGAG 
GAGTCGCCCG TGAAGACAAA ACAGGGCGAG GAAACGCCCG AAACGGAGGA AGGCGCGGCC 
CCTAGGGTCA ACGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091927.1
CDS	153..4130
Translation	MKKRVVKLRELVPAVAALAVAVLIQSATGSSGGSGHTPTTQATHADDHDLTTHNGTEEHDDGHDDGHDDLHAHAPKVIVFISGSCLFGAISRSLFKKLPIPYTVVLLILGAILGVVASNVPLVEEHTRDVAHMDPHVLLQIFLPVLIFESAFAMDVHTFMRSFSQVCILALFGLVVASVLTAVLAMNLFNYNWNFSEAMMFGAIMSATDPVAVVALLKDLGASKQLGTIIEGESLLNDGCAIVIFNVFMKMVFFPQLTSTVGQNVLYFLQVAVAGPLWGYAVAKVTVFFLSHIFNDALVEITITLAATYLTYYIGDIWLEVSGVLAVVVLGLIVNAEKTSISPEVEVFLHRFWEMLAYLANTLIFMMVGVVVTQKALVAVDKMDWFYLIILYLAITIIRGMVISLFSPILSRIGYGLTWRNAVIMTWGGLRGAVGLALALVVENLAGNDVIGSKFLFHTAGIVVLTLVINATTIQTLLRILGMSDISIPKRLAMAGAVRRIHEGQNRTLNMLKSDRFLADADWDIATAACEISDPYSALSDDENAPADELTLGERKSVCPGCKAMVPNEPSPREFADMMEEARLRMLKAEKISYWKQFEHGMLAREALRLLVQHAEVAADEKDQFILVDDLKKSWQIKGIYPWLKRKLEDLISEKKIAAIPMPKYKLGKLMYKICHHMAFEVTINIAIVLNIVPIIMEFVVQDKMASVSTMAAPGSTVSSEPSSLQKIEDALRISNYVFFVIYAIEAIVKILGLGRHYIVSHWNKFDAFILVVALVDIIIAETLLKGSITINLSSIKVVKLFRLLRGLRMLRLTKALIPKLILVVNGKINNQLSLGYDVGKGYIIGEEEVGKIIDRMVDNKKILRELKHISETGRLQVVKELGLLQREHPGIAVSVKTRQAIRTILNHSRETIHELQGAGLLDEMEAHKLELTVEIKMKRLMNAPSSIPPPPPENLLKNVSWLAGDMKLIDFIKARASLLHFDYGEVIVREGDESDGLFLIVSGLVKLYGKSAFLDHDNPPVTAGSEENEVFEDYLTVGNVIGEMGVLTKKPRNATVTCETTVQVYFITAEDMNIAIDTFTLYPSLEYRLWRVVAIRIATPLIMEQMAFQGWTQEKVKLHLERGYLVDLAESHFQFNIDATLEDVILINGTAYNAHTREEIRSPCLISRTVHKLTFQYTATEEPRLFVVRNAEYNGPILDGRLDVDSKRSLISITEISSNMCLKHAAELRQKNSKVMLSRKSSGAAAKEEEDCIPNTSDVEQAAGVSPSVPTKTTPKPKSFLPSLGLSMSKERVNGEAVEESPVKTKQGEETPETEEGAAPRVNV

Target ORF information:

RefSeq Version	NM_001098457.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus sperm-specific sodium proton exchanger (NHE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098457.1

ORF Insert Sequence:

ATGAAGAAGA GAGTCGTGAA ATTGCGGGAA CTTGTCCCTG CTGTGGCAGC ACTCGCTGTA 
GCGGTACTCA TCCAGTCTGC CACCGGGAGT TCTGGGGGAT CGGGACATAC ACCGACGACA 
CAAGCTACAC ATGCCGATGA TCATGATTTG ACAACCCACA ATGGAACCGA AGAGCATGAT 
GATGGACATG ATGATGGACA TGATGATTTA CATGCCCATG CCCCGAAAGT GATTGTCTTT 
ATATCTGGAA GCTGTCTCTT TGGTGCCATC TCAAGGTCCC TCTTTAAGAA GCTGCCGATC 
CCATACACAG TGGTCCTGCT CATCCTGGGG GCCATCTTGG GTGTGGTCGC ATCCAACGTC 
CCCCTGGTCG AGGAGCACAC CAGGGACGTG GCCCACATGG ACCCCCACGT CCTGCTCCAG 
ATATTCCTCC CTGTCCTCAT TTTTGAGTCT GCCTTTGCCA TGGACGTCCA CACATTCATG 
AGGTCTTTCT CACAGGTTTG TATACTTGCC CTGTTTGGTT TGGTAGTGGC GTCAGTCCTG 
ACTGCTGTTC TGGCAATGAA CCTGTTCAAC TACAACTGGA ACTTCAGTGA AGCCATGATG 
TTTGGCGCCA TCATGAGTGC GACGGATCCC GTCGCTGTGG TCGCCCTGCT CAAAGACCTT 
GGTGCATCCA AACAGCTTGG GACCATCATT GAAGGTGAAT CACTTTTGAA TGACGGCTGT 
GCCATCGTCA TCTTCAACGT TTTCATGAAG ATGGTCTTCT TCCCTCAGCT AACATCAACA 
GTTGGCCAGA ACGTCCTATA CTTCCTGCAA GTGGCCGTCG CTGGACCACT GTGGGGCTAT 
GCCGTCGCCA AAGTGACGGT CTTCTTTCTG TCACACATTT TCAACGACGC CCTGGTCGAG 
ATCACCATCA CCCTGGCCGC CACCTACCTC ACCTACTACA TCGGCGACAT CTGGCTCGAG 
GTGTCCGGCG TCCTGGCCGT GGTCGTGCTC GGTCTCATCG TCAATGCTGA GAAGACGTCA 
ATCAGCCCAG AGGTTGAGGT CTTCCTGCAC AGGTTTTGGG AGATGCTTGC TTACCTAGCC 
AACACTCTTA TCTTTATGAT GGTGGGCGTT GTCGTTACCC AGAAGGCCCT GGTCGCTGTT 
GATAAGATGG ATTGGTTCTA TCTCATCATC CTCTATCTTG CCATCACTAT CATCAGGGGC 
ATGGTGATCA GTCTATTCAG TCCTATTCTG TCGAGGATTG GTTACGGTCT CACCTGGCGC 
AATGCGGTCA TCATGACGTG GGGCGGGCTC CGAGGTGCAG TGGGTCTGGC CCTGGCCCTT 
GTGGTGGAGA ACTTGGCCGG GAACGATGTC ATCGGGAGCA AATTCCTCTT CCATACGGCG 
GGCATCGTGG TGCTGACACT TGTCATCAAT GCCACAACCA TCCAGACTCT ACTGAGAATC 
TTGGGCATGA GTGACATCTC CATCCCGAAG CGTCTGGCCA TGGCCGGTGC CGTGAGACGT 
ATCCATGAGG GTCAAAACCG GACCCTCAAC ATGCTCAAGT CTGACCGCTT CCTTGCCGAT 
GCAGACTGGG ACATCGCTAC AGCCGCTTGT GAGATCAGCG ACCCATATAG CGCCTTGAGT 
GATGACGAGA ACGCACCAGC CGATGAATTG ACCCTTGGTG AAAGGAAGAG CGTCTGTCCA 
GGCTGCAAGG CCATGGTACC CAACGAACCA TCCCCTAGAG AGTTTGCGGA CATGATGGAA 
GAAGCCAGGC TCAGGATGCT GAAAGCAGAA AAGATCAGCT ACTGGAAGCA GTTTGAGCAT 
GGTATGCTTG CCAGGGAGGC ATTGCGTCTC CTGGTTCAGC ACGCCGAGGT AGCGGCCGAT 
GAGAAGGACC AGTTCATCTT GGTCGATGAT CTCAAGAAGT CATGGCAAAT CAAAGGCATC 
TACCCCTGGC TGAAACGCAA GCTTGAGGAC CTCATCAGCG AGAAGAAGAT TGCCGCCATC 
CCCATGCCCA AGTACAAACT CGGCAAGCTT ATGTACAAGA TATGCCATCA CATGGCCTTC 
GAAGTCACCA TCAACATCGC CATTGTCCTC AACATCGTGC CCATCATTAT GGAGTTTGTC 
GTTCAGGACA AGATGGCTTC GGTATCAACC ATGGCTGCGC CCGGTTCCAC CGTCAGCTCC 
GAGCCTTCGA GCCTTCAGAA GATCGAGGAT GCCCTGAGGA TCTCCAACTA CGTCTTCTTC 
GTTATCTATG CCATAGAAGC GATAGTCAAG ATTCTTGGAT TGGGTCGTCA CTACATCGTG 
AGCCACTGGA ACAAGTTTGA TGCGTTCATC CTGGTCGTAG CTCTGGTTGA TATCATCATT 
GCAGAGACAC TTTTGAAAGG CTCAATCACC ATCAACCTGT CCTCTATCAA GGTCGTAAAG 
CTCTTCCGAC TCCTTCGAGG CCTGAGGATG CTCAGGCTCA CTAAGGCCCT GATCCCTAAG 
CTGATTCTAG TGGTCAATGG TAAAATCAAC AACCAGCTCA GCCTGGGTTA CGACGTCGGC 
AAGGGTTACA TCATCGGTGA AGAGGAGGTC GGCAAGATCA TCGATCGCAT GGTTGACAAC 
AAGAAGATCC TGAGAGAGCT CAAACATATC TCAGAGACTG GACGACTCCA GGTGGTCAAG 
GAATTAGGCC TTCTACAGCG AGAGCACCCA GGCATTGCCG TGTCAGTCAA GACTCGTCAG 
GCCATCCGCA CCATTCTCAA CCACTCCCGC GAGACTATCC ATGAACTCCA GGGGGCGGGC 
TTGCTCGACG AGATGGAAGC TCACAAACTG GAATTGACGG TTGAGATCAA GATGAAGAGA 
TTGATGAACG CTCCATCCAG TATTCCTCCT CCGCCTCCCG AGAACCTTCT CAAGAACGTT 
TCATGGCTAG CTGGAGACAT GAAACTCATC GACTTCATCA AGGCCCGTGC AAGTCTGCTT 
CACTTTGACT ATGGTGAAGT GATCGTTAGA GAGGGTGACG AGTCGGACGG CCTCTTCCTT 
ATTGTATCAG GACTCGTCAA GCTCTACGGC AAAAGTGCTT TCCTTGACCA TGACAACCCT 
CCAGTGACCG CTGGCAGCGA AGAGAATGAG GTCTTTGAGG ACTACCTGAC TGTGGGTAAC 
GTCATCGGCG AGATGGGTGT CCTCACGAAG AAACCTCGCA ATGCAACAGT CACCTGTGAA 
ACAACAGTGC AGGTATACTT CATCACTGCG GAGGACATGA ACATTGCCAT CGATACATTC 
ACCCTCTACC CATCGCTGGA GTATCGTCTG TGGCGTGTGG TCGCCATCAG AATCGCCACC 
CCCCTCATCA TGGAACAGAT GGCCTTTCAG GGCTGGACCC AGGAGAAAGT GAAGTTACAT 
CTCGAGCGTG GCTACCTTGT GGACCTAGCG GAGTCTCATT TTCAATTCAA CATCGATGCA 
ACCCTGGAAG ATGTCATTCT TATCAATGGC ACGGCTTACA ATGCTCATAC GCGTGAAGAG 
ATCCGGAGTC CCTGCCTCAT CTCGCGCACC GTTCACAAGC TCACGTTCCA GTACACTGCC 
ACAGAGGAGC CACGCCTCTT CGTGGTGCGG AACGCTGAAT ACAACGGGCC TATTCTGGAC 
GGGAGACTAG ATGTGGACAG CAAACGGTCT CTAATTTCCA TCACAGAGAT ATCTTCCAAC 
ATGTGCCTGA AGCACGCTGC CGAACTCCGT CAGAAGAACT CCAAGGTGAT GCTATCTAGG 
AAAAGCAGTG GCGCTGCTGC CAAGGAGGAA GAGGACTGCA TCCCCAACAC CTCTGATGTG 
GAGCAGGCAG CAGGGGTGTC ACCATCGGTC CCAACCAAGA CCACGCCCAA GCCTAAGAGC 
TTCCTCCCCA GCCTGGGCTT GTCCATGTCT AAGGAAAGGG TGAACGGCGA AGCGGTGGAG 
GAGTCGCCCG TGAAGACAAA ACAGGGCGAG GAAACGCCCG AAACGGAGGA AGGCGCGGCC 
CCTAGGGTCA ACGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHE ( XM_030988788.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030844648.1 Strongylocentrotus purpuratus sperm-specific sodium proton exchanger (NHE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd54957
Clone ID Related Accession (Same CDS sequence)	XM_030988788.1
Accession Version	XM_030988788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	sperm-specific sodium proton exchanger isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145603.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGA GAGTCGTGAA ATTGCGGGAA CTTGTCCCTG CTGTGGCAGC ACTCGCTGTA 
GCGGTACTCA TCCAGTCTGC CACCGGGAGT TCTGGGGGAT CGGGACATAC ACCGACGACA 
CAAGCTACAC ATGCCGATGA TCATGATTTG ACAACCCACA ATGGAACCGA AGAGCATGAT 
GATGGACATG ATGATGGACA TGATGATTTA CATGCCCATG CCCCGAAAGT GATTGTCTTT 
ATATCTGGAA GCTGTCTCTT TGGTGCCATC TCAAGGTCCC TCTTTAAGAA GCTGCCGATC 
CCATACACAG TGGTCCTGCT CATCCTGGGG GCCATCTTGG GTGTGGTCGC ATCCAACGTC 
CCCCTGGTCG AGGAGCACAC CAGGGACGTG GCCCACATGG ACCCCCACGT CCTGCTCCAG 
ATATTCCTCC CTGTCCTCAT TTTTGAGTCT GCCTTTGCCA TGGACGTCCA CACATTCATG 
AGGTCTTTCT CACAGGTTTG TATACTTGCC CTGTTTGGTT TGGTAGTGGC GTCAGTCCTG 
ACTGCTGTTC TGGCAATGAA CCTGTTCAAC TACAACTGGA ACTTCAGTGA AGCCATGATG 
TTTGGCGCCA TCATGAGTGC GACGGATCCC GTCGCTGTGG TCGCCCTGCT CAAAGACCTT 
GGTGCATCCA AACAGCTTGG GACCATCATT GAAGGTGAAT CACTTTTGAA TGACGGCTGT 
GCCATCGTCA TCTTCAACGT CTTCATGAAG ATGGTCTTCT TCCCTCAGCT AACATCAACA 
GTTGGCCAGA ACGTCCTGTA CTTCTTGCAA GTGGCCGTCG CTGGACCACT GTGGGGCTAC 
GCCGTCGCCA AAGTGACGGT CTTCTTTCTG TCACACATTT TCAACGACGC CCTGGTCGAG 
ATCACCATCA CCCTGGCCGC CACCTACCTC ACCTACTACA TCGGCGACAT CTGGCTCGAG 
GTGTCCGGCG TCCTGGCCGT GGTCGTGCTC GGTCTCATCG TCAACGCTGA AAAGACGTCC 
ATCAGCCCAG AGGTTGAGGT CTTCCTGCAC AGGTTTTGGG AGATGCTTGC TTACCTAGCC 
AACACTCTTA TCTTTATGAT GGTGGGCGTT GTCGTTACCC AGAAGGCCCT GGTCGCTGTT 
GATAAGATGG ATTGGTTCTA TCTCATCATC CTCTATCTTG CCATCACTAT CATCAGGGGC 
ATGGTGATCA GTCTATTCAG TCCTATCCTG TCGAGGATTG GTTACGGTCT CACCTGGCGC 
AACGCGGTCA TCATGACGTG GGGCGGTCTC CGTGGTGCCG TGGGTTTGGC CCTGGCCCTC 
GTGGTGGAGA ACCTGGCCGG GAACGATGTC ATCGGGAGCA AGTTCCTCTT CCATACGGCG 
GGCATCGTGG TGCTGACACT TGTCATCAAT GCCACAACCA TCCAGACTCT ACTGAGAATC 
TTGGGCATGA GTGACATCTC CATCCCGAAG CGTCTGGCCA TGGCCGGTGC CGTGAGACGT 
ATCCATGAGG GTCAAAACCG GACCCTCAAC ATGCTCAAGT CTGACCGCTT CCTTGCCGAT 
GCAGACTGGG ACATCGCTAC GGCCGCTTGT GAGATCAGTG ACCCGTATAG CGCCTTGAGT 
GATGACGAGA ACGCACCAGC TGATGAATTG ACCCTTGGTG AAAGGAAGAG CGTCTGTCCA 
GGCTGCAAGG CCATGGTACC CAACGAACCA TCCCCTAGAG AGTTCGCGGA CATGATGGAA 
GAAGCCAGGC TCAGGATGCT GAAAGCAGAA AAGATCAGCT ACTGGAAGCA GTTTGAGCAT 
GGTATGCTTG CCAGGGAGGC ATTGCGTCTC CTGGTTCAGC ACGCCGAGGT AGCGGCCGAT 
GAGAAGGACC AGTTCATCTT GGTCGATGAT CTCAAGAAGT CATGGCAAAT CAAAGGCATC 
TACCCCTGGC TGAAACGCAA GCTTGAGGAC CTCATCAGCG AGAAGAAGAT TGCCGCCATC 
CCCATGCCCA AGTACAAACT CGGCAAGCTT ATGTACAAGA TATGCCATCA CATGGCCTTC 
GAAGTCACCA TCAACATCGC CATCGTCCTC AACATCGTGC CCATCATTAT GGAGTTTGTC 
GTTCAGGACA AGATGGCTTC GGTATCAACC ACGGCTGCGC CCAGTTCCAC CGTCAGCTCC 
GAGCCTTCGA GCCTTCAGAA GATCGAGGAT GCCCTGAGGA TCTCCAACTA CGTCTTCTTC 
GTTATCTATG CCATAGAAGC GATAGTCAAA ATTCTTGGAT TGGGTCGTCA CTACATCGTG 
AGCCACTGGA ACAAGTTTGA TGCGTTCATC CTGGTCGTAG CTCTGGTTGA TATCATCATT 
GCAGAGACAC TTTTGAAAGG CTCAATCACC ATCAACCTGT CCTCTATCAA GGTCGTAAAG 
CTCTTCCGAC TCCTACGAGG CCTGAGGATG CTCAGGCTCA CTAAGGCCCT GATCCCTAAG 
CTGATTCTAG TGGTCAATGG TAAAATCAAC AACCAGCTCA GCCTGGGTTA CGACGTCGGC 
AAGGGCTACA TCATCGGTGA AGAGGAGGTT GGCAAGATCA TCGATCGCAT GGTTGACAAC 
AAGAAGATCC TGAGAGAGCT CAAACATATC TCAGAGACTG GACGACTCCA GGTGGTCAAG 
GAATTAGGCC TTCTACAGCG AGAGCACCCA GGCATTGCCG TGTCAGTCAA GACTCGTCAG 
GCCATCCGCA CCATTCTCAA CCACTCCCGC GAGACTATCC ATGAACTCCA GGGGGCGGGC 
TTGCTTGACG AGATGGAAGC TCACAAACTG GAATTGACGG TTGAGATCAA GATGAAGAGA 
TTGATGAACG CTCCATCCAG TATTCCTCCT CCGCCTCCCG AGAACCTTCT CAAGAACGTT 
TCGTGGCTAG CTGGAGACAT GAAACTCATC GACTTCATCA AGGCCCGTGC AAGTCTGCTT 
CACTTTGACT ATGGTGAAGT GATCGTTAGA GAGGGTGACG AGTCGGACGG CCTCTTCCTT 
ATTGTATCAG GACTCGTCAA GGTATACTTC ATCACTGTGG AGGACATGAA CATTGCCATC 
GATACATTCA CCCTCTACCC ATCGCTGGAG TATCGTCTGT GGCGTGTGGT CGCCATCAGA 
ATCGCCACCC CCCTCATCAT GGAACAGATG GCCTTTCAGG GCTGGACCCA GGAGAAAGTT 
AAGTTACATC TCGAGCGTGG CTACCTTGTG GACCTAGCGG AGTCTCATTT TCAATTCAAC 
ATCGATGCAA CCCTGGAAGA TGTCATTCTT ATCAATGGCA CGGCTTACAA TGCTCATACG 
CGTGAAGAGA TCCGGAGTCC CTGCCTCATC TCGCGCACCG TTCACAAGCT CACGTTCCAG 
TACACTGCCG CAGAAGAGCC ACGCCTCTTC GTGGTGCGGA ACGCTGAATA CAACGGGCCT 
ATTCTGGACG GGAGACTAGA TGTGGACAGC AAACGGTCTC TAATTTCCAT CACAGAGATA 
TCTTCCAACA TGTGCCTGAA GCACGCTGCC GAACTTCGTC AGAAGAACTC CAAGGTGATG 
CTATCTAGGA AAAGCAGTGG CGCTGCTGCA AAGGAGGAAG AGGACTGCAT CCCCAACACC 
TCTGATGTGG AGCAGGCAGC AGGGGTGTCA CCATCGGTCC CAACCAAGAC CACGCCCAAG 
CCTAAGAGCT TCCTCCCCAG CCTGGGCTTG TCCATGTCTA AGGAAAGGGT GAACGGCGAA 
GCGGTGGAGG AGTCGCCCGT GAAGACAAAA CAGGGCGAGG AAACGCCCGA AACGGAGGAA 
GGCGCGGCCC CTAGGGTCAA CGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030844648.1
CDS	487..4293
Translation	MKKRVVKLRELVPAVAALAVAVLIQSATGSSGGSGHTPTTQATHADDHDLTTHNGTEEHDDGHDDGHDDLHAHAPKVIVFISGSCLFGAISRSLFKKLPIPYTVVLLILGAILGVVASNVPLVEEHTRDVAHMDPHVLLQIFLPVLIFESAFAMDVHTFMRSFSQVCILALFGLVVASVLTAVLAMNLFNYNWNFSEAMMFGAIMSATDPVAVVALLKDLGASKQLGTIIEGESLLNDGCAIVIFNVFMKMVFFPQLTSTVGQNVLYFLQVAVAGPLWGYAVAKVTVFFLSHIFNDALVEITITLAATYLTYYIGDIWLEVSGVLAVVVLGLIVNAEKTSISPEVEVFLHRFWEMLAYLANTLIFMMVGVVVTQKALVAVDKMDWFYLIILYLAITIIRGMVISLFSPILSRIGYGLTWRNAVIMTWGGLRGAVGLALALVVENLAGNDVIGSKFLFHTAGIVVLTLVINATTIQTLLRILGMSDISIPKRLAMAGAVRRIHEGQNRTLNMLKSDRFLADADWDIATAACEISDPYSALSDDENAPADELTLGERKSVCPGCKAMVPNEPSPREFADMMEEARLRMLKAEKISYWKQFEHGMLAREALRLLVQHAEVAADEKDQFILVDDLKKSWQIKGIYPWLKRKLEDLISEKKIAAIPMPKYKLGKLMYKICHHMAFEVTINIAIVLNIVPIIMEFVVQDKMASVSTTAAPSSTVSSEPSSLQKIEDALRISNYVFFVIYAIEAIVKILGLGRHYIVSHWNKFDAFILVVALVDIIIAETLLKGSITINLSSIKVVKLFRLLRGLRMLRLTKALIPKLILVVNGKINNQLSLGYDVGKGYIIGEEEVGKIIDRMVDNKKILRELKHISETGRLQVVKELGLLQREHPGIAVSVKTRQAIRTILNHSRETIHELQGAGLLDEMEAHKLELTVEIKMKRLMNAPSSIPPPPPENLLKNVSWLAGDMKLIDFIKARASLLHFDYGEVIVREGDESDGLFLIVSGLVKVYFITVEDMNIAIDTFTLYPSLEYRLWRVVAIRIATPLIMEQMAFQGWTQEKVKLHLERGYLVDLAESHFQFNIDATLEDVILINGTAYNAHTREEIRSPCLISRTVHKLTFQYTAAEEPRLFVVRNAEYNGPILDGRLDVDSKRSLISITEISSNMCLKHAAELRQKNSKVMLSRKSSGAAAKEEEDCIPNTSDVEQAAGVSPSVPTKTTPKPKSFLPSLGLSMSKERVNGEAVEESPVKTKQGEETPETEEGAAPRVNV

Target ORF information:

RefSeq Version	XM_030988788.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus sperm-specific sodium proton exchanger (NHE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030988788.1

ORF Insert Sequence:

ATGAAGAAGA GAGTCGTGAA ATTGCGGGAA CTTGTCCCTG CTGTGGCAGC ACTCGCTGTA 
GCGGTACTCA TCCAGTCTGC CACCGGGAGT TCTGGGGGAT CGGGACATAC ACCGACGACA 
CAAGCTACAC ATGCCGATGA TCATGATTTG ACAACCCACA ATGGAACCGA AGAGCATGAT 
GATGGACATG ATGATGGACA TGATGATTTA CATGCCCATG CCCCGAAAGT GATTGTCTTT 
ATATCTGGAA GCTGTCTCTT TGGTGCCATC TCAAGGTCCC TCTTTAAGAA GCTGCCGATC 
CCATACACAG TGGTCCTGCT CATCCTGGGG GCCATCTTGG GTGTGGTCGC ATCCAACGTC 
CCCCTGGTCG AGGAGCACAC CAGGGACGTG GCCCACATGG ACCCCCACGT CCTGCTCCAG 
ATATTCCTCC CTGTCCTCAT TTTTGAGTCT GCCTTTGCCA TGGACGTCCA CACATTCATG 
AGGTCTTTCT CACAGGTTTG TATACTTGCC CTGTTTGGTT TGGTAGTGGC GTCAGTCCTG 
ACTGCTGTTC TGGCAATGAA CCTGTTCAAC TACAACTGGA ACTTCAGTGA AGCCATGATG 
TTTGGCGCCA TCATGAGTGC GACGGATCCC GTCGCTGTGG TCGCCCTGCT CAAAGACCTT 
GGTGCATCCA AACAGCTTGG GACCATCATT GAAGGTGAAT CACTTTTGAA TGACGGCTGT 
GCCATCGTCA TCTTCAACGT CTTCATGAAG ATGGTCTTCT TCCCTCAGCT AACATCAACA 
GTTGGCCAGA ACGTCCTGTA CTTCTTGCAA GTGGCCGTCG CTGGACCACT GTGGGGCTAC 
GCCGTCGCCA AAGTGACGGT CTTCTTTCTG TCACACATTT TCAACGACGC CCTGGTCGAG 
ATCACCATCA CCCTGGCCGC CACCTACCTC ACCTACTACA TCGGCGACAT CTGGCTCGAG 
GTGTCCGGCG TCCTGGCCGT GGTCGTGCTC GGTCTCATCG TCAACGCTGA AAAGACGTCC 
ATCAGCCCAG AGGTTGAGGT CTTCCTGCAC AGGTTTTGGG AGATGCTTGC TTACCTAGCC 
AACACTCTTA TCTTTATGAT GGTGGGCGTT GTCGTTACCC AGAAGGCCCT GGTCGCTGTT 
GATAAGATGG ATTGGTTCTA TCTCATCATC CTCTATCTTG CCATCACTAT CATCAGGGGC 
ATGGTGATCA GTCTATTCAG TCCTATCCTG TCGAGGATTG GTTACGGTCT CACCTGGCGC 
AACGCGGTCA TCATGACGTG GGGCGGTCTC CGTGGTGCCG TGGGTTTGGC CCTGGCCCTC 
GTGGTGGAGA ACCTGGCCGG GAACGATGTC ATCGGGAGCA AGTTCCTCTT CCATACGGCG 
GGCATCGTGG TGCTGACACT TGTCATCAAT GCCACAACCA TCCAGACTCT ACTGAGAATC 
TTGGGCATGA GTGACATCTC CATCCCGAAG CGTCTGGCCA TGGCCGGTGC CGTGAGACGT 
ATCCATGAGG GTCAAAACCG GACCCTCAAC ATGCTCAAGT CTGACCGCTT CCTTGCCGAT 
GCAGACTGGG ACATCGCTAC GGCCGCTTGT GAGATCAGTG ACCCGTATAG CGCCTTGAGT 
GATGACGAGA ACGCACCAGC TGATGAATTG ACCCTTGGTG AAAGGAAGAG CGTCTGTCCA 
GGCTGCAAGG CCATGGTACC CAACGAACCA TCCCCTAGAG AGTTCGCGGA CATGATGGAA 
GAAGCCAGGC TCAGGATGCT GAAAGCAGAA AAGATCAGCT ACTGGAAGCA GTTTGAGCAT 
GGTATGCTTG CCAGGGAGGC ATTGCGTCTC CTGGTTCAGC ACGCCGAGGT AGCGGCCGAT 
GAGAAGGACC AGTTCATCTT GGTCGATGAT CTCAAGAAGT CATGGCAAAT CAAAGGCATC 
TACCCCTGGC TGAAACGCAA GCTTGAGGAC CTCATCAGCG AGAAGAAGAT TGCCGCCATC 
CCCATGCCCA AGTACAAACT CGGCAAGCTT ATGTACAAGA TATGCCATCA CATGGCCTTC 
GAAGTCACCA TCAACATCGC CATCGTCCTC AACATCGTGC CCATCATTAT GGAGTTTGTC 
GTTCAGGACA AGATGGCTTC GGTATCAACC ACGGCTGCGC CCAGTTCCAC CGTCAGCTCC 
GAGCCTTCGA GCCTTCAGAA GATCGAGGAT GCCCTGAGGA TCTCCAACTA CGTCTTCTTC 
GTTATCTATG CCATAGAAGC GATAGTCAAA ATTCTTGGAT TGGGTCGTCA CTACATCGTG 
AGCCACTGGA ACAAGTTTGA TGCGTTCATC CTGGTCGTAG CTCTGGTTGA TATCATCATT 
GCAGAGACAC TTTTGAAAGG CTCAATCACC ATCAACCTGT CCTCTATCAA GGTCGTAAAG 
CTCTTCCGAC TCCTACGAGG CCTGAGGATG CTCAGGCTCA CTAAGGCCCT GATCCCTAAG 
CTGATTCTAG TGGTCAATGG TAAAATCAAC AACCAGCTCA GCCTGGGTTA CGACGTCGGC 
AAGGGCTACA TCATCGGTGA AGAGGAGGTT GGCAAGATCA TCGATCGCAT GGTTGACAAC 
AAGAAGATCC TGAGAGAGCT CAAACATATC TCAGAGACTG GACGACTCCA GGTGGTCAAG 
GAATTAGGCC TTCTACAGCG AGAGCACCCA GGCATTGCCG TGTCAGTCAA GACTCGTCAG 
GCCATCCGCA CCATTCTCAA CCACTCCCGC GAGACTATCC ATGAACTCCA GGGGGCGGGC 
TTGCTTGACG AGATGGAAGC TCACAAACTG GAATTGACGG TTGAGATCAA GATGAAGAGA 
TTGATGAACG CTCCATCCAG TATTCCTCCT CCGCCTCCCG AGAACCTTCT CAAGAACGTT 
TCGTGGCTAG CTGGAGACAT GAAACTCATC GACTTCATCA AGGCCCGTGC AAGTCTGCTT 
CACTTTGACT ATGGTGAAGT GATCGTTAGA GAGGGTGACG AGTCGGACGG CCTCTTCCTT 
ATTGTATCAG GACTCGTCAA GGTATACTTC ATCACTGTGG AGGACATGAA CATTGCCATC 
GATACATTCA CCCTCTACCC ATCGCTGGAG TATCGTCTGT GGCGTGTGGT CGCCATCAGA 
ATCGCCACCC CCCTCATCAT GGAACAGATG GCCTTTCAGG GCTGGACCCA GGAGAAAGTT 
AAGTTACATC TCGAGCGTGG CTACCTTGTG GACCTAGCGG AGTCTCATTT TCAATTCAAC 
ATCGATGCAA CCCTGGAAGA TGTCATTCTT ATCAATGGCA CGGCTTACAA TGCTCATACG 
CGTGAAGAGA TCCGGAGTCC CTGCCTCATC TCGCGCACCG TTCACAAGCT CACGTTCCAG 
TACACTGCCG CAGAAGAGCC ACGCCTCTTC GTGGTGCGGA ACGCTGAATA CAACGGGCCT 
ATTCTGGACG GGAGACTAGA TGTGGACAGC AAACGGTCTC TAATTTCCAT CACAGAGATA 
TCTTCCAACA TGTGCCTGAA GCACGCTGCC GAACTTCGTC AGAAGAACTC CAAGGTGATG 
CTATCTAGGA AAAGCAGTGG CGCTGCTGCA AAGGAGGAAG AGGACTGCAT CCCCAACACC 
TCTGATGTGG AGCAGGCAGC AGGGGTGTCA CCATCGGTCC CAACCAAGAC CACGCCCAAG 
CCTAAGAGCT TCCTCCCCAG CCTGGGCTTG TCCATGTCTA AGGAAAGGGT GAACGGCGAA 
GCGGTGGAGG AGTCGCCCGT GAAGACAAAA CAGGGCGAGG AAACGCCCGA AACGGAGGAA 
GGCGCGGCCC CTAGGGTCAA CGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Genome research22(10)2079-87(2012 Oct)
Tu Q,Cameron RA,Worley KC,Gibbs RA,Davidson EH

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NHE cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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