LOC752284 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC752284
Entrez Gene ID	752284
Full Name	pseudopodium-enriched atypical kinase 1
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011664287.1	XP_011662589.1	pseudopodium-enriched atypical kinase 1
XM_031000296.1	XP_030856156.1	inactive tyrosine-protein kinase PEAK1
XM_011664286.1	XP_011662588.1	pseudopodium-enriched atypical kinase 1

Pathways from BioSystems
Gene Ontology

The following LOC752284 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC752284 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd28151	XM_011664287.1 Latest version!	Strongylocentrotus purpuratus pseudopodium-enriched atypical kinase 1 (LOC752284), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSd28151	XM_011664286.1 Latest version!	Strongylocentrotus purpuratus pseudopodium-enriched atypical kinase 1 (LOC752284), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSd66259	XM_031000296.1 Latest version!	Strongylocentrotus purpuratus inactive tyrosine-protein kinase PEAK1 (LOC752284), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC752284 ( XM_011664287.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011662589.1 Strongylocentrotus purpuratus pseudopodium-enriched atypical kinase 1 (LOC752284), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSd28151
Clone ID Related Accession (Same CDS sequence)	XM_011664287.1 , XM_011664286.1
Accession Version	XM_011664287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011998478.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAAG ATCTGAAAGC TACCACTGCA GCCTGTTCAG CTTTTGAACA GCATCGCTTC 
AAAAGCCACC TCTGTGTTCA CTGCTTCAAG CCCAAGTTGG CTCATGTAGA GAAAGTCGAC 
AAGAAGATGG ATACCACAAA GATTCTCGCC CTTGACAACA ACATCAACAA TGTGCAAGAG 
AATGATGGGA AGGCTGCGAA ACAGCAGAAG TATGACATTC CCAAAGTCAT CATTCGCAAC 
CAGAATAACC TCCAGAAGGA TGTGAAAGGG CGGGGCGAGA GACAGGCTGA AAGGAATAAC 
AGGAAGAGGG GTAAAGAGAG AACTAAAGAA TCGTTACCCC ACAATGCAAA GCGTGATGTG 
ACGAGTAAAG CCACGAAGAA TGCCTTGACC TTTAACATCC CTTGTGAAGC AGAATGCCCG 
TCTGGTTTGG ACAACACGGA ATCATCTAGA AATGAATCGA CACAAGCTGG GATAAAGAGC 
TCCGAATCAT GTTCAGAGAA TGGAAGCATC AACATCAATG TTGGACAGGG ACAGGAGACC 
AATGGCAATA TCTACCCGGA TAACAACCCA TCAGACAGCG AGCTAGAGCT GAGTGGTAGT 
TACAGTGAGC AGTATAGGCG AGCATTGCGC CCCGTACAGA AGGCAGGAAA CCCGTCTAAG 
ACCAGAGGCA GGAGCCGCCA TGATCCAGAG CTCCCAGAGT TTGTGAAAGT CGCCAAAAAT 
CTTAGACACA TTCAGAGCAT GGGGAATACT TCTTCAGCAG AGGTTCATAG CAGCAATAAC 
AACAACCATT CTGTTATCGA GACAGAAGAG ATGCTCCCAA ATCTGCCTGA CTTGCCAAAC 
GTGGTTGGAA GTGATGCCAG TGTAGAGGAG CTACAGAAAT TAGTGGATTC CAAATCCAAT 
CTGCCTATGA ATTTTGTCAT GAACTCTCAG TCTACGCTGA CAGGTCACGA GACTCTCCCA 
AAGTCCGTAG GAAAGTCAAG GCCATCTATA AAGCCACTCA CAAAACACAT AAGACAGACT 
TCCGATCCGG AGGTTTTCAT GCCATCTACG AACAAAAAAA TTGTGGTAAA CGTCAGCACT 
TCGACAGGAG AAAACATGAA AGATGTGAAG ACTGAGAATG TAGTGGTGAG AAACAGCCAG 
CTCAGTTCAG ATGTTTATTT TGGCAAGCCT CCGGTCACGT CTCCCCAGAA ACATGCAGGC 
GACGATGAAC AACAAGATTT GACATCCCGA TATGGACATA TTGCAGATTG TGTCACAATT 
ATTTCACAGA GTGGGGAGGT ATCTCATCGC TATGAGATAG ACAAAGGAGA CAGTGGTAGT 
GCTGAGATGG ATAATGGTTC AGGTTCCCAG TCTCCTACAG TGAGAACAAA ACCCTACAGG 
GTTGTAGATT TGAATGATAA TCAGGCGAAA AAGTCAAGCA GTAATGGTAC TTCATCAAAT 
AGTGGTAGGA TGTCTCATGA ACTAGATGAA GGTCTTGGAT TGATCCAGCA GGAGGATGGT 
GGAGCTAGCA CCTCAATGGA TCCATCAAGT CGCTACTCAA GGAGAAATGT TGCTCTTGTT 
GATGGCATCT ATACTGCATC TCAGTTGAAC ACAGACTCTG GTATCAACGA TTCCATGGAA 
TCCAGTGATT CTAGTGATGC ACCAAAGCAC GAAACACCGC CTGCACAGAG CAGTAAAAGA 
AATACGCCAC CATACCCTAT CATTCTTCAT GATGAACAGC CTCCAGATAA TAGAAAAAAT 
AAACGAAATT CAGGACAGTA TAAGGTTCCA ATATTCATTA TGCCAAAGGC GTTCAACAGC 
TCAGAGTGCG ATGGTACCAA AGATAGTAAT GGTGGTGAGG TAGATGCTCA AGTACTGCAG 
GGTCTTAATC AAAGCGTTGA TTATCTCCAG CCTAAAGAGA AATCCAGTGA AAAGCAAGTT 
ACCGCTCCAA GCAGAGAGGA AACTGCTAAG AATGAAAGTT CACAGAATCT ACTGGAGTCA 
AAGAGAGTGT CAATGAGTGA TGAGACATTA AGAGAAACCA GGAGACCATC TCGATCAAGG 
TCAAAAACTC ATCCAGTCTT GGAGGAGGAA TTGTCAAATG GTTCGAGTGG GAAAGACCCA 
GATATACCAG ATTTTGGTCA GACTAGGCTT AAGAGGGTGG CTCCAAGGCC CCCATCGATG 
GAACTCACTG AGCAGAACTT TGGTAGGGAG AGGGATGACA GGCTGGATAG CTTGCCCCAT 
CGTTCCCCGC CCCCTCCCCC TCAAAACAAA AAAGAAAAGA AAAAATCAAT AGACAGAAGT 
TCCTCAGACC TATCGGATGA CAATGACAGA TCATCATATC CGCAGGCATC CAATACTGAC 
AAACTTAAGG TAGAAACTCG CATCAGTGGG AGCTCGGGAA GGGGTGATGA AGTAAGAAGA 
GAGATGTCCC CCACGAGACA CAGAGACATG TCTCCTCTTA GAAATCAACA TGAATCTAGA 
AAGGCGACTC CAGTCAAGAA AGAGAGTAAT TTTCGAGCGA GGCTCCGGGA GTTTTCTCCT 
ACCGGACGAA GGGAGCTGTC ACCAGTGAGC AGAGGGAAAC AGAAGGAGAA TCAACCCAGT 
GCCCCACCTA TGAGACGAGT GGCCTCTCCT CCACCTCCTC TTAACAGGCC CCAGCCACTT 
AACCTCCCTA ACACATCGGC CACACAGGAT GCACCTGCTG TTACCACAAA GTCTAAACGA 
TCCAAGTTGC CATGGAAGAG GAAGAAGAAG CGAAATGATT CTAGCAGCCC GGAGAGGGAA 
TTCAACTCGG AGGAGGTGGA GAAATGGCTT ACGAGTTCAT TCTGTGCTCC TGCTGCTCCG 
CCAGCAGTCA ATGACTCCCA TCCAAGGAAC TCGCTGGAGC GCCTGGAATT CATCAACGCA 
TACAGGGGGC AACCAACGGT GGCAATCAAA CAGGCATCCC AAGCTACTAC CAAAGCTGCA 
TCTCCTGCCA CACCCGGTAC CAATGGGACC ACACCCACAG CCACCACACC CACCACCGCA 
ACCAATCACA ACAGGAATAG CCTCGTGGAG AGCGAAGACG ATGATGTAAC CAGAAGTGGA 
GGGAAGCGAA GGGCACCCAA ACCACCTACA CCCCTACCAG ACTTCCAGGA TGAAGACAAT 
AGTGAAGATC CAGCCCCACT CCAGAAATCC TACTCTCTGT CTCCTACCAG TAGAAGGAAG 
AGCATACCAG AGGAGGAAAA TCACCTCTAT GAAAATTTGG GTGCCAATCG AGCCAACATA 
GCCCCTCAGA AGCCAGCTAG GAGGAGACAA CGGAACAGGA CCACAGGTTT TGAGGACTTG 
CCGTCTCGCG TCAAGCGTAT GGCCCCAAAG ACTCCAGAGA AACCAACCCC TGGCGACACA 
GGAGACGATG TCATCGACGG AAACGGTGCT AAGCAACTGG TGTTTGATGA CATCCATCTC 
AGTTCCTCCC CTCCTCTGAA GTCTGCTCTC AGAAGCACAC AATCAGAACC ACTCAGGACT 
GATGCTGAAG GGAAAGTGGT TCCAAAGCGT CCAGTCAGAA TCATCGCTCC GGAAGATGCA 
GCTATTGATA CCCAATCGCA TTCCACCGTC CTCCGAAGTG ACTATACAAA CCTGGCTCTG 
GTTCCACAAG TGCATCTGGA TGATACAATC CACAAGGACG CAGAAACCCA AGTCGACGAC 
AGTCCTCAAA GGGTAGAGCC ATCTCTCGCA GATGCTCGAT GCAGTTTGAA CCCGCTGAAC 
AGTTCATCCC TTGATCAGTC TAGCCAGCGC AATCTTCTCA ATGATGTCAG TGCTGTCCAT 
CGTCAAGCCT TGACTCAACT CTCAAGGACT ACACCAAGAA CACCACTAGA CTGGACAGAG 
TGTGAGGACT CGGTCTGGAA GGAGCAGGCC ACATCAGAGC CACAGTGTCG TTCAGCTGGG 
GCAGCATTCT TTGGTGTAAG ACATCCAACC AACCCTTCCT CTCCTGCAGG CCTACTGGTG 
TGTTGTGATC CTAACAAGGC AACTCAGGTG CTGCAAATGG CTGAAATGAC CAAATCAATG 
GCAACCCATC CCAACCTCAT CTCAGTGTCC CATGCTACCA AAGACACTAT CACCATCGAT 
CCTTCACTCT TTGAGCAAAG AAAAACCAGG AGCTCTCCTC TTACTGGTGG ATCCATTGAG 
ACAGCCTTGG TCCTCACGGA TGATTTACCG ACTGGGAATG TTGCAGAGTA TGTCTCTCAA 
AATACCGAGA AGCATGAGCA CTTACCAGAG GTCTATGAGC GAGATGTTGC TTTGATGCTG 
CTGCAATTGT GCCAGGGACT GTGTGAGCTT CAGTCACGCA GCATGTGCCT TGAGAGCCTA 
AAGCTTGACC ACCTCTTACT GACTGAGAGT GGCCTTCCAG GTGGAGGACA TCGTCTAGTC 
ATCAACCACT TGATGGGTGC GGAGGTGGTT TCTCCAGCGA GGACAGACGA GAAGGAGGTT 
GCATCCAAGC TGGAGCAGAA ATGCCATGAG TTTGATGTTG GAATCCTGAT CTTTGAGTTC 
CTTCATCAGC CAAACCCTTT CTCCACCAGG ACAAGTTTGA TCATCTCGGA TTACACTGCG 
GATGATCTTC CTGGTATCCC TGTCAAGTCT TCTCTCTCCG CTGATCTACG CACAATCGCT 
GCAAAACTCC TAGCCAAAAA TCCTCAAGAT AGACTCACCT GCAATCAGGC TGTATCCCTT 
CTCCAGTGCT TGCTCTGGGG CCCACCATCT ACGGTATACA CTGATAATGC AAACACAGCT 
GAGGCCCTTA ACCAAGAATT GGAAAGGTGG CTGGTGGCTG AGCAAGCAAA GATGGTAACT 
AAAGTTGCCC TGTATTCCTT GTTGGGGCGA GCATCTTCTG AAAACAAGTC AGGACTTACT 
CTCTCAGACC AACTGCAGTG TCAGTATCTA TCTGATTCGA CAGTGGATAC TTGCAAGGAG 
GCAATGAGGA TGTTGCATGG AAAAAATGAG AGTGAAACAG TAAATGTCAT TGATTCTGAC 
CTAGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011662589.1
CDS	178..5169
Translation	MLEDLKATTAACSAFEQHRFKSHLCVHCFKPKLAHVEKVDKKMDTTKILALDNNINNVQENDGKAAKQQKYDIPKVIIRNQNNLQKDVKGRGERQAERNNRKRGKERTKESLPHNAKRDVTSKATKNALTFNIPCEAECPSGLDNTESSRNESTQAGIKSSESCSENGSININVGQGQETNGNIYPDNNPSDSELELSGSYSEQYRRALRPVQKAGNPSKTRGRSRHDPELPEFVKVAKNLRHIQSMGNTSSAEVHSSNNNNHSVIETEEMLPNLPDLPNVVGSDASVEELQKLVDSKSNLPMNFVMNSQSTLTGHETLPKSVGKSRPSIKPLTKHIRQTSDPEVFMPSTNKKIVVNVSTSTGENMKDVKTENVVVRNSQLSSDVYFGKPPVTSPQKHAGDDEQQDLTSRYGHIADCVTIISQSGEVSHRYEIDKGDSGSAEMDNGSGSQSPTVRTKPYRVVDLNDNQAKKSSSNGTSSNSGRMSHELDEGLGLIQQEDGGASTSMDPSSRYSRRNVALVDGIYTASQLNTDSGINDSMESSDSSDAPKHETPPAQSSKRNTPPYPIILHDEQPPDNRKNKRNSGQYKVPIFIMPKAFNSSECDGTKDSNGGEVDAQVLQGLNQSVDYLQPKEKSSEKQVTAPSREETAKNESSQNLLESKRVSMSDETLRETRRPSRSRSKTHPVLEEELSNGSSGKDPDIPDFGQTRLKRVAPRPPSMELTEQNFGRERDDRLDSLPHRSPPPPPQNKKEKKKSIDRSSSDLSDDNDRSSYPQASNTDKLKVETRISGSSGRGDEVRREMSPTRHRDMSPLRNQHESRKATPVKKESNFRARLREFSPTGRRELSPVSRGKQKENQPSAPPMRRVASPPPPLNRPQPLNLPNTSATQDAPAVTTKSKRSKLPWKRKKKRNDSSSPEREFNSEEVEKWLTSSFCAPAAPPAVNDSHPRNSLERLEFINAYRGQPTVAIKQASQATTKAASPATPGTNGTTPTATTPTTATNHNRNSLVESEDDDVTRSGGKRRAPKPPTPLPDFQDEDNSEDPAPLQKSYSLSPTSRRKSIPEEENHLYENLGANRANIAPQKPARRRQRNRTTGFEDLPSRVKRMAPKTPEKPTPGDTGDDVIDGNGAKQLVFDDIHLSSSPPLKSALRSTQSEPLRTDAEGKVVPKRPVRIIAPEDAAIDTQSHSTVLRSDYTNLALVPQVHLDDTIHKDAETQVDDSPQRVEPSLADARCSLNPLNSSSLDQSSQRNLLNDVSAVHRQALTQLSRTTPRTPLDWTECEDSVWKEQATSEPQCRSAGAAFFGVRHPTNPSSPAGLLVCCDPNKATQVLQMAEMTKSMATHPNLISVSHATKDTITIDPSLFEQRKTRSSPLTGGSIETALVLTDDLPTGNVAEYVSQNTEKHEHLPEVYERDVALMLLQLCQGLCELQSRSMCLESLKLDHLLLTESGLPGGGHRLVINHLMGAEVVSPARTDEKEVASKLEQKCHEFDVGILIFEFLHQPNPFSTRTSLIISDYTADDLPGIPVKSSLSADLRTIAAKLLAKNPQDRLTCNQAVSLLQCLLWGPPSTVYTDNANTAEALNQELERWLVAEQAKMVTKVALYSLLGRASSENKSGLTLSDQLQCQYLSDSTVDTCKEAMRMLHGKNESETVNVIDSDLDL

Target ORF information:

RefSeq Version	XM_011664287.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus pseudopodium-enriched atypical kinase 1 (LOC752284), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011664287.1

ORF Insert Sequence:

ATGTTGGAAG ATCTGAAAGC TACCACTGCA GCCTGTTCAG CTTTTGAACA GCATCGCTTC 
AAAAGCCACC TCTGTGTTCA CTGCTTCAAG CCCAAGTTGG CTCATGTAGA GAAAGTCGAC 
AAGAAGATGG ATACCACAAA GATTCTCGCC CTTGACAACA ACATCAACAA TGTGCAAGAG 
AATGATGGGA AGGCTGCGAA ACAGCAGAAG TATGACATTC CCAAAGTCAT CATTCGCAAC 
CAGAATAACC TCCAGAAGGA TGTGAAAGGG CGGGGCGAGA GACAGGCTGA AAGGAATAAC 
AGGAAGAGGG GTAAAGAGAG AACTAAAGAA TCGTTACCCC ACAATGCAAA GCGTGATGTG 
ACGAGTAAAG CCACGAAGAA TGCCTTGACC TTTAACATCC CTTGTGAAGC AGAATGCCCG 
TCTGGTTTGG ACAACACGGA ATCATCTAGA AATGAATCGA CACAAGCTGG GATAAAGAGC 
TCCGAATCAT GTTCAGAGAA TGGAAGCATC AACATCAATG TTGGACAGGG ACAGGAGACC 
AATGGCAATA TCTACCCGGA TAACAACCCA TCAGACAGCG AGCTAGAGCT GAGTGGTAGT 
TACAGTGAGC AGTATAGGCG AGCATTGCGC CCCGTACAGA AGGCAGGAAA CCCGTCTAAG 
ACCAGAGGCA GGAGCCGCCA TGATCCAGAG CTCCCAGAGT TTGTGAAAGT CGCCAAAAAT 
CTTAGACACA TTCAGAGCAT GGGGAATACT TCTTCAGCAG AGGTTCATAG CAGCAATAAC 
AACAACCATT CTGTTATCGA GACAGAAGAG ATGCTCCCAA ATCTGCCTGA CTTGCCAAAC 
GTGGTTGGAA GTGATGCCAG TGTAGAGGAG CTACAGAAAT TAGTGGATTC CAAATCCAAT 
CTGCCTATGA ATTTTGTCAT GAACTCTCAG TCTACGCTGA CAGGTCACGA GACTCTCCCA 
AAGTCCGTAG GAAAGTCAAG GCCATCTATA AAGCCACTCA CAAAACACAT AAGACAGACT 
TCCGATCCGG AGGTTTTCAT GCCATCTACG AACAAAAAAA TTGTGGTAAA CGTCAGCACT 
TCGACAGGAG AAAACATGAA AGATGTGAAG ACTGAGAATG TAGTGGTGAG AAACAGCCAG 
CTCAGTTCAG ATGTTTATTT TGGCAAGCCT CCGGTCACGT CTCCCCAGAA ACATGCAGGC 
GACGATGAAC AACAAGATTT GACATCCCGA TATGGACATA TTGCAGATTG TGTCACAATT 
ATTTCACAGA GTGGGGAGGT ATCTCATCGC TATGAGATAG ACAAAGGAGA CAGTGGTAGT 
GCTGAGATGG ATAATGGTTC AGGTTCCCAG TCTCCTACAG TGAGAACAAA ACCCTACAGG 
GTTGTAGATT TGAATGATAA TCAGGCGAAA AAGTCAAGCA GTAATGGTAC TTCATCAAAT 
AGTGGTAGGA TGTCTCATGA ACTAGATGAA GGTCTTGGAT TGATCCAGCA GGAGGATGGT 
GGAGCTAGCA CCTCAATGGA TCCATCAAGT CGCTACTCAA GGAGAAATGT TGCTCTTGTT 
GATGGCATCT ATACTGCATC TCAGTTGAAC ACAGACTCTG GTATCAACGA TTCCATGGAA 
TCCAGTGATT CTAGTGATGC ACCAAAGCAC GAAACACCGC CTGCACAGAG CAGTAAAAGA 
AATACGCCAC CATACCCTAT CATTCTTCAT GATGAACAGC CTCCAGATAA TAGAAAAAAT 
AAACGAAATT CAGGACAGTA TAAGGTTCCA ATATTCATTA TGCCAAAGGC GTTCAACAGC 
TCAGAGTGCG ATGGTACCAA AGATAGTAAT GGTGGTGAGG TAGATGCTCA AGTACTGCAG 
GGTCTTAATC AAAGCGTTGA TTATCTCCAG CCTAAAGAGA AATCCAGTGA AAAGCAAGTT 
ACCGCTCCAA GCAGAGAGGA AACTGCTAAG AATGAAAGTT CACAGAATCT ACTGGAGTCA 
AAGAGAGTGT CAATGAGTGA TGAGACATTA AGAGAAACCA GGAGACCATC TCGATCAAGG 
TCAAAAACTC ATCCAGTCTT GGAGGAGGAA TTGTCAAATG GTTCGAGTGG GAAAGACCCA 
GATATACCAG ATTTTGGTCA GACTAGGCTT AAGAGGGTGG CTCCAAGGCC CCCATCGATG 
GAACTCACTG AGCAGAACTT TGGTAGGGAG AGGGATGACA GGCTGGATAG CTTGCCCCAT 
CGTTCCCCGC CCCCTCCCCC TCAAAACAAA AAAGAAAAGA AAAAATCAAT AGACAGAAGT 
TCCTCAGACC TATCGGATGA CAATGACAGA TCATCATATC CGCAGGCATC CAATACTGAC 
AAACTTAAGG TAGAAACTCG CATCAGTGGG AGCTCGGGAA GGGGTGATGA AGTAAGAAGA 
GAGATGTCCC CCACGAGACA CAGAGACATG TCTCCTCTTA GAAATCAACA TGAATCTAGA 
AAGGCGACTC CAGTCAAGAA AGAGAGTAAT TTTCGAGCGA GGCTCCGGGA GTTTTCTCCT 
ACCGGACGAA GGGAGCTGTC ACCAGTGAGC AGAGGGAAAC AGAAGGAGAA TCAACCCAGT 
GCCCCACCTA TGAGACGAGT GGCCTCTCCT CCACCTCCTC TTAACAGGCC CCAGCCACTT 
AACCTCCCTA ACACATCGGC CACACAGGAT GCACCTGCTG TTACCACAAA GTCTAAACGA 
TCCAAGTTGC CATGGAAGAG GAAGAAGAAG CGAAATGATT CTAGCAGCCC GGAGAGGGAA 
TTCAACTCGG AGGAGGTGGA GAAATGGCTT ACGAGTTCAT TCTGTGCTCC TGCTGCTCCG 
CCAGCAGTCA ATGACTCCCA TCCAAGGAAC TCGCTGGAGC GCCTGGAATT CATCAACGCA 
TACAGGGGGC AACCAACGGT GGCAATCAAA CAGGCATCCC AAGCTACTAC CAAAGCTGCA 
TCTCCTGCCA CACCCGGTAC CAATGGGACC ACACCCACAG CCACCACACC CACCACCGCA 
ACCAATCACA ACAGGAATAG CCTCGTGGAG AGCGAAGACG ATGATGTAAC CAGAAGTGGA 
GGGAAGCGAA GGGCACCCAA ACCACCTACA CCCCTACCAG ACTTCCAGGA TGAAGACAAT 
AGTGAAGATC CAGCCCCACT CCAGAAATCC TACTCTCTGT CTCCTACCAG TAGAAGGAAG 
AGCATACCAG AGGAGGAAAA TCACCTCTAT GAAAATTTGG GTGCCAATCG AGCCAACATA 
GCCCCTCAGA AGCCAGCTAG GAGGAGACAA CGGAACAGGA CCACAGGTTT TGAGGACTTG 
CCGTCTCGCG TCAAGCGTAT GGCCCCAAAG ACTCCAGAGA AACCAACCCC TGGCGACACA 
GGAGACGATG TCATCGACGG AAACGGTGCT AAGCAACTGG TGTTTGATGA CATCCATCTC 
AGTTCCTCCC CTCCTCTGAA GTCTGCTCTC AGAAGCACAC AATCAGAACC ACTCAGGACT 
GATGCTGAAG GGAAAGTGGT TCCAAAGCGT CCAGTCAGAA TCATCGCTCC GGAAGATGCA 
GCTATTGATA CCCAATCGCA TTCCACCGTC CTCCGAAGTG ACTATACAAA CCTGGCTCTG 
GTTCCACAAG TGCATCTGGA TGATACAATC CACAAGGACG CAGAAACCCA AGTCGACGAC 
AGTCCTCAAA GGGTAGAGCC ATCTCTCGCA GATGCTCGAT GCAGTTTGAA CCCGCTGAAC 
AGTTCATCCC TTGATCAGTC TAGCCAGCGC AATCTTCTCA ATGATGTCAG TGCTGTCCAT 
CGTCAAGCCT TGACTCAACT CTCAAGGACT ACACCAAGAA CACCACTAGA CTGGACAGAG 
TGTGAGGACT CGGTCTGGAA GGAGCAGGCC ACATCAGAGC CACAGTGTCG TTCAGCTGGG 
GCAGCATTCT TTGGTGTAAG ACATCCAACC AACCCTTCCT CTCCTGCAGG CCTACTGGTG 
TGTTGTGATC CTAACAAGGC AACTCAGGTG CTGCAAATGG CTGAAATGAC CAAATCAATG 
GCAACCCATC CCAACCTCAT CTCAGTGTCC CATGCTACCA AAGACACTAT CACCATCGAT 
CCTTCACTCT TTGAGCAAAG AAAAACCAGG AGCTCTCCTC TTACTGGTGG ATCCATTGAG 
ACAGCCTTGG TCCTCACGGA TGATTTACCG ACTGGGAATG TTGCAGAGTA TGTCTCTCAA 
AATACCGAGA AGCATGAGCA CTTACCAGAG GTCTATGAGC GAGATGTTGC TTTGATGCTG 
CTGCAATTGT GCCAGGGACT GTGTGAGCTT CAGTCACGCA GCATGTGCCT TGAGAGCCTA 
AAGCTTGACC ACCTCTTACT GACTGAGAGT GGCCTTCCAG GTGGAGGACA TCGTCTAGTC 
ATCAACCACT TGATGGGTGC GGAGGTGGTT TCTCCAGCGA GGACAGACGA GAAGGAGGTT 
GCATCCAAGC TGGAGCAGAA ATGCCATGAG TTTGATGTTG GAATCCTGAT CTTTGAGTTC 
CTTCATCAGC CAAACCCTTT CTCCACCAGG ACAAGTTTGA TCATCTCGGA TTACACTGCG 
GATGATCTTC CTGGTATCCC TGTCAAGTCT TCTCTCTCCG CTGATCTACG CACAATCGCT 
GCAAAACTCC TAGCCAAAAA TCCTCAAGAT AGACTCACCT GCAATCAGGC TGTATCCCTT 
CTCCAGTGCT TGCTCTGGGG CCCACCATCT ACGGTATACA CTGATAATGC AAACACAGCT 
GAGGCCCTTA ACCAAGAATT GGAAAGGTGG CTGGTGGCTG AGCAAGCAAA GATGGTAACT 
AAAGTTGCCC TGTATTCCTT GTTGGGGCGA GCATCTTCTG AAAACAAGTC AGGACTTACT 
CTCTCAGACC AACTGCAGTG TCAGTATCTA TCTGATTCGA CAGTGGATAC TTGCAAGGAG 
GCAATGAGGA TGTTGCATGG AAAAAATGAG AGTGAAACAG TAAATGTCAT TGATTCTGAC 
CTAGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC752284 ( XM_011664286.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011662588.1 Strongylocentrotus purpuratus pseudopodium-enriched atypical kinase 1 (LOC752284), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd28151
Clone ID Related Accession (Same CDS sequence)	XM_011664287.1 , XM_011664286.1
Accession Version	XM_011664286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011998478.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAAG ATCTGAAAGC TACCACTGCA GCCTGTTCAG CTTTTGAACA GCATCGCTTC 
AAAAGCCACC TCTGTGTTCA CTGCTTCAAG CCCAAGTTGG CTCATGTAGA GAAAGTCGAC 
AAGAAGATGG ATACCACAAA GATTCTCGCC CTTGACAACA ACATCAACAA TGTGCAAGAG 
AATGATGGGA AGGCTGCGAA ACAGCAGAAG TATGACATTC CCAAAGTCAT CATTCGCAAC 
CAGAATAACC TCCAGAAGGA TGTGAAAGGG CGGGGCGAGA GACAGGCTGA AAGGAATAAC 
AGGAAGAGGG GTAAAGAGAG AACTAAAGAA TCGTTACCCC ACAATGCAAA GCGTGATGTG 
ACGAGTAAAG CCACGAAGAA TGCCTTGACC TTTAACATCC CTTGTGAAGC AGAATGCCCG 
TCTGGTTTGG ACAACACGGA ATCATCTAGA AATGAATCGA CACAAGCTGG GATAAAGAGC 
TCCGAATCAT GTTCAGAGAA TGGAAGCATC AACATCAATG TTGGACAGGG ACAGGAGACC 
AATGGCAATA TCTACCCGGA TAACAACCCA TCAGACAGCG AGCTAGAGCT GAGTGGTAGT 
TACAGTGAGC AGTATAGGCG AGCATTGCGC CCCGTACAGA AGGCAGGAAA CCCGTCTAAG 
ACCAGAGGCA GGAGCCGCCA TGATCCAGAG CTCCCAGAGT TTGTGAAAGT CGCCAAAAAT 
CTTAGACACA TTCAGAGCAT GGGGAATACT TCTTCAGCAG AGGTTCATAG CAGCAATAAC 
AACAACCATT CTGTTATCGA GACAGAAGAG ATGCTCCCAA ATCTGCCTGA CTTGCCAAAC 
GTGGTTGGAA GTGATGCCAG TGTAGAGGAG CTACAGAAAT TAGTGGATTC CAAATCCAAT 
CTGCCTATGA ATTTTGTCAT GAACTCTCAG TCTACGCTGA CAGGTCACGA GACTCTCCCA 
AAGTCCGTAG GAAAGTCAAG GCCATCTATA AAGCCACTCA CAAAACACAT AAGACAGACT 
TCCGATCCGG AGGTTTTCAT GCCATCTACG AACAAAAAAA TTGTGGTAAA CGTCAGCACT 
TCGACAGGAG AAAACATGAA AGATGTGAAG ACTGAGAATG TAGTGGTGAG AAACAGCCAG 
CTCAGTTCAG ATGTTTATTT TGGCAAGCCT CCGGTCACGT CTCCCCAGAA ACATGCAGGC 
GACGATGAAC AACAAGATTT GACATCCCGA TATGGACATA TTGCAGATTG TGTCACAATT 
ATTTCACAGA GTGGGGAGGT ATCTCATCGC TATGAGATAG ACAAAGGAGA CAGTGGTAGT 
GCTGAGATGG ATAATGGTTC AGGTTCCCAG TCTCCTACAG TGAGAACAAA ACCCTACAGG 
GTTGTAGATT TGAATGATAA TCAGGCGAAA AAGTCAAGCA GTAATGGTAC TTCATCAAAT 
AGTGGTAGGA TGTCTCATGA ACTAGATGAA GGTCTTGGAT TGATCCAGCA GGAGGATGGT 
GGAGCTAGCA CCTCAATGGA TCCATCAAGT CGCTACTCAA GGAGAAATGT TGCTCTTGTT 
GATGGCATCT ATACTGCATC TCAGTTGAAC ACAGACTCTG GTATCAACGA TTCCATGGAA 
TCCAGTGATT CTAGTGATGC ACCAAAGCAC GAAACACCGC CTGCACAGAG CAGTAAAAGA 
AATACGCCAC CATACCCTAT CATTCTTCAT GATGAACAGC CTCCAGATAA TAGAAAAAAT 
AAACGAAATT CAGGACAGTA TAAGGTTCCA ATATTCATTA TGCCAAAGGC GTTCAACAGC 
TCAGAGTGCG ATGGTACCAA AGATAGTAAT GGTGGTGAGG TAGATGCTCA AGTACTGCAG 
GGTCTTAATC AAAGCGTTGA TTATCTCCAG CCTAAAGAGA AATCCAGTGA AAAGCAAGTT 
ACCGCTCCAA GCAGAGAGGA AACTGCTAAG AATGAAAGTT CACAGAATCT ACTGGAGTCA 
AAGAGAGTGT CAATGAGTGA TGAGACATTA AGAGAAACCA GGAGACCATC TCGATCAAGG 
TCAAAAACTC ATCCAGTCTT GGAGGAGGAA TTGTCAAATG GTTCGAGTGG GAAAGACCCA 
GATATACCAG ATTTTGGTCA GACTAGGCTT AAGAGGGTGG CTCCAAGGCC CCCATCGATG 
GAACTCACTG AGCAGAACTT TGGTAGGGAG AGGGATGACA GGCTGGATAG CTTGCCCCAT 
CGTTCCCCGC CCCCTCCCCC TCAAAACAAA AAAGAAAAGA AAAAATCAAT AGACAGAAGT 
TCCTCAGACC TATCGGATGA CAATGACAGA TCATCATATC CGCAGGCATC CAATACTGAC 
AAACTTAAGG TAGAAACTCG CATCAGTGGG AGCTCGGGAA GGGGTGATGA AGTAAGAAGA 
GAGATGTCCC CCACGAGACA CAGAGACATG TCTCCTCTTA GAAATCAACA TGAATCTAGA 
AAGGCGACTC CAGTCAAGAA AGAGAGTAAT TTTCGAGCGA GGCTCCGGGA GTTTTCTCCT 
ACCGGACGAA GGGAGCTGTC ACCAGTGAGC AGAGGGAAAC AGAAGGAGAA TCAACCCAGT 
GCCCCACCTA TGAGACGAGT GGCCTCTCCT CCACCTCCTC TTAACAGGCC CCAGCCACTT 
AACCTCCCTA ACACATCGGC CACACAGGAT GCACCTGCTG TTACCACAAA GTCTAAACGA 
TCCAAGTTGC CATGGAAGAG GAAGAAGAAG CGAAATGATT CTAGCAGCCC GGAGAGGGAA 
TTCAACTCGG AGGAGGTGGA GAAATGGCTT ACGAGTTCAT TCTGTGCTCC TGCTGCTCCG 
CCAGCAGTCA ATGACTCCCA TCCAAGGAAC TCGCTGGAGC GCCTGGAATT CATCAACGCA 
TACAGGGGGC AACCAACGGT GGCAATCAAA CAGGCATCCC AAGCTACTAC CAAAGCTGCA 
TCTCCTGCCA CACCCGGTAC CAATGGGACC ACACCCACAG CCACCACACC CACCACCGCA 
ACCAATCACA ACAGGAATAG CCTCGTGGAG AGCGAAGACG ATGATGTAAC CAGAAGTGGA 
GGGAAGCGAA GGGCACCCAA ACCACCTACA CCCCTACCAG ACTTCCAGGA TGAAGACAAT 
AGTGAAGATC CAGCCCCACT CCAGAAATCC TACTCTCTGT CTCCTACCAG TAGAAGGAAG 
AGCATACCAG AGGAGGAAAA TCACCTCTAT GAAAATTTGG GTGCCAATCG AGCCAACATA 
GCCCCTCAGA AGCCAGCTAG GAGGAGACAA CGGAACAGGA CCACAGGTTT TGAGGACTTG 
CCGTCTCGCG TCAAGCGTAT GGCCCCAAAG ACTCCAGAGA AACCAACCCC TGGCGACACA 
GGAGACGATG TCATCGACGG AAACGGTGCT AAGCAACTGG TGTTTGATGA CATCCATCTC 
AGTTCCTCCC CTCCTCTGAA GTCTGCTCTC AGAAGCACAC AATCAGAACC ACTCAGGACT 
GATGCTGAAG GGAAAGTGGT TCCAAAGCGT CCAGTCAGAA TCATCGCTCC GGAAGATGCA 
GCTATTGATA CCCAATCGCA TTCCACCGTC CTCCGAAGTG ACTATACAAA CCTGGCTCTG 
GTTCCACAAG TGCATCTGGA TGATACAATC CACAAGGACG CAGAAACCCA AGTCGACGAC 
AGTCCTCAAA GGGTAGAGCC ATCTCTCGCA GATGCTCGAT GCAGTTTGAA CCCGCTGAAC 
AGTTCATCCC TTGATCAGTC TAGCCAGCGC AATCTTCTCA ATGATGTCAG TGCTGTCCAT 
CGTCAAGCCT TGACTCAACT CTCAAGGACT ACACCAAGAA CACCACTAGA CTGGACAGAG 
TGTGAGGACT CGGTCTGGAA GGAGCAGGCC ACATCAGAGC CACAGTGTCG TTCAGCTGGG 
GCAGCATTCT TTGGTGTAAG ACATCCAACC AACCCTTCCT CTCCTGCAGG CCTACTGGTG 
TGTTGTGATC CTAACAAGGC AACTCAGGTG CTGCAAATGG CTGAAATGAC CAAATCAATG 
GCAACCCATC CCAACCTCAT CTCAGTGTCC CATGCTACCA AAGACACTAT CACCATCGAT 
CCTTCACTCT TTGAGCAAAG AAAAACCAGG AGCTCTCCTC TTACTGGTGG ATCCATTGAG 
ACAGCCTTGG TCCTCACGGA TGATTTACCG ACTGGGAATG TTGCAGAGTA TGTCTCTCAA 
AATACCGAGA AGCATGAGCA CTTACCAGAG GTCTATGAGC GAGATGTTGC TTTGATGCTG 
CTGCAATTGT GCCAGGGACT GTGTGAGCTT CAGTCACGCA GCATGTGCCT TGAGAGCCTA 
AAGCTTGACC ACCTCTTACT GACTGAGAGT GGCCTTCCAG GTGGAGGACA TCGTCTAGTC 
ATCAACCACT TGATGGGTGC GGAGGTGGTT TCTCCAGCGA GGACAGACGA GAAGGAGGTT 
GCATCCAAGC TGGAGCAGAA ATGCCATGAG TTTGATGTTG GAATCCTGAT CTTTGAGTTC 
CTTCATCAGC CAAACCCTTT CTCCACCAGG ACAAGTTTGA TCATCTCGGA TTACACTGCG 
GATGATCTTC CTGGTATCCC TGTCAAGTCT TCTCTCTCCG CTGATCTACG CACAATCGCT 
GCAAAACTCC TAGCCAAAAA TCCTCAAGAT AGACTCACCT GCAATCAGGC TGTATCCCTT 
CTCCAGTGCT TGCTCTGGGG CCCACCATCT ACGGTATACA CTGATAATGC AAACACAGCT 
GAGGCCCTTA ACCAAGAATT GGAAAGGTGG CTGGTGGCTG AGCAAGCAAA GATGGTAACT 
AAAGTTGCCC TGTATTCCTT GTTGGGGCGA GCATCTTCTG AAAACAAGTC AGGACTTACT 
CTCTCAGACC AACTGCAGTG TCAGTATCTA TCTGATTCGA CAGTGGATAC TTGCAAGGAG 
GCAATGAGGA TGTTGCATGG AAAAAATGAG AGTGAAACAG TAAATGTCAT TGATTCTGAC 
CTAGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011662588.1
CDS	212..5203
Translation	MLEDLKATTAACSAFEQHRFKSHLCVHCFKPKLAHVEKVDKKMDTTKILALDNNINNVQENDGKAAKQQKYDIPKVIIRNQNNLQKDVKGRGERQAERNNRKRGKERTKESLPHNAKRDVTSKATKNALTFNIPCEAECPSGLDNTESSRNESTQAGIKSSESCSENGSININVGQGQETNGNIYPDNNPSDSELELSGSYSEQYRRALRPVQKAGNPSKTRGRSRHDPELPEFVKVAKNLRHIQSMGNTSSAEVHSSNNNNHSVIETEEMLPNLPDLPNVVGSDASVEELQKLVDSKSNLPMNFVMNSQSTLTGHETLPKSVGKSRPSIKPLTKHIRQTSDPEVFMPSTNKKIVVNVSTSTGENMKDVKTENVVVRNSQLSSDVYFGKPPVTSPQKHAGDDEQQDLTSRYGHIADCVTIISQSGEVSHRYEIDKGDSGSAEMDNGSGSQSPTVRTKPYRVVDLNDNQAKKSSSNGTSSNSGRMSHELDEGLGLIQQEDGGASTSMDPSSRYSRRNVALVDGIYTASQLNTDSGINDSMESSDSSDAPKHETPPAQSSKRNTPPYPIILHDEQPPDNRKNKRNSGQYKVPIFIMPKAFNSSECDGTKDSNGGEVDAQVLQGLNQSVDYLQPKEKSSEKQVTAPSREETAKNESSQNLLESKRVSMSDETLRETRRPSRSRSKTHPVLEEELSNGSSGKDPDIPDFGQTRLKRVAPRPPSMELTEQNFGRERDDRLDSLPHRSPPPPPQNKKEKKKSIDRSSSDLSDDNDRSSYPQASNTDKLKVETRISGSSGRGDEVRREMSPTRHRDMSPLRNQHESRKATPVKKESNFRARLREFSPTGRRELSPVSRGKQKENQPSAPPMRRVASPPPPLNRPQPLNLPNTSATQDAPAVTTKSKRSKLPWKRKKKRNDSSSPEREFNSEEVEKWLTSSFCAPAAPPAVNDSHPRNSLERLEFINAYRGQPTVAIKQASQATTKAASPATPGTNGTTPTATTPTTATNHNRNSLVESEDDDVTRSGGKRRAPKPPTPLPDFQDEDNSEDPAPLQKSYSLSPTSRRKSIPEEENHLYENLGANRANIAPQKPARRRQRNRTTGFEDLPSRVKRMAPKTPEKPTPGDTGDDVIDGNGAKQLVFDDIHLSSSPPLKSALRSTQSEPLRTDAEGKVVPKRPVRIIAPEDAAIDTQSHSTVLRSDYTNLALVPQVHLDDTIHKDAETQVDDSPQRVEPSLADARCSLNPLNSSSLDQSSQRNLLNDVSAVHRQALTQLSRTTPRTPLDWTECEDSVWKEQATSEPQCRSAGAAFFGVRHPTNPSSPAGLLVCCDPNKATQVLQMAEMTKSMATHPNLISVSHATKDTITIDPSLFEQRKTRSSPLTGGSIETALVLTDDLPTGNVAEYVSQNTEKHEHLPEVYERDVALMLLQLCQGLCELQSRSMCLESLKLDHLLLTESGLPGGGHRLVINHLMGAEVVSPARTDEKEVASKLEQKCHEFDVGILIFEFLHQPNPFSTRTSLIISDYTADDLPGIPVKSSLSADLRTIAAKLLAKNPQDRLTCNQAVSLLQCLLWGPPSTVYTDNANTAEALNQELERWLVAEQAKMVTKVALYSLLGRASSENKSGLTLSDQLQCQYLSDSTVDTCKEAMRMLHGKNESETVNVIDSDLDL

Target ORF information:

RefSeq Version	XM_011664286.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus pseudopodium-enriched atypical kinase 1 (LOC752284), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011664286.1

ORF Insert Sequence:

ATGTTGGAAG ATCTGAAAGC TACCACTGCA GCCTGTTCAG CTTTTGAACA GCATCGCTTC 
AAAAGCCACC TCTGTGTTCA CTGCTTCAAG CCCAAGTTGG CTCATGTAGA GAAAGTCGAC 
AAGAAGATGG ATACCACAAA GATTCTCGCC CTTGACAACA ACATCAACAA TGTGCAAGAG 
AATGATGGGA AGGCTGCGAA ACAGCAGAAG TATGACATTC CCAAAGTCAT CATTCGCAAC 
CAGAATAACC TCCAGAAGGA TGTGAAAGGG CGGGGCGAGA GACAGGCTGA AAGGAATAAC 
AGGAAGAGGG GTAAAGAGAG AACTAAAGAA TCGTTACCCC ACAATGCAAA GCGTGATGTG 
ACGAGTAAAG CCACGAAGAA TGCCTTGACC TTTAACATCC CTTGTGAAGC AGAATGCCCG 
TCTGGTTTGG ACAACACGGA ATCATCTAGA AATGAATCGA CACAAGCTGG GATAAAGAGC 
TCCGAATCAT GTTCAGAGAA TGGAAGCATC AACATCAATG TTGGACAGGG ACAGGAGACC 
AATGGCAATA TCTACCCGGA TAACAACCCA TCAGACAGCG AGCTAGAGCT GAGTGGTAGT 
TACAGTGAGC AGTATAGGCG AGCATTGCGC CCCGTACAGA AGGCAGGAAA CCCGTCTAAG 
ACCAGAGGCA GGAGCCGCCA TGATCCAGAG CTCCCAGAGT TTGTGAAAGT CGCCAAAAAT 
CTTAGACACA TTCAGAGCAT GGGGAATACT TCTTCAGCAG AGGTTCATAG CAGCAATAAC 
AACAACCATT CTGTTATCGA GACAGAAGAG ATGCTCCCAA ATCTGCCTGA CTTGCCAAAC 
GTGGTTGGAA GTGATGCCAG TGTAGAGGAG CTACAGAAAT TAGTGGATTC CAAATCCAAT 
CTGCCTATGA ATTTTGTCAT GAACTCTCAG TCTACGCTGA CAGGTCACGA GACTCTCCCA 
AAGTCCGTAG GAAAGTCAAG GCCATCTATA AAGCCACTCA CAAAACACAT AAGACAGACT 
TCCGATCCGG AGGTTTTCAT GCCATCTACG AACAAAAAAA TTGTGGTAAA CGTCAGCACT 
TCGACAGGAG AAAACATGAA AGATGTGAAG ACTGAGAATG TAGTGGTGAG AAACAGCCAG 
CTCAGTTCAG ATGTTTATTT TGGCAAGCCT CCGGTCACGT CTCCCCAGAA ACATGCAGGC 
GACGATGAAC AACAAGATTT GACATCCCGA TATGGACATA TTGCAGATTG TGTCACAATT 
ATTTCACAGA GTGGGGAGGT ATCTCATCGC TATGAGATAG ACAAAGGAGA CAGTGGTAGT 
GCTGAGATGG ATAATGGTTC AGGTTCCCAG TCTCCTACAG TGAGAACAAA ACCCTACAGG 
GTTGTAGATT TGAATGATAA TCAGGCGAAA AAGTCAAGCA GTAATGGTAC TTCATCAAAT 
AGTGGTAGGA TGTCTCATGA ACTAGATGAA GGTCTTGGAT TGATCCAGCA GGAGGATGGT 
GGAGCTAGCA CCTCAATGGA TCCATCAAGT CGCTACTCAA GGAGAAATGT TGCTCTTGTT 
GATGGCATCT ATACTGCATC TCAGTTGAAC ACAGACTCTG GTATCAACGA TTCCATGGAA 
TCCAGTGATT CTAGTGATGC ACCAAAGCAC GAAACACCGC CTGCACAGAG CAGTAAAAGA 
AATACGCCAC CATACCCTAT CATTCTTCAT GATGAACAGC CTCCAGATAA TAGAAAAAAT 
AAACGAAATT CAGGACAGTA TAAGGTTCCA ATATTCATTA TGCCAAAGGC GTTCAACAGC 
TCAGAGTGCG ATGGTACCAA AGATAGTAAT GGTGGTGAGG TAGATGCTCA AGTACTGCAG 
GGTCTTAATC AAAGCGTTGA TTATCTCCAG CCTAAAGAGA AATCCAGTGA AAAGCAAGTT 
ACCGCTCCAA GCAGAGAGGA AACTGCTAAG AATGAAAGTT CACAGAATCT ACTGGAGTCA 
AAGAGAGTGT CAATGAGTGA TGAGACATTA AGAGAAACCA GGAGACCATC TCGATCAAGG 
TCAAAAACTC ATCCAGTCTT GGAGGAGGAA TTGTCAAATG GTTCGAGTGG GAAAGACCCA 
GATATACCAG ATTTTGGTCA GACTAGGCTT AAGAGGGTGG CTCCAAGGCC CCCATCGATG 
GAACTCACTG AGCAGAACTT TGGTAGGGAG AGGGATGACA GGCTGGATAG CTTGCCCCAT 
CGTTCCCCGC CCCCTCCCCC TCAAAACAAA AAAGAAAAGA AAAAATCAAT AGACAGAAGT 
TCCTCAGACC TATCGGATGA CAATGACAGA TCATCATATC CGCAGGCATC CAATACTGAC 
AAACTTAAGG TAGAAACTCG CATCAGTGGG AGCTCGGGAA GGGGTGATGA AGTAAGAAGA 
GAGATGTCCC CCACGAGACA CAGAGACATG TCTCCTCTTA GAAATCAACA TGAATCTAGA 
AAGGCGACTC CAGTCAAGAA AGAGAGTAAT TTTCGAGCGA GGCTCCGGGA GTTTTCTCCT 
ACCGGACGAA GGGAGCTGTC ACCAGTGAGC AGAGGGAAAC AGAAGGAGAA TCAACCCAGT 
GCCCCACCTA TGAGACGAGT GGCCTCTCCT CCACCTCCTC TTAACAGGCC CCAGCCACTT 
AACCTCCCTA ACACATCGGC CACACAGGAT GCACCTGCTG TTACCACAAA GTCTAAACGA 
TCCAAGTTGC CATGGAAGAG GAAGAAGAAG CGAAATGATT CTAGCAGCCC GGAGAGGGAA 
TTCAACTCGG AGGAGGTGGA GAAATGGCTT ACGAGTTCAT TCTGTGCTCC TGCTGCTCCG 
CCAGCAGTCA ATGACTCCCA TCCAAGGAAC TCGCTGGAGC GCCTGGAATT CATCAACGCA 
TACAGGGGGC AACCAACGGT GGCAATCAAA CAGGCATCCC AAGCTACTAC CAAAGCTGCA 
TCTCCTGCCA CACCCGGTAC CAATGGGACC ACACCCACAG CCACCACACC CACCACCGCA 
ACCAATCACA ACAGGAATAG CCTCGTGGAG AGCGAAGACG ATGATGTAAC CAGAAGTGGA 
GGGAAGCGAA GGGCACCCAA ACCACCTACA CCCCTACCAG ACTTCCAGGA TGAAGACAAT 
AGTGAAGATC CAGCCCCACT CCAGAAATCC TACTCTCTGT CTCCTACCAG TAGAAGGAAG 
AGCATACCAG AGGAGGAAAA TCACCTCTAT GAAAATTTGG GTGCCAATCG AGCCAACATA 
GCCCCTCAGA AGCCAGCTAG GAGGAGACAA CGGAACAGGA CCACAGGTTT TGAGGACTTG 
CCGTCTCGCG TCAAGCGTAT GGCCCCAAAG ACTCCAGAGA AACCAACCCC TGGCGACACA 
GGAGACGATG TCATCGACGG AAACGGTGCT AAGCAACTGG TGTTTGATGA CATCCATCTC 
AGTTCCTCCC CTCCTCTGAA GTCTGCTCTC AGAAGCACAC AATCAGAACC ACTCAGGACT 
GATGCTGAAG GGAAAGTGGT TCCAAAGCGT CCAGTCAGAA TCATCGCTCC GGAAGATGCA 
GCTATTGATA CCCAATCGCA TTCCACCGTC CTCCGAAGTG ACTATACAAA CCTGGCTCTG 
GTTCCACAAG TGCATCTGGA TGATACAATC CACAAGGACG CAGAAACCCA AGTCGACGAC 
AGTCCTCAAA GGGTAGAGCC ATCTCTCGCA GATGCTCGAT GCAGTTTGAA CCCGCTGAAC 
AGTTCATCCC TTGATCAGTC TAGCCAGCGC AATCTTCTCA ATGATGTCAG TGCTGTCCAT 
CGTCAAGCCT TGACTCAACT CTCAAGGACT ACACCAAGAA CACCACTAGA CTGGACAGAG 
TGTGAGGACT CGGTCTGGAA GGAGCAGGCC ACATCAGAGC CACAGTGTCG TTCAGCTGGG 
GCAGCATTCT TTGGTGTAAG ACATCCAACC AACCCTTCCT CTCCTGCAGG CCTACTGGTG 
TGTTGTGATC CTAACAAGGC AACTCAGGTG CTGCAAATGG CTGAAATGAC CAAATCAATG 
GCAACCCATC CCAACCTCAT CTCAGTGTCC CATGCTACCA AAGACACTAT CACCATCGAT 
CCTTCACTCT TTGAGCAAAG AAAAACCAGG AGCTCTCCTC TTACTGGTGG ATCCATTGAG 
ACAGCCTTGG TCCTCACGGA TGATTTACCG ACTGGGAATG TTGCAGAGTA TGTCTCTCAA 
AATACCGAGA AGCATGAGCA CTTACCAGAG GTCTATGAGC GAGATGTTGC TTTGATGCTG 
CTGCAATTGT GCCAGGGACT GTGTGAGCTT CAGTCACGCA GCATGTGCCT TGAGAGCCTA 
AAGCTTGACC ACCTCTTACT GACTGAGAGT GGCCTTCCAG GTGGAGGACA TCGTCTAGTC 
ATCAACCACT TGATGGGTGC GGAGGTGGTT TCTCCAGCGA GGACAGACGA GAAGGAGGTT 
GCATCCAAGC TGGAGCAGAA ATGCCATGAG TTTGATGTTG GAATCCTGAT CTTTGAGTTC 
CTTCATCAGC CAAACCCTTT CTCCACCAGG ACAAGTTTGA TCATCTCGGA TTACACTGCG 
GATGATCTTC CTGGTATCCC TGTCAAGTCT TCTCTCTCCG CTGATCTACG CACAATCGCT 
GCAAAACTCC TAGCCAAAAA TCCTCAAGAT AGACTCACCT GCAATCAGGC TGTATCCCTT 
CTCCAGTGCT TGCTCTGGGG CCCACCATCT ACGGTATACA CTGATAATGC AAACACAGCT 
GAGGCCCTTA ACCAAGAATT GGAAAGGTGG CTGGTGGCTG AGCAAGCAAA GATGGTAACT 
AAAGTTGCCC TGTATTCCTT GTTGGGGCGA GCATCTTCTG AAAACAAGTC AGGACTTACT 
CTCTCAGACC AACTGCAGTG TCAGTATCTA TCTGATTCGA CAGTGGATAC TTGCAAGGAG 
GCAATGAGGA TGTTGCATGG AAAAAATGAG AGTGAAACAG TAAATGTCAT TGATTCTGAC 
CTAGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC752284 ( XM_031000296.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030856156.1 Strongylocentrotus purpuratus inactive tyrosine-protein kinase PEAK1 (LOC752284), mRNA.

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CloneID	OSd66259
Clone ID Related Accession (Same CDS sequence)	XM_031000296.1
Accession Version	XM_031000296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	inactive tyrosine-protein kinase PEAK1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAAG ATCTGAAAGC TACCACTGCA GCCTGTTCAG CTTTTGAACA GCATCGCTTC 
AAAAGCCACC TCTGTGTTCA CTGCTTCAAG CCCAAGTTGG CTCATGTAGA GAAAGTCGAC 
AAGAAGATGG ATACCACAAA GATTCTCGCC CTTGACAACA ACATCAACAA TGTGCAAGAG 
CATGATGGGA AGGCTGCGAA GCAGCAGAAG TATGACATTC CCAAAGTCAT CATTCGCAAC 
CAGAATAACC TCCAGAAGGA TGTGAAAGGG CGGGGCGAGA GACAGGCTGA AAGGAATAAC 
AGGAAGAGGG GTAAAGAGAG AACTAAAGAA TCGTTACCCC ACAATGCAAA GCGTGATGCG 
ACGAGTAAAG CCACAAAGAA TGCCTTGACC TTCAACATCC CTTGTGAAGC AGAATGCCCG 
TCTGGTTTGG ACAACACGGT ATCATCTAGA AATGAATCGA CACAAGCTGG GATAAAGAGC 
TCTGAATCAT GTTCAGAGAA TGGAAGCATC AACATCAATG TTGGACAGGG ACAGGAGACC 
AATGGCAATA TCTACCCGGA TAACAACCCA TCAGACAGCG AGCTAGAGCT GAGTGGTAGT 
TACAGTGAGC AGTATAGGCG AGCATTGCGC CCCGTACAGA AGGCAGGAAA CCCGTCTAAG 
ACCAGAGGCA GGAGCCGCCA TGATCCAGAG CTCCCAGAGT TTGTGAAAGT CGCCAAAAAT 
CTTAGACACA TTCAGAGCAT GGGGAATACT TCTTCAGCAG AGGTTCATAG CAGCAATAAC 
AACAACCATT CTGTTATCGA GACAGAAGAG ATGCTCCCAA ATCTGCCTGA CTTGCCAAAC 
GTGGTTGGAA GTGATGCCAG TGTAGAGGAG CTACAGAAAT TAGTGGATTC CAAATCCAAT 
CTGCCTATGA ATTTTGTCAT GAACTCTCAG TCTACGCTGA CAGGTCACGA GACTCTCCCA 
AAGTCCGTAG GAAAGTCAAG GCCATCTATA AAGCCACTCA CAAAACACAT AAGACAGACT 
TCCGATCCGG AGGTTTTCAT GCCATCTACG AACAAAAAAA TTGTGGTAAA CGTCAGCACT 
TCGACAGGAG AAAACATGAA AGATGTGAAG ACTGAGAATG TAGTGGTGAG AAACAGCCAG 
CTCAGTTCAG ATGTTTATTT TGGCAAGCCT CCGGTCACGT CTCCCCAGAA ACATGCAGGC 
GACGATGAAC AACAAGATTT GACATCCCGA TATGGACATA TTGCAGATTG TGTCACAATT 
ATTTCACAGA GTGGGGAGGT ATCTCATCGC TATGAGATAG ACAAAGGAGA CAGTGGTAGT 
GCTGAGATGG ATAATGGTTC AGGTTCCCAG TCTCCTACAG TGAGAACAAA ACCCTACAGG 
GTTGTAGATT TGAATGATAA TCAGGCGAAA AAGTCAAGCA GTAATGGTAC TTCATCAAAT 
AGTGGTAGGA TGTCTCATGA ACTAGATGAA GGTCTTGGAT TGATCCAGCA GGAGGATGGT 
GGAGCTAGCA CCTCAATGGA TCCATCAAGT CGCTACTCAA GGAGAAATGT TGCTCTTGTT 
GATGGCATCT ATACTGCATC TCAGTTGAAC ACAGACTCTG GTATCAACGA TTCCATGGAA 
TCCAGTGATT CTAGTGATGC ACCAAAGCAC GAAACACCGC CTGCACAGAG CAGTAAAAGA 
AATACGCCAC CATACCCTAT CATTCTTCAT GATGAACAGC CTCCAGATAA TAGAAAAAAT 
AAACGAAATT CAGGACAGTA TAAGGTTCCA ATATTCATTA TGCCAAAGGC GTTCAACAGC 
TCAGAGTGCG ATGGTACCAA AGATAGTAAT GGTGGTGAGG TAGATGCTCA AGTACTGCAG 
GGTCTTAATC AAAGCGTTGA TTATCTCCAG CCTAAAGAGA AATCAAGTGA AAAGCAAGTT 
ACCGCTCCAA GCAGAGAGGA AACTGCTAAG AATGAAAGTT CACAGAATCT ACTGGAGTCA 
AAGAGAGTGT CAATGAGTGA TGAGACATTA AGAGAAACCA GGAGACCATC TCGATCAAGG 
TCAAAAACTC ATCCAGTCTT GGAGGAGGAA TTGTCAAATG GTTCGAGTGG GAAAGACCCA 
GATATACCAG ATTTTGGTCA GACTAGGCTT AAGAGGGTGG CTCCAAGGCC CCCATCGATG 
GAACTCACTG AGCAGAACTT TGGTAGGGAG AGGGATGACA GGCTGGATAG CTTGCCCCAT 
CGTTCCCCGC CCCCTCCCCC TCAAAACAAA AAAGAAAAGA AAAAATCAAT AGACAGAAGT 
TCCTCAGACC TATCGGATGA CAATGACAGA TCATCATATC CGCAGGCATC CAATACTGAC 
AAACTTAAGG TAGAAACTCG CATTAGTGGG AGCTCGGGAA GGGGTGATGA AGTAAGAAGA 
GAGATGTCCC CCACGAGACA CAGAGACATG TCTCCTCTTA GAAATCAACA TGAATCTAGA 
AAGGCAACTC CAGTCAAGAA AGAGAGTAAT TTTCGAGCGA GGCTCCGGGA GTTTTCTCCT 
ACCGGACGAA GGGAGCTGTC ACCGGTGAGC AGAGGGAAAC AGAAGGAGAA TCAACCCAGT 
GCCCCACCTA TGAGACGAGT GGCCTCTCCT CCACCTCCTC TTAACAGGCC CCAGCAACTT 
AACCTCCCTA ACACATCGGC CACACAGGAT GCACCTGCTG TTACCACAAA GTCTAAACGA 
TCCAAGTTGC CATGGAAGAG GAAGAAGAAG CGAAATGATT CTGGCAGCCC GGAGAGGGAA 
TTCAACTCGG AGGAGGTGGA GAAATGGCTT ACGAGTTCAT TCTGTGCTCC TGCTGCTCCG 
CCAGCAGTCA ATGACTCCCA TCCAAGGAAC TCGCTGGAGC GCCTGGAAGT CATCAACGCA 
TACAGGGGGC AACCAACGGT GGCTATCAAA CAGGCATCCC AAGCTACTAC CAAAGCTGCA 
TCTCCTGCCA CACCCGGTAC CAATGGGACC ACACCCACAG CCACCACACC CACCACCGCA 
ACCAATCACA ACAGGAATAG CCTCGTGGAG AGCGAAGACG ATGATGTAAC CAGAAGTGGA 
GGGAAGCGAA GGGCACCCAA ACCACCTACA CCCCTACCAG ACTTCCAGGA TGAAGACAAT 
AGTGAAGATC CAGCCCCACT CCAGAAATCC TACTCTCTGT CTCCTACCAG TAGAAGGAAG 
AGCATACCAG AGGAGGAAAA TCACCTCTAT GAAAATTTGG GTGCCAATCG AGCCAACATA 
GCCCCTCAGA AGCCAGCTAG GAGGAGACAA CGGAACAGGA CCACAGGTTT TGAGGACTTG 
CCGTCTCGCG TCAAGCGTAT GGCCCCAAAG ACTCCAGAGA AACCAACCCC TGGCGACACA 
GGAGACGATG TCATCGACGG AAACGGTGCT AAGCAACTGG TGTTTGATGA CATCCATCTC 
AGTTCCTCCC CTCCTCTGAA GTCTGCTCTC AGAAGCACAC AATCAGAACC ACTCAGGACT 
GATGCTGAAG GGAAAGTGGT TCCAAAGCGT CCAGTCAGAA TCATCGCTCC GGAAGATGCA 
GCTATTGATA CCCAATCGCA TTCCACCGTC CTCCGAAGTG ACTATACAAA CCTGGCTCTG 
GTTCCACAAG TGCATCTGGA TGATACAATC CACAAGGACG CAGAAACCCA AGTCGACGAC 
AGTCCTCAAA GGGTAGAGCC ATCTCTCGCA GATGCTCGAT GCAGTTTGAA CCCGCTGAAC 
AGTTCATCCC TTGATCAGTC TAGCCAGCGC AATCTTCTCA ATGATGTCAG TGCTGTCCAT 
CGTCAAGCCT TGACTCAACT CTCAAGGACT ACACCAAGAA CACCACTAGA CTGGACAGAG 
TGTGAGGACT CGGTCTGGAA GGAGCAGGCC ACATCAGAGC CACAGTGTCG TTCAGCTGGG 
GCAGCATTCT TTGGTGTAAG ACATCCAACC AACCCTTCCT CTCCTGCAGG CCTACTGGTG 
TGTTGTGATC CTAACAAGGC AACTCAGGTG CTGCAAATGG CTGAAATGAC CAAATCAATG 
GCAACCCATC CCAACCTCAT CTCAGTGTCC CATGCTACCA AAGACACTAT CACCATCGAT 
CCTTCACTCT TTGAGCAAAG AAAAACCAGG AGCTCTCCTC TTACTGGTGG ATCCATTGAG 
ACAGCCTTGG TCCTCACGGA TGATTTACCG ACTGGGAATG TTGCAGAGTA TGTCTCTCAA 
AATACCGAGA AGCATGAGCA CTTACCAGAG GTCTATGAGC GAGATGTTGC TTTGATGCTG 
CTGCAATTGT GCCAGGGACT GTGTGAGCTT CAGTCACGCA GCATGTGCCT TGAGAGCCTA 
AAGCTTGACC ACCTCTTACT GACTGAGAGT GGCCTTCCAG GTGGAGGACA TCGTCTAGTC 
ATCAACCACT TGATGGGTGC GGAGGTGGTT TCTCCAGCGA GGACAGACGA GAAGGAGGTT 
GCATCCAAGC TGGAGCAGAA ATGCCATGAG TTTGATGTTG GAATCCTGAT CTTTGAGTTC 
CTTCATCAGC CAAACCCTTT CTCCACCAGG ACAAGTTTGA TCATCTCGGA TTACACTGCG 
GATGATCTTC CTGGTATCCC TGTCAAGTCT TCTCTCTCCG CTGATCTACG CACAATCGCT 
GCAAAACTCC TAGCCAAAAA TCCTCAAGAT AGACTCACCT GCAATCAGGC TGTATCCCTT 
CTCCAGTGCT TGCTCTGGGG CCCACCATCT ACGGTATACA CTGATAATGC AAACACAGCT 
GAGGCCCTTA ACCAAGAATT GGAAAGGTGG CTGGTGGCTG AGCAAGCAAA GATGGTAACT 
AAAGTTGCCC TGTATTCCTT GTTGGGGCGA GCATCTTCTG AAAACAAGTC AGGACTTACT 
CTCTCAGACC AACTGCAGTG TCAGTATCTA TCTGATTCGA CAGTGGATAC TTGCAAGGAG 
GCAATGAGGA TGTTGCATGG AAAAAATGAG AGTGAAACAG TAAATGTCAT TGATTCTGAC 
CTAGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030856156.1
CDS	235..5226
Translation	MLEDLKATTAACSAFEQHRFKSHLCVHCFKPKLAHVEKVDKKMDTTKILALDNNINNVQEHDGKAAKQQKYDIPKVIIRNQNNLQKDVKGRGERQAERNNRKRGKERTKESLPHNAKRDATSKATKNALTFNIPCEAECPSGLDNTVSSRNESTQAGIKSSESCSENGSININVGQGQETNGNIYPDNNPSDSELELSGSYSEQYRRALRPVQKAGNPSKTRGRSRHDPELPEFVKVAKNLRHIQSMGNTSSAEVHSSNNNNHSVIETEEMLPNLPDLPNVVGSDASVEELQKLVDSKSNLPMNFVMNSQSTLTGHETLPKSVGKSRPSIKPLTKHIRQTSDPEVFMPSTNKKIVVNVSTSTGENMKDVKTENVVVRNSQLSSDVYFGKPPVTSPQKHAGDDEQQDLTSRYGHIADCVTIISQSGEVSHRYEIDKGDSGSAEMDNGSGSQSPTVRTKPYRVVDLNDNQAKKSSSNGTSSNSGRMSHELDEGLGLIQQEDGGASTSMDPSSRYSRRNVALVDGIYTASQLNTDSGINDSMESSDSSDAPKHETPPAQSSKRNTPPYPIILHDEQPPDNRKNKRNSGQYKVPIFIMPKAFNSSECDGTKDSNGGEVDAQVLQGLNQSVDYLQPKEKSSEKQVTAPSREETAKNESSQNLLESKRVSMSDETLRETRRPSRSRSKTHPVLEEELSNGSSGKDPDIPDFGQTRLKRVAPRPPSMELTEQNFGRERDDRLDSLPHRSPPPPPQNKKEKKKSIDRSSSDLSDDNDRSSYPQASNTDKLKVETRISGSSGRGDEVRREMSPTRHRDMSPLRNQHESRKATPVKKESNFRARLREFSPTGRRELSPVSRGKQKENQPSAPPMRRVASPPPPLNRPQQLNLPNTSATQDAPAVTTKSKRSKLPWKRKKKRNDSGSPEREFNSEEVEKWLTSSFCAPAAPPAVNDSHPRNSLERLEVINAYRGQPTVAIKQASQATTKAASPATPGTNGTTPTATTPTTATNHNRNSLVESEDDDVTRSGGKRRAPKPPTPLPDFQDEDNSEDPAPLQKSYSLSPTSRRKSIPEEENHLYENLGANRANIAPQKPARRRQRNRTTGFEDLPSRVKRMAPKTPEKPTPGDTGDDVIDGNGAKQLVFDDIHLSSSPPLKSALRSTQSEPLRTDAEGKVVPKRPVRIIAPEDAAIDTQSHSTVLRSDYTNLALVPQVHLDDTIHKDAETQVDDSPQRVEPSLADARCSLNPLNSSSLDQSSQRNLLNDVSAVHRQALTQLSRTTPRTPLDWTECEDSVWKEQATSEPQCRSAGAAFFGVRHPTNPSSPAGLLVCCDPNKATQVLQMAEMTKSMATHPNLISVSHATKDTITIDPSLFEQRKTRSSPLTGGSIETALVLTDDLPTGNVAEYVSQNTEKHEHLPEVYERDVALMLLQLCQGLCELQSRSMCLESLKLDHLLLTESGLPGGGHRLVINHLMGAEVVSPARTDEKEVASKLEQKCHEFDVGILIFEFLHQPNPFSTRTSLIISDYTADDLPGIPVKSSLSADLRTIAAKLLAKNPQDRLTCNQAVSLLQCLLWGPPSTVYTDNANTAEALNQELERWLVAEQAKMVTKVALYSLLGRASSENKSGLTLSDQLQCQYLSDSTVDTCKEAMRMLHGKNESETVNVIDSDLDL

Target ORF information:

RefSeq Version	XM_031000296.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus inactive tyrosine-protein kinase PEAK1 (LOC752284), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031000296.1

ORF Insert Sequence:

ATGTTGGAAG ATCTGAAAGC TACCACTGCA GCCTGTTCAG CTTTTGAACA GCATCGCTTC 
AAAAGCCACC TCTGTGTTCA CTGCTTCAAG CCCAAGTTGG CTCATGTAGA GAAAGTCGAC 
AAGAAGATGG ATACCACAAA GATTCTCGCC CTTGACAACA ACATCAACAA TGTGCAAGAG 
CATGATGGGA AGGCTGCGAA GCAGCAGAAG TATGACATTC CCAAAGTCAT CATTCGCAAC 
CAGAATAACC TCCAGAAGGA TGTGAAAGGG CGGGGCGAGA GACAGGCTGA AAGGAATAAC 
AGGAAGAGGG GTAAAGAGAG AACTAAAGAA TCGTTACCCC ACAATGCAAA GCGTGATGCG 
ACGAGTAAAG CCACAAAGAA TGCCTTGACC TTCAACATCC CTTGTGAAGC AGAATGCCCG 
TCTGGTTTGG ACAACACGGT ATCATCTAGA AATGAATCGA CACAAGCTGG GATAAAGAGC 
TCTGAATCAT GTTCAGAGAA TGGAAGCATC AACATCAATG TTGGACAGGG ACAGGAGACC 
AATGGCAATA TCTACCCGGA TAACAACCCA TCAGACAGCG AGCTAGAGCT GAGTGGTAGT 
TACAGTGAGC AGTATAGGCG AGCATTGCGC CCCGTACAGA AGGCAGGAAA CCCGTCTAAG 
ACCAGAGGCA GGAGCCGCCA TGATCCAGAG CTCCCAGAGT TTGTGAAAGT CGCCAAAAAT 
CTTAGACACA TTCAGAGCAT GGGGAATACT TCTTCAGCAG AGGTTCATAG CAGCAATAAC 
AACAACCATT CTGTTATCGA GACAGAAGAG ATGCTCCCAA ATCTGCCTGA CTTGCCAAAC 
GTGGTTGGAA GTGATGCCAG TGTAGAGGAG CTACAGAAAT TAGTGGATTC CAAATCCAAT 
CTGCCTATGA ATTTTGTCAT GAACTCTCAG TCTACGCTGA CAGGTCACGA GACTCTCCCA 
AAGTCCGTAG GAAAGTCAAG GCCATCTATA AAGCCACTCA CAAAACACAT AAGACAGACT 
TCCGATCCGG AGGTTTTCAT GCCATCTACG AACAAAAAAA TTGTGGTAAA CGTCAGCACT 
TCGACAGGAG AAAACATGAA AGATGTGAAG ACTGAGAATG TAGTGGTGAG AAACAGCCAG 
CTCAGTTCAG ATGTTTATTT TGGCAAGCCT CCGGTCACGT CTCCCCAGAA ACATGCAGGC 
GACGATGAAC AACAAGATTT GACATCCCGA TATGGACATA TTGCAGATTG TGTCACAATT 
ATTTCACAGA GTGGGGAGGT ATCTCATCGC TATGAGATAG ACAAAGGAGA CAGTGGTAGT 
GCTGAGATGG ATAATGGTTC AGGTTCCCAG TCTCCTACAG TGAGAACAAA ACCCTACAGG 
GTTGTAGATT TGAATGATAA TCAGGCGAAA AAGTCAAGCA GTAATGGTAC TTCATCAAAT 
AGTGGTAGGA TGTCTCATGA ACTAGATGAA GGTCTTGGAT TGATCCAGCA GGAGGATGGT 
GGAGCTAGCA CCTCAATGGA TCCATCAAGT CGCTACTCAA GGAGAAATGT TGCTCTTGTT 
GATGGCATCT ATACTGCATC TCAGTTGAAC ACAGACTCTG GTATCAACGA TTCCATGGAA 
TCCAGTGATT CTAGTGATGC ACCAAAGCAC GAAACACCGC CTGCACAGAG CAGTAAAAGA 
AATACGCCAC CATACCCTAT CATTCTTCAT GATGAACAGC CTCCAGATAA TAGAAAAAAT 
AAACGAAATT CAGGACAGTA TAAGGTTCCA ATATTCATTA TGCCAAAGGC GTTCAACAGC 
TCAGAGTGCG ATGGTACCAA AGATAGTAAT GGTGGTGAGG TAGATGCTCA AGTACTGCAG 
GGTCTTAATC AAAGCGTTGA TTATCTCCAG CCTAAAGAGA AATCAAGTGA AAAGCAAGTT 
ACCGCTCCAA GCAGAGAGGA AACTGCTAAG AATGAAAGTT CACAGAATCT ACTGGAGTCA 
AAGAGAGTGT CAATGAGTGA TGAGACATTA AGAGAAACCA GGAGACCATC TCGATCAAGG 
TCAAAAACTC ATCCAGTCTT GGAGGAGGAA TTGTCAAATG GTTCGAGTGG GAAAGACCCA 
GATATACCAG ATTTTGGTCA GACTAGGCTT AAGAGGGTGG CTCCAAGGCC CCCATCGATG 
GAACTCACTG AGCAGAACTT TGGTAGGGAG AGGGATGACA GGCTGGATAG CTTGCCCCAT 
CGTTCCCCGC CCCCTCCCCC TCAAAACAAA AAAGAAAAGA AAAAATCAAT AGACAGAAGT 
TCCTCAGACC TATCGGATGA CAATGACAGA TCATCATATC CGCAGGCATC CAATACTGAC 
AAACTTAAGG TAGAAACTCG CATTAGTGGG AGCTCGGGAA GGGGTGATGA AGTAAGAAGA 
GAGATGTCCC CCACGAGACA CAGAGACATG TCTCCTCTTA GAAATCAACA TGAATCTAGA 
AAGGCAACTC CAGTCAAGAA AGAGAGTAAT TTTCGAGCGA GGCTCCGGGA GTTTTCTCCT 
ACCGGACGAA GGGAGCTGTC ACCGGTGAGC AGAGGGAAAC AGAAGGAGAA TCAACCCAGT 
GCCCCACCTA TGAGACGAGT GGCCTCTCCT CCACCTCCTC TTAACAGGCC CCAGCAACTT 
AACCTCCCTA ACACATCGGC CACACAGGAT GCACCTGCTG TTACCACAAA GTCTAAACGA 
TCCAAGTTGC CATGGAAGAG GAAGAAGAAG CGAAATGATT CTGGCAGCCC GGAGAGGGAA 
TTCAACTCGG AGGAGGTGGA GAAATGGCTT ACGAGTTCAT TCTGTGCTCC TGCTGCTCCG 
CCAGCAGTCA ATGACTCCCA TCCAAGGAAC TCGCTGGAGC GCCTGGAAGT CATCAACGCA 
TACAGGGGGC AACCAACGGT GGCTATCAAA CAGGCATCCC AAGCTACTAC CAAAGCTGCA 
TCTCCTGCCA CACCCGGTAC CAATGGGACC ACACCCACAG CCACCACACC CACCACCGCA 
ACCAATCACA ACAGGAATAG CCTCGTGGAG AGCGAAGACG ATGATGTAAC CAGAAGTGGA 
GGGAAGCGAA GGGCACCCAA ACCACCTACA CCCCTACCAG ACTTCCAGGA TGAAGACAAT 
AGTGAAGATC CAGCCCCACT CCAGAAATCC TACTCTCTGT CTCCTACCAG TAGAAGGAAG 
AGCATACCAG AGGAGGAAAA TCACCTCTAT GAAAATTTGG GTGCCAATCG AGCCAACATA 
GCCCCTCAGA AGCCAGCTAG GAGGAGACAA CGGAACAGGA CCACAGGTTT TGAGGACTTG 
CCGTCTCGCG TCAAGCGTAT GGCCCCAAAG ACTCCAGAGA AACCAACCCC TGGCGACACA 
GGAGACGATG TCATCGACGG AAACGGTGCT AAGCAACTGG TGTTTGATGA CATCCATCTC 
AGTTCCTCCC CTCCTCTGAA GTCTGCTCTC AGAAGCACAC AATCAGAACC ACTCAGGACT 
GATGCTGAAG GGAAAGTGGT TCCAAAGCGT CCAGTCAGAA TCATCGCTCC GGAAGATGCA 
GCTATTGATA CCCAATCGCA TTCCACCGTC CTCCGAAGTG ACTATACAAA CCTGGCTCTG 
GTTCCACAAG TGCATCTGGA TGATACAATC CACAAGGACG CAGAAACCCA AGTCGACGAC 
AGTCCTCAAA GGGTAGAGCC ATCTCTCGCA GATGCTCGAT GCAGTTTGAA CCCGCTGAAC 
AGTTCATCCC TTGATCAGTC TAGCCAGCGC AATCTTCTCA ATGATGTCAG TGCTGTCCAT 
CGTCAAGCCT TGACTCAACT CTCAAGGACT ACACCAAGAA CACCACTAGA CTGGACAGAG 
TGTGAGGACT CGGTCTGGAA GGAGCAGGCC ACATCAGAGC CACAGTGTCG TTCAGCTGGG 
GCAGCATTCT TTGGTGTAAG ACATCCAACC AACCCTTCCT CTCCTGCAGG CCTACTGGTG 
TGTTGTGATC CTAACAAGGC AACTCAGGTG CTGCAAATGG CTGAAATGAC CAAATCAATG 
GCAACCCATC CCAACCTCAT CTCAGTGTCC CATGCTACCA AAGACACTAT CACCATCGAT 
CCTTCACTCT TTGAGCAAAG AAAAACCAGG AGCTCTCCTC TTACTGGTGG ATCCATTGAG 
ACAGCCTTGG TCCTCACGGA TGATTTACCG ACTGGGAATG TTGCAGAGTA TGTCTCTCAA 
AATACCGAGA AGCATGAGCA CTTACCAGAG GTCTATGAGC GAGATGTTGC TTTGATGCTG 
CTGCAATTGT GCCAGGGACT GTGTGAGCTT CAGTCACGCA GCATGTGCCT TGAGAGCCTA 
AAGCTTGACC ACCTCTTACT GACTGAGAGT GGCCTTCCAG GTGGAGGACA TCGTCTAGTC 
ATCAACCACT TGATGGGTGC GGAGGTGGTT TCTCCAGCGA GGACAGACGA GAAGGAGGTT 
GCATCCAAGC TGGAGCAGAA ATGCCATGAG TTTGATGTTG GAATCCTGAT CTTTGAGTTC 
CTTCATCAGC CAAACCCTTT CTCCACCAGG ACAAGTTTGA TCATCTCGGA TTACACTGCG 
GATGATCTTC CTGGTATCCC TGTCAAGTCT TCTCTCTCCG CTGATCTACG CACAATCGCT 
GCAAAACTCC TAGCCAAAAA TCCTCAAGAT AGACTCACCT GCAATCAGGC TGTATCCCTT 
CTCCAGTGCT TGCTCTGGGG CCCACCATCT ACGGTATACA CTGATAATGC AAACACAGCT 
GAGGCCCTTA ACCAAGAATT GGAAAGGTGG CTGGTGGCTG AGCAAGCAAA GATGGTAACT 
AAAGTTGCCC TGTATTCCTT GTTGGGGCGA GCATCTTCTG AAAACAAGTC AGGACTTACT 
CTCTCAGACC AACTGCAGTG TCAGTATCTA TCTGATTCGA CAGTGGATAC TTGCAAGGAG 
GCAATGAGGA TGTTGCATGG AAAAAATGAG AGTGAAACAG TAAATGTCAT TGATTCTGAC 
CTAGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC752284 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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