LOC591288 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC591288
Entrez Gene ID	591288
Full Name	DNA topoisomerase 3-beta-1
Synonyms	top3b
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011684814.1	XP_011683116.1	DNA topoisomerase 3-beta-1
XM_030989368.1	XP_030845228.1	DNA topoisomerase 3-beta-1

Pathways from BioSystems
Gene Ontology

The following LOC591288 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC591288 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSd26887	XM_011684814.1 Latest version!	Strongylocentrotus purpuratus DNA topoisomerase 3-beta-1 (LOC591288), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSd55484	XM_030989368.1 Latest version!	Strongylocentrotus purpuratus DNA topoisomerase 3-beta-1 (LOC591288), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC591288 ( XM_011684814.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011683116.1 Strongylocentrotus purpuratus DNA topoisomerase 3-beta-1 (LOC591288), mRNA.

Price & Availability↑

CloneID	OSd26887
Clone ID Related Accession (Same CDS sequence)	XM_011684814.1
Accession Version	XM_011684814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	DNA topoisomerase 3-beta-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011995666.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTAAAT TTCTCGCTAT AGAGGGCAAG GGCTGTGACT ACCTTGTGCT ATGGCTCGAC 
TGTGACAAGG AAGGTGAAAA CATCTGCTTT GAGGTTATCA GCTGCGTGCA GGGGGTCATG 
ACGAGACCAG TCGGAAGGGA ACAGGTAATC TTCCGTGCTA AATTCAGCGC TATCACGGAC 
AAGGACATCA GACAGGCCAT GAACACCCTG GTAGAGCCTA ACGAGAACGA AGCCCTCTCT 
GTCGACGCAA GGCAGGAACT GGATCTCAGG ATTGGATGTG CCTTCACCCG CTTCCAGACC 
AAGTACTTCC AGGGTAAATA TGGTGATCTC GACAGTACCT TAATCTCCTA CGGCCCTTGC 
CAGACTCCAA CCCTAGGCTT CTGTGTGAAC AGACACGACA AGATCCAGTC CTTCAAGCCT 
GAACCGTTCT GGGTCATTCA GATACAGGTA ACTCACGAGA GTGGAAGAAA GCTAAACCTT 
GACTGGGATC GAGTGAGAGT CTTTGACAGA GAAGTGGCTC AGATGTTTCT CAATATCATC 
AAAACACAGA AAGATGCAAG GATTACATCG GTGTCGCAGA AGGAGAAAGC CAAACAGCGC 
CCCCAGGCTC TCAATACGGT AGAGTTGATG CGTGTGGCGA GTTCATCGCT TGGTATGAGT 
CCCCACCATG CCATGCAGAT AGCCGAGAGG CTCTACATAC AGGGATACAT CAGCTACCCA 
AGAACAGAGA CAACAAGCTA TCCTGAGAGC TTTGACCTTA AGGGTACCTT GCGGCAACAG 
ATGTCGGCGC CCTGGAGGTC CGAAGTAAAG GTCCTCCTTG ACGGAGAATT CACCAAGCCC 
AGGAAAGGAA CCGACGTAGG AGATCATCCT CCTATCACGC CGATGAGAGC CGCTAACGAG 
AGTGAGGTGG GAGGAGAGGC GTGGAGGCTG TATGAGTATA TTGCCAGGCA CTTCATCGCT 
ACAGTGAGCC CCGACTGTCG CTACCTGCTC ACCACCATCC GTCTGATGAT CGGAGAGGAG 
GGCTTCAGCT GCACCGGTAA GACCCTGATC AGCGATGGCT TCACTGCAGT CATGCCCTGG 
CAGGCCATCC AAGATGAAGA GAACCTTCCC AACGTTACCA AGGGGGATAT GTGCTCCATT 
GAAGAGGCCA AGCTGAGTGA ACGCCAGACC AGCCCTCCCG ACTATCTGAC CGAAAGTGAA 
CTGATCTCCC TCATGGAGAA GCACGGTGTA GGTACAGACG CCAGCATACC CGTTCACATC 
GAGAACATCT GTCAGAGGAA CTATGTGAAG GTGGAGAGTG GGCGTCGTCT AGTACCCACA 
AGGCTTGGCA TTGTTCTGGT CCATGGATAT CAAAAGATTG ATCCAGACCT GTCTCTGCCC 
ACCATGAGAT CTGCGGTGGA GGAGCAGCTA AACCTGATCG CGCATGGCCA GGCCGACTTC 
AACTCTGTTC TGATACACAC CATAGACATC TTCACCAGGA AGTTCCTCTA CTTTGTAGAT 
CACATACAGG CCATGGATGA TCTCTTTGAG GTCTCCTTCT CGCCTCTGGC TGCCACCGGG 
AAACCCATGT CAAGATGCGG AAAGTGCCAT CGCTTCATGA AGTACATCTC GGCCAAGCCG 
ATGCGTCTTC ACTGCCCTAC CTGCGAAGAG ACGTACAGCC TCCCTCAGAA CGGTTCCATC 
AAGCTCTACA AAGAGATCAG GTGCCCCCTG GATGATTTTG AGCTGGTCCT GTGGTCTTCT 
GGGCCAAAGG GAAAGAGCTA CCCAGTGTGC CCCTACTGCT TCAGCCACCC CCCTTTCACG 
GAGATGAAGA AGGGACAGGG GTGCAACGGA TGCACCCATC CCACCTGTGC CCACTCCTTA 
ACCATGCTGG GCGTGGCTCA GTGTATCGAA TGCGATGAAG GGGTAATGGT GCTGGACCCC 
TCCTCGGCAC CCAAGTGGAG ACTTGCCTGC AACAAATGCA ACATCATCAT GAACATCTTC 
GAGGATGCTC ACAAGGTGAC CTTGTCGGGT CACCTGTGCG AAAAGTGTGA TGCTTCCATT 
CTAAATGTCA ACTTCAACAA GAACAAAACT CCACTTGAGG GTGGCGAGAC CTACCACAAA 
GGATGTATCT TCTGCGACCC CATCTTGACC CCTCTCACGG AGGCCCAGCA TGCCAGGTTC 
CGTCGCAGGC GTGGCGGAGG AGGGGGTACC GTCGGGAGAG GGCGTGGTAG GGGGAGGGGC 
AGGGGGCGGG GCCGAGGAAG AGGTAGGGGA AAACCCAAGG ACAAGATGTC ACAGCTGGCC 
GCTTACTTTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011683116.1
CDS	45..2339
Translation	MPKFLAIEGKGCDYLVLWLDCDKEGENICFEVISCVQGVMTRPVGREQVIFRAKFSAITDKDIRQAMNTLVEPNENEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSTLISYGPCQTPTLGFCVNRHDKIQSFKPEPFWVIQIQVTHESGRKLNLDWDRVRVFDREVAQMFLNIIKTQKDARITSVSQKEKAKQRPQALNTVELMRVASSSLGMSPHHAMQIAERLYIQGYISYPRTETTSYPESFDLKGTLRQQMSAPWRSEVKVLLDGEFTKPRKGTDVGDHPPITPMRAANESEVGGEAWRLYEYIARHFIATVSPDCRYLLTTIRLMIGEEGFSCTGKTLISDGFTAVMPWQAIQDEENLPNVTKGDMCSIEEAKLSERQTSPPDYLTESELISLMEKHGVGTDASIPVHIENICQRNYVKVESGRRLVPTRLGIVLVHGYQKIDPDLSLPTMRSAVEEQLNLIAHGQADFNSVLIHTIDIFTRKFLYFVDHIQAMDDLFEVSFSPLAATGKPMSRCGKCHRFMKYISAKPMRLHCPTCEETYSLPQNGSIKLYKEIRCPLDDFELVLWSSGPKGKSYPVCPYCFSHPPFTEMKKGQGCNGCTHPTCAHSLTMLGVAQCIECDEGVMVLDPSSAPKWRLACNKCNIIMNIFEDAHKVTLSGHLCEKCDASILNVNFNKNKTPLEGGETYHKGCIFCDPILTPLTEAQHARFRRRRGGGGGTVGRGRGRGRGRGRGRGRGRGKPKDKMSQLAAYFV

Target ORF information:

RefSeq Version	XM_011684814.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus DNA topoisomerase 3-beta-1 (LOC591288), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011684814.1

ORF Insert Sequence:

ATGCCTAAAT TTCTCGCTAT AGAGGGCAAG GGCTGTGACT ACCTTGTGCT ATGGCTCGAC 
TGTGACAAGG AAGGTGAAAA CATCTGCTTT GAGGTTATCA GCTGCGTGCA GGGGGTCATG 
ACGAGACCAG TCGGAAGGGA ACAGGTAATC TTCCGTGCTA AATTCAGCGC TATCACGGAC 
AAGGACATCA GACAGGCCAT GAACACCCTG GTAGAGCCTA ACGAGAACGA AGCCCTCTCT 
GTCGACGCAA GGCAGGAACT GGATCTCAGG ATTGGATGTG CCTTCACCCG CTTCCAGACC 
AAGTACTTCC AGGGTAAATA TGGTGATCTC GACAGTACCT TAATCTCCTA CGGCCCTTGC 
CAGACTCCAA CCCTAGGCTT CTGTGTGAAC AGACACGACA AGATCCAGTC CTTCAAGCCT 
GAACCGTTCT GGGTCATTCA GATACAGGTA ACTCACGAGA GTGGAAGAAA GCTAAACCTT 
GACTGGGATC GAGTGAGAGT CTTTGACAGA GAAGTGGCTC AGATGTTTCT CAATATCATC 
AAAACACAGA AAGATGCAAG GATTACATCG GTGTCGCAGA AGGAGAAAGC CAAACAGCGC 
CCCCAGGCTC TCAATACGGT AGAGTTGATG CGTGTGGCGA GTTCATCGCT TGGTATGAGT 
CCCCACCATG CCATGCAGAT AGCCGAGAGG CTCTACATAC AGGGATACAT CAGCTACCCA 
AGAACAGAGA CAACAAGCTA TCCTGAGAGC TTTGACCTTA AGGGTACCTT GCGGCAACAG 
ATGTCGGCGC CCTGGAGGTC CGAAGTAAAG GTCCTCCTTG ACGGAGAATT CACCAAGCCC 
AGGAAAGGAA CCGACGTAGG AGATCATCCT CCTATCACGC CGATGAGAGC CGCTAACGAG 
AGTGAGGTGG GAGGAGAGGC GTGGAGGCTG TATGAGTATA TTGCCAGGCA CTTCATCGCT 
ACAGTGAGCC CCGACTGTCG CTACCTGCTC ACCACCATCC GTCTGATGAT CGGAGAGGAG 
GGCTTCAGCT GCACCGGTAA GACCCTGATC AGCGATGGCT TCACTGCAGT CATGCCCTGG 
CAGGCCATCC AAGATGAAGA GAACCTTCCC AACGTTACCA AGGGGGATAT GTGCTCCATT 
GAAGAGGCCA AGCTGAGTGA ACGCCAGACC AGCCCTCCCG ACTATCTGAC CGAAAGTGAA 
CTGATCTCCC TCATGGAGAA GCACGGTGTA GGTACAGACG CCAGCATACC CGTTCACATC 
GAGAACATCT GTCAGAGGAA CTATGTGAAG GTGGAGAGTG GGCGTCGTCT AGTACCCACA 
AGGCTTGGCA TTGTTCTGGT CCATGGATAT CAAAAGATTG ATCCAGACCT GTCTCTGCCC 
ACCATGAGAT CTGCGGTGGA GGAGCAGCTA AACCTGATCG CGCATGGCCA GGCCGACTTC 
AACTCTGTTC TGATACACAC CATAGACATC TTCACCAGGA AGTTCCTCTA CTTTGTAGAT 
CACATACAGG CCATGGATGA TCTCTTTGAG GTCTCCTTCT CGCCTCTGGC TGCCACCGGG 
AAACCCATGT CAAGATGCGG AAAGTGCCAT CGCTTCATGA AGTACATCTC GGCCAAGCCG 
ATGCGTCTTC ACTGCCCTAC CTGCGAAGAG ACGTACAGCC TCCCTCAGAA CGGTTCCATC 
AAGCTCTACA AAGAGATCAG GTGCCCCCTG GATGATTTTG AGCTGGTCCT GTGGTCTTCT 
GGGCCAAAGG GAAAGAGCTA CCCAGTGTGC CCCTACTGCT TCAGCCACCC CCCTTTCACG 
GAGATGAAGA AGGGACAGGG GTGCAACGGA TGCACCCATC CCACCTGTGC CCACTCCTTA 
ACCATGCTGG GCGTGGCTCA GTGTATCGAA TGCGATGAAG GGGTAATGGT GCTGGACCCC 
TCCTCGGCAC CCAAGTGGAG ACTTGCCTGC AACAAATGCA ACATCATCAT GAACATCTTC 
GAGGATGCTC ACAAGGTGAC CTTGTCGGGT CACCTGTGCG AAAAGTGTGA TGCTTCCATT 
CTAAATGTCA ACTTCAACAA GAACAAAACT CCACTTGAGG GTGGCGAGAC CTACCACAAA 
GGATGTATCT TCTGCGACCC CATCTTGACC CCTCTCACGG AGGCCCAGCA TGCCAGGTTC 
CGTCGCAGGC GTGGCGGAGG AGGGGGTACC GTCGGGAGAG GGCGTGGTAG GGGGAGGGGC 
AGGGGGCGGG GCCGAGGAAG AGGTAGGGGA AAACCCAAGG ACAAGATGTC ACAGCTGGCC 
GCTTACTTTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC591288 ( XM_030989368.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030845228.1 Strongylocentrotus purpuratus DNA topoisomerase 3-beta-1 (LOC591288), mRNA.

Price & Availability↑

CloneID	OSd55484
Clone ID Related Accession (Same CDS sequence)	XM_030989368.1
Accession Version	XM_030989368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	DNA topoisomerase 3-beta-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145603.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGG TTTTGATGGT GGCGGAGAAG CCACAGTTAG CCCAGTCTCT GGCTAAAATC 
CTGTCCCAAG GAAGTCTGCG ATCTCGCCGC GGAATGAATG GAGCGTGCAG TGTCCACGAG 
TACACGGGGA CGTTCCGGGG ACAGCAGGCT AAATTCAAGA TGACATCAGT GTGTGGTCAC 
GTCCATGGCC TGGACTTTCA TCCTCGTTTT AACAACTGGG ATAAAGTAGA TCCATCAGAG 
TTGTTTGCTG CCCCCACAGA GAGGAAGGAG GCCAATCCTA ATCTTCACAT GCCTAAATTT 
CTCGCTATAG AGGGCAAGGG CTGTGACTAC CTGGTGCTAT GGCTTGACTG TGATAAGGAA 
GGTGAAAACA TCTGCTTTGA GGTTATCAGC TGCGTACAGG GTGTCATGAC GAGACCAACC 
GGAAGGGAAC AGGTAATCTT CCGTGCTAAA TTCAGTGCCA TCACAGACAA GGACATCAGA 
CAGGCCATGA ACACCCTGGT AGAGCCTAAC GAGAACGAGG CCTTGTCTGT CGACGCAAGG 
CAGGAACTGG ATCTCAGGAT TGGATGCGCT TTTACTCGCT TCCAGACCAA GTACTTTCAG 
GGTAAATATG GTGATCTGGA CAGTACCCTG ATCTCCTACG GTCCTTGTCA GACTCCAACC 
CTTGGCTTCT GTGTGAACAG GCACGATAAG ATCCAGTCCT TCAAGCCTGA ACCCTTCTGG 
GTCATTCAGA TACAGGTAAC TCACGAGAGT GGAAGAAAGC TAAACCTTGA CTGGGATCGA 
GTGAGAGTCT TTGACAGAGA AGTGGCTCAG ATGTTTCTCA ATATCATCAA AACACAGAAA 
GATGCAAGGA TTACATCGGT GTCGCAGAAG GAGAAAGCCA AACAGCGCCC CCAGGCTCTC 
AATACGGTAG AGTTGATGCG TGTGGCGAGT TCATCGCTTG GTATGAGTCC CCACCATGCC 
ATGCAGATAG CCGAGAGGCT CTACATACAG GGATACATCA GCTACCCAAG AACAGAGACA 
ACAAGCTATC CTGAGAGCTT TGACCTTAAG GGTACCTTGC GGCAACAGAT GTCGGCGCCC 
TGGAGGTCCG AAGTAAAGGT CCTCCTTGAC GGAGAATTCA CCAAGCCCAG GAAAGGAACC 
GACGTAGGAG ATCATCCTCC TATCACGCCG ATGAGAGCCG CTAACGAGAG TGAGGTGGGA 
GGAGAGGCGT GGAGGCTGTA TGAGTATATT GCCAGGCACT TCATCGCTAC AGTGAGCCCC 
GACTGTCGCT ACCTGCTCAC CACCATCCGT CTGATGATCG GAGAGGAGGG CTTCAGCTGC 
ACCGGTAAGA CCCTGATCAG CGATGGCTTC ACTGCAGTCA TGCCCTGGCA GGCCATCCAA 
GATGAAGAGA ACCTTCCCAA CGTTACCAAG GGGGATATGT GCTCCATTGA AGAGGCCAAG 
CTGAGTGAAC GCCAGACCAG CCCTCCCGAC TATCTGACCG AAAGTGAACT GATCTCCCTC 
ATGGAGAAGC ACGGTGTAGG TACAGACGCC AGCATACCCG TTCACATCGA GAACATCTGT 
CAGAGGAACT ATGTGAAGGT GGAGAGTGGG CGTCGTCTAG TACCCACAAG GCTTGGCATT 
GTTCTGGTCC ATGGATATCA AAAGATTGAT CCAGACCTGT CTCTTCCCAC CATGCGATCT 
GCGGTGGAGG AGCAGCTAAA CCTGATCGCG CATGGCCAGG CCGACTTCAA CTCTGTTCTG 
ATACACACCA TAGACATCTT CACCAGGAAG TTCCTCTACT TTGTAGATCA CATACAAGCA 
ATGGACGATC TCTTTGAGGT CTCCTTCTCG CCTCTGGCTG CCACCGGGAA ACCCATGTCA 
AGATGCGGAA AGTGCCATCG CTTCATGAAG TACATCTCGG CCAAGCCGAT GCGTCTTCAC 
TGCCCTACCT GCGAAGAGAC GTACAGCCTC CCTCAGAACG GCTCCATCAA ACTCTACAAG 
GAGATCAGAT GCCCCCTGGA TGATTTCGAG CTGGTCCTGT GGTCTTCAGG GCCGAAGGGA 
AAGAGCTACC CGGTGTGCCC CTACTGCTTC AGCCACCCCC CTTTCACGGA GATGAAGAAG 
GGACAGGGAT GCAACGGATG CACCCATCCC ACATGTGCCC ACTCCTTAAC CATGCTGGGC 
GTGGCTCAGT GTATTGAGTG CGATGAGGGG GTAATGGTGT TGGACCCTTC CTCGGCACCC 
AAGTGGAGAC TCGCCTGCAA CAAATGCAAC ATCATCATGA ACATCTTCGA GGATGCTCAC 
AAGGTGACCT TGTCGGGTCA CCTGTGCGAA AAGTGTGATG CTTCCATTCT AAATGTCAAC 
TTCAACAAGA ACAAAACTCC ACTTGAGGGT GGCGAGACCT ACCACAAAGG ATGTATCTTC 
TGCGACCCCA TCTTGACCCC TCTCACGGAG GCCCAGCATG CCAGGTTCCG TCGCAGGCGT 
GGCGGAGGAG GGGGTACCGT CGGGAGAGGG CGTGGTAGGG GGAGGGGCAG GGGGCGGGGC 
CGAGGAAGAG GTAGGGGAAA ACCCAAGGAC AAGATGTCAC AGCTGGCCGC TTACTTTGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030845228.1
CDS	129..2711
Translation	MKEVLMVAEKPQLAQSLAKILSQGSLRSRRGMNGACSVHEYTGTFRGQQAKFKMTSVCGHVHGLDFHPRFNNWDKVDPSELFAAPTERKEANPNLHMPKFLAIEGKGCDYLVLWLDCDKEGENICFEVISCVQGVMTRPTGREQVIFRAKFSAITDKDIRQAMNTLVEPNENEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSTLISYGPCQTPTLGFCVNRHDKIQSFKPEPFWVIQIQVTHESGRKLNLDWDRVRVFDREVAQMFLNIIKTQKDARITSVSQKEKAKQRPQALNTVELMRVASSSLGMSPHHAMQIAERLYIQGYISYPRTETTSYPESFDLKGTLRQQMSAPWRSEVKVLLDGEFTKPRKGTDVGDHPPITPMRAANESEVGGEAWRLYEYIARHFIATVSPDCRYLLTTIRLMIGEEGFSCTGKTLISDGFTAVMPWQAIQDEENLPNVTKGDMCSIEEAKLSERQTSPPDYLTESELISLMEKHGVGTDASIPVHIENICQRNYVKVESGRRLVPTRLGIVLVHGYQKIDPDLSLPTMRSAVEEQLNLIAHGQADFNSVLIHTIDIFTRKFLYFVDHIQAMDDLFEVSFSPLAATGKPMSRCGKCHRFMKYISAKPMRLHCPTCEETYSLPQNGSIKLYKEIRCPLDDFELVLWSSGPKGKSYPVCPYCFSHPPFTEMKKGQGCNGCTHPTCAHSLTMLGVAQCIECDEGVMVLDPSSAPKWRLACNKCNIIMNIFEDAHKVTLSGHLCEKCDASILNVNFNKNKTPLEGGETYHKGCIFCDPILTPLTEAQHARFRRRRGGGGGTVGRGRGRGRGRGRGRGRGRGKPKDKMSQLAAYFV

Target ORF information:

RefSeq Version	XM_030989368.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus DNA topoisomerase 3-beta-1 (LOC591288), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030989368.1

ORF Insert Sequence:

ATGAAGGAGG TTTTGATGGT GGCGGAGAAG CCACAGTTAG CCCAGTCTCT GGCTAAAATC 
CTGTCCCAAG GAAGTCTGCG ATCTCGCCGC GGAATGAATG GAGCGTGCAG TGTCCACGAG 
TACACGGGGA CGTTCCGGGG ACAGCAGGCT AAATTCAAGA TGACATCAGT GTGTGGTCAC 
GTCCATGGCC TGGACTTTCA TCCTCGTTTT AACAACTGGG ATAAAGTAGA TCCATCAGAG 
TTGTTTGCTG CCCCCACAGA GAGGAAGGAG GCCAATCCTA ATCTTCACAT GCCTAAATTT 
CTCGCTATAG AGGGCAAGGG CTGTGACTAC CTGGTGCTAT GGCTTGACTG TGATAAGGAA 
GGTGAAAACA TCTGCTTTGA GGTTATCAGC TGCGTACAGG GTGTCATGAC GAGACCAACC 
GGAAGGGAAC AGGTAATCTT CCGTGCTAAA TTCAGTGCCA TCACAGACAA GGACATCAGA 
CAGGCCATGA ACACCCTGGT AGAGCCTAAC GAGAACGAGG CCTTGTCTGT CGACGCAAGG 
CAGGAACTGG ATCTCAGGAT TGGATGCGCT TTTACTCGCT TCCAGACCAA GTACTTTCAG 
GGTAAATATG GTGATCTGGA CAGTACCCTG ATCTCCTACG GTCCTTGTCA GACTCCAACC 
CTTGGCTTCT GTGTGAACAG GCACGATAAG ATCCAGTCCT TCAAGCCTGA ACCCTTCTGG 
GTCATTCAGA TACAGGTAAC TCACGAGAGT GGAAGAAAGC TAAACCTTGA CTGGGATCGA 
GTGAGAGTCT TTGACAGAGA AGTGGCTCAG ATGTTTCTCA ATATCATCAA AACACAGAAA 
GATGCAAGGA TTACATCGGT GTCGCAGAAG GAGAAAGCCA AACAGCGCCC CCAGGCTCTC 
AATACGGTAG AGTTGATGCG TGTGGCGAGT TCATCGCTTG GTATGAGTCC CCACCATGCC 
ATGCAGATAG CCGAGAGGCT CTACATACAG GGATACATCA GCTACCCAAG AACAGAGACA 
ACAAGCTATC CTGAGAGCTT TGACCTTAAG GGTACCTTGC GGCAACAGAT GTCGGCGCCC 
TGGAGGTCCG AAGTAAAGGT CCTCCTTGAC GGAGAATTCA CCAAGCCCAG GAAAGGAACC 
GACGTAGGAG ATCATCCTCC TATCACGCCG ATGAGAGCCG CTAACGAGAG TGAGGTGGGA 
GGAGAGGCGT GGAGGCTGTA TGAGTATATT GCCAGGCACT TCATCGCTAC AGTGAGCCCC 
GACTGTCGCT ACCTGCTCAC CACCATCCGT CTGATGATCG GAGAGGAGGG CTTCAGCTGC 
ACCGGTAAGA CCCTGATCAG CGATGGCTTC ACTGCAGTCA TGCCCTGGCA GGCCATCCAA 
GATGAAGAGA ACCTTCCCAA CGTTACCAAG GGGGATATGT GCTCCATTGA AGAGGCCAAG 
CTGAGTGAAC GCCAGACCAG CCCTCCCGAC TATCTGACCG AAAGTGAACT GATCTCCCTC 
ATGGAGAAGC ACGGTGTAGG TACAGACGCC AGCATACCCG TTCACATCGA GAACATCTGT 
CAGAGGAACT ATGTGAAGGT GGAGAGTGGG CGTCGTCTAG TACCCACAAG GCTTGGCATT 
GTTCTGGTCC ATGGATATCA AAAGATTGAT CCAGACCTGT CTCTTCCCAC CATGCGATCT 
GCGGTGGAGG AGCAGCTAAA CCTGATCGCG CATGGCCAGG CCGACTTCAA CTCTGTTCTG 
ATACACACCA TAGACATCTT CACCAGGAAG TTCCTCTACT TTGTAGATCA CATACAAGCA 
ATGGACGATC TCTTTGAGGT CTCCTTCTCG CCTCTGGCTG CCACCGGGAA ACCCATGTCA 
AGATGCGGAA AGTGCCATCG CTTCATGAAG TACATCTCGG CCAAGCCGAT GCGTCTTCAC 
TGCCCTACCT GCGAAGAGAC GTACAGCCTC CCTCAGAACG GCTCCATCAA ACTCTACAAG 
GAGATCAGAT GCCCCCTGGA TGATTTCGAG CTGGTCCTGT GGTCTTCAGG GCCGAAGGGA 
AAGAGCTACC CGGTGTGCCC CTACTGCTTC AGCCACCCCC CTTTCACGGA GATGAAGAAG 
GGACAGGGAT GCAACGGATG CACCCATCCC ACATGTGCCC ACTCCTTAAC CATGCTGGGC 
GTGGCTCAGT GTATTGAGTG CGATGAGGGG GTAATGGTGT TGGACCCTTC CTCGGCACCC 
AAGTGGAGAC TCGCCTGCAA CAAATGCAAC ATCATCATGA ACATCTTCGA GGATGCTCAC 
AAGGTGACCT TGTCGGGTCA CCTGTGCGAA AAGTGTGATG CTTCCATTCT AAATGTCAAC 
TTCAACAAGA ACAAAACTCC ACTTGAGGGT GGCGAGACCT ACCACAAAGG ATGTATCTTC 
TGCGACCCCA TCTTGACCCC TCTCACGGAG GCCCAGCATG CCAGGTTCCG TCGCAGGCGT 
GGCGGAGGAG GGGGTACCGT CGGGAGAGGG CGTGGTAGGG GGAGGGGCAG GGGGCGGGGC 
CGAGGAAGAG GTAGGGGAAA ACCCAAGGAC AAGATGTCAC AGCTGGCCGC TTACTTTGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC591288 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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