LOC590935 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC590935
Entrez Gene ID	590935
Full Name	phosphatidylinositol 4-kinase alpha
Synonyms	pi4ka
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_030991599.1	XP_030847459.1	phosphatidylinositol 4-kinase alpha isoform X2
XM_011665258.1	XP_011663560.1	phosphatidylinositol 4-kinase alpha
XM_030991598.1	XP_030847458.1	phosphatidylinositol 4-kinase alpha isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC590935 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC590935 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd26763	XM_011665258.1 Latest version!	Strongylocentrotus purpuratus phosphatidylinositol 4-kinase alpha (LOC590935), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSd57603	XM_030991598.1 Latest version!	Strongylocentrotus purpuratus phosphatidylinositol 4-kinase alpha (LOC590935), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSd57604	XM_030991599.1 Latest version!	Strongylocentrotus purpuratus phosphatidylinositol 4-kinase alpha (LOC590935), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC590935 ( XM_011665258.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011663560.1 Strongylocentrotus purpuratus phosphatidylinositol 4-kinase alpha (LOC590935), mRNA.

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CloneID	OSd26763
Clone ID Related Accession (Same CDS sequence)	XM_011665258.1
Accession Version	XM_011665258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	phosphatidylinositol 4-kinase alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011970416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGGGCCAA ACGGATTGTT CCCAGAAACG CTTCTACATT TAGCAAGATC ATTGGCCCAA 
ATCCCAGATC TGAAATGGCT AAAGGTGCAG CAACTGTTGC AGATGTGTCC CCAGATACCC 
GCTGTGGGAG GAGGGCTGTG CAGGATAGAC CAGCGTAGGC AGGATGCTAT GGTGGCGCTG 
GGGGTCTTCC TACTCGAATC AGACCTCAAG TTCAAGGACA AGTTGGTTCC ATATTTCATT 
AGTCTTGTGA GAAGCATACC CAATGCACAA TGGATTGAAG AAACGGGATA CAGGGTTATT 
GATGTATTAC CAATGAGTGA GCGCTTTGCA TTCACCATCA CAACTCTCCT TTCTGACATA 
GCGACCAAAG ACTCACACTA CAGAGATCAG ATTTTGTCAA CATTAGTTGA AGTTTTACAG 
GCACTATGCA GGATCTGCCA ACAGCCATCA GAACATCCTC AAGTTGCTAT ATGCAAGCAC 
GTCATGCCCG CATTCCTTGG GATGTCCCGT GCCATAGGGC GCAGCAGCCA GGACCCATCC 
TGCTCTCTCC TCAGTCGGAT GTACCCCTCA TCCAAGCACA CCGCCTCCCA GACCCCTCCC 
CCATCCTCCG GTTTGGACCA GACGTCCCCC ATGTACAGCT CCTTCCGACT CATCGTCTCC 
GACGTCATGG AGCAGAAACT CCGGCAGTCC GAAGGGAGCC GACAGGACGC ATTGCCTTTG 
GCAGACGACT CCAGGGAGGA GAGGGCGACG GAGGGACCTA GCCATCTGCT GACAGTGGTG 
GGGTCAAGTT TCGGTATCAG CACGGAGACA TGTGAGAGCG GGAGCGATGG AGCTCAAGTG 
GGTTCTCTGG ACCTCACCAC TCCACAGCTG CAGACCATCT TGAATGTGTG CCAAAAGATG 
ATCAGCGGAA CTCTGTTACA AAGTCTTGAT GGGATGGCCC AGCAGTACGC CCTTCAACCA 
GGATCAAAGA AGAACCTTCC GTACAAGTCA TATAGTGAAG TCATCAACGT GGTTCTGGTC 
TCTTTACTCA GACATCTCCT TCAACAGCAT AAAGGATTGC CAATGGGCTT CCAGACAGAG 
GTTCATGCGT TTGTCAGAGA ACTCTTTCTA TCGGGCCAAA CGGAGCTGCA GGATCTGAGT 
GTGGAGAGCG CCACCCGTGG TCCATGCAGG TTCAACTCTC GAGAGTTGGC ACTCAGGGCT 
AATGCAACAT GTGTGGACCT TCTTGTGTGG GCAGCTAATG ATGAAACAGG TGCTGATAGT 
CTTATTTCAA GGCTGACTGA GAAACTGCAT TCCAGTGCTG GCAGCAAGCA ACTCATAGCT 
GATATGCCTC TGCTTCTGTG CTCACTGCAG GGTTTGGGCA CTCTAGCAGA GAAGTTTCCG 
ACGGTCGCTC ACGCCACCAT CGGCTCCCTC CGCGACTTTC TGATGGGGCC GTCCCGCATC 
ATCGTCAAGC TCCACAAGCA TCATGAAGTC GAGAGCAGCA AGTCTAGTGC CGGACCCACC 
ATCAGGGTCA TGGACTCCAC CCTCCAGAGG GCTGGGGCTG GGAAGGATGG GCGCGGTGCT 
GGTGGTGGTG GTGGTGCCGG TAGTGGTGGT GGTTTTATGA GCAGCAAGAG TGTCGGGGAG 
GGGTCACATC CATATGAGAG ACTGAGGAAT GCGGCCATGT CCAACCTCTG CAGATCGCTG 
CGTTCTGGCC TCGTTGAAGA TCCCGATTGC ATTCAAGCCT TCCTAGCATC TGTCTCAAAT 
GCCCTCTACA TTGCTGAAGC CAGCGATCGA GATTCCACCC TCATCTCCTC CAATGCCATC 
TTGGCAATGG GCCACGTGGC AGTTCTTTTG AGCGAAACAA CTCGTACCAT CGAGAGCGTC 
CAGCAGATTT TCCAGCAACG GTTCTGCACG CCCCCTTCCT CCCTCGATGT GCTGATTGTG 
GATATGCTAG GATGCCTGGT GCTGGCTGGA AATGCTGCTG TAACGCAGGA GGTGATGAAT 
ATGTTTATTC AGATCAGTGT CGAAGCCGGA TCATCTGATT ATGCACCTAA CAGCTCTCAG 
GCTTCACAGA GATTCAGGCA TTGCTCCATG GCTATCATCA ATGCCCTTGC AAACATAGCA 
GCATTTATAG ACGGAGAGAA CGAACAGCAG GAGCTCTTAG TCCGGCTACT TGAATTATTT 
GTCCAGCTGG GACTAGAGGG CAAGCGGGCC AGTGAGAGGG CTCCTACAGC TATCAAGGCC 
TCCAGCAGTG CTGGAAGCTT GGGCATCCTT ATTCCTGTCC TTGCAACTTT GATCAAGAGA 
CTGCCACCCA TCCAGGACCC AAAGCCTCGC CTTCACAAGT TCTTCAGGGA TTTCTGGCTT 
TACTGTGTGG TCATGGGGTT TGCCGTGGAG GACTCTGGTA TCAAGATTTG GAATATTGTA 
CCTCAAACTA CACGCCGTCT CTGGCCCAAA GAGTGGTACG AGGGAGTCTG TCACATCGCC 
GCAAAGTCTC CCCTCCTTAC GTCCAGTCCT GGTGAACATC TGAGATCCGA GCTACAGTAC 
AACTCCGCTC TCAGGAACGA TAGCATGACA TATAACGACC TGAACGAGCT CCGTCAGAAC 
ATCCTGGGCC TGATGGAGCA TTCCCCAGAG GTCACCGTCA TCGTCAACAA GCTCAACTTT 
GCCCAGTGTA CCTACCTGCT CTCTGTGCAT AGGCTGGAGA CCCTGCGGGT GAGTAACGCC 
GTCCCCACCA GCTTCGCAGC AGTCTTCAGC TACCTGGAAG ACAAGGCGAT CATCAAGGAC 
AAGGCTGGCA TGTGGCAGTG TCTCCTGGCA TCCTTCGACA AGGTCTTCGA CATGTTCTTG 
GATATCAAGG CCAAAATGCC CCGTACTGAA GAGAGGGAGA AGGAGCTGGT CCATCATGCT 
CAGTTCCTGC TTGTCAAGTT CAATCATGTC CTCAAACAGA TCAGGAAGGT TGCTGATAAA 
TACCTTTCAA GACTTGTAGA CAGGTTTCCT CAGTTACTGT GGAATGGTGA TGTCCTTTGG 
AGCATGTTGG ATCTCCTGCA GCTCTTGTCC AAGGCTGCTG CAGAGGAGAG TCTTCAGGAG 
GTCAGAGAGT TCCCTGTTTT CAAGGGCATG TTCATGCTGA CAGTCATGGA CACCAAGGAA 
TCAAGAGAGG GCATTGTCCG TGACTTTGCA GCCCGTTGCG GAGGTATTTT CCAGGAGGCC 
GTGAAGTGGG CGGCCTATGC GACGCGCTCT CACATCCAGC AGTACATCAT CCATCTGGAG 
AACTCCTCGG ACAGTCTCTC ACAGCGTAGC AACCTGGAGC TCGGCACGGA GAGTGCCTTT 
CAGTGTGCAG AATTCACCAA AGGGACTCAC CGATTCAACA AAGGACTCAC CGCATCTGGT 
ACCGACAGTT TGACCAGTGA GTCAGCCAAC TTTGTATCAG TGATGAGCCT AAGGAGCAGG 
TTCGCTGGAG AGGTATCAGG CATGAAAGCA ATCTACACCG ACGCAGGGCC CGGGGGTTTG 
AGTCTATCTC ATGTACTGAA TAAACAGCTT GACAAGGCGT GTGATGCGAA GAAGATAGAT 
GCTTTCACTA ATTCTATGTT CAGAGTCTGT GCCCTCCTCA TCTCAAGTGA AGGTGTGGAT 
AGATACCTGT TGTATAGCCT GTGCAGGGCG CCTGTCAGGC TCTTCACAAC ACACGCTATG 
GAGAAGGCTG TTGCATGCTG GGAATGGCTG CTGGCTGCCA GATCGGACAT GGAACTGCAG 
TTAATGCGTG AGATGGCCGG AGCATGGCAG ATGAGCATGG ACAACAGACT TGGTCTGTTC 
TCTATTGATG TTGAAGATCC CAGTCCTCTG GCCGCCATCA GTGGCGATGA TGTCCCTGAC 
CCCAAACACC CAAACGCTAC ACCACATCAC ATCTGGACCC AGTTCTTACA CCAGCGCCTG 
GAGAGCATCA AGTACAGCAG CATCAACGTG GTGGAGATCT TCATCTCCAT CCTGCATCGC 
TCCCTACCCA TGCAGGTCGG TCAGACAAAG GGCGCCCTCT CCAGGCACAT CGCTGCCATG 
GGCCCCCGTT TCCGCCTGCT CTGTATGGGA CTGTCCCTCC TGCAGGGGGA TATCCTGCCC 
AGCAGTACGG GCAAGAACGT CTTGAGAGAA AGGATCTACT CGGCTGCGTT CGACTTCTTC 
AGTGTTGCTC CAGTGTGCCC AACCCAGTCT GTGAGTGAGC TGAGGGAAGA CATCAGTATT 
CTCATTAAGT TCTTCCATGC CATGCTGGCA GAGAAGAAAT ACCTCAGAGG CACCACAGCT 
GTGGTTCAAG AGTCCGAGGA TGTACACACG CTAGGCATCA TCCCAGCCGA CCTGCGAGCC 
AGTAACGAGT ACCTCAACGC CAGGCCAACA GCAGGGCGCC CTCAGGGTTG GATGAACACC 
ATGCCGCTGT CCAGTAACAT GTCCATCGTG TCCAAGAGGT CATCGGTCAC CAGGAAGAGT 
GGAGAGGTCG GAGCAAAGTA TGCCAAGGAG TACATGAAGA GGAGGAATCT AATCCTCTCT 
CTGCTGGTGA ATGAGATAGA GAGACTGTGT TGCTGGTGTA ATCCCTTAGA CAACTCAGAA 
ATCAAGCTAC CAGGAGAGGA GCTGGTTGCT AACTGGAGGG TCCAGACCCC TACCAATGAA 
CGCACCTGGA GGGACTTTGC TCACCTCGCC TGGGAGGTGT CCCCTCATCT GGCTTGTCAG 
CTTCCCGTCA GGTTCAAGTC TTCTGAGAGC CTTGTCAAGG AGGTGACGCG TATGGTGCGA 
CTCAACCCCA TGGCTGTCTC TGACCTCCCA AGTGCCATTC ACTTCCTGGT CACAGAACAC 
AGTGTGGATG CAGATGTCGC AGAGTTGAGT CACATCCTAT GCTGGGCGCC CGTTCCACCT 
GTCACCGCCC TGTCATACTT CTCCCAGCAG TACCCCCCGC ACCCCCTGAC TGCCCAGTAT 
GCCACCAGAG TCCTGCAGGA CTACCCACCA GAGGCACTGC TCTTCTATGT CCCTCAGCTA 
GTACAGGCTG TCAGATACGA TACGTTTGGC TTTGTGACGG AGCTGATCCT GGTCCTGGCG 
AAAAAGTCCC AACTCCTGGC CCATCAGCTC ATCTGGAACA TGAAGACCAA CATCTTCAAA 
GACGAGGAAG CCAAGGAAAG AGACGGTGAG TTTGCTGATG CCCTGGAGGC CCTGATCGAA 
GGCATCATAG CCAGCTTGTC GGGACCAGCG AAAGACTTCT ATGAGAGGGA GTTTGACTTT 
TTTGGCAAAA TAACAGCCAT CTCCGGCGAG ATCAGACCAT ATCCCAAGGG TGATGAAAGG 
AAAGCAGCCT GTCTGAAAGC CCTAGCAACC ATTCAACTCC AACCTGGATG CTATCTCCCT 
TGCAACCCTG AAGCCGTCGT CATGGAAATC GACTACAAGT CTGGAACTCT CATGCAGAGT 
GCTGCCAAGG CCCCGTTCCT TGCCAGGTTC AAGGTGCAGA AGTGCGGAGT TCATGGGCTG 
GAGAGCCTTG GTATGAAGGG CCTCGATTCA GACGCTGTTT CTCGTTCCCT CAAAGCACAA 
ATATGGGAGG CTTGCATCTT TAAGGTTGGA GATGACGTGA GACAGGACAT GCTTGCGCTG 
CAGGTGATTG GTCTATTCAA GAACATCTTC CATCAGGTGG GACTCAACCT CTACCTGGTC 
CCGTACAAAG TGGTAGCAAC GTCACCAGGG TGTGGAGTGA TCCAGTGTGT CCCAGACTGC 
AAGTCTCGTG ACCAGCTTGG CCGACAGACA GACATCGGCA TGTATGAATA CTTCCTAACC 
AAGTATGGAG ATGAGACTAC ACCCGCATAC CAGAAGGCTA GACGTCATTT CATCCAAAGC 
ATGGCAGCCT ACAGTGTGGT AGGGCACCTC CTCCAAATCA AAGACAGACA TAATGGAAAT 
ATCATGCTGG ATACCAGAGG CAACATCATA CACATTGATT TTGGTTTCAT GTTTGAGAGT 
TCTCCTGGAG GCAACCTGGG CTGGGAACCA GACATCAAGC TCACTGATGA AATGCTCATG 
ATCATGGGAG GCAATATAAA CGCGGCTCCA TTCAAGAAGT TTGAAGAACT GTGTGTACAG 
GCCTACCTAG CAGTCAGACC ATACCAAGAA GCCATTGTGT CCCTTGTAGC CCTGATGCTT 
GATACAGGAC TACCTTGCTT CAAAGGAAAA ACACTCAAAC TACTAAGGAC TCGCTTCAGC 
CCGAACCAGA CAGAAAGGGA AGCTGCAATC TACATGGTTG GCATCATAGA AAAGTGTTGC 
CAGAATGTCA GGACCAGAAC CTATGACATG ATCCAGTACA TCCAAAATGA GATTCCCTAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011663560.1
CDS	15..6317
Translation	MGPNGLFPETLLHLARSLAQIPDLKWLKVQQLLQMCPQIPAVGGGLCRIDQRRQDAMVALGVFLLESDLKFKDKLVPYFISLVRSIPNAQWIEETGYRVIDVLPMSERFAFTITTLLSDIATKDSHYRDQILSTLVEVLQALCRICQQPSEHPQVAICKHVMPAFLGMSRAIGRSSQDPSCSLLSRMYPSSKHTASQTPPPSSGLDQTSPMYSSFRLIVSDVMEQKLRQSEGSRQDALPLADDSREERATEGPSHLLTVVGSSFGISTETCESGSDGAQVGSLDLTTPQLQTILNVCQKMISGTLLQSLDGMAQQYALQPGSKKNLPYKSYSEVINVVLVSLLRHLLQQHKGLPMGFQTEVHAFVRELFLSGQTELQDLSVESATRGPCRFNSRELALRANATCVDLLVWAANDETGADSLISRLTEKLHSSAGSKQLIADMPLLLCSLQGLGTLAEKFPTVAHATIGSLRDFLMGPSRIIVKLHKHHEVESSKSSAGPTIRVMDSTLQRAGAGKDGRGAGGGGGAGSGGGFMSSKSVGEGSHPYERLRNAAMSNLCRSLRSGLVEDPDCIQAFLASVSNALYIAEASDRDSTLISSNAILAMGHVAVLLSETTRTIESVQQIFQQRFCTPPSSLDVLIVDMLGCLVLAGNAAVTQEVMNMFIQISVEAGSSDYAPNSSQASQRFRHCSMAIINALANIAAFIDGENEQQELLVRLLELFVQLGLEGKRASERAPTAIKASSSAGSLGILIPVLATLIKRLPPIQDPKPRLHKFFRDFWLYCVVMGFAVEDSGIKIWNIVPQTTRRLWPKEWYEGVCHIAAKSPLLTSSPGEHLRSELQYNSALRNDSMTYNDLNELRQNILGLMEHSPEVTVIVNKLNFAQCTYLLSVHRLETLRVSNAVPTSFAAVFSYLEDKAIIKDKAGMWQCLLASFDKVFDMFLDIKAKMPRTEEREKELVHHAQFLLVKFNHVLKQIRKVADKYLSRLVDRFPQLLWNGDVLWSMLDLLQLLSKAAAEESLQEVREFPVFKGMFMLTVMDTKESREGIVRDFAARCGGIFQEAVKWAAYATRSHIQQYIIHLENSSDSLSQRSNLELGTESAFQCAEFTKGTHRFNKGLTASGTDSLTSESANFVSVMSLRSRFAGEVSGMKAIYTDAGPGGLSLSHVLNKQLDKACDAKKIDAFTNSMFRVCALLISSEGVDRYLLYSLCRAPVRLFTTHAMEKAVACWEWLLAARSDMELQLMREMAGAWQMSMDNRLGLFSIDVEDPSPLAAISGDDVPDPKHPNATPHHIWTQFLHQRLESIKYSSINVVEIFISILHRSLPMQVGQTKGALSRHIAAMGPRFRLLCMGLSLLQGDILPSSTGKNVLRERIYSAAFDFFSVAPVCPTQSVSELREDISILIKFFHAMLAEKKYLRGTTAVVQESEDVHTLGIIPADLRASNEYLNARPTAGRPQGWMNTMPLSSNMSIVSKRSSVTRKSGEVGAKYAKEYMKRRNLILSLLVNEIERLCCWCNPLDNSEIKLPGEELVANWRVQTPTNERTWRDFAHLAWEVSPHLACQLPVRFKSSESLVKEVTRMVRLNPMAVSDLPSAIHFLVTEHSVDADVAELSHILCWAPVPPVTALSYFSQQYPPHPLTAQYATRVLQDYPPEALLFYVPQLVQAVRYDTFGFVTELILVLAKKSQLLAHQLIWNMKTNIFKDEEAKERDGEFADALEALIEGIIASLSGPAKDFYEREFDFFGKITAISGEIRPYPKGDERKAACLKALATIQLQPGCYLPCNPEAVVMEIDYKSGTLMQSAAKAPFLARFKVQKCGVHGLESLGMKGLDSDAVSRSLKAQIWEACIFKVGDDVRQDMLALQVIGLFKNIFHQVGLNLYLVPYKVVATSPGCGVIQCVPDCKSRDQLGRQTDIGMYEYFLTKYGDETTPAYQKARRHFIQSMAAYSVVGHLLQIKDRHNGNIMLDTRGNIIHIDFGFMFESSPGGNLGWEPDIKLTDEMLMIMGGNINAAPFKKFEELCVQAYLAVRPYQEAIVSLVALMLDTGLPCFKGKTLKLLRTRFSPNQTEREAAIYMVGIIEKCCQNVRTRTYDMIQYIQNEIPY

Target ORF information:

RefSeq Version	XM_011665258.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus phosphatidylinositol 4-kinase alpha (LOC590935), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011665258.1

ORF Insert Sequence:

ATGGGGCCAA ACGGATTGTT CCCAGAAACG CTTCTACATT TAGCAAGATC ATTGGCCCAA 
ATCCCAGATC TGAAATGGCT AAAGGTGCAG CAACTGTTGC AGATGTGTCC CCAGATACCC 
GCTGTGGGAG GAGGGCTGTG CAGGATAGAC CAGCGTAGGC AGGATGCTAT GGTGGCGCTG 
GGGGTCTTCC TACTCGAATC AGACCTCAAG TTCAAGGACA AGTTGGTTCC ATATTTCATT 
AGTCTTGTGA GAAGCATACC CAATGCACAA TGGATTGAAG AAACGGGATA CAGGGTTATT 
GATGTATTAC CAATGAGTGA GCGCTTTGCA TTCACCATCA CAACTCTCCT TTCTGACATA 
GCGACCAAAG ACTCACACTA CAGAGATCAG ATTTTGTCAA CATTAGTTGA AGTTTTACAG 
GCACTATGCA GGATCTGCCA ACAGCCATCA GAACATCCTC AAGTTGCTAT ATGCAAGCAC 
GTCATGCCCG CATTCCTTGG GATGTCCCGT GCCATAGGGC GCAGCAGCCA GGACCCATCC 
TGCTCTCTCC TCAGTCGGAT GTACCCCTCA TCCAAGCACA CCGCCTCCCA GACCCCTCCC 
CCATCCTCCG GTTTGGACCA GACGTCCCCC ATGTACAGCT CCTTCCGACT CATCGTCTCC 
GACGTCATGG AGCAGAAACT CCGGCAGTCC GAAGGGAGCC GACAGGACGC ATTGCCTTTG 
GCAGACGACT CCAGGGAGGA GAGGGCGACG GAGGGACCTA GCCATCTGCT GACAGTGGTG 
GGGTCAAGTT TCGGTATCAG CACGGAGACA TGTGAGAGCG GGAGCGATGG AGCTCAAGTG 
GGTTCTCTGG ACCTCACCAC TCCACAGCTG CAGACCATCT TGAATGTGTG CCAAAAGATG 
ATCAGCGGAA CTCTGTTACA AAGTCTTGAT GGGATGGCCC AGCAGTACGC CCTTCAACCA 
GGATCAAAGA AGAACCTTCC GTACAAGTCA TATAGTGAAG TCATCAACGT GGTTCTGGTC 
TCTTTACTCA GACATCTCCT TCAACAGCAT AAAGGATTGC CAATGGGCTT CCAGACAGAG 
GTTCATGCGT TTGTCAGAGA ACTCTTTCTA TCGGGCCAAA CGGAGCTGCA GGATCTGAGT 
GTGGAGAGCG CCACCCGTGG TCCATGCAGG TTCAACTCTC GAGAGTTGGC ACTCAGGGCT 
AATGCAACAT GTGTGGACCT TCTTGTGTGG GCAGCTAATG ATGAAACAGG TGCTGATAGT 
CTTATTTCAA GGCTGACTGA GAAACTGCAT TCCAGTGCTG GCAGCAAGCA ACTCATAGCT 
GATATGCCTC TGCTTCTGTG CTCACTGCAG GGTTTGGGCA CTCTAGCAGA GAAGTTTCCG 
ACGGTCGCTC ACGCCACCAT CGGCTCCCTC CGCGACTTTC TGATGGGGCC GTCCCGCATC 
ATCGTCAAGC TCCACAAGCA TCATGAAGTC GAGAGCAGCA AGTCTAGTGC CGGACCCACC 
ATCAGGGTCA TGGACTCCAC CCTCCAGAGG GCTGGGGCTG GGAAGGATGG GCGCGGTGCT 
GGTGGTGGTG GTGGTGCCGG TAGTGGTGGT GGTTTTATGA GCAGCAAGAG TGTCGGGGAG 
GGGTCACATC CATATGAGAG ACTGAGGAAT GCGGCCATGT CCAACCTCTG CAGATCGCTG 
CGTTCTGGCC TCGTTGAAGA TCCCGATTGC ATTCAAGCCT TCCTAGCATC TGTCTCAAAT 
GCCCTCTACA TTGCTGAAGC CAGCGATCGA GATTCCACCC TCATCTCCTC CAATGCCATC 
TTGGCAATGG GCCACGTGGC AGTTCTTTTG AGCGAAACAA CTCGTACCAT CGAGAGCGTC 
CAGCAGATTT TCCAGCAACG GTTCTGCACG CCCCCTTCCT CCCTCGATGT GCTGATTGTG 
GATATGCTAG GATGCCTGGT GCTGGCTGGA AATGCTGCTG TAACGCAGGA GGTGATGAAT 
ATGTTTATTC AGATCAGTGT CGAAGCCGGA TCATCTGATT ATGCACCTAA CAGCTCTCAG 
GCTTCACAGA GATTCAGGCA TTGCTCCATG GCTATCATCA ATGCCCTTGC AAACATAGCA 
GCATTTATAG ACGGAGAGAA CGAACAGCAG GAGCTCTTAG TCCGGCTACT TGAATTATTT 
GTCCAGCTGG GACTAGAGGG CAAGCGGGCC AGTGAGAGGG CTCCTACAGC TATCAAGGCC 
TCCAGCAGTG CTGGAAGCTT GGGCATCCTT ATTCCTGTCC TTGCAACTTT GATCAAGAGA 
CTGCCACCCA TCCAGGACCC AAAGCCTCGC CTTCACAAGT TCTTCAGGGA TTTCTGGCTT 
TACTGTGTGG TCATGGGGTT TGCCGTGGAG GACTCTGGTA TCAAGATTTG GAATATTGTA 
CCTCAAACTA CACGCCGTCT CTGGCCCAAA GAGTGGTACG AGGGAGTCTG TCACATCGCC 
GCAAAGTCTC CCCTCCTTAC GTCCAGTCCT GGTGAACATC TGAGATCCGA GCTACAGTAC 
AACTCCGCTC TCAGGAACGA TAGCATGACA TATAACGACC TGAACGAGCT CCGTCAGAAC 
ATCCTGGGCC TGATGGAGCA TTCCCCAGAG GTCACCGTCA TCGTCAACAA GCTCAACTTT 
GCCCAGTGTA CCTACCTGCT CTCTGTGCAT AGGCTGGAGA CCCTGCGGGT GAGTAACGCC 
GTCCCCACCA GCTTCGCAGC AGTCTTCAGC TACCTGGAAG ACAAGGCGAT CATCAAGGAC 
AAGGCTGGCA TGTGGCAGTG TCTCCTGGCA TCCTTCGACA AGGTCTTCGA CATGTTCTTG 
GATATCAAGG CCAAAATGCC CCGTACTGAA GAGAGGGAGA AGGAGCTGGT CCATCATGCT 
CAGTTCCTGC TTGTCAAGTT CAATCATGTC CTCAAACAGA TCAGGAAGGT TGCTGATAAA 
TACCTTTCAA GACTTGTAGA CAGGTTTCCT CAGTTACTGT GGAATGGTGA TGTCCTTTGG 
AGCATGTTGG ATCTCCTGCA GCTCTTGTCC AAGGCTGCTG CAGAGGAGAG TCTTCAGGAG 
GTCAGAGAGT TCCCTGTTTT CAAGGGCATG TTCATGCTGA CAGTCATGGA CACCAAGGAA 
TCAAGAGAGG GCATTGTCCG TGACTTTGCA GCCCGTTGCG GAGGTATTTT CCAGGAGGCC 
GTGAAGTGGG CGGCCTATGC GACGCGCTCT CACATCCAGC AGTACATCAT CCATCTGGAG 
AACTCCTCGG ACAGTCTCTC ACAGCGTAGC AACCTGGAGC TCGGCACGGA GAGTGCCTTT 
CAGTGTGCAG AATTCACCAA AGGGACTCAC CGATTCAACA AAGGACTCAC CGCATCTGGT 
ACCGACAGTT TGACCAGTGA GTCAGCCAAC TTTGTATCAG TGATGAGCCT AAGGAGCAGG 
TTCGCTGGAG AGGTATCAGG CATGAAAGCA ATCTACACCG ACGCAGGGCC CGGGGGTTTG 
AGTCTATCTC ATGTACTGAA TAAACAGCTT GACAAGGCGT GTGATGCGAA GAAGATAGAT 
GCTTTCACTA ATTCTATGTT CAGAGTCTGT GCCCTCCTCA TCTCAAGTGA AGGTGTGGAT 
AGATACCTGT TGTATAGCCT GTGCAGGGCG CCTGTCAGGC TCTTCACAAC ACACGCTATG 
GAGAAGGCTG TTGCATGCTG GGAATGGCTG CTGGCTGCCA GATCGGACAT GGAACTGCAG 
TTAATGCGTG AGATGGCCGG AGCATGGCAG ATGAGCATGG ACAACAGACT TGGTCTGTTC 
TCTATTGATG TTGAAGATCC CAGTCCTCTG GCCGCCATCA GTGGCGATGA TGTCCCTGAC 
CCCAAACACC CAAACGCTAC ACCACATCAC ATCTGGACCC AGTTCTTACA CCAGCGCCTG 
GAGAGCATCA AGTACAGCAG CATCAACGTG GTGGAGATCT TCATCTCCAT CCTGCATCGC 
TCCCTACCCA TGCAGGTCGG TCAGACAAAG GGCGCCCTCT CCAGGCACAT CGCTGCCATG 
GGCCCCCGTT TCCGCCTGCT CTGTATGGGA CTGTCCCTCC TGCAGGGGGA TATCCTGCCC 
AGCAGTACGG GCAAGAACGT CTTGAGAGAA AGGATCTACT CGGCTGCGTT CGACTTCTTC 
AGTGTTGCTC CAGTGTGCCC AACCCAGTCT GTGAGTGAGC TGAGGGAAGA CATCAGTATT 
CTCATTAAGT TCTTCCATGC CATGCTGGCA GAGAAGAAAT ACCTCAGAGG CACCACAGCT 
GTGGTTCAAG AGTCCGAGGA TGTACACACG CTAGGCATCA TCCCAGCCGA CCTGCGAGCC 
AGTAACGAGT ACCTCAACGC CAGGCCAACA GCAGGGCGCC CTCAGGGTTG GATGAACACC 
ATGCCGCTGT CCAGTAACAT GTCCATCGTG TCCAAGAGGT CATCGGTCAC CAGGAAGAGT 
GGAGAGGTCG GAGCAAAGTA TGCCAAGGAG TACATGAAGA GGAGGAATCT AATCCTCTCT 
CTGCTGGTGA ATGAGATAGA GAGACTGTGT TGCTGGTGTA ATCCCTTAGA CAACTCAGAA 
ATCAAGCTAC CAGGAGAGGA GCTGGTTGCT AACTGGAGGG TCCAGACCCC TACCAATGAA 
CGCACCTGGA GGGACTTTGC TCACCTCGCC TGGGAGGTGT CCCCTCATCT GGCTTGTCAG 
CTTCCCGTCA GGTTCAAGTC TTCTGAGAGC CTTGTCAAGG AGGTGACGCG TATGGTGCGA 
CTCAACCCCA TGGCTGTCTC TGACCTCCCA AGTGCCATTC ACTTCCTGGT CACAGAACAC 
AGTGTGGATG CAGATGTCGC AGAGTTGAGT CACATCCTAT GCTGGGCGCC CGTTCCACCT 
GTCACCGCCC TGTCATACTT CTCCCAGCAG TACCCCCCGC ACCCCCTGAC TGCCCAGTAT 
GCCACCAGAG TCCTGCAGGA CTACCCACCA GAGGCACTGC TCTTCTATGT CCCTCAGCTA 
GTACAGGCTG TCAGATACGA TACGTTTGGC TTTGTGACGG AGCTGATCCT GGTCCTGGCG 
AAAAAGTCCC AACTCCTGGC CCATCAGCTC ATCTGGAACA TGAAGACCAA CATCTTCAAA 
GACGAGGAAG CCAAGGAAAG AGACGGTGAG TTTGCTGATG CCCTGGAGGC CCTGATCGAA 
GGCATCATAG CCAGCTTGTC GGGACCAGCG AAAGACTTCT ATGAGAGGGA GTTTGACTTT 
TTTGGCAAAA TAACAGCCAT CTCCGGCGAG ATCAGACCAT ATCCCAAGGG TGATGAAAGG 
AAAGCAGCCT GTCTGAAAGC CCTAGCAACC ATTCAACTCC AACCTGGATG CTATCTCCCT 
TGCAACCCTG AAGCCGTCGT CATGGAAATC GACTACAAGT CTGGAACTCT CATGCAGAGT 
GCTGCCAAGG CCCCGTTCCT TGCCAGGTTC AAGGTGCAGA AGTGCGGAGT TCATGGGCTG 
GAGAGCCTTG GTATGAAGGG CCTCGATTCA GACGCTGTTT CTCGTTCCCT CAAAGCACAA 
ATATGGGAGG CTTGCATCTT TAAGGTTGGA GATGACGTGA GACAGGACAT GCTTGCGCTG 
CAGGTGATTG GTCTATTCAA GAACATCTTC CATCAGGTGG GACTCAACCT CTACCTGGTC 
CCGTACAAAG TGGTAGCAAC GTCACCAGGG TGTGGAGTGA TCCAGTGTGT CCCAGACTGC 
AAGTCTCGTG ACCAGCTTGG CCGACAGACA GACATCGGCA TGTATGAATA CTTCCTAACC 
AAGTATGGAG ATGAGACTAC ACCCGCATAC CAGAAGGCTA GACGTCATTT CATCCAAAGC 
ATGGCAGCCT ACAGTGTGGT AGGGCACCTC CTCCAAATCA AAGACAGACA TAATGGAAAT 
ATCATGCTGG ATACCAGAGG CAACATCATA CACATTGATT TTGGTTTCAT GTTTGAGAGT 
TCTCCTGGAG GCAACCTGGG CTGGGAACCA GACATCAAGC TCACTGATGA AATGCTCATG 
ATCATGGGAG GCAATATAAA CGCGGCTCCA TTCAAGAAGT TTGAAGAACT GTGTGTACAG 
GCCTACCTAG CAGTCAGACC ATACCAAGAA GCCATTGTGT CCCTTGTAGC CCTGATGCTT 
GATACAGGAC TACCTTGCTT CAAAGGAAAA ACACTCAAAC TACTAAGGAC TCGCTTCAGC 
CCGAACCAGA CAGAAAGGGA AGCTGCAATC TACATGGTTG GCATCATAGA AAAGTGTTGC 
CAGAATGTCA GGACCAGAAC CTATGACATG ATCCAGTACA TCCAAAATGA GATTCCCTAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC590935 ( XM_030991598.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030847458.1 Strongylocentrotus purpuratus phosphatidylinositol 4-kinase alpha (LOC590935), transcript variant X1, mRNA.

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CloneID	OSd57603
Clone ID Related Accession (Same CDS sequence)	XM_030991598.1
Accession Version	XM_030991598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	phosphatidylinositol 4-kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145605.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCAA ACGGATTGTT CCCAGAAACG CTTCTACATT TAGCAAGATC ATTGGCCCAA 
ATCCCAGATC TGAAATGGCT AAAGGTGCAG CAACTGTTGC AGATGTGTCC CCAGATACCC 
GCTGTGGGAG GAGGGCTGTG CAGGATAGAC CAGCGTAGGC AGGATGCTAT GGTGGCGCTG 
GGGGTCTTCC TACTCGAATC AGACCTCAAG TTCAAGGACA AGTTGGTTCC ATATTTCATT 
AGTCTTGTGA GAAGCATACC CAATGCACAA TGGATTGAAG AAACGGGATA CAGGGTTATT 
GATGTATTAC CAATGAGTGA GCGCTTTGCA TTCACCATCA CAACTCTCCT TTCTGACATA 
GCGACCAAAG ACTCACACTA CAGAGATCAG ATTTTGTCAA CATTAGTTGA AGTTTTACAG 
GCACTATGCA GGATCTGCCA ACAGCCATCA GAACATCCTC AAGTTGCTAT ATGCAAGCAC 
GTCATGCCCG CATTCCTTGG GATGTCCCGT GCCATAGGGC GCAGCAGCCA GGACCCATCC 
TGCTCTCTCC TCAGTCGGAT GTACCCCTCA TCCAAGCACA CCGCCTCCCA GACCCCTCCC 
CCATCCTCCG GTTTGGACCA GACGTCCCCC ATGTACAGCT CCTTCCGACT CATCGTCTCC 
GACGTCATGG AGCAGAAACT CCGGCAGTCC GAAGGGAGCC GACAGGACGC ATTGCCTTTG 
GCAGACGACT CCAGGGAGGA GAGGGCGACG GAGGGACCTA GCCATCTGCT GACAGTGGTG 
GGGTCAAGTT TCGGTATCAG CACGGAGACA TGTGAGAGCG GGAGCGATGG AGCTCAAGTG 
GGTTCTCTGG ACCTCACCAC TCCACAGCTG CAGACCATCT TGAATGTGTG CCAAAAGATG 
ATCAGCGGAA CTCTGTTACA AAGTCTTGAT GGGATGGCCC AGCAGTACGC CCTTCAACCA 
GGATCAAAGA AGAACCTTCC GTACAAGTCA TATAGTGAAG TCATCAACGT GGTTCTGGTC 
TCTTTACTCA GACATCTCCT TCAACAGCAT AAAGGATTGC CAATGGGCTT CCAGACAGAG 
GTTCATGCGT TTGTCAGAGA ACTCTTTCTA TCGGGCCAAA CGGAGCTGCA GGATCTGAGT 
GTGGAGAGCG CCACCCGTGG TCCATGCAGG TTCAACTCTC GAGAGTTGGC ACTCAGGGCT 
AATGCAACAT GTGTGGACCT TCTTGTGTGG GCAGCTAATG ATGAAACAGG TGCTGATAGT 
CTTATTTCAA GGCTGACTGA GAAACTGCAT TCCAGTGCTG GCAGCAAGCA ACTCATAGCT 
GATATGCCTC TGCTTCTGTG CTCACTGCAG GGTTTGGGCA CTCTAGCAGA GAAGTTTCCG 
ACGGTCGCTC ACGCCACCAT CGGCTCCCTC CGCGACTTTC TGATGGGGCC GTCCCGCATC 
ATCGTCAAGC TCCACAAGCA TCATGAAGTC GAGAGCAGCA AGTCTAGTGC CGGACCCACC 
ATCAGGGTCA TGGACTCCAC CCTCCAGAGG GCTGGGGCTG GGAAGGATGG GCGCGGTGCT 
GGTGGTAGTG GTGGTGCCGG TAGTGGTGGT GGGAGTAGGA GCAGCAAGAG TGTCGGGGAG 
GGGTCACATC CGTATGAGAG ACTGAGGAAT GCGGCCATGT CTAACCTTTG CAGATCGCTG 
CGTTCTGGCC TCGTTGAAGA TCCCGATTGC ATTCAAGCCT TCCTAGCGTC TGTCTCCAAT 
GCGCTCTACA TTGCCGAAGC CAGCGATCGA GATTCCACCC TCATCTCCTC CAACGCCATC 
CTGGCGATGG GCCACGTGGC AGTTCTTTTG AGCGAAACAA CTCGTACCAT TGAGAGTGTC 
CAGCAGATTT TCCAGCAACG GTTCTGCACA CCCCCTTCCT CCCTCGATGT GCTGATCGTG 
GACATGCTGG GATGCCTGGT ACTGGCTGGA AATGCTGCTG TAACGCAGGA GGTGATGAAT 
ATGTTTATTC AGATCAGTGT CGAAGCTGGA TCATCTGATT ATGCACCTAA CAGCTCACAG 
GCTTCACAGA GATTCAGGCA TTGCTCCATG GCTATCATCA ATGCTCTTGC AAACATAGCA 
GCATTTATAG ACGGAGAGAA CGAACAGCAG GAGCTCTTAG TCCGGCTACT TGAATTATTT 
GTCCAGCTGG GACTAGAGGG CAAGCGGGCC AGCGAGAGGG CTCCTACAGC TATCAAGGCC 
TCCAGCAGTG CTGGAAGCTT GGGCATCCTT ATTCCTGTCC TTGCAACTTT GATCAAGAGA 
CTGCCACCCA TCCAGGACCC AAAGCCTCGC CTTCACAAGT TCTTCAGGGA TTTCTGGCTT 
TACTGTGTGG TCATGGGGTT TGCCGTGGAG GACTCTGGTA TCAAGATTTG GAATATTGTA 
CCTCAAACTA CACGCCGTCT CTGGCCCAAA GAGTGGTACG AGGGAGTCTG TCACATCGCT 
GCAAAGTCTC CCCTCCTTAC ATCCAGCCCT GGTGAACATC TGAGATCAGA GCTACAGTAC 
AACTCTGCTC TCAGGAACGA TAGCATGACA TATAACGACC TGAACGAGCT CCGTCAGAAC 
ATCCTGGGCC TGATGGAGCA TTCCCCAGAG GTCACCGTTA TCGTCAACAA GCTCAATTTT 
GCCCAGTGTA CCTACCTGCT CTCTGTCCAT CGGCTGGAGA CCCTGCGGGT GAGTAACGCC 
GTCCCCACCA GCTTCGCAGC AGTCTTCAGC TACCTGGAAG ACAAGGCGAT TATCAAGGAC 
AAGGCTGGCA TGTGGCAGTG TCTCCTGGCA TCCTTCGACA AGGTCTTCGA CATGTTCTTG 
GATATCAAGG CCAAAATGCC CCGTACTGAA GAGAGGGAGA AGGAGCTGGT CCATCATGCT 
CAGTTCCTGC TTGTCAAGTT CAATCATGTC CTCAAACAGA TCAGGAAGGT TGCTGATAAA 
TACCTTTCAA GACTTGTAGA CAGGTTTCCT CAGTTACTGT GGAATGGTGA TGTCCTTTGG 
AGCATGTTGG ATCTCCTGCA GCTCTTGTCC AAGGCTGCTG CAGAGGAGAG TCTTCAGGAG 
GTCAGAGAGT TCCCTGTTTT CAAGGGCATG TTCATGCTGA CAGTCATGGA CACCAAGGAA 
TCAAGAGAGG GCATTGTCCG TGACTTTGCA GCCCGTTGCG GAGGTATTTT CCAGGAGGCC 
GTGAAGTGGG CGGCCTCTGC GACGCGCTCT CACATCCAGC AGTACATCAT CCATCTGGAG 
AACTCCTCGG ACAGTCTCTC ACAGCGTAGC ACCCTGGAGC TCGCCACGGA GAGTGCCCTC 
CAGTGTGCAG GATTCAACAA AGGACTCACC GCATCTGGTA CCGACAGTTT GACCAGTGAG 
TCAGCCAACT TTGTATCAGT GATGAGCCTA AGGAGCAGGT TCGCTGGAGA GGTATCAGGC 
ATGAAAGCAA TCTACACCGA CGCAGGGCCC GGGGGTTTGA GTCTATCTCA TGTACTGAAT 
AAACAGCTTG ACAAGGCGTG TGATGCGAAG AAGATAGATG CTTTCACTAA TTCTATGTTC 
AGAGTCTGTG CCCTCCTCAT CTCAAGTGAA GGTGTGGATA GATACCTGTT GTATAGCCTG 
TGCAGGGCGC CTGTCAGGCT CTTCACAACA CACGCTATGG AGAAGGCTGT TGCATGCTGG 
GAATGGCTGC TGGCTGCCAG ATCGGACATG GAACTGCAGT TAATGCGTGA GATGGCCGGA 
GCATGGCAGA TGAGCATGGA CAACAGACTT GGTCTGTTCT CTATTGATGT TGAAGATCCC 
AGTCCTCTGG CCGCCATCAG TGGCGATGAT GTCCCTGACC CCAAACACCC AAACGCTACA 
CCACATCACA TCTGGACCCA GTTCTTACAC CAGCGCCTGG AGAGCATCAA GTACAGCAGC 
ATCAACGTGG TGGAGATCTT CATCTCCATC CTGCATCGCT CCCTACCCAT GCAGGTCGGT 
CAGACAAAGG GCGCCCTCTC CAGGCACATC GCTGCCATGG GCCCCCGTTT CCGCCTGCTC 
TGTATGGGAC TGTCCCTCCT GCAGGGGGAT ATCCTGCCCA GCAGTACGGG CAAGAACGTC 
TTGAGAGAAA GGATCTACTC GGCTGCGTTC GACTTCTTCA GTGTTGCTCC AGTGTGCCCA 
ACCCAGTCTG TGAGTGAGCT GAGGGAAGAC ATCAGTATTC TCATTAAGTT CTTCCATGCC 
ATGCTGGCAG AGAAGAAATA CCTCAGAGGC ACCACAGCTG TGGTTCAAGA GTCCGAGGAT 
GTACACACGC TAGGCATCAT CCCAGCCGAC CTGCGAGCCA GTAACGAGTA CCTCAACGCC 
AGGCCAACAG CAGGGCGCCC TCAGGGTTGG ATGAACACCA TGCCGCTGTC CAGTAACATG 
TCCATCGTGT CCAAGAGGTC ATCGGTCACC AGGAAGAGTG GAGAGGTCGG AGCAAAGTAT 
GCCAAGGAGT ACATGAAGAG GAGGAATCTA ATCCTCTCTC TGCTGGTGAA TGAGATAGAG 
AGACTGTGTT GCTGGTGTAA TCCCTTAGAC AACTCAGAAA TCAAGCTACC AGGAGAGGAG 
CTGGTTGCTA ACTGGAGGGT CCAGACCCCT ACCAATGAAC GCACCTGGAG GGACTTTGCT 
CACCTCGCCT GGGAGGTGTC CCCTCATCTG GCTTGTCAGC TTCCCGTCAG GTTCAAGTCT 
TCTGAGAGCC TTGTCAAGGA GGTGACGCGT ATGGTGCGAC TCAACCCCAT GGCTGTCTCT 
GACCTCCCAA GTGCCATTCA CTTCCTGGTC ACAGAACACA GTGTGGATGC AGATGTCGCA 
GAGTTGAGTC ACATCCTATG CTGGGCGCCC GTTCCACCTG TCACCGCCCT GTCATACTTC 
TCCCAGCAGT ACCCCCCGCA CCCCCTGACT GCCCAGTATG CCACCAGAGT CCTGCAGGAC 
TACCCACCAG AGGCACTGCT CTTCTATGTC CCTCAGCTAG TACAGGCTGT CAGATACGAT 
ACGTTTGGCT TTGTGACGGA GCTGATCCTG GTCCTGGCGA AAAAGTCCCA ACTCCTGGCC 
CATCAGCTCA TCTGGAACAT GAAGACCAAC ATCTTCAAAG ACGAGGAAGC CAAGGAAAGA 
GACGGTGAGT TTGCTGATGC CCTGGAGGCC CTGATCGAAG GCATCATAGC CAGCTTGTCG 
GGACCAGCGA AAGACTTCTA TGAGAGGGAG TTTGACTTTT TTGGCAAAAT AACAGCCATC 
TCCGGCGAGA TCAGACCATA TCCCAAGGGT GATGAAAGGA AAGCAGCCTG TCTGAAAGCC 
CTAGCAACCA TTCAACTCCA ACCTGGATGC TATCTCCCTT GCAACCCTGA AGCCGTCGTC 
ATGGAAATCG ACTACAAGTC TGGAACTCCC ATGCAGAGTG CTGCCAAGGC CCCGTTCCTT 
GCCAGGTTCA AGGTGCAGAA GTGCGGAGTT CATGGGCTGG AGAGCCTTGG TATGAAGGGC 
CTCGATTCAG ACGCTGTTTC TCGTTCCCTC AAAGCACAAA TATGGGAGGC TTGCATCTTT 
AAGGTTGGAG ATGACGTGAG ACAGGACATG CTTGCGCTGC AGGTGATTGG TCTATTCAAG 
AACATCTTCC ATCAGGTGGG ACTCAACCTC TACCTGGTCC CGTACAAAGT GGTAGCAACG 
TCACCAGGGT GTGGAGTGAT CCAGTGTGTC CCAGACTGCA AGTCTCGTGA CCAGCTTGGC 
CGACAGACAG ACATCGGCAT GTATGAATAC TTCCTAACCA AGTATGGAGA TGAGACTACA 
CCCGCATACC AGAAGGCTAG ACGTCATTTC ATCCAAAGCA TGGCAGCCTA CAGTGTGGTA 
GGGCACCTCC TCCAAATCAA AGACAGACAT AATGGAAATA TCATGCTGGA TACCAGAGGC 
AACATCATAC ACATTGATTT TGGTTTCATG TTTGAGAGTT CTCCTGGAGG CAACCTGGGC 
TGGGAACCAG ACATCAAGCT CACTGATGAA ATGCTCATGA TCATGGGAGG CAATATAAAC 
GCGGCTCCAT TCAAGAAGTT TGAAGAACTG TGTGTACAGG CCTACCTAGC AGTCAGACCA 
TACCAAGAAG CCATTGTGTC CCTTGTAGCC CTGATGCTTG ATACAGGACT ACCTTGCTTC 
AAAGGAAAAA CACTCAACTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030847458.1
CDS	41..6184
Translation	MGPNGLFPETLLHLARSLAQIPDLKWLKVQQLLQMCPQIPAVGGGLCRIDQRRQDAMVALGVFLLESDLKFKDKLVPYFISLVRSIPNAQWIEETGYRVIDVLPMSERFAFTITTLLSDIATKDSHYRDQILSTLVEVLQALCRICQQPSEHPQVAICKHVMPAFLGMSRAIGRSSQDPSCSLLSRMYPSSKHTASQTPPPSSGLDQTSPMYSSFRLIVSDVMEQKLRQSEGSRQDALPLADDSREERATEGPSHLLTVVGSSFGISTETCESGSDGAQVGSLDLTTPQLQTILNVCQKMISGTLLQSLDGMAQQYALQPGSKKNLPYKSYSEVINVVLVSLLRHLLQQHKGLPMGFQTEVHAFVRELFLSGQTELQDLSVESATRGPCRFNSRELALRANATCVDLLVWAANDETGADSLISRLTEKLHSSAGSKQLIADMPLLLCSLQGLGTLAEKFPTVAHATIGSLRDFLMGPSRIIVKLHKHHEVESSKSSAGPTIRVMDSTLQRAGAGKDGRGAGGSGGAGSGGGSRSSKSVGEGSHPYERLRNAAMSNLCRSLRSGLVEDPDCIQAFLASVSNALYIAEASDRDSTLISSNAILAMGHVAVLLSETTRTIESVQQIFQQRFCTPPSSLDVLIVDMLGCLVLAGNAAVTQEVMNMFIQISVEAGSSDYAPNSSQASQRFRHCSMAIINALANIAAFIDGENEQQELLVRLLELFVQLGLEGKRASERAPTAIKASSSAGSLGILIPVLATLIKRLPPIQDPKPRLHKFFRDFWLYCVVMGFAVEDSGIKIWNIVPQTTRRLWPKEWYEGVCHIAAKSPLLTSSPGEHLRSELQYNSALRNDSMTYNDLNELRQNILGLMEHSPEVTVIVNKLNFAQCTYLLSVHRLETLRVSNAVPTSFAAVFSYLEDKAIIKDKAGMWQCLLASFDKVFDMFLDIKAKMPRTEEREKELVHHAQFLLVKFNHVLKQIRKVADKYLSRLVDRFPQLLWNGDVLWSMLDLLQLLSKAAAEESLQEVREFPVFKGMFMLTVMDTKESREGIVRDFAARCGGIFQEAVKWAASATRSHIQQYIIHLENSSDSLSQRSTLELATESALQCAGFNKGLTASGTDSLTSESANFVSVMSLRSRFAGEVSGMKAIYTDAGPGGLSLSHVLNKQLDKACDAKKIDAFTNSMFRVCALLISSEGVDRYLLYSLCRAPVRLFTTHAMEKAVACWEWLLAARSDMELQLMREMAGAWQMSMDNRLGLFSIDVEDPSPLAAISGDDVPDPKHPNATPHHIWTQFLHQRLESIKYSSINVVEIFISILHRSLPMQVGQTKGALSRHIAAMGPRFRLLCMGLSLLQGDILPSSTGKNVLRERIYSAAFDFFSVAPVCPTQSVSELREDISILIKFFHAMLAEKKYLRGTTAVVQESEDVHTLGIIPADLRASNEYLNARPTAGRPQGWMNTMPLSSNMSIVSKRSSVTRKSGEVGAKYAKEYMKRRNLILSLLVNEIERLCCWCNPLDNSEIKLPGEELVANWRVQTPTNERTWRDFAHLAWEVSPHLACQLPVRFKSSESLVKEVTRMVRLNPMAVSDLPSAIHFLVTEHSVDADVAELSHILCWAPVPPVTALSYFSQQYPPHPLTAQYATRVLQDYPPEALLFYVPQLVQAVRYDTFGFVTELILVLAKKSQLLAHQLIWNMKTNIFKDEEAKERDGEFADALEALIEGIIASLSGPAKDFYEREFDFFGKITAISGEIRPYPKGDERKAACLKALATIQLQPGCYLPCNPEAVVMEIDYKSGTPMQSAAKAPFLARFKVQKCGVHGLESLGMKGLDSDAVSRSLKAQIWEACIFKVGDDVRQDMLALQVIGLFKNIFHQVGLNLYLVPYKVVATSPGCGVIQCVPDCKSRDQLGRQTDIGMYEYFLTKYGDETTPAYQKARRHFIQSMAAYSVVGHLLQIKDRHNGNIMLDTRGNIIHIDFGFMFESSPGGNLGWEPDIKLTDEMLMIMGGNINAAPFKKFEELCVQAYLAVRPYQEAIVSLVALMLDTGLPCFKGKTLNY

Target ORF information:

RefSeq Version	XM_030991598.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus phosphatidylinositol 4-kinase alpha (LOC590935), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030991598.1

ORF Insert Sequence:

ATGGGGCCAA ACGGATTGTT CCCAGAAACG CTTCTACATT TAGCAAGATC ATTGGCCCAA 
ATCCCAGATC TGAAATGGCT AAAGGTGCAG CAACTGTTGC AGATGTGTCC CCAGATACCC 
GCTGTGGGAG GAGGGCTGTG CAGGATAGAC CAGCGTAGGC AGGATGCTAT GGTGGCGCTG 
GGGGTCTTCC TACTCGAATC AGACCTCAAG TTCAAGGACA AGTTGGTTCC ATATTTCATT 
AGTCTTGTGA GAAGCATACC CAATGCACAA TGGATTGAAG AAACGGGATA CAGGGTTATT 
GATGTATTAC CAATGAGTGA GCGCTTTGCA TTCACCATCA CAACTCTCCT TTCTGACATA 
GCGACCAAAG ACTCACACTA CAGAGATCAG ATTTTGTCAA CATTAGTTGA AGTTTTACAG 
GCACTATGCA GGATCTGCCA ACAGCCATCA GAACATCCTC AAGTTGCTAT ATGCAAGCAC 
GTCATGCCCG CATTCCTTGG GATGTCCCGT GCCATAGGGC GCAGCAGCCA GGACCCATCC 
TGCTCTCTCC TCAGTCGGAT GTACCCCTCA TCCAAGCACA CCGCCTCCCA GACCCCTCCC 
CCATCCTCCG GTTTGGACCA GACGTCCCCC ATGTACAGCT CCTTCCGACT CATCGTCTCC 
GACGTCATGG AGCAGAAACT CCGGCAGTCC GAAGGGAGCC GACAGGACGC ATTGCCTTTG 
GCAGACGACT CCAGGGAGGA GAGGGCGACG GAGGGACCTA GCCATCTGCT GACAGTGGTG 
GGGTCAAGTT TCGGTATCAG CACGGAGACA TGTGAGAGCG GGAGCGATGG AGCTCAAGTG 
GGTTCTCTGG ACCTCACCAC TCCACAGCTG CAGACCATCT TGAATGTGTG CCAAAAGATG 
ATCAGCGGAA CTCTGTTACA AAGTCTTGAT GGGATGGCCC AGCAGTACGC CCTTCAACCA 
GGATCAAAGA AGAACCTTCC GTACAAGTCA TATAGTGAAG TCATCAACGT GGTTCTGGTC 
TCTTTACTCA GACATCTCCT TCAACAGCAT AAAGGATTGC CAATGGGCTT CCAGACAGAG 
GTTCATGCGT TTGTCAGAGA ACTCTTTCTA TCGGGCCAAA CGGAGCTGCA GGATCTGAGT 
GTGGAGAGCG CCACCCGTGG TCCATGCAGG TTCAACTCTC GAGAGTTGGC ACTCAGGGCT 
AATGCAACAT GTGTGGACCT TCTTGTGTGG GCAGCTAATG ATGAAACAGG TGCTGATAGT 
CTTATTTCAA GGCTGACTGA GAAACTGCAT TCCAGTGCTG GCAGCAAGCA ACTCATAGCT 
GATATGCCTC TGCTTCTGTG CTCACTGCAG GGTTTGGGCA CTCTAGCAGA GAAGTTTCCG 
ACGGTCGCTC ACGCCACCAT CGGCTCCCTC CGCGACTTTC TGATGGGGCC GTCCCGCATC 
ATCGTCAAGC TCCACAAGCA TCATGAAGTC GAGAGCAGCA AGTCTAGTGC CGGACCCACC 
ATCAGGGTCA TGGACTCCAC CCTCCAGAGG GCTGGGGCTG GGAAGGATGG GCGCGGTGCT 
GGTGGTAGTG GTGGTGCCGG TAGTGGTGGT GGGAGTAGGA GCAGCAAGAG TGTCGGGGAG 
GGGTCACATC CGTATGAGAG ACTGAGGAAT GCGGCCATGT CTAACCTTTG CAGATCGCTG 
CGTTCTGGCC TCGTTGAAGA TCCCGATTGC ATTCAAGCCT TCCTAGCGTC TGTCTCCAAT 
GCGCTCTACA TTGCCGAAGC CAGCGATCGA GATTCCACCC TCATCTCCTC CAACGCCATC 
CTGGCGATGG GCCACGTGGC AGTTCTTTTG AGCGAAACAA CTCGTACCAT TGAGAGTGTC 
CAGCAGATTT TCCAGCAACG GTTCTGCACA CCCCCTTCCT CCCTCGATGT GCTGATCGTG 
GACATGCTGG GATGCCTGGT ACTGGCTGGA AATGCTGCTG TAACGCAGGA GGTGATGAAT 
ATGTTTATTC AGATCAGTGT CGAAGCTGGA TCATCTGATT ATGCACCTAA CAGCTCACAG 
GCTTCACAGA GATTCAGGCA TTGCTCCATG GCTATCATCA ATGCTCTTGC AAACATAGCA 
GCATTTATAG ACGGAGAGAA CGAACAGCAG GAGCTCTTAG TCCGGCTACT TGAATTATTT 
GTCCAGCTGG GACTAGAGGG CAAGCGGGCC AGCGAGAGGG CTCCTACAGC TATCAAGGCC 
TCCAGCAGTG CTGGAAGCTT GGGCATCCTT ATTCCTGTCC TTGCAACTTT GATCAAGAGA 
CTGCCACCCA TCCAGGACCC AAAGCCTCGC CTTCACAAGT TCTTCAGGGA TTTCTGGCTT 
TACTGTGTGG TCATGGGGTT TGCCGTGGAG GACTCTGGTA TCAAGATTTG GAATATTGTA 
CCTCAAACTA CACGCCGTCT CTGGCCCAAA GAGTGGTACG AGGGAGTCTG TCACATCGCT 
GCAAAGTCTC CCCTCCTTAC ATCCAGCCCT GGTGAACATC TGAGATCAGA GCTACAGTAC 
AACTCTGCTC TCAGGAACGA TAGCATGACA TATAACGACC TGAACGAGCT CCGTCAGAAC 
ATCCTGGGCC TGATGGAGCA TTCCCCAGAG GTCACCGTTA TCGTCAACAA GCTCAATTTT 
GCCCAGTGTA CCTACCTGCT CTCTGTCCAT CGGCTGGAGA CCCTGCGGGT GAGTAACGCC 
GTCCCCACCA GCTTCGCAGC AGTCTTCAGC TACCTGGAAG ACAAGGCGAT TATCAAGGAC 
AAGGCTGGCA TGTGGCAGTG TCTCCTGGCA TCCTTCGACA AGGTCTTCGA CATGTTCTTG 
GATATCAAGG CCAAAATGCC CCGTACTGAA GAGAGGGAGA AGGAGCTGGT CCATCATGCT 
CAGTTCCTGC TTGTCAAGTT CAATCATGTC CTCAAACAGA TCAGGAAGGT TGCTGATAAA 
TACCTTTCAA GACTTGTAGA CAGGTTTCCT CAGTTACTGT GGAATGGTGA TGTCCTTTGG 
AGCATGTTGG ATCTCCTGCA GCTCTTGTCC AAGGCTGCTG CAGAGGAGAG TCTTCAGGAG 
GTCAGAGAGT TCCCTGTTTT CAAGGGCATG TTCATGCTGA CAGTCATGGA CACCAAGGAA 
TCAAGAGAGG GCATTGTCCG TGACTTTGCA GCCCGTTGCG GAGGTATTTT CCAGGAGGCC 
GTGAAGTGGG CGGCCTCTGC GACGCGCTCT CACATCCAGC AGTACATCAT CCATCTGGAG 
AACTCCTCGG ACAGTCTCTC ACAGCGTAGC ACCCTGGAGC TCGCCACGGA GAGTGCCCTC 
CAGTGTGCAG GATTCAACAA AGGACTCACC GCATCTGGTA CCGACAGTTT GACCAGTGAG 
TCAGCCAACT TTGTATCAGT GATGAGCCTA AGGAGCAGGT TCGCTGGAGA GGTATCAGGC 
ATGAAAGCAA TCTACACCGA CGCAGGGCCC GGGGGTTTGA GTCTATCTCA TGTACTGAAT 
AAACAGCTTG ACAAGGCGTG TGATGCGAAG AAGATAGATG CTTTCACTAA TTCTATGTTC 
AGAGTCTGTG CCCTCCTCAT CTCAAGTGAA GGTGTGGATA GATACCTGTT GTATAGCCTG 
TGCAGGGCGC CTGTCAGGCT CTTCACAACA CACGCTATGG AGAAGGCTGT TGCATGCTGG 
GAATGGCTGC TGGCTGCCAG ATCGGACATG GAACTGCAGT TAATGCGTGA GATGGCCGGA 
GCATGGCAGA TGAGCATGGA CAACAGACTT GGTCTGTTCT CTATTGATGT TGAAGATCCC 
AGTCCTCTGG CCGCCATCAG TGGCGATGAT GTCCCTGACC CCAAACACCC AAACGCTACA 
CCACATCACA TCTGGACCCA GTTCTTACAC CAGCGCCTGG AGAGCATCAA GTACAGCAGC 
ATCAACGTGG TGGAGATCTT CATCTCCATC CTGCATCGCT CCCTACCCAT GCAGGTCGGT 
CAGACAAAGG GCGCCCTCTC CAGGCACATC GCTGCCATGG GCCCCCGTTT CCGCCTGCTC 
TGTATGGGAC TGTCCCTCCT GCAGGGGGAT ATCCTGCCCA GCAGTACGGG CAAGAACGTC 
TTGAGAGAAA GGATCTACTC GGCTGCGTTC GACTTCTTCA GTGTTGCTCC AGTGTGCCCA 
ACCCAGTCTG TGAGTGAGCT GAGGGAAGAC ATCAGTATTC TCATTAAGTT CTTCCATGCC 
ATGCTGGCAG AGAAGAAATA CCTCAGAGGC ACCACAGCTG TGGTTCAAGA GTCCGAGGAT 
GTACACACGC TAGGCATCAT CCCAGCCGAC CTGCGAGCCA GTAACGAGTA CCTCAACGCC 
AGGCCAACAG CAGGGCGCCC TCAGGGTTGG ATGAACACCA TGCCGCTGTC CAGTAACATG 
TCCATCGTGT CCAAGAGGTC ATCGGTCACC AGGAAGAGTG GAGAGGTCGG AGCAAAGTAT 
GCCAAGGAGT ACATGAAGAG GAGGAATCTA ATCCTCTCTC TGCTGGTGAA TGAGATAGAG 
AGACTGTGTT GCTGGTGTAA TCCCTTAGAC AACTCAGAAA TCAAGCTACC AGGAGAGGAG 
CTGGTTGCTA ACTGGAGGGT CCAGACCCCT ACCAATGAAC GCACCTGGAG GGACTTTGCT 
CACCTCGCCT GGGAGGTGTC CCCTCATCTG GCTTGTCAGC TTCCCGTCAG GTTCAAGTCT 
TCTGAGAGCC TTGTCAAGGA GGTGACGCGT ATGGTGCGAC TCAACCCCAT GGCTGTCTCT 
GACCTCCCAA GTGCCATTCA CTTCCTGGTC ACAGAACACA GTGTGGATGC AGATGTCGCA 
GAGTTGAGTC ACATCCTATG CTGGGCGCCC GTTCCACCTG TCACCGCCCT GTCATACTTC 
TCCCAGCAGT ACCCCCCGCA CCCCCTGACT GCCCAGTATG CCACCAGAGT CCTGCAGGAC 
TACCCACCAG AGGCACTGCT CTTCTATGTC CCTCAGCTAG TACAGGCTGT CAGATACGAT 
ACGTTTGGCT TTGTGACGGA GCTGATCCTG GTCCTGGCGA AAAAGTCCCA ACTCCTGGCC 
CATCAGCTCA TCTGGAACAT GAAGACCAAC ATCTTCAAAG ACGAGGAAGC CAAGGAAAGA 
GACGGTGAGT TTGCTGATGC CCTGGAGGCC CTGATCGAAG GCATCATAGC CAGCTTGTCG 
GGACCAGCGA AAGACTTCTA TGAGAGGGAG TTTGACTTTT TTGGCAAAAT AACAGCCATC 
TCCGGCGAGA TCAGACCATA TCCCAAGGGT GATGAAAGGA AAGCAGCCTG TCTGAAAGCC 
CTAGCAACCA TTCAACTCCA ACCTGGATGC TATCTCCCTT GCAACCCTGA AGCCGTCGTC 
ATGGAAATCG ACTACAAGTC TGGAACTCCC ATGCAGAGTG CTGCCAAGGC CCCGTTCCTT 
GCCAGGTTCA AGGTGCAGAA GTGCGGAGTT CATGGGCTGG AGAGCCTTGG TATGAAGGGC 
CTCGATTCAG ACGCTGTTTC TCGTTCCCTC AAAGCACAAA TATGGGAGGC TTGCATCTTT 
AAGGTTGGAG ATGACGTGAG ACAGGACATG CTTGCGCTGC AGGTGATTGG TCTATTCAAG 
AACATCTTCC ATCAGGTGGG ACTCAACCTC TACCTGGTCC CGTACAAAGT GGTAGCAACG 
TCACCAGGGT GTGGAGTGAT CCAGTGTGTC CCAGACTGCA AGTCTCGTGA CCAGCTTGGC 
CGACAGACAG ACATCGGCAT GTATGAATAC TTCCTAACCA AGTATGGAGA TGAGACTACA 
CCCGCATACC AGAAGGCTAG ACGTCATTTC ATCCAAAGCA TGGCAGCCTA CAGTGTGGTA 
GGGCACCTCC TCCAAATCAA AGACAGACAT AATGGAAATA TCATGCTGGA TACCAGAGGC 
AACATCATAC ACATTGATTT TGGTTTCATG TTTGAGAGTT CTCCTGGAGG CAACCTGGGC 
TGGGAACCAG ACATCAAGCT CACTGATGAA ATGCTCATGA TCATGGGAGG CAATATAAAC 
GCGGCTCCAT TCAAGAAGTT TGAAGAACTG TGTGTACAGG CCTACCTAGC AGTCAGACCA 
TACCAAGAAG CCATTGTGTC CCTTGTAGCC CTGATGCTTG ATACAGGACT ACCTTGCTTC 
AAAGGAAAAA CACTCAACTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC590935 ( XM_030991599.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030847459.1 Strongylocentrotus purpuratus phosphatidylinositol 4-kinase alpha (LOC590935), transcript variant X2, mRNA.

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CloneID	OSd57604
Clone ID Related Accession (Same CDS sequence)	XM_030991599.1
Accession Version	XM_030991599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	phosphatidylinositol 4-kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145605.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCAA ACGGATTGTT CCCAGAAACG CTTCTACATT TAGCAAGATC ATTGGCCCAA 
ATCCCAGATC TGAAATGGCT AAAGGTGCAG CAACTGTTGC AGATGTGTCC CCAGATACCC 
GCTGTGGGAG GAGGGCTGTG CAGGATAGAC CAGCGTAGGC AGGATGCTAT GGTGGCGCTG 
GGGGTCTTCC TACTCGAATC AGACCTCAAG TTCAAGGACA AGTTGGTTCC ATATTTCATT 
AGTCTTGTGA GAAGCATACC CAATGCACAA TGGATTGAAG AAACGGGATA CAGGGTTATT 
GATGTATTAC CAATGAGTGA GCGCTTTGCA TTCACCATCA CAACTCTCCT TTCTGACATA 
GCGACCAAAG ACTCACACTA CAGAGATCAG ATTTTGTCAA CATTAGTTGA AGTTTTACAG 
GCACTATGCA GGATCTGCCA ACAGCCATCA GAACATCCTC AAGTTGCTAT ATGCAAGCAC 
GTCATGCCCG CATTCCTTGG GATGTCCCGT GCCATAGGGC GCAGCAGCCA GGACCCATCC 
TGCTCTCTCC TCAGTCGGAT GTACCCCTCA TCCAAGCACA CCGCCTCCCA GACCCCTCCC 
CCATCCTCCG GTTTGGACCA GACGTCCCCC ATGTACAGCT CCTTCCGACT CATCGTCTCC 
GACGTCATGG AGCAGAAACT CCGGCAGTCC GAAGGGAGCC GACAGGACGC ATTGCCTTTG 
GCAGACGACT CCAGGGAGGA GAGGGCGACG GAGGGACCTA GCCATCTGCT GACAGTGGTG 
GGGTCAAGTT TCGGTATCAG CACGGAGACA TGTGAGAGCG GGAGCGATGG AGCTCAAGTG 
GGTTCTCTGG ACCTCACCAC TCCACAGCTG CAGACCATCT TGAATGTGTG CCAAAAGATG 
ATCAGCGGAA CTCTGTTACA AAGTCTTGAT GGGATGGCCC AGCAGTACGC CCTTCAACCA 
GGATCAAAGA AGAACCTTCC GTACAAGTCA TATAGTGAAG TCATCAACGT GGTTCTGGTC 
TCTTTACTCA GACATCTCCT TCAACAGCAT AAAGGATTGC CAATGGGCTT CCAGACAGAG 
GTTCATGCGT TTGTCAGAGA ACTCTTTCTA TCGGGCCAAA CGGAGCTGCA GGATCTGAGT 
GTGGAGAGCG CCACCCGTGG TCCATGCAGG TTCAACTCTC GAGAGTTGGC ACTCAGGGCT 
AATGCAACAT GTGTGGACCT TCTTGTGTGG GCAGCTAATG ATGAAACAGG TGCTGATAGT 
CTTATTTCAA GGCTGACTGA GAAACTGCAT TCCAGTGCTG GCAGCAAGCA ACTCATAGCT 
GATATGCCTC TGCTTCTGTG CTCACTGCAG GGTTTGGGCA CTCTAGCAGA GAAGTTTCCG 
ACGGTCGCTC ACGCCACCAT CGGCTCCCTC CGCGACTTTC TGATGGGGCC GTCCCGCATC 
ATCGTCAAGC TCCACAAGCA TCATGAAGTC GAGAGCAGCA AGTCTAGTGC CGGACCCACC 
ATCAGGGTCA TGGACTCCAC CCTCCAGAGG GCTGGGGCTG GGAAGGATGG GCGCGGTGCT 
GGTGGTAGTG GTGGTGCCGG TAGTGGTGGT GGGAGTAGGA GCAGCAAGAG TGTCGGGGAG 
GGGTCACATC CGTATGAGAG ACTGAGGAAT GCGGCCATGT CTAACCTTTG CAGATCGCTG 
CGTTCTGGCC TCGTTGAAGA TCCCGATTGC ATTCAAGCCT TCCTAGCGTC TGTCTCCAAT 
GCGCTCTACA TTGCCGAAGC CAGCGATCGA GATTCCACCC TCATCTCCTC CAACGCCATC 
CTGGCGATGG GCCACGTGGC AGTTCTTTTG AGCGAAACAA CTCGTACCAT TGAGAGTGTC 
CAGCAGATTT TCCAGCAACG GTTCTGCACA CCCCCTTCCT CCCTCGATGT GCTGATCGTG 
GACATGCTGG GATGCCTGGT ACTGGCTGGA AATGCTGCTG TAACGCAGGA GGTGATGAAT 
ATGTTTATTC AGATCAGTGT CGAAGCTGGA TCATCTGATT ATGCACCTAA CAGCTCACAG 
GCTTCACAGA GATTCAGGCA TTGCTCCATG GCTATCATCA ATGCTCTTGC AAACATAGCA 
GCATTTATAG ACGGAGAGAA CGAACAGCAG GAGCTCTTAG TCCGGCTACT TGAATTATTT 
GTCCAGCTGG GACTAGAGGG CAAGCGGGCC AGCGAGAGGG CTCCTACAGC TATCAAGGCC 
TCCAGCAGTG CTGGAAGCTT GGGCATCCTT ATTCCTGTCC TTGCAACTTT GATCAAGAGA 
CTGCCACCCA TCCAGGACCC AAAGCCTCGC CTTCACAAGT TCTTCAGGGA TTTCTGGCTT 
TACTGTGTGG TCATGGGGTT TGCCGTGGAG GACTCTGGTC TCTGGCCCAA AGAGTGGTAC 
GAGGGAGTCT GTCACATCGC TGCAAAGTCT CCCCTCCTTA CATCCAGCCC TGGTGAACAT 
CTGAGATCAG AGCTACAGTA CAACTCTGCT CTCAGGAACG ATAGCATGAC ATATAACGAC 
CTGAACGAGC TCCGTCAGAA CATCCTGGGC CTGATGGAGC ATTCCCCAGA GGTCACCGTT 
ATCGTCAACA AGCTCAATTT TGCCCAGTGT ACCTACCTGC TCTCTGTCCA TCGGCTGGAG 
ACCCTGCGGG TGAGTAACGC CGTCCCCACC AGCTTCGCAG CAGTCTTCAG CTACCTGGAA 
GACAAGGCGA TTATCAAGGA CAAGGCTGGC ATGTGGCAGT GTCTCCTGGC ATCCTTCGAC 
AAGGTCTTCG ACATGTTCTT GGATATCAAG GCCAAAATGC CCCGTACTGA AGAGAGGGAG 
AAGGAGCTGG TCCATCATGC TCAGTTCCTG CTTGTCAAGT TCAATCATGT CCTCAAACAG 
ATCAGGAAGG TTGCTGATAA ATACCTTTCA AGACTTGTAG ACAGGTTTCC TCAGTTACTG 
TGGAATGGTG ATGTCCTTTG GAGCATGTTG GATCTCCTGC AGCTCTTGTC CAAGGCTGCT 
GCAGAGGAGA GTCTTCAGGA GGTCAGAGAG TTCCCTGTTT TCAAGGGCAT GTTCATGCTG 
ACAGTCATGG ACACCAAGGA ATCAAGAGAG GGCATTGTCC GTGACTTTGC AGCCCGTTGC 
GGAGGTATTT TCCAGGAGGC CGTGAAGTGG GCGGCCTCTG CGACGCGCTC TCACATCCAG 
CAGTACATCA TCCATCTGGA GAACTCCTCG GACAGTCTCT CACAGCGTAG CACCCTGGAG 
CTCGCCACGG AGAGTGCCCT CCAGTGTGCA GGATTCAACA AAGGACTCAC CGCATCTGGT 
ACCGACAGTT TGACCAGTGA GTCAGCCAAC TTTGTATCAG TGATGAGCCT AAGGAGCAGG 
TTCGCTGGAG AGGTATCAGG CATGAAAGCA ATCTACACCG ACGCAGGGCC CGGGGGTTTG 
AGTCTATCTC ATGTACTGAA TAAACAGCTT GACAAGGCGT GTGATGCGAA GAAGATAGAT 
GCTTTCACTA ATTCTATGTT CAGAGTCTGT GCCCTCCTCA TCTCAAGTGA AGGTGTGGAT 
AGATACCTGT TGTATAGCCT GTGCAGGGCG CCTGTCAGGC TCTTCACAAC ACACGCTATG 
GAGAAGGCTG TTGCATGCTG GGAATGGCTG CTGGCTGCCA GATCGGACAT GGAACTGCAG 
TTAATGCGTG AGATGGCCGG AGCATGGCAG ATGAGCATGG ACAACAGACT TGGTCTGTTC 
TCTATTGATG TTGAAGATCC CAGTCCTCTG GCCGCCATCA GTGGCGATGA TGTCCCTGAC 
CCCAAACACC CAAACGCTAC ACCACATCAC ATCTGGACCC AGTTCTTACA CCAGCGCCTG 
GAGAGCATCA AGTACAGCAG CATCAACGTG GTGGAGATCT TCATCTCCAT CCTGCATCGC 
TCCCTACCCA TGCAGGTCGG TCAGACAAAG GGCGCCCTCT CCAGGCACAT CGCTGCCATG 
GGCCCCCGTT TCCGCCTGCT CTGTATGGGA CTGTCCCTCC TGCAGGGGGA TATCCTGCCC 
AGCAGTACGG GCAAGAACGT CTTGAGAGAA AGGATCTACT CGGCTGCGTT CGACTTCTTC 
AGTGTTGCTC CAGTGTGCCC AACCCAGTCT GTGAGTGAGC TGAGGGAAGA CATCAGTATT 
CTCATTAAGT TCTTCCATGC CATGCTGGCA GAGAAGAAAT ACCTCAGAGG CACCACAGCT 
GTGGTTCAAG AGTCCGAGGA TGTACACACG CTAGGCATCA TCCCAGCCGA CCTGCGAGCC 
AGTAACGAGT ACCTCAACGC CAGGCCAACA GCAGGGCGCC CTCAGGGTTG GATGAACACC 
ATGCCGCTGT CCAGTAACAT GTCCATCGTG TCCAAGAGGT CATCGGTCAC CAGGAAGAGT 
GGAGAGGTCG GAGCAAAGTA TGCCAAGGAG TACATGAAGA GGAGGAATCT AATCCTCTCT 
CTGCTGGTGA ATGAGATAGA GAGACTGTGT TGCTGGTGTA ATCCCTTAGA CAACTCAGAA 
ATCAAGCTAC CAGGAGAGGA GCTGGTTGCT AACTGGAGGG TCCAGACCCC TACCAATGAA 
CGCACCTGGA GGGACTTTGC TCACCTCGCC TGGGAGGTGT CCCCTCATCT GGCTTGTCAG 
CTTCCCGTCA GGTTCAAGTC TTCTGAGAGC CTTGTCAAGG AGGTGACGCG TATGGTGCGA 
CTCAACCCCA TGGCTGTCTC TGACCTCCCA AGTGCCATTC ACTTCCTGGT CACAGAACAC 
AGTGTGGATG CAGATGTCGC AGAGTTGAGT CACATCCTAT GCTGGGCGCC CGTTCCACCT 
GTCACCGCCC TGTCATACTT CTCCCAGCAG TACCCCCCGC ACCCCCTGAC TGCCCAGTAT 
GCCACCAGAG TCCTGCAGGA CTACCCACCA GAGGCACTGC TCTTCTATGT CCCTCAGCTA 
GTACAGGCTG TCAGATACGA TACGTTTGGC TTTGTGACGG AGCTGATCCT GGTCCTGGCG 
AAAAAGTCCC AACTCCTGGC CCATCAGCTC ATCTGGAACA TGAAGACCAA CATCTTCAAA 
GACGAGGAAG CCAAGGAAAG AGACGGTGAG TTTGCTGATG CCCTGGAGGC CCTGATCGAA 
GGCATCATAG CCAGCTTGTC GGGACCAGCG AAAGACTTCT ATGAGAGGGA GTTTGACTTT 
TTTGGCAAAA TAACAGCCAT CTCCGGCGAG ATCAGACCAT ATCCCAAGGG TGATGAAAGG 
AAAGCAGCCT GTCTGAAAGC CCTAGCAACC ATTCAACTCC AACCTGGATG CTATCTCCCT 
TGCAACCCTG AAGCCGTCGT CATGGAAATC GACTACAAGT CTGGAACTCC CATGCAGAGT 
GCTGCCAAGG CCCCGTTCCT TGCCAGGTTC AAGGTGCAGA AGTGCGGAGT TCATGGGCTG 
GAGAGCCTTG GTATGAAGGG CCTCGATTCA GACGCTGTTT CTCGTTCCCT CAAAGCACAA 
ATATGGGAGG CTTGCATCTT TAAGGTTGGA GATGACGTGA GACAGGACAT GCTTGCGCTG 
CAGGTGATTG GTCTATTCAA GAACATCTTC CATCAGGTGG GACTCAACCT CTACCTGGTC 
CCGTACAAAG TGGTAGCAAC GTCACCAGGG TGTGGAGTGA TCCAGTGTGT CCCAGACTGC 
AAGTCTCGTG ACCAGCTTGG CCGACAGACA GACATCGGCA TGTATGAATA CTTCCTAACC 
AAGTATGGAG ATGAGACTAC ACCCGCATAC CAGAAGGCTA GACGTCATTT CATCCAAAGC 
ATGGCAGCCT ACAGTGTGGT AGGGCACCTC CTCCAAATCA AAGACAGACA TAATGGAAAT 
ATCATGCTGG ATACCAGAGG CAACATCATA CACATTGATT TTGGTTTCAT GTTTGAGAGT 
TCTCCTGGAG GCAACCTGGG CTGGGAACCA GACATCAAGC TCACTGATGA AATGCTCATG 
ATCATGGGAG GCAATATAAA CGCGGCTCCA TTCAAGAAGT TTGAAGAACT GTGTGTACAG 
GCCTACCTAG CAGTCAGACC ATACCAAGAA GCCATTGTGT CCCTTGTAGC CCTGATGCTT 
GATACAGGAC TACCTTGCTT CAAAGGAAAA ACACTCAACT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030847459.1
CDS	41..6145
Translation	MGPNGLFPETLLHLARSLAQIPDLKWLKVQQLLQMCPQIPAVGGGLCRIDQRRQDAMVALGVFLLESDLKFKDKLVPYFISLVRSIPNAQWIEETGYRVIDVLPMSERFAFTITTLLSDIATKDSHYRDQILSTLVEVLQALCRICQQPSEHPQVAICKHVMPAFLGMSRAIGRSSQDPSCSLLSRMYPSSKHTASQTPPPSSGLDQTSPMYSSFRLIVSDVMEQKLRQSEGSRQDALPLADDSREERATEGPSHLLTVVGSSFGISTETCESGSDGAQVGSLDLTTPQLQTILNVCQKMISGTLLQSLDGMAQQYALQPGSKKNLPYKSYSEVINVVLVSLLRHLLQQHKGLPMGFQTEVHAFVRELFLSGQTELQDLSVESATRGPCRFNSRELALRANATCVDLLVWAANDETGADSLISRLTEKLHSSAGSKQLIADMPLLLCSLQGLGTLAEKFPTVAHATIGSLRDFLMGPSRIIVKLHKHHEVESSKSSAGPTIRVMDSTLQRAGAGKDGRGAGGSGGAGSGGGSRSSKSVGEGSHPYERLRNAAMSNLCRSLRSGLVEDPDCIQAFLASVSNALYIAEASDRDSTLISSNAILAMGHVAVLLSETTRTIESVQQIFQQRFCTPPSSLDVLIVDMLGCLVLAGNAAVTQEVMNMFIQISVEAGSSDYAPNSSQASQRFRHCSMAIINALANIAAFIDGENEQQELLVRLLELFVQLGLEGKRASERAPTAIKASSSAGSLGILIPVLATLIKRLPPIQDPKPRLHKFFRDFWLYCVVMGFAVEDSGLWPKEWYEGVCHIAAKSPLLTSSPGEHLRSELQYNSALRNDSMTYNDLNELRQNILGLMEHSPEVTVIVNKLNFAQCTYLLSVHRLETLRVSNAVPTSFAAVFSYLEDKAIIKDKAGMWQCLLASFDKVFDMFLDIKAKMPRTEEREKELVHHAQFLLVKFNHVLKQIRKVADKYLSRLVDRFPQLLWNGDVLWSMLDLLQLLSKAAAEESLQEVREFPVFKGMFMLTVMDTKESREGIVRDFAARCGGIFQEAVKWAASATRSHIQQYIIHLENSSDSLSQRSTLELATESALQCAGFNKGLTASGTDSLTSESANFVSVMSLRSRFAGEVSGMKAIYTDAGPGGLSLSHVLNKQLDKACDAKKIDAFTNSMFRVCALLISSEGVDRYLLYSLCRAPVRLFTTHAMEKAVACWEWLLAARSDMELQLMREMAGAWQMSMDNRLGLFSIDVEDPSPLAAISGDDVPDPKHPNATPHHIWTQFLHQRLESIKYSSINVVEIFISILHRSLPMQVGQTKGALSRHIAAMGPRFRLLCMGLSLLQGDILPSSTGKNVLRERIYSAAFDFFSVAPVCPTQSVSELREDISILIKFFHAMLAEKKYLRGTTAVVQESEDVHTLGIIPADLRASNEYLNARPTAGRPQGWMNTMPLSSNMSIVSKRSSVTRKSGEVGAKYAKEYMKRRNLILSLLVNEIERLCCWCNPLDNSEIKLPGEELVANWRVQTPTNERTWRDFAHLAWEVSPHLACQLPVRFKSSESLVKEVTRMVRLNPMAVSDLPSAIHFLVTEHSVDADVAELSHILCWAPVPPVTALSYFSQQYPPHPLTAQYATRVLQDYPPEALLFYVPQLVQAVRYDTFGFVTELILVLAKKSQLLAHQLIWNMKTNIFKDEEAKERDGEFADALEALIEGIIASLSGPAKDFYEREFDFFGKITAISGEIRPYPKGDERKAACLKALATIQLQPGCYLPCNPEAVVMEIDYKSGTPMQSAAKAPFLARFKVQKCGVHGLESLGMKGLDSDAVSRSLKAQIWEACIFKVGDDVRQDMLALQVIGLFKNIFHQVGLNLYLVPYKVVATSPGCGVIQCVPDCKSRDQLGRQTDIGMYEYFLTKYGDETTPAYQKARRHFIQSMAAYSVVGHLLQIKDRHNGNIMLDTRGNIIHIDFGFMFESSPGGNLGWEPDIKLTDEMLMIMGGNINAAPFKKFEELCVQAYLAVRPYQEAIVSLVALMLDTGLPCFKGKTLNY

Target ORF information:

RefSeq Version	XM_030991599.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus phosphatidylinositol 4-kinase alpha (LOC590935), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030991599.1

ORF Insert Sequence:

ATGGGGCCAA ACGGATTGTT CCCAGAAACG CTTCTACATT TAGCAAGATC ATTGGCCCAA 
ATCCCAGATC TGAAATGGCT AAAGGTGCAG CAACTGTTGC AGATGTGTCC CCAGATACCC 
GCTGTGGGAG GAGGGCTGTG CAGGATAGAC CAGCGTAGGC AGGATGCTAT GGTGGCGCTG 
GGGGTCTTCC TACTCGAATC AGACCTCAAG TTCAAGGACA AGTTGGTTCC ATATTTCATT 
AGTCTTGTGA GAAGCATACC CAATGCACAA TGGATTGAAG AAACGGGATA CAGGGTTATT 
GATGTATTAC CAATGAGTGA GCGCTTTGCA TTCACCATCA CAACTCTCCT TTCTGACATA 
GCGACCAAAG ACTCACACTA CAGAGATCAG ATTTTGTCAA CATTAGTTGA AGTTTTACAG 
GCACTATGCA GGATCTGCCA ACAGCCATCA GAACATCCTC AAGTTGCTAT ATGCAAGCAC 
GTCATGCCCG CATTCCTTGG GATGTCCCGT GCCATAGGGC GCAGCAGCCA GGACCCATCC 
TGCTCTCTCC TCAGTCGGAT GTACCCCTCA TCCAAGCACA CCGCCTCCCA GACCCCTCCC 
CCATCCTCCG GTTTGGACCA GACGTCCCCC ATGTACAGCT CCTTCCGACT CATCGTCTCC 
GACGTCATGG AGCAGAAACT CCGGCAGTCC GAAGGGAGCC GACAGGACGC ATTGCCTTTG 
GCAGACGACT CCAGGGAGGA GAGGGCGACG GAGGGACCTA GCCATCTGCT GACAGTGGTG 
GGGTCAAGTT TCGGTATCAG CACGGAGACA TGTGAGAGCG GGAGCGATGG AGCTCAAGTG 
GGTTCTCTGG ACCTCACCAC TCCACAGCTG CAGACCATCT TGAATGTGTG CCAAAAGATG 
ATCAGCGGAA CTCTGTTACA AAGTCTTGAT GGGATGGCCC AGCAGTACGC CCTTCAACCA 
GGATCAAAGA AGAACCTTCC GTACAAGTCA TATAGTGAAG TCATCAACGT GGTTCTGGTC 
TCTTTACTCA GACATCTCCT TCAACAGCAT AAAGGATTGC CAATGGGCTT CCAGACAGAG 
GTTCATGCGT TTGTCAGAGA ACTCTTTCTA TCGGGCCAAA CGGAGCTGCA GGATCTGAGT 
GTGGAGAGCG CCACCCGTGG TCCATGCAGG TTCAACTCTC GAGAGTTGGC ACTCAGGGCT 
AATGCAACAT GTGTGGACCT TCTTGTGTGG GCAGCTAATG ATGAAACAGG TGCTGATAGT 
CTTATTTCAA GGCTGACTGA GAAACTGCAT TCCAGTGCTG GCAGCAAGCA ACTCATAGCT 
GATATGCCTC TGCTTCTGTG CTCACTGCAG GGTTTGGGCA CTCTAGCAGA GAAGTTTCCG 
ACGGTCGCTC ACGCCACCAT CGGCTCCCTC CGCGACTTTC TGATGGGGCC GTCCCGCATC 
ATCGTCAAGC TCCACAAGCA TCATGAAGTC GAGAGCAGCA AGTCTAGTGC CGGACCCACC 
ATCAGGGTCA TGGACTCCAC CCTCCAGAGG GCTGGGGCTG GGAAGGATGG GCGCGGTGCT 
GGTGGTAGTG GTGGTGCCGG TAGTGGTGGT GGGAGTAGGA GCAGCAAGAG TGTCGGGGAG 
GGGTCACATC CGTATGAGAG ACTGAGGAAT GCGGCCATGT CTAACCTTTG CAGATCGCTG 
CGTTCTGGCC TCGTTGAAGA TCCCGATTGC ATTCAAGCCT TCCTAGCGTC TGTCTCCAAT 
GCGCTCTACA TTGCCGAAGC CAGCGATCGA GATTCCACCC TCATCTCCTC CAACGCCATC 
CTGGCGATGG GCCACGTGGC AGTTCTTTTG AGCGAAACAA CTCGTACCAT TGAGAGTGTC 
CAGCAGATTT TCCAGCAACG GTTCTGCACA CCCCCTTCCT CCCTCGATGT GCTGATCGTG 
GACATGCTGG GATGCCTGGT ACTGGCTGGA AATGCTGCTG TAACGCAGGA GGTGATGAAT 
ATGTTTATTC AGATCAGTGT CGAAGCTGGA TCATCTGATT ATGCACCTAA CAGCTCACAG 
GCTTCACAGA GATTCAGGCA TTGCTCCATG GCTATCATCA ATGCTCTTGC AAACATAGCA 
GCATTTATAG ACGGAGAGAA CGAACAGCAG GAGCTCTTAG TCCGGCTACT TGAATTATTT 
GTCCAGCTGG GACTAGAGGG CAAGCGGGCC AGCGAGAGGG CTCCTACAGC TATCAAGGCC 
TCCAGCAGTG CTGGAAGCTT GGGCATCCTT ATTCCTGTCC TTGCAACTTT GATCAAGAGA 
CTGCCACCCA TCCAGGACCC AAAGCCTCGC CTTCACAAGT TCTTCAGGGA TTTCTGGCTT 
TACTGTGTGG TCATGGGGTT TGCCGTGGAG GACTCTGGTC TCTGGCCCAA AGAGTGGTAC 
GAGGGAGTCT GTCACATCGC TGCAAAGTCT CCCCTCCTTA CATCCAGCCC TGGTGAACAT 
CTGAGATCAG AGCTACAGTA CAACTCTGCT CTCAGGAACG ATAGCATGAC ATATAACGAC 
CTGAACGAGC TCCGTCAGAA CATCCTGGGC CTGATGGAGC ATTCCCCAGA GGTCACCGTT 
ATCGTCAACA AGCTCAATTT TGCCCAGTGT ACCTACCTGC TCTCTGTCCA TCGGCTGGAG 
ACCCTGCGGG TGAGTAACGC CGTCCCCACC AGCTTCGCAG CAGTCTTCAG CTACCTGGAA 
GACAAGGCGA TTATCAAGGA CAAGGCTGGC ATGTGGCAGT GTCTCCTGGC ATCCTTCGAC 
AAGGTCTTCG ACATGTTCTT GGATATCAAG GCCAAAATGC CCCGTACTGA AGAGAGGGAG 
AAGGAGCTGG TCCATCATGC TCAGTTCCTG CTTGTCAAGT TCAATCATGT CCTCAAACAG 
ATCAGGAAGG TTGCTGATAA ATACCTTTCA AGACTTGTAG ACAGGTTTCC TCAGTTACTG 
TGGAATGGTG ATGTCCTTTG GAGCATGTTG GATCTCCTGC AGCTCTTGTC CAAGGCTGCT 
GCAGAGGAGA GTCTTCAGGA GGTCAGAGAG TTCCCTGTTT TCAAGGGCAT GTTCATGCTG 
ACAGTCATGG ACACCAAGGA ATCAAGAGAG GGCATTGTCC GTGACTTTGC AGCCCGTTGC 
GGAGGTATTT TCCAGGAGGC CGTGAAGTGG GCGGCCTCTG CGACGCGCTC TCACATCCAG 
CAGTACATCA TCCATCTGGA GAACTCCTCG GACAGTCTCT CACAGCGTAG CACCCTGGAG 
CTCGCCACGG AGAGTGCCCT CCAGTGTGCA GGATTCAACA AAGGACTCAC CGCATCTGGT 
ACCGACAGTT TGACCAGTGA GTCAGCCAAC TTTGTATCAG TGATGAGCCT AAGGAGCAGG 
TTCGCTGGAG AGGTATCAGG CATGAAAGCA ATCTACACCG ACGCAGGGCC CGGGGGTTTG 
AGTCTATCTC ATGTACTGAA TAAACAGCTT GACAAGGCGT GTGATGCGAA GAAGATAGAT 
GCTTTCACTA ATTCTATGTT CAGAGTCTGT GCCCTCCTCA TCTCAAGTGA AGGTGTGGAT 
AGATACCTGT TGTATAGCCT GTGCAGGGCG CCTGTCAGGC TCTTCACAAC ACACGCTATG 
GAGAAGGCTG TTGCATGCTG GGAATGGCTG CTGGCTGCCA GATCGGACAT GGAACTGCAG 
TTAATGCGTG AGATGGCCGG AGCATGGCAG ATGAGCATGG ACAACAGACT TGGTCTGTTC 
TCTATTGATG TTGAAGATCC CAGTCCTCTG GCCGCCATCA GTGGCGATGA TGTCCCTGAC 
CCCAAACACC CAAACGCTAC ACCACATCAC ATCTGGACCC AGTTCTTACA CCAGCGCCTG 
GAGAGCATCA AGTACAGCAG CATCAACGTG GTGGAGATCT TCATCTCCAT CCTGCATCGC 
TCCCTACCCA TGCAGGTCGG TCAGACAAAG GGCGCCCTCT CCAGGCACAT CGCTGCCATG 
GGCCCCCGTT TCCGCCTGCT CTGTATGGGA CTGTCCCTCC TGCAGGGGGA TATCCTGCCC 
AGCAGTACGG GCAAGAACGT CTTGAGAGAA AGGATCTACT CGGCTGCGTT CGACTTCTTC 
AGTGTTGCTC CAGTGTGCCC AACCCAGTCT GTGAGTGAGC TGAGGGAAGA CATCAGTATT 
CTCATTAAGT TCTTCCATGC CATGCTGGCA GAGAAGAAAT ACCTCAGAGG CACCACAGCT 
GTGGTTCAAG AGTCCGAGGA TGTACACACG CTAGGCATCA TCCCAGCCGA CCTGCGAGCC 
AGTAACGAGT ACCTCAACGC CAGGCCAACA GCAGGGCGCC CTCAGGGTTG GATGAACACC 
ATGCCGCTGT CCAGTAACAT GTCCATCGTG TCCAAGAGGT CATCGGTCAC CAGGAAGAGT 
GGAGAGGTCG GAGCAAAGTA TGCCAAGGAG TACATGAAGA GGAGGAATCT AATCCTCTCT 
CTGCTGGTGA ATGAGATAGA GAGACTGTGT TGCTGGTGTA ATCCCTTAGA CAACTCAGAA 
ATCAAGCTAC CAGGAGAGGA GCTGGTTGCT AACTGGAGGG TCCAGACCCC TACCAATGAA 
CGCACCTGGA GGGACTTTGC TCACCTCGCC TGGGAGGTGT CCCCTCATCT GGCTTGTCAG 
CTTCCCGTCA GGTTCAAGTC TTCTGAGAGC CTTGTCAAGG AGGTGACGCG TATGGTGCGA 
CTCAACCCCA TGGCTGTCTC TGACCTCCCA AGTGCCATTC ACTTCCTGGT CACAGAACAC 
AGTGTGGATG CAGATGTCGC AGAGTTGAGT CACATCCTAT GCTGGGCGCC CGTTCCACCT 
GTCACCGCCC TGTCATACTT CTCCCAGCAG TACCCCCCGC ACCCCCTGAC TGCCCAGTAT 
GCCACCAGAG TCCTGCAGGA CTACCCACCA GAGGCACTGC TCTTCTATGT CCCTCAGCTA 
GTACAGGCTG TCAGATACGA TACGTTTGGC TTTGTGACGG AGCTGATCCT GGTCCTGGCG 
AAAAAGTCCC AACTCCTGGC CCATCAGCTC ATCTGGAACA TGAAGACCAA CATCTTCAAA 
GACGAGGAAG CCAAGGAAAG AGACGGTGAG TTTGCTGATG CCCTGGAGGC CCTGATCGAA 
GGCATCATAG CCAGCTTGTC GGGACCAGCG AAAGACTTCT ATGAGAGGGA GTTTGACTTT 
TTTGGCAAAA TAACAGCCAT CTCCGGCGAG ATCAGACCAT ATCCCAAGGG TGATGAAAGG 
AAAGCAGCCT GTCTGAAAGC CCTAGCAACC ATTCAACTCC AACCTGGATG CTATCTCCCT 
TGCAACCCTG AAGCCGTCGT CATGGAAATC GACTACAAGT CTGGAACTCC CATGCAGAGT 
GCTGCCAAGG CCCCGTTCCT TGCCAGGTTC AAGGTGCAGA AGTGCGGAGT TCATGGGCTG 
GAGAGCCTTG GTATGAAGGG CCTCGATTCA GACGCTGTTT CTCGTTCCCT CAAAGCACAA 
ATATGGGAGG CTTGCATCTT TAAGGTTGGA GATGACGTGA GACAGGACAT GCTTGCGCTG 
CAGGTGATTG GTCTATTCAA GAACATCTTC CATCAGGTGG GACTCAACCT CTACCTGGTC 
CCGTACAAAG TGGTAGCAAC GTCACCAGGG TGTGGAGTGA TCCAGTGTGT CCCAGACTGC 
AAGTCTCGTG ACCAGCTTGG CCGACAGACA GACATCGGCA TGTATGAATA CTTCCTAACC 
AAGTATGGAG ATGAGACTAC ACCCGCATAC CAGAAGGCTA GACGTCATTT CATCCAAAGC 
ATGGCAGCCT ACAGTGTGGT AGGGCACCTC CTCCAAATCA AAGACAGACA TAATGGAAAT 
ATCATGCTGG ATACCAGAGG CAACATCATA CACATTGATT TTGGTTTCAT GTTTGAGAGT 
TCTCCTGGAG GCAACCTGGG CTGGGAACCA GACATCAAGC TCACTGATGA AATGCTCATG 
ATCATGGGAG GCAATATAAA CGCGGCTCCA TTCAAGAAGT TTGAAGAACT GTGTGTACAG 
GCCTACCTAG CAGTCAGACC ATACCAAGAA GCCATTGTGT CCCTTGTAGC CCTGATGCTT 
GATACAGGAC TACCTTGCTT CAAAGGAAAA ACACTCAACT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC590935 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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