LOC590127 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC590127
Entrez Gene ID	590127
Full Name	proteasome activator complex subunit 4
Synonyms	psme4
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_030999469.1	XP_030855329.1	proteasome activator complex subunit 4
XM_011675563.1	XP_011673865.1	proteasome activator complex subunit 4 isoform X2
XM_789744.4	XP_794837.3	proteasome activator complex subunit 4 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC590127 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC590127 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd07437	XM_789744.4 Latest version!	Strongylocentrotus purpuratus proteasome activator complex subunit 4 (LOC590127), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSd26524	XM_011675563.1 Latest version!	Strongylocentrotus purpuratus proteasome activator complex subunit 4 (LOC590127), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSd65375	XM_030999469.1 Latest version!	Strongylocentrotus purpuratus proteasome activator complex subunit 4 (LOC590127), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC590127 ( XM_789744.4 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_794837.3 Strongylocentrotus purpuratus proteasome activator complex subunit 4 (LOC590127), transcript variant X1, mRNA.

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CloneID	OSd07437
Clone ID Related Accession (Same CDS sequence)	XM_789744.4
Accession Version	XM_789744.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	proteasome activator complex subunit 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011989134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 24, 2015 this sequence version replaced XM_789744.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGC AAGACGGGAA AGATCCCCGC TACCAAAAAG AATTGATTTA CAACAAATTG 
CTACCGTACT CCTCAGAATT TGAGGAGGAA TCGGAGAGAC ATCTGACTGA AATCAAAACC 
AATTTATCGC TTGCTGTGCA GCTTCGTGAA CTTCGCCCTG GTGTGCTGTT TTGGACCGGG 
AAGTTTTCGG CGTATATACG TTATTATGGC CGAAAGTTCA GCAAGGAAGA GCATGTTGCC 
TTCATCAAGC TCTATCTGGA ACTTATCAAC ATTCCAGATT TGGAATTCAA GATCATGTCA 
GAGTTTGCCA GAATTCTGAA CATACTTCTC AAGAAGCGAA GTTTGCTGTC ACGAGACGAC 
CTGCTTATAC CCTGGCGACC CCTCTACAAG ATTGTTGAAA GGATCATGTA CTCACCATAT 
GAACCATTGG GCTTAGAATG GTATCCACAC ACCCTGGAAA ATCGTCTAAA GTCCCTCATG 
CGGATGTCCA GACCATATTT CTCAGTTGAC AGTACTAGAG AAATGCTAGA GGAATGGCGC 
CCTCTCTTGT GTCCGTTTGA TGAGACCATG GCCAAGGGTA TCCATTACAT GGAGTGCTTT 
CTCCCAACCC TGCTTCCACC AGAACATCAC CAACATGGCT TCAAGTTATG GCTGGATGAA 
ATGATCAGTA TATGGAACAA TTCGCAGAAC TCTCCGCACA TAGAATCAGC ACTAGTAGAC 
CTATTTTCTA GATTAGCTAC AGAGAATATA GGATACATCG ACTGGAATCC ACATATACCA 
AAGATATTCA CATGTTTCTT GAGAGGCCTT AGTCTACCTG TTGGTTCCAA GAAGCTTCAG 
GTAGCTCGGA ATGGGAAGGG TTTTGATGTT GGTCCTCTTG CAACCTGGAT TGTTTCAATG 
CTGGGTCACA CAACAACTTG TCAGGACCAC TTACATAAGT TCTTTGTAGC AACAGATTCA 
TACTTCCATC CATCCAATAC TGGAAAATAC TCTTTCAGCC TTCAAAGGAT GCTTGGTCAG 
CTATCCAGTG AATTTGTCAA GAGATTACAC AGAGAGAGGT ATAAGAAGCC CTCGTGGCAG 
CACCAGATCC CTGATGATTA CAAGCTGAAA GAGGATGACA TCACAGCGTT TGTTGAGAGC 
ATCAAGGCTC CGGTGTTCCT AGCCATGTAC AGTAAGATGG GTCACAGGGA TGCTGCCTCG 
ACCCTGCATA ACCTGGCCTT GCTGAGACCT GAGCTGGTCA TACCAACACT GCTGGAAAAG 
ACCTACAAGG CTTTAGATAC CCTGACAGAG CCCCACCAGC TGATAGCCAC AGTGAGCTGC 
GTGGTGTCGG TCTCCTGCAC CATGCTCAAG GGGGGCAAGT GGCTCCCCGA TGGCCCCACT 
CACCTACTAC CTCTCCTCCA TGCGATGCTA CCTGGTATCG ATCCCAACGA CTTTGCCAAA 
TGCATGGTCA CACTGCAAGC CATCTCTACC TTCACCGTCA TGGTACCCAT AGTAGACTGC 
TCTGAGGCTT CCCTGGTTAG AGACGACATC ACGGAACAGG AGAGGGAGCT CTGTTCAGCA 
ACAGCCACCT TTGAAGATTT TGTGCTTCTC TTCATGGACA GATGTTTCAA TCTGATTGAG 
GCATGTTCCA TCGAGCATAT ACGAAGCCAG GACAACGATC AAGAAAAGGT GAACCACCAA 
GAAGCCATGG TGGACGTAGG ATTGTCTTCA ACATTCAGTA CCATCTGCTC CCAGAGCTCA 
CCTCAGATAT ACAAGGCAGC AGTCAATCGT TTGTTCCGCT ATGTTTCGTC GTCTATCCTT 
GAGACTAAGG TGTCTGGTAA AACCATTGCC AACATGTGCA GGGCAGCCGC TAAGGTGGAT 
CCCAAGTACA CTCTGTCCAA GTTTGTGCCT CACTGTAGCA AGAAGATCCT TGATGCCTTC 
ACATCAGAAG AGGTCCAGTT GGAAGAAGAG TTGGACAAGG AGATTCTCTT CAACCTCCAA 
GTTCTCTCAG AGGTTATACG AGTTCCTAGC GAGGTTGTCC TCACCTATAA GGACACCCTG 
AAAAAGGTCC TGAAGGCTAC CCTTCATCTG ACCTCTAAGA AAGGTTACGA GCTGTCCTGC 
CAGCTACTCA AGCACTGGCT ACGAGCTATG TGCCTCATGT ATCCTAGGGA GTACTGCAGT 
ATAGCAGAGG GCTTTGACAG GCCACTCACA GAGTACTTGC CTATCAGGGA CTGGGCAACG 
TGTGGTGACA TTCACAACCT GGGTGTGAAG TGGCATATAC CCTCTGATGA CGGAGTAGCA 
TGTGTGAAGG AGCTCTTAGA TATCTATTAC AAGCCAGAGA TAGAGAAACT ACAGAACTAC 
ATCAAGGGGG AGTCTATAAG CAGAGAGAAG CTACTACAGA GCCTGACCAT CTTGTTCAAT 
GTACTGACAG GGGCAGGGGT TCTTATGCCT AAATGGAAGA GGCCCTCGAT ACCAGAGCTG 
AGTGAGAGTC AGGTTCACAT GCACACTCTA GATAGGACAC TAGGATCAAC GGACAAACAG 
AAGAGGTTTG AAGGCATCAG AGAAGAACTA GCTCAGGTGA TGCATGATCT CCTTAATCAT 
CTACTAGCAA CAGGGGAGGA TGACACCAAG TCTATTATAC TCATCATCAA TATCAACAAT 
GCGCTGATGT GGTACATGGG AGCAGAGAAG AAGGACTTTG ACAGTCGATG GAAAGGATTC 
CTCATCATCA AGAAAGCCAT GGAAGACAGG TTACATGGGA AGAAGAGGCA CCTGCGAGCC 
CTGTTGATAG ATCGGGTATA CCTCCAGCAT GAGATGAGGA TGCTGGTCCA GGCCAAGCAT 
GTTTACACTG ATCTTGATAA ACAGATTCAT AGTGATCTGT TCACACTGTC AACAAGCAGG 
TACAGCGAGG TGCGTAAGAA GGCCCAGATC ATGCTGTTCA AGATGATGGA TCACTTTGTA 
TCATCAGTGA GGCTAATCAT TCCTAAGGTC TTGGTCAACC TCAAGCCAGA TACAGACATC 
CCACATCACC AGTTCAAGGG AGCCCTCTAT GTACTAAGTG GGTACAGCAT GAACAGCACA 
TGGGCATGCC TGCCTTACTG GTCAATCCTG AAAGACGTAT GGTTAGGACT GGTCAATGCC 
GGCCACTCTG AGAAGCTGTC CATCATGAAG GTCTTAGCTC AGGTCATGAT GAAGGTCAAC 
AAGGTCTTTG ACACGTCGGC CATCACGGTC AAGGTTTCAG ATGGCGCCAG GAGCAAGGCC 
AATGATTTGG TACAGGGTTT GAACGTCAGC ATAACGCCTG AGCAAGAATC AGCATCAGTA 
CAGAGGTTAC AGGAACACAA TCAAAGCAAC ATGAGAGTGT ATAATGAGCT GGTAGCAGAG 
CTGGTGGATG TAATAGAGCA AGGTAAACTG CGATGGAAGT TTTCTCAAAT GGCGGCAAGC 
CTCCTCCGTC TTCAGCTGAG GGTAGACACC CCTCCACCAA TCCCTGTCGT ACGGCTCTTC 
ACATCCCTCC TTGTACATGA CACTCTTAAC CTAAGAAAGC TTGCCATTTC AGCCATGGGT 
GCAATAATGA AGCAACTGAA GCATCCCCAC AAGAAGGTGC CCCTGGACCC CTACGCCACA 
GCTGGGTGCC CCGCCCCTCC TGACGACGCC CTACCCAACC CCGGGGAGCG GCCGGACAAC 
AAGTGGACCC AGTACCAGTG TGACAGGTTG CCCACCACCC AGGAGAAATG GGACCAGTGT 
GTCTTTGTGG ACAAGACTCA CTGGGGGTAC TATAAATGGC CCAAGAACAT GCAGATCTAC 
GCCCCATTCA ATGAACAGCC CAAGCTAGAC CGTAGCAGAG AGGAGCTGTC AGAGACGGAA 
CAGATCATCT ATGATCACTT CAGCAATGCA GAGTTTGTAG AGAAACTAGT AGGCTACCTG 
TCCTTAGAAG ATAAGAAAGG AAGAGACAAG TTTGACAACA AGAAATACAC AGTCTTCAAG 
GGACTGTTCC GTAACTTTGG GGACACTTTC CTGCCAGTCT TCCAGCCCCA CATAGAGAGG 
TTGTCCAAGG AGGCACTGGA GAGTAGCCAG CGGAGTATGA TGGAGATGAT GGCTGGTGTT 
ATACGAGGCA GCAAGCACTG GGGCTTTCAA AAGACCAAGA ATCTGTGGGA GACTGTGCTG 
CTACCTTCTA TGAAGACATC ATTGTCCTTG GTAACAGCAG AGACCATCAT AGATTGGGGG 
ATGTGCATGG CTACGTCATG TGAGAGTAGA GACCCTCGAA AGCTTCATTG GCTGTTTGAA 
TGGCTCACCC AACAACCAAT CAGTGAAGAT GAGGAGAGGG GGTCTTTCCT GGATTCAAGT 
CGTCTGTTCA TCCTACAAGG TGGATTGTTT CAGCAGGAGT GGAGAGTACC AGGTCTTCAT 
CACAGACTGT TAGGCTACAT ACGACAGCAT CTTACCCACA GCTACAAGAA TGTCAGGGAC 
AAGATCGGCA GCTTCCTGGT CATGATCTTC ATGTACGACA ATGCCTGGTC CCGGGAGAAC 
CAGACATCCT CCCCTCACCG CCAAGAGTTC TTAGAATCCA TCCTACCCCA GATGGCCCCC 
CTGGCTAACA TAGACGTCTG GGGAGCTATC CAGGTCTCTG ATGAAGAGAA GGCAGAGGAT 
GATGAGAGGA AGAAGGTCAT TAGGTTATGT AAAACAGTCA TGAAGTGGAT TGTAGGTAGC 
CTAGGGAACC TTGTATCTCC TGCACCTCAA GAATACTTCC AGTACCTTCC TCTGTTTGCA 
CCTCTTGAGA GCTGTGAGTC TGATGAAGAG CTACGGTATG AAGCAGAGAT GACCCTAGCC 
TGTCTAGCCC AGACTTTACT TCAACCAGGG ATGATACCCC TTGTACTCTC CTCTCTACAG 
CAGATTGGTC AGGGCCAATC CTGGAGAGCC AAGTGTAGTA TGTTAGAGTA CCTTCAGGTG 
ATGGTGTTCT ACAACCTCTT CACCATCAAC AGTCTACCCA AGCATATAGA AACCATTGGT 
CAACTCGTAC TGGACAGGCT CAAGGACGAT CGCCTTGAGG TGAGAGAGGT AGCAGGTGCA 
ACACTGAGCG GTCTGATTCA CTGTGGCTTC TTACCAATGG AGAATGGGAT GCAGAGCCAC 
TTTGAGAACC TGTGTAAGAC AAGACTATCG ACCAAGAAAG TAGTCAGTAT GGCTGCACCC 
GTTGAAGCCA TAGTCCAGAG GCATGCTGGT ATCCTGGGTC TGAGTGCATG TGTCCAAGCC 
TACCCCTATG ATGTCCCTGA ATGGATGCCA AGGATACTGA TGGACCTCAC AGATCATCTA 
CACGATCCTC AACCTATCGA GAAAACAGTG CGAAAAACCC TGTCTGACTT CCGCAGAACG 
CATCACGACA ACTGGCACGA GCACAAGCAG AGCTTCACAG ACGACCAGCT TGTAGTACTT 
ACTGACCTTC TAGTCTCGCC CAACTACTAC GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_794837.3
CDS	131..5566
Translation	MDEQDGKDPRYQKELIYNKLLPYSSEFEEESERHLTEIKTNLSLAVQLRELRPGVLFWTGKFSAYIRYYGRKFSKEEHVAFIKLYLELINIPDLEFKIMSEFARILNILLKKRSLLSRDDLLIPWRPLYKIVERIMYSPYEPLGLEWYPHTLENRLKSLMRMSRPYFSVDSTREMLEEWRPLLCPFDETMAKGIHYMECFLPTLLPPEHHQHGFKLWLDEMISIWNNSQNSPHIESALVDLFSRLATENIGYIDWNPHIPKIFTCFLRGLSLPVGSKKLQVARNGKGFDVGPLATWIVSMLGHTTTCQDHLHKFFVATDSYFHPSNTGKYSFSLQRMLGQLSSEFVKRLHRERYKKPSWQHQIPDDYKLKEDDITAFVESIKAPVFLAMYSKMGHRDAASTLHNLALLRPELVIPTLLEKTYKALDTLTEPHQLIATVSCVVSVSCTMLKGGKWLPDGPTHLLPLLHAMLPGIDPNDFAKCMVTLQAISTFTVMVPIVDCSEASLVRDDITEQERELCSATATFEDFVLLFMDRCFNLIEACSIEHIRSQDNDQEKVNHQEAMVDVGLSSTFSTICSQSSPQIYKAAVNRLFRYVSSSILETKVSGKTIANMCRAAAKVDPKYTLSKFVPHCSKKILDAFTSEEVQLEEELDKEILFNLQVLSEVIRVPSEVVLTYKDTLKKVLKATLHLTSKKGYELSCQLLKHWLRAMCLMYPREYCSIAEGFDRPLTEYLPIRDWATCGDIHNLGVKWHIPSDDGVACVKELLDIYYKPEIEKLQNYIKGESISREKLLQSLTILFNVLTGAGVLMPKWKRPSIPELSESQVHMHTLDRTLGSTDKQKRFEGIREELAQVMHDLLNHLLATGEDDTKSIILIININNALMWYMGAEKKDFDSRWKGFLIIKKAMEDRLHGKKRHLRALLIDRVYLQHEMRMLVQAKHVYTDLDKQIHSDLFTLSTSRYSEVRKKAQIMLFKMMDHFVSSVRLIIPKVLVNLKPDTDIPHHQFKGALYVLSGYSMNSTWACLPYWSILKDVWLGLVNAGHSEKLSIMKVLAQVMMKVNKVFDTSAITVKVSDGARSKANDLVQGLNVSITPEQESASVQRLQEHNQSNMRVYNELVAELVDVIEQGKLRWKFSQMAASLLRLQLRVDTPPPIPVVRLFTSLLVHDTLNLRKLAISAMGAIMKQLKHPHKKVPLDPYATAGCPAPPDDALPNPGERPDNKWTQYQCDRLPTTQEKWDQCVFVDKTHWGYYKWPKNMQIYAPFNEQPKLDRSREELSETEQIIYDHFSNAEFVEKLVGYLSLEDKKGRDKFDNKKYTVFKGLFRNFGDTFLPVFQPHIERLSKEALESSQRSMMEMMAGVIRGSKHWGFQKTKNLWETVLLPSMKTSLSLVTAETIIDWGMCMATSCESRDPRKLHWLFEWLTQQPISEDEERGSFLDSSRLFILQGGLFQQEWRVPGLHHRLLGYIRQHLTHSYKNVRDKIGSFLVMIFMYDNAWSRENQTSSPHRQEFLESILPQMAPLANIDVWGAIQVSDEEKAEDDERKKVIRLCKTVMKWIVGSLGNLVSPAPQEYFQYLPLFAPLESCESDEELRYEAEMTLACLAQTLLQPGMIPLVLSSLQQIGQGQSWRAKCSMLEYLQVMVFYNLFTINSLPKHIETIGQLVLDRLKDDRLEVREVAGATLSGLIHCGFLPMENGMQSHFENLCKTRLSTKKVVSMAAPVEAIVQRHAGILGLSACVQAYPYDVPEWMPRILMDLTDHLHDPQPIEKTVRKTLSDFRRTHHDNWHEHKQSFTDDQLVVLTDLLVSPNYYA

Target ORF information:

RefSeq Version	XM_789744.4
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus proteasome activator complex subunit 4 (LOC590127), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_789744.4

ORF Insert Sequence:

ATGGATGAGC AAGACGGGAA AGATCCCCGC TACCAAAAAG AATTGATTTA CAACAAATTG 
CTACCGTACT CCTCAGAATT TGAGGAGGAA TCGGAGAGAC ATCTGACTGA AATCAAAACC 
AATTTATCGC TTGCTGTGCA GCTTCGTGAA CTTCGCCCTG GTGTGCTGTT TTGGACCGGG 
AAGTTTTCGG CGTATATACG TTATTATGGC CGAAAGTTCA GCAAGGAAGA GCATGTTGCC 
TTCATCAAGC TCTATCTGGA ACTTATCAAC ATTCCAGATT TGGAATTCAA GATCATGTCA 
GAGTTTGCCA GAATTCTGAA CATACTTCTC AAGAAGCGAA GTTTGCTGTC ACGAGACGAC 
CTGCTTATAC CCTGGCGACC CCTCTACAAG ATTGTTGAAA GGATCATGTA CTCACCATAT 
GAACCATTGG GCTTAGAATG GTATCCACAC ACCCTGGAAA ATCGTCTAAA GTCCCTCATG 
CGGATGTCCA GACCATATTT CTCAGTTGAC AGTACTAGAG AAATGCTAGA GGAATGGCGC 
CCTCTCTTGT GTCCGTTTGA TGAGACCATG GCCAAGGGTA TCCATTACAT GGAGTGCTTT 
CTCCCAACCC TGCTTCCACC AGAACATCAC CAACATGGCT TCAAGTTATG GCTGGATGAA 
ATGATCAGTA TATGGAACAA TTCGCAGAAC TCTCCGCACA TAGAATCAGC ACTAGTAGAC 
CTATTTTCTA GATTAGCTAC AGAGAATATA GGATACATCG ACTGGAATCC ACATATACCA 
AAGATATTCA CATGTTTCTT GAGAGGCCTT AGTCTACCTG TTGGTTCCAA GAAGCTTCAG 
GTAGCTCGGA ATGGGAAGGG TTTTGATGTT GGTCCTCTTG CAACCTGGAT TGTTTCAATG 
CTGGGTCACA CAACAACTTG TCAGGACCAC TTACATAAGT TCTTTGTAGC AACAGATTCA 
TACTTCCATC CATCCAATAC TGGAAAATAC TCTTTCAGCC TTCAAAGGAT GCTTGGTCAG 
CTATCCAGTG AATTTGTCAA GAGATTACAC AGAGAGAGGT ATAAGAAGCC CTCGTGGCAG 
CACCAGATCC CTGATGATTA CAAGCTGAAA GAGGATGACA TCACAGCGTT TGTTGAGAGC 
ATCAAGGCTC CGGTGTTCCT AGCCATGTAC AGTAAGATGG GTCACAGGGA TGCTGCCTCG 
ACCCTGCATA ACCTGGCCTT GCTGAGACCT GAGCTGGTCA TACCAACACT GCTGGAAAAG 
ACCTACAAGG CTTTAGATAC CCTGACAGAG CCCCACCAGC TGATAGCCAC AGTGAGCTGC 
GTGGTGTCGG TCTCCTGCAC CATGCTCAAG GGGGGCAAGT GGCTCCCCGA TGGCCCCACT 
CACCTACTAC CTCTCCTCCA TGCGATGCTA CCTGGTATCG ATCCCAACGA CTTTGCCAAA 
TGCATGGTCA CACTGCAAGC CATCTCTACC TTCACCGTCA TGGTACCCAT AGTAGACTGC 
TCTGAGGCTT CCCTGGTTAG AGACGACATC ACGGAACAGG AGAGGGAGCT CTGTTCAGCA 
ACAGCCACCT TTGAAGATTT TGTGCTTCTC TTCATGGACA GATGTTTCAA TCTGATTGAG 
GCATGTTCCA TCGAGCATAT ACGAAGCCAG GACAACGATC AAGAAAAGGT GAACCACCAA 
GAAGCCATGG TGGACGTAGG ATTGTCTTCA ACATTCAGTA CCATCTGCTC CCAGAGCTCA 
CCTCAGATAT ACAAGGCAGC AGTCAATCGT TTGTTCCGCT ATGTTTCGTC GTCTATCCTT 
GAGACTAAGG TGTCTGGTAA AACCATTGCC AACATGTGCA GGGCAGCCGC TAAGGTGGAT 
CCCAAGTACA CTCTGTCCAA GTTTGTGCCT CACTGTAGCA AGAAGATCCT TGATGCCTTC 
ACATCAGAAG AGGTCCAGTT GGAAGAAGAG TTGGACAAGG AGATTCTCTT CAACCTCCAA 
GTTCTCTCAG AGGTTATACG AGTTCCTAGC GAGGTTGTCC TCACCTATAA GGACACCCTG 
AAAAAGGTCC TGAAGGCTAC CCTTCATCTG ACCTCTAAGA AAGGTTACGA GCTGTCCTGC 
CAGCTACTCA AGCACTGGCT ACGAGCTATG TGCCTCATGT ATCCTAGGGA GTACTGCAGT 
ATAGCAGAGG GCTTTGACAG GCCACTCACA GAGTACTTGC CTATCAGGGA CTGGGCAACG 
TGTGGTGACA TTCACAACCT GGGTGTGAAG TGGCATATAC CCTCTGATGA CGGAGTAGCA 
TGTGTGAAGG AGCTCTTAGA TATCTATTAC AAGCCAGAGA TAGAGAAACT ACAGAACTAC 
ATCAAGGGGG AGTCTATAAG CAGAGAGAAG CTACTACAGA GCCTGACCAT CTTGTTCAAT 
GTACTGACAG GGGCAGGGGT TCTTATGCCT AAATGGAAGA GGCCCTCGAT ACCAGAGCTG 
AGTGAGAGTC AGGTTCACAT GCACACTCTA GATAGGACAC TAGGATCAAC GGACAAACAG 
AAGAGGTTTG AAGGCATCAG AGAAGAACTA GCTCAGGTGA TGCATGATCT CCTTAATCAT 
CTACTAGCAA CAGGGGAGGA TGACACCAAG TCTATTATAC TCATCATCAA TATCAACAAT 
GCGCTGATGT GGTACATGGG AGCAGAGAAG AAGGACTTTG ACAGTCGATG GAAAGGATTC 
CTCATCATCA AGAAAGCCAT GGAAGACAGG TTACATGGGA AGAAGAGGCA CCTGCGAGCC 
CTGTTGATAG ATCGGGTATA CCTCCAGCAT GAGATGAGGA TGCTGGTCCA GGCCAAGCAT 
GTTTACACTG ATCTTGATAA ACAGATTCAT AGTGATCTGT TCACACTGTC AACAAGCAGG 
TACAGCGAGG TGCGTAAGAA GGCCCAGATC ATGCTGTTCA AGATGATGGA TCACTTTGTA 
TCATCAGTGA GGCTAATCAT TCCTAAGGTC TTGGTCAACC TCAAGCCAGA TACAGACATC 
CCACATCACC AGTTCAAGGG AGCCCTCTAT GTACTAAGTG GGTACAGCAT GAACAGCACA 
TGGGCATGCC TGCCTTACTG GTCAATCCTG AAAGACGTAT GGTTAGGACT GGTCAATGCC 
GGCCACTCTG AGAAGCTGTC CATCATGAAG GTCTTAGCTC AGGTCATGAT GAAGGTCAAC 
AAGGTCTTTG ACACGTCGGC CATCACGGTC AAGGTTTCAG ATGGCGCCAG GAGCAAGGCC 
AATGATTTGG TACAGGGTTT GAACGTCAGC ATAACGCCTG AGCAAGAATC AGCATCAGTA 
CAGAGGTTAC AGGAACACAA TCAAAGCAAC ATGAGAGTGT ATAATGAGCT GGTAGCAGAG 
CTGGTGGATG TAATAGAGCA AGGTAAACTG CGATGGAAGT TTTCTCAAAT GGCGGCAAGC 
CTCCTCCGTC TTCAGCTGAG GGTAGACACC CCTCCACCAA TCCCTGTCGT ACGGCTCTTC 
ACATCCCTCC TTGTACATGA CACTCTTAAC CTAAGAAAGC TTGCCATTTC AGCCATGGGT 
GCAATAATGA AGCAACTGAA GCATCCCCAC AAGAAGGTGC CCCTGGACCC CTACGCCACA 
GCTGGGTGCC CCGCCCCTCC TGACGACGCC CTACCCAACC CCGGGGAGCG GCCGGACAAC 
AAGTGGACCC AGTACCAGTG TGACAGGTTG CCCACCACCC AGGAGAAATG GGACCAGTGT 
GTCTTTGTGG ACAAGACTCA CTGGGGGTAC TATAAATGGC CCAAGAACAT GCAGATCTAC 
GCCCCATTCA ATGAACAGCC CAAGCTAGAC CGTAGCAGAG AGGAGCTGTC AGAGACGGAA 
CAGATCATCT ATGATCACTT CAGCAATGCA GAGTTTGTAG AGAAACTAGT AGGCTACCTG 
TCCTTAGAAG ATAAGAAAGG AAGAGACAAG TTTGACAACA AGAAATACAC AGTCTTCAAG 
GGACTGTTCC GTAACTTTGG GGACACTTTC CTGCCAGTCT TCCAGCCCCA CATAGAGAGG 
TTGTCCAAGG AGGCACTGGA GAGTAGCCAG CGGAGTATGA TGGAGATGAT GGCTGGTGTT 
ATACGAGGCA GCAAGCACTG GGGCTTTCAA AAGACCAAGA ATCTGTGGGA GACTGTGCTG 
CTACCTTCTA TGAAGACATC ATTGTCCTTG GTAACAGCAG AGACCATCAT AGATTGGGGG 
ATGTGCATGG CTACGTCATG TGAGAGTAGA GACCCTCGAA AGCTTCATTG GCTGTTTGAA 
TGGCTCACCC AACAACCAAT CAGTGAAGAT GAGGAGAGGG GGTCTTTCCT GGATTCAAGT 
CGTCTGTTCA TCCTACAAGG TGGATTGTTT CAGCAGGAGT GGAGAGTACC AGGTCTTCAT 
CACAGACTGT TAGGCTACAT ACGACAGCAT CTTACCCACA GCTACAAGAA TGTCAGGGAC 
AAGATCGGCA GCTTCCTGGT CATGATCTTC ATGTACGACA ATGCCTGGTC CCGGGAGAAC 
CAGACATCCT CCCCTCACCG CCAAGAGTTC TTAGAATCCA TCCTACCCCA GATGGCCCCC 
CTGGCTAACA TAGACGTCTG GGGAGCTATC CAGGTCTCTG ATGAAGAGAA GGCAGAGGAT 
GATGAGAGGA AGAAGGTCAT TAGGTTATGT AAAACAGTCA TGAAGTGGAT TGTAGGTAGC 
CTAGGGAACC TTGTATCTCC TGCACCTCAA GAATACTTCC AGTACCTTCC TCTGTTTGCA 
CCTCTTGAGA GCTGTGAGTC TGATGAAGAG CTACGGTATG AAGCAGAGAT GACCCTAGCC 
TGTCTAGCCC AGACTTTACT TCAACCAGGG ATGATACCCC TTGTACTCTC CTCTCTACAG 
CAGATTGGTC AGGGCCAATC CTGGAGAGCC AAGTGTAGTA TGTTAGAGTA CCTTCAGGTG 
ATGGTGTTCT ACAACCTCTT CACCATCAAC AGTCTACCCA AGCATATAGA AACCATTGGT 
CAACTCGTAC TGGACAGGCT CAAGGACGAT CGCCTTGAGG TGAGAGAGGT AGCAGGTGCA 
ACACTGAGCG GTCTGATTCA CTGTGGCTTC TTACCAATGG AGAATGGGAT GCAGAGCCAC 
TTTGAGAACC TGTGTAAGAC AAGACTATCG ACCAAGAAAG TAGTCAGTAT GGCTGCACCC 
GTTGAAGCCA TAGTCCAGAG GCATGCTGGT ATCCTGGGTC TGAGTGCATG TGTCCAAGCC 
TACCCCTATG ATGTCCCTGA ATGGATGCCA AGGATACTGA TGGACCTCAC AGATCATCTA 
CACGATCCTC AACCTATCGA GAAAACAGTG CGAAAAACCC TGTCTGACTT CCGCAGAACG 
CATCACGACA ACTGGCACGA GCACAAGCAG AGCTTCACAG ACGACCAGCT TGTAGTACTT 
ACTGACCTTC TAGTCTCGCC CAACTACTAC GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC590127 ( XM_011675563.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011673865.1 Strongylocentrotus purpuratus proteasome activator complex subunit 4 (LOC590127), transcript variant X2, mRNA.

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CloneID	OSd26524
Clone ID Related Accession (Same CDS sequence)	XM_011675563.1
Accession Version	XM_011675563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	proteasome activator complex subunit 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011989134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGC AAGACGGGAA AGATCCCCGC TACCAAAAAG AATTGATTTA CAACAAATTG 
CTACCGTACT CCTCAGAATT TGAGGAGGAA TCGGAGAGAC ATCTGACTGA AATCAAAACC 
AATTTATCGC TTGCTGTGCA GCTTCGTGAA CTTCGCCCTG GTGTGCTTTT TTGGACCGGG 
AAGTTTTCGG CGTATATACG TTATTATGGC CGAAAGTTCA GCAAGGAAGA GCATGTTGCC 
TTCATCAAGC TCTATCTGGA ACTTATCAAC ATTCCAGATT TGGAATTCAA GATCATGTCA 
GAGTTTGCCA GAATTCTGAA CATACTTCTC AAGAAGCGAA GTTTGCTGTC ACGAGACGAC 
CTGCTTATAC CCTGGCGACC CCTCTACAAG ATTGTTGAAA GGATCATGTA CTCACCATAT 
GAACCATTGG GCTTAGAATG GTATCCACAC ACCCTGGAAA ATCGTCTAAA GTCCCTCATG 
CGGATGTCCA GACCATATTT CTCAGTTGAC AGTACTAGAG AAATGCTAGA GGAATGGCGC 
CCTCTCTTGT GTCCGTTTGA TGAGACCATG GCCAAGGGTA TCCATTACAT GGAGTGCTTT 
CTCCCAACCC TGCTTCCACC AGAACATCAC CAACATGGCT TCAAGTTATG GCTGGATGAA 
ATGATCAGTA TATGGAACAA TTCGCAGAAC TCTCCGCACA TAGAATCAGC ACTAGTAGAC 
CTATTTTCTA GATTAGCTAC AGAGAATATA GGATACATCG ACTGGAATCC ACATATACCA 
AAGATATTCA CATGTTTCTT GAGAGGCCTT AGTCTACCTG TTGGTTCCAA GAAGCTTCAG 
GTAGCTCGGA ATGGGAAGGG TTTTGATGTT GGTCCTCTTG CAACCTGGAT TGTTTCAATG 
CTGGGTCACA CAACAACTTG TCAGGACCAC TTACATAAGT TCTTTGTAGC AACAGATTCA 
TACTTCCATC CATCCAATAC TGGAAAATAC TCTTTCAGCC TTCAAAGGAT GCTTGGTCAG 
CTATCCAGTG AATTTGTCAA GAGATTACAC AGAGAGAGGT ATAAGAAGCC CTCGTGGCAG 
CACCAGATCC CTGATGATTA CAAGCTGAAA GAGGATGACA TCACAGCGTT TGTTGAGAGC 
ATCAAGGCTC CGGTGTTCCT AGCCATGTAC AGTAAGATGG GTCACAGGGA TGCTGCCTCG 
ACCCTGCATA ACCTGGCCTT GCTGAGACCT GAGCTGGTCA TACCAACACT GCTGGAAAAG 
ACCTACAAGG CTTTAGATAC CCTGACAGAG CCCCACCAGC TGATAGCCAC AGTGAGCTGC 
GTGGTGTCGG TCTCCTGCAC CATGCTCAAG GGGGGCAAGT GGCTCCCCGA TGGCCCCACT 
CACCTACTAC CTCTCCTCCA TGCGATGCTA CCTGGTATCG ATCCCAACGA CTTTGCCAAA 
TGCATGGTCA CACTGCAAGC CATCTCTACC TTCACCGTCA TGGTACCCAT AGTAGACTGC 
TCTGAGGCTT CCCTGGTTAG AGACGACATC ACGGAACAGG AGAGGGAGCT CTGTTCAGCA 
ACAGCCACCT TTGAAGATTT TGTGCTTCTC TTCATGGACA GATGTTTCAA TCTGATTGAG 
GCATGTTCCA TCGAGCATAT ACGAAGCCAG GACAACGATC AAGAAAAGGT GAACCACCAA 
GAAGCCATGG TGGACGTAGG ATTGTCTTCA ACATTCAGTA CCATCTGCTC CCAGAGCTCA 
CCTCAGATAT ACAAGGCAGC AGTCAATCGT TTGTTCCGCT ATGTTTCGTC GTCTATCCTT 
GAGACTAAGG TGTCTGGTAA AACCATTGCC AACATGTGCA GGGCAGCCGC TAAGGTGGAT 
CCCAAGTACA CTCTGTCCAA GTTTGTGCCT CACTGTAGCA AGAAGATCCT TGATGCCTTC 
ACATCAGAAG AGGTCCAGTT GGAAGAAGAG TTGGACAAGG AGATTCTCTT CAACCTCCAA 
GTTCTCTCAG AGGTTATACG AGTTCCTAGC GAGGTTGTCC TCACCTATAA GGACACCCTG 
AAAAAGGTCC TGAAGGCTAC CCTTCATCTG ACCTCTAAGA AAGGTTACGA GCTGTCCTGC 
CAGCTACTCA AGCACTGGCT ACGAGCTATG TGCCTCATGT ATCCTAGGGA GTACTGCAGT 
ATAGCAGAGG GCTTTGACAG GCCACTCACA GAGTACTTGC CTATCAGGGA CTGGGCAACG 
TGTGGTGACA TTCACAACCT GGGTGTGAAG TGGCATATAC CCTCTGATGA CGGAGTAGCA 
TGTGTGAAGG AGCTCTTAGA TATCTATTAC AAGCCAGAGA TAGAGAAACT ACAGAACTAC 
ATCAAGGGGG AGTCTATAAG CAGAGAGAAG CTACTACAGA GCCTGACCAT CTTGTTCAAT 
GTACTGACAG GGGCAGGGGT TCTTATGCCT AAATGGAAGA GGCCCTCGAT ACCAGAGCTG 
AGTGAGAGTC AGGTTCACAT GCACACTCTA GATAGGACAC TAGGATCAAC GGACAAACAG 
AAGAGGTTTG AAGGCATCAG AGAAGAACTA GCTCAGGTGA TGCATGATCT CCTTAATCAT 
CTACTAGCAA CAGGGGAGGA TGACACCAAG TCTATTATAC TCATCATCAA TATCAACAAT 
GCGCTGATGT GGTACATGGG AGCAGAGAAG AAGGACTTTG ACAGTCGATG GAAAGGATTC 
CTCATCATCA AGAAAGCCAT GGAAGACAGG TTACATGGGA AGAAGAGGCA CCTGCGAGCC 
CTGTTGATAG ATCGGGTATA CCTCCAGCAT GAGATGAGGA TGCTGGTCCA GGCCAAGCAT 
GTTTACACTG ATCTTGATAA ACAGATTCAT AGTGATCTGT TCACACTGTC AACAAGCAGG 
TACAGCGAGG TGCGTAAGAA GGCCCAGATC ATGCTGTTCA AGATGATGGA TCACTTTGTA 
TCATCAGTGA GGCTAATCAT TCCTAAGGTC TTGGTCAACC TCAAGCCAGA TACAGACATC 
CCACATCACC AGTTCAAGGG AGCCCTCTAT GTACTAAGTG GGTACAGCAT GAACAGCACA 
TGGGCATGCC TGCCTTACTG GTCAATCCTG AAAGACGTAT GGTTAGGACT GGTCAATGCC 
GGCCACTCTG AGAAGCTGTC CATCATGAAG GTCTTAGCTC AGGTCATGAT GAAGGTCAAC 
AAGGTCTTTG ACACGTCGGC CATCACGGTC AAGGTTTCAG ATGGCGCCAG GAGCAAGGCC 
AATGATTTGG TACAGGGTTT GAACGTCAGC ATAACGCCTG AGCAAGAATC AGCATCAGTA 
CAGAGGTTAC AGGAACACAA TCAAAGCAAC ATGAGAGTGT ATAATGAGCT GGTAGCAGAG 
CTGGTGGATG TAATAGAGCA AGGTAAACTG CGATGGAAGT TTTCTCAAAT GGCGGCAAGC 
CTCCTCCGTC TTCAGCTGAG GGTAGACACC CCTCCACCAA TCCCTGTCGT ACGGCTCTTC 
ACATCCCTCC TTGTACATGA CACTCTTAAC CTAAGAAAGC TTGCCATTTC AGCCATGGGT 
GCAATAATGA AGCAACTGAA GCATCCCCAC AAGAAGGTGC CCCTGGACCC CTACGCCACA 
GCTGGGTGCC CCGCCCCTCC TGACGACGCC CTACCCAACC CCGGGGAGCG GCCGGACAAC 
AAGTGGACCC AGTACCAGTG TGACAGGTTG CCCACCACCC AGGAGAAATG GGACCAGTGT 
GTCTTTGTGG ACAAGACTCA CTGGGGGTAC TATAAATGGC CCAAGAACAT GCAGATCTAC 
GCCCCATTCA ATGAACAGCC CAAGCTAGAC CGTAGCAGAG AGGAGCTGTC AGAGACGGAA 
CAGATCATCT ATGATCACTT CAGCAATGCA GAGTTTGTAG AGAAACTAGT AGGCTACCTG 
TCCTTAGAAG ATAAGAAAGG AAGAGACAAG TTTGACAACA AGAAATACAC AGTCTTCAAG 
GGACTGTTCC GTAACTTTGG GGACACTTTC CTGCCAGTCT TCCAGCCCCA CATAGAGAGG 
TTGTCCAAGG AGGCACTGGA GAGTAGCCAG CGGAGTATGA TGGAGATGAT GGCTGGTGTT 
ATACGAGGCA GCAAGCACTG GGGCTTTCAA AAGACCAAGA ATCTGTGGGA GACTGTGCTG 
CTACCTTCTA TGAAGACATC ATTGTCCTTG GTAACAGCAG AGACCATCAT AGATTGGGGG 
ATGTGCATGG CTACGTCATG TGAGAGTAGA GACCCTCGAA AGCTTCATTG GCTGTTTGAA 
TGGCTCACCC AACAACCAAT CAGTGAAGAT GAGGAGAGGG GGTCTTTCCT GGATTCAAGT 
CGTCTGTTCA TCCTACAAGG TGGATTGTTT CAGCAGGAGT GGAGAGTACC AGGTCTTCAT 
CACAGACTGT TAGGCTACAT ACGACAGCAT CTTACCCACA GCTACAAGAA TGTCAGGGAC 
AAGATCGGCA GCTTCCTGGT CATGATCTTC ATGTACGACA ATGCCTGGTC CCGGGAGAAC 
CAGACATCCT CCCCTCACCG CCAAGAGTTC TTAGAATCCA TCCTACCCCA GATGGCCCCC 
CTGGCTAACA TAGACGTCTG GGGAGCTATC CAGGTCTCTG ATGAAGAGAA GGCAGAGGAT 
GATGAGAGGA AGAAGGTCAT TAGGTTATGT AAAACAGTCA TGAAGTGGAT TGTAGGTAGC 
CTAGGGAACC TTGTATCTCC TGCACCTCAA GAATACTTCC AGTACCTTCC TCTGTTTGCA 
CCTCTTGAGA GCTGTGAGTC TGATGAAGAG CTACGGTATG AAGCAGAGAT GACCCTAGCC 
TGTCTAGCCC AGACTTTACT TCAACCAGGG ATGATACCCC TTGTACTCTC CTCTCTACAG 
CAGATTGGTC AGGGCCAATC CTGGAGAGCC AAGTGTAGTA TGTTAGAGTA CCTTCAGGTG 
ATGGTGTTCT ACAACCTCTT CACCATCAAC AGTCTACCCA AGCATATAGA AACCATTGGT 
CAACTCGTAC TGGACAGGCT CAAGGACGAT CGCCTTGAGG TGAGAGAGGT AGCAGGTGCA 
ACACTGAGCG GTCTGATTCA CTGTGGCTTC TTACCAATGG AGAATGGGAT GCAGAGCCAC 
TTTGAGAACC TGTGTAAGAC AAGACTATCG ACCAAGAAAG TAGTCAGTAT GGCTGCACCC 
GTTGAAGCCA TAGTCCAGAG GCATGCTGGT ATCCTGGGTC TGAGTGCATG TGTCCAAGCC 
TACCCCTATG ATGTCCCTGA ATGGATGCCA AGGATACTGA TGGACCTCAC AGATCATCTA 
CACGATCCTC AACCTATCGA GAAAACAGTG CGAAAAACCC TGTCTGACTT CCGCAGAACG 
CATCACGACA ACTGGCACGA GCACAAGCAG AGCTTCACAG ACGACCAGCT TGTAGTACTT 
ACTGACCTTC TAGTCTCGCC CAACTACTAC GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011673865.1
CDS	131..5566
Translation	MDEQDGKDPRYQKELIYNKLLPYSSEFEEESERHLTEIKTNLSLAVQLRELRPGVLFWTGKFSAYIRYYGRKFSKEEHVAFIKLYLELINIPDLEFKIMSEFARILNILLKKRSLLSRDDLLIPWRPLYKIVERIMYSPYEPLGLEWYPHTLENRLKSLMRMSRPYFSVDSTREMLEEWRPLLCPFDETMAKGIHYMECFLPTLLPPEHHQHGFKLWLDEMISIWNNSQNSPHIESALVDLFSRLATENIGYIDWNPHIPKIFTCFLRGLSLPVGSKKLQVARNGKGFDVGPLATWIVSMLGHTTTCQDHLHKFFVATDSYFHPSNTGKYSFSLQRMLGQLSSEFVKRLHRERYKKPSWQHQIPDDYKLKEDDITAFVESIKAPVFLAMYSKMGHRDAASTLHNLALLRPELVIPTLLEKTYKALDTLTEPHQLIATVSCVVSVSCTMLKGGKWLPDGPTHLLPLLHAMLPGIDPNDFAKCMVTLQAISTFTVMVPIVDCSEASLVRDDITEQERELCSATATFEDFVLLFMDRCFNLIEACSIEHIRSQDNDQEKVNHQEAMVDVGLSSTFSTICSQSSPQIYKAAVNRLFRYVSSSILETKVSGKTIANMCRAAAKVDPKYTLSKFVPHCSKKILDAFTSEEVQLEEELDKEILFNLQVLSEVIRVPSEVVLTYKDTLKKVLKATLHLTSKKGYELSCQLLKHWLRAMCLMYPREYCSIAEGFDRPLTEYLPIRDWATCGDIHNLGVKWHIPSDDGVACVKELLDIYYKPEIEKLQNYIKGESISREKLLQSLTILFNVLTGAGVLMPKWKRPSIPELSESQVHMHTLDRTLGSTDKQKRFEGIREELAQVMHDLLNHLLATGEDDTKSIILIININNALMWYMGAEKKDFDSRWKGFLIIKKAMEDRLHGKKRHLRALLIDRVYLQHEMRMLVQAKHVYTDLDKQIHSDLFTLSTSRYSEVRKKAQIMLFKMMDHFVSSVRLIIPKVLVNLKPDTDIPHHQFKGALYVLSGYSMNSTWACLPYWSILKDVWLGLVNAGHSEKLSIMKVLAQVMMKVNKVFDTSAITVKVSDGARSKANDLVQGLNVSITPEQESASVQRLQEHNQSNMRVYNELVAELVDVIEQGKLRWKFSQMAASLLRLQLRVDTPPPIPVVRLFTSLLVHDTLNLRKLAISAMGAIMKQLKHPHKKVPLDPYATAGCPAPPDDALPNPGERPDNKWTQYQCDRLPTTQEKWDQCVFVDKTHWGYYKWPKNMQIYAPFNEQPKLDRSREELSETEQIIYDHFSNAEFVEKLVGYLSLEDKKGRDKFDNKKYTVFKGLFRNFGDTFLPVFQPHIERLSKEALESSQRSMMEMMAGVIRGSKHWGFQKTKNLWETVLLPSMKTSLSLVTAETIIDWGMCMATSCESRDPRKLHWLFEWLTQQPISEDEERGSFLDSSRLFILQGGLFQQEWRVPGLHHRLLGYIRQHLTHSYKNVRDKIGSFLVMIFMYDNAWSRENQTSSPHRQEFLESILPQMAPLANIDVWGAIQVSDEEKAEDDERKKVIRLCKTVMKWIVGSLGNLVSPAPQEYFQYLPLFAPLESCESDEELRYEAEMTLACLAQTLLQPGMIPLVLSSLQQIGQGQSWRAKCSMLEYLQVMVFYNLFTINSLPKHIETIGQLVLDRLKDDRLEVREVAGATLSGLIHCGFLPMENGMQSHFENLCKTRLSTKKVVSMAAPVEAIVQRHAGILGLSACVQAYPYDVPEWMPRILMDLTDHLHDPQPIEKTVRKTLSDFRRTHHDNWHEHKQSFTDDQLVVLTDLLVSPNYYA

Target ORF information:

RefSeq Version	XM_011675563.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus proteasome activator complex subunit 4 (LOC590127), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011675563.1

ORF Insert Sequence:

ATGGATGAGC AAGACGGGAA AGATCCCCGC TACCAAAAAG AATTGATTTA CAACAAATTG 
CTACCGTACT CCTCAGAATT TGAGGAGGAA TCGGAGAGAC ATCTGACTGA AATCAAAACC 
AATTTATCGC TTGCTGTGCA GCTTCGTGAA CTTCGCCCTG GTGTGCTTTT TTGGACCGGG 
AAGTTTTCGG CGTATATACG TTATTATGGC CGAAAGTTCA GCAAGGAAGA GCATGTTGCC 
TTCATCAAGC TCTATCTGGA ACTTATCAAC ATTCCAGATT TGGAATTCAA GATCATGTCA 
GAGTTTGCCA GAATTCTGAA CATACTTCTC AAGAAGCGAA GTTTGCTGTC ACGAGACGAC 
CTGCTTATAC CCTGGCGACC CCTCTACAAG ATTGTTGAAA GGATCATGTA CTCACCATAT 
GAACCATTGG GCTTAGAATG GTATCCACAC ACCCTGGAAA ATCGTCTAAA GTCCCTCATG 
CGGATGTCCA GACCATATTT CTCAGTTGAC AGTACTAGAG AAATGCTAGA GGAATGGCGC 
CCTCTCTTGT GTCCGTTTGA TGAGACCATG GCCAAGGGTA TCCATTACAT GGAGTGCTTT 
CTCCCAACCC TGCTTCCACC AGAACATCAC CAACATGGCT TCAAGTTATG GCTGGATGAA 
ATGATCAGTA TATGGAACAA TTCGCAGAAC TCTCCGCACA TAGAATCAGC ACTAGTAGAC 
CTATTTTCTA GATTAGCTAC AGAGAATATA GGATACATCG ACTGGAATCC ACATATACCA 
AAGATATTCA CATGTTTCTT GAGAGGCCTT AGTCTACCTG TTGGTTCCAA GAAGCTTCAG 
GTAGCTCGGA ATGGGAAGGG TTTTGATGTT GGTCCTCTTG CAACCTGGAT TGTTTCAATG 
CTGGGTCACA CAACAACTTG TCAGGACCAC TTACATAAGT TCTTTGTAGC AACAGATTCA 
TACTTCCATC CATCCAATAC TGGAAAATAC TCTTTCAGCC TTCAAAGGAT GCTTGGTCAG 
CTATCCAGTG AATTTGTCAA GAGATTACAC AGAGAGAGGT ATAAGAAGCC CTCGTGGCAG 
CACCAGATCC CTGATGATTA CAAGCTGAAA GAGGATGACA TCACAGCGTT TGTTGAGAGC 
ATCAAGGCTC CGGTGTTCCT AGCCATGTAC AGTAAGATGG GTCACAGGGA TGCTGCCTCG 
ACCCTGCATA ACCTGGCCTT GCTGAGACCT GAGCTGGTCA TACCAACACT GCTGGAAAAG 
ACCTACAAGG CTTTAGATAC CCTGACAGAG CCCCACCAGC TGATAGCCAC AGTGAGCTGC 
GTGGTGTCGG TCTCCTGCAC CATGCTCAAG GGGGGCAAGT GGCTCCCCGA TGGCCCCACT 
CACCTACTAC CTCTCCTCCA TGCGATGCTA CCTGGTATCG ATCCCAACGA CTTTGCCAAA 
TGCATGGTCA CACTGCAAGC CATCTCTACC TTCACCGTCA TGGTACCCAT AGTAGACTGC 
TCTGAGGCTT CCCTGGTTAG AGACGACATC ACGGAACAGG AGAGGGAGCT CTGTTCAGCA 
ACAGCCACCT TTGAAGATTT TGTGCTTCTC TTCATGGACA GATGTTTCAA TCTGATTGAG 
GCATGTTCCA TCGAGCATAT ACGAAGCCAG GACAACGATC AAGAAAAGGT GAACCACCAA 
GAAGCCATGG TGGACGTAGG ATTGTCTTCA ACATTCAGTA CCATCTGCTC CCAGAGCTCA 
CCTCAGATAT ACAAGGCAGC AGTCAATCGT TTGTTCCGCT ATGTTTCGTC GTCTATCCTT 
GAGACTAAGG TGTCTGGTAA AACCATTGCC AACATGTGCA GGGCAGCCGC TAAGGTGGAT 
CCCAAGTACA CTCTGTCCAA GTTTGTGCCT CACTGTAGCA AGAAGATCCT TGATGCCTTC 
ACATCAGAAG AGGTCCAGTT GGAAGAAGAG TTGGACAAGG AGATTCTCTT CAACCTCCAA 
GTTCTCTCAG AGGTTATACG AGTTCCTAGC GAGGTTGTCC TCACCTATAA GGACACCCTG 
AAAAAGGTCC TGAAGGCTAC CCTTCATCTG ACCTCTAAGA AAGGTTACGA GCTGTCCTGC 
CAGCTACTCA AGCACTGGCT ACGAGCTATG TGCCTCATGT ATCCTAGGGA GTACTGCAGT 
ATAGCAGAGG GCTTTGACAG GCCACTCACA GAGTACTTGC CTATCAGGGA CTGGGCAACG 
TGTGGTGACA TTCACAACCT GGGTGTGAAG TGGCATATAC CCTCTGATGA CGGAGTAGCA 
TGTGTGAAGG AGCTCTTAGA TATCTATTAC AAGCCAGAGA TAGAGAAACT ACAGAACTAC 
ATCAAGGGGG AGTCTATAAG CAGAGAGAAG CTACTACAGA GCCTGACCAT CTTGTTCAAT 
GTACTGACAG GGGCAGGGGT TCTTATGCCT AAATGGAAGA GGCCCTCGAT ACCAGAGCTG 
AGTGAGAGTC AGGTTCACAT GCACACTCTA GATAGGACAC TAGGATCAAC GGACAAACAG 
AAGAGGTTTG AAGGCATCAG AGAAGAACTA GCTCAGGTGA TGCATGATCT CCTTAATCAT 
CTACTAGCAA CAGGGGAGGA TGACACCAAG TCTATTATAC TCATCATCAA TATCAACAAT 
GCGCTGATGT GGTACATGGG AGCAGAGAAG AAGGACTTTG ACAGTCGATG GAAAGGATTC 
CTCATCATCA AGAAAGCCAT GGAAGACAGG TTACATGGGA AGAAGAGGCA CCTGCGAGCC 
CTGTTGATAG ATCGGGTATA CCTCCAGCAT GAGATGAGGA TGCTGGTCCA GGCCAAGCAT 
GTTTACACTG ATCTTGATAA ACAGATTCAT AGTGATCTGT TCACACTGTC AACAAGCAGG 
TACAGCGAGG TGCGTAAGAA GGCCCAGATC ATGCTGTTCA AGATGATGGA TCACTTTGTA 
TCATCAGTGA GGCTAATCAT TCCTAAGGTC TTGGTCAACC TCAAGCCAGA TACAGACATC 
CCACATCACC AGTTCAAGGG AGCCCTCTAT GTACTAAGTG GGTACAGCAT GAACAGCACA 
TGGGCATGCC TGCCTTACTG GTCAATCCTG AAAGACGTAT GGTTAGGACT GGTCAATGCC 
GGCCACTCTG AGAAGCTGTC CATCATGAAG GTCTTAGCTC AGGTCATGAT GAAGGTCAAC 
AAGGTCTTTG ACACGTCGGC CATCACGGTC AAGGTTTCAG ATGGCGCCAG GAGCAAGGCC 
AATGATTTGG TACAGGGTTT GAACGTCAGC ATAACGCCTG AGCAAGAATC AGCATCAGTA 
CAGAGGTTAC AGGAACACAA TCAAAGCAAC ATGAGAGTGT ATAATGAGCT GGTAGCAGAG 
CTGGTGGATG TAATAGAGCA AGGTAAACTG CGATGGAAGT TTTCTCAAAT GGCGGCAAGC 
CTCCTCCGTC TTCAGCTGAG GGTAGACACC CCTCCACCAA TCCCTGTCGT ACGGCTCTTC 
ACATCCCTCC TTGTACATGA CACTCTTAAC CTAAGAAAGC TTGCCATTTC AGCCATGGGT 
GCAATAATGA AGCAACTGAA GCATCCCCAC AAGAAGGTGC CCCTGGACCC CTACGCCACA 
GCTGGGTGCC CCGCCCCTCC TGACGACGCC CTACCCAACC CCGGGGAGCG GCCGGACAAC 
AAGTGGACCC AGTACCAGTG TGACAGGTTG CCCACCACCC AGGAGAAATG GGACCAGTGT 
GTCTTTGTGG ACAAGACTCA CTGGGGGTAC TATAAATGGC CCAAGAACAT GCAGATCTAC 
GCCCCATTCA ATGAACAGCC CAAGCTAGAC CGTAGCAGAG AGGAGCTGTC AGAGACGGAA 
CAGATCATCT ATGATCACTT CAGCAATGCA GAGTTTGTAG AGAAACTAGT AGGCTACCTG 
TCCTTAGAAG ATAAGAAAGG AAGAGACAAG TTTGACAACA AGAAATACAC AGTCTTCAAG 
GGACTGTTCC GTAACTTTGG GGACACTTTC CTGCCAGTCT TCCAGCCCCA CATAGAGAGG 
TTGTCCAAGG AGGCACTGGA GAGTAGCCAG CGGAGTATGA TGGAGATGAT GGCTGGTGTT 
ATACGAGGCA GCAAGCACTG GGGCTTTCAA AAGACCAAGA ATCTGTGGGA GACTGTGCTG 
CTACCTTCTA TGAAGACATC ATTGTCCTTG GTAACAGCAG AGACCATCAT AGATTGGGGG 
ATGTGCATGG CTACGTCATG TGAGAGTAGA GACCCTCGAA AGCTTCATTG GCTGTTTGAA 
TGGCTCACCC AACAACCAAT CAGTGAAGAT GAGGAGAGGG GGTCTTTCCT GGATTCAAGT 
CGTCTGTTCA TCCTACAAGG TGGATTGTTT CAGCAGGAGT GGAGAGTACC AGGTCTTCAT 
CACAGACTGT TAGGCTACAT ACGACAGCAT CTTACCCACA GCTACAAGAA TGTCAGGGAC 
AAGATCGGCA GCTTCCTGGT CATGATCTTC ATGTACGACA ATGCCTGGTC CCGGGAGAAC 
CAGACATCCT CCCCTCACCG CCAAGAGTTC TTAGAATCCA TCCTACCCCA GATGGCCCCC 
CTGGCTAACA TAGACGTCTG GGGAGCTATC CAGGTCTCTG ATGAAGAGAA GGCAGAGGAT 
GATGAGAGGA AGAAGGTCAT TAGGTTATGT AAAACAGTCA TGAAGTGGAT TGTAGGTAGC 
CTAGGGAACC TTGTATCTCC TGCACCTCAA GAATACTTCC AGTACCTTCC TCTGTTTGCA 
CCTCTTGAGA GCTGTGAGTC TGATGAAGAG CTACGGTATG AAGCAGAGAT GACCCTAGCC 
TGTCTAGCCC AGACTTTACT TCAACCAGGG ATGATACCCC TTGTACTCTC CTCTCTACAG 
CAGATTGGTC AGGGCCAATC CTGGAGAGCC AAGTGTAGTA TGTTAGAGTA CCTTCAGGTG 
ATGGTGTTCT ACAACCTCTT CACCATCAAC AGTCTACCCA AGCATATAGA AACCATTGGT 
CAACTCGTAC TGGACAGGCT CAAGGACGAT CGCCTTGAGG TGAGAGAGGT AGCAGGTGCA 
ACACTGAGCG GTCTGATTCA CTGTGGCTTC TTACCAATGG AGAATGGGAT GCAGAGCCAC 
TTTGAGAACC TGTGTAAGAC AAGACTATCG ACCAAGAAAG TAGTCAGTAT GGCTGCACCC 
GTTGAAGCCA TAGTCCAGAG GCATGCTGGT ATCCTGGGTC TGAGTGCATG TGTCCAAGCC 
TACCCCTATG ATGTCCCTGA ATGGATGCCA AGGATACTGA TGGACCTCAC AGATCATCTA 
CACGATCCTC AACCTATCGA GAAAACAGTG CGAAAAACCC TGTCTGACTT CCGCAGAACG 
CATCACGACA ACTGGCACGA GCACAAGCAG AGCTTCACAG ACGACCAGCT TGTAGTACTT 
ACTGACCTTC TAGTCTCGCC CAACTACTAC GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC590127 ( XM_030999469.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030855329.1 Strongylocentrotus purpuratus proteasome activator complex subunit 4 (LOC590127), mRNA.

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CloneID	OSd65375
Clone ID Related Accession (Same CDS sequence)	XM_030999469.1
Accession Version	XM_030999469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	proteasome activator complex subunit 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGC AAGACGGGAA AGATCCCCGC TACCAAAAAG AATTGATTTA CAACAAATTG 
CTACCGTACT CCTCGGAATT TGAGGAGGAA TCGGAGAGAC ATCTGACTGA AATCAAAACC 
AATTTATCGC TTGCTGTGCA GCTTCGTGAA CTTCGCCCTG GTGTGCTTTT TTGGACCGGG 
AAGTTTTCGG CGTATATACG TTATTATGGC CGAAAGTTCA GCAAGGAAGA GCATGTGGCC 
TTCATCAAGC TCTATCTGGA ACTTATCAAC ATTCCAGATT TGGAGTTCAA GATCATGTCA 
GAGTTTGCCA GAATTCTGAA CATACTTCTC AAGAAGCGAA GTTTGCTGTC ACGAGACGAC 
CTGCTTATAC CCTGGCGACC CCTCTACAAG ATTGTTGAAA GGATCATGTA CTCACCATAT 
GAACCATTGG GCTTAGAATG GTATCCACAC ACCCTGGAAA ATCGTCTAAA GTCCCTCATG 
CGGATGTCCA GACCATATTT CTCAGTTGAC AGTACTAGAG AAATGCTAGA GGAATGGCGC 
CCTCTCTTGT GTCCGTTTGA TGAGACCATG GCCAAGGGTA TCCATTACAT GGAGTGCTTT 
CTCCCAACCC TGCTTCCACC AGAACATCAC CAACATGGCT TCAAGTTATG GCTGGATGAA 
ATGATCAGTA TATGGAACAA TTCGCAGAAC TCTCCGCACA TAGAATCAGC ACTAGTAGAC 
CTATTTTCTA GATTAGCTAC AGAGAATATA GGATACATTG ACTGGAATCC TCATATACCA 
AAGATATTCA CATGTTTCTT GAGAGGCCTT AGTCTACCTG TTGGTTCCAA GAAGCTTCAG 
GTAGCTCGGA ATGGGAAGGG TTTTGATGTT GGTCCTCTTG CAACCTGGAT TGTTTCAATG 
CTGGGTCACA CAACAACTTG TCAGGACCAC TTACATAAGT TCTTTGTAGC AACAGATTCA 
TACTTCCATC CATCCAATAC TGGAAAATAC TCTTTCAGCC TTCAAAGGAT GCTTGGTCAG 
CTATCCAGTG AATTTGTCAA GAGATTACAC AGAGAGAGGT ATAAGAAGCC CTCGTGGCAG 
CACCAGATCC CTGATGATTA CAAGCTGAAA GAGGATGACA TCACAGCGTT TGTTGAGAGC 
ATCAAGGCTC CGGTGTTCCT AGCCATGTAC AGTAAGATGG GTCACAGGGA TGCTGCCTCG 
ACCCTGCATA ACCTGGCCTT GCTGAGACCT GAGCTGGTCA TACCAACACT GCTGGAAAAG 
ACCTACAAGG CTTTAGATAC CCTGACAGAG CCCCACCAGC TGATAGCCAC AGTGAGCTGC 
GTGGTGTCGG TCTCCTGCAC CATGCTCAAG GGGGGCAAGT GGCTCCCCGA TGGCCCCACT 
CACCTACTAC CTCTCCTCCA TGCGATGCTA CCTGGTATCG ATCCCAACGA CTTTGCCAAA 
TGCATGGTCA CACTGCAAGC CATCTCTACC TTCACCGTCA TGGTACCCAT AGTAGACTGC 
TCTGAAGCTT CCCTGGTTAG AGACGACATC ACGGAACAGG AGAGGGAGCT CTGTTCAGCA 
ACAGCCACCT TTGAAGATTT TGTGCTTCTC TTCATGGACA GATGTTTCAA TCTGATTGAG 
GCATGTTCCA TCGAGCATAT ACGAAGCCAG GACAACGATC AAGAAAAGGT GAACCACCAA 
GAAGCCATGG TGGACGTAGG ATTGTCTTCA ACATTCAGTA CCATCTGCTC CCAGAGCTCA 
CCTCAGATAT ACAAGGCAGC AGTCAATCGT TTGTTCCGCT ATGTTTCGTC GTCTATCCTT 
GAGACTAAGG TGTCTGGTAA AACCATTGCC AACATGTGCA GGGCAGCCGC TAAGGTGGAT 
CCCAAGTACA CTCTGTCCAA GTTTGTGCCT CACTGTAGCA AGAAGATCCT TGATGCCTTC 
ACATCAGAAG AGGTCCAGTT GGAAGAAGAG TTGGACAAGG AGATTCTCTT CAACCTCCAA 
GTTCTCTCAG AGGTTATACG AGTTCCTAGC GAGGTTGTCC TCACCTATAA GGACACCCTG 
AAAAAGGTCC TGAAGGCTAC CCTTCATCTG ACCTCTAAGA AAGGTTACGA GCTGTCCTGC 
CAGCTACTCA AGCACTGGCT ACGAGCTATG TGCCTCATGT ATCCTAGGGA GTACTGCAGT 
ATAGCAGAGG GCTTTGACAG GCCACTCACA GAGTACTTGC CTATCAGGGA CTGGGCAACG 
TGCGGTGACA TTCACAACCT GGGTGTGAAG TGGCATATAC CCTCTGATGA CGGAGTAGCA 
TGTGTGAAGG AGCTCTTAGA TATCTATTAC AAGCCAGAGA TAGAGAAACT ACAGAACTAC 
ATCAAGGGGG AGTCTATAAG CAGAGAGAAG CTACTACAGA GCCTGACCAT CTTGTTCAAT 
GTACTGACAG GGGCAGGGGT TCTTATGCCT AAATGGAAGA GGCCCTCGAT ACCAGAGCTG 
AGTGAGAGTC AAGTTCACAT GCACACTCTA GATAGGACAC TAGGATCAAC GGACAAACAG 
AAGAGGTTTG AAGGCATCAG AGAAGAACTA GCTCAGGTGA TGCATGATCT TCTTAATCAT 
CTCCTAGCAA CAGGGGAGGA TGACACCAAG TCCATCATAC TCATCATCAA TATCAACAAT 
GCGCTGATGT GGTACATGGG AGCAGAGAAG AAGGACTTTG ACAGCCGATG GAAGGGATTC 
CTCATCATCA AGAAAGCCAT GGAAGACAGG TTACATGGGA AGAAGAGGCA CCTGCGAGCC 
CTGTTGATAG ATCGGGTATA CCTCCAGCAT GAGATGAGGA TGCTGGTCCA GGCCAAGCAT 
GTTTACACTG ATCTTGATAA ACAGATTCAT AGTGATCTGT TCACACTGTC AACAAGCAGG 
TACAGCGAGG TGCGTAAGAA GGCCCAGATC ATGCTGTTCA AGATGATGGA TCACTTTGTA 
TCATCAGTGA GGCTAATCAT TCCTAAGGTC TTGGTCAACC TCAAGCCAGA TACAGACATC 
CCACATCACC AGTTCAAGGG AGCCCTCTAT GTACTAAGTG GGTACAGCAT GAACAGCACA 
TGGGCATGCC TGCCTTACTG GTCAATCCTG AAAGACGTAT GGTTAGGACT GGTCAATGCC 
GGCCACTCTG AGAAGCTGTC CATCATGAAG GTCTTAGCTC AGGTCATGAT GAAGGTCAAC 
AAGGTCTTTG ACACGTCGGC CATCACGGTC AAGGTTTCAG ATGGCGCCAG GAGCAAGGCC 
AATGATTTGG TACAGGGTTT GAACGTCAGC ATAACGCCTG AGCAAGAATC AGCATCAGTA 
CAGAGGTTAC AGGAACACAA TCAAAGCAAC ATGAGAGTGT ATAATGAGCT GGTATCCGAG 
CTGGTGGATG TAATAGAGCA AGGCAAACTG CGATGGAAGT TTTCTCAAAT GGCGGCAAGC 
CTCCTCCGTC TTCAGCTGAG GGTAGATACC CCTCCACCAA TCCCTGTCGT ACGGCTCTTC 
ACATCCCTCC TCGTACATGA CACACTTAAC TTAAGAAAGC TTGCCATTTC AGCCATGGGT 
GCAATAATGA AGCAACTGAA GCGTCCGCAC AAGAAGGTGC CCCTTGACCC CTACGCAACA 
GCTGGGTGCC CCGCCCCTCC TGACGACGCC CTACCCAACC CCGGGGAGCG GCCGGACAAC 
AAGTGGACTC AGTACCAGTG TGACAGGTTG CCCACCACCC AGGAGAAATG GGACCAGTGT 
GTGTTTGTGG ATAAGACCCA CTGGGGCTAC TATAAATGGC CCAAGAACAT GCAGATCTAC 
GCCCCATTCG ATGAACAGCC CAAGCTAGAC CGTAGCAGAG AGGAGCTGTC AGAGACGGAA 
CAGATCATCT ATGATCACTT CAGCAATGCA GAGTTTGTAG AGAAACTAGT AGGCTACCTG 
TCCTTAGAAG ATAAGAAAGG AAGAGACAAG TTTGACAACA AGAAATACAC AGTCTTCAAG 
GGACTGTTCC GTAACTTTGG GGACACTTTC CTGCCAGTCT TCCAGCCCCA CATAGAGAGG 
TTGTCCAAGG AGGCACTGGA GAGTAGCCAG CGGAGTATGA TGGAGATGAT GGCTGGTGTT 
ATACGAGGCA GCAAGCACTG GGGCTTTCAA AAGACCAAGA ATCTGTGGGA GACTGTGCTG 
CTACCTTCTA TGAAGACATC ATTGTCCTTG GTAACAGCAG AGACCATCAT AGATTGGGGG 
ATGTGCATGG CTACGTCATG TGAGAGTAGA GACCCTCGAA AGCTTCATTG GCTGTTTGAA 
TGGCTCACCC AACAACCAAT CAGTGAAGAT GAGGAGAGGG GGTCTTTCCT GGATTCAAGT 
CGTCTGTTCA TCCTACAAGG TGGATTGTTT CAGCAGGAGT GGAGAGTACC AGGTCTTCAT 
CACAGACTGT TAGGCTACAT ACGACAGCAT CTTACCCACA GCTACAAGAA TGTCAGGGAC 
AAGATCGGCA GCTTCCTGGT CATGATCTTC ATGTACGACA ATGCCTGGTC CCGGGAGAAC 
CAGACATCCT CCCCTCACCG CCAAGAGTTC TTAGAATCCA TCCTACCCCA GATGGCCCCC 
CTGGCTAACA TAGACGTCTG GGGAGCTATC CAGGTCTCTG ATGAAGAGAA GGCAGAGGAT 
GATGAGAGGA AGAAGGTCAT TAGGTTATGT AAAACAGTCA TGAAGTGGAT TGTAGGTAGC 
CTAGGGAACC TTGTATCTCC TGCACCTCAA GAATACTTCC AGTACCTTCC TCTGTTTGCA 
CCTCTTGAGA GCTGTGAGTC TGATGAAGAG CTACGGTATG AAGCAGAGAT GACCCTAGCC 
TGTCTAGCCC AGACTTTACT TCAACCAGGG ATGATACCCC TTGTACTCTC CTCTCTACAG 
CAGATTGGTC AGGGCCAATC CTGGAGAGCC AAGTGTAGTA TGTTAGAGTA CCTTCAGGTG 
ATGGTGTTCT ACAACCTCTT CACCATCAAC AGTCTACCCA AGCATATAGA AACCATTGGT 
CAACTCGTAC TGGACAGGCT CAAGGACGAT CGCCTTGAGG TGAGAGAGGT AGCAGGTGCA 
ACACTGAGCG GTCTGATTCA CTGTGGCTTC TTACCAATGG AGAATGGGAT GCAGAGCCAC 
TTTGAGAACC TGTGTAAGAC AAGACTATCG ACCAAGAAAG TAGTCAGTAT GGCTGCACCC 
GTTGAAGCCA TAGTCCAGAG GCATGCTGGT ATCCTGGGTC TGAGTGCATG TGTCCAAGCC 
TACCCCTATG ATGTCCCTGA ATGGATGCCA AGGATACTGA TGGACCTCAC AGATCATCTA 
CACGATCCTC AACCTATCGA GAAAACAGTG CGAAAAACCC TGTCTGACTT CCGCAGAACG 
CATCACGACA ACTGGCACGA GCACAAGCAG AGCTTCACAG ACGACCAGCT TGTAGTACTT 
ACTGACCTTC TAGTCTCGCC CAACTACTAC GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030855329.1
CDS	129..5564
Translation	MDEQDGKDPRYQKELIYNKLLPYSSEFEEESERHLTEIKTNLSLAVQLRELRPGVLFWTGKFSAYIRYYGRKFSKEEHVAFIKLYLELINIPDLEFKIMSEFARILNILLKKRSLLSRDDLLIPWRPLYKIVERIMYSPYEPLGLEWYPHTLENRLKSLMRMSRPYFSVDSTREMLEEWRPLLCPFDETMAKGIHYMECFLPTLLPPEHHQHGFKLWLDEMISIWNNSQNSPHIESALVDLFSRLATENIGYIDWNPHIPKIFTCFLRGLSLPVGSKKLQVARNGKGFDVGPLATWIVSMLGHTTTCQDHLHKFFVATDSYFHPSNTGKYSFSLQRMLGQLSSEFVKRLHRERYKKPSWQHQIPDDYKLKEDDITAFVESIKAPVFLAMYSKMGHRDAASTLHNLALLRPELVIPTLLEKTYKALDTLTEPHQLIATVSCVVSVSCTMLKGGKWLPDGPTHLLPLLHAMLPGIDPNDFAKCMVTLQAISTFTVMVPIVDCSEASLVRDDITEQERELCSATATFEDFVLLFMDRCFNLIEACSIEHIRSQDNDQEKVNHQEAMVDVGLSSTFSTICSQSSPQIYKAAVNRLFRYVSSSILETKVSGKTIANMCRAAAKVDPKYTLSKFVPHCSKKILDAFTSEEVQLEEELDKEILFNLQVLSEVIRVPSEVVLTYKDTLKKVLKATLHLTSKKGYELSCQLLKHWLRAMCLMYPREYCSIAEGFDRPLTEYLPIRDWATCGDIHNLGVKWHIPSDDGVACVKELLDIYYKPEIEKLQNYIKGESISREKLLQSLTILFNVLTGAGVLMPKWKRPSIPELSESQVHMHTLDRTLGSTDKQKRFEGIREELAQVMHDLLNHLLATGEDDTKSIILIININNALMWYMGAEKKDFDSRWKGFLIIKKAMEDRLHGKKRHLRALLIDRVYLQHEMRMLVQAKHVYTDLDKQIHSDLFTLSTSRYSEVRKKAQIMLFKMMDHFVSSVRLIIPKVLVNLKPDTDIPHHQFKGALYVLSGYSMNSTWACLPYWSILKDVWLGLVNAGHSEKLSIMKVLAQVMMKVNKVFDTSAITVKVSDGARSKANDLVQGLNVSITPEQESASVQRLQEHNQSNMRVYNELVSELVDVIEQGKLRWKFSQMAASLLRLQLRVDTPPPIPVVRLFTSLLVHDTLNLRKLAISAMGAIMKQLKRPHKKVPLDPYATAGCPAPPDDALPNPGERPDNKWTQYQCDRLPTTQEKWDQCVFVDKTHWGYYKWPKNMQIYAPFDEQPKLDRSREELSETEQIIYDHFSNAEFVEKLVGYLSLEDKKGRDKFDNKKYTVFKGLFRNFGDTFLPVFQPHIERLSKEALESSQRSMMEMMAGVIRGSKHWGFQKTKNLWETVLLPSMKTSLSLVTAETIIDWGMCMATSCESRDPRKLHWLFEWLTQQPISEDEERGSFLDSSRLFILQGGLFQQEWRVPGLHHRLLGYIRQHLTHSYKNVRDKIGSFLVMIFMYDNAWSRENQTSSPHRQEFLESILPQMAPLANIDVWGAIQVSDEEKAEDDERKKVIRLCKTVMKWIVGSLGNLVSPAPQEYFQYLPLFAPLESCESDEELRYEAEMTLACLAQTLLQPGMIPLVLSSLQQIGQGQSWRAKCSMLEYLQVMVFYNLFTINSLPKHIETIGQLVLDRLKDDRLEVREVAGATLSGLIHCGFLPMENGMQSHFENLCKTRLSTKKVVSMAAPVEAIVQRHAGILGLSACVQAYPYDVPEWMPRILMDLTDHLHDPQPIEKTVRKTLSDFRRTHHDNWHEHKQSFTDDQLVVLTDLLVSPNYYA

Target ORF information:

RefSeq Version	XM_030999469.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus proteasome activator complex subunit 4 (LOC590127), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030999469.1

ORF Insert Sequence:

ATGGATGAGC AAGACGGGAA AGATCCCCGC TACCAAAAAG AATTGATTTA CAACAAATTG 
CTACCGTACT CCTCGGAATT TGAGGAGGAA TCGGAGAGAC ATCTGACTGA AATCAAAACC 
AATTTATCGC TTGCTGTGCA GCTTCGTGAA CTTCGCCCTG GTGTGCTTTT TTGGACCGGG 
AAGTTTTCGG CGTATATACG TTATTATGGC CGAAAGTTCA GCAAGGAAGA GCATGTGGCC 
TTCATCAAGC TCTATCTGGA ACTTATCAAC ATTCCAGATT TGGAGTTCAA GATCATGTCA 
GAGTTTGCCA GAATTCTGAA CATACTTCTC AAGAAGCGAA GTTTGCTGTC ACGAGACGAC 
CTGCTTATAC CCTGGCGACC CCTCTACAAG ATTGTTGAAA GGATCATGTA CTCACCATAT 
GAACCATTGG GCTTAGAATG GTATCCACAC ACCCTGGAAA ATCGTCTAAA GTCCCTCATG 
CGGATGTCCA GACCATATTT CTCAGTTGAC AGTACTAGAG AAATGCTAGA GGAATGGCGC 
CCTCTCTTGT GTCCGTTTGA TGAGACCATG GCCAAGGGTA TCCATTACAT GGAGTGCTTT 
CTCCCAACCC TGCTTCCACC AGAACATCAC CAACATGGCT TCAAGTTATG GCTGGATGAA 
ATGATCAGTA TATGGAACAA TTCGCAGAAC TCTCCGCACA TAGAATCAGC ACTAGTAGAC 
CTATTTTCTA GATTAGCTAC AGAGAATATA GGATACATTG ACTGGAATCC TCATATACCA 
AAGATATTCA CATGTTTCTT GAGAGGCCTT AGTCTACCTG TTGGTTCCAA GAAGCTTCAG 
GTAGCTCGGA ATGGGAAGGG TTTTGATGTT GGTCCTCTTG CAACCTGGAT TGTTTCAATG 
CTGGGTCACA CAACAACTTG TCAGGACCAC TTACATAAGT TCTTTGTAGC AACAGATTCA 
TACTTCCATC CATCCAATAC TGGAAAATAC TCTTTCAGCC TTCAAAGGAT GCTTGGTCAG 
CTATCCAGTG AATTTGTCAA GAGATTACAC AGAGAGAGGT ATAAGAAGCC CTCGTGGCAG 
CACCAGATCC CTGATGATTA CAAGCTGAAA GAGGATGACA TCACAGCGTT TGTTGAGAGC 
ATCAAGGCTC CGGTGTTCCT AGCCATGTAC AGTAAGATGG GTCACAGGGA TGCTGCCTCG 
ACCCTGCATA ACCTGGCCTT GCTGAGACCT GAGCTGGTCA TACCAACACT GCTGGAAAAG 
ACCTACAAGG CTTTAGATAC CCTGACAGAG CCCCACCAGC TGATAGCCAC AGTGAGCTGC 
GTGGTGTCGG TCTCCTGCAC CATGCTCAAG GGGGGCAAGT GGCTCCCCGA TGGCCCCACT 
CACCTACTAC CTCTCCTCCA TGCGATGCTA CCTGGTATCG ATCCCAACGA CTTTGCCAAA 
TGCATGGTCA CACTGCAAGC CATCTCTACC TTCACCGTCA TGGTACCCAT AGTAGACTGC 
TCTGAAGCTT CCCTGGTTAG AGACGACATC ACGGAACAGG AGAGGGAGCT CTGTTCAGCA 
ACAGCCACCT TTGAAGATTT TGTGCTTCTC TTCATGGACA GATGTTTCAA TCTGATTGAG 
GCATGTTCCA TCGAGCATAT ACGAAGCCAG GACAACGATC AAGAAAAGGT GAACCACCAA 
GAAGCCATGG TGGACGTAGG ATTGTCTTCA ACATTCAGTA CCATCTGCTC CCAGAGCTCA 
CCTCAGATAT ACAAGGCAGC AGTCAATCGT TTGTTCCGCT ATGTTTCGTC GTCTATCCTT 
GAGACTAAGG TGTCTGGTAA AACCATTGCC AACATGTGCA GGGCAGCCGC TAAGGTGGAT 
CCCAAGTACA CTCTGTCCAA GTTTGTGCCT CACTGTAGCA AGAAGATCCT TGATGCCTTC 
ACATCAGAAG AGGTCCAGTT GGAAGAAGAG TTGGACAAGG AGATTCTCTT CAACCTCCAA 
GTTCTCTCAG AGGTTATACG AGTTCCTAGC GAGGTTGTCC TCACCTATAA GGACACCCTG 
AAAAAGGTCC TGAAGGCTAC CCTTCATCTG ACCTCTAAGA AAGGTTACGA GCTGTCCTGC 
CAGCTACTCA AGCACTGGCT ACGAGCTATG TGCCTCATGT ATCCTAGGGA GTACTGCAGT 
ATAGCAGAGG GCTTTGACAG GCCACTCACA GAGTACTTGC CTATCAGGGA CTGGGCAACG 
TGCGGTGACA TTCACAACCT GGGTGTGAAG TGGCATATAC CCTCTGATGA CGGAGTAGCA 
TGTGTGAAGG AGCTCTTAGA TATCTATTAC AAGCCAGAGA TAGAGAAACT ACAGAACTAC 
ATCAAGGGGG AGTCTATAAG CAGAGAGAAG CTACTACAGA GCCTGACCAT CTTGTTCAAT 
GTACTGACAG GGGCAGGGGT TCTTATGCCT AAATGGAAGA GGCCCTCGAT ACCAGAGCTG 
AGTGAGAGTC AAGTTCACAT GCACACTCTA GATAGGACAC TAGGATCAAC GGACAAACAG 
AAGAGGTTTG AAGGCATCAG AGAAGAACTA GCTCAGGTGA TGCATGATCT TCTTAATCAT 
CTCCTAGCAA CAGGGGAGGA TGACACCAAG TCCATCATAC TCATCATCAA TATCAACAAT 
GCGCTGATGT GGTACATGGG AGCAGAGAAG AAGGACTTTG ACAGCCGATG GAAGGGATTC 
CTCATCATCA AGAAAGCCAT GGAAGACAGG TTACATGGGA AGAAGAGGCA CCTGCGAGCC 
CTGTTGATAG ATCGGGTATA CCTCCAGCAT GAGATGAGGA TGCTGGTCCA GGCCAAGCAT 
GTTTACACTG ATCTTGATAA ACAGATTCAT AGTGATCTGT TCACACTGTC AACAAGCAGG 
TACAGCGAGG TGCGTAAGAA GGCCCAGATC ATGCTGTTCA AGATGATGGA TCACTTTGTA 
TCATCAGTGA GGCTAATCAT TCCTAAGGTC TTGGTCAACC TCAAGCCAGA TACAGACATC 
CCACATCACC AGTTCAAGGG AGCCCTCTAT GTACTAAGTG GGTACAGCAT GAACAGCACA 
TGGGCATGCC TGCCTTACTG GTCAATCCTG AAAGACGTAT GGTTAGGACT GGTCAATGCC 
GGCCACTCTG AGAAGCTGTC CATCATGAAG GTCTTAGCTC AGGTCATGAT GAAGGTCAAC 
AAGGTCTTTG ACACGTCGGC CATCACGGTC AAGGTTTCAG ATGGCGCCAG GAGCAAGGCC 
AATGATTTGG TACAGGGTTT GAACGTCAGC ATAACGCCTG AGCAAGAATC AGCATCAGTA 
CAGAGGTTAC AGGAACACAA TCAAAGCAAC ATGAGAGTGT ATAATGAGCT GGTATCCGAG 
CTGGTGGATG TAATAGAGCA AGGCAAACTG CGATGGAAGT TTTCTCAAAT GGCGGCAAGC 
CTCCTCCGTC TTCAGCTGAG GGTAGATACC CCTCCACCAA TCCCTGTCGT ACGGCTCTTC 
ACATCCCTCC TCGTACATGA CACACTTAAC TTAAGAAAGC TTGCCATTTC AGCCATGGGT 
GCAATAATGA AGCAACTGAA GCGTCCGCAC AAGAAGGTGC CCCTTGACCC CTACGCAACA 
GCTGGGTGCC CCGCCCCTCC TGACGACGCC CTACCCAACC CCGGGGAGCG GCCGGACAAC 
AAGTGGACTC AGTACCAGTG TGACAGGTTG CCCACCACCC AGGAGAAATG GGACCAGTGT 
GTGTTTGTGG ATAAGACCCA CTGGGGCTAC TATAAATGGC CCAAGAACAT GCAGATCTAC 
GCCCCATTCG ATGAACAGCC CAAGCTAGAC CGTAGCAGAG AGGAGCTGTC AGAGACGGAA 
CAGATCATCT ATGATCACTT CAGCAATGCA GAGTTTGTAG AGAAACTAGT AGGCTACCTG 
TCCTTAGAAG ATAAGAAAGG AAGAGACAAG TTTGACAACA AGAAATACAC AGTCTTCAAG 
GGACTGTTCC GTAACTTTGG GGACACTTTC CTGCCAGTCT TCCAGCCCCA CATAGAGAGG 
TTGTCCAAGG AGGCACTGGA GAGTAGCCAG CGGAGTATGA TGGAGATGAT GGCTGGTGTT 
ATACGAGGCA GCAAGCACTG GGGCTTTCAA AAGACCAAGA ATCTGTGGGA GACTGTGCTG 
CTACCTTCTA TGAAGACATC ATTGTCCTTG GTAACAGCAG AGACCATCAT AGATTGGGGG 
ATGTGCATGG CTACGTCATG TGAGAGTAGA GACCCTCGAA AGCTTCATTG GCTGTTTGAA 
TGGCTCACCC AACAACCAAT CAGTGAAGAT GAGGAGAGGG GGTCTTTCCT GGATTCAAGT 
CGTCTGTTCA TCCTACAAGG TGGATTGTTT CAGCAGGAGT GGAGAGTACC AGGTCTTCAT 
CACAGACTGT TAGGCTACAT ACGACAGCAT CTTACCCACA GCTACAAGAA TGTCAGGGAC 
AAGATCGGCA GCTTCCTGGT CATGATCTTC ATGTACGACA ATGCCTGGTC CCGGGAGAAC 
CAGACATCCT CCCCTCACCG CCAAGAGTTC TTAGAATCCA TCCTACCCCA GATGGCCCCC 
CTGGCTAACA TAGACGTCTG GGGAGCTATC CAGGTCTCTG ATGAAGAGAA GGCAGAGGAT 
GATGAGAGGA AGAAGGTCAT TAGGTTATGT AAAACAGTCA TGAAGTGGAT TGTAGGTAGC 
CTAGGGAACC TTGTATCTCC TGCACCTCAA GAATACTTCC AGTACCTTCC TCTGTTTGCA 
CCTCTTGAGA GCTGTGAGTC TGATGAAGAG CTACGGTATG AAGCAGAGAT GACCCTAGCC 
TGTCTAGCCC AGACTTTACT TCAACCAGGG ATGATACCCC TTGTACTCTC CTCTCTACAG 
CAGATTGGTC AGGGCCAATC CTGGAGAGCC AAGTGTAGTA TGTTAGAGTA CCTTCAGGTG 
ATGGTGTTCT ACAACCTCTT CACCATCAAC AGTCTACCCA AGCATATAGA AACCATTGGT 
CAACTCGTAC TGGACAGGCT CAAGGACGAT CGCCTTGAGG TGAGAGAGGT AGCAGGTGCA 
ACACTGAGCG GTCTGATTCA CTGTGGCTTC TTACCAATGG AGAATGGGAT GCAGAGCCAC 
TTTGAGAACC TGTGTAAGAC AAGACTATCG ACCAAGAAAG TAGTCAGTAT GGCTGCACCC 
GTTGAAGCCA TAGTCCAGAG GCATGCTGGT ATCCTGGGTC TGAGTGCATG TGTCCAAGCC 
TACCCCTATG ATGTCCCTGA ATGGATGCCA AGGATACTGA TGGACCTCAC AGATCATCTA 
CACGATCCTC AACCTATCGA GAAAACAGTG CGAAAAACCC TGTCTGACTT CCGCAGAACG 
CATCACGACA ACTGGCACGA GCACAAGCAG AGCTTCACAG ACGACCAGCT TGTAGTACTT 
ACTGACCTTC TAGTCTCGCC CAACTACTAC GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC590127 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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