LOC587252 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC587252
Entrez Gene ID	587252
Full Name	laminin subunit gamma-1
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_030997903.1	XP_030853763.1	laminin subunit gamma-1 isoform X1
XM_011672484.1	XP_011670786.1	LOW QUALITY PROTEIN: laminin subunit gamma-1
XM_030997904.1	XP_030853764.1	laminin subunit gamma-1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC587252 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC587252 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd25579	XM_011672484.1 Latest version!	Strongylocentrotus purpuratus laminin subunit gamma-1 (LOC587252), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSd63704	XM_030997903.1 Latest version!	Strongylocentrotus purpuratus laminin subunit gamma-1 (LOC587252), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSd63705	XM_030997904.1 Latest version!	Strongylocentrotus purpuratus laminin subunit gamma-1 (LOC587252), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC587252 ( XM_011672484.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011670786.1 Strongylocentrotus purpuratus laminin subunit gamma-1 (LOC587252), mRNA.

Price & Availability↑

CloneID	OSd25579
Clone ID Related Accession (Same CDS sequence)	XM_011672484.1
Accession Version	XM_011672484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	LOW QUALITY PROTEIN: laminin subunit gamma-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011981913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGGTAAAT CCTTTGAGAT CACATACATT CGGCTGAAAT TTNACTCGCC GCGGCCGGAG 
AGTTTTGCCA TCTACAAGCG TACGTGTCAG GACTGTCCTT GGATACCCTA CCAGTATTAC 
AGCGGCTCAT GTAGAGGAAC CTACACCCTC CCAGATAGCG AATACGTCAG CTACGATGAT 
GAAACATTGG CGCTCTGCAC CAGTGAATTC AGCGACATCT CGCCACTCAC AGGAGGAAAT 
ATTGCTTTCA GTACGCTGGA AAATAGACCG AGTGCAAGGA GTTTTGATAA TAGTGCCGTT 
TTACAGGATT GGGTGACAGC TACAGATATT CTGGTTATGC TGACGAGATT AAACACTTTT 
GGAGATGAAG TTTTCAGGGT ACCAAACGTT CTACGATCAT ACTTCTTTGC CATCACAGAT 
TTCTCTATTG GTGGAAGGTG TAAATGCAAC GGTCACGCCA GCCACTGCGT GGAAGCACCC 
AACGATCCTT ACAACCGTCT TGTATGTCAG TGCGAACACA ACACAGCAGG GCCAGACTGT 
GGCGAATGTC TCCCTCTGTA CAATGACAGG AAGTGGACGA GAGCCACTGC TGGGAACGCA 
AATGAATGCA TGAGATGTAA CTGCAACTTC CACGCTGACC GTTGCTTCTT CGATGAAGAG 
CTTTACAACC GAACAGGATC CGGTGGTCAC TGCATGGAGT GCCGTGATGA AACTTCTGGC 
GTCAGCTGTG AGCGTTGTCG CGACAACTAC TTCAGGACTT CTCCAGACGC CCGATGCCAG 
CCTTGTAACT GTGACCAAAC AGGATCATTG AACCAGCAGT GTGATAGCTC AGGACGATGC 
CAGTGTAAGG TGGGAGTAGG AGGAGATAAA TGTAACCAGT GTCTTCCTAA CTTCTATGAC 
TTTGGTGAAG GTGGATGCAG GAGCTGCGAG TGTGTGCGTG CAGGAAGTGT AGACAACAAC 
CCAGACTGTA ACCAGGGTAC AGGTCTCTGC AACTGTAAAC TTCATGTTGA AGGAAGGGAC 
TGCGAGACCT GCAAGCCTGG TTTCTTCAAC CTTCAAGAGG CAGATGAGTT TGGATGCCTT 
CCCTGCTTCT GTCATGGTCA TTCATCCTCT TGCTCCTCTG CTCCTGGCTA TGACATTGCC 
TACATCGAGT CCAACTTCAA TTCAGGTGTG GATGGTTGGA AAGCCGAGAG TCGAAATGGA 
GACCAGGAAT TGCTTGTTTA TAATGCCATC AGGCAGGACA TCGGTATCGC AACAGTTGAC 
AACCAACCCA TGTACTTTAT AGCCCCAGAT GAGTACCTTG GTAACAAGTT GTTCAGCTAT 
GGTCAGGAGC TGAGCTTCAA GCTGAGACTG GGCAGTGATG ATGTGAGTCT CAGCGTGGAG 
GATCTCGTCT TGGAGGGAGG TGGCAGGCAG ATCTCTGCTC CGCTGGTTGC CCAGGGCAAC 
CCGCGACCAG GTGTCTACCC GCAAAAATTT ACCTTCAAAC TGCGAGCTGA GGATGATATG 
GGATGGGTTC AACGACCTTC AGCCTTCGAG TTCCAGAGCC TCCTTTCCAA CCTGACTGCC 
ATCAAGATCA GAGCAACCTA CAGTGATAGA GGTCAAGGAC TGCTTGATGA CGTCCGTCTG 
GTCAGTGGCC GGCGGTCAGT CGGTCCACAA AGCACCAGGG CTACATTCGT AGAGCTGTGC 
AGCTGCCCAA CCGGCTATGT CGGTCAATTC TGTGAGTCCT GCGCTGCTGG CTACCGCAGG 
GACCCCTACG GCAATGGACC TTTCACACGT TGCATCCCCT GCGAGTGCAA TGGACATGCC 
GACTACTGTG ATGTTGATAC TGGTGAATGT ATCTGTAACC ACAACACCAT GGGAACAAGC 
TGTGAACTAT GCCAGGATGG TTTCTATGGT AACGCCCTTG AGGGCACGGC CTATGACTGC 
ACACGCTGCC CCTGCCCCGG TGGAACCAAC TGCTTTGTCG ATAACCTAGG TGATGTGGTC 
TGTACCGAGT GCCCATCAGG CTATGTTGGA AACCGCTGTG ATTACTGTGC TGATGGTTAT 
TACGGTGACC CTACTGGCAT CCAAGGCCAG CGTACAGCCT GCGGACAGTG CTACTGTAAC 
TACAACATTG ACCCCAATTC TGTCGGCAAC TGTGATAGGT TCACTGGAGA ATGTCTCAAG 
TGTGTCTTTG ACACAGGAGG ATTCTATTGT GAGAACTGTT TACCTGGTTT CTATGGCAAC 
GCACTTGGTA GTCCCAAGGG AGAGCAGTGT CAGGCCTGCA ACTGCTACGT CAGTGGCTCG 
ACTAGTAACG AATGTGACCA ATCCAATGGC CAGTGTAGCT GCATGCCTTT CGTGACCGGC 
CGTGACTGCA GCGAATGCGA AGCCGGCTAC TGGAACATAG ACAGTGGGCG TGGCTGCGAG 
CCTTGCTACT GTGAAAGCAC CGGGTCAACG TCTGACCAGT GCGACATCCG CAGCGGTCAG 
TGTCCTTGCA AGCCGGGAGT TGATGGTCTA AGGTGTGACC AGTGCCAGTA TGACCATTAC 
GACTACTCTG TAGAAGGGTG TACTCCATGT GAATGCAATC CCCAAGGATC CCTGTACGCC 
AATTGCACAG ACGATGGTGT GTGCGACTGC CAGCCAGGAG TCATCGGTGA CAAGTGTGAT 
TCCTGTGAAG AGAACTTCTA CAACATTGCC CTGGGCTGCA TACCCTGCCC CGAGTGCTAC 
AATCTGGTAA GGGATAACGT GAACGTGCAT CGCGACAACC TGGATGCCCT GAGGGACAAG 
ATCAACCAGA TCTCCAGCAA CCCCACCGTC ATCAACGATA CAGACTTTGA GATGCGACTG 
AGGGAGCTCG AAGGACTCCT AAATCAGACC ATTGACGACG CTCAGAGAGT GACAGGTGGA 
ACCCTCGATA TCTCCTTTGT AATTTCAGAT GTTGCCGAAG CGATGGCTAT CATCAATCGT 
CTCAAGCGTA TGTTGCCTTC TGCACAGGAA TACCTTGACA ATGAGGGTTG GGCGCTGTTG 
GAGAAGCTGA GGGGACTCAC AGCTAACATG TCTGCTCAAG ATAGGCTGAT GCAAGAAATA 
GCCCGAGAGG CCAAGCAGCT AGCTGATAAT CACACTGAGA TGGCATTGAT GGTGGAGGAG 
ACCTCAGCGA GAGCTCTGGA GATTGCCTAC CTGGCCTTGG ATGCTGCACG TGACAGTGGA 
GATGGTGGGG ACCTGGAGGC TATGGTAGAA GGAGTGGAGC GCAAATATGT CGACGCCCAA 
GAACTGTTCA ACCAGGCTTC GCAAGCCGCT GACGAATGCT TTGAGAGGGC GCAACGGGCT 
GCAGAAGAGG CACTCCGCCT TCTCACCAAG GCCAACATGC CCCTCTCGCC GATTGATGTC 
AATTCTCTTC TTGATGCAGC CAATGATATA AGCTTCAGAG CAAGGCAGCT GATGGATGAA 
GCCAACGCTC TGAGGAGAGA GAACGCTGAT CTCCTAGCCC AGGTGGCGGA GGACGCCAGG 
ACAGCCGCTG ATCTGCTGGA CCAAGCCGTC GCAGCGGAGA ACTTGATTGA TCTGTTGCTG 
GCCAAGGCCG ATAATGCTCG TTCCATTGCC TTGGAAGCCA AGAGGAAAGG AGATGAGACA 
CTTCAAGAAG CCAAAGACAT CCTCAGGGTA TTACAAGGAT TCAACGAGGA GATTGAAGAG 
AGCAAGCAGG CAGCTGACGA TGCCAAGCTC AGAATCCCTG ACATCGAGGC TATGATCAAC 
CAGGCCAACA TCACAGCCCA CAATGCATCA GATGCCATCG CAGCTGCAGA GGGCGATGCC 
ATGAAGGCAC GAGACTACGC CAGGATGGCA GAAGGAAACG CAACTTTAGC TTCAGAGGCT 
GCCAGGGGGG TCAGGACAGA CGCCGAGGCC ACCATGCTTG AGGCACAGGA ACAGAACGAC 
CGCGCTGACG ACCTCGCTGC TGATGCAATG GAAACCATGA TGAGACTCAA TGCCATCGAG 
GGCCAGGCAG AAGACGATGG GGATATGGCA GATCGGGCTC AGGAGAAGGC GAACGCTGCC 
AAACAGGTGA CGGGTGCTGC CTTACAGGCT GCCGAGGAAG CTCTAGCTCA ACTCCAGATG 
ATAAAAGGAG AGCTAGATAG TGCTGGTTCT ATCGACCTTG AGAGACTACA GGACCTGGAG 
GATGAACTCC TCAACGTCAA CGCCACCATA ACCTATGACC TCAGGCTAGA CGATACCATG 
GATGAACTAC GGGCCTACGC CATGCAGCAA GCAGGCTGGA TCTCAGCGTA TCAATTTGAC 
CTCAGTGAAA TACGAGCAGA GGTCGCATCG CTGGAAACTG TCCGGGATTC CTTGCCTAAT 
TTCTGTCCCA ACATTGTTCG GCTTGAGGAA GCGCCCGCAG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011670786.1
CDS	1..4368
Translation	MGKSFEITYIRLKFnullSPRPESFAIYKRTCQDCPWIPYQYYSGSCRGTYTLPDSEYVSYDDETLALCTSEFSDISPLTGGNIAFSTLENRPSARSFDNSAVLQDWVTATDILVMLTRLNTFGDEVFRVPNVLRSYFFAITDFSIGGRCKCNGHASHCVEAPNDPYNRLVCQCEHNTAGPDCGECLPLYNDRKWTRATAGNANECMRCNCNFHADRCFFDEELYNRTGSGGHCMECRDETSGVSCERCRDNYFRTSPDARCQPCNCDQTGSLNQQCDSSGRCQCKVGVGGDKCNQCLPNFYDFGEGGCRSCECVRAGSVDNNPDCNQGTGLCNCKLHVEGRDCETCKPGFFNLQEADEFGCLPCFCHGHSSSCSSAPGYDIAYIESNFNSGVDGWKAESRNGDQELLVYNAIRQDIGIATVDNQPMYFIAPDEYLGNKLFSYGQELSFKLRLGSDDVSLSVEDLVLEGGGRQISAPLVAQGNPRPGVYPQKFTFKLRAEDDMGWVQRPSAFEFQSLLSNLTAIKIRATYSDRGQGLLDDVRLVSGRRSVGPQSTRATFVELCSCPTGYVGQFCESCAAGYRRDPYGNGPFTRCIPCECNGHADYCDVDTGECICNHNTMGTSCELCQDGFYGNALEGTAYDCTRCPCPGGTNCFVDNLGDVVCTECPSGYVGNRCDYCADGYYGDPTGIQGQRTACGQCYCNYNIDPNSVGNCDRFTGECLKCVFDTGGFYCENCLPGFYGNALGSPKGEQCQACNCYVSGSTSNECDQSNGQCSCMPFVTGRDCSECEAGYWNIDSGRGCEPCYCESTGSTSDQCDIRSGQCPCKPGVDGLRCDQCQYDHYDYSVEGCTPCECNPQGSLYANCTDDGVCDCQPGVIGDKCDSCEENFYNIALGCIPCPECYNLVRDNVNVHRDNLDALRDKINQISSNPTVINDTDFEMRLRELEGLLNQTIDDAQRVTGGTLDISFVISDVAEAMAIINRLKRMLPSAQEYLDNEGWALLEKLRGLTANMSAQDRLMQEIAREAKQLADNHTEMALMVEETSARALEIAYLALDAARDSGDGGDLEAMVEGVERKYVDAQELFNQASQAADECFERAQRAAEEALRLLTKANMPLSPIDVNSLLDAANDISFRARQLMDEANALRRENADLLAQVAEDARTAADLLDQAVAAENLIDLLLAKADNARSIALEAKRKGDETLQEAKDILRVLQGFNEEIEESKQAADDAKLRIPDIEAMINQANITAHNASDAIAAAEGDAMKARDYARMAEGNATLASEAARGVRTDAEATMLEAQEQNDRADDLAADAMETMMRLNAIEGQAEDDGDMADRAQEKANAAKQVTGAALQAAEEALAQLQMIKGELDSAGSIDLERLQDLEDELLNVNATITYDLRLDDTMDELRAYAMQQAGWISAYQFDLSEIRAEVASLETVRDSLPNFCPNIVRLEEAPADP

Target ORF information:

RefSeq Version	XM_011672484.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus laminin subunit gamma-1 (LOC587252), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011672484.1

ORF Insert Sequence:

ATGGGTAAAT CCTTTGAGAT CACATACATT CGGCTGAAAT TTNACTCGCC GCGGCCGGAG 
AGTTTTGCCA TCTACAAGCG TACGTGTCAG GACTGTCCTT GGATACCCTA CCAGTATTAC 
AGCGGCTCAT GTAGAGGAAC CTACACCCTC CCAGATAGCG AATACGTCAG CTACGATGAT 
GAAACATTGG CGCTCTGCAC CAGTGAATTC AGCGACATCT CGCCACTCAC AGGAGGAAAT 
ATTGCTTTCA GTACGCTGGA AAATAGACCG AGTGCAAGGA GTTTTGATAA TAGTGCCGTT 
TTACAGGATT GGGTGACAGC TACAGATATT CTGGTTATGC TGACGAGATT AAACACTTTT 
GGAGATGAAG TTTTCAGGGT ACCAAACGTT CTACGATCAT ACTTCTTTGC CATCACAGAT 
TTCTCTATTG GTGGAAGGTG TAAATGCAAC GGTCACGCCA GCCACTGCGT GGAAGCACCC 
AACGATCCTT ACAACCGTCT TGTATGTCAG TGCGAACACA ACACAGCAGG GCCAGACTGT 
GGCGAATGTC TCCCTCTGTA CAATGACAGG AAGTGGACGA GAGCCACTGC TGGGAACGCA 
AATGAATGCA TGAGATGTAA CTGCAACTTC CACGCTGACC GTTGCTTCTT CGATGAAGAG 
CTTTACAACC GAACAGGATC CGGTGGTCAC TGCATGGAGT GCCGTGATGA AACTTCTGGC 
GTCAGCTGTG AGCGTTGTCG CGACAACTAC TTCAGGACTT CTCCAGACGC CCGATGCCAG 
CCTTGTAACT GTGACCAAAC AGGATCATTG AACCAGCAGT GTGATAGCTC AGGACGATGC 
CAGTGTAAGG TGGGAGTAGG AGGAGATAAA TGTAACCAGT GTCTTCCTAA CTTCTATGAC 
TTTGGTGAAG GTGGATGCAG GAGCTGCGAG TGTGTGCGTG CAGGAAGTGT AGACAACAAC 
CCAGACTGTA ACCAGGGTAC AGGTCTCTGC AACTGTAAAC TTCATGTTGA AGGAAGGGAC 
TGCGAGACCT GCAAGCCTGG TTTCTTCAAC CTTCAAGAGG CAGATGAGTT TGGATGCCTT 
CCCTGCTTCT GTCATGGTCA TTCATCCTCT TGCTCCTCTG CTCCTGGCTA TGACATTGCC 
TACATCGAGT CCAACTTCAA TTCAGGTGTG GATGGTTGGA AAGCCGAGAG TCGAAATGGA 
GACCAGGAAT TGCTTGTTTA TAATGCCATC AGGCAGGACA TCGGTATCGC AACAGTTGAC 
AACCAACCCA TGTACTTTAT AGCCCCAGAT GAGTACCTTG GTAACAAGTT GTTCAGCTAT 
GGTCAGGAGC TGAGCTTCAA GCTGAGACTG GGCAGTGATG ATGTGAGTCT CAGCGTGGAG 
GATCTCGTCT TGGAGGGAGG TGGCAGGCAG ATCTCTGCTC CGCTGGTTGC CCAGGGCAAC 
CCGCGACCAG GTGTCTACCC GCAAAAATTT ACCTTCAAAC TGCGAGCTGA GGATGATATG 
GGATGGGTTC AACGACCTTC AGCCTTCGAG TTCCAGAGCC TCCTTTCCAA CCTGACTGCC 
ATCAAGATCA GAGCAACCTA CAGTGATAGA GGTCAAGGAC TGCTTGATGA CGTCCGTCTG 
GTCAGTGGCC GGCGGTCAGT CGGTCCACAA AGCACCAGGG CTACATTCGT AGAGCTGTGC 
AGCTGCCCAA CCGGCTATGT CGGTCAATTC TGTGAGTCCT GCGCTGCTGG CTACCGCAGG 
GACCCCTACG GCAATGGACC TTTCACACGT TGCATCCCCT GCGAGTGCAA TGGACATGCC 
GACTACTGTG ATGTTGATAC TGGTGAATGT ATCTGTAACC ACAACACCAT GGGAACAAGC 
TGTGAACTAT GCCAGGATGG TTTCTATGGT AACGCCCTTG AGGGCACGGC CTATGACTGC 
ACACGCTGCC CCTGCCCCGG TGGAACCAAC TGCTTTGTCG ATAACCTAGG TGATGTGGTC 
TGTACCGAGT GCCCATCAGG CTATGTTGGA AACCGCTGTG ATTACTGTGC TGATGGTTAT 
TACGGTGACC CTACTGGCAT CCAAGGCCAG CGTACAGCCT GCGGACAGTG CTACTGTAAC 
TACAACATTG ACCCCAATTC TGTCGGCAAC TGTGATAGGT TCACTGGAGA ATGTCTCAAG 
TGTGTCTTTG ACACAGGAGG ATTCTATTGT GAGAACTGTT TACCTGGTTT CTATGGCAAC 
GCACTTGGTA GTCCCAAGGG AGAGCAGTGT CAGGCCTGCA ACTGCTACGT CAGTGGCTCG 
ACTAGTAACG AATGTGACCA ATCCAATGGC CAGTGTAGCT GCATGCCTTT CGTGACCGGC 
CGTGACTGCA GCGAATGCGA AGCCGGCTAC TGGAACATAG ACAGTGGGCG TGGCTGCGAG 
CCTTGCTACT GTGAAAGCAC CGGGTCAACG TCTGACCAGT GCGACATCCG CAGCGGTCAG 
TGTCCTTGCA AGCCGGGAGT TGATGGTCTA AGGTGTGACC AGTGCCAGTA TGACCATTAC 
GACTACTCTG TAGAAGGGTG TACTCCATGT GAATGCAATC CCCAAGGATC CCTGTACGCC 
AATTGCACAG ACGATGGTGT GTGCGACTGC CAGCCAGGAG TCATCGGTGA CAAGTGTGAT 
TCCTGTGAAG AGAACTTCTA CAACATTGCC CTGGGCTGCA TACCCTGCCC CGAGTGCTAC 
AATCTGGTAA GGGATAACGT GAACGTGCAT CGCGACAACC TGGATGCCCT GAGGGACAAG 
ATCAACCAGA TCTCCAGCAA CCCCACCGTC ATCAACGATA CAGACTTTGA GATGCGACTG 
AGGGAGCTCG AAGGACTCCT AAATCAGACC ATTGACGACG CTCAGAGAGT GACAGGTGGA 
ACCCTCGATA TCTCCTTTGT AATTTCAGAT GTTGCCGAAG CGATGGCTAT CATCAATCGT 
CTCAAGCGTA TGTTGCCTTC TGCACAGGAA TACCTTGACA ATGAGGGTTG GGCGCTGTTG 
GAGAAGCTGA GGGGACTCAC AGCTAACATG TCTGCTCAAG ATAGGCTGAT GCAAGAAATA 
GCCCGAGAGG CCAAGCAGCT AGCTGATAAT CACACTGAGA TGGCATTGAT GGTGGAGGAG 
ACCTCAGCGA GAGCTCTGGA GATTGCCTAC CTGGCCTTGG ATGCTGCACG TGACAGTGGA 
GATGGTGGGG ACCTGGAGGC TATGGTAGAA GGAGTGGAGC GCAAATATGT CGACGCCCAA 
GAACTGTTCA ACCAGGCTTC GCAAGCCGCT GACGAATGCT TTGAGAGGGC GCAACGGGCT 
GCAGAAGAGG CACTCCGCCT TCTCACCAAG GCCAACATGC CCCTCTCGCC GATTGATGTC 
AATTCTCTTC TTGATGCAGC CAATGATATA AGCTTCAGAG CAAGGCAGCT GATGGATGAA 
GCCAACGCTC TGAGGAGAGA GAACGCTGAT CTCCTAGCCC AGGTGGCGGA GGACGCCAGG 
ACAGCCGCTG ATCTGCTGGA CCAAGCCGTC GCAGCGGAGA ACTTGATTGA TCTGTTGCTG 
GCCAAGGCCG ATAATGCTCG TTCCATTGCC TTGGAAGCCA AGAGGAAAGG AGATGAGACA 
CTTCAAGAAG CCAAAGACAT CCTCAGGGTA TTACAAGGAT TCAACGAGGA GATTGAAGAG 
AGCAAGCAGG CAGCTGACGA TGCCAAGCTC AGAATCCCTG ACATCGAGGC TATGATCAAC 
CAGGCCAACA TCACAGCCCA CAATGCATCA GATGCCATCG CAGCTGCAGA GGGCGATGCC 
ATGAAGGCAC GAGACTACGC CAGGATGGCA GAAGGAAACG CAACTTTAGC TTCAGAGGCT 
GCCAGGGGGG TCAGGACAGA CGCCGAGGCC ACCATGCTTG AGGCACAGGA ACAGAACGAC 
CGCGCTGACG ACCTCGCTGC TGATGCAATG GAAACCATGA TGAGACTCAA TGCCATCGAG 
GGCCAGGCAG AAGACGATGG GGATATGGCA GATCGGGCTC AGGAGAAGGC GAACGCTGCC 
AAACAGGTGA CGGGTGCTGC CTTACAGGCT GCCGAGGAAG CTCTAGCTCA ACTCCAGATG 
ATAAAAGGAG AGCTAGATAG TGCTGGTTCT ATCGACCTTG AGAGACTACA GGACCTGGAG 
GATGAACTCC TCAACGTCAA CGCCACCATA ACCTATGACC TCAGGCTAGA CGATACCATG 
GATGAACTAC GGGCCTACGC CATGCAGCAA GCAGGCTGGA TCTCAGCGTA TCAATTTGAC 
CTCAGTGAAA TACGAGCAGA GGTCGCATCG CTGGAAACTG TCCGGGATTC CTTGCCTAAT 
TTCTGTCCCA ACATTGTTCG GCTTGAGGAA GCGCCCGCAG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC587252 ( XM_030997903.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030853763.1 Strongylocentrotus purpuratus laminin subunit gamma-1 (LOC587252), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd63704
Clone ID Related Accession (Same CDS sequence)	XM_030997903.1
Accession Version	XM_030997903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	laminin subunit gamma-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145610.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTC TCCTAGTTCT TGGTATGGTG GCACTCTGTC GTGCCAACCA GAATTCTATA 
CCTTGCTATG ACGATCTATC CAGACCTCAA AGATGCGTTC CCGAGTTCGA AAATGCAGCT 
TTCAACTTGA GCGTAGAGGC GACGAATACC TGCGGAGAAT ACCGCGACAC CCGGTACTGT 
AGGCAGACAG GCGTGCAGGG GGGAGCCCGG GGCCGTGGGC AGTGCTATAA CTGCCAGAGC 
GGCTCGCATC CCGCTCGATA CCTTACGGAT TTTCACAGTG AAGAAAGGAT GACGAGATGG 
CAGAGTGAAA CGATGTATGA AGATATTCAG TATCCAAACT CAGTCAATCT CACCATACAT 
TTAGGTAAAT CCTTTGAGAT CACATACATT CGGCTGAAAT TTTACTCGCC GCGGCCGGAG 
AGTTTTGCCA TCTACAAGCG TACGTGTCAG GACTGTCCTT GGATACCCTA CCAGTATTAC 
AGCGGCTCAT GTAGAGGAAC CTACAACCTC CCAGATAGCG AATACGTCAG CTACGATGAT 
GAAACATTGG CGCTCTGCAC CAGCGAATTC AGCGACATCT CGCCACTCAC GGGAGGAAAT 
ATTGCTTTCA GTACGCTGGA AAATAGACCG AGTGCAAGGA GTTTTGATAA TAGTGCCGTT 
TTACAGGATT GGGTGACAGC TACAGATATT CTGGTTATGC TGACGAGATT AAACACTTTT 
GGAGATGAAG TTTTCAGGGT ACCAAACGTT CTACGATCAT ACTTCTTTGC CATCACAGAT 
TTCTCTATTG GTGGAAGGTG TAAATGCAAC GGTCACGCCA GCCACTGCGT GGAAGCACCC 
AACGATCCTT ACAACCGTCT TGTATGTCAG TGCGAACACA ACACAGCAGG GCCAGACTGT 
GGCGAATGTC TCCCTCTGTA CAATGACAGG AAGTGGACGA GAGCCACTGC TGGGAACGCA 
AATGAATGCA TGAGATGTAA CTGCAACTTC CACGCTGACC GTTGTTTCTT CGATGAAGAG 
CTTTACAACC GAACAGGATC CGGTGGTCAC TGCATGGAGT GCCGCGATGA AACTTCTGGC 
GTCAGCTGTG AGCGTTGTCG CGACAACTAC TTCAGGACTT CTCCAGATGC CCGATGCCAG 
CCTTGTAACT GTGACCAAAC AGGATCATTG AACCAGCAGT GTGATAGCTC AGGACGATGC 
CAGTGTAAGG TGGGAGTAGG AGGAGATAAA TGTAACCAGT GTCTTCCTAA CTTCTATGAC 
TTTGGTGAAG GTGGATGCAG GAGCTGCGAG TGTGTGCGTG CAGGAAGTGT AGACAACAAC 
CCAGACTGTA ACCAGGGTAC AGGTCTCTGC AACTGTAAAC TTCATGTTGA AGGAAGGGAC 
TGCGAGACCT GCAAGCCTGG TTTCTTCAAC CTTCAAGAGG CGGATGAGTT TGGATGCCTT 
CCCTGCTTCT GTCATGGTCA TTCATCCTCT TGCTCCTCTG CTCCTGGCTA TGACATTGCC 
TACATCGAGT CCAACTTCAA TTCAGGTGTG GATGGTTGGA AAGCCGAGAG TCGAAATGGA 
GACCAGGAAT TGCTTGTTTA TAATGCCATC AGGCAGGACA TCGGTATCGC AACAGTTGAC 
AACCAACCCA TGTACTTTAT AGCCCCAGAT GAGTACCTTG GTAGCAAGTT GTTCAGCTAT 
GGTCAGGAGC TGAGCTTCAA GCTGAGACTG GGCAGTGATG ATGTGAGTCT CAGCGTGGAG 
GATCTCGTCT TGGAGGGAGG TGGCAGGCAG ATCTCTGCTC CGCTAGTTGC CCAGGGCAAC 
CCGCGACCAG GTGTCTACCC GCAAAAATTT ACCTTCAAAC TGCGAGCTGA GGATGACATG 
GGATGGGTTC AACGACCTTC AGCCTTTGAG TTCCAGAGCC TCCTTTCCAA CCTGACTGCC 
ATCAAGATCA GAGCAACCTA CAGTGATAGA GGTCAAGGGC TGCTTGATGA CGTCCGTCTG 
GTCAGTGGCC GGCGGTCAGT CGGACCACAA AGCACCAGGG CTACATTCGT GGAGCTGTGC 
AGCTGCCCAA CCGGCTATGT CGGTCAATTC TGTGAGTCCT GCGCTGCTGG CTACCGCAGG 
GACCCCTACG GCAACGGACC TTTCACACGT TGCATCCCCT GCGAATGCAA TGGACATGCC 
GACTACTGTG ATGTCGATAC TGGTGAATGT ATCTGTAACC ACAACACCAT GGGTACAAGC 
TGTGAACTAT GCCAGGATGG TTTCTATGGT AACGCCCTTG AGGGCACGGC CTATGACTGC 
ACGCGCTGCC CCTGCCCCGG TGGAACCAAC TGCTTTGTCG ATAACCTAGG TGATGTGGTC 
TGTACCGAGT GCCCATCAGG CTATGTTGGA AACCGCTGTG ATTACTGTGC TGATGGTTAT 
TACGGTGACC CCACTGGCAT CCAGGGTCAG CGAACGGCCT GCGGACAGTG CTACTGTAAC 
TACAACATTG ACCCCAATTC TGTCGGCAAC TGTGATAGGT TCACTGGAGA ATGTCTCAAG 
TGTGTCTTTG ACACAGGAGG ATTCTATTGT GAGAACTGTT TACCTGGTTT CTATGGCAAC 
GCACTTGGTA GTCCCAAGGG AGAGCAGTGT CAGGCCTGCA ACTGCTACGT GAGTGGCTCG 
ACTAGTAACG AATGTGACCA ATCCAATGGC CAGTGTAGCT GCATGCCTTT CGTGACCGGC 
CGTGACTGCA GCGAATGCGA AGCCGGCTAC TGGAACATCG ACAGTGGACG TGGCTGCGAG 
CCTTGCTACT GTGAAAGCAC CGGGTCAACG TCTGACCAGT GCGACATCCG CAGCGGTCAG 
TGTCCTTGCA AGCCAGGAGT GGATGGTCTA AGGTGTGACC AGTGCCAGTA TGACCATTAT 
GACTACTCTG TAGAAGGATG TACTCCATGT GAATGCAATC CCCAAGGATC CCTGTACGCT 
AATTGCACAG ACGACGGTGT GTGCGACTGC CAGCCAGGAG TCATCGGTGA CAAGTGTGAT 
TCCTGTGAGG AGAACTTCTA CAACATTGCC CTGGGCTGCA TACCCTGCCC CGAGTGCTAC 
AATCTGGTAA GGGATAACGT GAACGTGCAT CGCGACAACC TGGATGCCCT GAGGGACAAG 
ATCAACCAGA TCTCCAGCAA CCCCACCGTC ATCAACGATA CAGACTTTGA GATGCGACTG 
AGGGAGCTCG AAGGACTCCT AAATCAGACC ATTGACGACG CTCAGAGAGT GACAGCTGAG 
GACCGTACCT TAGCCGATAA TCTGAACGCC ATTAAGGATG ATCTAGGAGA CCTCAGACTA 
GCCTTAGCTC GTATCAGGGC GAAGATCGAC CAGGCAGATG GAGTTACAGA GGCTGCCAAA 
CAAGATGTTG CCGAAGCGAT GGCTATCATC AATCGTCTCA AGCGTATGTT GCCTTCTGCA 
CAGGAATACC TTGACAATGA GGGTTGGGCG CTGTTGGAGA AGCTGAGGGG ACTCACAGCT 
AACATGTCTG CTCAAGATAG GCTGATGCAA GAAATAGCCC GAGAGGCCAA GCAGCTAGCT 
GATAATCACA CTGAGATGGC ATTGATGGTG GAGGAGACCT CAGCGAGAGC TCTGGAGATT 
GCCTACCTGG CCTTGGATGC TGCACGTGAC AGTGGAGATG GTGGGGACCT GGAGGCAATG 
GTAGAAGGAG TGGAGCGCAA ATATGTCGAC GCCCAAGAAC TGTTCAACCA GGCTTCGCAA 
GCCGCTGACG AATGCTTTGA GAGGGCGCAA CGGGCTGCAG AAGAGGCACT CCGCCTTCTC 
ACCAAGGCCA ACATGCCCCT CTCGCCGATT GATGTCAATT CTCTTCTTGA TGCAGCCAAT 
GATATAAGCT TCAGAGCAAG GCAGCTGATG GATGAAGCCA ACGCTCTGAG GAGAGAGAAC 
GCTGATCTCC TAGCCCAGGT GGCGGAGGAC GCCAGGACAG CCGCTGATCT GCTGGACCAA 
GCCGTCGCAG CGGAGAACTT GATTGATCTG TTGCTGGCCA AGGCCGATAA TGCTCGTTCC 
ATTGCCTTGG AAGCCAAGAG GAAAGGAGAT GAGACACTTC AAGAAGCCAA AGACATCCTC 
AGGGTATTAC AAGGATTCAA CGAGGAGATT GAAGAGAGCA AGCAGGCAGC TGACGATGCC 
AAGCTCAGAA TCCCTGACAT CGAGGCTATG ATCAACCAGG CCAACATCAC AGCCCACAAT 
GCATCAGATG CCATCGCAGC TGCAGAGGGC GATGCCATGA AGGCACGAGA CTACGCCAGG 
ATGGCAGAAG GAAACGCAAC TTTAGCTTCA GAGGCTGCCA GGGGGGTCAG GACAGACGCC 
GAGGCCACCA TGCTTGAGGC ACAGGAACAG AACGACCGCG CTGACGACCT CGCTGCTGAT 
GCAATGGAAA CCATGATGAG ACTCAATGCC ATCGAGGGCC AGGCAGAAGA CGATGGGGAT 
ATGGCAGATC GGGCTCAGGA GAAGGCGAAC GCTGCCAAAC AGGTGACGGG TGCTGCCTTA 
CAGGCTGCCG AGGAAGCTCT AGCTCAACTC CAGATGATAA AAGGAGAGCT AGATAGTGCT 
GGTTCTATCG ACCTTGAGAG ACTACAGGAC CTGGAGGATG AACTCCTCAA CGTCAACGCC 
ACCATAACCT ATGACCTCAG GCTAGACGAT ACCATGGATG AACTACGGGC CTACGCCATG 
CAGCAAGCAG GCTGGATCTC AGCGTATCAA TTTGACCTCA GTGAAATACG AGCAGAGGTG 
GCCAATGTGG AAGAAATCAG GGATGCCTTG CCTGAGTTCT GCCCCAACGA AAGCATCCTA 
GAACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030853763.1
CDS	201..5009
Translation	MKFLLVLGMVALCRANQNSIPCYDDLSRPQRCVPEFENAAFNLSVEATNTCGEYRDTRYCRQTGVQGGARGRGQCYNCQSGSHPARYLTDFHSEERMTRWQSETMYEDIQYPNSVNLTIHLGKSFEITYIRLKFYSPRPESFAIYKRTCQDCPWIPYQYYSGSCRGTYNLPDSEYVSYDDETLALCTSEFSDISPLTGGNIAFSTLENRPSARSFDNSAVLQDWVTATDILVMLTRLNTFGDEVFRVPNVLRSYFFAITDFSIGGRCKCNGHASHCVEAPNDPYNRLVCQCEHNTAGPDCGECLPLYNDRKWTRATAGNANECMRCNCNFHADRCFFDEELYNRTGSGGHCMECRDETSGVSCERCRDNYFRTSPDARCQPCNCDQTGSLNQQCDSSGRCQCKVGVGGDKCNQCLPNFYDFGEGGCRSCECVRAGSVDNNPDCNQGTGLCNCKLHVEGRDCETCKPGFFNLQEADEFGCLPCFCHGHSSSCSSAPGYDIAYIESNFNSGVDGWKAESRNGDQELLVYNAIRQDIGIATVDNQPMYFIAPDEYLGSKLFSYGQELSFKLRLGSDDVSLSVEDLVLEGGGRQISAPLVAQGNPRPGVYPQKFTFKLRAEDDMGWVQRPSAFEFQSLLSNLTAIKIRATYSDRGQGLLDDVRLVSGRRSVGPQSTRATFVELCSCPTGYVGQFCESCAAGYRRDPYGNGPFTRCIPCECNGHADYCDVDTGECICNHNTMGTSCELCQDGFYGNALEGTAYDCTRCPCPGGTNCFVDNLGDVVCTECPSGYVGNRCDYCADGYYGDPTGIQGQRTACGQCYCNYNIDPNSVGNCDRFTGECLKCVFDTGGFYCENCLPGFYGNALGSPKGEQCQACNCYVSGSTSNECDQSNGQCSCMPFVTGRDCSECEAGYWNIDSGRGCEPCYCESTGSTSDQCDIRSGQCPCKPGVDGLRCDQCQYDHYDYSVEGCTPCECNPQGSLYANCTDDGVCDCQPGVIGDKCDSCEENFYNIALGCIPCPECYNLVRDNVNVHRDNLDALRDKINQISSNPTVINDTDFEMRLRELEGLLNQTIDDAQRVTAEDRTLADNLNAIKDDLGDLRLALARIRAKIDQADGVTEAAKQDVAEAMAIINRLKRMLPSAQEYLDNEGWALLEKLRGLTANMSAQDRLMQEIAREAKQLADNHTEMALMVEETSARALEIAYLALDAARDSGDGGDLEAMVEGVERKYVDAQELFNQASQAADECFERAQRAAEEALRLLTKANMPLSPIDVNSLLDAANDISFRARQLMDEANALRRENADLLAQVAEDARTAADLLDQAVAAENLIDLLLAKADNARSIALEAKRKGDETLQEAKDILRVLQGFNEEIEESKQAADDAKLRIPDIEAMINQANITAHNASDAIAAAEGDAMKARDYARMAEGNATLASEAARGVRTDAEATMLEAQEQNDRADDLAADAMETMMRLNAIEGQAEDDGDMADRAQEKANAAKQVTGAALQAAEEALAQLQMIKGELDSAGSIDLERLQDLEDELLNVNATITYDLRLDDTMDELRAYAMQQAGWISAYQFDLSEIRAEVANVEEIRDALPEFCPNESILEP

Target ORF information:

RefSeq Version	XM_030997903.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus laminin subunit gamma-1 (LOC587252), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030997903.1

ORF Insert Sequence:

ATGAAGTTTC TCCTAGTTCT TGGTATGGTG GCACTCTGTC GTGCCAACCA GAATTCTATA 
CCTTGCTATG ACGATCTATC CAGACCTCAA AGATGCGTTC CCGAGTTCGA AAATGCAGCT 
TTCAACTTGA GCGTAGAGGC GACGAATACC TGCGGAGAAT ACCGCGACAC CCGGTACTGT 
AGGCAGACAG GCGTGCAGGG GGGAGCCCGG GGCCGTGGGC AGTGCTATAA CTGCCAGAGC 
GGCTCGCATC CCGCTCGATA CCTTACGGAT TTTCACAGTG AAGAAAGGAT GACGAGATGG 
CAGAGTGAAA CGATGTATGA AGATATTCAG TATCCAAACT CAGTCAATCT CACCATACAT 
TTAGGTAAAT CCTTTGAGAT CACATACATT CGGCTGAAAT TTTACTCGCC GCGGCCGGAG 
AGTTTTGCCA TCTACAAGCG TACGTGTCAG GACTGTCCTT GGATACCCTA CCAGTATTAC 
AGCGGCTCAT GTAGAGGAAC CTACAACCTC CCAGATAGCG AATACGTCAG CTACGATGAT 
GAAACATTGG CGCTCTGCAC CAGCGAATTC AGCGACATCT CGCCACTCAC GGGAGGAAAT 
ATTGCTTTCA GTACGCTGGA AAATAGACCG AGTGCAAGGA GTTTTGATAA TAGTGCCGTT 
TTACAGGATT GGGTGACAGC TACAGATATT CTGGTTATGC TGACGAGATT AAACACTTTT 
GGAGATGAAG TTTTCAGGGT ACCAAACGTT CTACGATCAT ACTTCTTTGC CATCACAGAT 
TTCTCTATTG GTGGAAGGTG TAAATGCAAC GGTCACGCCA GCCACTGCGT GGAAGCACCC 
AACGATCCTT ACAACCGTCT TGTATGTCAG TGCGAACACA ACACAGCAGG GCCAGACTGT 
GGCGAATGTC TCCCTCTGTA CAATGACAGG AAGTGGACGA GAGCCACTGC TGGGAACGCA 
AATGAATGCA TGAGATGTAA CTGCAACTTC CACGCTGACC GTTGTTTCTT CGATGAAGAG 
CTTTACAACC GAACAGGATC CGGTGGTCAC TGCATGGAGT GCCGCGATGA AACTTCTGGC 
GTCAGCTGTG AGCGTTGTCG CGACAACTAC TTCAGGACTT CTCCAGATGC CCGATGCCAG 
CCTTGTAACT GTGACCAAAC AGGATCATTG AACCAGCAGT GTGATAGCTC AGGACGATGC 
CAGTGTAAGG TGGGAGTAGG AGGAGATAAA TGTAACCAGT GTCTTCCTAA CTTCTATGAC 
TTTGGTGAAG GTGGATGCAG GAGCTGCGAG TGTGTGCGTG CAGGAAGTGT AGACAACAAC 
CCAGACTGTA ACCAGGGTAC AGGTCTCTGC AACTGTAAAC TTCATGTTGA AGGAAGGGAC 
TGCGAGACCT GCAAGCCTGG TTTCTTCAAC CTTCAAGAGG CGGATGAGTT TGGATGCCTT 
CCCTGCTTCT GTCATGGTCA TTCATCCTCT TGCTCCTCTG CTCCTGGCTA TGACATTGCC 
TACATCGAGT CCAACTTCAA TTCAGGTGTG GATGGTTGGA AAGCCGAGAG TCGAAATGGA 
GACCAGGAAT TGCTTGTTTA TAATGCCATC AGGCAGGACA TCGGTATCGC AACAGTTGAC 
AACCAACCCA TGTACTTTAT AGCCCCAGAT GAGTACCTTG GTAGCAAGTT GTTCAGCTAT 
GGTCAGGAGC TGAGCTTCAA GCTGAGACTG GGCAGTGATG ATGTGAGTCT CAGCGTGGAG 
GATCTCGTCT TGGAGGGAGG TGGCAGGCAG ATCTCTGCTC CGCTAGTTGC CCAGGGCAAC 
CCGCGACCAG GTGTCTACCC GCAAAAATTT ACCTTCAAAC TGCGAGCTGA GGATGACATG 
GGATGGGTTC AACGACCTTC AGCCTTTGAG TTCCAGAGCC TCCTTTCCAA CCTGACTGCC 
ATCAAGATCA GAGCAACCTA CAGTGATAGA GGTCAAGGGC TGCTTGATGA CGTCCGTCTG 
GTCAGTGGCC GGCGGTCAGT CGGACCACAA AGCACCAGGG CTACATTCGT GGAGCTGTGC 
AGCTGCCCAA CCGGCTATGT CGGTCAATTC TGTGAGTCCT GCGCTGCTGG CTACCGCAGG 
GACCCCTACG GCAACGGACC TTTCACACGT TGCATCCCCT GCGAATGCAA TGGACATGCC 
GACTACTGTG ATGTCGATAC TGGTGAATGT ATCTGTAACC ACAACACCAT GGGTACAAGC 
TGTGAACTAT GCCAGGATGG TTTCTATGGT AACGCCCTTG AGGGCACGGC CTATGACTGC 
ACGCGCTGCC CCTGCCCCGG TGGAACCAAC TGCTTTGTCG ATAACCTAGG TGATGTGGTC 
TGTACCGAGT GCCCATCAGG CTATGTTGGA AACCGCTGTG ATTACTGTGC TGATGGTTAT 
TACGGTGACC CCACTGGCAT CCAGGGTCAG CGAACGGCCT GCGGACAGTG CTACTGTAAC 
TACAACATTG ACCCCAATTC TGTCGGCAAC TGTGATAGGT TCACTGGAGA ATGTCTCAAG 
TGTGTCTTTG ACACAGGAGG ATTCTATTGT GAGAACTGTT TACCTGGTTT CTATGGCAAC 
GCACTTGGTA GTCCCAAGGG AGAGCAGTGT CAGGCCTGCA ACTGCTACGT GAGTGGCTCG 
ACTAGTAACG AATGTGACCA ATCCAATGGC CAGTGTAGCT GCATGCCTTT CGTGACCGGC 
CGTGACTGCA GCGAATGCGA AGCCGGCTAC TGGAACATCG ACAGTGGACG TGGCTGCGAG 
CCTTGCTACT GTGAAAGCAC CGGGTCAACG TCTGACCAGT GCGACATCCG CAGCGGTCAG 
TGTCCTTGCA AGCCAGGAGT GGATGGTCTA AGGTGTGACC AGTGCCAGTA TGACCATTAT 
GACTACTCTG TAGAAGGATG TACTCCATGT GAATGCAATC CCCAAGGATC CCTGTACGCT 
AATTGCACAG ACGACGGTGT GTGCGACTGC CAGCCAGGAG TCATCGGTGA CAAGTGTGAT 
TCCTGTGAGG AGAACTTCTA CAACATTGCC CTGGGCTGCA TACCCTGCCC CGAGTGCTAC 
AATCTGGTAA GGGATAACGT GAACGTGCAT CGCGACAACC TGGATGCCCT GAGGGACAAG 
ATCAACCAGA TCTCCAGCAA CCCCACCGTC ATCAACGATA CAGACTTTGA GATGCGACTG 
AGGGAGCTCG AAGGACTCCT AAATCAGACC ATTGACGACG CTCAGAGAGT GACAGCTGAG 
GACCGTACCT TAGCCGATAA TCTGAACGCC ATTAAGGATG ATCTAGGAGA CCTCAGACTA 
GCCTTAGCTC GTATCAGGGC GAAGATCGAC CAGGCAGATG GAGTTACAGA GGCTGCCAAA 
CAAGATGTTG CCGAAGCGAT GGCTATCATC AATCGTCTCA AGCGTATGTT GCCTTCTGCA 
CAGGAATACC TTGACAATGA GGGTTGGGCG CTGTTGGAGA AGCTGAGGGG ACTCACAGCT 
AACATGTCTG CTCAAGATAG GCTGATGCAA GAAATAGCCC GAGAGGCCAA GCAGCTAGCT 
GATAATCACA CTGAGATGGC ATTGATGGTG GAGGAGACCT CAGCGAGAGC TCTGGAGATT 
GCCTACCTGG CCTTGGATGC TGCACGTGAC AGTGGAGATG GTGGGGACCT GGAGGCAATG 
GTAGAAGGAG TGGAGCGCAA ATATGTCGAC GCCCAAGAAC TGTTCAACCA GGCTTCGCAA 
GCCGCTGACG AATGCTTTGA GAGGGCGCAA CGGGCTGCAG AAGAGGCACT CCGCCTTCTC 
ACCAAGGCCA ACATGCCCCT CTCGCCGATT GATGTCAATT CTCTTCTTGA TGCAGCCAAT 
GATATAAGCT TCAGAGCAAG GCAGCTGATG GATGAAGCCA ACGCTCTGAG GAGAGAGAAC 
GCTGATCTCC TAGCCCAGGT GGCGGAGGAC GCCAGGACAG CCGCTGATCT GCTGGACCAA 
GCCGTCGCAG CGGAGAACTT GATTGATCTG TTGCTGGCCA AGGCCGATAA TGCTCGTTCC 
ATTGCCTTGG AAGCCAAGAG GAAAGGAGAT GAGACACTTC AAGAAGCCAA AGACATCCTC 
AGGGTATTAC AAGGATTCAA CGAGGAGATT GAAGAGAGCA AGCAGGCAGC TGACGATGCC 
AAGCTCAGAA TCCCTGACAT CGAGGCTATG ATCAACCAGG CCAACATCAC AGCCCACAAT 
GCATCAGATG CCATCGCAGC TGCAGAGGGC GATGCCATGA AGGCACGAGA CTACGCCAGG 
ATGGCAGAAG GAAACGCAAC TTTAGCTTCA GAGGCTGCCA GGGGGGTCAG GACAGACGCC 
GAGGCCACCA TGCTTGAGGC ACAGGAACAG AACGACCGCG CTGACGACCT CGCTGCTGAT 
GCAATGGAAA CCATGATGAG ACTCAATGCC ATCGAGGGCC AGGCAGAAGA CGATGGGGAT 
ATGGCAGATC GGGCTCAGGA GAAGGCGAAC GCTGCCAAAC AGGTGACGGG TGCTGCCTTA 
CAGGCTGCCG AGGAAGCTCT AGCTCAACTC CAGATGATAA AAGGAGAGCT AGATAGTGCT 
GGTTCTATCG ACCTTGAGAG ACTACAGGAC CTGGAGGATG AACTCCTCAA CGTCAACGCC 
ACCATAACCT ATGACCTCAG GCTAGACGAT ACCATGGATG AACTACGGGC CTACGCCATG 
CAGCAAGCAG GCTGGATCTC AGCGTATCAA TTTGACCTCA GTGAAATACG AGCAGAGGTG 
GCCAATGTGG AAGAAATCAG GGATGCCTTG CCTGAGTTCT GCCCCAACGA AAGCATCCTA 
GAACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC587252 ( XM_030997904.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030853764.1 Strongylocentrotus purpuratus laminin subunit gamma-1 (LOC587252), transcript variant X2, mRNA.

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CloneID	OSd63705
Clone ID Related Accession (Same CDS sequence)	XM_030997904.1
Accession Version	XM_030997904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	laminin subunit gamma-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145610.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTC TCCTAGTTCT TGGTATGGTG GCACTCTGTC GTGCCAACCA GAATTCTATA 
CCTTGCTATG ACGATCTATC CAGACCTCAA AGATGCGTTC CCGAGTTCGA AAATGCAGCT 
TTCAACTTGA GCGTAGAGGC GACGAATACC TGCGGAGAAT ACCGCGACAC CCGGTACTGT 
AGGCAGACAG GCGTGCAGGG GGGAGCCCGG GGCCGTGGGC AGTGCTATAA CTGCCAGAGC 
GGCTCGCATC CCGCTCGATA CCTTACGGAT TTTCACAGTG AAGAAAGGAT GACGAGATGG 
CAGAGTGAAA CGATGTATGA AGATATTCAG TATCCAAACT CAGTCAATCT CACCATACAT 
TTAGGTAAAT CCTTTGAGAT CACATACATT CGGCTGAAAT TTTACTCGCC GCGGCCGGAG 
AGTTTTGCCA TCTACAAGCG TACGTGTCAG GACTGTCCTT GGATACCCTA CCAGTATTAC 
AGCGGCTCAT GTAGAGGAAC CTACAACCTC CCAGATAGCG AATACGTCAG CTACGATGAT 
GAAACATTGG CGCTCTGCAC CAGCGAATTC AGCGACATCT CGCCACTCAC GGGAGGAAAT 
ATTGCTTTCA GTACGCTGGA AAATAGACCG AGTGCAAGGA GTTTTGATAA TAGTGCCGTT 
TTACAGGATT GGGTGACAGC TACAGATATT CTGGTTATGC TGACGAGATT AAACACTTTT 
GGAGATGAAG TTTTCAGGGT ACCAAACGTT CTACGATCAT ACTTCTTTGC CATCACAGAT 
TTCTCTATTG GTGGAAGGTG TAAATGCAAC GGTCACGCCA GCCACTGCGT GGAAGCACCC 
AACGATCCTT ACAACCGTCT TGTATGTCAG TGCGAACACA ACACAGCAGG GCCAGACTGT 
GGCGAATGTC TCCCTCTGTA CAATGACAGG AAGTGGACGA GAGCCACTGC TGGGAACGCA 
AATGAATGCA TGAGATGTAA CTGCAACTTC CACGCTGACC GTTGTTTCTT CGATGAAGAG 
CTTTACAACC GAACAGGATC CGGTGGTCAC TGCATGGAGT GCCGCGATGA AACTTCTGGC 
GTCAGCTGTG AGCGTTGTCG CGACAACTAC TTCAGGACTT CTCCAGATGC CCGATGCCAG 
CCTTGTAACT GTGACCAAAC AGGATCATTG AACCAGCAGT GTGATAGCTC AGGACGATGC 
CAGTGTAAGG TGGGAGTAGG AGGAGATAAA TGTAACCAGT GTCTTCCTAA CTTCTATGAC 
TTTGGTGAAG GTGGATGCAG GAGCTGCGAG TGTGTGCGTG CAGGAAGTGT AGACAACAAC 
CCAGACTGTA ACCAGGGTAC AGGTCTCTGC AACTGTAAAC TTCATGTTGA AGGAAGGGAC 
TGCGAGACCT GCAAGCCTGG TTTCTTCAAC CTTCAAGAGG CGGATGAGTT TGGATGCCTT 
CCCTGCTTCT GTCATGGTCA TTCATCCTCT TGCTCCTCTG CTCCTGGCTA TGACATTGCC 
TACATCGAGT CCAACTTCAA TTCAGGTGTG GATGGTTGGA AAGCCGAGAG TCGAAATGGA 
GACCAGGAAT TGCTTGTTTA TAATGCCATC AGGCAGGACA TCGGTATCGC AACAGTTGAC 
AACCAACCCA TGTACTTTAT AGCCCCAGAT GAGTACCTTG GTAGCAAGTT GTTCAGCTAT 
GGTCAGGAGC TGAGCTTCAA GCTGAGACTG GGCAGTGATG ATGTGAGTCT CAGCGTGGAG 
GATCTCGTCT TGGAGGGAGG TGGCAGGCAG ATCTCTGCTC CGCTAGTTGC CCAGGGCAAC 
CCGCGACCAG GTGTCTACCC GCAAAAATTT ACCTTCAAAC TGCGAGCTGA GGATGACATG 
GGATGGGTTC AACGACCTTC AGCCTTTGAG TTCCAGAGCC TCCTTTCCAA CCTGACTGCC 
ATCAAGATCA GAGCAACCTA CAGTGATAGA GGTCAAGGGC TGCTTGATGA CGTCCGTCTG 
GTCAGTGGCC GGCGGTCAGT CGGACCACAA AGCACCAGGG CTACATTCGT GGAGCTGTGC 
AGCTGCCCAA CCGGCTATGT CGGTCAATTC TGTGAGTCCT GCGCTGCTGG CTACCGCAGG 
GACCCCTACG GCAACGGACC TTTCACACGT TGCATCCCCT GCGAATGCAA TGGACATGCC 
GACTACTGTG ATGTCGATAC TGGTGAATGT ATCTGTAACC ACAACACCAT GGGTACAAGC 
TGTGAACTAT GCCAGGATGG TTTCTATGGT AACGCCCTTG AGGGCACGGC CTATGACTGC 
ACGCGCTGCC CCTGCCCCGG TGGAACCAAC TGCTTTGTCG ATAACCTAGG TGATGTGGTC 
TGTACCGAGT GCCCATCAGG CTATGTTGGA AACCGCTGTG ATTACTGTGC TGATGGTTAT 
TACGGTGACC CCACTGGCAT CCAGGGTCAG CGAACGGCCT GCGGACAGTG CTACTGTAAC 
TACAACATTG ACCCCAATTC TGTCGGCAAC TGTGATAGGT TCACTGGAGA ATGTCTCAAG 
TGTGTCTTTG ACACAGGAGG ATTCTATTGT GAGAACTGTT TACCTGGTTT CTATGGCAAC 
GCACTTGGTA GTCCCAAGGG AGAGCAGTGT CAGGCCTGCA ACTGCTACGT GAGTGGCTCG 
ACTAGTAACG AATGTGACCA ATCCAATGGC CAGTGTAGCT GCATGCCTTT CGTGACCGGC 
CGTGACTGCA GCGAATGCGA AGCCGGCTAC TGGAACATCG ACAGTGGACG TGGCTGCGAG 
CCTTGCTACT GTGAAAGCAC CGGGTCAACG TCTGACCAGT GCGACATCCG CAGCGGTCAG 
TGTCCTTGCA AGCCAGGAGT GGATGGTCTA AGGTGTGACC AGTGCCAGTA TGACCATTAT 
GACTACTCTG TAGAAGGATG TACTCCATGT GAATGCAATC CCCAAGGATC CCTGTACGCT 
AATTGCACAG ACGACGGTGT GTGCGACTGC CAGCCAGGAG TCATCGGTGA CAAGTGTGAT 
TCCTGTGAGG AGAACTTCTA CAACATTGCC CTGGGCTGCA TACCCTGCCC CGAGTGCTAC 
AATCTGGTAA GGGATAACGT GAACGTGCAT CGCGACAACC TGGATGCCCT GAGGGACAAG 
ATCAACCAGA TCTCCAGCAA CCCCACCGTC ATCAACGATA CAGACTTTGA GATGCGACTG 
AGGGAGCTCG AAGGACTCCT AAATCAGACC ATTGACGACG CTCAGAGAGT GACAGCTGAG 
GACCGTACCT TAGCCGATAA TCTGAACGCC ATTAAGGATG ATCTAGGAGA CCTCAGACTA 
GCCTTAGCTC GTATCAGGGC GAAGATCGAC CAGGCAGATG GAGTTACAGA GGCTGCCAAA 
CAAGATGTTG CCGAAGCGAT GGCTATCATC AATCGTCTCA AGCGTATGTT GCCTTCTGCA 
CAGGAATACC TTGACAATGA GGGTTGGGCG CTGTTGGAGA AGCTGAGGGG ACTCACAGCT 
AACATGTCTG CTCAAGATAG GCTGATGCAA GAAATAGCCC GAGAGGCCAA GCAGCTAGCT 
GATAATCACA CTGAGATGGC ATTGATGGTG GAGGAGACCT CAGCGAGAGC TCTGGAGATT 
GCCTACCTGG CCTTGGATGC TGCACGTGAC AGTGGAGATG GTGGGGACCT GGAGGCAATG 
GTAGAAGGAG TGGAGCGCAA ATATGTCGAC GCCCAAGAAC TGTTCAACCA GGCTTCGCAA 
GCCGCTGACG AATGCTTTGA GAGGGCGCAA CGGGCTGCAG AAGAGGCACT CCGCCTTCTC 
ACCAAGGCCA ACATGCCCCT CTCGCCGATT GATGTCAATT CTCTTCTTGA TGCAGCCAAT 
GATATAAGCT TCAGAGCAAG GCAGCTGATG GATGAAGCCA ACGCTCTGAG GAGAGAGAAC 
GCTGATCTCC TAGCCCAGGT GGCGGAGGAC GCCAGGACAG CCGCTGATCT GCTGGACCAA 
GCCGTCGCAG CGGAGAACTT GATTGATCTG TTGCTGGCCA AGGCCGATAA TGCTCGTTCC 
ATTGCCTTGG AAGCCAAGAG GAAAGGAGAT GAGACACTTC AAGAAGCCAA AGACATCCTC 
AGGGTATTAC AAGGATTCAA CGAGGAGATT GAAGAGAGCA AGCAGGCAGC TGACGATGCC 
AAGCTCAGAA TCCCTGACAT CGAGGCTATG ATCAACCAGG CCAACATCAC AGCCCACAAT 
GCATCAGATG CCATCGCAGC TGCAGAGGGC GATGCCATGA AGGCACGAGA CTACGCCAGG 
ATGGCAGAAG GAAACGCAAC TTTAGCTTCA GAGGCTGCCA GGGGGGTCAG GACAGACGCC 
GAGGCCACCA TGCTTGAGGC ACAGGAACAG AACGACCGCG CTGACGACCT CGCTGCTGAT 
GCAATGGAAA CCATGATGAG ACTCAATGCC ATCGAGGGCC AGGCAGAAGA CGATGGGGAT 
ATGGCAGATC GGGCTCAGGA GAAGGCGAAC GCTGCCAAAC AGGTGACGGG TGCTGCCTTA 
CAGGCTGCCG AGGAAGCTCT AGCTCAACTC CAGATGATAA AAGGAGAGCT AGATAGTGCT 
GGTTCTATAG GCCTGATTGA CACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030853764.1
CDS	202..4788
Translation	MKFLLVLGMVALCRANQNSIPCYDDLSRPQRCVPEFENAAFNLSVEATNTCGEYRDTRYCRQTGVQGGARGRGQCYNCQSGSHPARYLTDFHSEERMTRWQSETMYEDIQYPNSVNLTIHLGKSFEITYIRLKFYSPRPESFAIYKRTCQDCPWIPYQYYSGSCRGTYNLPDSEYVSYDDETLALCTSEFSDISPLTGGNIAFSTLENRPSARSFDNSAVLQDWVTATDILVMLTRLNTFGDEVFRVPNVLRSYFFAITDFSIGGRCKCNGHASHCVEAPNDPYNRLVCQCEHNTAGPDCGECLPLYNDRKWTRATAGNANECMRCNCNFHADRCFFDEELYNRTGSGGHCMECRDETSGVSCERCRDNYFRTSPDARCQPCNCDQTGSLNQQCDSSGRCQCKVGVGGDKCNQCLPNFYDFGEGGCRSCECVRAGSVDNNPDCNQGTGLCNCKLHVEGRDCETCKPGFFNLQEADEFGCLPCFCHGHSSSCSSAPGYDIAYIESNFNSGVDGWKAESRNGDQELLVYNAIRQDIGIATVDNQPMYFIAPDEYLGSKLFSYGQELSFKLRLGSDDVSLSVEDLVLEGGGRQISAPLVAQGNPRPGVYPQKFTFKLRAEDDMGWVQRPSAFEFQSLLSNLTAIKIRATYSDRGQGLLDDVRLVSGRRSVGPQSTRATFVELCSCPTGYVGQFCESCAAGYRRDPYGNGPFTRCIPCECNGHADYCDVDTGECICNHNTMGTSCELCQDGFYGNALEGTAYDCTRCPCPGGTNCFVDNLGDVVCTECPSGYVGNRCDYCADGYYGDPTGIQGQRTACGQCYCNYNIDPNSVGNCDRFTGECLKCVFDTGGFYCENCLPGFYGNALGSPKGEQCQACNCYVSGSTSNECDQSNGQCSCMPFVTGRDCSECEAGYWNIDSGRGCEPCYCESTGSTSDQCDIRSGQCPCKPGVDGLRCDQCQYDHYDYSVEGCTPCECNPQGSLYANCTDDGVCDCQPGVIGDKCDSCEENFYNIALGCIPCPECYNLVRDNVNVHRDNLDALRDKINQISSNPTVINDTDFEMRLRELEGLLNQTIDDAQRVTAEDRTLADNLNAIKDDLGDLRLALARIRAKIDQADGVTEAAKQDVAEAMAIINRLKRMLPSAQEYLDNEGWALLEKLRGLTANMSAQDRLMQEIAREAKQLADNHTEMALMVEETSARALEIAYLALDAARDSGDGGDLEAMVEGVERKYVDAQELFNQASQAADECFERAQRAAEEALRLLTKANMPLSPIDVNSLLDAANDISFRARQLMDEANALRRENADLLAQVAEDARTAADLLDQAVAAENLIDLLLAKADNARSIALEAKRKGDETLQEAKDILRVLQGFNEEIEESKQAADDAKLRIPDIEAMINQANITAHNASDAIAAAEGDAMKARDYARMAEGNATLASEAARGVRTDAEATMLEAQEQNDRADDLAADAMETMMRLNAIEGQAEDDGDMADRAQEKANAAKQVTGAALQAAEEALAQLQMIKGELDSAGSIGLIDT

Target ORF information:

RefSeq Version	XM_030997904.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus laminin subunit gamma-1 (LOC587252), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030997904.1

ORF Insert Sequence:

ATGAAGTTTC TCCTAGTTCT TGGTATGGTG GCACTCTGTC GTGCCAACCA GAATTCTATA 
CCTTGCTATG ACGATCTATC CAGACCTCAA AGATGCGTTC CCGAGTTCGA AAATGCAGCT 
TTCAACTTGA GCGTAGAGGC GACGAATACC TGCGGAGAAT ACCGCGACAC CCGGTACTGT 
AGGCAGACAG GCGTGCAGGG GGGAGCCCGG GGCCGTGGGC AGTGCTATAA CTGCCAGAGC 
GGCTCGCATC CCGCTCGATA CCTTACGGAT TTTCACAGTG AAGAAAGGAT GACGAGATGG 
CAGAGTGAAA CGATGTATGA AGATATTCAG TATCCAAACT CAGTCAATCT CACCATACAT 
TTAGGTAAAT CCTTTGAGAT CACATACATT CGGCTGAAAT TTTACTCGCC GCGGCCGGAG 
AGTTTTGCCA TCTACAAGCG TACGTGTCAG GACTGTCCTT GGATACCCTA CCAGTATTAC 
AGCGGCTCAT GTAGAGGAAC CTACAACCTC CCAGATAGCG AATACGTCAG CTACGATGAT 
GAAACATTGG CGCTCTGCAC CAGCGAATTC AGCGACATCT CGCCACTCAC GGGAGGAAAT 
ATTGCTTTCA GTACGCTGGA AAATAGACCG AGTGCAAGGA GTTTTGATAA TAGTGCCGTT 
TTACAGGATT GGGTGACAGC TACAGATATT CTGGTTATGC TGACGAGATT AAACACTTTT 
GGAGATGAAG TTTTCAGGGT ACCAAACGTT CTACGATCAT ACTTCTTTGC CATCACAGAT 
TTCTCTATTG GTGGAAGGTG TAAATGCAAC GGTCACGCCA GCCACTGCGT GGAAGCACCC 
AACGATCCTT ACAACCGTCT TGTATGTCAG TGCGAACACA ACACAGCAGG GCCAGACTGT 
GGCGAATGTC TCCCTCTGTA CAATGACAGG AAGTGGACGA GAGCCACTGC TGGGAACGCA 
AATGAATGCA TGAGATGTAA CTGCAACTTC CACGCTGACC GTTGTTTCTT CGATGAAGAG 
CTTTACAACC GAACAGGATC CGGTGGTCAC TGCATGGAGT GCCGCGATGA AACTTCTGGC 
GTCAGCTGTG AGCGTTGTCG CGACAACTAC TTCAGGACTT CTCCAGATGC CCGATGCCAG 
CCTTGTAACT GTGACCAAAC AGGATCATTG AACCAGCAGT GTGATAGCTC AGGACGATGC 
CAGTGTAAGG TGGGAGTAGG AGGAGATAAA TGTAACCAGT GTCTTCCTAA CTTCTATGAC 
TTTGGTGAAG GTGGATGCAG GAGCTGCGAG TGTGTGCGTG CAGGAAGTGT AGACAACAAC 
CCAGACTGTA ACCAGGGTAC AGGTCTCTGC AACTGTAAAC TTCATGTTGA AGGAAGGGAC 
TGCGAGACCT GCAAGCCTGG TTTCTTCAAC CTTCAAGAGG CGGATGAGTT TGGATGCCTT 
CCCTGCTTCT GTCATGGTCA TTCATCCTCT TGCTCCTCTG CTCCTGGCTA TGACATTGCC 
TACATCGAGT CCAACTTCAA TTCAGGTGTG GATGGTTGGA AAGCCGAGAG TCGAAATGGA 
GACCAGGAAT TGCTTGTTTA TAATGCCATC AGGCAGGACA TCGGTATCGC AACAGTTGAC 
AACCAACCCA TGTACTTTAT AGCCCCAGAT GAGTACCTTG GTAGCAAGTT GTTCAGCTAT 
GGTCAGGAGC TGAGCTTCAA GCTGAGACTG GGCAGTGATG ATGTGAGTCT CAGCGTGGAG 
GATCTCGTCT TGGAGGGAGG TGGCAGGCAG ATCTCTGCTC CGCTAGTTGC CCAGGGCAAC 
CCGCGACCAG GTGTCTACCC GCAAAAATTT ACCTTCAAAC TGCGAGCTGA GGATGACATG 
GGATGGGTTC AACGACCTTC AGCCTTTGAG TTCCAGAGCC TCCTTTCCAA CCTGACTGCC 
ATCAAGATCA GAGCAACCTA CAGTGATAGA GGTCAAGGGC TGCTTGATGA CGTCCGTCTG 
GTCAGTGGCC GGCGGTCAGT CGGACCACAA AGCACCAGGG CTACATTCGT GGAGCTGTGC 
AGCTGCCCAA CCGGCTATGT CGGTCAATTC TGTGAGTCCT GCGCTGCTGG CTACCGCAGG 
GACCCCTACG GCAACGGACC TTTCACACGT TGCATCCCCT GCGAATGCAA TGGACATGCC 
GACTACTGTG ATGTCGATAC TGGTGAATGT ATCTGTAACC ACAACACCAT GGGTACAAGC 
TGTGAACTAT GCCAGGATGG TTTCTATGGT AACGCCCTTG AGGGCACGGC CTATGACTGC 
ACGCGCTGCC CCTGCCCCGG TGGAACCAAC TGCTTTGTCG ATAACCTAGG TGATGTGGTC 
TGTACCGAGT GCCCATCAGG CTATGTTGGA AACCGCTGTG ATTACTGTGC TGATGGTTAT 
TACGGTGACC CCACTGGCAT CCAGGGTCAG CGAACGGCCT GCGGACAGTG CTACTGTAAC 
TACAACATTG ACCCCAATTC TGTCGGCAAC TGTGATAGGT TCACTGGAGA ATGTCTCAAG 
TGTGTCTTTG ACACAGGAGG ATTCTATTGT GAGAACTGTT TACCTGGTTT CTATGGCAAC 
GCACTTGGTA GTCCCAAGGG AGAGCAGTGT CAGGCCTGCA ACTGCTACGT GAGTGGCTCG 
ACTAGTAACG AATGTGACCA ATCCAATGGC CAGTGTAGCT GCATGCCTTT CGTGACCGGC 
CGTGACTGCA GCGAATGCGA AGCCGGCTAC TGGAACATCG ACAGTGGACG TGGCTGCGAG 
CCTTGCTACT GTGAAAGCAC CGGGTCAACG TCTGACCAGT GCGACATCCG CAGCGGTCAG 
TGTCCTTGCA AGCCAGGAGT GGATGGTCTA AGGTGTGACC AGTGCCAGTA TGACCATTAT 
GACTACTCTG TAGAAGGATG TACTCCATGT GAATGCAATC CCCAAGGATC CCTGTACGCT 
AATTGCACAG ACGACGGTGT GTGCGACTGC CAGCCAGGAG TCATCGGTGA CAAGTGTGAT 
TCCTGTGAGG AGAACTTCTA CAACATTGCC CTGGGCTGCA TACCCTGCCC CGAGTGCTAC 
AATCTGGTAA GGGATAACGT GAACGTGCAT CGCGACAACC TGGATGCCCT GAGGGACAAG 
ATCAACCAGA TCTCCAGCAA CCCCACCGTC ATCAACGATA CAGACTTTGA GATGCGACTG 
AGGGAGCTCG AAGGACTCCT AAATCAGACC ATTGACGACG CTCAGAGAGT GACAGCTGAG 
GACCGTACCT TAGCCGATAA TCTGAACGCC ATTAAGGATG ATCTAGGAGA CCTCAGACTA 
GCCTTAGCTC GTATCAGGGC GAAGATCGAC CAGGCAGATG GAGTTACAGA GGCTGCCAAA 
CAAGATGTTG CCGAAGCGAT GGCTATCATC AATCGTCTCA AGCGTATGTT GCCTTCTGCA 
CAGGAATACC TTGACAATGA GGGTTGGGCG CTGTTGGAGA AGCTGAGGGG ACTCACAGCT 
AACATGTCTG CTCAAGATAG GCTGATGCAA GAAATAGCCC GAGAGGCCAA GCAGCTAGCT 
GATAATCACA CTGAGATGGC ATTGATGGTG GAGGAGACCT CAGCGAGAGC TCTGGAGATT 
GCCTACCTGG CCTTGGATGC TGCACGTGAC AGTGGAGATG GTGGGGACCT GGAGGCAATG 
GTAGAAGGAG TGGAGCGCAA ATATGTCGAC GCCCAAGAAC TGTTCAACCA GGCTTCGCAA 
GCCGCTGACG AATGCTTTGA GAGGGCGCAA CGGGCTGCAG AAGAGGCACT CCGCCTTCTC 
ACCAAGGCCA ACATGCCCCT CTCGCCGATT GATGTCAATT CTCTTCTTGA TGCAGCCAAT 
GATATAAGCT TCAGAGCAAG GCAGCTGATG GATGAAGCCA ACGCTCTGAG GAGAGAGAAC 
GCTGATCTCC TAGCCCAGGT GGCGGAGGAC GCCAGGACAG CCGCTGATCT GCTGGACCAA 
GCCGTCGCAG CGGAGAACTT GATTGATCTG TTGCTGGCCA AGGCCGATAA TGCTCGTTCC 
ATTGCCTTGG AAGCCAAGAG GAAAGGAGAT GAGACACTTC AAGAAGCCAA AGACATCCTC 
AGGGTATTAC AAGGATTCAA CGAGGAGATT GAAGAGAGCA AGCAGGCAGC TGACGATGCC 
AAGCTCAGAA TCCCTGACAT CGAGGCTATG ATCAACCAGG CCAACATCAC AGCCCACAAT 
GCATCAGATG CCATCGCAGC TGCAGAGGGC GATGCCATGA AGGCACGAGA CTACGCCAGG 
ATGGCAGAAG GAAACGCAAC TTTAGCTTCA GAGGCTGCCA GGGGGGTCAG GACAGACGCC 
GAGGCCACCA TGCTTGAGGC ACAGGAACAG AACGACCGCG CTGACGACCT CGCTGCTGAT 
GCAATGGAAA CCATGATGAG ACTCAATGCC ATCGAGGGCC AGGCAGAAGA CGATGGGGAT 
ATGGCAGATC GGGCTCAGGA GAAGGCGAAC GCTGCCAAAC AGGTGACGGG TGCTGCCTTA 
CAGGCTGCCG AGGAAGCTCT AGCTCAACTC CAGATGATAA AAGGAGAGCT AGATAGTGCT 
GGTTCTATAG GCCTGATTGA CACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC587252 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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