LOC586153 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC586153
Entrez Gene ID	586153
Full Name	host cell factor 2
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011683701.1	XP_011682003.1	host cell factor 1 isoform X2
XM_031000420.1	XP_030856280.1	host cell factor 1 isoform X2
XM_011683700.1	XP_011682002.1	host cell factor 2 isoform X1
XM_031000419.1	XP_030856279.1	host cell factor isoform X1
XM_785946.4	XP_791039.4	host cell factor 2 isoform X3
XM_031000423.1	XP_030856283.1	host cell factor 1 isoform X4
XM_011683702.1	XP_011682004.1	host cell factor 1 isoform X4
XM_031000422.1	XP_030856282.1	host cell factor 2 isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC586153 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC586153 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd25224	XM_011683700.1 Latest version!	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSd25225	XM_011683701.1 Latest version!	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSd25226	XM_785946.4 Latest version!	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSd25227	XM_011683702.1 Latest version!	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSd66402	XM_031000419.1 Latest version!	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSd66403	XM_031000420.1 Latest version!	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSd66404	XM_031000422.1 Latest version!	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSd66405	XM_031000423.1 Latest version!	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC586153 ( XM_011683700.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011682002.1 Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd25224
Clone ID Related Accession (Same CDS sequence)	XM_011683700.1
Accession Version	XM_011683700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	host cell factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011994856.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCA CGCACAAACT CCCTGCTTGG TACATTGCAT TGTGGGTAAG AGACAAAATG 
GCGGCTCCCA TACTAAAATG GAAGCGTGTG ACAAATACGA CTGGTCCAAG CCCCCGACCT 
CGCCATGGAC ATCGAGCCGT CGCAATCAAA GACCTTATGG TAGTCTTCGG CGGTGGGAAT 
GAGGGGATCG TGGATGAACT TCATGTTTAC AATACAGCTA CCAACCAGTG GTTTGTCCCA 
GCAGTGAGAG GGGATATACC CCCAGGCTGT GCAGCTTATG GCTTTGTCAG TGATGGCACC 
CGTCTCTTCA TATTTGGAGG AATGGTGGAG TATGGGAAGT ACTCCAACGA GCTATATGAA 
CTGCAAGCCA GTAGATGGGA ATGGAAACGA CTCAAGCCTA AGACTGCTAA GAATGCACCA 
CCCCCATGCC CTCGACTTGG TCACACGTTC ACCATGCTTG GGAGTAAAGC ATATCTCTTT 
GGAGGACTTG CCAATGATAG TGATGATCCA AAGAATAATA TACCAAGGTA TCTCAATGAT 
CTCTACTCAC TGGAGCTGAG ATCAGGCTAC TCGAACATGA TATGGGACAT ACCCCTAACC 
ACAGGACCTG CCCCTCCACC TAGAGAATCA CATACCGTTG TAGGTTATGC TCCCAAGGAT 
GGCTCCTTTA ATCGACTCAT TGTGTATGGT GGTATGAGCG GCTGTCGGCT CGGTGACCTT 
TGGCAACTCA ACATGGATAC ACATACATGG ATCAAACCTG AACTCAATGG ACCCAACCCA 
CTACCAAGAA GCTTGCACTC TGCCACTGTC ATCGGAAATA GAATGTTTGT GTTTGGTGGC 
TGGGTACCTC TTGTGATGGA TGATGTCAAA GTAGCAGCTC ATGAAAAGGA ATGGAAGTGT 
ACAAACACAC TGGCTTCTCT CAATCTAGCA ACCCACACAT GGGAGCCACT GGCGATGGAG 
GTGTTTGAAG AGGCTGTACC TAGAGCCAGG GCTGGGCATT GCTCTGTGGC TATCCATACC 
AGACTCTACG TATGGAGTGG AAGGGACGGA TATCGCAAGG CATGGAATAA CCAGGTGTGC 
TGCAAAGACC TTTGGTTCTT GGAGACTGAG AAGCCGATGG CCCCAGGCAG GGTCCAGCTC 
GTCCGAGCTA GCACCACCAC ACTGGAAGTA TCATGGGGTG CTGTAGCCAC AGCCGATGCC 
TATTTACTAC AGCTGCAGAA GTATGACATG CCCCCTACCT CAAGTGCAGC CTTATCCTCT 
GTGGGTGGCG CTGCTTCCAC CAAGCCACTC CAGTCACCAA TCAAAGCACA AGCAACCACC 
ACGTTCCGTG TGGTGGCTCC AGGCTCCTCA GCCTCTTGCC CTATTCAGTT GATAAAACAC 
ACTCCAGGAG TTAAGATTCA GAGTCCAACC TCCATGGCTG GCTCAGCAGC CAGTTCACTC 
TTCAAACTCA GCAAGCCCCT CCCTACTCCC GGTGTCCAGC AGCAGAACAC CCTGAGCGGT 
ATCGTAGCTC TCGCTGAGGC TGCTGCAGGC ACCCAGAAGA TGGTCAGTAG TACCACCGCT 
GTCTCACCAA CCCCCAACAA ACCGCTCCTG GCAACGGTAG GAGGGTCCAA CCTGACCCTG 
GTGACCCGGT CAATGCCAGG AGGTGTCAGC GTCACATTGC CGCAAGGGAT GAAGCTCACC 
ACGGCAGCAG GAGGTGGGCT CAAGATGACA ACAGCTGGAG GCAAACCCCA ATTGGTGTCA 
GCTATGGTTT CAGGCACCAC TAAGACTGTT ACACTTGTAC AAAACGCTGG TGCTAACAAG 
GCAGGTGGTG TAGGGCCCGG CACTACCATC GTAAAACTTG TCTCAACCAC CCAGGCTGGA 
GGCAAGCCTA CTACCATCAT CACCCCTATC AGCCAGGCAG GGTTGAAAGG TCAGCCTACG 
ATCCTGGGTA TCAGCAGCAT CACGGCAGGT CAGCAAGGCA AAAGCATCAT TAAGACCATT 
CCAGTTGGTA AAGCAGGTGT CGCCAACACC GCAACCGGCA AGACGCCCAC CATCGTCACT 
GTAACCAACA AGATGCTGGC AGGAGCCACG GGTACTCCCA CCAAGTTTAT CACGACTGGA 
GGGGTTGGTA AGCAGCAGAA GATTGTCTTG ACAACCCAGG CAGGCGCCTC CCCTACCATC 
CTATCAGGAA CACTCAAAGG TCTAGGGGCA GGGAATTCCC CCATCACCAT CATCCGTGCT 
GTAGCGCCCC AGGGTTCGTC TCCCGGAGCC AGCGGTCAGG TCTCAGGTAA CACCATACAG 
ATCATTACCA AGGGTGGGGC TAAGAGTACC CTCAACCAGG CCAGTGCTGC TAACCTACTC 
AAATCACTCT CCACCTCTCA AGGCCTGACT ACGCCACTCA CAATCACCAC CACCGCTAAC 
TCATCGGGAG TCACAACTAA GCTGGTAACC ATTCCCATTT CAGCCCTGAC GGGGGCAGCA 
ACCACCAGCG TTTCCCCTGC CATAAACGCA ATCATGTCTA CAACAGCCCA GGTTGTCTCA 
AACACAGCTG CTGTGCAAAA GACCATCACT ACCCCTGTAG CATTGAGCAC TGTTGGTACG 
GCACAGACTA CTCTCATATC AACGGTAGGA ACAACACCTA CAGTCAGCCT TCCGAGTACA 
GATCAACAGA AGGTGGCTGA AGATGCATCA GAAACCAAAC CTACAGCTAC AGAAGAAAGC 
AAAGAAAACA AAGAAGCACA ATCAACAACA GACACAACGG CAACACCAAC TCCTACAACA 
CAAGAGGCTG CAACCCCTGC CACTACAGAG GCATCAGAGG GCGGTGTAAC GATAAAGAAG 
GAAGAGCAGA CTTCAGAACC AAATCAACAA CCCAAGGTAG AATCAGCTGC TTCAGAAGCC 
GCCACGCCCA TGGACACCGA CCCCTCCCCT GCACCCGGTA CAGGCATACC GGCCGAGCCT 
ACCGATACCA CCAAGACCAA TGGCTCCACC CCTGCTGAAG AGACGAAGAA CAAGGCCTTG 
GATAACGGGG CAGTGGGAGA AGAGCCGATG GATACGAAAG AGAGCAACAC AGATCCAGAT 
TCAGATCCTA AGTCTATTGC CATGACAATT GCATCACTTG CTAATTCGGC CGGTCCCCCT 
GTGATGTCGA CCAGGCCTAT CACCAATGGA CTGGACTCAA AAACGGAGGA TGGCAAGTCA 
CCGATGGGGC AAGCCATCCA GCTAGCCAAG AAAGATGTCA ACCAGTGGTA CGACGTTGGA 
ATTATTAAGA CAACAACCAC TACAGTAGCG CATTATCACT TGCCATCAGA GATCATTAGC 
AGAAATGAAG ATGACATTGA TGTTGTCAGT GTGCCAGATC ACAGCTTCCT CAAGAAGCAG 
GAGCTCCACC CGGGCACAGC CTATAAGTTC AGGGTCGCTG GTATCAATGC TAGCGGGCGG 
GGTCCATGGA GCGAGATCTC CGCATTCAAG ACATGCTTGC CTGGATTCCC AGGGGCACCT 
TCAGCTATTA GAATCAGCAA GAGTCCCGAT GGAGCTCATC TATCCTGGGA GCCTCCGGCA 
AACACCAACG GTGAGATCCA GGAGTATTCT GTCTACCTTG CCGTACGTAG CACCATGAGC 
GGGGCGTCGG GAGATGCCAA GCCCACTACC CCAGCCCAGC TAGCCTTCGT CAGGGTGTAC 
TGCGGACCCA ACCCATCTTG CGTCGTGAGC TCGGCTAACC TGGCCGCAGC TCACATCGAC 
TGCACCAGCA AACCTGCTAT CATTTTCAGA ATCGCTGCAC GTAACGTCAA GGGATATGGA 
CCAGCCACTC AGGTTAGATG GCTTCAAGAT AACCAGACTG CTGCTAAACC AACAACTACT 
GTTGCTGCCA AGAGACCAGC ATCTTCTATA GAAAGTCCGA TGGTGAAGAA AGTAAAAGCA 
GAAAACGACA GTGATTCCAA GACGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011682002.1
CDS	36..3965
Translation	MDATHKLPAWYIALWVRDKMAAPILKWKRVTNTTGPSPRPRHGHRAVAIKDLMVVFGGGNEGIVDELHVYNTATNQWFVPAVRGDIPPGCAAYGFVSDGTRLFIFGGMVEYGKYSNELYELQASRWEWKRLKPKTAKNAPPPCPRLGHTFTMLGSKAYLFGGLANDSDDPKNNIPRYLNDLYSLELRSGYSNMIWDIPLTTGPAPPPRESHTVVGYAPKDGSFNRLIVYGGMSGCRLGDLWQLNMDTHTWIKPELNGPNPLPRSLHSATVIGNRMFVFGGWVPLVMDDVKVAAHEKEWKCTNTLASLNLATHTWEPLAMEVFEEAVPRARAGHCSVAIHTRLYVWSGRDGYRKAWNNQVCCKDLWFLETEKPMAPGRVQLVRASTTTLEVSWGAVATADAYLLQLQKYDMPPTSSAALSSVGGAASTKPLQSPIKAQATTTFRVVAPGSSASCPIQLIKHTPGVKIQSPTSMAGSAASSLFKLSKPLPTPGVQQQNTLSGIVALAEAAAGTQKMVSSTTAVSPTPNKPLLATVGGSNLTLVTRSMPGGVSVTLPQGMKLTTAAGGGLKMTTAGGKPQLVSAMVSGTTKTVTLVQNAGANKAGGVGPGTTIVKLVSTTQAGGKPTTIITPISQAGLKGQPTILGISSITAGQQGKSIIKTIPVGKAGVANTATGKTPTIVTVTNKMLAGATGTPTKFITTGGVGKQQKIVLTTQAGASPTILSGTLKGLGAGNSPITIIRAVAPQGSSPGASGQVSGNTIQIITKGGAKSTLNQASAANLLKSLSTSQGLTTPLTITTTANSSGVTTKLVTIPISALTGAATTSVSPAINAIMSTTAQVVSNTAAVQKTITTPVALSTVGTAQTTLISTVGTTPTVSLPSTDQQKVAEDASETKPTATEESKENKEAQSTTDTTATPTPTTQEAATPATTEASEGGVTIKKEEQTSEPNQQPKVESAASEAATPMDTDPSPAPGTGIPAEPTDTTKTNGSTPAEETKNKALDNGAVGEEPMDTKESNTDPDSDPKSIAMTIASLANSAGPPVMSTRPITNGLDSKTEDGKSPMGQAIQLAKKDVNQWYDVGIIKTTTTTVAHYHLPSEIISRNEDDIDVVSVPDHSFLKKQELHPGTAYKFRVAGINASGRGPWSEISAFKTCLPGFPGAPSAIRISKSPDGAHLSWEPPANTNGEIQEYSVYLAVRSTMSGASGDAKPTTPAQLAFVRVYCGPNPSCVVSSANLAAAHIDCTSKPAIIFRIAARNVKGYGPATQVRWLQDNQTAAKPTTTVAAKRPASSIESPMVKKVKAENDSDSKTA

Target ORF information:

RefSeq Version	XM_011683700.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011683700.1

ORF Insert Sequence:

ATGGATGCCA CGCACAAACT CCCTGCTTGG TACATTGCAT TGTGGGTAAG AGACAAAATG 
GCGGCTCCCA TACTAAAATG GAAGCGTGTG ACAAATACGA CTGGTCCAAG CCCCCGACCT 
CGCCATGGAC ATCGAGCCGT CGCAATCAAA GACCTTATGG TAGTCTTCGG CGGTGGGAAT 
GAGGGGATCG TGGATGAACT TCATGTTTAC AATACAGCTA CCAACCAGTG GTTTGTCCCA 
GCAGTGAGAG GGGATATACC CCCAGGCTGT GCAGCTTATG GCTTTGTCAG TGATGGCACC 
CGTCTCTTCA TATTTGGAGG AATGGTGGAG TATGGGAAGT ACTCCAACGA GCTATATGAA 
CTGCAAGCCA GTAGATGGGA ATGGAAACGA CTCAAGCCTA AGACTGCTAA GAATGCACCA 
CCCCCATGCC CTCGACTTGG TCACACGTTC ACCATGCTTG GGAGTAAAGC ATATCTCTTT 
GGAGGACTTG CCAATGATAG TGATGATCCA AAGAATAATA TACCAAGGTA TCTCAATGAT 
CTCTACTCAC TGGAGCTGAG ATCAGGCTAC TCGAACATGA TATGGGACAT ACCCCTAACC 
ACAGGACCTG CCCCTCCACC TAGAGAATCA CATACCGTTG TAGGTTATGC TCCCAAGGAT 
GGCTCCTTTA ATCGACTCAT TGTGTATGGT GGTATGAGCG GCTGTCGGCT CGGTGACCTT 
TGGCAACTCA ACATGGATAC ACATACATGG ATCAAACCTG AACTCAATGG ACCCAACCCA 
CTACCAAGAA GCTTGCACTC TGCCACTGTC ATCGGAAATA GAATGTTTGT GTTTGGTGGC 
TGGGTACCTC TTGTGATGGA TGATGTCAAA GTAGCAGCTC ATGAAAAGGA ATGGAAGTGT 
ACAAACACAC TGGCTTCTCT CAATCTAGCA ACCCACACAT GGGAGCCACT GGCGATGGAG 
GTGTTTGAAG AGGCTGTACC TAGAGCCAGG GCTGGGCATT GCTCTGTGGC TATCCATACC 
AGACTCTACG TATGGAGTGG AAGGGACGGA TATCGCAAGG CATGGAATAA CCAGGTGTGC 
TGCAAAGACC TTTGGTTCTT GGAGACTGAG AAGCCGATGG CCCCAGGCAG GGTCCAGCTC 
GTCCGAGCTA GCACCACCAC ACTGGAAGTA TCATGGGGTG CTGTAGCCAC AGCCGATGCC 
TATTTACTAC AGCTGCAGAA GTATGACATG CCCCCTACCT CAAGTGCAGC CTTATCCTCT 
GTGGGTGGCG CTGCTTCCAC CAAGCCACTC CAGTCACCAA TCAAAGCACA AGCAACCACC 
ACGTTCCGTG TGGTGGCTCC AGGCTCCTCA GCCTCTTGCC CTATTCAGTT GATAAAACAC 
ACTCCAGGAG TTAAGATTCA GAGTCCAACC TCCATGGCTG GCTCAGCAGC CAGTTCACTC 
TTCAAACTCA GCAAGCCCCT CCCTACTCCC GGTGTCCAGC AGCAGAACAC CCTGAGCGGT 
ATCGTAGCTC TCGCTGAGGC TGCTGCAGGC ACCCAGAAGA TGGTCAGTAG TACCACCGCT 
GTCTCACCAA CCCCCAACAA ACCGCTCCTG GCAACGGTAG GAGGGTCCAA CCTGACCCTG 
GTGACCCGGT CAATGCCAGG AGGTGTCAGC GTCACATTGC CGCAAGGGAT GAAGCTCACC 
ACGGCAGCAG GAGGTGGGCT CAAGATGACA ACAGCTGGAG GCAAACCCCA ATTGGTGTCA 
GCTATGGTTT CAGGCACCAC TAAGACTGTT ACACTTGTAC AAAACGCTGG TGCTAACAAG 
GCAGGTGGTG TAGGGCCCGG CACTACCATC GTAAAACTTG TCTCAACCAC CCAGGCTGGA 
GGCAAGCCTA CTACCATCAT CACCCCTATC AGCCAGGCAG GGTTGAAAGG TCAGCCTACG 
ATCCTGGGTA TCAGCAGCAT CACGGCAGGT CAGCAAGGCA AAAGCATCAT TAAGACCATT 
CCAGTTGGTA AAGCAGGTGT CGCCAACACC GCAACCGGCA AGACGCCCAC CATCGTCACT 
GTAACCAACA AGATGCTGGC AGGAGCCACG GGTACTCCCA CCAAGTTTAT CACGACTGGA 
GGGGTTGGTA AGCAGCAGAA GATTGTCTTG ACAACCCAGG CAGGCGCCTC CCCTACCATC 
CTATCAGGAA CACTCAAAGG TCTAGGGGCA GGGAATTCCC CCATCACCAT CATCCGTGCT 
GTAGCGCCCC AGGGTTCGTC TCCCGGAGCC AGCGGTCAGG TCTCAGGTAA CACCATACAG 
ATCATTACCA AGGGTGGGGC TAAGAGTACC CTCAACCAGG CCAGTGCTGC TAACCTACTC 
AAATCACTCT CCACCTCTCA AGGCCTGACT ACGCCACTCA CAATCACCAC CACCGCTAAC 
TCATCGGGAG TCACAACTAA GCTGGTAACC ATTCCCATTT CAGCCCTGAC GGGGGCAGCA 
ACCACCAGCG TTTCCCCTGC CATAAACGCA ATCATGTCTA CAACAGCCCA GGTTGTCTCA 
AACACAGCTG CTGTGCAAAA GACCATCACT ACCCCTGTAG CATTGAGCAC TGTTGGTACG 
GCACAGACTA CTCTCATATC AACGGTAGGA ACAACACCTA CAGTCAGCCT TCCGAGTACA 
GATCAACAGA AGGTGGCTGA AGATGCATCA GAAACCAAAC CTACAGCTAC AGAAGAAAGC 
AAAGAAAACA AAGAAGCACA ATCAACAACA GACACAACGG CAACACCAAC TCCTACAACA 
CAAGAGGCTG CAACCCCTGC CACTACAGAG GCATCAGAGG GCGGTGTAAC GATAAAGAAG 
GAAGAGCAGA CTTCAGAACC AAATCAACAA CCCAAGGTAG AATCAGCTGC TTCAGAAGCC 
GCCACGCCCA TGGACACCGA CCCCTCCCCT GCACCCGGTA CAGGCATACC GGCCGAGCCT 
ACCGATACCA CCAAGACCAA TGGCTCCACC CCTGCTGAAG AGACGAAGAA CAAGGCCTTG 
GATAACGGGG CAGTGGGAGA AGAGCCGATG GATACGAAAG AGAGCAACAC AGATCCAGAT 
TCAGATCCTA AGTCTATTGC CATGACAATT GCATCACTTG CTAATTCGGC CGGTCCCCCT 
GTGATGTCGA CCAGGCCTAT CACCAATGGA CTGGACTCAA AAACGGAGGA TGGCAAGTCA 
CCGATGGGGC AAGCCATCCA GCTAGCCAAG AAAGATGTCA ACCAGTGGTA CGACGTTGGA 
ATTATTAAGA CAACAACCAC TACAGTAGCG CATTATCACT TGCCATCAGA GATCATTAGC 
AGAAATGAAG ATGACATTGA TGTTGTCAGT GTGCCAGATC ACAGCTTCCT CAAGAAGCAG 
GAGCTCCACC CGGGCACAGC CTATAAGTTC AGGGTCGCTG GTATCAATGC TAGCGGGCGG 
GGTCCATGGA GCGAGATCTC CGCATTCAAG ACATGCTTGC CTGGATTCCC AGGGGCACCT 
TCAGCTATTA GAATCAGCAA GAGTCCCGAT GGAGCTCATC TATCCTGGGA GCCTCCGGCA 
AACACCAACG GTGAGATCCA GGAGTATTCT GTCTACCTTG CCGTACGTAG CACCATGAGC 
GGGGCGTCGG GAGATGCCAA GCCCACTACC CCAGCCCAGC TAGCCTTCGT CAGGGTGTAC 
TGCGGACCCA ACCCATCTTG CGTCGTGAGC TCGGCTAACC TGGCCGCAGC TCACATCGAC 
TGCACCAGCA AACCTGCTAT CATTTTCAGA ATCGCTGCAC GTAACGTCAA GGGATATGGA 
CCAGCCACTC AGGTTAGATG GCTTCAAGAT AACCAGACTG CTGCTAAACC AACAACTACT 
GTTGCTGCCA AGAGACCAGC ATCTTCTATA GAAAGTCCGA TGGTGAAGAA AGTAAAAGCA 
GAAAACGACA GTGATTCCAA GACGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC586153 ( XM_011683701.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011682003.1 Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSd25225
Clone ID Related Accession (Same CDS sequence)	XM_011683701.1
Accession Version	XM_011683701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	host cell factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011994856.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCA CGCACAAACT CCCTGCTTGG TACATTGCAT TGTGGGTAAG AGACAAAATG 
GCGGCTCCCA TACTAAAATG GAAGCGTGTG ACAAATACGA CTGGTCCAAG CCCCCGACCT 
CGCCATGGAC ATCGAGCCGT CGCAATCAAA GACCTTATGG TAGTCTTCGG CGGTGGGAAT 
GAGGGGATCG TGGATGAACT TCATGTTTAC AATACAGCTA CCAACCAGTG GTTTGTCCCA 
GCAGTGAGAG GGGATATACC CCCAGGCTGT GCAGCTTATG GCTTTGTCAG TGATGGCACC 
CGTCTCTTCA TATTTGGAGG AATGGTGGAG TATGGGAAGT ACTCCAACGA GCTATATGAA 
CTGCAAGCCA GTAGATGGGA ATGGAAACGA CTCAAGCCTA AGACTGCTAA GAATGCACCA 
CCCCCATGCC CTCGACTTGG TCACACGTTC ACCATGCTTG GGAGTAAAGC ATATCTCTTT 
GGAGGACTTG CCAATGATAG TGATGATCCA AAGAATAATA TACCAAGGTA TCTCAATGAT 
CTCTACTCAC TGGAGCTGAG ATCAGGCTAC TCGAACATGA TATGGGACAT ACCCCTAACC 
ACAGGACCTG CCCCTCCACC TAGAGAATCA CATACCGTTG TAGGTTATGC TCCCAAGGAT 
GGCTCCTTTA ATCGACTCAT TGTGTATGGT GGTATGAGCG GCTGTCGGCT CGGTGACCTT 
TGGCAACTCA ACATGGATAC ACATACATGG ATCAAACCTG AACTCAATGG ACCCAACCCA 
CTACCAAGAA GCTTGCACTC TGCCACTGTC ATCGGAAATA GAATGTTTGT GTTTGGTGGC 
TGGGTACCTC TTGTGATGGA TGATGTCAAA GTAGCAGCTC ATGAAAAGGA ATGGAAGTGT 
ACAAACACAC TGGCTTCTCT CAATCTAGCA ACCCACACAT GGGAGCCACT GGCGATGGAG 
GTGTTTGAAG AGGCTGTACC TAGAGCCAGG GCTGGGCATT GCTCTGTGGC TATCCATACC 
AGACTCTACG TATGGAGTGG AAGGGACGGA TATCGCAAGG CATGGAATAA CCAGGTGTGC 
TGCAAAGACC TTTGGTTCTT GGAGACTGAG AAGCCGATGG CCCCAGGCAG GGTCCAGCTC 
GTCCGAGCTA GCACCACCAC ACTGGAAGTA TCATGGGGTG CTGTAGCCAC AGCCGATGCC 
TATTTACTAC AGCTGCAGAA GTATGACATG CCCCCTACCT CAAGTGCAGC CTTATCCTCT 
GTGGGTGGCG CTGCTTCCAC CAAGCCACTC CAGTCACCAA TCAAAGCACA AGCAACCACC 
ACGTTCCGTG TGGTGGCTCC AGGCTCCTCA GCCTCTTGCC CTATTCAGTT GATAAAACAC 
ACTCCAGGAG TTAAGATTCA GAGTCCAACC TCCATGGCTG GCTCAGCAGC CAGTTCACTC 
TTCAAACTCA GCAAGCCCCT CCCTACTCCC GGTGTCCAGC AGCAGAACAC CCTGAGCGGT 
ATCGTAGCTC TCGCTGAGGC TGCTGCAGGC ACCCAGAAGA TGGTCAGTAG TACCACCGCT 
GTCTCACCAA CCCCCAACAA ACCGCTCCTG GCAACGGTAG GAGGGTCCAA CCTGACCCTG 
GTGACCCGGT CAATGCCAGG AGGTGTCAGC GTCACATTGC CGCAAGGGAT GAAGCTCACC 
ACGGCAGCAG GAGGTGGGCT CAAGATGACA ACAGCTGGAG GCAAACCCCA ATTGGTGTCA 
GCTATGGTTT CAGGCACCAC TAAGACTGTT ACACTTGTAC AAAACGCTGG TGCTAACAAG 
GCAGGTGGTG TAGGGCCCGG CACTACCATC GTAAAACTTG TCTCAACCAC CCAGGCTGGA 
GGCAAGCCTA CTACCATCAT CACCCCTATC AGCCAGGCAG GGTTGAAAGG TCAGCCTACG 
ATCCTGGGTA TCAGCAGCAT CACGGCAGGT CAGCAAGGCA AAAGCATCAT TAAGACCATT 
CCAGTTGGTA AAGCAGGTGT CGCCAACACC GCAACCGGCA AGACGCCCAC CATCGTCACT 
GTAACCAACA AGATGCTGGC AGGAGCCACG GGTACTCCCA CCAAGTTTAT CACGACTGGA 
GGGGTTGGTA AGCAGCAGAA GATTGTCTTG ACAACCCAGG CAGGCGCCTC CCCTACCATC 
CTATCAGGAA CACTCAAAGG TCTAGGGGCA GGGAATTCCC CCATCACCAT CATCCGTGCT 
GTAGCGCCCC AGGGTTCGTC TCCCGGAGCC AGCGGTCAGG TCTCAGGTAA CACCATACAG 
ATCATTACCA AGGGTGGGGC TAAGAGTACC CTCAACCAGG CCAGTGCTGC TAACCTACTC 
AAATCACTCT CCACCTCTCA AGGCCTGACT ACGCCACTCA CAATCACCAC CACCGCTAAC 
TCATCGGGAG TCACAACTAA GCTGGTAACC ATTCCCATTT CAGCCCTGAC GGGGGCAGCA 
ACCACCAGCG TTTCCCCTGC CATAAACGCA ATCATGTCTA CAACAGCCCA GGTTGTCTCA 
AACACAGCTG CTGTGCAAAA GACCATCACT ACCCCTGTAG CATTGAGCAC TGTTGGTACG 
GCACAGACTA CTCTCATATC AACGGTAGGA ACAACACCTA CAGTCAGCCT TCCGAGTACA 
GATCAACAGA AGGTGGCTGA AGATGCATCA GAAACCAAAC CTACAGCTAC AGAAGAAAGC 
AAAGAAAACA AAGAAGCACA ATCAACAACA GACACAACGG CAACACCAAC TCCTACAACA 
CAAGAGGCTG CAACCCCTGC CACTACAGAG GCATCAGAGG GCGGTGTAAC GATAAAGAAG 
GAAGAGCAGA CTTCAGAACC CAAGGTAGAA TCAGCTGCTT CAGAAGCCGC CACGCCCATG 
GACACCGACC CCTCCCCTGC ACCCGGTACA GGCATACCGG CCGAGCCTAC CGATACCACC 
AAGACCAATG GCTCCACCCC TGCTGAAGAG ACGAAGAACA AGGCCTTGGA TAACGGGGCA 
GTGGGAGAAG AGCCGATGGA TACGAAAGAG AGCAACACAG ATCCAGATTC AGATCCTAAG 
TCTATTGCCA TGACAATTGC ATCACTTGCT AATTCGGCCG GTCCCCCTGT GATGTCGACC 
AGGCCTATCA CCAATGGACT GGACTCAAAA ACGGAGGATG GCAAGTCACC GATGGGGCAA 
GCCATCCAGC TAGCCAAGAA AGATGTCAAC CAGTGGTACG ACGTTGGAAT TATTAAGACA 
ACAACCACTA CAGTAGCGCA TTATCACTTG CCATCAGAGA TCATTAGCAG AAATGAAGAT 
GACATTGATG TTGTCAGTGT GCCAGATCAC AGCTTCCTCA AGAAGCAGGA GCTCCACCCG 
GGCACAGCCT ATAAGTTCAG GGTCGCTGGT ATCAATGCTA GCGGGCGGGG TCCATGGAGC 
GAGATCTCCG CATTCAAGAC ATGCTTGCCT GGATTCCCAG GGGCACCTTC AGCTATTAGA 
ATCAGCAAGA GTCCCGATGG AGCTCATCTA TCCTGGGAGC CTCCGGCAAA CACCAACGGT 
GAGATCCAGG AGTATTCTGT CTACCTTGCC GTACGTAGCA CCATGAGCGG GGCGTCGGGA 
GATGCCAAGC CCACTACCCC AGCCCAGCTA GCCTTCGTCA GGGTGTACTG CGGACCCAAC 
CCATCTTGCG TCGTGAGCTC GGCTAACCTG GCCGCAGCTC ACATCGACTG CACCAGCAAA 
CCTGCTATCA TTTTCAGAAT CGCTGCACGT AACGTCAAGG GATATGGACC AGCCACTCAG 
GTTAGATGGC TTCAAGATAA CCAGACTGCT GCTAAACCAA CAACTACTGT TGCTGCCAAG 
AGACCAGCAT CTTCTATAGA AAGTCCGATG GTGAAGAAAG TAAAAGCAGA AAACGACAGT 
GATTCCAAGA CGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011682003.1
CDS	36..3953
Translation	MDATHKLPAWYIALWVRDKMAAPILKWKRVTNTTGPSPRPRHGHRAVAIKDLMVVFGGGNEGIVDELHVYNTATNQWFVPAVRGDIPPGCAAYGFVSDGTRLFIFGGMVEYGKYSNELYELQASRWEWKRLKPKTAKNAPPPCPRLGHTFTMLGSKAYLFGGLANDSDDPKNNIPRYLNDLYSLELRSGYSNMIWDIPLTTGPAPPPRESHTVVGYAPKDGSFNRLIVYGGMSGCRLGDLWQLNMDTHTWIKPELNGPNPLPRSLHSATVIGNRMFVFGGWVPLVMDDVKVAAHEKEWKCTNTLASLNLATHTWEPLAMEVFEEAVPRARAGHCSVAIHTRLYVWSGRDGYRKAWNNQVCCKDLWFLETEKPMAPGRVQLVRASTTTLEVSWGAVATADAYLLQLQKYDMPPTSSAALSSVGGAASTKPLQSPIKAQATTTFRVVAPGSSASCPIQLIKHTPGVKIQSPTSMAGSAASSLFKLSKPLPTPGVQQQNTLSGIVALAEAAAGTQKMVSSTTAVSPTPNKPLLATVGGSNLTLVTRSMPGGVSVTLPQGMKLTTAAGGGLKMTTAGGKPQLVSAMVSGTTKTVTLVQNAGANKAGGVGPGTTIVKLVSTTQAGGKPTTIITPISQAGLKGQPTILGISSITAGQQGKSIIKTIPVGKAGVANTATGKTPTIVTVTNKMLAGATGTPTKFITTGGVGKQQKIVLTTQAGASPTILSGTLKGLGAGNSPITIIRAVAPQGSSPGASGQVSGNTIQIITKGGAKSTLNQASAANLLKSLSTSQGLTTPLTITTTANSSGVTTKLVTIPISALTGAATTSVSPAINAIMSTTAQVVSNTAAVQKTITTPVALSTVGTAQTTLISTVGTTPTVSLPSTDQQKVAEDASETKPTATEESKENKEAQSTTDTTATPTPTTQEAATPATTEASEGGVTIKKEEQTSEPKVESAASEAATPMDTDPSPAPGTGIPAEPTDTTKTNGSTPAEETKNKALDNGAVGEEPMDTKESNTDPDSDPKSIAMTIASLANSAGPPVMSTRPITNGLDSKTEDGKSPMGQAIQLAKKDVNQWYDVGIIKTTTTTVAHYHLPSEIISRNEDDIDVVSVPDHSFLKKQELHPGTAYKFRVAGINASGRGPWSEISAFKTCLPGFPGAPSAIRISKSPDGAHLSWEPPANTNGEIQEYSVYLAVRSTMSGASGDAKPTTPAQLAFVRVYCGPNPSCVVSSANLAAAHIDCTSKPAIIFRIAARNVKGYGPATQVRWLQDNQTAAKPTTTVAAKRPASSIESPMVKKVKAENDSDSKTA

Target ORF information:

RefSeq Version	XM_011683701.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011683701.1

ORF Insert Sequence:

ATGGATGCCA CGCACAAACT CCCTGCTTGG TACATTGCAT TGTGGGTAAG AGACAAAATG 
GCGGCTCCCA TACTAAAATG GAAGCGTGTG ACAAATACGA CTGGTCCAAG CCCCCGACCT 
CGCCATGGAC ATCGAGCCGT CGCAATCAAA GACCTTATGG TAGTCTTCGG CGGTGGGAAT 
GAGGGGATCG TGGATGAACT TCATGTTTAC AATACAGCTA CCAACCAGTG GTTTGTCCCA 
GCAGTGAGAG GGGATATACC CCCAGGCTGT GCAGCTTATG GCTTTGTCAG TGATGGCACC 
CGTCTCTTCA TATTTGGAGG AATGGTGGAG TATGGGAAGT ACTCCAACGA GCTATATGAA 
CTGCAAGCCA GTAGATGGGA ATGGAAACGA CTCAAGCCTA AGACTGCTAA GAATGCACCA 
CCCCCATGCC CTCGACTTGG TCACACGTTC ACCATGCTTG GGAGTAAAGC ATATCTCTTT 
GGAGGACTTG CCAATGATAG TGATGATCCA AAGAATAATA TACCAAGGTA TCTCAATGAT 
CTCTACTCAC TGGAGCTGAG ATCAGGCTAC TCGAACATGA TATGGGACAT ACCCCTAACC 
ACAGGACCTG CCCCTCCACC TAGAGAATCA CATACCGTTG TAGGTTATGC TCCCAAGGAT 
GGCTCCTTTA ATCGACTCAT TGTGTATGGT GGTATGAGCG GCTGTCGGCT CGGTGACCTT 
TGGCAACTCA ACATGGATAC ACATACATGG ATCAAACCTG AACTCAATGG ACCCAACCCA 
CTACCAAGAA GCTTGCACTC TGCCACTGTC ATCGGAAATA GAATGTTTGT GTTTGGTGGC 
TGGGTACCTC TTGTGATGGA TGATGTCAAA GTAGCAGCTC ATGAAAAGGA ATGGAAGTGT 
ACAAACACAC TGGCTTCTCT CAATCTAGCA ACCCACACAT GGGAGCCACT GGCGATGGAG 
GTGTTTGAAG AGGCTGTACC TAGAGCCAGG GCTGGGCATT GCTCTGTGGC TATCCATACC 
AGACTCTACG TATGGAGTGG AAGGGACGGA TATCGCAAGG CATGGAATAA CCAGGTGTGC 
TGCAAAGACC TTTGGTTCTT GGAGACTGAG AAGCCGATGG CCCCAGGCAG GGTCCAGCTC 
GTCCGAGCTA GCACCACCAC ACTGGAAGTA TCATGGGGTG CTGTAGCCAC AGCCGATGCC 
TATTTACTAC AGCTGCAGAA GTATGACATG CCCCCTACCT CAAGTGCAGC CTTATCCTCT 
GTGGGTGGCG CTGCTTCCAC CAAGCCACTC CAGTCACCAA TCAAAGCACA AGCAACCACC 
ACGTTCCGTG TGGTGGCTCC AGGCTCCTCA GCCTCTTGCC CTATTCAGTT GATAAAACAC 
ACTCCAGGAG TTAAGATTCA GAGTCCAACC TCCATGGCTG GCTCAGCAGC CAGTTCACTC 
TTCAAACTCA GCAAGCCCCT CCCTACTCCC GGTGTCCAGC AGCAGAACAC CCTGAGCGGT 
ATCGTAGCTC TCGCTGAGGC TGCTGCAGGC ACCCAGAAGA TGGTCAGTAG TACCACCGCT 
GTCTCACCAA CCCCCAACAA ACCGCTCCTG GCAACGGTAG GAGGGTCCAA CCTGACCCTG 
GTGACCCGGT CAATGCCAGG AGGTGTCAGC GTCACATTGC CGCAAGGGAT GAAGCTCACC 
ACGGCAGCAG GAGGTGGGCT CAAGATGACA ACAGCTGGAG GCAAACCCCA ATTGGTGTCA 
GCTATGGTTT CAGGCACCAC TAAGACTGTT ACACTTGTAC AAAACGCTGG TGCTAACAAG 
GCAGGTGGTG TAGGGCCCGG CACTACCATC GTAAAACTTG TCTCAACCAC CCAGGCTGGA 
GGCAAGCCTA CTACCATCAT CACCCCTATC AGCCAGGCAG GGTTGAAAGG TCAGCCTACG 
ATCCTGGGTA TCAGCAGCAT CACGGCAGGT CAGCAAGGCA AAAGCATCAT TAAGACCATT 
CCAGTTGGTA AAGCAGGTGT CGCCAACACC GCAACCGGCA AGACGCCCAC CATCGTCACT 
GTAACCAACA AGATGCTGGC AGGAGCCACG GGTACTCCCA CCAAGTTTAT CACGACTGGA 
GGGGTTGGTA AGCAGCAGAA GATTGTCTTG ACAACCCAGG CAGGCGCCTC CCCTACCATC 
CTATCAGGAA CACTCAAAGG TCTAGGGGCA GGGAATTCCC CCATCACCAT CATCCGTGCT 
GTAGCGCCCC AGGGTTCGTC TCCCGGAGCC AGCGGTCAGG TCTCAGGTAA CACCATACAG 
ATCATTACCA AGGGTGGGGC TAAGAGTACC CTCAACCAGG CCAGTGCTGC TAACCTACTC 
AAATCACTCT CCACCTCTCA AGGCCTGACT ACGCCACTCA CAATCACCAC CACCGCTAAC 
TCATCGGGAG TCACAACTAA GCTGGTAACC ATTCCCATTT CAGCCCTGAC GGGGGCAGCA 
ACCACCAGCG TTTCCCCTGC CATAAACGCA ATCATGTCTA CAACAGCCCA GGTTGTCTCA 
AACACAGCTG CTGTGCAAAA GACCATCACT ACCCCTGTAG CATTGAGCAC TGTTGGTACG 
GCACAGACTA CTCTCATATC AACGGTAGGA ACAACACCTA CAGTCAGCCT TCCGAGTACA 
GATCAACAGA AGGTGGCTGA AGATGCATCA GAAACCAAAC CTACAGCTAC AGAAGAAAGC 
AAAGAAAACA AAGAAGCACA ATCAACAACA GACACAACGG CAACACCAAC TCCTACAACA 
CAAGAGGCTG CAACCCCTGC CACTACAGAG GCATCAGAGG GCGGTGTAAC GATAAAGAAG 
GAAGAGCAGA CTTCAGAACC CAAGGTAGAA TCAGCTGCTT CAGAAGCCGC CACGCCCATG 
GACACCGACC CCTCCCCTGC ACCCGGTACA GGCATACCGG CCGAGCCTAC CGATACCACC 
AAGACCAATG GCTCCACCCC TGCTGAAGAG ACGAAGAACA AGGCCTTGGA TAACGGGGCA 
GTGGGAGAAG AGCCGATGGA TACGAAAGAG AGCAACACAG ATCCAGATTC AGATCCTAAG 
TCTATTGCCA TGACAATTGC ATCACTTGCT AATTCGGCCG GTCCCCCTGT GATGTCGACC 
AGGCCTATCA CCAATGGACT GGACTCAAAA ACGGAGGATG GCAAGTCACC GATGGGGCAA 
GCCATCCAGC TAGCCAAGAA AGATGTCAAC CAGTGGTACG ACGTTGGAAT TATTAAGACA 
ACAACCACTA CAGTAGCGCA TTATCACTTG CCATCAGAGA TCATTAGCAG AAATGAAGAT 
GACATTGATG TTGTCAGTGT GCCAGATCAC AGCTTCCTCA AGAAGCAGGA GCTCCACCCG 
GGCACAGCCT ATAAGTTCAG GGTCGCTGGT ATCAATGCTA GCGGGCGGGG TCCATGGAGC 
GAGATCTCCG CATTCAAGAC ATGCTTGCCT GGATTCCCAG GGGCACCTTC AGCTATTAGA 
ATCAGCAAGA GTCCCGATGG AGCTCATCTA TCCTGGGAGC CTCCGGCAAA CACCAACGGT 
GAGATCCAGG AGTATTCTGT CTACCTTGCC GTACGTAGCA CCATGAGCGG GGCGTCGGGA 
GATGCCAAGC CCACTACCCC AGCCCAGCTA GCCTTCGTCA GGGTGTACTG CGGACCCAAC 
CCATCTTGCG TCGTGAGCTC GGCTAACCTG GCCGCAGCTC ACATCGACTG CACCAGCAAA 
CCTGCTATCA TTTTCAGAAT CGCTGCACGT AACGTCAAGG GATATGGACC AGCCACTCAG 
GTTAGATGGC TTCAAGATAA CCAGACTGCT GCTAAACCAA CAACTACTGT TGCTGCCAAG 
AGACCAGCAT CTTCTATAGA AAGTCCGATG GTGAAGAAAG TAAAAGCAGA AAACGACAGT 
GATTCCAAGA CGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC586153 ( XM_785946.4 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_791039.4 Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X3, mRNA.

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CloneID	OSd25226
Clone ID Related Accession (Same CDS sequence)	XM_785946.4
Accession Version	XM_785946.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	host cell factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011994856.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 24, 2015 this sequence version replaced XM_785946.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCA CGCACAAACT CCCTGCTTGG TACATTGCAT TGTGGGTAAG AGACAAAATG 
GCGGCTCCCA TACTAAAATG GAAGCGTGTG ACAAATACGA CTGGTCCAAG CCCCCGACCT 
CGCCATGGAC ATCGAGCCGT CGCAATCAAA GACCTTATGG TAGTCTTCGG CGGTGGGAAT 
GAGGGGATCG TGGATGAACT TCATGTTTAC AATACAGCTA CCAACCAGTG GTTTGTCCCA 
GCAGTGAGAG GGGATATACC CCCAGGCTGT GCAGCTTATG GCTTTGTCAG TGATGGCACC 
CGTCTCTTCA TATTTGGAGG AATGGTGGAG TATGGGAAGT ACTCCAACGA GCTATATGAA 
CTGCAAGCCA GTAGATGGGA ATGGAAACGA CTCAAGCCTA AGACTGCTAA GAATGCACCA 
CCCCCATGCC CTCGACTTGG TCACACGTTC ACCATGCTTG GGAGTAAAGC ATATCTCTTT 
GGAGGACTTG CCAATGATAG TGATGATCCA AAGAATAATA TACCAAGGTA TCTCAATGAT 
CTCTACTCAC TGGAGCTGAG ATCAGGCTAC TCGAACATGA TATGGGACAT ACCCCTAACC 
ACAGGACCTG CCCCTCCACC TAGAGAATCA CATACCGTTG TAGGTTATGC TCCCAAGGAT 
GGCTCCTTTA ATCGACTCAT TGTGTATGGT GGTATGAGCG GCTGTCGGCT CGGTGACCTT 
TGGCAACTCA ACATGGATAC ACATACATGG ATCAAACCTG AACTCAATGG ACCCAACCCA 
CTACCAAGAA GCTTGCACTC TGCCACTGTC ATCGGAAATA GAATGTTTGT GTTTGGTGGC 
TGGGTACCTC TTGTGATGGA TGATGTCAAA GTAGCAGCTC ATGAAAAGGA ATGGAAGTGT 
ACAAACACAC TGGCTTCTCT CAATCTAGCA ACCCACACAT GGGAGCCACT GGCGATGGAG 
GTGTTTGAAG AGGCTGTACC TAGAGCCAGG GCTGGGCATT GCTCTGTGGC TATCCATACC 
AGACTCTACG TATGGAGTGG AAGGGACGGA TATCGCAAGG CATGGAATAA CCAGGTGTGC 
TGCAAAGACC TTTGGTTCTT GGAGACTGAG AAGCCGATGG CCCCAGGCAG GGTCCAGCTC 
GTCCGAGCTA GCACCACCAC ACTGGAAGTA TCATGGGGTG CTGTAGCCAC AGCCGATGCC 
TATTTACTAC AGCTGCAGAA GTATGACATG CCCCCTACCT CAAGTGCAGC CTTATCCTCT 
GTGGGTGGCG CTGCTTCCAC CAAGCCACTC CAGTCACCAA TCAAAGCACA AGCTCCAGGA 
GTTAAGATTC AGAGTCCAAC CTCCATGGCT GGCTCAGCAG CCAGTTCACT CTTCAAACTC 
AGCAAGCCCC TCCCTACTCC CGGTGTCCAG CAGCAGAACA CCCTGAGCGG TATCGTAGCT 
CTCGCTGAGG CTGCTGCAGG CACCCAGAAG ATGGTCAGTA GTACCACCGC TGTCTCACCA 
ACCCCCAACA AACCGCTCCT GGCAACGGTA GGAGGGTCCA ACCTGACCCT GGTGACCCGG 
TCAATGCCAG GAGGTGTCAG CGTCACATTG CCGCAAGGGA TGAAGCTCAC CACGGCAGCA 
GGAGGTGGGC TCAAGATGAC AACAGCTGGA GGCAAACCCC AATTGGTGTC AGCTATGGTT 
TCAGGCACCA CTAAGACTGT TACACTTGTA CAAAACGCTG GTGCTAACAA GGCAGGTGGT 
GTAGGGCCCG GCACTACCAT CGTAAAACTT GTCTCAACCA CCCAGGCTGG AGGCAAGCCT 
ACTACCATCA TCACCCCTAT CAGCCAGGCA GGGTTGAAAG GTCAGCCTAC GATCCTGGGT 
ATCAGCAGCA TCACGGCAGG TCAGCAAGGC AAAAGCATCA TTAAGACCAT TCCAGTTGGT 
AAAGCAGGTG TCGCCAACAC CGCAACCGGC AAGACGCCCA CCATCGTCAC TGTAACCAAC 
AAGATGCTGG CAGGAGCCAC GGGTACTCCC ACCAAGTTTA TCACGACTGG AGGGGTTGGT 
AAGCAGCAGA AGATTGTCTT GACAACCCAG GCAGGCGCCT CCCCTACCAT CCTATCAGGA 
ACACTCAAAG GTCTAGGGGC AGGGAATTCC CCCATCACCA TCATCCGTGC TGTAGCGCCC 
CAGGGTTCGT CTCCCGGAGC CAGCGGTCAG GTCTCAGGTA ACACCATACA GATCATTACC 
AAGGGTGGGG CTAAGAGTAC CCTCAACCAG GCCAGTGCTG CTAACCTACT CAAATCACTC 
TCCACCTCTC AAGGCCTGAC TACGCCACTC ACAATCACCA CCACCGCTAA CTCATCGGGA 
GTCACAACTA AGCTGGTAAC CATTCCCATT TCAGCCCTGA CGGGGGCAGC AACCACCAGC 
GTTTCCCCTG CCATAAACGC AATCATGTCT ACAACAGCCC AGGTTGTCTC AAACACAGCT 
GCTGTGCAAA AGACCATCAC TACCCCTGTA GCATTGAGCA CTGTTGGTAC GGCACAGACT 
ACTCTCATAT CAACGGTAGG AACAACACCT ACAGTCAGCC TTCCGAGTAC AGATCAACAG 
AAGGTGGCTG AAGATGCATC AGAAACCAAA CCTACAGCTA CAGAAGAAAG CAAAGAAAAC 
AAAGAAGCAC AATCAACAAC AGACACAACG GCAACACCAA CTCCTACAAC ACAAGAGGCT 
GCAACCCCTG CCACTACAGA GGCATCAGAG GGCGGTGTAA CGATAAAGAA GGAAGAGCAG 
ACTTCAGAAC CAAATCAACA ACCCAAGGTA GAATCAGCTG CTTCAGAAGC CGCCACGCCC 
ATGGACACCG ACCCCTCCCC TGCACCCGGT ACAGGCATAC CGGCCGAGCC TACCGATACC 
ACCAAGACCA ATGGCTCCAC CCCTGCTGAA GAGACGAAGA ACAAGGCCTT GGATAACGGG 
GCAGTGGGAG AAGAGCCGAT GGATACGAAA GAGAGCAACA CAGATCCAGA TTCAGATCCT 
AAGTCTATTG CCATGACAAT TGCATCACTT GCTAATTCGG CCGGTCCCCC TGTGATGTCG 
ACCAGGCCTA TCACCAATGG ACTGGACTCA AAAACGGAGG ATGGCAAGTC ACCGATGGGG 
CAAGCCATCC AGCTAGCCAA GAAAGATGTC AACCAGTGGT ACGACGTTGG AATTATTAAG 
ACAACAACCA CTACAGTAGC GCATTATCAC TTGCCATCAG AGATCATTAG CAGAAATGAA 
GATGACATTG ATGTTGTCAG TGTGCCAGAT CACAGCTTCC TCAAGAAGCA GGAGCTCCAC 
CCGGGCACAG CCTATAAGTT CAGGGTCGCT GGTATCAATG CTAGCGGGCG GGGTCCATGG 
AGCGAGATCT CCGCATTCAA GACATGCTTG CCTGGATTCC CAGGGGCACC TTCAGCTATT 
AGAATCAGCA AGAGTCCCGA TGGAGCTCAT CTATCCTGGG AGCCTCCGGC AAACACCAAC 
GGTGAGATCC AGGAGTATTC TGTCTACCTT GCCGTACGTA GCACCATGAG CGGGGCGTCG 
GGAGATGCCA AGCCCACTAC CCCAGCCCAG CTAGCCTTCG TCAGGGTGTA CTGCGGACCC 
AACCCATCTT GCGTCGTGAG CTCGGCTAAC CTGGCCGCAG CTCACATCGA CTGCACCAGC 
AAACCTGCTA TCATTTTCAG AATCGCTGCA CGTAACGTCA AGGGATATGG ACCAGCCACT 
CAGGTTAGAT GGCTTCAAGA TAACCAGACT GCTGCTAAAC CAACAACTAC TGTTGCTGCC 
AAGAGACCAG CATCTTCTAT AGAAAGTCCG ATGGTGAAGA AAGTAAAAGC AGAAAACGAC 
AGTGATTCCA AGACGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_791039.4
CDS	36..3896
Translation	MDATHKLPAWYIALWVRDKMAAPILKWKRVTNTTGPSPRPRHGHRAVAIKDLMVVFGGGNEGIVDELHVYNTATNQWFVPAVRGDIPPGCAAYGFVSDGTRLFIFGGMVEYGKYSNELYELQASRWEWKRLKPKTAKNAPPPCPRLGHTFTMLGSKAYLFGGLANDSDDPKNNIPRYLNDLYSLELRSGYSNMIWDIPLTTGPAPPPRESHTVVGYAPKDGSFNRLIVYGGMSGCRLGDLWQLNMDTHTWIKPELNGPNPLPRSLHSATVIGNRMFVFGGWVPLVMDDVKVAAHEKEWKCTNTLASLNLATHTWEPLAMEVFEEAVPRARAGHCSVAIHTRLYVWSGRDGYRKAWNNQVCCKDLWFLETEKPMAPGRVQLVRASTTTLEVSWGAVATADAYLLQLQKYDMPPTSSAALSSVGGAASTKPLQSPIKAQAPGVKIQSPTSMAGSAASSLFKLSKPLPTPGVQQQNTLSGIVALAEAAAGTQKMVSSTTAVSPTPNKPLLATVGGSNLTLVTRSMPGGVSVTLPQGMKLTTAAGGGLKMTTAGGKPQLVSAMVSGTTKTVTLVQNAGANKAGGVGPGTTIVKLVSTTQAGGKPTTIITPISQAGLKGQPTILGISSITAGQQGKSIIKTIPVGKAGVANTATGKTPTIVTVTNKMLAGATGTPTKFITTGGVGKQQKIVLTTQAGASPTILSGTLKGLGAGNSPITIIRAVAPQGSSPGASGQVSGNTIQIITKGGAKSTLNQASAANLLKSLSTSQGLTTPLTITTTANSSGVTTKLVTIPISALTGAATTSVSPAINAIMSTTAQVVSNTAAVQKTITTPVALSTVGTAQTTLISTVGTTPTVSLPSTDQQKVAEDASETKPTATEESKENKEAQSTTDTTATPTPTTQEAATPATTEASEGGVTIKKEEQTSEPNQQPKVESAASEAATPMDTDPSPAPGTGIPAEPTDTTKTNGSTPAEETKNKALDNGAVGEEPMDTKESNTDPDSDPKSIAMTIASLANSAGPPVMSTRPITNGLDSKTEDGKSPMGQAIQLAKKDVNQWYDVGIIKTTTTTVAHYHLPSEIISRNEDDIDVVSVPDHSFLKKQELHPGTAYKFRVAGINASGRGPWSEISAFKTCLPGFPGAPSAIRISKSPDGAHLSWEPPANTNGEIQEYSVYLAVRSTMSGASGDAKPTTPAQLAFVRVYCGPNPSCVVSSANLAAAHIDCTSKPAIIFRIAARNVKGYGPATQVRWLQDNQTAAKPTTTVAAKRPASSIESPMVKKVKAENDSDSKTA

Target ORF information:

RefSeq Version	XM_785946.4
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_785946.4

ORF Insert Sequence:

ATGGATGCCA CGCACAAACT CCCTGCTTGG TACATTGCAT TGTGGGTAAG AGACAAAATG 
GCGGCTCCCA TACTAAAATG GAAGCGTGTG ACAAATACGA CTGGTCCAAG CCCCCGACCT 
CGCCATGGAC ATCGAGCCGT CGCAATCAAA GACCTTATGG TAGTCTTCGG CGGTGGGAAT 
GAGGGGATCG TGGATGAACT TCATGTTTAC AATACAGCTA CCAACCAGTG GTTTGTCCCA 
GCAGTGAGAG GGGATATACC CCCAGGCTGT GCAGCTTATG GCTTTGTCAG TGATGGCACC 
CGTCTCTTCA TATTTGGAGG AATGGTGGAG TATGGGAAGT ACTCCAACGA GCTATATGAA 
CTGCAAGCCA GTAGATGGGA ATGGAAACGA CTCAAGCCTA AGACTGCTAA GAATGCACCA 
CCCCCATGCC CTCGACTTGG TCACACGTTC ACCATGCTTG GGAGTAAAGC ATATCTCTTT 
GGAGGACTTG CCAATGATAG TGATGATCCA AAGAATAATA TACCAAGGTA TCTCAATGAT 
CTCTACTCAC TGGAGCTGAG ATCAGGCTAC TCGAACATGA TATGGGACAT ACCCCTAACC 
ACAGGACCTG CCCCTCCACC TAGAGAATCA CATACCGTTG TAGGTTATGC TCCCAAGGAT 
GGCTCCTTTA ATCGACTCAT TGTGTATGGT GGTATGAGCG GCTGTCGGCT CGGTGACCTT 
TGGCAACTCA ACATGGATAC ACATACATGG ATCAAACCTG AACTCAATGG ACCCAACCCA 
CTACCAAGAA GCTTGCACTC TGCCACTGTC ATCGGAAATA GAATGTTTGT GTTTGGTGGC 
TGGGTACCTC TTGTGATGGA TGATGTCAAA GTAGCAGCTC ATGAAAAGGA ATGGAAGTGT 
ACAAACACAC TGGCTTCTCT CAATCTAGCA ACCCACACAT GGGAGCCACT GGCGATGGAG 
GTGTTTGAAG AGGCTGTACC TAGAGCCAGG GCTGGGCATT GCTCTGTGGC TATCCATACC 
AGACTCTACG TATGGAGTGG AAGGGACGGA TATCGCAAGG CATGGAATAA CCAGGTGTGC 
TGCAAAGACC TTTGGTTCTT GGAGACTGAG AAGCCGATGG CCCCAGGCAG GGTCCAGCTC 
GTCCGAGCTA GCACCACCAC ACTGGAAGTA TCATGGGGTG CTGTAGCCAC AGCCGATGCC 
TATTTACTAC AGCTGCAGAA GTATGACATG CCCCCTACCT CAAGTGCAGC CTTATCCTCT 
GTGGGTGGCG CTGCTTCCAC CAAGCCACTC CAGTCACCAA TCAAAGCACA AGCTCCAGGA 
GTTAAGATTC AGAGTCCAAC CTCCATGGCT GGCTCAGCAG CCAGTTCACT CTTCAAACTC 
AGCAAGCCCC TCCCTACTCC CGGTGTCCAG CAGCAGAACA CCCTGAGCGG TATCGTAGCT 
CTCGCTGAGG CTGCTGCAGG CACCCAGAAG ATGGTCAGTA GTACCACCGC TGTCTCACCA 
ACCCCCAACA AACCGCTCCT GGCAACGGTA GGAGGGTCCA ACCTGACCCT GGTGACCCGG 
TCAATGCCAG GAGGTGTCAG CGTCACATTG CCGCAAGGGA TGAAGCTCAC CACGGCAGCA 
GGAGGTGGGC TCAAGATGAC AACAGCTGGA GGCAAACCCC AATTGGTGTC AGCTATGGTT 
TCAGGCACCA CTAAGACTGT TACACTTGTA CAAAACGCTG GTGCTAACAA GGCAGGTGGT 
GTAGGGCCCG GCACTACCAT CGTAAAACTT GTCTCAACCA CCCAGGCTGG AGGCAAGCCT 
ACTACCATCA TCACCCCTAT CAGCCAGGCA GGGTTGAAAG GTCAGCCTAC GATCCTGGGT 
ATCAGCAGCA TCACGGCAGG TCAGCAAGGC AAAAGCATCA TTAAGACCAT TCCAGTTGGT 
AAAGCAGGTG TCGCCAACAC CGCAACCGGC AAGACGCCCA CCATCGTCAC TGTAACCAAC 
AAGATGCTGG CAGGAGCCAC GGGTACTCCC ACCAAGTTTA TCACGACTGG AGGGGTTGGT 
AAGCAGCAGA AGATTGTCTT GACAACCCAG GCAGGCGCCT CCCCTACCAT CCTATCAGGA 
ACACTCAAAG GTCTAGGGGC AGGGAATTCC CCCATCACCA TCATCCGTGC TGTAGCGCCC 
CAGGGTTCGT CTCCCGGAGC CAGCGGTCAG GTCTCAGGTA ACACCATACA GATCATTACC 
AAGGGTGGGG CTAAGAGTAC CCTCAACCAG GCCAGTGCTG CTAACCTACT CAAATCACTC 
TCCACCTCTC AAGGCCTGAC TACGCCACTC ACAATCACCA CCACCGCTAA CTCATCGGGA 
GTCACAACTA AGCTGGTAAC CATTCCCATT TCAGCCCTGA CGGGGGCAGC AACCACCAGC 
GTTTCCCCTG CCATAAACGC AATCATGTCT ACAACAGCCC AGGTTGTCTC AAACACAGCT 
GCTGTGCAAA AGACCATCAC TACCCCTGTA GCATTGAGCA CTGTTGGTAC GGCACAGACT 
ACTCTCATAT CAACGGTAGG AACAACACCT ACAGTCAGCC TTCCGAGTAC AGATCAACAG 
AAGGTGGCTG AAGATGCATC AGAAACCAAA CCTACAGCTA CAGAAGAAAG CAAAGAAAAC 
AAAGAAGCAC AATCAACAAC AGACACAACG GCAACACCAA CTCCTACAAC ACAAGAGGCT 
GCAACCCCTG CCACTACAGA GGCATCAGAG GGCGGTGTAA CGATAAAGAA GGAAGAGCAG 
ACTTCAGAAC CAAATCAACA ACCCAAGGTA GAATCAGCTG CTTCAGAAGC CGCCACGCCC 
ATGGACACCG ACCCCTCCCC TGCACCCGGT ACAGGCATAC CGGCCGAGCC TACCGATACC 
ACCAAGACCA ATGGCTCCAC CCCTGCTGAA GAGACGAAGA ACAAGGCCTT GGATAACGGG 
GCAGTGGGAG AAGAGCCGAT GGATACGAAA GAGAGCAACA CAGATCCAGA TTCAGATCCT 
AAGTCTATTG CCATGACAAT TGCATCACTT GCTAATTCGG CCGGTCCCCC TGTGATGTCG 
ACCAGGCCTA TCACCAATGG ACTGGACTCA AAAACGGAGG ATGGCAAGTC ACCGATGGGG 
CAAGCCATCC AGCTAGCCAA GAAAGATGTC AACCAGTGGT ACGACGTTGG AATTATTAAG 
ACAACAACCA CTACAGTAGC GCATTATCAC TTGCCATCAG AGATCATTAG CAGAAATGAA 
GATGACATTG ATGTTGTCAG TGTGCCAGAT CACAGCTTCC TCAAGAAGCA GGAGCTCCAC 
CCGGGCACAG CCTATAAGTT CAGGGTCGCT GGTATCAATG CTAGCGGGCG GGGTCCATGG 
AGCGAGATCT CCGCATTCAA GACATGCTTG CCTGGATTCC CAGGGGCACC TTCAGCTATT 
AGAATCAGCA AGAGTCCCGA TGGAGCTCAT CTATCCTGGG AGCCTCCGGC AAACACCAAC 
GGTGAGATCC AGGAGTATTC TGTCTACCTT GCCGTACGTA GCACCATGAG CGGGGCGTCG 
GGAGATGCCA AGCCCACTAC CCCAGCCCAG CTAGCCTTCG TCAGGGTGTA CTGCGGACCC 
AACCCATCTT GCGTCGTGAG CTCGGCTAAC CTGGCCGCAG CTCACATCGA CTGCACCAGC 
AAACCTGCTA TCATTTTCAG AATCGCTGCA CGTAACGTCA AGGGATATGG ACCAGCCACT 
CAGGTTAGAT GGCTTCAAGA TAACCAGACT GCTGCTAAAC CAACAACTAC TGTTGCTGCC 
AAGAGACCAG CATCTTCTAT AGAAAGTCCG ATGGTGAAGA AAGTAAAAGC AGAAAACGAC 
AGTGATTCCA AGACGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC586153 ( XM_011683702.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011682004.1 Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSd25227
Clone ID Related Accession (Same CDS sequence)	XM_011683702.1
Accession Version	XM_011683702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	host cell factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011994856.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCA CGCACAAACT CCCTGCTTGG TACATTGCAT TGTGGGTAAG AGACAAAATG 
GCGGCTCCCA TACTAAAATG GAAGCGTGTG ACAAATACGA CTGGTCCAAG CCCCCGACCT 
CGCCATGGAC ATCGAGCCGT CGCAATCAAA GACCTTATGG TAGTCTTCGG CGGTGGGAAT 
GAGGGGATCG TGGATGAACT TCATGTTTAC AATACAGCTA CCAACCAGTG GTTTGTCCCA 
GCAGTGAGAG GGGATATACC CCCAGGCTGT GCAGCTTATG GCTTTGTCAG TGATGGCACC 
CGTCTCTTCA TATTTGGAGG AATGGTGGAG TATGGGAAGT ACTCCAACGA GCTATATGAA 
CTGCAAGCCA GTAGATGGGA ATGGAAACGA CTCAAGCCTA AGACTGCTAA GAATGCACCA 
CCCCCATGCC CTCGACTTGG TCACACGTTC ACCATGCTTG GGAGTAAAGC ATATCTCTTT 
GGAGGACTTG CCAATGATAG TGATGATCCA AAGAATAATA TACCAAGGTA TCTCAATGAT 
CTCTACTCAC TGGAGCTGAG ATCAGGCTAC TCGAACATGA TATGGGACAT ACCCCTAACC 
ACAGGACCTG CCCCTCCACC TAGAGAATCA CATACCGTTG TAGGTTATGC TCCCAAGGAT 
GGCTCCTTTA ATCGACTCAT TGTGTATGGT GGTATGAGCG GCTGTCGGCT CGGTGACCTT 
TGGCAACTCA ACATGGATAC ACATACATGG ATCAAACCTG AACTCAATGG ACCCAACCCA 
CTACCAAGAA GCTTGCACTC TGCCACTGTC ATCGGAAATA GAATGTTTGT GTTTGGTGGC 
TGGGTACCTC TTGTGATGGA TGATGTCAAA GTAGCAGCTC ATGAAAAGGA ATGGAAGTGT 
ACAAACACAC TGGCTTCTCT CAATCTAGCA ACCCACACAT GGGAGCCACT GGCGATGGAG 
GTGTTTGAAG AGGCTGTACC TAGAGCCAGG GCTGGGCATT GCTCTGTGGC TATCCATACC 
AGACTCTACG TATGGAGTGG AAGGGACGGA TATCGCAAGG CATGGAATAA CCAGGTGTGC 
TGCAAAGACC TTTGGTTCTT GGAGACTGAG AAGCCGATGG CCCCAGGCAG GGTCCAGCTC 
GTCCGAGCTA GCACCACCAC ACTGGAAGTA TCATGGGGTG CTGTAGCCAC AGCCGATGCC 
TATTTACTAC AGCTGCAGAA GTATGACATG CCCCCTACCT CAAGTGCAGC CTTATCCTCT 
GTGGGTGGCG CTGCTTCCAC CAAGCCACTC CAGTCACCAA TCAAAGCACA AGCAACCACC 
ACGTTCCGTG TGGTGGCTCC AGGCTCCTCA GCCTCTTGCC CTATTCAGTT GATAAAACAC 
ACTCCAGGAG TTAAGATTCA GAGTCCAACC TCCATGGCTG GCTCAGCAGC CAGTTCACTC 
TTCAAACTCA GCAAGCCCCT CCCTACTCCC GGTGTCCAGC AGCAGAACAC CCTGAGCGGT 
ATCGTAGCTC TCGCTGAGGC TGCTGCAGGC ACCCAGAAGA TGGTCAGTAG TACCACCGCT 
GTCTCACCAA CCCCCAACAA ACCGCTCCTG GCAACGGTAG GAGGGTCCAA CCTGACCCTG 
GTGACCCGGT CAATGCCAGG AGGTGTCAGC GTCACATTGC CGCAAGGGAT GAAGCTCACC 
ACGGCAGCAG GAGGTGGGCT CAAGATGACA ACAGCTGGAG GCAAACCCCA ATTGGTGTCA 
GCTATGGTTT CAGGCACCAC TAAGACTGTT ACACTTGTAC AAAACGCTGG TGCTAACAAG 
GCAGGTGGTG TAGGGCCCGG CACTACCATC GTAAAACTTG TCTCAACCAC CCAGGCTGGA 
GGCAAGCCTA CTACCATCAT CACCCCTATC AGCCAGGCAG GGTTGAAAGG TCAGCCTACG 
ATCCTGGGTA TCAGCAGCAT CACGGCAGGT CAGCAAGGCA AAAGCATCAT TAAGACCATT 
CCAGTTGGTA AAGCAGGTGT CGCCAACACC GCAACCGGCA AGACGCCCAC CATCGTCACT 
GTAACCAACA AGATGCTGGC AGGAGCCACG GGTACTCCCA CCAAGTTTAT CACGACTGGA 
GGGGTTGGTA AGCAGCAGAA GATTGTCTTG ACAACCCAGG CAGGCGCCTC CCCTACCATC 
CTATCAGGAA CACTCAAAGG TCTAGGGGCA GGGAATTCCC CCATCACCAT CATCCGTGCT 
GTAGCGCCCC AGGGTTCGTC TCCCGGAGCC AGCGGTCAGG TCTCAGGAGT CACAACTAAG 
CTGGTAACCA TTCCCATTTC AGCCCTGACG GGGGCAGCAA CCACCAGCGT TTCCCCTGCC 
ATAAACGCAA TCATGTCTAC AACAGCCCAG GTTGTCTCAA ACACAGCTGC TGTGCAAAAG 
ACCATCACTA CCCCTGTAGC ATTGAGCACT GTTGGTACGG CACAGACTAC TCTCATATCA 
ACGGTAGGAA CAACACCTAC AGTCAGCCTT CCGAGTACAG ATCAACAGAA GGTGGCTGAA 
GATGCATCAG AAACCAAACC TACAGCTACA GAAGAAAGCA AAGAAAACAA AGAAGCACAA 
TCAACAACAG ACACAACGGC AACACCAACT CCTACAACAC AAGAGGCTGC AACCCCTGCC 
ACTACAGAGG CATCAGAGGG CGGTGTAACG ATAAAGAAGG AAGAGCAGAC TTCAGAACCA 
AATCAACAAC CCAAGGTAGA ATCAGCTGCT TCAGAAGCCG CCACGCCCAT GGACACCGAC 
CCCTCCCCTG CACCCGGTAC AGGCATACCG GCCGAGCCTA CCGATACCAC CAAGACCAAT 
GGCTCCACCC CTGCTGAAGA GACGAAGAAC AAGGCCTTGG ATAACGGGGC AGTGGGAGAA 
GAGCCGATGG ATACGAAAGA GAGCAACACA GATCCAGATT CAGATCCTAA GTCTATTGCC 
ATGACAATTG CATCACTTGC TAATTCGGCC GGTCCCCCTG TGATGTCGAC CAGGCCTATC 
ACCAATGGAC TGGACTCAAA AACGGAGGAT GGCAAGTCAC CGATGGGGCA AGCCATCCAG 
CTAGCCAAGA AAGATGTCAA CCAGTGGTAC GACGTTGGAA TTATTAAGAC AACAACCACT 
ACAGTAGCGC ATTATCACTT GCCATCAGAG ATCATTAGCA GAAATGAAGA TGACATTGAT 
GTTGTCAGTG TGCCAGATCA CAGCTTCCTC AAGAAGCAGG AGCTCCACCC GGGCACAGCC 
TATAAGTTCA GGGTCGCTGG TATCAATGCT AGCGGGCGGG GTCCATGGAG CGAGATCTCC 
GCATTCAAGA CATGCTTGCC TGGATTCCCA GGGGCACCTT CAGCTATTAG AATCAGCAAG 
AGTCCCGATG GAGCTCATCT ATCCTGGGAG CCTCCGGCAA ACACCAACGG TGAGATCCAG 
GAGTATTCTG TCTACCTTGC CGTACGTAGC ACCATGAGCG GGGCGTCGGG AGATGCCAAG 
CCCACTACCC CAGCCCAGCT AGCCTTCGTC AGGGTGTACT GCGGACCCAA CCCATCTTGC 
GTCGTGAGCT CGGCTAACCT GGCCGCAGCT CACATCGACT GCACCAGCAA ACCTGCTATC 
ATTTTCAGAA TCGCTGCACG TAACGTCAAG GGATATGGAC CAGCCACTCA GGTTAGATGG 
CTTCAAGATA ACCAGACTGC TGCTAAACCA ACAACTACTG TTGCTGCCAA GAGACCAGCA 
TCTTCTATAG AAAGTCCGAT GGTGAAGAAA GTAAAAGCAG AAAACGACAG TGATTCCAAG 
ACGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011682004.1
CDS	36..3824
Translation	MDATHKLPAWYIALWVRDKMAAPILKWKRVTNTTGPSPRPRHGHRAVAIKDLMVVFGGGNEGIVDELHVYNTATNQWFVPAVRGDIPPGCAAYGFVSDGTRLFIFGGMVEYGKYSNELYELQASRWEWKRLKPKTAKNAPPPCPRLGHTFTMLGSKAYLFGGLANDSDDPKNNIPRYLNDLYSLELRSGYSNMIWDIPLTTGPAPPPRESHTVVGYAPKDGSFNRLIVYGGMSGCRLGDLWQLNMDTHTWIKPELNGPNPLPRSLHSATVIGNRMFVFGGWVPLVMDDVKVAAHEKEWKCTNTLASLNLATHTWEPLAMEVFEEAVPRARAGHCSVAIHTRLYVWSGRDGYRKAWNNQVCCKDLWFLETEKPMAPGRVQLVRASTTTLEVSWGAVATADAYLLQLQKYDMPPTSSAALSSVGGAASTKPLQSPIKAQATTTFRVVAPGSSASCPIQLIKHTPGVKIQSPTSMAGSAASSLFKLSKPLPTPGVQQQNTLSGIVALAEAAAGTQKMVSSTTAVSPTPNKPLLATVGGSNLTLVTRSMPGGVSVTLPQGMKLTTAAGGGLKMTTAGGKPQLVSAMVSGTTKTVTLVQNAGANKAGGVGPGTTIVKLVSTTQAGGKPTTIITPISQAGLKGQPTILGISSITAGQQGKSIIKTIPVGKAGVANTATGKTPTIVTVTNKMLAGATGTPTKFITTGGVGKQQKIVLTTQAGASPTILSGTLKGLGAGNSPITIIRAVAPQGSSPGASGQVSGVTTKLVTIPISALTGAATTSVSPAINAIMSTTAQVVSNTAAVQKTITTPVALSTVGTAQTTLISTVGTTPTVSLPSTDQQKVAEDASETKPTATEESKENKEAQSTTDTTATPTPTTQEAATPATTEASEGGVTIKKEEQTSEPNQQPKVESAASEAATPMDTDPSPAPGTGIPAEPTDTTKTNGSTPAEETKNKALDNGAVGEEPMDTKESNTDPDSDPKSIAMTIASLANSAGPPVMSTRPITNGLDSKTEDGKSPMGQAIQLAKKDVNQWYDVGIIKTTTTTVAHYHLPSEIISRNEDDIDVVSVPDHSFLKKQELHPGTAYKFRVAGINASGRGPWSEISAFKTCLPGFPGAPSAIRISKSPDGAHLSWEPPANTNGEIQEYSVYLAVRSTMSGASGDAKPTTPAQLAFVRVYCGPNPSCVVSSANLAAAHIDCTSKPAIIFRIAARNVKGYGPATQVRWLQDNQTAAKPTTTVAAKRPASSIESPMVKKVKAENDSDSKTA

Target ORF information:

RefSeq Version	XM_011683702.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011683702.1

ORF Insert Sequence:

ATGGATGCCA CGCACAAACT CCCTGCTTGG TACATTGCAT TGTGGGTAAG AGACAAAATG 
GCGGCTCCCA TACTAAAATG GAAGCGTGTG ACAAATACGA CTGGTCCAAG CCCCCGACCT 
CGCCATGGAC ATCGAGCCGT CGCAATCAAA GACCTTATGG TAGTCTTCGG CGGTGGGAAT 
GAGGGGATCG TGGATGAACT TCATGTTTAC AATACAGCTA CCAACCAGTG GTTTGTCCCA 
GCAGTGAGAG GGGATATACC CCCAGGCTGT GCAGCTTATG GCTTTGTCAG TGATGGCACC 
CGTCTCTTCA TATTTGGAGG AATGGTGGAG TATGGGAAGT ACTCCAACGA GCTATATGAA 
CTGCAAGCCA GTAGATGGGA ATGGAAACGA CTCAAGCCTA AGACTGCTAA GAATGCACCA 
CCCCCATGCC CTCGACTTGG TCACACGTTC ACCATGCTTG GGAGTAAAGC ATATCTCTTT 
GGAGGACTTG CCAATGATAG TGATGATCCA AAGAATAATA TACCAAGGTA TCTCAATGAT 
CTCTACTCAC TGGAGCTGAG ATCAGGCTAC TCGAACATGA TATGGGACAT ACCCCTAACC 
ACAGGACCTG CCCCTCCACC TAGAGAATCA CATACCGTTG TAGGTTATGC TCCCAAGGAT 
GGCTCCTTTA ATCGACTCAT TGTGTATGGT GGTATGAGCG GCTGTCGGCT CGGTGACCTT 
TGGCAACTCA ACATGGATAC ACATACATGG ATCAAACCTG AACTCAATGG ACCCAACCCA 
CTACCAAGAA GCTTGCACTC TGCCACTGTC ATCGGAAATA GAATGTTTGT GTTTGGTGGC 
TGGGTACCTC TTGTGATGGA TGATGTCAAA GTAGCAGCTC ATGAAAAGGA ATGGAAGTGT 
ACAAACACAC TGGCTTCTCT CAATCTAGCA ACCCACACAT GGGAGCCACT GGCGATGGAG 
GTGTTTGAAG AGGCTGTACC TAGAGCCAGG GCTGGGCATT GCTCTGTGGC TATCCATACC 
AGACTCTACG TATGGAGTGG AAGGGACGGA TATCGCAAGG CATGGAATAA CCAGGTGTGC 
TGCAAAGACC TTTGGTTCTT GGAGACTGAG AAGCCGATGG CCCCAGGCAG GGTCCAGCTC 
GTCCGAGCTA GCACCACCAC ACTGGAAGTA TCATGGGGTG CTGTAGCCAC AGCCGATGCC 
TATTTACTAC AGCTGCAGAA GTATGACATG CCCCCTACCT CAAGTGCAGC CTTATCCTCT 
GTGGGTGGCG CTGCTTCCAC CAAGCCACTC CAGTCACCAA TCAAAGCACA AGCAACCACC 
ACGTTCCGTG TGGTGGCTCC AGGCTCCTCA GCCTCTTGCC CTATTCAGTT GATAAAACAC 
ACTCCAGGAG TTAAGATTCA GAGTCCAACC TCCATGGCTG GCTCAGCAGC CAGTTCACTC 
TTCAAACTCA GCAAGCCCCT CCCTACTCCC GGTGTCCAGC AGCAGAACAC CCTGAGCGGT 
ATCGTAGCTC TCGCTGAGGC TGCTGCAGGC ACCCAGAAGA TGGTCAGTAG TACCACCGCT 
GTCTCACCAA CCCCCAACAA ACCGCTCCTG GCAACGGTAG GAGGGTCCAA CCTGACCCTG 
GTGACCCGGT CAATGCCAGG AGGTGTCAGC GTCACATTGC CGCAAGGGAT GAAGCTCACC 
ACGGCAGCAG GAGGTGGGCT CAAGATGACA ACAGCTGGAG GCAAACCCCA ATTGGTGTCA 
GCTATGGTTT CAGGCACCAC TAAGACTGTT ACACTTGTAC AAAACGCTGG TGCTAACAAG 
GCAGGTGGTG TAGGGCCCGG CACTACCATC GTAAAACTTG TCTCAACCAC CCAGGCTGGA 
GGCAAGCCTA CTACCATCAT CACCCCTATC AGCCAGGCAG GGTTGAAAGG TCAGCCTACG 
ATCCTGGGTA TCAGCAGCAT CACGGCAGGT CAGCAAGGCA AAAGCATCAT TAAGACCATT 
CCAGTTGGTA AAGCAGGTGT CGCCAACACC GCAACCGGCA AGACGCCCAC CATCGTCACT 
GTAACCAACA AGATGCTGGC AGGAGCCACG GGTACTCCCA CCAAGTTTAT CACGACTGGA 
GGGGTTGGTA AGCAGCAGAA GATTGTCTTG ACAACCCAGG CAGGCGCCTC CCCTACCATC 
CTATCAGGAA CACTCAAAGG TCTAGGGGCA GGGAATTCCC CCATCACCAT CATCCGTGCT 
GTAGCGCCCC AGGGTTCGTC TCCCGGAGCC AGCGGTCAGG TCTCAGGAGT CACAACTAAG 
CTGGTAACCA TTCCCATTTC AGCCCTGACG GGGGCAGCAA CCACCAGCGT TTCCCCTGCC 
ATAAACGCAA TCATGTCTAC AACAGCCCAG GTTGTCTCAA ACACAGCTGC TGTGCAAAAG 
ACCATCACTA CCCCTGTAGC ATTGAGCACT GTTGGTACGG CACAGACTAC TCTCATATCA 
ACGGTAGGAA CAACACCTAC AGTCAGCCTT CCGAGTACAG ATCAACAGAA GGTGGCTGAA 
GATGCATCAG AAACCAAACC TACAGCTACA GAAGAAAGCA AAGAAAACAA AGAAGCACAA 
TCAACAACAG ACACAACGGC AACACCAACT CCTACAACAC AAGAGGCTGC AACCCCTGCC 
ACTACAGAGG CATCAGAGGG CGGTGTAACG ATAAAGAAGG AAGAGCAGAC TTCAGAACCA 
AATCAACAAC CCAAGGTAGA ATCAGCTGCT TCAGAAGCCG CCACGCCCAT GGACACCGAC 
CCCTCCCCTG CACCCGGTAC AGGCATACCG GCCGAGCCTA CCGATACCAC CAAGACCAAT 
GGCTCCACCC CTGCTGAAGA GACGAAGAAC AAGGCCTTGG ATAACGGGGC AGTGGGAGAA 
GAGCCGATGG ATACGAAAGA GAGCAACACA GATCCAGATT CAGATCCTAA GTCTATTGCC 
ATGACAATTG CATCACTTGC TAATTCGGCC GGTCCCCCTG TGATGTCGAC CAGGCCTATC 
ACCAATGGAC TGGACTCAAA AACGGAGGAT GGCAAGTCAC CGATGGGGCA AGCCATCCAG 
CTAGCCAAGA AAGATGTCAA CCAGTGGTAC GACGTTGGAA TTATTAAGAC AACAACCACT 
ACAGTAGCGC ATTATCACTT GCCATCAGAG ATCATTAGCA GAAATGAAGA TGACATTGAT 
GTTGTCAGTG TGCCAGATCA CAGCTTCCTC AAGAAGCAGG AGCTCCACCC GGGCACAGCC 
TATAAGTTCA GGGTCGCTGG TATCAATGCT AGCGGGCGGG GTCCATGGAG CGAGATCTCC 
GCATTCAAGA CATGCTTGCC TGGATTCCCA GGGGCACCTT CAGCTATTAG AATCAGCAAG 
AGTCCCGATG GAGCTCATCT ATCCTGGGAG CCTCCGGCAA ACACCAACGG TGAGATCCAG 
GAGTATTCTG TCTACCTTGC CGTACGTAGC ACCATGAGCG GGGCGTCGGG AGATGCCAAG 
CCCACTACCC CAGCCCAGCT AGCCTTCGTC AGGGTGTACT GCGGACCCAA CCCATCTTGC 
GTCGTGAGCT CGGCTAACCT GGCCGCAGCT CACATCGACT GCACCAGCAA ACCTGCTATC 
ATTTTCAGAA TCGCTGCACG TAACGTCAAG GGATATGGAC CAGCCACTCA GGTTAGATGG 
CTTCAAGATA ACCAGACTGC TGCTAAACCA ACAACTACTG TTGCTGCCAA GAGACCAGCA 
TCTTCTATAG AAAGTCCGAT GGTGAAGAAA GTAAAAGCAG AAAACGACAG TGATTCCAAG 
ACGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC586153 ( XM_031000419.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030856279.1 Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd66402
Clone ID Related Accession (Same CDS sequence)	XM_031000419.1
Accession Version	XM_031000419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	host cell factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC CCATACTAAA ATGGAAGCGT GTGACAAATA CGACTGGTCC AAGCCCCCGA 
CCTCGCCATG GACATCGAGC CGTCGCAATC AAAGACCTTA TGGTAGTCTT CGGCGGTGGG 
AATGAGGGGA TCGTGGATGA ACTTCATGTT TACAATACAG CTACCAACCA GTGGTTTGTC 
CCAGCAGTGA GAGGGGATAT ACCCCCAGGC TGTGCAGCTT ATGGCTTTGT CAGTGATGGC 
ACCCGTCTCT TCATATTTGG AGGAATGGTG GAGTATGGGA AGTACTCCAA CGAGCTATAT 
GAACTGCAAG CCAGTAGATG GGAATGGAAA CGACTCAAGC CTAAGACTGC TAAGAATGCA 
CCACCCCCAT GCCCTCGACT TGGTCACACG TTCACCATGC TTGGGAGTAA AGCATATCTC 
TTTGGAGGAC TTGCCAATGA TAGTGATGAT CCAAAGAATA ATATACCAAG GTATCTCAAT 
GATCTCTACT CACTGGAGCT GAGATCAGGC TACTCGAACA TGATATGGGA CATACCCCTA 
ACCACAGGAC CTGCCCCTCC ACCTAGAGAA TCACATACCG TCGTAGGTTA TGCTCCAAAG 
GATGGCTCCT TTAATCGACT CATTGTATAT GGTGGTATGA GCGGTTGTCG GCTCGGTGAC 
CTTTGGCAAC TCAACATGGA TACACATACA TGGATCAAAC CTGAACTCAA TGGACCCAAC 
CCACTACCAA GAAGCTTGCA CTCTGCCACT GTCATCGGAA ATAGAATGTT TGTGTTTGGT 
GGCTGGGTAC CTCTTGTGAT GGATGATGTC AAAGTAGCAG CTCATGAAAA GGAATGGAAG 
TGTACAAACA CACTGGCTTC TCTCAATCTA GCAACCCACA CATGGGAGCC ACTGGCGATG 
GAGGTGTTTG AAGAGGCTGT ACCTAGAGCC AGAGCTGGGC ATTGCTCTGT TGCTATCCAT 
ACCAGACTCT ACGTATGGAG TGGAAGGGAC GGATATCGCA AGGCTTGGAA TAACCAGGTG 
TGCTGCAAAG ACCTTTGGTT CTTGGAGACT GAGAAGCCCA TGGCCCCAGG CAGGGTCCAG 
CTAGTCCGAG CCAGCACCAC CACGCTGGAA GTATCATGGG GTGCTGTAGC CACAGCCGAT 
GCCTATTTAC TACAGCTGCA GAAGTATGAC ATGCCCCCTA CCTCAAGTGC AGCCTTATCC 
TCTGTGGGTG GCGCTGCTTC CACCAAGCCA CTCCAGTCAC CAATCAAAGC ACAAGCAACC 
ACCACGTTCC GTGTGGTGGC TCCAGGCTCC TCAGCCTCTT GCCCTATTCA GTTGATAAAA 
CACACTCCAG GAGTTAAGAT TCAGAGTCCA ACCTCGATGG CTGGCTCAGC AGCCAGTTCA 
CTCTTTAAAC TCAGCAAGCC CCTCCCTACT CCCGGCGTCC AGCAGCAGAA CACCCTGAGC 
GGTATCGTAG CTCTCGCTGA GGCTGCTGCA GGCACCCAGA AGATGGTCAG TAGTACCACC 
GCTGTCTCAC CAACCCCCAA CAAACCGCTC CTGGCAACGG TAGGAGGGTC CAACCTGACC 
CTGGTGACCC GGTCCATGCC AGGAGGTGTC AGCGTCACAT TGCCGCAAGG GATGAAGCTC 
ACCACGGCAG CAGGAGGCGG GCTCAAGATG ACGACGGCTG GAGGCAAACC CCAATTGGTG 
TCAGCTATGG TTTCAGGCAC CACTAAAACT GTTACACTTG TGCAAAACGC TGGTGCTAAC 
AAGGCAGGTG GTGTAGGGCC TGGCACTACC ATCGTAAAAC TTGTCTCAAC CACCCAGGCT 
GGAGGCAAGC CTACTACCAT CATCACCCCT ATCAGCCAGG CGGGGTTGAA AGGTCAACCT 
ACGATCCTGG GTATCAGCAG CATCACGGCA GGTCAGCAAG GCAAAAGCAT CATCAAGACC 
ATTCCAGTTG GTAAAGCAGG TGTCGCCAAC ACCGCAACCG GCAAGACGCC CACCATCGTC 
ACTGTAACCA ACAAGATGCT GGCAGGAGCC ACGGGTACTC CCACCAAGTT TATCACGACT 
GGAGGGGTTG GTAAGCAGCA GAAGATTGTC TTGACAACCC AAGCAGGCGC CTCCCCTACC 
ATCCTATCAG GAACACTCAA AGGTCTAGGG GCAGGGAATT CCCCCATCAC CATCATCCGT 
GCTGTAGCGC CCCAGGGTTC GTCTCCCGGA GCCAGCGGTC AGGTCTCAGG TAACACCATA 
CAGATCATTA CCAAGGGTGG GGCTAAGAGT ACCCTCAACC AGGCCAGTGC TGCTAACCTA 
CTCAAATCAC TCTCCACCTC TCAAGGCCTG ACTACGCCAC TCACAATCAC CACCACCGCT 
AACTCATCGG GAGTCACAAC TAAGCTGGTA ACCATTCCCA TTTCAGCCCT GACGGGGGCA 
GCAACCACCA GCGTTTCCCC TGCCATAAAC GCAATCATGT CTACAACAGC CCAGGTTGTC 
TCAAACACAG CAGCTGTGCA AAAGACCATC ACTACCCCTG TAGCATTGAG CACTGTTGGT 
ACAGCACAGA CTACTCTCAT ATCAACGGTA GGAACAACAC CTACAGTCAG CCTTCCGAGT 
ACAGATCAAC AGAAGGTGGC TGAAGATGCA TCAGAAACCA AACCTACAGC TGCAGAAGAA 
AGCAAAGAAA ACAAAGAAGC ACAATCAACA ACAGACACAA CGGCAACACC AACTCCTACA 
ACACAAGAGG CTGCAACCCC TGCCACTACA GAGGCATCAG AGGGCGGTGT AACGATAAAG 
AAGGAAGAGC AGACTTCAGA ACCCAATCAA CAACCCAAGG TAGAATCGGC TGCTTCAGAA 
GCCACGCCCA TGGAAACCGA CCCCTCCCCT GCACCCGGAA CAGGCATACC AGCCGAGCCT 
ACCGATACCA CCAAGACCAA TGGCTCCGCC CCTGCTGAAG AGACAAAGAA CAAGGCCTTG 
GATAACGGGG CTGTGGGAGA AGAGCCGATG GATACGAAGG AGAGCAACCC AGATCCAGAT 
TCAGATCCTA AGTCTATTGC CATGACGATT GCATCACTTG CTAACTCGGC CGGTCCTCCT 
GTGATGTCGA CCAGGCCTAT CACCAATGGA CTGGACTCTA AAACGGAGGA TGGCAAGTCA 
TCGATAGGAC AAGCCATCCA GCTAGCCAAG AAAGATGTCA ACCAGTGGTA CGACGTTGGA 
ATCATTAAGA CGACGACCAC TACAGTAGCG CATTATCACT TGCCATCGGA GATCATTAGC 
AGAAATGAAG ATGACATTGA TGTTGTCAGT GTGCCGGATC ACAGCTTCCT CAAGAAGCAG 
GAGCTTCACC CGGGCACAGC CTACAAGTTC AGGGTCGCTG GTATCAATGC TAGCGGGCGG 
GGCCCATGGA GCGAGATCTC CGCGTTCAAG ACATGCTTGC CTGGATTCCC TGGGGCACCT 
TCAGCTATTA GAATCAGCAA GAGTCCCGAT GGAGCTCATC TATCCTGGGA GCCTCCGGCA 
AACACCAACG GTGAGATCCA GGAGTATTCC GTCTACCTTG CCGTACGTAG CACCATGAGC 
GGGGCGTCGG GAGATGCCAA GCCCACTACC CCAGCCCAGC TAGCCTTCGT CAGGGTATAC 
TGCGGACCCA ACCCATCTTG CGTCGTGAGC TCGGCTAACC TGGCCGCAGC TCACATCGAC 
TGCACCAGCA AACCTGCTAT CATTTTCAGA ATCGCTGCAC GTAACGTCAA GGGATATGGA 
CCAGCCACTC AGGTTAGATG GCTTCAAGAT AACCAGACTG CTGCTAAACC AACAACTACT 
GTCGCTGCCA AGAGACCAGC ATCTTCTATA GAAAGTCCGA TGGTGAAGAA AGTAAAAGCA 
GAAAACGACA GTGATTCCAA GACGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030856279.1
CDS	29..3898
Translation	MAAPILKWKRVTNTTGPSPRPRHGHRAVAIKDLMVVFGGGNEGIVDELHVYNTATNQWFVPAVRGDIPPGCAAYGFVSDGTRLFIFGGMVEYGKYSNELYELQASRWEWKRLKPKTAKNAPPPCPRLGHTFTMLGSKAYLFGGLANDSDDPKNNIPRYLNDLYSLELRSGYSNMIWDIPLTTGPAPPPRESHTVVGYAPKDGSFNRLIVYGGMSGCRLGDLWQLNMDTHTWIKPELNGPNPLPRSLHSATVIGNRMFVFGGWVPLVMDDVKVAAHEKEWKCTNTLASLNLATHTWEPLAMEVFEEAVPRARAGHCSVAIHTRLYVWSGRDGYRKAWNNQVCCKDLWFLETEKPMAPGRVQLVRASTTTLEVSWGAVATADAYLLQLQKYDMPPTSSAALSSVGGAASTKPLQSPIKAQATTTFRVVAPGSSASCPIQLIKHTPGVKIQSPTSMAGSAASSLFKLSKPLPTPGVQQQNTLSGIVALAEAAAGTQKMVSSTTAVSPTPNKPLLATVGGSNLTLVTRSMPGGVSVTLPQGMKLTTAAGGGLKMTTAGGKPQLVSAMVSGTTKTVTLVQNAGANKAGGVGPGTTIVKLVSTTQAGGKPTTIITPISQAGLKGQPTILGISSITAGQQGKSIIKTIPVGKAGVANTATGKTPTIVTVTNKMLAGATGTPTKFITTGGVGKQQKIVLTTQAGASPTILSGTLKGLGAGNSPITIIRAVAPQGSSPGASGQVSGNTIQIITKGGAKSTLNQASAANLLKSLSTSQGLTTPLTITTTANSSGVTTKLVTIPISALTGAATTSVSPAINAIMSTTAQVVSNTAAVQKTITTPVALSTVGTAQTTLISTVGTTPTVSLPSTDQQKVAEDASETKPTAAEESKENKEAQSTTDTTATPTPTTQEAATPATTEASEGGVTIKKEEQTSEPNQQPKVESAASEATPMETDPSPAPGTGIPAEPTDTTKTNGSAPAEETKNKALDNGAVGEEPMDTKESNPDPDSDPKSIAMTIASLANSAGPPVMSTRPITNGLDSKTEDGKSSIGQAIQLAKKDVNQWYDVGIIKTTTTTVAHYHLPSEIISRNEDDIDVVSVPDHSFLKKQELHPGTAYKFRVAGINASGRGPWSEISAFKTCLPGFPGAPSAIRISKSPDGAHLSWEPPANTNGEIQEYSVYLAVRSTMSGASGDAKPTTPAQLAFVRVYCGPNPSCVVSSANLAAAHIDCTSKPAIIFRIAARNVKGYGPATQVRWLQDNQTAAKPTTTVAAKRPASSIESPMVKKVKAENDSDSKTA

Target ORF information:

RefSeq Version	XM_031000419.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031000419.1

ORF Insert Sequence:

ATGGCGGCTC CCATACTAAA ATGGAAGCGT GTGACAAATA CGACTGGTCC AAGCCCCCGA 
CCTCGCCATG GACATCGAGC CGTCGCAATC AAAGACCTTA TGGTAGTCTT CGGCGGTGGG 
AATGAGGGGA TCGTGGATGA ACTTCATGTT TACAATACAG CTACCAACCA GTGGTTTGTC 
CCAGCAGTGA GAGGGGATAT ACCCCCAGGC TGTGCAGCTT ATGGCTTTGT CAGTGATGGC 
ACCCGTCTCT TCATATTTGG AGGAATGGTG GAGTATGGGA AGTACTCCAA CGAGCTATAT 
GAACTGCAAG CCAGTAGATG GGAATGGAAA CGACTCAAGC CTAAGACTGC TAAGAATGCA 
CCACCCCCAT GCCCTCGACT TGGTCACACG TTCACCATGC TTGGGAGTAA AGCATATCTC 
TTTGGAGGAC TTGCCAATGA TAGTGATGAT CCAAAGAATA ATATACCAAG GTATCTCAAT 
GATCTCTACT CACTGGAGCT GAGATCAGGC TACTCGAACA TGATATGGGA CATACCCCTA 
ACCACAGGAC CTGCCCCTCC ACCTAGAGAA TCACATACCG TCGTAGGTTA TGCTCCAAAG 
GATGGCTCCT TTAATCGACT CATTGTATAT GGTGGTATGA GCGGTTGTCG GCTCGGTGAC 
CTTTGGCAAC TCAACATGGA TACACATACA TGGATCAAAC CTGAACTCAA TGGACCCAAC 
CCACTACCAA GAAGCTTGCA CTCTGCCACT GTCATCGGAA ATAGAATGTT TGTGTTTGGT 
GGCTGGGTAC CTCTTGTGAT GGATGATGTC AAAGTAGCAG CTCATGAAAA GGAATGGAAG 
TGTACAAACA CACTGGCTTC TCTCAATCTA GCAACCCACA CATGGGAGCC ACTGGCGATG 
GAGGTGTTTG AAGAGGCTGT ACCTAGAGCC AGAGCTGGGC ATTGCTCTGT TGCTATCCAT 
ACCAGACTCT ACGTATGGAG TGGAAGGGAC GGATATCGCA AGGCTTGGAA TAACCAGGTG 
TGCTGCAAAG ACCTTTGGTT CTTGGAGACT GAGAAGCCCA TGGCCCCAGG CAGGGTCCAG 
CTAGTCCGAG CCAGCACCAC CACGCTGGAA GTATCATGGG GTGCTGTAGC CACAGCCGAT 
GCCTATTTAC TACAGCTGCA GAAGTATGAC ATGCCCCCTA CCTCAAGTGC AGCCTTATCC 
TCTGTGGGTG GCGCTGCTTC CACCAAGCCA CTCCAGTCAC CAATCAAAGC ACAAGCAACC 
ACCACGTTCC GTGTGGTGGC TCCAGGCTCC TCAGCCTCTT GCCCTATTCA GTTGATAAAA 
CACACTCCAG GAGTTAAGAT TCAGAGTCCA ACCTCGATGG CTGGCTCAGC AGCCAGTTCA 
CTCTTTAAAC TCAGCAAGCC CCTCCCTACT CCCGGCGTCC AGCAGCAGAA CACCCTGAGC 
GGTATCGTAG CTCTCGCTGA GGCTGCTGCA GGCACCCAGA AGATGGTCAG TAGTACCACC 
GCTGTCTCAC CAACCCCCAA CAAACCGCTC CTGGCAACGG TAGGAGGGTC CAACCTGACC 
CTGGTGACCC GGTCCATGCC AGGAGGTGTC AGCGTCACAT TGCCGCAAGG GATGAAGCTC 
ACCACGGCAG CAGGAGGCGG GCTCAAGATG ACGACGGCTG GAGGCAAACC CCAATTGGTG 
TCAGCTATGG TTTCAGGCAC CACTAAAACT GTTACACTTG TGCAAAACGC TGGTGCTAAC 
AAGGCAGGTG GTGTAGGGCC TGGCACTACC ATCGTAAAAC TTGTCTCAAC CACCCAGGCT 
GGAGGCAAGC CTACTACCAT CATCACCCCT ATCAGCCAGG CGGGGTTGAA AGGTCAACCT 
ACGATCCTGG GTATCAGCAG CATCACGGCA GGTCAGCAAG GCAAAAGCAT CATCAAGACC 
ATTCCAGTTG GTAAAGCAGG TGTCGCCAAC ACCGCAACCG GCAAGACGCC CACCATCGTC 
ACTGTAACCA ACAAGATGCT GGCAGGAGCC ACGGGTACTC CCACCAAGTT TATCACGACT 
GGAGGGGTTG GTAAGCAGCA GAAGATTGTC TTGACAACCC AAGCAGGCGC CTCCCCTACC 
ATCCTATCAG GAACACTCAA AGGTCTAGGG GCAGGGAATT CCCCCATCAC CATCATCCGT 
GCTGTAGCGC CCCAGGGTTC GTCTCCCGGA GCCAGCGGTC AGGTCTCAGG TAACACCATA 
CAGATCATTA CCAAGGGTGG GGCTAAGAGT ACCCTCAACC AGGCCAGTGC TGCTAACCTA 
CTCAAATCAC TCTCCACCTC TCAAGGCCTG ACTACGCCAC TCACAATCAC CACCACCGCT 
AACTCATCGG GAGTCACAAC TAAGCTGGTA ACCATTCCCA TTTCAGCCCT GACGGGGGCA 
GCAACCACCA GCGTTTCCCC TGCCATAAAC GCAATCATGT CTACAACAGC CCAGGTTGTC 
TCAAACACAG CAGCTGTGCA AAAGACCATC ACTACCCCTG TAGCATTGAG CACTGTTGGT 
ACAGCACAGA CTACTCTCAT ATCAACGGTA GGAACAACAC CTACAGTCAG CCTTCCGAGT 
ACAGATCAAC AGAAGGTGGC TGAAGATGCA TCAGAAACCA AACCTACAGC TGCAGAAGAA 
AGCAAAGAAA ACAAAGAAGC ACAATCAACA ACAGACACAA CGGCAACACC AACTCCTACA 
ACACAAGAGG CTGCAACCCC TGCCACTACA GAGGCATCAG AGGGCGGTGT AACGATAAAG 
AAGGAAGAGC AGACTTCAGA ACCCAATCAA CAACCCAAGG TAGAATCGGC TGCTTCAGAA 
GCCACGCCCA TGGAAACCGA CCCCTCCCCT GCACCCGGAA CAGGCATACC AGCCGAGCCT 
ACCGATACCA CCAAGACCAA TGGCTCCGCC CCTGCTGAAG AGACAAAGAA CAAGGCCTTG 
GATAACGGGG CTGTGGGAGA AGAGCCGATG GATACGAAGG AGAGCAACCC AGATCCAGAT 
TCAGATCCTA AGTCTATTGC CATGACGATT GCATCACTTG CTAACTCGGC CGGTCCTCCT 
GTGATGTCGA CCAGGCCTAT CACCAATGGA CTGGACTCTA AAACGGAGGA TGGCAAGTCA 
TCGATAGGAC AAGCCATCCA GCTAGCCAAG AAAGATGTCA ACCAGTGGTA CGACGTTGGA 
ATCATTAAGA CGACGACCAC TACAGTAGCG CATTATCACT TGCCATCGGA GATCATTAGC 
AGAAATGAAG ATGACATTGA TGTTGTCAGT GTGCCGGATC ACAGCTTCCT CAAGAAGCAG 
GAGCTTCACC CGGGCACAGC CTACAAGTTC AGGGTCGCTG GTATCAATGC TAGCGGGCGG 
GGCCCATGGA GCGAGATCTC CGCGTTCAAG ACATGCTTGC CTGGATTCCC TGGGGCACCT 
TCAGCTATTA GAATCAGCAA GAGTCCCGAT GGAGCTCATC TATCCTGGGA GCCTCCGGCA 
AACACCAACG GTGAGATCCA GGAGTATTCC GTCTACCTTG CCGTACGTAG CACCATGAGC 
GGGGCGTCGG GAGATGCCAA GCCCACTACC CCAGCCCAGC TAGCCTTCGT CAGGGTATAC 
TGCGGACCCA ACCCATCTTG CGTCGTGAGC TCGGCTAACC TGGCCGCAGC TCACATCGAC 
TGCACCAGCA AACCTGCTAT CATTTTCAGA ATCGCTGCAC GTAACGTCAA GGGATATGGA 
CCAGCCACTC AGGTTAGATG GCTTCAAGAT AACCAGACTG CTGCTAAACC AACAACTACT 
GTCGCTGCCA AGAGACCAGC ATCTTCTATA GAAAGTCCGA TGGTGAAGAA AGTAAAAGCA 
GAAAACGACA GTGATTCCAA GACGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC586153 ( XM_031000420.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030856280.1 Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X2, mRNA.

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CloneID	OSd66403
Clone ID Related Accession (Same CDS sequence)	XM_031000420.1
Accession Version	XM_031000420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	host cell factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC CCATACTAAA ATGGAAGCGT GTGACAAATA CGACTGGTCC AAGCCCCCGA 
CCTCGCCATG GACATCGAGC CGTCGCAATC AAAGACCTTA TGGTAGTCTT CGGCGGTGGG 
AATGAGGGGA TCGTGGATGA ACTTCATGTT TACAATACAG CTACCAACCA GTGGTTTGTC 
CCAGCAGTGA GAGGGGATAT ACCCCCAGGC TGTGCAGCTT ATGGCTTTGT CAGTGATGGC 
ACCCGTCTCT TCATATTTGG AGGAATGGTG GAGTATGGGA AGTACTCCAA CGAGCTATAT 
GAACTGCAAG CCAGTAGATG GGAATGGAAA CGACTCAAGC CTAAGACTGC TAAGAATGCA 
CCACCCCCAT GCCCTCGACT TGGTCACACG TTCACCATGC TTGGGAGTAA AGCATATCTC 
TTTGGAGGAC TTGCCAATGA TAGTGATGAT CCAAAGAATA ATATACCAAG GTATCTCAAT 
GATCTCTACT CACTGGAGCT GAGATCAGGC TACTCGAACA TGATATGGGA CATACCCCTA 
ACCACAGGAC CTGCCCCTCC ACCTAGAGAA TCACATACCG TCGTAGGTTA TGCTCCAAAG 
GATGGCTCCT TTAATCGACT CATTGTATAT GGTGGTATGA GCGGTTGTCG GCTCGGTGAC 
CTTTGGCAAC TCAACATGGA TACACATACA TGGATCAAAC CTGAACTCAA TGGACCCAAC 
CCACTACCAA GAAGCTTGCA CTCTGCCACT GTCATCGGAA ATAGAATGTT TGTGTTTGGT 
GGCTGGGTAC CTCTTGTGAT GGATGATGTC AAAGTAGCAG CTCATGAAAA GGAATGGAAG 
TGTACAAACA CACTGGCTTC TCTCAATCTA GCAACCCACA CATGGGAGCC ACTGGCGATG 
GAGGTGTTTG AAGAGGCTGT ACCTAGAGCC AGAGCTGGGC ATTGCTCTGT TGCTATCCAT 
ACCAGACTCT ACGTATGGAG TGGAAGGGAC GGATATCGCA AGGCTTGGAA TAACCAGGTG 
TGCTGCAAAG ACCTTTGGTT CTTGGAGACT GAGAAGCCCA TGGCCCCAGG CAGGGTCCAG 
CTAGTCCGAG CCAGCACCAC CACGCTGGAA GTATCATGGG GTGCTGTAGC CACAGCCGAT 
GCCTATTTAC TACAGCTGCA GAAGTATGAC ATGCCCCCTA CCTCAAGTGC AGCCTTATCC 
TCTGTGGGTG GCGCTGCTTC CACCAAGCCA CTCCAGTCAC CAATCAAAGC ACAAGCAACC 
ACCACGTTCC GTGTGGTGGC TCCAGGCTCC TCAGCCTCTT GCCCTATTCA GTTGATAAAA 
CACACTCCAG GAGTTAAGAT TCAGAGTCCA ACCTCGATGG CTGGCTCAGC AGCCAGTTCA 
CTCTTTAAAC TCAGCAAGCC CCTCCCTACT CCCGGCGTCC AGCAGCAGAA CACCCTGAGC 
GGTATCGTAG CTCTCGCTGA GGCTGCTGCA GGCACCCAGA AGATGGTCAG TAGTACCACC 
GCTGTCTCAC CAACCCCCAA CAAACCGCTC CTGGCAACGG TAGGAGGGTC CAACCTGACC 
CTGGTGACCC GGTCCATGCC AGGAGGTGTC AGCGTCACAT TGCCGCAAGG GATGAAGCTC 
ACCACGGCAG CAGGAGGCGG GCTCAAGATG ACGACGGCTG GAGGCAAACC CCAATTGGTG 
TCAGCTATGG TTTCAGGCAC CACTAAAACT GTTACACTTG TGCAAAACGC TGGTGCTAAC 
AAGGCAGGTG GTGTAGGGCC TGGCACTACC ATCGTAAAAC TTGTCTCAAC CACCCAGGCT 
GGAGGCAAGC CTACTACCAT CATCACCCCT ATCAGCCAGG CGGGGTTGAA AGGTCAACCT 
ACGATCCTGG GTATCAGCAG CATCACGGCA GGTCAGCAAG GCAAAAGCAT CATCAAGACC 
ATTCCAGTTG GTAAAGGTGT CGCCAACACC GCAACCGGCA AGACGCCCAC CATCGTCACT 
GTAACCAACA AGATGCTGGC AGGAGCCACG GGTACTCCCA CCAAGTTTAT CACGACTGGA 
GGGGTTGGTA AGCAGCAGAA GATTGTCTTG ACAACCCAAG CAGGCGCCTC CCCTACCATC 
CTATCAGGAA CACTCAAAGG TCTAGGGGCA GGGAATTCCC CCATCACCAT CATCCGTGCT 
GTAGCGCCCC AGGGTTCGTC TCCCGGAGCC AGCGGTCAGG TCTCAGGTAA CACCATACAG 
ATCATTACCA AGGGTGGGGC TAAGAGTACC CTCAACCAGG CCAGTGCTGC TAACCTACTC 
AAATCACTCT CCACCTCTCA AGGCCTGACT ACGCCACTCA CAATCACCAC CACCGCTAAC 
TCATCGGGAG TCACAACTAA GCTGGTAACC ATTCCCATTT CAGCCCTGAC GGGGGCAGCA 
ACCACCAGCG TTTCCCCTGC CATAAACGCA ATCATGTCTA CAACAGCCCA GGTTGTCTCA 
AACACAGCAG CTGTGCAAAA GACCATCACT ACCCCTGTAG CATTGAGCAC TGTTGGTACA 
GCACAGACTA CTCTCATATC AACGGTAGGA ACAACACCTA CAGTCAGCCT TCCGAGTACA 
GATCAACAGA AGGTGGCTGA AGATGCATCA GAAACCAAAC CTACAGCTGC AGAAGAAAGC 
AAAGAAAACA AAGAAGCACA ATCAACAACA GACACAACGG CAACACCAAC TCCTACAACA 
CAAGAGGCTG CAACCCCTGC CACTACAGAG GCATCAGAGG GCGGTGTAAC GATAAAGAAG 
GAAGAGCAGA CTTCAGAACC CAATCAACAA CCCAAGGTAG AATCGGCTGC TTCAGAAGCC 
ACGCCCATGG AAACCGACCC CTCCCCTGCA CCCGGAACAG GCATACCAGC CGAGCCTACC 
GATACCACCA AGACCAATGG CTCCGCCCCT GCTGAAGAGA CAAAGAACAA GGCCTTGGAT 
AACGGGGCTG TGGGAGAAGA GCCGATGGAT ACGAAGGAGA GCAACCCAGA TCCAGATTCA 
GATCCTAAGT CTATTGCCAT GACGATTGCA TCACTTGCTA ACTCGGCCGG TCCTCCTGTG 
ATGTCGACCA GGCCTATCAC CAATGGACTG GACTCTAAAA CGGAGGATGG CAAGTCATCG 
ATAGGACAAG CCATCCAGCT AGCCAAGAAA GATGTCAACC AGTGGTACGA CGTTGGAATC 
ATTAAGACGA CGACCACTAC AGTAGCGCAT TATCACTTGC CATCGGAGAT CATTAGCAGA 
AATGAAGATG ACATTGATGT TGTCAGTGTG CCGGATCACA GCTTCCTCAA GAAGCAGGAG 
CTTCACCCGG GCACAGCCTA CAAGTTCAGG GTCGCTGGTA TCAATGCTAG CGGGCGGGGC 
CCATGGAGCG AGATCTCCGC GTTCAAGACA TGCTTGCCTG GATTCCCTGG GGCACCTTCA 
GCTATTAGAA TCAGCAAGAG TCCCGATGGA GCTCATCTAT CCTGGGAGCC TCCGGCAAAC 
ACCAACGGTG AGATCCAGGA GTATTCCGTC TACCTTGCCG TACGTAGCAC CATGAGCGGG 
GCGTCGGGAG ATGCCAAGCC CACTACCCCA GCCCAGCTAG CCTTCGTCAG GGTATACTGC 
GGACCCAACC CATCTTGCGT CGTGAGCTCG GCTAACCTGG CCGCAGCTCA CATCGACTGC 
ACCAGCAAAC CTGCTATCAT TTTCAGAATC GCTGCACGTA ACGTCAAGGG ATATGGACCA 
GCCACTCAGG TTAGATGGCT TCAAGATAAC CAGACTGCTG CTAAACCAAC AACTACTGTC 
GCTGCCAAGA GACCAGCATC TTCTATAGAA AGTCCGATGG TGAAGAAAGT AAAAGCAGAA 
AACGACAGTG ATTCCAAGAC GGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030856280.1
CDS	29..3895
Translation	MAAPILKWKRVTNTTGPSPRPRHGHRAVAIKDLMVVFGGGNEGIVDELHVYNTATNQWFVPAVRGDIPPGCAAYGFVSDGTRLFIFGGMVEYGKYSNELYELQASRWEWKRLKPKTAKNAPPPCPRLGHTFTMLGSKAYLFGGLANDSDDPKNNIPRYLNDLYSLELRSGYSNMIWDIPLTTGPAPPPRESHTVVGYAPKDGSFNRLIVYGGMSGCRLGDLWQLNMDTHTWIKPELNGPNPLPRSLHSATVIGNRMFVFGGWVPLVMDDVKVAAHEKEWKCTNTLASLNLATHTWEPLAMEVFEEAVPRARAGHCSVAIHTRLYVWSGRDGYRKAWNNQVCCKDLWFLETEKPMAPGRVQLVRASTTTLEVSWGAVATADAYLLQLQKYDMPPTSSAALSSVGGAASTKPLQSPIKAQATTTFRVVAPGSSASCPIQLIKHTPGVKIQSPTSMAGSAASSLFKLSKPLPTPGVQQQNTLSGIVALAEAAAGTQKMVSSTTAVSPTPNKPLLATVGGSNLTLVTRSMPGGVSVTLPQGMKLTTAAGGGLKMTTAGGKPQLVSAMVSGTTKTVTLVQNAGANKAGGVGPGTTIVKLVSTTQAGGKPTTIITPISQAGLKGQPTILGISSITAGQQGKSIIKTIPVGKGVANTATGKTPTIVTVTNKMLAGATGTPTKFITTGGVGKQQKIVLTTQAGASPTILSGTLKGLGAGNSPITIIRAVAPQGSSPGASGQVSGNTIQIITKGGAKSTLNQASAANLLKSLSTSQGLTTPLTITTTANSSGVTTKLVTIPISALTGAATTSVSPAINAIMSTTAQVVSNTAAVQKTITTPVALSTVGTAQTTLISTVGTTPTVSLPSTDQQKVAEDASETKPTAAEESKENKEAQSTTDTTATPTPTTQEAATPATTEASEGGVTIKKEEQTSEPNQQPKVESAASEATPMETDPSPAPGTGIPAEPTDTTKTNGSAPAEETKNKALDNGAVGEEPMDTKESNPDPDSDPKSIAMTIASLANSAGPPVMSTRPITNGLDSKTEDGKSSIGQAIQLAKKDVNQWYDVGIIKTTTTTVAHYHLPSEIISRNEDDIDVVSVPDHSFLKKQELHPGTAYKFRVAGINASGRGPWSEISAFKTCLPGFPGAPSAIRISKSPDGAHLSWEPPANTNGEIQEYSVYLAVRSTMSGASGDAKPTTPAQLAFVRVYCGPNPSCVVSSANLAAAHIDCTSKPAIIFRIAARNVKGYGPATQVRWLQDNQTAAKPTTTVAAKRPASSIESPMVKKVKAENDSDSKTA

Target ORF information:

RefSeq Version	XM_031000420.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031000420.1

ORF Insert Sequence:

ATGGCGGCTC CCATACTAAA ATGGAAGCGT GTGACAAATA CGACTGGTCC AAGCCCCCGA 
CCTCGCCATG GACATCGAGC CGTCGCAATC AAAGACCTTA TGGTAGTCTT CGGCGGTGGG 
AATGAGGGGA TCGTGGATGA ACTTCATGTT TACAATACAG CTACCAACCA GTGGTTTGTC 
CCAGCAGTGA GAGGGGATAT ACCCCCAGGC TGTGCAGCTT ATGGCTTTGT CAGTGATGGC 
ACCCGTCTCT TCATATTTGG AGGAATGGTG GAGTATGGGA AGTACTCCAA CGAGCTATAT 
GAACTGCAAG CCAGTAGATG GGAATGGAAA CGACTCAAGC CTAAGACTGC TAAGAATGCA 
CCACCCCCAT GCCCTCGACT TGGTCACACG TTCACCATGC TTGGGAGTAA AGCATATCTC 
TTTGGAGGAC TTGCCAATGA TAGTGATGAT CCAAAGAATA ATATACCAAG GTATCTCAAT 
GATCTCTACT CACTGGAGCT GAGATCAGGC TACTCGAACA TGATATGGGA CATACCCCTA 
ACCACAGGAC CTGCCCCTCC ACCTAGAGAA TCACATACCG TCGTAGGTTA TGCTCCAAAG 
GATGGCTCCT TTAATCGACT CATTGTATAT GGTGGTATGA GCGGTTGTCG GCTCGGTGAC 
CTTTGGCAAC TCAACATGGA TACACATACA TGGATCAAAC CTGAACTCAA TGGACCCAAC 
CCACTACCAA GAAGCTTGCA CTCTGCCACT GTCATCGGAA ATAGAATGTT TGTGTTTGGT 
GGCTGGGTAC CTCTTGTGAT GGATGATGTC AAAGTAGCAG CTCATGAAAA GGAATGGAAG 
TGTACAAACA CACTGGCTTC TCTCAATCTA GCAACCCACA CATGGGAGCC ACTGGCGATG 
GAGGTGTTTG AAGAGGCTGT ACCTAGAGCC AGAGCTGGGC ATTGCTCTGT TGCTATCCAT 
ACCAGACTCT ACGTATGGAG TGGAAGGGAC GGATATCGCA AGGCTTGGAA TAACCAGGTG 
TGCTGCAAAG ACCTTTGGTT CTTGGAGACT GAGAAGCCCA TGGCCCCAGG CAGGGTCCAG 
CTAGTCCGAG CCAGCACCAC CACGCTGGAA GTATCATGGG GTGCTGTAGC CACAGCCGAT 
GCCTATTTAC TACAGCTGCA GAAGTATGAC ATGCCCCCTA CCTCAAGTGC AGCCTTATCC 
TCTGTGGGTG GCGCTGCTTC CACCAAGCCA CTCCAGTCAC CAATCAAAGC ACAAGCAACC 
ACCACGTTCC GTGTGGTGGC TCCAGGCTCC TCAGCCTCTT GCCCTATTCA GTTGATAAAA 
CACACTCCAG GAGTTAAGAT TCAGAGTCCA ACCTCGATGG CTGGCTCAGC AGCCAGTTCA 
CTCTTTAAAC TCAGCAAGCC CCTCCCTACT CCCGGCGTCC AGCAGCAGAA CACCCTGAGC 
GGTATCGTAG CTCTCGCTGA GGCTGCTGCA GGCACCCAGA AGATGGTCAG TAGTACCACC 
GCTGTCTCAC CAACCCCCAA CAAACCGCTC CTGGCAACGG TAGGAGGGTC CAACCTGACC 
CTGGTGACCC GGTCCATGCC AGGAGGTGTC AGCGTCACAT TGCCGCAAGG GATGAAGCTC 
ACCACGGCAG CAGGAGGCGG GCTCAAGATG ACGACGGCTG GAGGCAAACC CCAATTGGTG 
TCAGCTATGG TTTCAGGCAC CACTAAAACT GTTACACTTG TGCAAAACGC TGGTGCTAAC 
AAGGCAGGTG GTGTAGGGCC TGGCACTACC ATCGTAAAAC TTGTCTCAAC CACCCAGGCT 
GGAGGCAAGC CTACTACCAT CATCACCCCT ATCAGCCAGG CGGGGTTGAA AGGTCAACCT 
ACGATCCTGG GTATCAGCAG CATCACGGCA GGTCAGCAAG GCAAAAGCAT CATCAAGACC 
ATTCCAGTTG GTAAAGGTGT CGCCAACACC GCAACCGGCA AGACGCCCAC CATCGTCACT 
GTAACCAACA AGATGCTGGC AGGAGCCACG GGTACTCCCA CCAAGTTTAT CACGACTGGA 
GGGGTTGGTA AGCAGCAGAA GATTGTCTTG ACAACCCAAG CAGGCGCCTC CCCTACCATC 
CTATCAGGAA CACTCAAAGG TCTAGGGGCA GGGAATTCCC CCATCACCAT CATCCGTGCT 
GTAGCGCCCC AGGGTTCGTC TCCCGGAGCC AGCGGTCAGG TCTCAGGTAA CACCATACAG 
ATCATTACCA AGGGTGGGGC TAAGAGTACC CTCAACCAGG CCAGTGCTGC TAACCTACTC 
AAATCACTCT CCACCTCTCA AGGCCTGACT ACGCCACTCA CAATCACCAC CACCGCTAAC 
TCATCGGGAG TCACAACTAA GCTGGTAACC ATTCCCATTT CAGCCCTGAC GGGGGCAGCA 
ACCACCAGCG TTTCCCCTGC CATAAACGCA ATCATGTCTA CAACAGCCCA GGTTGTCTCA 
AACACAGCAG CTGTGCAAAA GACCATCACT ACCCCTGTAG CATTGAGCAC TGTTGGTACA 
GCACAGACTA CTCTCATATC AACGGTAGGA ACAACACCTA CAGTCAGCCT TCCGAGTACA 
GATCAACAGA AGGTGGCTGA AGATGCATCA GAAACCAAAC CTACAGCTGC AGAAGAAAGC 
AAAGAAAACA AAGAAGCACA ATCAACAACA GACACAACGG CAACACCAAC TCCTACAACA 
CAAGAGGCTG CAACCCCTGC CACTACAGAG GCATCAGAGG GCGGTGTAAC GATAAAGAAG 
GAAGAGCAGA CTTCAGAACC CAATCAACAA CCCAAGGTAG AATCGGCTGC TTCAGAAGCC 
ACGCCCATGG AAACCGACCC CTCCCCTGCA CCCGGAACAG GCATACCAGC CGAGCCTACC 
GATACCACCA AGACCAATGG CTCCGCCCCT GCTGAAGAGA CAAAGAACAA GGCCTTGGAT 
AACGGGGCTG TGGGAGAAGA GCCGATGGAT ACGAAGGAGA GCAACCCAGA TCCAGATTCA 
GATCCTAAGT CTATTGCCAT GACGATTGCA TCACTTGCTA ACTCGGCCGG TCCTCCTGTG 
ATGTCGACCA GGCCTATCAC CAATGGACTG GACTCTAAAA CGGAGGATGG CAAGTCATCG 
ATAGGACAAG CCATCCAGCT AGCCAAGAAA GATGTCAACC AGTGGTACGA CGTTGGAATC 
ATTAAGACGA CGACCACTAC AGTAGCGCAT TATCACTTGC CATCGGAGAT CATTAGCAGA 
AATGAAGATG ACATTGATGT TGTCAGTGTG CCGGATCACA GCTTCCTCAA GAAGCAGGAG 
CTTCACCCGG GCACAGCCTA CAAGTTCAGG GTCGCTGGTA TCAATGCTAG CGGGCGGGGC 
CCATGGAGCG AGATCTCCGC GTTCAAGACA TGCTTGCCTG GATTCCCTGG GGCACCTTCA 
GCTATTAGAA TCAGCAAGAG TCCCGATGGA GCTCATCTAT CCTGGGAGCC TCCGGCAAAC 
ACCAACGGTG AGATCCAGGA GTATTCCGTC TACCTTGCCG TACGTAGCAC CATGAGCGGG 
GCGTCGGGAG ATGCCAAGCC CACTACCCCA GCCCAGCTAG CCTTCGTCAG GGTATACTGC 
GGACCCAACC CATCTTGCGT CGTGAGCTCG GCTAACCTGG CCGCAGCTCA CATCGACTGC 
ACCAGCAAAC CTGCTATCAT TTTCAGAATC GCTGCACGTA ACGTCAAGGG ATATGGACCA 
GCCACTCAGG TTAGATGGCT TCAAGATAAC CAGACTGCTG CTAAACCAAC AACTACTGTC 
GCTGCCAAGA GACCAGCATC TTCTATAGAA AGTCCGATGG TGAAGAAAGT AAAAGCAGAA 
AACGACAGTG ATTCCAAGAC GGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC586153 ( XM_031000422.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030856282.1 Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSd66404
Clone ID Related Accession (Same CDS sequence)	XM_031000422.1
Accession Version	XM_031000422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	host cell factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC CCATACTAAA ATGGAAGCGT GTGACAAATA CGACTGGTCC AAGCCCCCGA 
CCTCGCCATG GACATCGAGC CGTCGCAATC AAAGACCTTA TGGTAGTCTT CGGCGGTGGG 
AATGAGGGGA TCGTGGATGA ACTTCATGTT TACAATACAG CTACCAACCA GTGGTTTGTC 
CCAGCAGTGA GAGGGGATAT ACCCCCAGGC TGTGCAGCTT ATGGCTTTGT CAGTGATGGC 
ACCCGTCTCT TCATATTTGG AGGAATGGTG GAGTATGGGA AGTACTCCAA CGAGCTATAT 
GAACTGCAAG CCAGTAGATG GGAATGGAAA CGACTCAAGC CTAAGACTGC TAAGAATGCA 
CCACCCCCAT GCCCTCGACT TGGTCACACG TTCACCATGC TTGGGAGTAA AGCATATCTC 
TTTGGAGGAC TTGCCAATGA TAGTGATGAT CCAAAGAATA ATATACCAAG GTATCTCAAT 
GATCTCTACT CACTGGAGCT GAGATCAGGC TACTCGAACA TGATATGGGA CATACCCCTA 
ACCACAGGAC CTGCCCCTCC ACCTAGAGAA TCACATACCG TCGTAGGTTA TGCTCCAAAG 
GATGGCTCCT TTAATCGACT CATTGTATAT GGTGGTATGA GCGGTTGTCG GCTCGGTGAC 
CTTTGGCAAC TCAACATGGA TACACATACA TGGATCAAAC CTGAACTCAA TGGACCCAAC 
CCACTACCAA GAAGCTTGCA CTCTGCCACT GTCATCGGAA ATAGAATGTT TGTGTTTGGT 
GGCTGGGTAC CTCTTGTGAT GGATGATGTC AAAGTAGCAG CTCATGAAAA GGAATGGAAG 
TGTACAAACA CACTGGCTTC TCTCAATCTA GCAACCCACA CATGGGAGCC ACTGGCGATG 
GAGGTGTTTG AAGAGGCTGT ACCTAGAGCC AGAGCTGGGC ATTGCTCTGT TGCTATCCAT 
ACCAGACTCT ACGTATGGAG TGGAAGGGAC GGATATCGCA AGGCTTGGAA TAACCAGGTG 
TGCTGCAAAG ACCTTTGGTT CTTGGAGACT GAGAAGCCCA TGGCCCCAGG CAGGGTCCAG 
CTAGTCCGAG CCAGCACCAC CACGCTGGAA GTATCATGGG GTGCTGTAGC CACAGCCGAT 
GCCTATTTAC TACAGCTGCA GAAGTATGAC ATGCCCCCTA CCTCAAGTGC AGCCTTATCC 
TCTGTGGGTG GCGCTGCTTC CACCAAGCCA CTCCAGTCAC CAATCAAAGC ACAAGCTCCA 
GGAGTTAAGA TTCAGAGTCC AACCTCGATG GCTGGCTCAG CAGCCAGTTC ACTCTTTAAA 
CTCAGCAAGC CCCTCCCTAC TCCCGGCGTC CAGCAGCAGA ACACCCTGAG CGGTATCGTA 
GCTCTCGCTG AGGCTGCTGC AGGCACCCAG AAGATGGTCA GTAGTACCAC CGCTGTCTCA 
CCAACCCCCA ACAAACCGCT CCTGGCAACG GTAGGAGGGT CCAACCTGAC CCTGGTGACC 
CGGTCCATGC CAGGAGGTGT CAGCGTCACA TTGCCGCAAG GGATGAAGCT CACCACGGCA 
GCAGGAGGCG GGCTCAAGAT GACGACGGCT GGAGGCAAAC CCCAATTGGT GTCAGCTATG 
GTTTCAGGCA CCACTAAAAC TGTTACACTT GTGCAAAACG CTGGTGCTAA CAAGGCAGGT 
GGTGTAGGGC CTGGCACTAC CATCGTAAAA CTTGTCTCAA CCACCCAGGC TGGAGGCAAG 
CCTACTACCA TCATCACCCC TATCAGCCAG GCGGGGTTGA AAGGTCAACC TACGATCCTG 
GGTATCAGCA GCATCACGGC AGGTCAGCAA GGCAAAAGCA TCATCAAGAC CATTCCAGTT 
GGTAAAGCAG GTGTCGCCAA CACCGCAACC GGCAAGACGC CCACCATCGT CACTGTAACC 
AACAAGATGC TGGCAGGAGC CACGGGTACT CCCACCAAGT TTATCACGAC TGGAGGGGTT 
GGTAAGCAGC AGAAGATTGT CTTGACAACC CAAGCAGGCG CCTCCCCTAC CATCCTATCA 
GGAACACTCA AAGGTCTAGG GGCAGGGAAT TCCCCCATCA CCATCATCCG TGCTGTAGCG 
CCCCAGGGTT CGTCTCCCGG AGCCAGCGGT CAGGTCTCAG GTAACACCAT ACAGATCATT 
ACCAAGGGTG GGGCTAAGAG TACCCTCAAC CAGGCCAGTG CTGCTAACCT ACTCAAATCA 
CTCTCCACCT CTCAAGGCCT GACTACGCCA CTCACAATCA CCACCACCGC TAACTCATCG 
GGAGTCACAA CTAAGCTGGT AACCATTCCC ATTTCAGCCC TGACGGGGGC AGCAACCACC 
AGCGTTTCCC CTGCCATAAA CGCAATCATG TCTACAACAG CCCAGGTTGT CTCAAACACA 
GCAGCTGTGC AAAAGACCAT CACTACCCCT GTAGCATTGA GCACTGTTGG TACAGCACAG 
ACTACTCTCA TATCAACGGT AGGAACAACA CCTACAGTCA GCCTTCCGAG TACAGATCAA 
CAGAAGGTGG CTGAAGATGC ATCAGAAACC AAACCTACAG CTGCAGAAGA AAGCAAAGAA 
AACAAAGAAG CACAATCAAC AACAGACACA ACGGCAACAC CAACTCCTAC AACACAAGAG 
GCTGCAACCC CTGCCACTAC AGAGGCATCA GAGGGCGGTG TAACGATAAA GAAGGAAGAG 
CAGACTTCAG AACCCAATCA ACAACCCAAG GTAGAATCGG CTGCTTCAGA AGCCACGCCC 
ATGGAAACCG ACCCCTCCCC TGCACCCGGA ACAGGCATAC CAGCCGAGCC TACCGATACC 
ACCAAGACCA ATGGCTCCGC CCCTGCTGAA GAGACAAAGA ACAAGGCCTT GGATAACGGG 
GCTGTGGGAG AAGAGCCGAT GGATACGAAG GAGAGCAACC CAGATCCAGA TTCAGATCCT 
AAGTCTATTG CCATGACGAT TGCATCACTT GCTAACTCGG CCGGTCCTCC TGTGATGTCG 
ACCAGGCCTA TCACCAATGG ACTGGACTCT AAAACGGAGG ATGGCAAGTC ATCGATAGGA 
CAAGCCATCC AGCTAGCCAA GAAAGATGTC AACCAGTGGT ACGACGTTGG AATCATTAAG 
ACGACGACCA CTACAGTAGC GCATTATCAC TTGCCATCGG AGATCATTAG CAGAAATGAA 
GATGACATTG ATGTTGTCAG TGTGCCGGAT CACAGCTTCC TCAAGAAGCA GGAGCTTCAC 
CCGGGCACAG CCTACAAGTT CAGGGTCGCT GGTATCAATG CTAGCGGGCG GGGCCCATGG 
AGCGAGATCT CCGCGTTCAA GACATGCTTG CCTGGATTCC CTGGGGCACC TTCAGCTATT 
AGAATCAGCA AGAGTCCCGA TGGAGCTCAT CTATCCTGGG AGCCTCCGGC AAACACCAAC 
GGTGAGATCC AGGAGTATTC CGTCTACCTT GCCGTACGTA GCACCATGAG CGGGGCGTCG 
GGAGATGCCA AGCCCACTAC CCCAGCCCAG CTAGCCTTCG TCAGGGTATA CTGCGGACCC 
AACCCATCTT GCGTCGTGAG CTCGGCTAAC CTGGCCGCAG CTCACATCGA CTGCACCAGC 
AAACCTGCTA TCATTTTCAG AATCGCTGCA CGTAACGTCA AGGGATATGG ACCAGCCACT 
CAGGTTAGAT GGCTTCAAGA TAACCAGACT GCTGCTAAAC CAACAACTAC TGTCGCTGCC 
AAGAGACCAG CATCTTCTAT AGAAAGTCCG ATGGTGAAGA AAGTAAAAGC AGAAAACGAC 
AGTGATTCCA AGACGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030856282.1
CDS	29..3829
Translation	MAAPILKWKRVTNTTGPSPRPRHGHRAVAIKDLMVVFGGGNEGIVDELHVYNTATNQWFVPAVRGDIPPGCAAYGFVSDGTRLFIFGGMVEYGKYSNELYELQASRWEWKRLKPKTAKNAPPPCPRLGHTFTMLGSKAYLFGGLANDSDDPKNNIPRYLNDLYSLELRSGYSNMIWDIPLTTGPAPPPRESHTVVGYAPKDGSFNRLIVYGGMSGCRLGDLWQLNMDTHTWIKPELNGPNPLPRSLHSATVIGNRMFVFGGWVPLVMDDVKVAAHEKEWKCTNTLASLNLATHTWEPLAMEVFEEAVPRARAGHCSVAIHTRLYVWSGRDGYRKAWNNQVCCKDLWFLETEKPMAPGRVQLVRASTTTLEVSWGAVATADAYLLQLQKYDMPPTSSAALSSVGGAASTKPLQSPIKAQAPGVKIQSPTSMAGSAASSLFKLSKPLPTPGVQQQNTLSGIVALAEAAAGTQKMVSSTTAVSPTPNKPLLATVGGSNLTLVTRSMPGGVSVTLPQGMKLTTAAGGGLKMTTAGGKPQLVSAMVSGTTKTVTLVQNAGANKAGGVGPGTTIVKLVSTTQAGGKPTTIITPISQAGLKGQPTILGISSITAGQQGKSIIKTIPVGKAGVANTATGKTPTIVTVTNKMLAGATGTPTKFITTGGVGKQQKIVLTTQAGASPTILSGTLKGLGAGNSPITIIRAVAPQGSSPGASGQVSGNTIQIITKGGAKSTLNQASAANLLKSLSTSQGLTTPLTITTTANSSGVTTKLVTIPISALTGAATTSVSPAINAIMSTTAQVVSNTAAVQKTITTPVALSTVGTAQTTLISTVGTTPTVSLPSTDQQKVAEDASETKPTAAEESKENKEAQSTTDTTATPTPTTQEAATPATTEASEGGVTIKKEEQTSEPNQQPKVESAASEATPMETDPSPAPGTGIPAEPTDTTKTNGSAPAEETKNKALDNGAVGEEPMDTKESNPDPDSDPKSIAMTIASLANSAGPPVMSTRPITNGLDSKTEDGKSSIGQAIQLAKKDVNQWYDVGIIKTTTTTVAHYHLPSEIISRNEDDIDVVSVPDHSFLKKQELHPGTAYKFRVAGINASGRGPWSEISAFKTCLPGFPGAPSAIRISKSPDGAHLSWEPPANTNGEIQEYSVYLAVRSTMSGASGDAKPTTPAQLAFVRVYCGPNPSCVVSSANLAAAHIDCTSKPAIIFRIAARNVKGYGPATQVRWLQDNQTAAKPTTTVAAKRPASSIESPMVKKVKAENDSDSKTA

Target ORF information:

RefSeq Version	XM_031000422.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031000422.1

ORF Insert Sequence:

ATGGCGGCTC CCATACTAAA ATGGAAGCGT GTGACAAATA CGACTGGTCC AAGCCCCCGA 
CCTCGCCATG GACATCGAGC CGTCGCAATC AAAGACCTTA TGGTAGTCTT CGGCGGTGGG 
AATGAGGGGA TCGTGGATGA ACTTCATGTT TACAATACAG CTACCAACCA GTGGTTTGTC 
CCAGCAGTGA GAGGGGATAT ACCCCCAGGC TGTGCAGCTT ATGGCTTTGT CAGTGATGGC 
ACCCGTCTCT TCATATTTGG AGGAATGGTG GAGTATGGGA AGTACTCCAA CGAGCTATAT 
GAACTGCAAG CCAGTAGATG GGAATGGAAA CGACTCAAGC CTAAGACTGC TAAGAATGCA 
CCACCCCCAT GCCCTCGACT TGGTCACACG TTCACCATGC TTGGGAGTAA AGCATATCTC 
TTTGGAGGAC TTGCCAATGA TAGTGATGAT CCAAAGAATA ATATACCAAG GTATCTCAAT 
GATCTCTACT CACTGGAGCT GAGATCAGGC TACTCGAACA TGATATGGGA CATACCCCTA 
ACCACAGGAC CTGCCCCTCC ACCTAGAGAA TCACATACCG TCGTAGGTTA TGCTCCAAAG 
GATGGCTCCT TTAATCGACT CATTGTATAT GGTGGTATGA GCGGTTGTCG GCTCGGTGAC 
CTTTGGCAAC TCAACATGGA TACACATACA TGGATCAAAC CTGAACTCAA TGGACCCAAC 
CCACTACCAA GAAGCTTGCA CTCTGCCACT GTCATCGGAA ATAGAATGTT TGTGTTTGGT 
GGCTGGGTAC CTCTTGTGAT GGATGATGTC AAAGTAGCAG CTCATGAAAA GGAATGGAAG 
TGTACAAACA CACTGGCTTC TCTCAATCTA GCAACCCACA CATGGGAGCC ACTGGCGATG 
GAGGTGTTTG AAGAGGCTGT ACCTAGAGCC AGAGCTGGGC ATTGCTCTGT TGCTATCCAT 
ACCAGACTCT ACGTATGGAG TGGAAGGGAC GGATATCGCA AGGCTTGGAA TAACCAGGTG 
TGCTGCAAAG ACCTTTGGTT CTTGGAGACT GAGAAGCCCA TGGCCCCAGG CAGGGTCCAG 
CTAGTCCGAG CCAGCACCAC CACGCTGGAA GTATCATGGG GTGCTGTAGC CACAGCCGAT 
GCCTATTTAC TACAGCTGCA GAAGTATGAC ATGCCCCCTA CCTCAAGTGC AGCCTTATCC 
TCTGTGGGTG GCGCTGCTTC CACCAAGCCA CTCCAGTCAC CAATCAAAGC ACAAGCTCCA 
GGAGTTAAGA TTCAGAGTCC AACCTCGATG GCTGGCTCAG CAGCCAGTTC ACTCTTTAAA 
CTCAGCAAGC CCCTCCCTAC TCCCGGCGTC CAGCAGCAGA ACACCCTGAG CGGTATCGTA 
GCTCTCGCTG AGGCTGCTGC AGGCACCCAG AAGATGGTCA GTAGTACCAC CGCTGTCTCA 
CCAACCCCCA ACAAACCGCT CCTGGCAACG GTAGGAGGGT CCAACCTGAC CCTGGTGACC 
CGGTCCATGC CAGGAGGTGT CAGCGTCACA TTGCCGCAAG GGATGAAGCT CACCACGGCA 
GCAGGAGGCG GGCTCAAGAT GACGACGGCT GGAGGCAAAC CCCAATTGGT GTCAGCTATG 
GTTTCAGGCA CCACTAAAAC TGTTACACTT GTGCAAAACG CTGGTGCTAA CAAGGCAGGT 
GGTGTAGGGC CTGGCACTAC CATCGTAAAA CTTGTCTCAA CCACCCAGGC TGGAGGCAAG 
CCTACTACCA TCATCACCCC TATCAGCCAG GCGGGGTTGA AAGGTCAACC TACGATCCTG 
GGTATCAGCA GCATCACGGC AGGTCAGCAA GGCAAAAGCA TCATCAAGAC CATTCCAGTT 
GGTAAAGCAG GTGTCGCCAA CACCGCAACC GGCAAGACGC CCACCATCGT CACTGTAACC 
AACAAGATGC TGGCAGGAGC CACGGGTACT CCCACCAAGT TTATCACGAC TGGAGGGGTT 
GGTAAGCAGC AGAAGATTGT CTTGACAACC CAAGCAGGCG CCTCCCCTAC CATCCTATCA 
GGAACACTCA AAGGTCTAGG GGCAGGGAAT TCCCCCATCA CCATCATCCG TGCTGTAGCG 
CCCCAGGGTT CGTCTCCCGG AGCCAGCGGT CAGGTCTCAG GTAACACCAT ACAGATCATT 
ACCAAGGGTG GGGCTAAGAG TACCCTCAAC CAGGCCAGTG CTGCTAACCT ACTCAAATCA 
CTCTCCACCT CTCAAGGCCT GACTACGCCA CTCACAATCA CCACCACCGC TAACTCATCG 
GGAGTCACAA CTAAGCTGGT AACCATTCCC ATTTCAGCCC TGACGGGGGC AGCAACCACC 
AGCGTTTCCC CTGCCATAAA CGCAATCATG TCTACAACAG CCCAGGTTGT CTCAAACACA 
GCAGCTGTGC AAAAGACCAT CACTACCCCT GTAGCATTGA GCACTGTTGG TACAGCACAG 
ACTACTCTCA TATCAACGGT AGGAACAACA CCTACAGTCA GCCTTCCGAG TACAGATCAA 
CAGAAGGTGG CTGAAGATGC ATCAGAAACC AAACCTACAG CTGCAGAAGA AAGCAAAGAA 
AACAAAGAAG CACAATCAAC AACAGACACA ACGGCAACAC CAACTCCTAC AACACAAGAG 
GCTGCAACCC CTGCCACTAC AGAGGCATCA GAGGGCGGTG TAACGATAAA GAAGGAAGAG 
CAGACTTCAG AACCCAATCA ACAACCCAAG GTAGAATCGG CTGCTTCAGA AGCCACGCCC 
ATGGAAACCG ACCCCTCCCC TGCACCCGGA ACAGGCATAC CAGCCGAGCC TACCGATACC 
ACCAAGACCA ATGGCTCCGC CCCTGCTGAA GAGACAAAGA ACAAGGCCTT GGATAACGGG 
GCTGTGGGAG AAGAGCCGAT GGATACGAAG GAGAGCAACC CAGATCCAGA TTCAGATCCT 
AAGTCTATTG CCATGACGAT TGCATCACTT GCTAACTCGG CCGGTCCTCC TGTGATGTCG 
ACCAGGCCTA TCACCAATGG ACTGGACTCT AAAACGGAGG ATGGCAAGTC ATCGATAGGA 
CAAGCCATCC AGCTAGCCAA GAAAGATGTC AACCAGTGGT ACGACGTTGG AATCATTAAG 
ACGACGACCA CTACAGTAGC GCATTATCAC TTGCCATCGG AGATCATTAG CAGAAATGAA 
GATGACATTG ATGTTGTCAG TGTGCCGGAT CACAGCTTCC TCAAGAAGCA GGAGCTTCAC 
CCGGGCACAG CCTACAAGTT CAGGGTCGCT GGTATCAATG CTAGCGGGCG GGGCCCATGG 
AGCGAGATCT CCGCGTTCAA GACATGCTTG CCTGGATTCC CTGGGGCACC TTCAGCTATT 
AGAATCAGCA AGAGTCCCGA TGGAGCTCAT CTATCCTGGG AGCCTCCGGC AAACACCAAC 
GGTGAGATCC AGGAGTATTC CGTCTACCTT GCCGTACGTA GCACCATGAG CGGGGCGTCG 
GGAGATGCCA AGCCCACTAC CCCAGCCCAG CTAGCCTTCG TCAGGGTATA CTGCGGACCC 
AACCCATCTT GCGTCGTGAG CTCGGCTAAC CTGGCCGCAG CTCACATCGA CTGCACCAGC 
AAACCTGCTA TCATTTTCAG AATCGCTGCA CGTAACGTCA AGGGATATGG ACCAGCCACT 
CAGGTTAGAT GGCTTCAAGA TAACCAGACT GCTGCTAAAC CAACAACTAC TGTCGCTGCC 
AAGAGACCAG CATCTTCTAT AGAAAGTCCG ATGGTGAAGA AAGTAAAAGC AGAAAACGAC 
AGTGATTCCA AGACGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC586153 ( XM_031000423.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030856283.1 Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSd66405
Clone ID Related Accession (Same CDS sequence)	XM_031000423.1
Accession Version	XM_031000423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	host cell factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC CCATACTAAA ATGGAAGCGT GTGACAAATA CGACTGGTCC AAGCCCCCGA 
CCTCGCCATG GACATCGAGC CGTCGCAATC AAAGACCTTA TGGTAGTCTT CGGCGGTGGG 
AATGAGGGGA TCGTGGATGA ACTTCATGTT TACAATACAG CTACCAACCA GTGGTTTGTC 
CCAGCAGTGA GAGGGGATAT ACCCCCAGGC TGTGCAGCTT ATGGCTTTGT CAGTGATGGC 
ACCCGTCTCT TCATATTTGG AGGAATGGTG GAGTATGGGA AGTACTCCAA CGAGCTATAT 
GAACTGCAAG CCAGTAGATG GGAATGGAAA CGACTCAAGC CTAAGACTGC TAAGAATGCA 
CCACCCCCAT GCCCTCGACT TGGTCACACG TTCACCATGC TTGGGAGTAA AGCATATCTC 
TTTGGAGGAC TTGCCAATGA TAGTGATGAT CCAAAGAATA ATATACCAAG GTATCTCAAT 
GATCTCTACT CACTGGAGCT GAGATCAGGC TACTCGAACA TGATATGGGA CATACCCCTA 
ACCACAGGAC CTGCCCCTCC ACCTAGAGAA TCACATACCG TCGTAGGTTA TGCTCCAAAG 
GATGGCTCCT TTAATCGACT CATTGTATAT GGTGGTATGA GCGGTTGTCG GCTCGGTGAC 
CTTTGGCAAC TCAACATGGA TACACATACA TGGATCAAAC CTGAACTCAA TGGACCCAAC 
CCACTACCAA GAAGCTTGCA CTCTGCCACT GTCATCGGAA ATAGAATGTT TGTGTTTGGT 
GGCTGGGTAC CTCTTGTGAT GGATGATGTC AAAGTAGCAG CTCATGAAAA GGAATGGAAG 
TGTACAAACA CACTGGCTTC TCTCAATCTA GCAACCCACA CATGGGAGCC ACTGGCGATG 
GAGGTGTTTG AAGAGGCTGT ACCTAGAGCC AGAGCTGGGC ATTGCTCTGT TGCTATCCAT 
ACCAGACTCT ACGTATGGAG TGGAAGGGAC GGATATCGCA AGGCTTGGAA TAACCAGGTG 
TGCTGCAAAG ACCTTTGGTT CTTGGAGACT GAGAAGCCCA TGGCCCCAGG CAGGGTCCAG 
CTAGTCCGAG CCAGCACCAC CACGCTGGAA GTATCATGGG GTGCTGTAGC CACAGCCGAT 
GCCTATTTAC TACAGCTGCA GAAGTATGAC ATGCCCCCTA CCTCAAGTGC AGCCTTATCC 
TCTGTGGGTG GCGCTGCTTC CACCAAGCCA CTCCAGTCAC CAATCAAAGC ACAAGCAACC 
ACCACGTTCC GTGTGGTGGC TCCAGGCTCC TCAGCCTCTT GCCCTATTCA GTTGATAAAA 
CACACTCCAG GAGTTAAGAT TCAGAGTCCA ACCTCGATGG CTGGCTCAGC AGCCAGTTCA 
CTCTTTAAAC TCAGCAAGCC CCTCCCTACT CCCGGCGTCC AGCAGCAGAA CACCCTGAGC 
GGTATCGTAG CTCTCGCTGA GGCTGCTGCA GGCACCCAGA AGATGGTCAG TAGTACCACC 
GCTGTCTCAC CAACCCCCAA CAAACCGCTC CTGGCAACGG TAGGAGGGTC CAACCTGACC 
CTGGTGACCC GGTCCATGCC AGGAGGTGTC AGCGTCACAT TGCCGCAAGG GATGAAGCTC 
ACCACGGCAG CAGGAGGCGG GCTCAAGATG ACGACGGCTG GAGGCAAACC CCAATTGGTG 
TCAGCTATGG TTTCAGGCAC CACTAAAACT GTTACACTTG TGCAAAACGC TGGTGCTAAC 
AAGGCAGGTG GTGTAGGGCC TGGCACTACC ATCGTAAAAC TTGTCTCAAC CACCCAGGCT 
GGAGGCAAGC CTACTACCAT CATCACCCCT ATCAGCCAGG CGGGGTTGAA AGGTCAACCT 
ACGATCCTGG GTATCAGCAG CATCACGGCA GGTCAGCAAG GCAAAAGCAT CATCAAGACC 
ATTCCAGTTG GTAAAGCAGG TGTCGCCAAC ACCGCAACCG GCAAGACGCC CACCATCGTC 
ACTGTAACCA ACAAGATGCT GGCAGGAGCC ACGGGTACTC CCACCAAGTT TATCACGACT 
GGAGGGGTTG GTAAGCAGCA GAAGATTGTC TTGACAACCC AAGCAGGCGC CTCCCCTACC 
ATCCTATCAG GAACACTCAA AGGTCTAGGG GCAGGGAATT CCCCCATCAC CATCATCCGT 
GCTGTAGCGC CCCAGGGTTC GTCTCCCGGA GCCAGCGGTC AGGTCTCAGG AGTCACAACT 
AAGCTGGTAA CCATTCCCAT TTCAGCCCTG ACGGGGGCAG CAACCACCAG CGTTTCCCCT 
GCCATAAACG CAATCATGTC TACAACAGCC CAGGTTGTCT CAAACACAGC AGCTGTGCAA 
AAGACCATCA CTACCCCTGT AGCATTGAGC ACTGTTGGTA CAGCACAGAC TACTCTCATA 
TCAACGGTAG GAACAACACC TACAGTCAGC CTTCCGAGTA CAGATCAACA GAAGGTGGCT 
GAAGATGCAT CAGAAACCAA ACCTACAGCT GCAGAAGAAA GCAAAGAAAA CAAAGAAGCA 
CAATCAACAA CAGACACAAC GGCAACACCA ACTCCTACAA CACAAGAGGC TGCAACCCCT 
GCCACTACAG AGGCATCAGA GGGCGGTGTA ACGATAAAGA AGGAAGAGCA GACTTCAGAA 
CCCAATCAAC AACCCAAGGT AGAATCGGCT GCTTCAGAAG CCACGCCCAT GGAAACCGAC 
CCCTCCCCTG CACCCGGAAC AGGCATACCA GCCGAGCCTA CCGATACCAC CAAGACCAAT 
GGCTCCGCCC CTGCTGAAGA GACAAAGAAC AAGGCCTTGG ATAACGGGGC TGTGGGAGAA 
GAGCCGATGG ATACGAAGGA GAGCAACCCA GATCCAGATT CAGATCCTAA GTCTATTGCC 
ATGACGATTG CATCACTTGC TAACTCGGCC GGTCCTCCTG TGATGTCGAC CAGGCCTATC 
ACCAATGGAC TGGACTCTAA AACGGAGGAT GGCAAGTCAT CGATAGGACA AGCCATCCAG 
CTAGCCAAGA AAGATGTCAA CCAGTGGTAC GACGTTGGAA TCATTAAGAC GACGACCACT 
ACAGTAGCGC ATTATCACTT GCCATCGGAG ATCATTAGCA GAAATGAAGA TGACATTGAT 
GTTGTCAGTG TGCCGGATCA CAGCTTCCTC AAGAAGCAGG AGCTTCACCC GGGCACAGCC 
TACAAGTTCA GGGTCGCTGG TATCAATGCT AGCGGGCGGG GCCCATGGAG CGAGATCTCC 
GCGTTCAAGA CATGCTTGCC TGGATTCCCT GGGGCACCTT CAGCTATTAG AATCAGCAAG 
AGTCCCGATG GAGCTCATCT ATCCTGGGAG CCTCCGGCAA ACACCAACGG TGAGATCCAG 
GAGTATTCCG TCTACCTTGC CGTACGTAGC ACCATGAGCG GGGCGTCGGG AGATGCCAAG 
CCCACTACCC CAGCCCAGCT AGCCTTCGTC AGGGTATACT GCGGACCCAA CCCATCTTGC 
GTCGTGAGCT CGGCTAACCT GGCCGCAGCT CACATCGACT GCACCAGCAA ACCTGCTATC 
ATTTTCAGAA TCGCTGCACG TAACGTCAAG GGATATGGAC CAGCCACTCA GGTTAGATGG 
CTTCAAGATA ACCAGACTGC TGCTAAACCA ACAACTACTG TCGCTGCCAA GAGACCAGCA 
TCTTCTATAG AAAGTCCGAT GGTGAAGAAA GTAAAAGCAG AAAACGACAG TGATTCCAAG 
ACGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030856283.1
CDS	29..3757
Translation	MAAPILKWKRVTNTTGPSPRPRHGHRAVAIKDLMVVFGGGNEGIVDELHVYNTATNQWFVPAVRGDIPPGCAAYGFVSDGTRLFIFGGMVEYGKYSNELYELQASRWEWKRLKPKTAKNAPPPCPRLGHTFTMLGSKAYLFGGLANDSDDPKNNIPRYLNDLYSLELRSGYSNMIWDIPLTTGPAPPPRESHTVVGYAPKDGSFNRLIVYGGMSGCRLGDLWQLNMDTHTWIKPELNGPNPLPRSLHSATVIGNRMFVFGGWVPLVMDDVKVAAHEKEWKCTNTLASLNLATHTWEPLAMEVFEEAVPRARAGHCSVAIHTRLYVWSGRDGYRKAWNNQVCCKDLWFLETEKPMAPGRVQLVRASTTTLEVSWGAVATADAYLLQLQKYDMPPTSSAALSSVGGAASTKPLQSPIKAQATTTFRVVAPGSSASCPIQLIKHTPGVKIQSPTSMAGSAASSLFKLSKPLPTPGVQQQNTLSGIVALAEAAAGTQKMVSSTTAVSPTPNKPLLATVGGSNLTLVTRSMPGGVSVTLPQGMKLTTAAGGGLKMTTAGGKPQLVSAMVSGTTKTVTLVQNAGANKAGGVGPGTTIVKLVSTTQAGGKPTTIITPISQAGLKGQPTILGISSITAGQQGKSIIKTIPVGKAGVANTATGKTPTIVTVTNKMLAGATGTPTKFITTGGVGKQQKIVLTTQAGASPTILSGTLKGLGAGNSPITIIRAVAPQGSSPGASGQVSGVTTKLVTIPISALTGAATTSVSPAINAIMSTTAQVVSNTAAVQKTITTPVALSTVGTAQTTLISTVGTTPTVSLPSTDQQKVAEDASETKPTAAEESKENKEAQSTTDTTATPTPTTQEAATPATTEASEGGVTIKKEEQTSEPNQQPKVESAASEATPMETDPSPAPGTGIPAEPTDTTKTNGSAPAEETKNKALDNGAVGEEPMDTKESNPDPDSDPKSIAMTIASLANSAGPPVMSTRPITNGLDSKTEDGKSSIGQAIQLAKKDVNQWYDVGIIKTTTTTVAHYHLPSEIISRNEDDIDVVSVPDHSFLKKQELHPGTAYKFRVAGINASGRGPWSEISAFKTCLPGFPGAPSAIRISKSPDGAHLSWEPPANTNGEIQEYSVYLAVRSTMSGASGDAKPTTPAQLAFVRVYCGPNPSCVVSSANLAAAHIDCTSKPAIIFRIAARNVKGYGPATQVRWLQDNQTAAKPTTTVAAKRPASSIESPMVKKVKAENDSDSKTA

Target ORF information:

RefSeq Version	XM_031000423.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus host cell factor 2 (LOC586153), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031000423.1

ORF Insert Sequence:

ATGGCGGCTC CCATACTAAA ATGGAAGCGT GTGACAAATA CGACTGGTCC AAGCCCCCGA 
CCTCGCCATG GACATCGAGC CGTCGCAATC AAAGACCTTA TGGTAGTCTT CGGCGGTGGG 
AATGAGGGGA TCGTGGATGA ACTTCATGTT TACAATACAG CTACCAACCA GTGGTTTGTC 
CCAGCAGTGA GAGGGGATAT ACCCCCAGGC TGTGCAGCTT ATGGCTTTGT CAGTGATGGC 
ACCCGTCTCT TCATATTTGG AGGAATGGTG GAGTATGGGA AGTACTCCAA CGAGCTATAT 
GAACTGCAAG CCAGTAGATG GGAATGGAAA CGACTCAAGC CTAAGACTGC TAAGAATGCA 
CCACCCCCAT GCCCTCGACT TGGTCACACG TTCACCATGC TTGGGAGTAA AGCATATCTC 
TTTGGAGGAC TTGCCAATGA TAGTGATGAT CCAAAGAATA ATATACCAAG GTATCTCAAT 
GATCTCTACT CACTGGAGCT GAGATCAGGC TACTCGAACA TGATATGGGA CATACCCCTA 
ACCACAGGAC CTGCCCCTCC ACCTAGAGAA TCACATACCG TCGTAGGTTA TGCTCCAAAG 
GATGGCTCCT TTAATCGACT CATTGTATAT GGTGGTATGA GCGGTTGTCG GCTCGGTGAC 
CTTTGGCAAC TCAACATGGA TACACATACA TGGATCAAAC CTGAACTCAA TGGACCCAAC 
CCACTACCAA GAAGCTTGCA CTCTGCCACT GTCATCGGAA ATAGAATGTT TGTGTTTGGT 
GGCTGGGTAC CTCTTGTGAT GGATGATGTC AAAGTAGCAG CTCATGAAAA GGAATGGAAG 
TGTACAAACA CACTGGCTTC TCTCAATCTA GCAACCCACA CATGGGAGCC ACTGGCGATG 
GAGGTGTTTG AAGAGGCTGT ACCTAGAGCC AGAGCTGGGC ATTGCTCTGT TGCTATCCAT 
ACCAGACTCT ACGTATGGAG TGGAAGGGAC GGATATCGCA AGGCTTGGAA TAACCAGGTG 
TGCTGCAAAG ACCTTTGGTT CTTGGAGACT GAGAAGCCCA TGGCCCCAGG CAGGGTCCAG 
CTAGTCCGAG CCAGCACCAC CACGCTGGAA GTATCATGGG GTGCTGTAGC CACAGCCGAT 
GCCTATTTAC TACAGCTGCA GAAGTATGAC ATGCCCCCTA CCTCAAGTGC AGCCTTATCC 
TCTGTGGGTG GCGCTGCTTC CACCAAGCCA CTCCAGTCAC CAATCAAAGC ACAAGCAACC 
ACCACGTTCC GTGTGGTGGC TCCAGGCTCC TCAGCCTCTT GCCCTATTCA GTTGATAAAA 
CACACTCCAG GAGTTAAGAT TCAGAGTCCA ACCTCGATGG CTGGCTCAGC AGCCAGTTCA 
CTCTTTAAAC TCAGCAAGCC CCTCCCTACT CCCGGCGTCC AGCAGCAGAA CACCCTGAGC 
GGTATCGTAG CTCTCGCTGA GGCTGCTGCA GGCACCCAGA AGATGGTCAG TAGTACCACC 
GCTGTCTCAC CAACCCCCAA CAAACCGCTC CTGGCAACGG TAGGAGGGTC CAACCTGACC 
CTGGTGACCC GGTCCATGCC AGGAGGTGTC AGCGTCACAT TGCCGCAAGG GATGAAGCTC 
ACCACGGCAG CAGGAGGCGG GCTCAAGATG ACGACGGCTG GAGGCAAACC CCAATTGGTG 
TCAGCTATGG TTTCAGGCAC CACTAAAACT GTTACACTTG TGCAAAACGC TGGTGCTAAC 
AAGGCAGGTG GTGTAGGGCC TGGCACTACC ATCGTAAAAC TTGTCTCAAC CACCCAGGCT 
GGAGGCAAGC CTACTACCAT CATCACCCCT ATCAGCCAGG CGGGGTTGAA AGGTCAACCT 
ACGATCCTGG GTATCAGCAG CATCACGGCA GGTCAGCAAG GCAAAAGCAT CATCAAGACC 
ATTCCAGTTG GTAAAGCAGG TGTCGCCAAC ACCGCAACCG GCAAGACGCC CACCATCGTC 
ACTGTAACCA ACAAGATGCT GGCAGGAGCC ACGGGTACTC CCACCAAGTT TATCACGACT 
GGAGGGGTTG GTAAGCAGCA GAAGATTGTC TTGACAACCC AAGCAGGCGC CTCCCCTACC 
ATCCTATCAG GAACACTCAA AGGTCTAGGG GCAGGGAATT CCCCCATCAC CATCATCCGT 
GCTGTAGCGC CCCAGGGTTC GTCTCCCGGA GCCAGCGGTC AGGTCTCAGG AGTCACAACT 
AAGCTGGTAA CCATTCCCAT TTCAGCCCTG ACGGGGGCAG CAACCACCAG CGTTTCCCCT 
GCCATAAACG CAATCATGTC TACAACAGCC CAGGTTGTCT CAAACACAGC AGCTGTGCAA 
AAGACCATCA CTACCCCTGT AGCATTGAGC ACTGTTGGTA CAGCACAGAC TACTCTCATA 
TCAACGGTAG GAACAACACC TACAGTCAGC CTTCCGAGTA CAGATCAACA GAAGGTGGCT 
GAAGATGCAT CAGAAACCAA ACCTACAGCT GCAGAAGAAA GCAAAGAAAA CAAAGAAGCA 
CAATCAACAA CAGACACAAC GGCAACACCA ACTCCTACAA CACAAGAGGC TGCAACCCCT 
GCCACTACAG AGGCATCAGA GGGCGGTGTA ACGATAAAGA AGGAAGAGCA GACTTCAGAA 
CCCAATCAAC AACCCAAGGT AGAATCGGCT GCTTCAGAAG CCACGCCCAT GGAAACCGAC 
CCCTCCCCTG CACCCGGAAC AGGCATACCA GCCGAGCCTA CCGATACCAC CAAGACCAAT 
GGCTCCGCCC CTGCTGAAGA GACAAAGAAC AAGGCCTTGG ATAACGGGGC TGTGGGAGAA 
GAGCCGATGG ATACGAAGGA GAGCAACCCA GATCCAGATT CAGATCCTAA GTCTATTGCC 
ATGACGATTG CATCACTTGC TAACTCGGCC GGTCCTCCTG TGATGTCGAC CAGGCCTATC 
ACCAATGGAC TGGACTCTAA AACGGAGGAT GGCAAGTCAT CGATAGGACA AGCCATCCAG 
CTAGCCAAGA AAGATGTCAA CCAGTGGTAC GACGTTGGAA TCATTAAGAC GACGACCACT 
ACAGTAGCGC ATTATCACTT GCCATCGGAG ATCATTAGCA GAAATGAAGA TGACATTGAT 
GTTGTCAGTG TGCCGGATCA CAGCTTCCTC AAGAAGCAGG AGCTTCACCC GGGCACAGCC 
TACAAGTTCA GGGTCGCTGG TATCAATGCT AGCGGGCGGG GCCCATGGAG CGAGATCTCC 
GCGTTCAAGA CATGCTTGCC TGGATTCCCT GGGGCACCTT CAGCTATTAG AATCAGCAAG 
AGTCCCGATG GAGCTCATCT ATCCTGGGAG CCTCCGGCAA ACACCAACGG TGAGATCCAG 
GAGTATTCCG TCTACCTTGC CGTACGTAGC ACCATGAGCG GGGCGTCGGG AGATGCCAAG 
CCCACTACCC CAGCCCAGCT AGCCTTCGTC AGGGTATACT GCGGACCCAA CCCATCTTGC 
GTCGTGAGCT CGGCTAACCT GGCCGCAGCT CACATCGACT GCACCAGCAA ACCTGCTATC 
ATTTTCAGAA TCGCTGCACG TAACGTCAAG GGATATGGAC CAGCCACTCA GGTTAGATGG 
CTTCAAGATA ACCAGACTGC TGCTAAACCA ACAACTACTG TCGCTGCCAA GAGACCAGCA 
TCTTCTATAG AAAGTCCGAT GGTGAAGAAA GTAAAAGCAG AAAACGACAG TGATTCCAAG 
ACGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC586153 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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