mgb cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	mgb
Entrez Gene ID	585663
Full Name	GTP-binding protein
Synonyms	RDZ
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_030989242.1	XP_030845102.1	GTP-binding protein isoform X2
NM_214480.1	NP_999645.1	GTP-binding protein
XM_011676269.1	XP_011674571.1	GTP-binding protein
XM_030989241.1	XP_030845101.1	GTP-binding protein isoform X1
XM_030989243.1	XP_030845103.1	GTP-binding protein isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Tu Q, Cameron RA, Worley KC, Gibbs RA, Davidson EH
Genome research22(10)2079-87(2012 Oct)

Rendezvin: An essential gene encoding independent, differentially secreted egg proteins that organize the fertilization envelope proteome after self-association.
Wong JL, Wessel GM
Molecular biology of the cell17(12)5241-52(2006 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mgb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mgb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd20300	NM_214480.1 Latest version!	Strongylocentrotus purpuratus GTP-binding protein (mgb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OSd55358	XM_030989242.1 Latest version!	Strongylocentrotus purpuratus GTP-binding protein (mgb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSd55359	XM_030989243.1 Latest version!	Strongylocentrotus purpuratus GTP-binding protein (mgb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSd55357	XM_030989241.1 Latest version!	Strongylocentrotus purpuratus GTP-binding protein (mgb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mgb ( NM_214480.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> NP_999645.1 Strongylocentrotus purpuratus GTP-binding protein (mgb), mRNA.

Price & Availability↑

CloneID	OSd20300
Clone ID Related Accession (Same CDS sequence)	NM_214480.1
Accession Version	NM_214480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569600000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	GTP-binding protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF202537.1. On or before May 23, 2007 this sequence version replaced XM_785483.2, XM_001186804.1. ##Evidence-Data-START## Transcript exon combination :: AF202537.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01103190 [ECO:0000348] ##Evidence-Data-END##

ATGGATCGGG AAGGAAATGA GACGATCGCG CTAGAAGAAG AATTTGGTTT CTTTGTGTCG 
CCTGGTTACC CTCTGATCTA CGAGTCGGAC GCGTCGGCGG AATATGCCAT CCGCACCGAC 
CCTAGCAAGC TCCTCGTCGT GACCTTCCAC GACTTCCAGC TGGAGAGAGA CGACTACCCC 
GCTCCGCTGC CTGTGGAGAA TCTTCCCCTG GAGCCTGATA TAATGATAAA GCGAAGCACG 
TTGAACGAGC ATCAGAATCA CTACGGCGAC GATTACGGCG AATGTGTTGA TTACGTCAAG 
ATCATCGACA TTTTCGACAA TCATGTGGAA ACGTACTGTG GGCGGATCCC GGTGTTTAGC 
TATATCTCCC GTGGCAACGA GATCCTGGTC CAGTTTAAGA CCGATCTGAA CATGAACTAC 
AGAGGCTTCA ACGCGTCTTA CAGGTCCATC TCTAGAAGAA CACCAAATGA TTGGGAGCTT 
GTCTACCGAG TGGCAAAAGG AGGCGAAACC GAGGCTCTTG ATAAGTTCCT GAATCAGGAT 
CGGGGCTACA GCGAGTCCGA CCCCAACGCA AGGGGAACCA CAATTACCCG CTCTCTTGCT 
ACCTTCAAGG ATTCAGATGT CCCAAATTGG GCGGATATCG GAATCAGAAA AGTCAAAGTT 
GACGCCTATA AGTCGGGAAG CATCATAGCA ACGTTCCGCT TTGATGGCCA AGGCACCACT 
CTCAGTAACT GGGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999645.1
CDS	1..738
Translation	MDREGNETIALEEEFGFFVSPGYPLIYESDASAEYAIRTDPSKLLVVTFHDFQLERDDYPAPLPVENLPLEPDIMIKRSTLNEHQNHYGDDYGECVDYVKIIDIFDNHVETYCGRIPVFSYISRGNEILVQFKTDLNMNYRGFNASYRSISRRTPNDWELVYRVAKGGETEALDKFLNQDRGYSESDPNARGTTITRSLATFKDSDVPNWADIGIRKVKVDAYKSGSIIATFRFDGQGTTLSNWV

Target ORF information:

RefSeq Version	NM_214480.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus GTP-binding protein (mgb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214480.1

ORF Insert Sequence:

ATGGATCGGG AAGGAAATGA GACGATCGCG CTAGAAGAAG AATTTGGTTT CTTTGTGTCG 
CCTGGTTACC CTCTGATCTA CGAGTCGGAC GCGTCGGCGG AATATGCCAT CCGCACCGAC 
CCTAGCAAGC TCCTCGTCGT GACCTTCCAC GACTTCCAGC TGGAGAGAGA CGACTACCCC 
GCTCCGCTGC CTGTGGAGAA TCTTCCCCTG GAGCCTGATA TAATGATAAA GCGAAGCACG 
TTGAACGAGC ATCAGAATCA CTACGGCGAC GATTACGGCG AATGTGTTGA TTACGTCAAG 
ATCATCGACA TTTTCGACAA TCATGTGGAA ACGTACTGTG GGCGGATCCC GGTGTTTAGC 
TATATCTCCC GTGGCAACGA GATCCTGGTC CAGTTTAAGA CCGATCTGAA CATGAACTAC 
AGAGGCTTCA ACGCGTCTTA CAGGTCCATC TCTAGAAGAA CACCAAATGA TTGGGAGCTT 
GTCTACCGAG TGGCAAAAGG AGGCGAAACC GAGGCTCTTG ATAAGTTCCT GAATCAGGAT 
CGGGGCTACA GCGAGTCCGA CCCCAACGCA AGGGGAACCA CAATTACCCG CTCTCTTGCT 
ACCTTCAAGG ATTCAGATGT CCCAAATTGG GCGGATATCG GAATCAGAAA AGTCAAAGTT 
GACGCCTATA AGTCGGGAAG CATCATAGCA ACGTTCCGCT TTGATGGCCA AGGCACCACT 
CTCAGTAACT GGGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mgb ( XM_030989242.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030845102.1 Strongylocentrotus purpuratus GTP-binding protein (mgb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSd55358
Clone ID Related Accession (Same CDS sequence)	XM_030989242.1
Accession Version	XM_030989242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	GTP-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145603.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACC GTATCAGTGG ACACGCCCTT TGGTTATGCA CTCAGCACGT TTTATTAGTT 
GTCTTATTAC TGGGATGCTG CCATCTACCG TCGGTTGAAT CGGACCCAAC CCTGACGCAT 
AGATGGGCGG TTACATATGG AGAATGTAGT CAGACATGCG GTGGAGGTAC ACAAATCCTT 
GTCAAAAGCT GTTACACATT GGCCACGAAC TCACAGCCCT CACAGACGGC ACCCATTAGC 
TACTGCAGTG GACTGGACCA CCCACCAGAA CCAACCGTTC GATCATGCAG CACACAACCA 
TGCACAATGT GTGATCGTAC TTACACTGCC CTCAGTGGAG CGCTGTCATC ACCATATTGG 
CCTGATAAAT CATCAATACC CAGGTACTGT CGGCTTGTGA TAGATATTGA ACCTGATAAG 
AGGATCTTGT TGGCCTTCCA TGAGTTTACC CTCGAGGGTA TAGATCTGTT TAGTCCCTCC 
AAGACTGAAG AAGATGTCTG CTTGTGTGGT GTGCTCGTGG AGATACGGGA TGATGGTGGG 
CGCATGAATC GTTACTGTGG AACCAAGAAA CCGTTTGCTT GGGTCTCTGA GACGGATACA 
GTAACGCTGG AATTTATCAC ACCCTATTCT CTCGTCTTTA ATCGCTTCCT TGCATCTTAT 
ACCACCATCG ATACAAAACC AAGTTGCTAT CGCTTCATTG ACCTGACCGG CCTCCATACC 
GGTCTCGAGG CAGATGGAAT CACTGAGGTT CCTTTGCCTC AAAAAGGCTT CCTGCACTCG 
TTGAACTTCC CGAACGCCTA TCCCGACAAC CAGAACTGCG AGTACGTTCT CCATGGCAAT 
CCAGAGCATC GCATCGTGCT GTACTTTGAC GAGTTTGAGC TAGAACCTGG TCCGGCATGC 
GAGGCTGACT ATGTCAAGAT TGAGGATTTC TACAGTCGGC GCTCTAACGT CTACTGCGGC 
AAACATCACT CTTTCTCCTG GATCTCTGAT AGCAATGAAG TCTGGATCAC GTTCAGATCC 
AACGCCAATC AAGTCTACAC CGGTTTCCTT CTGGCCTACG AACTCATGGA TCGGGAAGGA 
AATGAGACGA TCGCGCTAGA AGAAGAGTCC GGTTTCTTTG TGTCGCCTGG TTACCCTCTG 
ATCTACGAGT CGGACGCGTC GGCGGAATAC GCCATCCGCA CTGACCCTAG CAAGCTCCTC 
GTCGTGACCT TCCACGACTT CCAGCTGGAG AGAGACGACT ACCCCGCTCC GCTGCCTGTG 
GGGATTCTGG AGATGGACAA GCGAAGCACG TTGAACGAGC ATCAGAATCA CTACGGCGAC 
CGATACGACT TATGTGTTGA TTACGTCAAG ATCATCGACA TTTTCGACAA TCATGTGGAA 
ACGTACTGTG GGCGGATCCC GGTGTTTAGC TATATCTCCC GTGGCAACGA GATCCTGGTC 
CAGTTTAAGA CCGATCTGAA CATGAACTAC AGAGGCTTCA ACGCGTCTTA CAGGTCCATC 
TCTAGAAGAA CACCAAATGA TTGGGAACTT GTCTACCGAG TGGCAAAAGG AGGCGAAACC 
GAGGCTCTTG ATAAGTTCCT GATGCAGGAT CGGGGCTACA GCGAGTCTGA CCCCAATGCA 
AAGGGAACCA CAATTACTCG CTCTCTTGCT ACCTTCAAGG ATTCAGATGT CCCAAATTGG 
GAGAATATCG GAATCAGAAA AGTCAAAGTT GACGCCTATA AGTCGGGAAG CATCATAGCA 
ACTTTCCGCT TTGATGGCCA TGGCACAAGT CTCAGTGACT GGTTTAGTTG CGAGAAGATG 
ACCTACAGCG GTTACGACGA CCTCAACTCA AAAGATTGTG GTGGTCTCGT GTTTGGCCTT 
ACAGGAATGC CGCCGGAGTT TGAAATCACA ACAACAATTG CCAACGGCAA GACGTGCGAT 
GATGACATGG ATCATAGGGT GTGGCTCGAT GTCCAAGATG TTGCGGAAGA GGGGGATTGT 
ACATACGAGG AGCCGGAAAT TGATATCAAC TCCATCATCT TCGTTAGCAG CGCTACGACG 
GGAGGGGAAA CTGCAACGGG AGCAAACATA GCCGAAGCAG ACGTGTTCAC CATCTGGGTT 
CAAAAAGATT GCGATGAAAG AATCTCGCCA GCCAGGGACA GCCCTGTAAA CATTGCTTCC 
AAGGGGCACG ATGAGCTTGA CTACAACAAT AACGAGTATT GCGATCTCTA TCTGAGTGTC 
CATCCGTTTG ATCGGCTCGC GATTGAATTC ACCGATTTTG AGCTGGAAGA AGATGAGTGT 
CTCGCAAATG ATTACGTCCA GATTGAGAAC ATAGAAACGG CCTTTGCGCA GATCTTCTGT 
GGTGTTCAAC ACCAAGTAGT ATGGACCGCT ACTGCGAATG CTGCCATCTT GAGGTTCAGA 
ACGGACAGTG TGGTAACGTA CAATGGTTGG GGTGCCAGTG TGAGAGCAGA ACGGCGAAAT 
TTGGAAGATT GTTCCACCGT CACCGTGAAC CCAACCGGCA ACGTGACCAA TCCCGATTAC 
CCCAACTCAC TCCAGGATTA TCGCTACTAC GCCTGCGAGT TCATCGTACA CGCCCATCCG 
GAGGACCGAG TTATCTTCTC ATTCTCGCGT TTCATCTTTG AGACAGAGAA CCCACCAGAG 
TTGCAGATTG ACCCAAGGTC AACCCGGGCC AACTTCATCG ATGTGTCGGG TCGTCTCTAC 
CCCTTCTCTT GGACCTCTGA TACCAACGAG CTAGCGCTTA TCTACCACGC CCCTCTTCCC 
TACCGGACCT ACATATTCTT TGAGGGCGAC TACCGCACCC TAGAGGCTCC TCGACCGGCC 
ACCTGCCAAC GGTTTGCGGA TGCCGATCTG GGAGGGACGT TCTCCTGGCC GACGGAATCT 
CTGTCGACCG GTCTGCAGAA CTGTTACGTG ATCGTGAGGT CCGACCCAGT CGAGCATAGA 
CGAGTCCAGA TAATCTTTGA CAATCTTAAG ATGCCCCAGT CGGATTGCAA TAGCTACATC 
AATACTCTTG ATACCACTAC AAGGCGTAAC GACATGTATT GTCAGAGAAT CCGCATCCCC 
TTCGCATGGA CCTCAGATGC CAATGAGGTC GTGATTAAAT TTTTCCATGG GCCGACCTTC 
GCTCTCCACC AGGTCAACGT ACTTTACAGG AGCCGAGCAA GGGTTCGTCC TGAAGATGCC 
AAAGTGTTGA CGGCTCTGAC GGGCGGTGAC TTCAGCAGCC CCAACTACCC CCAACCCTAC 
GATTCACAAT CCAAGAAGGA GTACCTCTTG CACGCCCCTG CAGAACATCT CGTCATTGTT 
GTCTTCAAAG ACTTCTCGGT TGAAGGTGCC ATAGAACCAA GTCCTCTTAT AGAGCCTGTA 
TTAAGGCCTG GCCGTAGCGT GGAGGACCTC TCCGCTCACT ACAAACACAT GTACAAGAGG 
CAGACGATAG TAGAGCCACC TACACCTGAA TGCCGCTACG ACTATGTTTC GGTTGAAGAC 
GTACTGGTGA ACACGGAGAC CAAGTACTGC GGGGATCAGC AACCGTTTGG TGTGGCGACT 
CAGGGCAACG AGGCCCTAGT CCTGTTCCTG ACTGATGAGA CCGTTGAGAA ACGAGGATTC 
ACCGCTCTCT ATGCTTTTGA GCCTCGACCG GCTATATCAG ACTGCGGTAC GCTGCAAACC 
GCGGACCAGG GCATCCTCCA GTCTCCGAAC TACCCTAACT ACTACCCGGA CGGGGCATAC 
TGCCGAGACG TGATACAGGT GGACCCAAAG AACCGCATCG TTATCGACTT TCGTTTCTTG 
AACTTGGAGG ATGATATCAC CTGCAGTAAA GACTACCTTG AGGTGCGTGA TGTAGTAACG 
GGGAGAGCGG AGAGGTACTG TGGACACAAG GCTACAGCCT TCCAATGGAA GTCGGACACC 
AACCTGGTCT TCCTGACGTT CGTCAGCGAC AATGCCAACA GCGATCCTGG ATACGATGCA 
GGATACACCA TAGAGGATAG ATCAGATGAA CCTACCTGCG TGCACACCAT CACGGCAACG 
TCCGGTACCA TCCACTCGCC GAGGTTCCCT CGAGACTACC CCAACCAGCT CAGGTGCGAG 
TACAACATCA AGGTGTATCC GGATTATGGA ATCAAGCTCA CCTTCCAAGA GTTCAACCTG 
GAGCCCCCCG CCCCGGGAAG CACGGACTGT GCCTTTGATT ATCTACAGAT TATTGACCCG 
ACGACGGTCA ACGTCGTGAC AGCCGAGAAG CTCTGCGGCT CCAAGAACAC TTTTGAACGT 
CAGATCCAGT TTAATGAAGT CAGTCTCATC TTTGTCAGTG ATTTCATCAC GTTCTACAAG 
GGTTTCCTGG TTGATTTCCA ACAGATCTCG CTCGGACAAA CCCCACAAAC CGTCCAAACT 
ACTAGTTGCA ATGAGCAGCT TACTGCTCCG TCAGGAACCT TCTCTAGCCC TGGTTACCCC 
GGTAACTATC CTGACAACTC CTTGTGCGTC ACCCTCATCA CTGTTGCCGT GGGTTACCAT 
GTCAGAATCA ACTTCAATGA CTTCAATACA GAAGGCTATA CAAGCTGCCC GTATGATTAT 
GTTCTGGTAC AAGATGTTCA GGTTCCTGGT CTGGACGAGA GGTTCTGCGG GCGCAAGCAG 
CACCCTTTCT CGTGGACCTC GGAAAGTAAC GTGGTTTCAG TGACGTTCAA GAGTGACGAT 
AGGACACCCT CGTCCGGTTT CTATGCAGCG TACGAATCAG CGCCGAACGT AGCCGCCGCC 
GTCGTTGCCC CAGTCACAGG TTGTGAGACG CACTACGGCA TCACCGAAAT CAGCGGAACT 
TCAGGAACCG TGCAGTCTCC GGACTATCCT AGCCTCTATC CACTCAATGT TGACTGCTTC 
GTCCGCTTCA CGACCGGTTA CGGATCTCGA TGCCAAATCA ACTTCATCGT CTTCAACCTG 
GAGGCCTCAA GCACATGCAG TTACGACTAT GTAAAGGTAA CGAGTATTCC AGATGGCGCA 
ACCCAGACGT TCTGCGGGTC TCTTGCTCCA TTTGAATGGC ACTCCCATTC TGCTACATGC 
GAGATCCAGT TCCACAGCGA CTCCTACCAA AGAGCATCGG GCTACCATGC ACAAATCACA 
GCTGTTTCTT CAAATATCCA TGCACCCTAC CAATCGCATC AACCTCCAAA TGAACCAGCT 
TCACCGTACC AACCACCAAA TGAACCTCCG GCACCATACC AGCCACACGC ACCGTACCAA 
CCACCAAATG AACCGCCTGC ACCGTACCAA CCAGCTGCAC CGTACCATGC ACCACCAAAT 
GAACCTCCGG CACCATACCA GCCACACGCA CCCTACCAAC CACCAAATGA ACCTCCCGCA 
CCATACCAGC CACATGCACC GTACCAACCA CCAAATGAAC CTCCGGCACC ATACCAGCCA 
CATGCACCGT ACCAACCACC AAATGAACCT CCTGCACCGT ACCAACCGTA TCAACCTCCA 
AATGAACCGC CTGCACCATA CCAACCACCT GCACCGTACC AACCACCAAA TGAACCTCCG 
CCACCATACC AACCACATGC ACCGTACCAA CCTCCAAATG AACCTCCGGC ACCGTACCAA 
CCACCACATG GGCCTCCTGC ACCATACCAA CCACCAGCTG AAGCAACAGC AATAGCCGCA 
GAGTGCACCC AAGGTCTCCA AGCCGTGGTA GGGGAGAAGA AGTCCTTTGC TTCCCCTGGC 
TGGCCCGTCA GGTACCCCCT CCAGGTGCAG TGCTCGACCA CGATCACTGC CAGTGCCAAC 
GGCAGGATAA GGATAGAGTT CACTCACTTT GAATTGGAAG GAGACGAATG CAAATATGAC 
TATGTGCAGA TATCGGAACC CTACCAGAGT GGCACACCTG AGAAGCTGTG CGGCGTCAAA 
ACTCCGTTCG TCTATGAGAG CAAGTCTGGC CGCGTGGTTG TAACACTCGT CAGCGATGGT 
TACGTGACGA AAAAGGGCTT CCTCGCAACA TTTACGCAAT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030845102.1
CDS	99..6083
Translation	MWNRISGHALWLCTQHVLLVVLLLGCCHLPSVESDPTLTHRWAVTYGECSQTCGGGTQILVKSCYTLATNSQPSQTAPISYCSGLDHPPEPTVRSCSTQPCTMCDRTYTALSGALSSPYWPDKSSIPRYCRLVIDIEPDKRILLAFHEFTLEGIDLFSPSKTEEDVCLCGVLVEIRDDGGRMNRYCGTKKPFAWVSETDTVTLEFITPYSLVFNRFLASYTTIDTKPSCYRFIDLTGLHTGLEADGITEVPLPQKGFLHSLNFPNAYPDNQNCEYVLHGNPEHRIVLYFDEFELEPGPACEADYVKIEDFYSRRSNVYCGKHHSFSWISDSNEVWITFRSNANQVYTGFLLAYELMDREGNETIALEEESGFFVSPGYPLIYESDASAEYAIRTDPSKLLVVTFHDFQLERDDYPAPLPVGILEMDKRSTLNEHQNHYGDRYDLCVDYVKIIDIFDNHVETYCGRIPVFSYISRGNEILVQFKTDLNMNYRGFNASYRSISRRTPNDWELVYRVAKGGETEALDKFLMQDRGYSESDPNAKGTTITRSLATFKDSDVPNWENIGIRKVKVDAYKSGSIIATFRFDGHGTSLSDWFSCEKMTYSGYDDLNSKDCGGLVFGLTGMPPEFEITTTIANGKTCDDDMDHRVWLDVQDVAEEGDCTYEEPEIDINSIIFVSSATTGGETATGANIAEADVFTIWVQKDCDERISPARDSPVNIASKGHDELDYNNNEYCDLYLSVHPFDRLAIEFTDFELEEDECLANDYVQIENIETAFAQIFCGVQHQVVWTATANAAILRFRTDSVVTYNGWGASVRAERRNLEDCSTVTVNPTGNVTNPDYPNSLQDYRYYACEFIVHAHPEDRVIFSFSRFIFETENPPELQIDPRSTRANFIDVSGRLYPFSWTSDTNELALIYHAPLPYRTYIFFEGDYRTLEAPRPATCQRFADADLGGTFSWPTESLSTGLQNCYVIVRSDPVEHRRVQIIFDNLKMPQSDCNSYINTLDTTTRRNDMYCQRIRIPFAWTSDANEVVIKFFHGPTFALHQVNVLYRSRARVRPEDAKVLTALTGGDFSSPNYPQPYDSQSKKEYLLHAPAEHLVIVVFKDFSVEGAIEPSPLIEPVLRPGRSVEDLSAHYKHMYKRQTIVEPPTPECRYDYVSVEDVLVNTETKYCGDQQPFGVATQGNEALVLFLTDETVEKRGFTALYAFEPRPAISDCGTLQTADQGILQSPNYPNYYPDGAYCRDVIQVDPKNRIVIDFRFLNLEDDITCSKDYLEVRDVVTGRAERYCGHKATAFQWKSDTNLVFLTFVSDNANSDPGYDAGYTIEDRSDEPTCVHTITATSGTIHSPRFPRDYPNQLRCEYNIKVYPDYGIKLTFQEFNLEPPAPGSTDCAFDYLQIIDPTTVNVVTAEKLCGSKNTFERQIQFNEVSLIFVSDFITFYKGFLVDFQQISLGQTPQTVQTTSCNEQLTAPSGTFSSPGYPGNYPDNSLCVTLITVAVGYHVRINFNDFNTEGYTSCPYDYVLVQDVQVPGLDERFCGRKQHPFSWTSESNVVSVTFKSDDRTPSSGFYAAYESAPNVAAAVVAPVTGCETHYGITEISGTSGTVQSPDYPSLYPLNVDCFVRFTTGYGSRCQINFIVFNLEASSTCSYDYVKVTSIPDGATQTFCGSLAPFEWHSHSATCEIQFHSDSYQRASGYHAQITAVSSNIHAPYQSHQPPNEPASPYQPPNEPPAPYQPHAPYQPPNEPPAPYQPAAPYHAPPNEPPAPYQPHAPYQPPNEPPAPYQPHAPYQPPNEPPAPYQPHAPYQPPNEPPAPYQPYQPPNEPPAPYQPPAPYQPPNEPPPPYQPHAPYQPPNEPPAPYQPPHGPPAPYQPPAEATAIAAECTQGLQAVVGEKKSFASPGWPVRYPLQVQCSTTITASANGRIRIEFTHFELEGDECKYDYVQISEPYQSGTPEKLCGVKTPFVYESKSGRVVVTLVSDGYVTKKGFLATFTQF

Target ORF information:

RefSeq Version	XM_030989242.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus GTP-binding protein (mgb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030989242.1

ORF Insert Sequence:

ATGTGGAACC GTATCAGTGG ACACGCCCTT TGGTTATGCA CTCAGCACGT TTTATTAGTT 
GTCTTATTAC TGGGATGCTG CCATCTACCG TCGGTTGAAT CGGACCCAAC CCTGACGCAT 
AGATGGGCGG TTACATATGG AGAATGTAGT CAGACATGCG GTGGAGGTAC ACAAATCCTT 
GTCAAAAGCT GTTACACATT GGCCACGAAC TCACAGCCCT CACAGACGGC ACCCATTAGC 
TACTGCAGTG GACTGGACCA CCCACCAGAA CCAACCGTTC GATCATGCAG CACACAACCA 
TGCACAATGT GTGATCGTAC TTACACTGCC CTCAGTGGAG CGCTGTCATC ACCATATTGG 
CCTGATAAAT CATCAATACC CAGGTACTGT CGGCTTGTGA TAGATATTGA ACCTGATAAG 
AGGATCTTGT TGGCCTTCCA TGAGTTTACC CTCGAGGGTA TAGATCTGTT TAGTCCCTCC 
AAGACTGAAG AAGATGTCTG CTTGTGTGGT GTGCTCGTGG AGATACGGGA TGATGGTGGG 
CGCATGAATC GTTACTGTGG AACCAAGAAA CCGTTTGCTT GGGTCTCTGA GACGGATACA 
GTAACGCTGG AATTTATCAC ACCCTATTCT CTCGTCTTTA ATCGCTTCCT TGCATCTTAT 
ACCACCATCG ATACAAAACC AAGTTGCTAT CGCTTCATTG ACCTGACCGG CCTCCATACC 
GGTCTCGAGG CAGATGGAAT CACTGAGGTT CCTTTGCCTC AAAAAGGCTT CCTGCACTCG 
TTGAACTTCC CGAACGCCTA TCCCGACAAC CAGAACTGCG AGTACGTTCT CCATGGCAAT 
CCAGAGCATC GCATCGTGCT GTACTTTGAC GAGTTTGAGC TAGAACCTGG TCCGGCATGC 
GAGGCTGACT ATGTCAAGAT TGAGGATTTC TACAGTCGGC GCTCTAACGT CTACTGCGGC 
AAACATCACT CTTTCTCCTG GATCTCTGAT AGCAATGAAG TCTGGATCAC GTTCAGATCC 
AACGCCAATC AAGTCTACAC CGGTTTCCTT CTGGCCTACG AACTCATGGA TCGGGAAGGA 
AATGAGACGA TCGCGCTAGA AGAAGAGTCC GGTTTCTTTG TGTCGCCTGG TTACCCTCTG 
ATCTACGAGT CGGACGCGTC GGCGGAATAC GCCATCCGCA CTGACCCTAG CAAGCTCCTC 
GTCGTGACCT TCCACGACTT CCAGCTGGAG AGAGACGACT ACCCCGCTCC GCTGCCTGTG 
GGGATTCTGG AGATGGACAA GCGAAGCACG TTGAACGAGC ATCAGAATCA CTACGGCGAC 
CGATACGACT TATGTGTTGA TTACGTCAAG ATCATCGACA TTTTCGACAA TCATGTGGAA 
ACGTACTGTG GGCGGATCCC GGTGTTTAGC TATATCTCCC GTGGCAACGA GATCCTGGTC 
CAGTTTAAGA CCGATCTGAA CATGAACTAC AGAGGCTTCA ACGCGTCTTA CAGGTCCATC 
TCTAGAAGAA CACCAAATGA TTGGGAACTT GTCTACCGAG TGGCAAAAGG AGGCGAAACC 
GAGGCTCTTG ATAAGTTCCT GATGCAGGAT CGGGGCTACA GCGAGTCTGA CCCCAATGCA 
AAGGGAACCA CAATTACTCG CTCTCTTGCT ACCTTCAAGG ATTCAGATGT CCCAAATTGG 
GAGAATATCG GAATCAGAAA AGTCAAAGTT GACGCCTATA AGTCGGGAAG CATCATAGCA 
ACTTTCCGCT TTGATGGCCA TGGCACAAGT CTCAGTGACT GGTTTAGTTG CGAGAAGATG 
ACCTACAGCG GTTACGACGA CCTCAACTCA AAAGATTGTG GTGGTCTCGT GTTTGGCCTT 
ACAGGAATGC CGCCGGAGTT TGAAATCACA ACAACAATTG CCAACGGCAA GACGTGCGAT 
GATGACATGG ATCATAGGGT GTGGCTCGAT GTCCAAGATG TTGCGGAAGA GGGGGATTGT 
ACATACGAGG AGCCGGAAAT TGATATCAAC TCCATCATCT TCGTTAGCAG CGCTACGACG 
GGAGGGGAAA CTGCAACGGG AGCAAACATA GCCGAAGCAG ACGTGTTCAC CATCTGGGTT 
CAAAAAGATT GCGATGAAAG AATCTCGCCA GCCAGGGACA GCCCTGTAAA CATTGCTTCC 
AAGGGGCACG ATGAGCTTGA CTACAACAAT AACGAGTATT GCGATCTCTA TCTGAGTGTC 
CATCCGTTTG ATCGGCTCGC GATTGAATTC ACCGATTTTG AGCTGGAAGA AGATGAGTGT 
CTCGCAAATG ATTACGTCCA GATTGAGAAC ATAGAAACGG CCTTTGCGCA GATCTTCTGT 
GGTGTTCAAC ACCAAGTAGT ATGGACCGCT ACTGCGAATG CTGCCATCTT GAGGTTCAGA 
ACGGACAGTG TGGTAACGTA CAATGGTTGG GGTGCCAGTG TGAGAGCAGA ACGGCGAAAT 
TTGGAAGATT GTTCCACCGT CACCGTGAAC CCAACCGGCA ACGTGACCAA TCCCGATTAC 
CCCAACTCAC TCCAGGATTA TCGCTACTAC GCCTGCGAGT TCATCGTACA CGCCCATCCG 
GAGGACCGAG TTATCTTCTC ATTCTCGCGT TTCATCTTTG AGACAGAGAA CCCACCAGAG 
TTGCAGATTG ACCCAAGGTC AACCCGGGCC AACTTCATCG ATGTGTCGGG TCGTCTCTAC 
CCCTTCTCTT GGACCTCTGA TACCAACGAG CTAGCGCTTA TCTACCACGC CCCTCTTCCC 
TACCGGACCT ACATATTCTT TGAGGGCGAC TACCGCACCC TAGAGGCTCC TCGACCGGCC 
ACCTGCCAAC GGTTTGCGGA TGCCGATCTG GGAGGGACGT TCTCCTGGCC GACGGAATCT 
CTGTCGACCG GTCTGCAGAA CTGTTACGTG ATCGTGAGGT CCGACCCAGT CGAGCATAGA 
CGAGTCCAGA TAATCTTTGA CAATCTTAAG ATGCCCCAGT CGGATTGCAA TAGCTACATC 
AATACTCTTG ATACCACTAC AAGGCGTAAC GACATGTATT GTCAGAGAAT CCGCATCCCC 
TTCGCATGGA CCTCAGATGC CAATGAGGTC GTGATTAAAT TTTTCCATGG GCCGACCTTC 
GCTCTCCACC AGGTCAACGT ACTTTACAGG AGCCGAGCAA GGGTTCGTCC TGAAGATGCC 
AAAGTGTTGA CGGCTCTGAC GGGCGGTGAC TTCAGCAGCC CCAACTACCC CCAACCCTAC 
GATTCACAAT CCAAGAAGGA GTACCTCTTG CACGCCCCTG CAGAACATCT CGTCATTGTT 
GTCTTCAAAG ACTTCTCGGT TGAAGGTGCC ATAGAACCAA GTCCTCTTAT AGAGCCTGTA 
TTAAGGCCTG GCCGTAGCGT GGAGGACCTC TCCGCTCACT ACAAACACAT GTACAAGAGG 
CAGACGATAG TAGAGCCACC TACACCTGAA TGCCGCTACG ACTATGTTTC GGTTGAAGAC 
GTACTGGTGA ACACGGAGAC CAAGTACTGC GGGGATCAGC AACCGTTTGG TGTGGCGACT 
CAGGGCAACG AGGCCCTAGT CCTGTTCCTG ACTGATGAGA CCGTTGAGAA ACGAGGATTC 
ACCGCTCTCT ATGCTTTTGA GCCTCGACCG GCTATATCAG ACTGCGGTAC GCTGCAAACC 
GCGGACCAGG GCATCCTCCA GTCTCCGAAC TACCCTAACT ACTACCCGGA CGGGGCATAC 
TGCCGAGACG TGATACAGGT GGACCCAAAG AACCGCATCG TTATCGACTT TCGTTTCTTG 
AACTTGGAGG ATGATATCAC CTGCAGTAAA GACTACCTTG AGGTGCGTGA TGTAGTAACG 
GGGAGAGCGG AGAGGTACTG TGGACACAAG GCTACAGCCT TCCAATGGAA GTCGGACACC 
AACCTGGTCT TCCTGACGTT CGTCAGCGAC AATGCCAACA GCGATCCTGG ATACGATGCA 
GGATACACCA TAGAGGATAG ATCAGATGAA CCTACCTGCG TGCACACCAT CACGGCAACG 
TCCGGTACCA TCCACTCGCC GAGGTTCCCT CGAGACTACC CCAACCAGCT CAGGTGCGAG 
TACAACATCA AGGTGTATCC GGATTATGGA ATCAAGCTCA CCTTCCAAGA GTTCAACCTG 
GAGCCCCCCG CCCCGGGAAG CACGGACTGT GCCTTTGATT ATCTACAGAT TATTGACCCG 
ACGACGGTCA ACGTCGTGAC AGCCGAGAAG CTCTGCGGCT CCAAGAACAC TTTTGAACGT 
CAGATCCAGT TTAATGAAGT CAGTCTCATC TTTGTCAGTG ATTTCATCAC GTTCTACAAG 
GGTTTCCTGG TTGATTTCCA ACAGATCTCG CTCGGACAAA CCCCACAAAC CGTCCAAACT 
ACTAGTTGCA ATGAGCAGCT TACTGCTCCG TCAGGAACCT TCTCTAGCCC TGGTTACCCC 
GGTAACTATC CTGACAACTC CTTGTGCGTC ACCCTCATCA CTGTTGCCGT GGGTTACCAT 
GTCAGAATCA ACTTCAATGA CTTCAATACA GAAGGCTATA CAAGCTGCCC GTATGATTAT 
GTTCTGGTAC AAGATGTTCA GGTTCCTGGT CTGGACGAGA GGTTCTGCGG GCGCAAGCAG 
CACCCTTTCT CGTGGACCTC GGAAAGTAAC GTGGTTTCAG TGACGTTCAA GAGTGACGAT 
AGGACACCCT CGTCCGGTTT CTATGCAGCG TACGAATCAG CGCCGAACGT AGCCGCCGCC 
GTCGTTGCCC CAGTCACAGG TTGTGAGACG CACTACGGCA TCACCGAAAT CAGCGGAACT 
TCAGGAACCG TGCAGTCTCC GGACTATCCT AGCCTCTATC CACTCAATGT TGACTGCTTC 
GTCCGCTTCA CGACCGGTTA CGGATCTCGA TGCCAAATCA ACTTCATCGT CTTCAACCTG 
GAGGCCTCAA GCACATGCAG TTACGACTAT GTAAAGGTAA CGAGTATTCC AGATGGCGCA 
ACCCAGACGT TCTGCGGGTC TCTTGCTCCA TTTGAATGGC ACTCCCATTC TGCTACATGC 
GAGATCCAGT TCCACAGCGA CTCCTACCAA AGAGCATCGG GCTACCATGC ACAAATCACA 
GCTGTTTCTT CAAATATCCA TGCACCCTAC CAATCGCATC AACCTCCAAA TGAACCAGCT 
TCACCGTACC AACCACCAAA TGAACCTCCG GCACCATACC AGCCACACGC ACCGTACCAA 
CCACCAAATG AACCGCCTGC ACCGTACCAA CCAGCTGCAC CGTACCATGC ACCACCAAAT 
GAACCTCCGG CACCATACCA GCCACACGCA CCCTACCAAC CACCAAATGA ACCTCCCGCA 
CCATACCAGC CACATGCACC GTACCAACCA CCAAATGAAC CTCCGGCACC ATACCAGCCA 
CATGCACCGT ACCAACCACC AAATGAACCT CCTGCACCGT ACCAACCGTA TCAACCTCCA 
AATGAACCGC CTGCACCATA CCAACCACCT GCACCGTACC AACCACCAAA TGAACCTCCG 
CCACCATACC AACCACATGC ACCGTACCAA CCTCCAAATG AACCTCCGGC ACCGTACCAA 
CCACCACATG GGCCTCCTGC ACCATACCAA CCACCAGCTG AAGCAACAGC AATAGCCGCA 
GAGTGCACCC AAGGTCTCCA AGCCGTGGTA GGGGAGAAGA AGTCCTTTGC TTCCCCTGGC 
TGGCCCGTCA GGTACCCCCT CCAGGTGCAG TGCTCGACCA CGATCACTGC CAGTGCCAAC 
GGCAGGATAA GGATAGAGTT CACTCACTTT GAATTGGAAG GAGACGAATG CAAATATGAC 
TATGTGCAGA TATCGGAACC CTACCAGAGT GGCACACCTG AGAAGCTGTG CGGCGTCAAA 
ACTCCGTTCG TCTATGAGAG CAAGTCTGGC CGCGTGGTTG TAACACTCGT CAGCGATGGT 
TACGTGACGA AAAAGGGCTT CCTCGCAACA TTTACGCAAT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mgb ( XM_030989243.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030845103.1 Strongylocentrotus purpuratus GTP-binding protein (mgb), transcript variant X3, mRNA.

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CloneID	OSd55359
Clone ID Related Accession (Same CDS sequence)	XM_030989243.1
Accession Version	XM_030989243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	GTP-binding protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145603.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACC GTATCAGTGG ACACGCCCTT TGGTTATGCA CTCAGCACGT TTTATTAGTT 
GTCTTATTAC TGGGATGCTG CCATCTACCG TCGGTTGAAT CGGACCCAAC CCTGACGCAT 
AGATGGGCGG TTACATATGG AGAATGTAGT CAGACATGCG GTGGAGGTAC ACAAATCCTT 
GTCAAAAGCT GTTACACATT GGCCACGAAC TCACAGCCCT CACAGACGGC ACCCATTAGC 
TACTGCAGTG GACTGGACCA CCCACCAGAA CCAACCGTTC GATCATGCAG CACACAACCA 
TGCACAATGT GTGATCGTAC TTACACTGCC CTCAGTGGAG CGCTGTCATC ACCATATTGG 
CCTGATAAAT CATCAATACC CAGGTACTGT CGGCTTGTGA TAGATATTGA ACCTGATAAG 
AGGATCTTGT TGGCCTTCCA TGAGTTTACC CTCGAGGGTA TAGATCTGTT TAGTCCCTCC 
AAGACTGAAG AAGATGTCTG CTTGTGTGGT GTGCTCGTGG AGATACGGGA TGATGGTGGG 
CGCATGAATC GTTACTGTGG AACCAAGAAA CCGTTTGCTT GGGTCTCTGA GACGGATACA 
GTAACGCTGG AATTTATCAC ACCCTATTCT CTCGTCTTTA ATCGCTTCCT TGCATCTTAT 
ACCACCATCG ATACAAAACC AAGTTGCTAT CGCTTCATTG ACCTGACCGG CCTCCATACC 
GGTCTCGAGG CAGATGGAAT CACTGAGGTT CCTTTGCCTC AAAAAGGCTT CCTGCACTCG 
TTGAACTTCC CGAACGCCTA TCCCGACAAC CAGAACTGCG AGTACGTTCT CCATGGCAAT 
CCAGAGCATC GCATCGTGCT GTACTTTGAC GAGTTTGAGC TAGAACCTGG TCCGGCATGC 
GAGGCTGACT ATGTCAAGAT TGAGGATTTC TACAGTCGGC GCTCTAACGT CTACTGCGGC 
AAACATCACT CTTTCTCCTG GATCTCTGAT AGCAATGAAG TCTGGATCAC GTTCAGATCC 
AACGCCAATC AAGTCTACAC CGGTTTCCTT CTGGCCTACG AACTCATGGA TCGGGAAGGA 
AATGAGACGA TCGCGCTAGA AGAAGAGTCC GGTTTCTTTG TGTCGCCTGG TTACCCTCTG 
ATCTACGAGT CGGACGCGTC GGCGGAATAC GCCATCCGCA CTGACCCTAG CAAGCTCCTC 
GTCGTGACCT TCCACGACTT CCAGCTGGAG AGAGACGACT ACCCCGCTCC GCTGCCTGTG 
GGGATTCTGG AGATGGACAA GCGAAGCACG TTGAACGAGC ATCAGAATCA CTACGGCGAC 
CGATACGACT TATGTGTTGA TTACGTCAAG ATCATCGACA TTTTCGACAA TCATGTGGAA 
ACGTACTGTG GGCGGATCCC GGTGTTTAGC TATATCTCCC GTGGCAACGA GATCCTGGTC 
CAGTTTAAGA CCGATCTGAA CATGAACTAC AGAGGCTTCA ACGCGTCTTA CAGGTCCATC 
TCTAGAAGAA CACCAAATGA TTGGGAACTT GTCTACCGAG TGGCAAAAGG AGGCGAAACC 
GAGGCTCTTG ATAAGTTCCT GATGCAGGAT CGGGGCTACA GCGAGTCTGA CCCCAATGCA 
AAGGGAACCA CAATTACTCG CTCTCTTGCT ACCTTCAAGG ATTCAGATGT CCCAAATTGG 
GAGAATATCG GAATCAGAAA AGTCAAAGTT GACGCCTATA AGTCGGGAAG CATCATAGCA 
ACTTTCCGCT TTGATGGCCA TGGCACAAGT CTCAGTGACT GGTTTAGTTG CGAGAAGATG 
ACCTACAGCG GTTACGACGA CCTCAACTCA AAAGATTGTG GTGGTCTCGT GTTTGGCCTT 
ACAGGAATGC CGCCGGAGTT TGAAATCACA ACAACAATTG CCAACGGCAA GACGTGCGAT 
GATGACATGG ATCATAGGGT GTGGCTCGAT GTCCAAGATG TTGCGGAAGA GGGGGATTGT 
ACATACGAGG AGCCGGAAAT TGATATCAAC TCCATCATCT TCGTTAGCAG CGCTACGACG 
GGAGGGGAAA CTGCAACGGG AGCAAACATA GCCGAAGCAG ACGTGTTCAC CATCTGGGTT 
CAAAAAGATT GCGATGAAAG AATCTCGCCA GCCAGGGACA GCCCTGTAAA CATTGCTTCC 
AAGGGGCACG ATGAGCTTGA CTACAACAAT AACGAGTATT GCGATCTCTA TCTGAGTGTC 
CATCCGTTTG ATCGGCTCGC GATTGAATTC ACCGATTTTG AGCTGGAAGA AGATGAGTGT 
CTCGCAAATG ATTACGTCCA GATTGAGAAC ATAGAAACGG CCTTTGCGCA GATCTTCTGT 
GGTGTTCAAC ACCAAGTAGT ATGGACCGCT ACTGCGAATG CTGCCATCTT GAGGTTCAGA 
ACGGACAGTG TGGTAACGTA CAATGGTTGG GGTGCCAGTG TGAGAGCAGA ACGGCGAAAT 
TTGGAAGATT GTTCCACCGT CACCGTGAAC CCAACCGGCA ACGTGACCAA TCCCGATTAC 
CCCAACTCAC TCCAGGATTA TCGCTACTAC GCCTGCGAGT TCATCGTACA CGCCCATCCG 
GAGGACCGAG TTATCTTCTC ATTCTCGCGT TTCATCTTTG AGACAGAGAA CCCACCAGAG 
TTGCAGATTG ACCCAAGGTC AACCCGGGCC AACTTCATCG ATGTGTCGGG TCGTCTCTAC 
CCCTTCTCTT GGACCTCTGA TACCAACGAG CTAGCGCTTA TCTACCACGC CCCTCTTCCC 
TACCGGACCT ACATATTCTT TGAGGGCGAC TACCGCACCC TAGAGGCTCC TCGACCGGCC 
ACCTGCCAAC GGTTTGCGGA TGCCGATCTG GGAGGGACGT TCTCCTGGCC GACGGAATCT 
CTGTCGACCG GTCTGCAGAA CTGTTACGTG ATCGTGAGGT CCGACCCAGT CGAGCATAGA 
CGAGTCCAGA TAATCTTTGA CAATCTTAAG ATGCCCCAGT CGGATTGCAA TAGCTACATC 
AATACTCTTG ATACCACTAC AAGGCGTAAC GACATGTATT GTCAGAGAAT CCGCATCCCC 
TTCGCATGGA CCTCAGATGC CAATGAGGTC GTGATTAAAT TTTTCCATGG GCCGACCTTC 
GCTCTCCACC AGGTCAACGT ACTTTACAGG AGCCGAGCAA GGGTTCGTCC TGAAGATGCC 
AAAGTGTTGA CGGCTCTGAC GGGCGGTGAC TTCAGCAGCC CCAACTACCC CCAACCCTAC 
GATTCACAAT CCAAGAAGGA GTACCTCTTG CACGCCCCTG CAGAACATCT CGTCATTGTT 
GTCTTCAAAG ACTTCTCGGT TGAAGGTGCC ATAGAACCAA GTCCTCTTAT AGAGCCTGTA 
TTAAGGCCTG GCCGTAGCGT GGAGGACCTC TCCGCTCACT ACAAACACAT GTACAAGAGG 
CAGACGATAG TAGAGCCACC TACACCTGAA TGCCGCTACG ACTATGTTTC GGTTGAAGAC 
GTACTGGTGA ACACGGAGAC CAAGTACTGC GGGGATCAGC AACCGTTTGG TGTGGCGACT 
CAGGGCAACG AGGCCCTAGT CCTGTTCCTG ACTGATGAGA CCGTTGAGAA ACGAGGATTC 
ACCGCTCTCT ATGCTTTTGA GCCTCGACCG GCTATATCAG ACTGCGGTAC GCTGCAAACC 
GCGGACCAGG GCATCCTCCA GTCTCCGAAC TACCCTAACT ACTACCCGGA CGGGGCATAC 
TGCCGAGACG TGATACAGGT GGACCCAAAG AACCGCATCG TTATCGACTT TCGTTTCTTG 
AACTTGGAGG ATGATATCAC CTGCAGTAAA GACTACCTTG AGGTGCGTGA TGTAGTAACG 
GGGAGAGCGG AGAGGTACTG TGGACACAAG GCTACAGCCT TCCAATGGAA GTCGGACACC 
AACCTGGTCT TCCTGACGTT CGTCAGCGAC AATGCCAACA GCGATCCTGG ATACGATGCA 
GGATACACCA TAGAGGATAG ATCAGATGAA CCTACCTGCG TGCACACCAT CACGGCAACG 
TCCGGTACCA TCCACTCGCC GAGGTTCCCT CGAGACTACC CCAACCAGCT CAGGTGCGAG 
TACAACATCA AGGTGTATCC GGATTATGGA ATCAAGCTCA CCTTCCAAGA GTTCAACCTG 
GAGCCCCCCG CCCCGGGAAG CACGGACTGT GCCTTTGATT ATCTACAGAT TATTGACCCG 
ACGACGGTCA ACGTCGTGAC AGCCGAGAAG CTCTGCGGCT CCAAGAACAC TTTTGAACGT 
CAGATCCAGT TTAATGAAGT CAGTCTCATC TTTGTCAGTG ATTTCATCAC GTTCTACAAG 
GGTTTCCTGG TTGATTTCCA ACAGATCTCG CTCGGACAAA CCCCACAAAC CGTCCAAACT 
ACTAGTTGCA ATGAGCAGCT TACTGCTCCG TCAGGAACCT TCTCTAGCCC TGGTTACCCC 
GGTAACTATC CTGACAACTC CTTGTGCGTC ACCCTCATCA CTGTTGCCGT GGGTTACCAT 
GTCAGAATCA ACTTCAATGA CTTCAATACA GAAGGCTATA CAAGCTGCCC GTATGATTAT 
GTTCTGGTAC AAGATGTTCA GGTTCCTGGT CTGGACGAGA GGTTCTGCGG GCGCAAGCAG 
CACCCTTTCT CGTGGACCTC GGAAAGTAAC GTGGTTTCAG TGACGTTCAA GAGTGACGAT 
AGGACACCCT CGTCCGGTTT CTATGCAGCG TACGAATCAG CGCCGAACGT AGCCGCCGCC 
GTCGTTGCCC CAGTCACAGG TTGTGAGACG CACTACGGCA TCACCGAAAT CAGCGGAACT 
TCAGGAACCG TGCAGTCTCC GGACTATCCT AGCCTCTATC CACTCAATGT TGACTGCTTC 
GTCCGCTTCA CGACCGGTTA CGGATCTCGA TGCCAAATCA ACTTCATCGT CTTCAACCTG 
GAGGCCTCAA GCACATGCAG TTACGACTAT GTAAAGGTAA CGAGTATTCC AGATGGCGCA 
ACCCAGACGT TCTGCGGGTC TCTTGCTCCA TTTGAATGGC ACTCCCATTC TGCTACATGC 
GAGATCCAGT TCCACAGCGA CTCCTACCAA AGAGCATCGG GCTACCATGC ACAAATCACA 
GCTGTTTCTT CAAATATCCA TGCACCCTAC CAATCGCATC AACCTCCAAA TGAACCAGCT 
TCACCGTACC AACCATACCA ACCACCAAAT GAACCTCCGG CACCATACCA GCCACATGCA 
CCGTACCAAC CACCAAATGA ACCTCCTGCA CCGTACCAAC CGTATCAACC TCCAAATGAA 
CCGCCTGCAC CATACCAACC ACCTGCACCG TACCAACCAC CAAATGAACC TCCGCCACCA 
TACCAACCAC ATGCACCGTA CCAACCTCCA AATGAACCTC CGGCACCGTA CCAACCACAT 
GCACCGTACC CACCACCAAA TGAACCACCA GCACCATACC AACCACCACA TGGGCCTCCT 
GCACCATACC AACCACCAGC TGAAGCAACA GCAATAGCCG CAGAGTGCAC CCAAGGTCTC 
CAAGCCGTGG TAGGGGAGAA GAAGTCCTTT GCTTCCCCTG GCTGGCCCGT CAGGTACCCC 
CTCCAGGTGC AGTGCTCGAC CACGATCACT GCCAGTGCCA ACGGCAGGAT AAGGATAGAG 
TTCACTCACT TTGAATTGGA AGGAGACGAA TGCAAATATG ACTATGTGCA GATATCGGAA 
CCCTACCAGA GTGGCACACC TGAGAAGCTG TGCGGCGTCA AAACTCCGTT CGTCTATGAG 
AGCAAGTCTG GCCGCGTGGT TGTAACACTC GTCAGCGATG GTTACGTGAC GAAAAAGGGC 
TTCCTCGCAA CATTTACGCA ATTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030845103.1
CDS	99..5945
Translation	MWNRISGHALWLCTQHVLLVVLLLGCCHLPSVESDPTLTHRWAVTYGECSQTCGGGTQILVKSCYTLATNSQPSQTAPISYCSGLDHPPEPTVRSCSTQPCTMCDRTYTALSGALSSPYWPDKSSIPRYCRLVIDIEPDKRILLAFHEFTLEGIDLFSPSKTEEDVCLCGVLVEIRDDGGRMNRYCGTKKPFAWVSETDTVTLEFITPYSLVFNRFLASYTTIDTKPSCYRFIDLTGLHTGLEADGITEVPLPQKGFLHSLNFPNAYPDNQNCEYVLHGNPEHRIVLYFDEFELEPGPACEADYVKIEDFYSRRSNVYCGKHHSFSWISDSNEVWITFRSNANQVYTGFLLAYELMDREGNETIALEEESGFFVSPGYPLIYESDASAEYAIRTDPSKLLVVTFHDFQLERDDYPAPLPVGILEMDKRSTLNEHQNHYGDRYDLCVDYVKIIDIFDNHVETYCGRIPVFSYISRGNEILVQFKTDLNMNYRGFNASYRSISRRTPNDWELVYRVAKGGETEALDKFLMQDRGYSESDPNAKGTTITRSLATFKDSDVPNWENIGIRKVKVDAYKSGSIIATFRFDGHGTSLSDWFSCEKMTYSGYDDLNSKDCGGLVFGLTGMPPEFEITTTIANGKTCDDDMDHRVWLDVQDVAEEGDCTYEEPEIDINSIIFVSSATTGGETATGANIAEADVFTIWVQKDCDERISPARDSPVNIASKGHDELDYNNNEYCDLYLSVHPFDRLAIEFTDFELEEDECLANDYVQIENIETAFAQIFCGVQHQVVWTATANAAILRFRTDSVVTYNGWGASVRAERRNLEDCSTVTVNPTGNVTNPDYPNSLQDYRYYACEFIVHAHPEDRVIFSFSRFIFETENPPELQIDPRSTRANFIDVSGRLYPFSWTSDTNELALIYHAPLPYRTYIFFEGDYRTLEAPRPATCQRFADADLGGTFSWPTESLSTGLQNCYVIVRSDPVEHRRVQIIFDNLKMPQSDCNSYINTLDTTTRRNDMYCQRIRIPFAWTSDANEVVIKFFHGPTFALHQVNVLYRSRARVRPEDAKVLTALTGGDFSSPNYPQPYDSQSKKEYLLHAPAEHLVIVVFKDFSVEGAIEPSPLIEPVLRPGRSVEDLSAHYKHMYKRQTIVEPPTPECRYDYVSVEDVLVNTETKYCGDQQPFGVATQGNEALVLFLTDETVEKRGFTALYAFEPRPAISDCGTLQTADQGILQSPNYPNYYPDGAYCRDVIQVDPKNRIVIDFRFLNLEDDITCSKDYLEVRDVVTGRAERYCGHKATAFQWKSDTNLVFLTFVSDNANSDPGYDAGYTIEDRSDEPTCVHTITATSGTIHSPRFPRDYPNQLRCEYNIKVYPDYGIKLTFQEFNLEPPAPGSTDCAFDYLQIIDPTTVNVVTAEKLCGSKNTFERQIQFNEVSLIFVSDFITFYKGFLVDFQQISLGQTPQTVQTTSCNEQLTAPSGTFSSPGYPGNYPDNSLCVTLITVAVGYHVRINFNDFNTEGYTSCPYDYVLVQDVQVPGLDERFCGRKQHPFSWTSESNVVSVTFKSDDRTPSSGFYAAYESAPNVAAAVVAPVTGCETHYGITEISGTSGTVQSPDYPSLYPLNVDCFVRFTTGYGSRCQINFIVFNLEASSTCSYDYVKVTSIPDGATQTFCGSLAPFEWHSHSATCEIQFHSDSYQRASGYHAQITAVSSNIHAPYQSHQPPNEPASPYQPYQPPNEPPAPYQPHAPYQPPNEPPAPYQPYQPPNEPPAPYQPPAPYQPPNEPPPPYQPHAPYQPPNEPPAPYQPHAPYPPPNEPPAPYQPPHGPPAPYQPPAEATAIAAECTQGLQAVVGEKKSFASPGWPVRYPLQVQCSTTITASANGRIRIEFTHFELEGDECKYDYVQISEPYQSGTPEKLCGVKTPFVYESKSGRVVVTLVSDGYVTKKGFLATFTQF

Target ORF information:

RefSeq Version	XM_030989243.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus GTP-binding protein (mgb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030989243.1

ORF Insert Sequence:

ATGTGGAACC GTATCAGTGG ACACGCCCTT TGGTTATGCA CTCAGCACGT TTTATTAGTT 
GTCTTATTAC TGGGATGCTG CCATCTACCG TCGGTTGAAT CGGACCCAAC CCTGACGCAT 
AGATGGGCGG TTACATATGG AGAATGTAGT CAGACATGCG GTGGAGGTAC ACAAATCCTT 
GTCAAAAGCT GTTACACATT GGCCACGAAC TCACAGCCCT CACAGACGGC ACCCATTAGC 
TACTGCAGTG GACTGGACCA CCCACCAGAA CCAACCGTTC GATCATGCAG CACACAACCA 
TGCACAATGT GTGATCGTAC TTACACTGCC CTCAGTGGAG CGCTGTCATC ACCATATTGG 
CCTGATAAAT CATCAATACC CAGGTACTGT CGGCTTGTGA TAGATATTGA ACCTGATAAG 
AGGATCTTGT TGGCCTTCCA TGAGTTTACC CTCGAGGGTA TAGATCTGTT TAGTCCCTCC 
AAGACTGAAG AAGATGTCTG CTTGTGTGGT GTGCTCGTGG AGATACGGGA TGATGGTGGG 
CGCATGAATC GTTACTGTGG AACCAAGAAA CCGTTTGCTT GGGTCTCTGA GACGGATACA 
GTAACGCTGG AATTTATCAC ACCCTATTCT CTCGTCTTTA ATCGCTTCCT TGCATCTTAT 
ACCACCATCG ATACAAAACC AAGTTGCTAT CGCTTCATTG ACCTGACCGG CCTCCATACC 
GGTCTCGAGG CAGATGGAAT CACTGAGGTT CCTTTGCCTC AAAAAGGCTT CCTGCACTCG 
TTGAACTTCC CGAACGCCTA TCCCGACAAC CAGAACTGCG AGTACGTTCT CCATGGCAAT 
CCAGAGCATC GCATCGTGCT GTACTTTGAC GAGTTTGAGC TAGAACCTGG TCCGGCATGC 
GAGGCTGACT ATGTCAAGAT TGAGGATTTC TACAGTCGGC GCTCTAACGT CTACTGCGGC 
AAACATCACT CTTTCTCCTG GATCTCTGAT AGCAATGAAG TCTGGATCAC GTTCAGATCC 
AACGCCAATC AAGTCTACAC CGGTTTCCTT CTGGCCTACG AACTCATGGA TCGGGAAGGA 
AATGAGACGA TCGCGCTAGA AGAAGAGTCC GGTTTCTTTG TGTCGCCTGG TTACCCTCTG 
ATCTACGAGT CGGACGCGTC GGCGGAATAC GCCATCCGCA CTGACCCTAG CAAGCTCCTC 
GTCGTGACCT TCCACGACTT CCAGCTGGAG AGAGACGACT ACCCCGCTCC GCTGCCTGTG 
GGGATTCTGG AGATGGACAA GCGAAGCACG TTGAACGAGC ATCAGAATCA CTACGGCGAC 
CGATACGACT TATGTGTTGA TTACGTCAAG ATCATCGACA TTTTCGACAA TCATGTGGAA 
ACGTACTGTG GGCGGATCCC GGTGTTTAGC TATATCTCCC GTGGCAACGA GATCCTGGTC 
CAGTTTAAGA CCGATCTGAA CATGAACTAC AGAGGCTTCA ACGCGTCTTA CAGGTCCATC 
TCTAGAAGAA CACCAAATGA TTGGGAACTT GTCTACCGAG TGGCAAAAGG AGGCGAAACC 
GAGGCTCTTG ATAAGTTCCT GATGCAGGAT CGGGGCTACA GCGAGTCTGA CCCCAATGCA 
AAGGGAACCA CAATTACTCG CTCTCTTGCT ACCTTCAAGG ATTCAGATGT CCCAAATTGG 
GAGAATATCG GAATCAGAAA AGTCAAAGTT GACGCCTATA AGTCGGGAAG CATCATAGCA 
ACTTTCCGCT TTGATGGCCA TGGCACAAGT CTCAGTGACT GGTTTAGTTG CGAGAAGATG 
ACCTACAGCG GTTACGACGA CCTCAACTCA AAAGATTGTG GTGGTCTCGT GTTTGGCCTT 
ACAGGAATGC CGCCGGAGTT TGAAATCACA ACAACAATTG CCAACGGCAA GACGTGCGAT 
GATGACATGG ATCATAGGGT GTGGCTCGAT GTCCAAGATG TTGCGGAAGA GGGGGATTGT 
ACATACGAGG AGCCGGAAAT TGATATCAAC TCCATCATCT TCGTTAGCAG CGCTACGACG 
GGAGGGGAAA CTGCAACGGG AGCAAACATA GCCGAAGCAG ACGTGTTCAC CATCTGGGTT 
CAAAAAGATT GCGATGAAAG AATCTCGCCA GCCAGGGACA GCCCTGTAAA CATTGCTTCC 
AAGGGGCACG ATGAGCTTGA CTACAACAAT AACGAGTATT GCGATCTCTA TCTGAGTGTC 
CATCCGTTTG ATCGGCTCGC GATTGAATTC ACCGATTTTG AGCTGGAAGA AGATGAGTGT 
CTCGCAAATG ATTACGTCCA GATTGAGAAC ATAGAAACGG CCTTTGCGCA GATCTTCTGT 
GGTGTTCAAC ACCAAGTAGT ATGGACCGCT ACTGCGAATG CTGCCATCTT GAGGTTCAGA 
ACGGACAGTG TGGTAACGTA CAATGGTTGG GGTGCCAGTG TGAGAGCAGA ACGGCGAAAT 
TTGGAAGATT GTTCCACCGT CACCGTGAAC CCAACCGGCA ACGTGACCAA TCCCGATTAC 
CCCAACTCAC TCCAGGATTA TCGCTACTAC GCCTGCGAGT TCATCGTACA CGCCCATCCG 
GAGGACCGAG TTATCTTCTC ATTCTCGCGT TTCATCTTTG AGACAGAGAA CCCACCAGAG 
TTGCAGATTG ACCCAAGGTC AACCCGGGCC AACTTCATCG ATGTGTCGGG TCGTCTCTAC 
CCCTTCTCTT GGACCTCTGA TACCAACGAG CTAGCGCTTA TCTACCACGC CCCTCTTCCC 
TACCGGACCT ACATATTCTT TGAGGGCGAC TACCGCACCC TAGAGGCTCC TCGACCGGCC 
ACCTGCCAAC GGTTTGCGGA TGCCGATCTG GGAGGGACGT TCTCCTGGCC GACGGAATCT 
CTGTCGACCG GTCTGCAGAA CTGTTACGTG ATCGTGAGGT CCGACCCAGT CGAGCATAGA 
CGAGTCCAGA TAATCTTTGA CAATCTTAAG ATGCCCCAGT CGGATTGCAA TAGCTACATC 
AATACTCTTG ATACCACTAC AAGGCGTAAC GACATGTATT GTCAGAGAAT CCGCATCCCC 
TTCGCATGGA CCTCAGATGC CAATGAGGTC GTGATTAAAT TTTTCCATGG GCCGACCTTC 
GCTCTCCACC AGGTCAACGT ACTTTACAGG AGCCGAGCAA GGGTTCGTCC TGAAGATGCC 
AAAGTGTTGA CGGCTCTGAC GGGCGGTGAC TTCAGCAGCC CCAACTACCC CCAACCCTAC 
GATTCACAAT CCAAGAAGGA GTACCTCTTG CACGCCCCTG CAGAACATCT CGTCATTGTT 
GTCTTCAAAG ACTTCTCGGT TGAAGGTGCC ATAGAACCAA GTCCTCTTAT AGAGCCTGTA 
TTAAGGCCTG GCCGTAGCGT GGAGGACCTC TCCGCTCACT ACAAACACAT GTACAAGAGG 
CAGACGATAG TAGAGCCACC TACACCTGAA TGCCGCTACG ACTATGTTTC GGTTGAAGAC 
GTACTGGTGA ACACGGAGAC CAAGTACTGC GGGGATCAGC AACCGTTTGG TGTGGCGACT 
CAGGGCAACG AGGCCCTAGT CCTGTTCCTG ACTGATGAGA CCGTTGAGAA ACGAGGATTC 
ACCGCTCTCT ATGCTTTTGA GCCTCGACCG GCTATATCAG ACTGCGGTAC GCTGCAAACC 
GCGGACCAGG GCATCCTCCA GTCTCCGAAC TACCCTAACT ACTACCCGGA CGGGGCATAC 
TGCCGAGACG TGATACAGGT GGACCCAAAG AACCGCATCG TTATCGACTT TCGTTTCTTG 
AACTTGGAGG ATGATATCAC CTGCAGTAAA GACTACCTTG AGGTGCGTGA TGTAGTAACG 
GGGAGAGCGG AGAGGTACTG TGGACACAAG GCTACAGCCT TCCAATGGAA GTCGGACACC 
AACCTGGTCT TCCTGACGTT CGTCAGCGAC AATGCCAACA GCGATCCTGG ATACGATGCA 
GGATACACCA TAGAGGATAG ATCAGATGAA CCTACCTGCG TGCACACCAT CACGGCAACG 
TCCGGTACCA TCCACTCGCC GAGGTTCCCT CGAGACTACC CCAACCAGCT CAGGTGCGAG 
TACAACATCA AGGTGTATCC GGATTATGGA ATCAAGCTCA CCTTCCAAGA GTTCAACCTG 
GAGCCCCCCG CCCCGGGAAG CACGGACTGT GCCTTTGATT ATCTACAGAT TATTGACCCG 
ACGACGGTCA ACGTCGTGAC AGCCGAGAAG CTCTGCGGCT CCAAGAACAC TTTTGAACGT 
CAGATCCAGT TTAATGAAGT CAGTCTCATC TTTGTCAGTG ATTTCATCAC GTTCTACAAG 
GGTTTCCTGG TTGATTTCCA ACAGATCTCG CTCGGACAAA CCCCACAAAC CGTCCAAACT 
ACTAGTTGCA ATGAGCAGCT TACTGCTCCG TCAGGAACCT TCTCTAGCCC TGGTTACCCC 
GGTAACTATC CTGACAACTC CTTGTGCGTC ACCCTCATCA CTGTTGCCGT GGGTTACCAT 
GTCAGAATCA ACTTCAATGA CTTCAATACA GAAGGCTATA CAAGCTGCCC GTATGATTAT 
GTTCTGGTAC AAGATGTTCA GGTTCCTGGT CTGGACGAGA GGTTCTGCGG GCGCAAGCAG 
CACCCTTTCT CGTGGACCTC GGAAAGTAAC GTGGTTTCAG TGACGTTCAA GAGTGACGAT 
AGGACACCCT CGTCCGGTTT CTATGCAGCG TACGAATCAG CGCCGAACGT AGCCGCCGCC 
GTCGTTGCCC CAGTCACAGG TTGTGAGACG CACTACGGCA TCACCGAAAT CAGCGGAACT 
TCAGGAACCG TGCAGTCTCC GGACTATCCT AGCCTCTATC CACTCAATGT TGACTGCTTC 
GTCCGCTTCA CGACCGGTTA CGGATCTCGA TGCCAAATCA ACTTCATCGT CTTCAACCTG 
GAGGCCTCAA GCACATGCAG TTACGACTAT GTAAAGGTAA CGAGTATTCC AGATGGCGCA 
ACCCAGACGT TCTGCGGGTC TCTTGCTCCA TTTGAATGGC ACTCCCATTC TGCTACATGC 
GAGATCCAGT TCCACAGCGA CTCCTACCAA AGAGCATCGG GCTACCATGC ACAAATCACA 
GCTGTTTCTT CAAATATCCA TGCACCCTAC CAATCGCATC AACCTCCAAA TGAACCAGCT 
TCACCGTACC AACCATACCA ACCACCAAAT GAACCTCCGG CACCATACCA GCCACATGCA 
CCGTACCAAC CACCAAATGA ACCTCCTGCA CCGTACCAAC CGTATCAACC TCCAAATGAA 
CCGCCTGCAC CATACCAACC ACCTGCACCG TACCAACCAC CAAATGAACC TCCGCCACCA 
TACCAACCAC ATGCACCGTA CCAACCTCCA AATGAACCTC CGGCACCGTA CCAACCACAT 
GCACCGTACC CACCACCAAA TGAACCACCA GCACCATACC AACCACCACA TGGGCCTCCT 
GCACCATACC AACCACCAGC TGAAGCAACA GCAATAGCCG CAGAGTGCAC CCAAGGTCTC 
CAAGCCGTGG TAGGGGAGAA GAAGTCCTTT GCTTCCCCTG GCTGGCCCGT CAGGTACCCC 
CTCCAGGTGC AGTGCTCGAC CACGATCACT GCCAGTGCCA ACGGCAGGAT AAGGATAGAG 
TTCACTCACT TTGAATTGGA AGGAGACGAA TGCAAATATG ACTATGTGCA GATATCGGAA 
CCCTACCAGA GTGGCACACC TGAGAAGCTG TGCGGCGTCA AAACTCCGTT CGTCTATGAG 
AGCAAGTCTG GCCGCGTGGT TGTAACACTC GTCAGCGATG GTTACGTGAC GAAAAAGGGC 
TTCCTCGCAA CATTTACGCA ATTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mgb ( XM_030989241.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030845101.1 Strongylocentrotus purpuratus GTP-binding protein (mgb), transcript variant X1, mRNA.

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CloneID	OSd55357
Clone ID Related Accession (Same CDS sequence)	XM_030989241.1
Accession Version	XM_030989241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	GTP-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145603.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACC GTATCAGTGG ACACGCCCTT TGGTTATGCA CTCAGCACGT TTTATTAGTT 
GTCTTATTAC TGGGATGCTG CCATCTACCG TCGGTTGAAT CGGACCCAAC CCTGACGCAT 
AGATGGGCGG TTACATATGG AGAATGTAGT CAGACATGCG GTGGAGGTAC ACAAATCCTT 
GTCAAAAGCT GTTACACATT GGCCACGAAC TCACAGCCCT CACAGACGGC ACCCATTAGC 
TACTGCAGTG GACTGGACCA CCCACCAGAA CCAACCGTTC GATCATGCAG CACACAACCA 
TGCACAATGT GTGATCGTAC TTACACTGCC CTCAGTGGAG CGCTGTCATC ACCATATTGG 
CCTGATAAAT CATCAATACC CAGGTACTGT CGGCTTGTGA TAGATATTGA ACCTGATAAG 
AGGATCTTGT TGGCCTTCCA TGAGTTTACC CTCGAGGGTA TAGATCTGTT TAGTCCCTCC 
AAGACTGAAG AAGATGTCTG CTTGTGTGGT GTGCTCGTGG AGATACGGGA TGATGGTGGG 
CGCATGAATC GTTACTGTGG AACCAAGAAA CCGTTTGCTT GGGTCTCTGA GACGGATACA 
GTAACGCTGG AATTTATCAC ACCCTATTCT CTCGTCTTTA ATCGCTTCCT TGCATCTTAT 
ACCACCATCG ATACAAAACC AAGTTGCTAT CGCTTCATTG ACCTGACCGG CCTCCATACC 
GGTCTCGAGG CAGATGGAAT CACTGAGGTT CCTTTGCCTC AAAAAGGCTT CCTGCACTCG 
TTGAACTTCC CGAACGCCTA TCCCGACAAC CAGAACTGCG AGTACGTTCT CCATGGCAAT 
CCAGAGCATC GCATCGTGCT GTACTTTGAC GAGTTTGAGC TAGAACCTGG TCCGGCATGC 
GAGGCTGACT ATGTCAAGAT TGAGGATTTC TACAGTCGGC GCTCTAACGT CTACTGCGGC 
AAACATCACT CTTTCTCCTG GATCTCTGAT AGCAATGAAG TCTGGATCAC GTTCAGATCC 
AACGCCAATC AAGTCTACAC CGGTTTCCTT CTGGCCTACG AACTCATGGA TCGGGAAGGA 
AATGAGACGA TCGCGCTAGA AGAAGAGTCC GGTTTCTTTG TGTCGCCTGG TTACCCTCTG 
ATCTACGAGT CGGACGCGTC GGCGGAATAC GCCATCCGCA CTGACCCTAG CAAGCTCCTC 
GTCGTGACCT TCCACGACTT CCAGCTGGAG AGAGACGACT ACCCCGCTCC GCTGCCTGTG 
GGGATTCTGG AGATGGACAA GCGAAGCACG TTGAACGAGC ATCAGAATCA CTACGGCGAC 
CGATACGACT TATGTGTTGA TTACGTCAAG ATCATCGACA TTTTCGACAA TCATGTGGAA 
ACGTACTGTG GGCGGATCCC GGTGTTTAGC TATATCTCCC GTGGCAACGA GATCCTGGTC 
CAGTTTAAGA CCGATCTGAA CATGAACTAC AGAGGCTTCA ACGCGTCTTA CAGGTCCATC 
TCTAGAAGAA CACCAAATGA TTGGGAACTT GTCTACCGAG TGGCAAAAGG AGGCGAAACC 
GAGGCTCTTG ATAAGTTCCT GATGCAGGAT CGGGGCTACA GCGAGTCTGA CCCCAATGCA 
AAGGGAACCA CAATTACTCG CTCTCTTGCT ACCTTCAAGG ATTCAGATGT CCCAAATTGG 
GAGAATATCG GAATCAGAAA AGTCAAAGTT GACGCCTATA AGTCGGGAAG CATCATAGCA 
ACTTTCCGCT TTGATGGCCA TGGCACAAGT CTCAGTGACT GGTTTAGTTG CGAGAAGATG 
ACCTACAGCG GTTACGACGA CCTCAACTCA AAAGATTGTG GTGGTCTCGT GTTTGGCCTT 
ACAGGAATGC CGCCGGAGTT TGAAATCACA ACAACAATTG CCAACGGCAA GACGTGCGAT 
GATGACATGG ATCATAGGGT GTGGCTCGAT GTCCAAGATG TTGCGGAAGA GGGGGATTGT 
ACATACGAGG AGCCGGAAAT TGATATCAAC TCCATCATCT TCGTTAGCAG CGCTACGACG 
GGAGGGGAAA CTGCAACGGG AGCAAACATA GCCGAAGCAG ACGTGTTCAC CATCTGGGTT 
CAAAAAGATT GCGATGAAAG AATCTCGCCA GCCAGGGACA GCCCTGTAAA CATTGCTTCC 
AAGGGGCACG ATGAGCTTGA CTACAACAAT AACGAGTATT GCGATCTCTA TCTGAGTGTC 
CATCCGTTTG ATCGGCTCGC GATTGAATTC ACCGATTTTG AGCTGGAAGA AGATGAGTGT 
CTCGCAAATG ATTACGTCCA GATTGAGAAC ATAGAAACGG CCTTTGCGCA GATCTTCTGT 
GGTGTTCAAC ACCAAGTAGT ATGGACCGCT ACTGCGAATG CTGCCATCTT GAGGTTCAGA 
ACGGACAGTG TGGTAACGTA CAATGGTTGG GGTGCCAGTG TGAGAGCAGA ACGGCGAAAT 
TTGGAAGATT GTTCCACCGT CACCGTGAAC CCAACCGGCA ACGTGACCAA TCCCGATTAC 
CCCAACTCAC TCCAGGATTA TCGCTACTAC GCCTGCGAGT TCATCGTACA CGCCCATCCG 
GAGGACCGAG TTATCTTCTC ATTCTCGCGT TTCATCTTTG AGACAGAGAA CCCACCAGAG 
TTGCAGATTG ACCCAAGGTC AACCCGGGCC AACTTCATCG ATGTGTCGGG TCGTCTCTAC 
CCCTTCTCTT GGACCTCTGA TACCAACGAG CTAGCGCTTA TCTACCACGC CCCTCTTCCC 
TACCGGACCT ACATATTCTT TGAGGGCGAC TACCGCACCC TAGAGGCTCC TCGACCGGCC 
ACCTGCCAAC GGTTTGCGGA TGCCGATCTG GGAGGGACGT TCTCCTGGCC GACGGAATCT 
CTGTCGACCG GTCTGCAGAA CTGTTACGTG ATCGTGAGGT CCGACCCAGT CGAGCATAGA 
CGAGTCCAGA TAATCTTTGA CAATCTTAAG ATGCCCCAGT CGGATTGCAA TAGCTACATC 
AATACTCTTG ATACCACTAC AAGGCGTAAC GACATGTATT GTCAGAGAAT CCGCATCCCC 
TTCGCATGGA CCTCAGATGC CAATGAGGTC GTGATTAAAT TTTTCCATGG GCCGACCTTC 
GCTCTCCACC AGGTCAACGT ACTTTACAGG AGCCGAGCAA GGGTTCGTCC TGAAGATGCC 
AAAGTGTTGA CGGCTCTGAC GGGCGGTGAC TTCAGCAGCC CCAACTACCC CCAACCCTAC 
GATTCACAAT CCAAGAAGGA GTACCTCTTG CACGCCCCTG CAGAACATCT CGTCATTGTT 
GTCTTCAAAG ACTTCTCGGT TGAAGGTGCC ATAGAACCAA GTCCTCTTAT AGAGCCTGTA 
TTAAGGCCTG GCCGTAGCGT GGAGGACCTC TCCGCTCACT ACAAACACAT GTACAAGAGG 
CAGACGATAG TAGAGCCACC TACACCTGAA TGCCGCTACG ACTATGTTTC GGTTGAAGAC 
GTACTGGTGA ACACGGAGAC CAAGTACTGC GGGGATCAGC AACCGTTTGG TGTGGCGACT 
CAGGGCAACG AGGCCCTAGT CCTGTTCCTG ACTGATGAGA CCGTTGAGAA ACGAGGATTC 
ACCGCTCTCT ATGCTTTTGA GCCTCGACCG GCTATATCAG ACTGCGGTAC GCTGCAAACC 
GCGGACCAGG GCATCCTCCA GTCTCCGAAC TACCCTAACT ACTACCCGGA CGGGGCATAC 
TGCCGAGACG TGATACAGGT GGACCCAAAG AACCGCATCG TTATCGACTT TCGTTTCTTG 
AACTTGGAGG ATGATATCAC CTGCAGTAAA GACTACCTTG AGGTGCGTGA TGTAGTAACG 
GGGAGAGCGG AGAGGTACTG TGGACACAAG GCTACAGCCT TCCAATGGAA GTCGGACACC 
AACCTGGTCT TCCTGACGTT CGTCAGCGAC AATGCCAACA GCGATCCTGG ATACGATGCA 
GGATACACCA TAGAGGATAG ATCAGATGAA CCTACCTGCG TGCACACCAT CACGGCAACG 
TCCGGTACCA TCCACTCGCC GAGGTTCCCT CGAGACTACC CCAACCAGCT CAGGTGCGAG 
TACAACATCA AGGTGTATCC GGATTATGGA ATCAAGCTCA CCTTCCAAGA GTTCAACCTG 
GAGCCCCCCG CCCCGGGAAG CACGGACTGT GCCTTTGATT ATCTACAGAT TATTGACCCG 
ACGACGGTCA ACGTCGTGAC AGCCGAGAAG CTCTGCGGCT CCAAGAACAC TTTTGAACGT 
CAGATCCAGT TTAATGAAGT CAGTCTCATC TTTGTCAGTG ATTTCATCAC GTTCTACAAG 
GGTTTCCTGG TTGATTTCCA ACAGATCTCG CTCGGACAAA CCCCACAAAC CGTCCAAACT 
ACTAGTTGCA ATGAGCAGCT TACTGCTCCG TCAGGAACCT TCTCTAGCCC TGGTTACCCC 
GGTAACTATC CTGACAACTC CTTGTGCGTC ACCCTCATCA CTGTTGCCGT GGGTTACCAT 
GTCAGAATCA ACTTCAATGA CTTCAATACA GAAGGCTATA CAAGCTGCCC GTATGATTAT 
GTTCTGGTAC AAGATGTTCA GGTTCCTGGT CTGGACGAGA GGTTCTGCGG GCGCAAGCAG 
CACCCTTTCT CGTGGACCTC GGAAAGTAAC GTGGTTTCAG TGACGTTCAA GAGTGACGAT 
AGGACACCCT CGTCCGGTTT CTATGCAGCG TACGAATCAG CGCCGAACGT AGCCGCCGCC 
GTCGTTGCCC CAGTCACAGG TTGTGAGACG CACTACGGCA TCACCGAAAT CAGCGGAACT 
TCAGGAACCG TGCAGTCTCC GGACTATCCT AGCCTCTATC CACTCAATGT TGACTGCTTC 
GTCCGCTTCA CGACCGGTTA CGGATCTCGA TGCCAAATCA ACTTCATCGT CTTCAACCTG 
GAGGCCTCAA GCACATGCAG TTACGACTAT GTAAAGGTAA CGAGTATTCC AGATGGCGCA 
ACCCAGACGT TCTGCGGGTC TCTTGCTCCA TTTGAATGGC ACTCCCATTC TGCTACATGC 
GAGATCCAGT TCCACAGCGA CTCCTACCAA AGAGCATCGG GCTACCATGC ACAAATCACA 
GCTGTTTCTT CAAATATCCA TGCACCCTAC CAATCGCATC AACCTCCAAA TGAACCAGCT 
TCACCGTACC AACCACCAAA TGAACCTCCG GCACCATACC AGCCACACGC ACCGTACCAA 
CCACCAAATG AACCGCCTGC ACCGTACCAA CCAGCTGCAC CGTACCATGC ACCACCAAAT 
GAACCTCCGG CACCATACCA GCCACACGCA CCCTACCAAC CACCAAATGA ACCTCCCGCA 
CCATACCAGC CACATGCACC GTACCAACCA CCAAATGAAC CTCCGGCACC ATACCAGCCA 
CATGCACCGT ACCAACCACC AAATGAACCT CCTGCACCGT ACCAACCGTA TCAACCTCCA 
AATGAACCGC CTGCACCATA CCAACCACCT GCACCGTACC AACCACCAAA TGAACCTCCG 
CCACCATACC AACCACATGC ACCGTACCAA CCTCCAAATG AACCTCCGGC ACCGTACCAA 
CCACATGCAC CGTACCCACC ACCAAATGAA CCACCAGCAC CATACCAACC ACCACATGGG 
CCTCCTGCAC CATACCAACC ACCAGCTGAA GCAACAGCAA TAGCCGCAGA GTGCACCCAA 
GGTCTCCAAG CCGTGGTAGG GGAGAAGAAG TCCTTTGCTT CCCCTGGCTG GCCCGTCAGG 
TACCCCCTCC AGGTGCAGTG CTCGACCACG ATCACTGCCA GTGCCAACGG CAGGATAAGG 
ATAGAGTTCA CTCACTTTGA ATTGGAAGGA GACGAATGCA AATATGACTA TGTGCAGATA 
TCGGAACCCT ACCAGAGTGG CACACCTGAG AAGCTGTGCG GCGTCAAAAC TCCGTTCGTC 
TATGAGAGCA AGTCTGGCCG CGTGGTTGTA ACACTCGTCA GCGATGGTTA CGTGACGAAA 
AAGGGCTTCC TCGCAACATT TACGCAATTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030845101.1
CDS	99..6131
Translation	MWNRISGHALWLCTQHVLLVVLLLGCCHLPSVESDPTLTHRWAVTYGECSQTCGGGTQILVKSCYTLATNSQPSQTAPISYCSGLDHPPEPTVRSCSTQPCTMCDRTYTALSGALSSPYWPDKSSIPRYCRLVIDIEPDKRILLAFHEFTLEGIDLFSPSKTEEDVCLCGVLVEIRDDGGRMNRYCGTKKPFAWVSETDTVTLEFITPYSLVFNRFLASYTTIDTKPSCYRFIDLTGLHTGLEADGITEVPLPQKGFLHSLNFPNAYPDNQNCEYVLHGNPEHRIVLYFDEFELEPGPACEADYVKIEDFYSRRSNVYCGKHHSFSWISDSNEVWITFRSNANQVYTGFLLAYELMDREGNETIALEEESGFFVSPGYPLIYESDASAEYAIRTDPSKLLVVTFHDFQLERDDYPAPLPVGILEMDKRSTLNEHQNHYGDRYDLCVDYVKIIDIFDNHVETYCGRIPVFSYISRGNEILVQFKTDLNMNYRGFNASYRSISRRTPNDWELVYRVAKGGETEALDKFLMQDRGYSESDPNAKGTTITRSLATFKDSDVPNWENIGIRKVKVDAYKSGSIIATFRFDGHGTSLSDWFSCEKMTYSGYDDLNSKDCGGLVFGLTGMPPEFEITTTIANGKTCDDDMDHRVWLDVQDVAEEGDCTYEEPEIDINSIIFVSSATTGGETATGANIAEADVFTIWVQKDCDERISPARDSPVNIASKGHDELDYNNNEYCDLYLSVHPFDRLAIEFTDFELEEDECLANDYVQIENIETAFAQIFCGVQHQVVWTATANAAILRFRTDSVVTYNGWGASVRAERRNLEDCSTVTVNPTGNVTNPDYPNSLQDYRYYACEFIVHAHPEDRVIFSFSRFIFETENPPELQIDPRSTRANFIDVSGRLYPFSWTSDTNELALIYHAPLPYRTYIFFEGDYRTLEAPRPATCQRFADADLGGTFSWPTESLSTGLQNCYVIVRSDPVEHRRVQIIFDNLKMPQSDCNSYINTLDTTTRRNDMYCQRIRIPFAWTSDANEVVIKFFHGPTFALHQVNVLYRSRARVRPEDAKVLTALTGGDFSSPNYPQPYDSQSKKEYLLHAPAEHLVIVVFKDFSVEGAIEPSPLIEPVLRPGRSVEDLSAHYKHMYKRQTIVEPPTPECRYDYVSVEDVLVNTETKYCGDQQPFGVATQGNEALVLFLTDETVEKRGFTALYAFEPRPAISDCGTLQTADQGILQSPNYPNYYPDGAYCRDVIQVDPKNRIVIDFRFLNLEDDITCSKDYLEVRDVVTGRAERYCGHKATAFQWKSDTNLVFLTFVSDNANSDPGYDAGYTIEDRSDEPTCVHTITATSGTIHSPRFPRDYPNQLRCEYNIKVYPDYGIKLTFQEFNLEPPAPGSTDCAFDYLQIIDPTTVNVVTAEKLCGSKNTFERQIQFNEVSLIFVSDFITFYKGFLVDFQQISLGQTPQTVQTTSCNEQLTAPSGTFSSPGYPGNYPDNSLCVTLITVAVGYHVRINFNDFNTEGYTSCPYDYVLVQDVQVPGLDERFCGRKQHPFSWTSESNVVSVTFKSDDRTPSSGFYAAYESAPNVAAAVVAPVTGCETHYGITEISGTSGTVQSPDYPSLYPLNVDCFVRFTTGYGSRCQINFIVFNLEASSTCSYDYVKVTSIPDGATQTFCGSLAPFEWHSHSATCEIQFHSDSYQRASGYHAQITAVSSNIHAPYQSHQPPNEPASPYQPPNEPPAPYQPHAPYQPPNEPPAPYQPAAPYHAPPNEPPAPYQPHAPYQPPNEPPAPYQPHAPYQPPNEPPAPYQPHAPYQPPNEPPAPYQPYQPPNEPPAPYQPPAPYQPPNEPPPPYQPHAPYQPPNEPPAPYQPHAPYPPPNEPPAPYQPPHGPPAPYQPPAEATAIAAECTQGLQAVVGEKKSFASPGWPVRYPLQVQCSTTITASANGRIRIEFTHFELEGDECKYDYVQISEPYQSGTPEKLCGVKTPFVYESKSGRVVVTLVSDGYVTKKGFLATFTQF

Target ORF information:

RefSeq Version	XM_030989241.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus GTP-binding protein (mgb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030989241.1

ORF Insert Sequence:

ATGTGGAACC GTATCAGTGG ACACGCCCTT TGGTTATGCA CTCAGCACGT TTTATTAGTT 
GTCTTATTAC TGGGATGCTG CCATCTACCG TCGGTTGAAT CGGACCCAAC CCTGACGCAT 
AGATGGGCGG TTACATATGG AGAATGTAGT CAGACATGCG GTGGAGGTAC ACAAATCCTT 
GTCAAAAGCT GTTACACATT GGCCACGAAC TCACAGCCCT CACAGACGGC ACCCATTAGC 
TACTGCAGTG GACTGGACCA CCCACCAGAA CCAACCGTTC GATCATGCAG CACACAACCA 
TGCACAATGT GTGATCGTAC TTACACTGCC CTCAGTGGAG CGCTGTCATC ACCATATTGG 
CCTGATAAAT CATCAATACC CAGGTACTGT CGGCTTGTGA TAGATATTGA ACCTGATAAG 
AGGATCTTGT TGGCCTTCCA TGAGTTTACC CTCGAGGGTA TAGATCTGTT TAGTCCCTCC 
AAGACTGAAG AAGATGTCTG CTTGTGTGGT GTGCTCGTGG AGATACGGGA TGATGGTGGG 
CGCATGAATC GTTACTGTGG AACCAAGAAA CCGTTTGCTT GGGTCTCTGA GACGGATACA 
GTAACGCTGG AATTTATCAC ACCCTATTCT CTCGTCTTTA ATCGCTTCCT TGCATCTTAT 
ACCACCATCG ATACAAAACC AAGTTGCTAT CGCTTCATTG ACCTGACCGG CCTCCATACC 
GGTCTCGAGG CAGATGGAAT CACTGAGGTT CCTTTGCCTC AAAAAGGCTT CCTGCACTCG 
TTGAACTTCC CGAACGCCTA TCCCGACAAC CAGAACTGCG AGTACGTTCT CCATGGCAAT 
CCAGAGCATC GCATCGTGCT GTACTTTGAC GAGTTTGAGC TAGAACCTGG TCCGGCATGC 
GAGGCTGACT ATGTCAAGAT TGAGGATTTC TACAGTCGGC GCTCTAACGT CTACTGCGGC 
AAACATCACT CTTTCTCCTG GATCTCTGAT AGCAATGAAG TCTGGATCAC GTTCAGATCC 
AACGCCAATC AAGTCTACAC CGGTTTCCTT CTGGCCTACG AACTCATGGA TCGGGAAGGA 
AATGAGACGA TCGCGCTAGA AGAAGAGTCC GGTTTCTTTG TGTCGCCTGG TTACCCTCTG 
ATCTACGAGT CGGACGCGTC GGCGGAATAC GCCATCCGCA CTGACCCTAG CAAGCTCCTC 
GTCGTGACCT TCCACGACTT CCAGCTGGAG AGAGACGACT ACCCCGCTCC GCTGCCTGTG 
GGGATTCTGG AGATGGACAA GCGAAGCACG TTGAACGAGC ATCAGAATCA CTACGGCGAC 
CGATACGACT TATGTGTTGA TTACGTCAAG ATCATCGACA TTTTCGACAA TCATGTGGAA 
ACGTACTGTG GGCGGATCCC GGTGTTTAGC TATATCTCCC GTGGCAACGA GATCCTGGTC 
CAGTTTAAGA CCGATCTGAA CATGAACTAC AGAGGCTTCA ACGCGTCTTA CAGGTCCATC 
TCTAGAAGAA CACCAAATGA TTGGGAACTT GTCTACCGAG TGGCAAAAGG AGGCGAAACC 
GAGGCTCTTG ATAAGTTCCT GATGCAGGAT CGGGGCTACA GCGAGTCTGA CCCCAATGCA 
AAGGGAACCA CAATTACTCG CTCTCTTGCT ACCTTCAAGG ATTCAGATGT CCCAAATTGG 
GAGAATATCG GAATCAGAAA AGTCAAAGTT GACGCCTATA AGTCGGGAAG CATCATAGCA 
ACTTTCCGCT TTGATGGCCA TGGCACAAGT CTCAGTGACT GGTTTAGTTG CGAGAAGATG 
ACCTACAGCG GTTACGACGA CCTCAACTCA AAAGATTGTG GTGGTCTCGT GTTTGGCCTT 
ACAGGAATGC CGCCGGAGTT TGAAATCACA ACAACAATTG CCAACGGCAA GACGTGCGAT 
GATGACATGG ATCATAGGGT GTGGCTCGAT GTCCAAGATG TTGCGGAAGA GGGGGATTGT 
ACATACGAGG AGCCGGAAAT TGATATCAAC TCCATCATCT TCGTTAGCAG CGCTACGACG 
GGAGGGGAAA CTGCAACGGG AGCAAACATA GCCGAAGCAG ACGTGTTCAC CATCTGGGTT 
CAAAAAGATT GCGATGAAAG AATCTCGCCA GCCAGGGACA GCCCTGTAAA CATTGCTTCC 
AAGGGGCACG ATGAGCTTGA CTACAACAAT AACGAGTATT GCGATCTCTA TCTGAGTGTC 
CATCCGTTTG ATCGGCTCGC GATTGAATTC ACCGATTTTG AGCTGGAAGA AGATGAGTGT 
CTCGCAAATG ATTACGTCCA GATTGAGAAC ATAGAAACGG CCTTTGCGCA GATCTTCTGT 
GGTGTTCAAC ACCAAGTAGT ATGGACCGCT ACTGCGAATG CTGCCATCTT GAGGTTCAGA 
ACGGACAGTG TGGTAACGTA CAATGGTTGG GGTGCCAGTG TGAGAGCAGA ACGGCGAAAT 
TTGGAAGATT GTTCCACCGT CACCGTGAAC CCAACCGGCA ACGTGACCAA TCCCGATTAC 
CCCAACTCAC TCCAGGATTA TCGCTACTAC GCCTGCGAGT TCATCGTACA CGCCCATCCG 
GAGGACCGAG TTATCTTCTC ATTCTCGCGT TTCATCTTTG AGACAGAGAA CCCACCAGAG 
TTGCAGATTG ACCCAAGGTC AACCCGGGCC AACTTCATCG ATGTGTCGGG TCGTCTCTAC 
CCCTTCTCTT GGACCTCTGA TACCAACGAG CTAGCGCTTA TCTACCACGC CCCTCTTCCC 
TACCGGACCT ACATATTCTT TGAGGGCGAC TACCGCACCC TAGAGGCTCC TCGACCGGCC 
ACCTGCCAAC GGTTTGCGGA TGCCGATCTG GGAGGGACGT TCTCCTGGCC GACGGAATCT 
CTGTCGACCG GTCTGCAGAA CTGTTACGTG ATCGTGAGGT CCGACCCAGT CGAGCATAGA 
CGAGTCCAGA TAATCTTTGA CAATCTTAAG ATGCCCCAGT CGGATTGCAA TAGCTACATC 
AATACTCTTG ATACCACTAC AAGGCGTAAC GACATGTATT GTCAGAGAAT CCGCATCCCC 
TTCGCATGGA CCTCAGATGC CAATGAGGTC GTGATTAAAT TTTTCCATGG GCCGACCTTC 
GCTCTCCACC AGGTCAACGT ACTTTACAGG AGCCGAGCAA GGGTTCGTCC TGAAGATGCC 
AAAGTGTTGA CGGCTCTGAC GGGCGGTGAC TTCAGCAGCC CCAACTACCC CCAACCCTAC 
GATTCACAAT CCAAGAAGGA GTACCTCTTG CACGCCCCTG CAGAACATCT CGTCATTGTT 
GTCTTCAAAG ACTTCTCGGT TGAAGGTGCC ATAGAACCAA GTCCTCTTAT AGAGCCTGTA 
TTAAGGCCTG GCCGTAGCGT GGAGGACCTC TCCGCTCACT ACAAACACAT GTACAAGAGG 
CAGACGATAG TAGAGCCACC TACACCTGAA TGCCGCTACG ACTATGTTTC GGTTGAAGAC 
GTACTGGTGA ACACGGAGAC CAAGTACTGC GGGGATCAGC AACCGTTTGG TGTGGCGACT 
CAGGGCAACG AGGCCCTAGT CCTGTTCCTG ACTGATGAGA CCGTTGAGAA ACGAGGATTC 
ACCGCTCTCT ATGCTTTTGA GCCTCGACCG GCTATATCAG ACTGCGGTAC GCTGCAAACC 
GCGGACCAGG GCATCCTCCA GTCTCCGAAC TACCCTAACT ACTACCCGGA CGGGGCATAC 
TGCCGAGACG TGATACAGGT GGACCCAAAG AACCGCATCG TTATCGACTT TCGTTTCTTG 
AACTTGGAGG ATGATATCAC CTGCAGTAAA GACTACCTTG AGGTGCGTGA TGTAGTAACG 
GGGAGAGCGG AGAGGTACTG TGGACACAAG GCTACAGCCT TCCAATGGAA GTCGGACACC 
AACCTGGTCT TCCTGACGTT CGTCAGCGAC AATGCCAACA GCGATCCTGG ATACGATGCA 
GGATACACCA TAGAGGATAG ATCAGATGAA CCTACCTGCG TGCACACCAT CACGGCAACG 
TCCGGTACCA TCCACTCGCC GAGGTTCCCT CGAGACTACC CCAACCAGCT CAGGTGCGAG 
TACAACATCA AGGTGTATCC GGATTATGGA ATCAAGCTCA CCTTCCAAGA GTTCAACCTG 
GAGCCCCCCG CCCCGGGAAG CACGGACTGT GCCTTTGATT ATCTACAGAT TATTGACCCG 
ACGACGGTCA ACGTCGTGAC AGCCGAGAAG CTCTGCGGCT CCAAGAACAC TTTTGAACGT 
CAGATCCAGT TTAATGAAGT CAGTCTCATC TTTGTCAGTG ATTTCATCAC GTTCTACAAG 
GGTTTCCTGG TTGATTTCCA ACAGATCTCG CTCGGACAAA CCCCACAAAC CGTCCAAACT 
ACTAGTTGCA ATGAGCAGCT TACTGCTCCG TCAGGAACCT TCTCTAGCCC TGGTTACCCC 
GGTAACTATC CTGACAACTC CTTGTGCGTC ACCCTCATCA CTGTTGCCGT GGGTTACCAT 
GTCAGAATCA ACTTCAATGA CTTCAATACA GAAGGCTATA CAAGCTGCCC GTATGATTAT 
GTTCTGGTAC AAGATGTTCA GGTTCCTGGT CTGGACGAGA GGTTCTGCGG GCGCAAGCAG 
CACCCTTTCT CGTGGACCTC GGAAAGTAAC GTGGTTTCAG TGACGTTCAA GAGTGACGAT 
AGGACACCCT CGTCCGGTTT CTATGCAGCG TACGAATCAG CGCCGAACGT AGCCGCCGCC 
GTCGTTGCCC CAGTCACAGG TTGTGAGACG CACTACGGCA TCACCGAAAT CAGCGGAACT 
TCAGGAACCG TGCAGTCTCC GGACTATCCT AGCCTCTATC CACTCAATGT TGACTGCTTC 
GTCCGCTTCA CGACCGGTTA CGGATCTCGA TGCCAAATCA ACTTCATCGT CTTCAACCTG 
GAGGCCTCAA GCACATGCAG TTACGACTAT GTAAAGGTAA CGAGTATTCC AGATGGCGCA 
ACCCAGACGT TCTGCGGGTC TCTTGCTCCA TTTGAATGGC ACTCCCATTC TGCTACATGC 
GAGATCCAGT TCCACAGCGA CTCCTACCAA AGAGCATCGG GCTACCATGC ACAAATCACA 
GCTGTTTCTT CAAATATCCA TGCACCCTAC CAATCGCATC AACCTCCAAA TGAACCAGCT 
TCACCGTACC AACCACCAAA TGAACCTCCG GCACCATACC AGCCACACGC ACCGTACCAA 
CCACCAAATG AACCGCCTGC ACCGTACCAA CCAGCTGCAC CGTACCATGC ACCACCAAAT 
GAACCTCCGG CACCATACCA GCCACACGCA CCCTACCAAC CACCAAATGA ACCTCCCGCA 
CCATACCAGC CACATGCACC GTACCAACCA CCAAATGAAC CTCCGGCACC ATACCAGCCA 
CATGCACCGT ACCAACCACC AAATGAACCT CCTGCACCGT ACCAACCGTA TCAACCTCCA 
AATGAACCGC CTGCACCATA CCAACCACCT GCACCGTACC AACCACCAAA TGAACCTCCG 
CCACCATACC AACCACATGC ACCGTACCAA CCTCCAAATG AACCTCCGGC ACCGTACCAA 
CCACATGCAC CGTACCCACC ACCAAATGAA CCACCAGCAC CATACCAACC ACCACATGGG 
CCTCCTGCAC CATACCAACC ACCAGCTGAA GCAACAGCAA TAGCCGCAGA GTGCACCCAA 
GGTCTCCAAG CCGTGGTAGG GGAGAAGAAG TCCTTTGCTT CCCCTGGCTG GCCCGTCAGG 
TACCCCCTCC AGGTGCAGTG CTCGACCACG ATCACTGCCA GTGCCAACGG CAGGATAAGG 
ATAGAGTTCA CTCACTTTGA ATTGGAAGGA GACGAATGCA AATATGACTA TGTGCAGATA 
TCGGAACCCT ACCAGAGTGG CACACCTGAG AAGCTGTGCG GCGTCAAAAC TCCGTTCGTC 
TATGAGAGCA AGTCTGGCCG CGTGGTTGTA ACACTCGTCA GCGATGGTTA CGTGACGAAA 
AAGGGCTTCC TCGCAACATT TACGCAATTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Genome research22(10)2079-87(2012 Oct)
Tu Q,Cameron RA,Worley KC,Gibbs RA,Davidson EH

Rendezvin: An essential gene encoding independent, differentially secreted egg proteins that organize the fertilization envelope proteome after self-association.
Molecular biology of the cell17(12)5241-52(2006 Dec)
Wong JL,Wessel GM

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mgb cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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