LOC579713 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC579713
Entrez Gene ID	579713
Full Name	codanin-1
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011664737.2	XP_011663039.2	codanin-1 isoform X1
XM_011664739.1	XP_011663041.1	codanin-1 isoform X3
XM_011664739.2	XP_011663041.2	codanin-1 isoform X2
XM_011664737.1	XP_011663039.1	codanin-1 isoform X1
XM_011664738.1	XP_011663040.1	codanin-1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC579713 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC579713 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd22841	XM_011664737.1 Latest version!	Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSd22842	XM_011664738.1 Latest version!	Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSd22843	XM_011664739.1 Latest version!	Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSd65759	XM_011664737.2 Latest version!	Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSd65760	XM_011664739.2 Latest version!	Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC579713 ( XM_011664737.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011663039.1 Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd22841
Clone ID Related Accession (Same CDS sequence)	XM_011664737.1
Accession Version	XM_011664737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	codanin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011998944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGA TATTGGAACA CATTTTGTCA ACAAAATTGT CAATACAAGA AGTGTTGGAG 
TGGTCAGTCG AAGGGAAGGT TGAAGTGAGT AACTCTGGAT TTGCAGACTT GTTGAGGGAT 
ACCAAACTGC AAACAGAATT TCTACCATTC TTCATCAATA GCTTGCGGGA TCAGACAGCC 
CACTTAGTAT GCAGGGGATC ATCTGCTGTG TCTTCCCCAG CGAGGACGCC AAGCAGCTTA 
CAAAAACTAA AAAGAGCCAA TCAGACACGC CCTCAGCGCA GTGGAGGTTC AAAGGTCAAT 
CGTACACAGC TGTTTGCTGC TGATAACAGC AAGTCCAGCT GGTCAGACAA AGACTTTACA 
CCCGTTTCAA GACTAACAGC AGTAGAGCAG TCGCCGCACA CTCCATCGTC ATCTAAGACA 
AATGGAAGAC ACGGAAGAAA TGTGTCTTGG AGTGGTGGTG GCGGTGATGC TGGCTCCAGC 
TCCAGGAGGA AGACTCCCGG ATCAGGATCT AGGAAGGATG AATCCATAAC GCTGGCTCAG 
TTCTTGGTGA CTCCCGAAAG CCAGTCAAGA GGAAAGGGCA GAAAGAGTCC TTTTGAAGAC 
TCTAACTGTG ATAGCTCATC GTCACATAAA AGCTCATCCA GTAGACGTCG CTCAGGAGGT 
AAAGGAAAGC AAACGGGACA GAATGAGAGC GGTGGCTCGA AAGGACAGCA TAAATTGAAA 
TACATTGACA AACCTGTGTT TGACTTGGAA AATGCCCAAG AATTCCCTCC AATGGGTGTA 
CCAAGAACTA TTACCCCAAG GAGGATAACT CCAACTGCAG TATCTTCCAG TAGTGGAGGC 
AGCCGGAGAG TCACCACACT GCTGTCCCCA GCCCCTTCTT GCCAGGGTCA AAATCAGGAA 
ACCAATGCCT TCAATCCCCC TGTAAACCAG ACTGCAGCAA CCAAGAGAGA CACTCATGAG 
TTGAAAAAAT CTTTGGAAAA CGAGAGGGAG TTGCTTAAGC AAGAAAAAAT GAAACAGAAA 
AATCAGCCAG AATGTAGTCC ATTCTCTCAA AATGCATTAG GACAGTCACC AAGAGGAATG 
TCTGTATTGC AATCTCCAAA GGGTATGACT CTAGCTCATA CCCCGACTAA GCTTCTGCGC 
AGTAATTCAG TTTCAAGCAT AACTGAGGCA ATACTACCTG ATGTGGAAAA AGTAACAGGG 
CATGACAAAC TGGATCTCAT AGCAGAGCTC TATGCATCAT TTCTACAAGA GAGCCTTGTT 
CCCAATTTGT CTGTGGAGTT GTACTTCCTG ATTCAGCTGT TGACTGCTAA AGGTAGCAAG 
CTTGATGAGG ATGAGGGAAT ACATTCACTG GAAGAGGACA TTGAAGACAT CAATTATCTC 
TCAACAATCC ACAACTGTGT TTACTTTGCT GCATCAGTGC TCAGCAGGCT GGTTGTAGTG 
TTATCTCTGC TGGATAAGAG CACCTTACGC CTCCTGGCGG ACAACCAAGT GGTCTCTCAG 
TTCAATCCCG AGCTGAAGAC CAGGCTTGAA CAGGTCACCA ATCATTGCCC AACGGAGTCT 
GGTCTTCAGG CTGCCATTAC ATCTCCCATG CAGGTTGTCC CGTTCTTGGC AGATACCGAC 
AACAGGCAGA ACTTTCCAAG TGACACTTCA TTCCACAGTT TCAAGAAACA GAGAGATGGT 
TTCTATAGTC TGATACGAGA CTGGGAAGAT AATCGCAGTA AACCTGGCTG GAAGATGCAG 
GAGAAACTAA CCCACAGGAT CAGGGATTTA ATGAAGCACA TGGATGTGAC AAACTACACC 
CACTTCTCAA GACTGTTCCA GTCACAACTG ATCAAGATGT GCCAAGGTGA TAGCTCAGTG 
GTTGATAGTG GAGATTCTCA GGAATTTAGC TTCATCCAGC AGCTGAGACG GACGAATCCA 
GGAAAGTTTA AAAGATTGGA GGAGCGTTTT TCAACTCCAG CTTCATCCGG TGGTCCATGC 
CCTCCTCCTT CGTTCCATGG ATGTCAGGAA TTCTTTAGAG ATTTCATCCT AGCTACAAAC 
AGTTACATAT TCAACCAGCA TTTGACGGAC AGCTTTGCTA CCAAGCTCAT TGAGCTTAAC 
GATTTACAGT TGCCAATCAC TGATCAGGTT GATATGACTT CTGTTGTTGA TGCGGAGGTA 
AGGGAAAGCT TCAGTTCATG TCTCCTAGCC CTGAGGGTCT TGTCCAAGTT CCTGGGATTT 
CTAGTCTTTC TACCTTACCA GGGTGACAAT AATCTTCCTG ATATCATGAA GGATAATGTT 
ATCATGATGA GGAGTAGGGC ATCTCTTCCC GTGGACATTC TTGGATGTTT GTCTGCATCA 
GAAACCAACC AGCGTCTGAT CCTGACCTTG CCCTGGGTCG TAGAGTATCT TAGTATGATA 
GATGGGCTGG CCTCTCAGAT ACCAGTTTAT CATCAAACAC TCAGTTTATT GGGGGATATT 
CACAGGAGGG TGTCTGTGCA ATGGTCTGAG AATCCTAGCA ACCATAATTC ACTTCTTATC 
TTGCTTCTCT TGGGATGGCT GTTCGAGAAC CCAGCTCTAC CAAGCAATCT ACATCTGACG 
AGTGGTATGA GCGTTGAGAG GGGTAGCCAT AGTAATAAAC CAGGATCTGA CCAATCTTTA 
GACCCCCTTC CTCTGGTGGA TAGGCTGGTT CTCTATACAT GCTGCCCTTT CCTAGGTGAT 
TTAAGAGGAC TGTTATCCGA GGCTAGTATG GGGGTGAACA ACAGGAATGC TCCGGTCAAG 
AAGATTACTC CTGTAGCAGT GGATGCACCT CAGACTGCCA TTATCACTCA GCACCAAATA 
CAGGTGGAAC TTGAGAAGAA CTTCTTCCAC ATCCACTCCG CTTCCCTCAA GAAGAGCGTG 
GAGTTTGTGA GCGGCAGGGT CGCCTCCAAC TGCATCAAGT CCATCGATGG CTCACTAGTC 
TCTGCAGCCA GGCTCCAGAT TCCAAAGCTA CTCACTGAAG TGCTGGTCTC GTCCAGCAGC 
AGCAAGAAAG AGGCTGGGGC TCTCACAGCC AAGGCTAAGG GAGTTGGAGA GAAGCTGAGC 
CTGGAGACGA CAGAGCTTGC CAGGAAAGAA GCCAAGGAGT TTATCAAGAA GAAGTGTAGG 
TCAGCTCTTG ATGTGTTATT ACCGTGTGAG ACATCCGAGG CAGTCCTGGA CACTGCTTGT 
GCTATCACCA CTAGACTTGC CTTAGAACGA ACAGAGAAAT GGATCACAAC ACAAATACCT 
AGTGCATTAC AACAGGAGGT GACATTGCAG CTACCCAAGT GCCTGAGAGC TCAGAGCACT 
CAACAGATGA AGAAAGAATC AACCAGTAGG AAAATACCAG ACAAGCCAGC CATGCACGAC 
AGACAGGGGG CTTGTCCTTC AACCGTCATT CAAGAACTCA AGGACGGTCT TCATAGAATT 
TTAATTGGAG ACACCGATGG AAACACAGCA GAGGGCATCT GTTCATCCAT CAATAACGTT 
CATATGATGA TCAACAACAG ACAGGACCTA ACACCATTGG CTTACCAGTC CATCCACCAA 
CTCACCATAG AGCTGTGTGT AAGCCTGGCT ATGAGACAAC CAACTCTGCT TCAGACCTCT 
GTCTGTACCT CACCTACAAT CTCCAAAGGG CAAAGGTCCA TCGTTCTACC ACAGGACAGT 
AATACACACG GCAAGAACTG TAGCAGCAGC TTACACAATA CAGAGAATGG CAAACACCAA 
GACAAGAACT TGATATGTTC AACACAGGGA GTAGTTGAAG ATGGAGAGGA GCAGTGCAAT 
AACCAAGAGA GTGGTTTACA CTCACATCTT GGGAGATATG GACAAGAACT TCAGTGCAAT 
ACAAAGGGAG GACAGGGTAC AGAGGAAGGA GCATTCACCA CTCCTGTTCA TAAACATGCA 
TGGAATGGTG ACCAAGGTGA AAGAGATGTA ACTAATGCAG AACAGACAGA AGGAGATGTG 
ACTTTACACC GGTCTAATAC GGATACACTC TTAGAGAGAC TGATGGGTTT GTGGAGGAAT 
GTTTTTTCTT TGAGTATTCC ACCTGGAGCT CTGTTGAGTG CAAGGACAGC ATGGATGATA 
TCACACAGTC ATAACCCACA GGTTTCCTGG CAGTGTCTCA TCCATATAGT GAAGTCCCTT 
CTTGCATGCA ATCTGGTCGG CATTGGGGCT GTCCAATCAG CTTTGAGCAA GATGATGATG 
TCATCATACA TTCCACCAAT TACAGAGGAT GTAGCAAGAA GCCTATTACC TCTGGTCACA 
GATAATGAAA TAGGTATAGA TCAGAATGCA TTGGACTTGC TGCATGCAGT TTGCTCCGAA 
GCAAGAGAGA ATGAAGATGC CTATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011663039.1
CDS	322..4671
Translation	MAVILEHILSTKLSIQEVLEWSVEGKVEVSNSGFADLLRDTKLQTEFLPFFINSLRDQTAHLVCRGSSAVSSPARTPSSLQKLKRANQTRPQRSGGSKVNRTQLFAADNSKSSWSDKDFTPVSRLTAVEQSPHTPSSSKTNGRHGRNVSWSGGGGDAGSSSRRKTPGSGSRKDESITLAQFLVTPESQSRGKGRKSPFEDSNCDSSSSHKSSSSRRRSGGKGKQTGQNESGGSKGQHKLKYIDKPVFDLENAQEFPPMGVPRTITPRRITPTAVSSSSGGSRRVTTLLSPAPSCQGQNQETNAFNPPVNQTAATKRDTHELKKSLENERELLKQEKMKQKNQPECSPFSQNALGQSPRGMSVLQSPKGMTLAHTPTKLLRSNSVSSITEAILPDVEKVTGHDKLDLIAELYASFLQESLVPNLSVELYFLIQLLTAKGSKLDEDEGIHSLEEDIEDINYLSTIHNCVYFAASVLSRLVVVLSLLDKSTLRLLADNQVVSQFNPELKTRLEQVTNHCPTESGLQAAITSPMQVVPFLADTDNRQNFPSDTSFHSFKKQRDGFYSLIRDWEDNRSKPGWKMQEKLTHRIRDLMKHMDVTNYTHFSRLFQSQLIKMCQGDSSVVDSGDSQEFSFIQQLRRTNPGKFKRLEERFSTPASSGGPCPPPSFHGCQEFFRDFILATNSYIFNQHLTDSFATKLIELNDLQLPITDQVDMTSVVDAEVRESFSSCLLALRVLSKFLGFLVFLPYQGDNNLPDIMKDNVIMMRSRASLPVDILGCLSASETNQRLILTLPWVVEYLSMIDGLASQIPVYHQTLSLLGDIHRRVSVQWSENPSNHNSLLILLLLGWLFENPALPSNLHLTSGMSVERGSHSNKPGSDQSLDPLPLVDRLVLYTCCPFLGDLRGLLSEASMGVNNRNAPVKKITPVAVDAPQTAIITQHQIQVELEKNFFHIHSASLKKSVEFVSGRVASNCIKSIDGSLVSAARLQIPKLLTEVLVSSSSSKKEAGALTAKAKGVGEKLSLETTELARKEAKEFIKKKCRSALDVLLPCETSEAVLDTACAITTRLALERTEKWITTQIPSALQQEVTLQLPKCLRAQSTQQMKKESTSRKIPDKPAMHDRQGACPSTVIQELKDGLHRILIGDTDGNTAEGICSSINNVHMMINNRQDLTPLAYQSIHQLTIELCVSLAMRQPTLLQTSVCTSPTISKGQRSIVLPQDSNTHGKNCSSSLHNTENGKHQDKNLICSTQGVVEDGEEQCNNQESGLHSHLGRYGQELQCNTKGGQGTEEGAFTTPVHKHAWNGDQGERDVTNAEQTEGDVTLHRSNTDTLLERLMGLWRNVFSLSIPPGALLSARTAWMISHSHNPQVSWQCLIHIVKSLLACNLVGIGAVQSALSKMMMSSYIPPITEDVARSLLPLVTDNEIGIDQNALDLLHAVCSEARENEDAYS

Target ORF information:

RefSeq Version	XM_011664737.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011664737.1

ORF Insert Sequence:

ATGGCGGTGA TATTGGAACA CATTTTGTCA ACAAAATTGT CAATACAAGA AGTGTTGGAG 
TGGTCAGTCG AAGGGAAGGT TGAAGTGAGT AACTCTGGAT TTGCAGACTT GTTGAGGGAT 
ACCAAACTGC AAACAGAATT TCTACCATTC TTCATCAATA GCTTGCGGGA TCAGACAGCC 
CACTTAGTAT GCAGGGGATC ATCTGCTGTG TCTTCCCCAG CGAGGACGCC AAGCAGCTTA 
CAAAAACTAA AAAGAGCCAA TCAGACACGC CCTCAGCGCA GTGGAGGTTC AAAGGTCAAT 
CGTACACAGC TGTTTGCTGC TGATAACAGC AAGTCCAGCT GGTCAGACAA AGACTTTACA 
CCCGTTTCAA GACTAACAGC AGTAGAGCAG TCGCCGCACA CTCCATCGTC ATCTAAGACA 
AATGGAAGAC ACGGAAGAAA TGTGTCTTGG AGTGGTGGTG GCGGTGATGC TGGCTCCAGC 
TCCAGGAGGA AGACTCCCGG ATCAGGATCT AGGAAGGATG AATCCATAAC GCTGGCTCAG 
TTCTTGGTGA CTCCCGAAAG CCAGTCAAGA GGAAAGGGCA GAAAGAGTCC TTTTGAAGAC 
TCTAACTGTG ATAGCTCATC GTCACATAAA AGCTCATCCA GTAGACGTCG CTCAGGAGGT 
AAAGGAAAGC AAACGGGACA GAATGAGAGC GGTGGCTCGA AAGGACAGCA TAAATTGAAA 
TACATTGACA AACCTGTGTT TGACTTGGAA AATGCCCAAG AATTCCCTCC AATGGGTGTA 
CCAAGAACTA TTACCCCAAG GAGGATAACT CCAACTGCAG TATCTTCCAG TAGTGGAGGC 
AGCCGGAGAG TCACCACACT GCTGTCCCCA GCCCCTTCTT GCCAGGGTCA AAATCAGGAA 
ACCAATGCCT TCAATCCCCC TGTAAACCAG ACTGCAGCAA CCAAGAGAGA CACTCATGAG 
TTGAAAAAAT CTTTGGAAAA CGAGAGGGAG TTGCTTAAGC AAGAAAAAAT GAAACAGAAA 
AATCAGCCAG AATGTAGTCC ATTCTCTCAA AATGCATTAG GACAGTCACC AAGAGGAATG 
TCTGTATTGC AATCTCCAAA GGGTATGACT CTAGCTCATA CCCCGACTAA GCTTCTGCGC 
AGTAATTCAG TTTCAAGCAT AACTGAGGCA ATACTACCTG ATGTGGAAAA AGTAACAGGG 
CATGACAAAC TGGATCTCAT AGCAGAGCTC TATGCATCAT TTCTACAAGA GAGCCTTGTT 
CCCAATTTGT CTGTGGAGTT GTACTTCCTG ATTCAGCTGT TGACTGCTAA AGGTAGCAAG 
CTTGATGAGG ATGAGGGAAT ACATTCACTG GAAGAGGACA TTGAAGACAT CAATTATCTC 
TCAACAATCC ACAACTGTGT TTACTTTGCT GCATCAGTGC TCAGCAGGCT GGTTGTAGTG 
TTATCTCTGC TGGATAAGAG CACCTTACGC CTCCTGGCGG ACAACCAAGT GGTCTCTCAG 
TTCAATCCCG AGCTGAAGAC CAGGCTTGAA CAGGTCACCA ATCATTGCCC AACGGAGTCT 
GGTCTTCAGG CTGCCATTAC ATCTCCCATG CAGGTTGTCC CGTTCTTGGC AGATACCGAC 
AACAGGCAGA ACTTTCCAAG TGACACTTCA TTCCACAGTT TCAAGAAACA GAGAGATGGT 
TTCTATAGTC TGATACGAGA CTGGGAAGAT AATCGCAGTA AACCTGGCTG GAAGATGCAG 
GAGAAACTAA CCCACAGGAT CAGGGATTTA ATGAAGCACA TGGATGTGAC AAACTACACC 
CACTTCTCAA GACTGTTCCA GTCACAACTG ATCAAGATGT GCCAAGGTGA TAGCTCAGTG 
GTTGATAGTG GAGATTCTCA GGAATTTAGC TTCATCCAGC AGCTGAGACG GACGAATCCA 
GGAAAGTTTA AAAGATTGGA GGAGCGTTTT TCAACTCCAG CTTCATCCGG TGGTCCATGC 
CCTCCTCCTT CGTTCCATGG ATGTCAGGAA TTCTTTAGAG ATTTCATCCT AGCTACAAAC 
AGTTACATAT TCAACCAGCA TTTGACGGAC AGCTTTGCTA CCAAGCTCAT TGAGCTTAAC 
GATTTACAGT TGCCAATCAC TGATCAGGTT GATATGACTT CTGTTGTTGA TGCGGAGGTA 
AGGGAAAGCT TCAGTTCATG TCTCCTAGCC CTGAGGGTCT TGTCCAAGTT CCTGGGATTT 
CTAGTCTTTC TACCTTACCA GGGTGACAAT AATCTTCCTG ATATCATGAA GGATAATGTT 
ATCATGATGA GGAGTAGGGC ATCTCTTCCC GTGGACATTC TTGGATGTTT GTCTGCATCA 
GAAACCAACC AGCGTCTGAT CCTGACCTTG CCCTGGGTCG TAGAGTATCT TAGTATGATA 
GATGGGCTGG CCTCTCAGAT ACCAGTTTAT CATCAAACAC TCAGTTTATT GGGGGATATT 
CACAGGAGGG TGTCTGTGCA ATGGTCTGAG AATCCTAGCA ACCATAATTC ACTTCTTATC 
TTGCTTCTCT TGGGATGGCT GTTCGAGAAC CCAGCTCTAC CAAGCAATCT ACATCTGACG 
AGTGGTATGA GCGTTGAGAG GGGTAGCCAT AGTAATAAAC CAGGATCTGA CCAATCTTTA 
GACCCCCTTC CTCTGGTGGA TAGGCTGGTT CTCTATACAT GCTGCCCTTT CCTAGGTGAT 
TTAAGAGGAC TGTTATCCGA GGCTAGTATG GGGGTGAACA ACAGGAATGC TCCGGTCAAG 
AAGATTACTC CTGTAGCAGT GGATGCACCT CAGACTGCCA TTATCACTCA GCACCAAATA 
CAGGTGGAAC TTGAGAAGAA CTTCTTCCAC ATCCACTCCG CTTCCCTCAA GAAGAGCGTG 
GAGTTTGTGA GCGGCAGGGT CGCCTCCAAC TGCATCAAGT CCATCGATGG CTCACTAGTC 
TCTGCAGCCA GGCTCCAGAT TCCAAAGCTA CTCACTGAAG TGCTGGTCTC GTCCAGCAGC 
AGCAAGAAAG AGGCTGGGGC TCTCACAGCC AAGGCTAAGG GAGTTGGAGA GAAGCTGAGC 
CTGGAGACGA CAGAGCTTGC CAGGAAAGAA GCCAAGGAGT TTATCAAGAA GAAGTGTAGG 
TCAGCTCTTG ATGTGTTATT ACCGTGTGAG ACATCCGAGG CAGTCCTGGA CACTGCTTGT 
GCTATCACCA CTAGACTTGC CTTAGAACGA ACAGAGAAAT GGATCACAAC ACAAATACCT 
AGTGCATTAC AACAGGAGGT GACATTGCAG CTACCCAAGT GCCTGAGAGC TCAGAGCACT 
CAACAGATGA AGAAAGAATC AACCAGTAGG AAAATACCAG ACAAGCCAGC CATGCACGAC 
AGACAGGGGG CTTGTCCTTC AACCGTCATT CAAGAACTCA AGGACGGTCT TCATAGAATT 
TTAATTGGAG ACACCGATGG AAACACAGCA GAGGGCATCT GTTCATCCAT CAATAACGTT 
CATATGATGA TCAACAACAG ACAGGACCTA ACACCATTGG CTTACCAGTC CATCCACCAA 
CTCACCATAG AGCTGTGTGT AAGCCTGGCT ATGAGACAAC CAACTCTGCT TCAGACCTCT 
GTCTGTACCT CACCTACAAT CTCCAAAGGG CAAAGGTCCA TCGTTCTACC ACAGGACAGT 
AATACACACG GCAAGAACTG TAGCAGCAGC TTACACAATA CAGAGAATGG CAAACACCAA 
GACAAGAACT TGATATGTTC AACACAGGGA GTAGTTGAAG ATGGAGAGGA GCAGTGCAAT 
AACCAAGAGA GTGGTTTACA CTCACATCTT GGGAGATATG GACAAGAACT TCAGTGCAAT 
ACAAAGGGAG GACAGGGTAC AGAGGAAGGA GCATTCACCA CTCCTGTTCA TAAACATGCA 
TGGAATGGTG ACCAAGGTGA AAGAGATGTA ACTAATGCAG AACAGACAGA AGGAGATGTG 
ACTTTACACC GGTCTAATAC GGATACACTC TTAGAGAGAC TGATGGGTTT GTGGAGGAAT 
GTTTTTTCTT TGAGTATTCC ACCTGGAGCT CTGTTGAGTG CAAGGACAGC ATGGATGATA 
TCACACAGTC ATAACCCACA GGTTTCCTGG CAGTGTCTCA TCCATATAGT GAAGTCCCTT 
CTTGCATGCA ATCTGGTCGG CATTGGGGCT GTCCAATCAG CTTTGAGCAA GATGATGATG 
TCATCATACA TTCCACCAAT TACAGAGGAT GTAGCAAGAA GCCTATTACC TCTGGTCACA 
GATAATGAAA TAGGTATAGA TCAGAATGCA TTGGACTTGC TGCATGCAGT TTGCTCCGAA 
GCAAGAGAGA ATGAAGATGC CTATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC579713 ( XM_011664738.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011663040.1 Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSd22842
Clone ID Related Accession (Same CDS sequence)	XM_011664738.1
Accession Version	XM_011664738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	codanin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011998944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGA TATTGGAACA CATTTTGTCA ACAAAATTGT CAATACAAGA AGTGTTGGAG 
TGGTCAGTCG AAGGGAAGGT TGAAGTGAGT AACTCTGGAT TTGCAGACTT GTTGAGGGAT 
ACCAAACTGC AAACAGAATT TCTACCATTC TTCATCAATA GCTTGCGGGA TCAGACAGCC 
CACTTAGTAT GCAGGGGATC ATCTGCTGTG TCTTCCCCAG CGAGGACGCC AAGCAGCTTA 
CAAAAACTAA AAAGAGCCAA TCAGACACGC CCTCAGCGCA GTGGAGGTTC AAAGGTCAAT 
CGTACACAGC TGTTTGCTGC TGATAACAGC AAGTCCAGCT GGTCAGACAA AGACTTTACA 
CCCGTTTCAA GACTAACAGC AGTAGAGCAG TCGCCGCACA CTCCATCGTC ATCTAAGACA 
AATGGAAGAC ACGGAAGAAA TGTGTCTTGG AGTGGTGGTG GCGGTGATGC TGGCTCCAGC 
TCCAGGAGGA AGACTCCCGG ATCAGGATCT AGGAAGGATG AATCCATAAC GCTGGCTCAG 
TTCTTGGTGA CTCCCGAAAG CCAGTCAAGA GGAAAGGGCA GAAAGAGTCC TTTTGAAGAC 
TCTAACTGTG ATAGCTCATC GTCACATAAA AGCTCATCCA GTAGACGTCG CTCAGGAGGT 
AAAGGAAAGC AAACGGGACA GAATGAGAGC GGTGGCTCGA AAGGACAGCA TAAATTGAAA 
TACATTGACA AACCTGTGTT TGACTTGGAA AATGCCCAAG AATTCCCTCC AATGGGTGTA 
CCAAGAACTA TTACCCCAAG GAGGATAACT CCAACTGCAG TATCTTCCAG TAGTGGAGGC 
AGCCGGAGAG TCACCACACT GCTGTCCCCA GCCCCTTCTT GCCAGGGTCA AAATCAGGAA 
ACCAATGCCT TCAATCCCCC TGTAAACCAG ACTGCAGCAA CCAAGAGAGA CACTCATGAG 
TTGAAAAAAT CTTTGGAAAA CGAGAGGGAG TTGCTTAAGC AAGAAAAAAT GAAACAGAAA 
AATCAGCCAG AATGTAGTCC ATTCTCTCAA AATGCATTAG GACAGTCACC AAGAGGAATG 
TCTGTATTGC AATCTCCAAA GGGTATGACT CTAGCTCATA CCCCGACTAA GCTTCTGCGC 
AGTAATTCAG TTTCAAGCAT AACTGAGGCA ATACTACCTG ATGTGGAAAA AGTAACAGGG 
CATGACAAAC TGGATCTCAT AGCAGAGCTC TATGCATCAT TTCTACAAGA GAGCCTTGTT 
CCCAATTTGT CTGTGGAGTT GTACTTCCTG ATTCAGCTGT TGACTGCTAA AGGTAGCAAG 
CTTGATGAGG ATGAGGGAAT ACATTCACTG GAAGAGGACA TTGAAGACAT CAATTATCTC 
TCAACAATCC ACAACTGTGT TTACTTTGCT GCATCAGTGC TCAGCAGGCT GGTTGTAGTG 
TTATCTCTGC TGGATAAGAG CACCTTACGC CTCCTGGCGG ACAACCAAGT GGTCTCTCAG 
TTCAATCCCG AGCTGAAGAC CAGGCTTGAA CAGGTCACCA ATCATTGCCC AACGGAGTCT 
GGTCTTCAGG CTGCCATTAC ATCTCCCATG CAGGTTGTCC CGTTCTTGGC AGATACCGAC 
AACAGGCAGA ACTTTCCAAG TGACACTTCA TTCCACAGTT TCAAGAAACA GAGAGATGGT 
TTCTATAGTC TGATACGAGA CTGGGAAGAT AATCGCAGTA AACCTGGCTG GAAGATGCAG 
GAGAAACTAA CCCACAGGAT CAGGGATTTA ATGAAGCACA TGGATGTGAC AAACTACACC 
CACTTCTCAA GACTGTTCCA GTCACAACTG ATCAAGATGT GCCAAGGTGA TAGCTCGGTG 
GTTGATAGTG GAGATTCTCA GGAATTCAGC TTCATCCAGC AGCTGAGACG GACGAATCCA 
GGAAAGTTTA AAAGATTGGA GGAGCGTTTT TCAACTCCAG CTTCATCCGG TGGTCCATGC 
CCTCCTCCTT CGTTCCATGG ATGTCAGGAA TTCTTTAGAG ATTTCATCCT AGCTACAAAC 
AGTTACATAT TCAACCAGCA TTTGACGGAC AGCTTTGCTA CCAAGCTCAT TGAGCTTAAC 
GATTTACAGT TGCCAATCAC TGATCAGGTT GATATGACTT CTGTTGTTGA TGCGGAGGTA 
AGGGAAAGCT TCAGTTCATG TCTCCTAGCC CTGAGGGTCT TGTCCAAGTT CCTGGGATTT 
CTAGTCTTTC TACCTTACCA GGGTGACAAT AATCTTCCTG ATATCATGAA GGATAATGTT 
ATCATGATGA GGAGTAGGGC ATCTCTTCCC GTGGACATTC TTGGATGTTT GTCTGCATCA 
GAAACCAACC AGCGTCTGAT CCTGACCTTG CCCTGGGTCG TAGAGTATCT TAGTATGATA 
GATGGGCTGG CCTCTCAGAT ACCAGTTTAT CATCAAACAC TCAGTTTATT GGGGGATATT 
CACAGGAGGG TGTCTGTGCA ATGGTCTGAG AATCCTAGCA ACCATAATTC ACTTCTTATC 
TTGCTTCTCT TGGGATGGCT GTTCGAGAAC CCAGCTCTAC CAAGCAATCT ACATCTGACG 
AGTGGTATGA GCGTTGAGAG GGGTAGCCAT AGTAATAAAC CAGGATCTGA CCAATCTTTA 
GACCCCCTTC CTCTGGTGGA TAGGCTGGTT CTCTATACAT GCTGCCCTTT CCTAGGTGAT 
TTAAGAGGAC TGTTATCCGA GGCTAGTATG GGGGTGAACA ACAGGAATGC TCCGGTCAAG 
AAGATTACTC CTGTAGCAGT GGATGCACCT CAGACTGCCA TTATCACTCA GCACCAAATA 
CAGGTGGAAC TTGAGAAGAA CTTCTTCCAC ATCCACTCCG CTTCCCTCAA GAAGAGCGTG 
GAGTTTGTGA GCGGCAGGGT CGCCTCCAAC TGCATCAAGT CCATCGATGG CTCACTAGTC 
TCTGCAGCCA GGCTCCAGAT TCCAAAGCTA CTCACTGAAG TGCTGGTCTC GTCCAGCAGC 
AGCAAGAAAG AGGCTGGGGC TCTCACAGCC AAGGCTAAGG GAGTTGGAGA GAAGCTGAGC 
CTGGAGACGA CAGAGCTTGC CAGGAAAGAA GCCAAGGAGT TTATCAAGAA GAAGTGTAGG 
TCAGCTCTTG ATGTGTTATT ACCGTGTGAG ACATCCGAGG CAGTCCTGGA CACTGCTTGT 
GCTATCACCA CTAGACTTGC CTTAGAACGA ACAGAGAAAT GGATCACAAC ACAAATACCT 
AGTGCATTAC AACAGGAGGT GACATTGCAG CTACCCAAGT GCCTGAGAGC TCAGAGCACT 
CAACAGATGA AGAAAGAATC AACCAGTAGG AAAATACCAG ACAAGCCAGC CATGCACGAC 
AGACAGGGGG CTTGTCCTTC AACCGTCATT CAAGAACTCA AGGACGGTCT TCATAGAATT 
TTAATTGGAG ACACCGATGG AAACACAGCA GAGGGCATCT GTTCATCCAT CAATAACGTT 
CATATGATGA TCAACAACAG ACAGGACCTA ACACCATTGG CTTACCAGTC CATCCACCAA 
CTCACCATAG AGCTGTGTGT AAGCCTGGCT ATGAGACAAC CAACTCTGCT TCAGACCTCT 
GTCTGTACCT CACCTACAAT CTCCAAAGGG CAAAGGTCCA TCGTTCTACC ACAGGACAGT 
AATACACACG GCAAGAACTG TAGCAGCAGC TTACACAATA CAGAGAATGG CAAACACCAA 
GACAAGAACT TGATATGTTC AACACAGGGA GTAGTTGAAG ATGGAGAGGA GCAGTGCAAT 
AACCAAGAGA GTGGTTTACA CTCACATCTT GGGAGATATG GACAAGAACT TCAGTGCAAT 
ACAAAGGGAG GACAGGGTAC AGAGGAAGGA GCATTCACCA CTCCTGTTCA TAAACATGCA 
TGGAATGGTG ACCAAGGTGA AAGAGATGTA ACTAATGCAG AACAGACAGA AGGAGATGTG 
ACTTTACACC GGTCTAATAC GGATACACTC TTAGAGAGAC TGATGGGTTT GTGGAGGAAT 
GTTTTTTCTT TGAGTATTCC ACCTGGAGCT CTGTTGAGTG CAAGGACAGC ATGGATGATA 
TCACACAGTC ATAACCCACA GGTTTCCTGG CAGTGTCTCA TCCATATAGT GAAGTCCCTT 
CTTGCATGCA ATCTGGTCGG CATTGGGGCT GTCCAATCAG CTTTGAGCAA GATGATGATG 
TCATCATACA TTCCACCAAT TACAGAGGAT GTAGCAAGAA GCCTATTACC TCTGGTCACA 
GATAATGAAA TAGGTATAGA TCAGAATGCA TTGGACTTGC TGCATGCAGT TTGCTCCGAA 
GCAAGAGAGA ATGAAGATGC CTATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011663040.1
CDS	322..4671
Translation	MAVILEHILSTKLSIQEVLEWSVEGKVEVSNSGFADLLRDTKLQTEFLPFFINSLRDQTAHLVCRGSSAVSSPARTPSSLQKLKRANQTRPQRSGGSKVNRTQLFAADNSKSSWSDKDFTPVSRLTAVEQSPHTPSSSKTNGRHGRNVSWSGGGGDAGSSSRRKTPGSGSRKDESITLAQFLVTPESQSRGKGRKSPFEDSNCDSSSSHKSSSSRRRSGGKGKQTGQNESGGSKGQHKLKYIDKPVFDLENAQEFPPMGVPRTITPRRITPTAVSSSSGGSRRVTTLLSPAPSCQGQNQETNAFNPPVNQTAATKRDTHELKKSLENERELLKQEKMKQKNQPECSPFSQNALGQSPRGMSVLQSPKGMTLAHTPTKLLRSNSVSSITEAILPDVEKVTGHDKLDLIAELYASFLQESLVPNLSVELYFLIQLLTAKGSKLDEDEGIHSLEEDIEDINYLSTIHNCVYFAASVLSRLVVVLSLLDKSTLRLLADNQVVSQFNPELKTRLEQVTNHCPTESGLQAAITSPMQVVPFLADTDNRQNFPSDTSFHSFKKQRDGFYSLIRDWEDNRSKPGWKMQEKLTHRIRDLMKHMDVTNYTHFSRLFQSQLIKMCQGDSSVVDSGDSQEFSFIQQLRRTNPGKFKRLEERFSTPASSGGPCPPPSFHGCQEFFRDFILATNSYIFNQHLTDSFATKLIELNDLQLPITDQVDMTSVVDAEVRESFSSCLLALRVLSKFLGFLVFLPYQGDNNLPDIMKDNVIMMRSRASLPVDILGCLSASETNQRLILTLPWVVEYLSMIDGLASQIPVYHQTLSLLGDIHRRVSVQWSENPSNHNSLLILLLLGWLFENPALPSNLHLTSGMSVERGSHSNKPGSDQSLDPLPLVDRLVLYTCCPFLGDLRGLLSEASMGVNNRNAPVKKITPVAVDAPQTAIITQHQIQVELEKNFFHIHSASLKKSVEFVSGRVASNCIKSIDGSLVSAARLQIPKLLTEVLVSSSSSKKEAGALTAKAKGVGEKLSLETTELARKEAKEFIKKKCRSALDVLLPCETSEAVLDTACAITTRLALERTEKWITTQIPSALQQEVTLQLPKCLRAQSTQQMKKESTSRKIPDKPAMHDRQGACPSTVIQELKDGLHRILIGDTDGNTAEGICSSINNVHMMINNRQDLTPLAYQSIHQLTIELCVSLAMRQPTLLQTSVCTSPTISKGQRSIVLPQDSNTHGKNCSSSLHNTENGKHQDKNLICSTQGVVEDGEEQCNNQESGLHSHLGRYGQELQCNTKGGQGTEEGAFTTPVHKHAWNGDQGERDVTNAEQTEGDVTLHRSNTDTLLERLMGLWRNVFSLSIPPGALLSARTAWMISHSHNPQVSWQCLIHIVKSLLACNLVGIGAVQSALSKMMMSSYIPPITEDVARSLLPLVTDNEIGIDQNALDLLHAVCSEARENEDAYS

Target ORF information:

RefSeq Version	XM_011664738.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011664738.1

ORF Insert Sequence:

ATGGCGGTGA TATTGGAACA CATTTTGTCA ACAAAATTGT CAATACAAGA AGTGTTGGAG 
TGGTCAGTCG AAGGGAAGGT TGAAGTGAGT AACTCTGGAT TTGCAGACTT GTTGAGGGAT 
ACCAAACTGC AAACAGAATT TCTACCATTC TTCATCAATA GCTTGCGGGA TCAGACAGCC 
CACTTAGTAT GCAGGGGATC ATCTGCTGTG TCTTCCCCAG CGAGGACGCC AAGCAGCTTA 
CAAAAACTAA AAAGAGCCAA TCAGACACGC CCTCAGCGCA GTGGAGGTTC AAAGGTCAAT 
CGTACACAGC TGTTTGCTGC TGATAACAGC AAGTCCAGCT GGTCAGACAA AGACTTTACA 
CCCGTTTCAA GACTAACAGC AGTAGAGCAG TCGCCGCACA CTCCATCGTC ATCTAAGACA 
AATGGAAGAC ACGGAAGAAA TGTGTCTTGG AGTGGTGGTG GCGGTGATGC TGGCTCCAGC 
TCCAGGAGGA AGACTCCCGG ATCAGGATCT AGGAAGGATG AATCCATAAC GCTGGCTCAG 
TTCTTGGTGA CTCCCGAAAG CCAGTCAAGA GGAAAGGGCA GAAAGAGTCC TTTTGAAGAC 
TCTAACTGTG ATAGCTCATC GTCACATAAA AGCTCATCCA GTAGACGTCG CTCAGGAGGT 
AAAGGAAAGC AAACGGGACA GAATGAGAGC GGTGGCTCGA AAGGACAGCA TAAATTGAAA 
TACATTGACA AACCTGTGTT TGACTTGGAA AATGCCCAAG AATTCCCTCC AATGGGTGTA 
CCAAGAACTA TTACCCCAAG GAGGATAACT CCAACTGCAG TATCTTCCAG TAGTGGAGGC 
AGCCGGAGAG TCACCACACT GCTGTCCCCA GCCCCTTCTT GCCAGGGTCA AAATCAGGAA 
ACCAATGCCT TCAATCCCCC TGTAAACCAG ACTGCAGCAA CCAAGAGAGA CACTCATGAG 
TTGAAAAAAT CTTTGGAAAA CGAGAGGGAG TTGCTTAAGC AAGAAAAAAT GAAACAGAAA 
AATCAGCCAG AATGTAGTCC ATTCTCTCAA AATGCATTAG GACAGTCACC AAGAGGAATG 
TCTGTATTGC AATCTCCAAA GGGTATGACT CTAGCTCATA CCCCGACTAA GCTTCTGCGC 
AGTAATTCAG TTTCAAGCAT AACTGAGGCA ATACTACCTG ATGTGGAAAA AGTAACAGGG 
CATGACAAAC TGGATCTCAT AGCAGAGCTC TATGCATCAT TTCTACAAGA GAGCCTTGTT 
CCCAATTTGT CTGTGGAGTT GTACTTCCTG ATTCAGCTGT TGACTGCTAA AGGTAGCAAG 
CTTGATGAGG ATGAGGGAAT ACATTCACTG GAAGAGGACA TTGAAGACAT CAATTATCTC 
TCAACAATCC ACAACTGTGT TTACTTTGCT GCATCAGTGC TCAGCAGGCT GGTTGTAGTG 
TTATCTCTGC TGGATAAGAG CACCTTACGC CTCCTGGCGG ACAACCAAGT GGTCTCTCAG 
TTCAATCCCG AGCTGAAGAC CAGGCTTGAA CAGGTCACCA ATCATTGCCC AACGGAGTCT 
GGTCTTCAGG CTGCCATTAC ATCTCCCATG CAGGTTGTCC CGTTCTTGGC AGATACCGAC 
AACAGGCAGA ACTTTCCAAG TGACACTTCA TTCCACAGTT TCAAGAAACA GAGAGATGGT 
TTCTATAGTC TGATACGAGA CTGGGAAGAT AATCGCAGTA AACCTGGCTG GAAGATGCAG 
GAGAAACTAA CCCACAGGAT CAGGGATTTA ATGAAGCACA TGGATGTGAC AAACTACACC 
CACTTCTCAA GACTGTTCCA GTCACAACTG ATCAAGATGT GCCAAGGTGA TAGCTCGGTG 
GTTGATAGTG GAGATTCTCA GGAATTCAGC TTCATCCAGC AGCTGAGACG GACGAATCCA 
GGAAAGTTTA AAAGATTGGA GGAGCGTTTT TCAACTCCAG CTTCATCCGG TGGTCCATGC 
CCTCCTCCTT CGTTCCATGG ATGTCAGGAA TTCTTTAGAG ATTTCATCCT AGCTACAAAC 
AGTTACATAT TCAACCAGCA TTTGACGGAC AGCTTTGCTA CCAAGCTCAT TGAGCTTAAC 
GATTTACAGT TGCCAATCAC TGATCAGGTT GATATGACTT CTGTTGTTGA TGCGGAGGTA 
AGGGAAAGCT TCAGTTCATG TCTCCTAGCC CTGAGGGTCT TGTCCAAGTT CCTGGGATTT 
CTAGTCTTTC TACCTTACCA GGGTGACAAT AATCTTCCTG ATATCATGAA GGATAATGTT 
ATCATGATGA GGAGTAGGGC ATCTCTTCCC GTGGACATTC TTGGATGTTT GTCTGCATCA 
GAAACCAACC AGCGTCTGAT CCTGACCTTG CCCTGGGTCG TAGAGTATCT TAGTATGATA 
GATGGGCTGG CCTCTCAGAT ACCAGTTTAT CATCAAACAC TCAGTTTATT GGGGGATATT 
CACAGGAGGG TGTCTGTGCA ATGGTCTGAG AATCCTAGCA ACCATAATTC ACTTCTTATC 
TTGCTTCTCT TGGGATGGCT GTTCGAGAAC CCAGCTCTAC CAAGCAATCT ACATCTGACG 
AGTGGTATGA GCGTTGAGAG GGGTAGCCAT AGTAATAAAC CAGGATCTGA CCAATCTTTA 
GACCCCCTTC CTCTGGTGGA TAGGCTGGTT CTCTATACAT GCTGCCCTTT CCTAGGTGAT 
TTAAGAGGAC TGTTATCCGA GGCTAGTATG GGGGTGAACA ACAGGAATGC TCCGGTCAAG 
AAGATTACTC CTGTAGCAGT GGATGCACCT CAGACTGCCA TTATCACTCA GCACCAAATA 
CAGGTGGAAC TTGAGAAGAA CTTCTTCCAC ATCCACTCCG CTTCCCTCAA GAAGAGCGTG 
GAGTTTGTGA GCGGCAGGGT CGCCTCCAAC TGCATCAAGT CCATCGATGG CTCACTAGTC 
TCTGCAGCCA GGCTCCAGAT TCCAAAGCTA CTCACTGAAG TGCTGGTCTC GTCCAGCAGC 
AGCAAGAAAG AGGCTGGGGC TCTCACAGCC AAGGCTAAGG GAGTTGGAGA GAAGCTGAGC 
CTGGAGACGA CAGAGCTTGC CAGGAAAGAA GCCAAGGAGT TTATCAAGAA GAAGTGTAGG 
TCAGCTCTTG ATGTGTTATT ACCGTGTGAG ACATCCGAGG CAGTCCTGGA CACTGCTTGT 
GCTATCACCA CTAGACTTGC CTTAGAACGA ACAGAGAAAT GGATCACAAC ACAAATACCT 
AGTGCATTAC AACAGGAGGT GACATTGCAG CTACCCAAGT GCCTGAGAGC TCAGAGCACT 
CAACAGATGA AGAAAGAATC AACCAGTAGG AAAATACCAG ACAAGCCAGC CATGCACGAC 
AGACAGGGGG CTTGTCCTTC AACCGTCATT CAAGAACTCA AGGACGGTCT TCATAGAATT 
TTAATTGGAG ACACCGATGG AAACACAGCA GAGGGCATCT GTTCATCCAT CAATAACGTT 
CATATGATGA TCAACAACAG ACAGGACCTA ACACCATTGG CTTACCAGTC CATCCACCAA 
CTCACCATAG AGCTGTGTGT AAGCCTGGCT ATGAGACAAC CAACTCTGCT TCAGACCTCT 
GTCTGTACCT CACCTACAAT CTCCAAAGGG CAAAGGTCCA TCGTTCTACC ACAGGACAGT 
AATACACACG GCAAGAACTG TAGCAGCAGC TTACACAATA CAGAGAATGG CAAACACCAA 
GACAAGAACT TGATATGTTC AACACAGGGA GTAGTTGAAG ATGGAGAGGA GCAGTGCAAT 
AACCAAGAGA GTGGTTTACA CTCACATCTT GGGAGATATG GACAAGAACT TCAGTGCAAT 
ACAAAGGGAG GACAGGGTAC AGAGGAAGGA GCATTCACCA CTCCTGTTCA TAAACATGCA 
TGGAATGGTG ACCAAGGTGA AAGAGATGTA ACTAATGCAG AACAGACAGA AGGAGATGTG 
ACTTTACACC GGTCTAATAC GGATACACTC TTAGAGAGAC TGATGGGTTT GTGGAGGAAT 
GTTTTTTCTT TGAGTATTCC ACCTGGAGCT CTGTTGAGTG CAAGGACAGC ATGGATGATA 
TCACACAGTC ATAACCCACA GGTTTCCTGG CAGTGTCTCA TCCATATAGT GAAGTCCCTT 
CTTGCATGCA ATCTGGTCGG CATTGGGGCT GTCCAATCAG CTTTGAGCAA GATGATGATG 
TCATCATACA TTCCACCAAT TACAGAGGAT GTAGCAAGAA GCCTATTACC TCTGGTCACA 
GATAATGAAA TAGGTATAGA TCAGAATGCA TTGGACTTGC TGCATGCAGT TTGCTCCGAA 
GCAAGAGAGA ATGAAGATGC CTATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC579713 ( XM_011664739.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011663041.1 Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X3, mRNA.

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CloneID	OSd22843
Clone ID Related Accession (Same CDS sequence)	XM_011664739.1
Accession Version	XM_011664739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	codanin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011998944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGA TATTGGAACA CATTTTGTCA ACAAAATTGT CAATACAAGA AGTGTTGGAG 
TGGTCAGTCG AAGGGAAGGT TGAAAACTCT GGATTTGCAG ACTTGTTGAG GGATACCAAA 
CTGCAAACAG AATTTCTACC ATTCTTCATC AATAGCTTGC GGGATCAGAC AGCCCACTTA 
GTATGCAGGG GATCATCTGC TGTGTCTTCC CCAGCGAGGA CGCCAAGCAG CTTACAAAAA 
CTAAAAAGAG CCAATCAGAC ACGCCCTCAG CGCAGTGGAG GTTCAAAGGT CAATCGTACA 
CAGCTGTTTG CTGCTGATAA CAGCAAGTCC AGCTGGTCAG ACAAAGACTT TACACCCGTT 
TCAAGACTAA CAGCAGTAGA GCAGTCGCCG CACACTCCAT CGTCATCTAA GACAAATGGA 
AGACACGGAA GAAATGTGTC TTGGAGTGGT GGTGGCGGTG ATGCTGGCTC CAGCTCCAGG 
AGGAAGACTC CCGGATCAGG ATCTAGGAAG GATGAATCCA TAACGCTGGC TCAGTTCTTG 
GTGACTCCCG AAAGCCAGTC AAGAGGAAAG GGCAGAAAGA GTCCTTTTGA AGACTCTAAC 
TGTGATAGCT CATCGTCACA TAAAAGCTCA TCCAGTAGAC GTCGCTCAGG AGGTAAAGGA 
AAGCAAACGG GACAGAATGA GAGCGGTGGC TCGAAAGGAC AGCATAAATT GAAATACATT 
GACAAACCTG TGTTTGACTT GGAAAATGCC CAAGAATTCC CTCCAATGGG TGTACCAAGA 
ACTATTACCC CAAGGAGGAT AACTCCAACT GCAGTATCTT CCAGTAGTGG AGGCAGCCGG 
AGAGTCACCA CACTGCTGTC CCCAGCCCCT TCTTGCCAGG GTCAAAATCA GGAAACCAAT 
GCCTTCAATC CCCCTGTAAA CCAGACTGCA GCAACCAAGA GAGACACTCA TGAGTTGAAA 
AAATCTTTGG AAAACGAGAG GGAGTTGCTT AAGCAAGAAA AAATGAAACA GAAAAATCAG 
CCAGAATGTA GTCCATTCTC TCAAAATGCA TTAGGACAGT CACCAAGAGG AATGTCTGTA 
TTGCAATCTC CAAAGGGTAT GACTCTAGCT CATACCCCGA CTAAGCTTCT GCGCAGTAAT 
TCAGTTTCAA GCATAACTGA GGCAATACTA CCTGATGTGG AAAAAGTAAC AGGGCATGAC 
AAACTGGATC TCATAGCAGA GCTCTATGCA TCATTTCTAC AAGAGAGCCT TGTTCCCAAT 
TTGTCTGTGG AGTTGTACTT CCTGATTCAG CTGTTGACTG CTAAAGGTAG CAAGCTTGAT 
GAGGATGAGG GAATACATTC ACTGGAAGAG GACATTGAAG ACATCAATTA TCTCTCAACA 
ATCCACAACT GTGTTTACTT TGCTGCATCA GTGCTCAGCA GGCTGGTTGT AGTGTTATCT 
CTGCTGGATA AGAGCACCTT ACGCCTCCTG GCGGACAACC AAGTGGTCTC TCAGTTCAAT 
CCCGAGCTGA AGACCAGGCT TGAACAGGTC ACCAATCATT GCCCAACGGA GTCTGGTCTT 
CAGGCTGCCA TTACATCTCC CATGCAGGTT GTCCCGTTCT TGGCAGATAC CGACAACAGG 
CAGAACTTTC CAAGTGACAC TTCATTCCAC AGTTTCAAGA AACAGAGAGA TGGTTTCTAT 
AGTCTGATAC GAGACTGGGA AGATAATCGC AGTAAACCTG GCTGGAAGAT GCAGGAGAAA 
CTAACCCACA GGATCAGGGA TTTAATGAAG CACATGGATG TGACAAACTA CACCCACTTC 
TCAAGACTGT TCCAGTCACA ACTGATCAAG ATGTGCCAAG GTGATAGCTC AGTGGTTGAT 
AGTGGAGATT CTCAGGAATT TAGCTTCATC CAGCAGCTGA GACGGACGAA TCCAGGAAAG 
TTTAAAAGAT TGGAGGAGCG TTTTTCAACT CCAGCTTCAT CCGGTGGTCC ATGCCCTCCT 
CCTTCGTTCC ATGGATGTCA GGAATTCTTT AGAGATTTCA TCCTAGCTAC AAACAGTTAC 
ATATTCAACC AGCATTTGAC GGACAGCTTT GCTACCAAGC TCATTGAGCT TAACGATTTA 
CAGTTGCCAA TCACTGATCA GGTTGATATG ACTTCTGTTG TTGATGCGGA GGTAAGGGAA 
AGCTTCAGTT CATGTCTCCT AGCCCTGAGG GTCTTGTCCA AGTTCCTGGG ATTTCTAGTC 
TTTCTACCTT ACCAGGGTGA CAATAATCTT CCTGATATCA TGAAGGATAA TGTTATCATG 
ATGAGGAGTA GGGCATCTCT TCCCGTGGAC ATTCTTGGAT GTTTGTCTGC ATCAGAAACC 
AACCAGCGTC TGATCCTGAC CTTGCCCTGG GTCGTAGAGT ATCTTAGTAT GATAGATGGG 
CTGGCCTCTC AGATACCAGT TTATCATCAA ACACTCAGTT TATTGGGGGA TATTCACAGG 
AGGGTGTCTG TGCAATGGTC TGAGAATCCT AGCAACCATA ATTCACTTCT TATCTTGCTT 
CTCTTGGGAT GGCTGTTCGA GAACCCAGCT CTACCAAGCA ATCTACATCT GACGAGTGGT 
ATGAGCGTTG AGAGGGGTAG CCATAGTAAT AAACCAGGAT CTGACCAATC TTTAGACCCC 
CTTCCTCTGG TGGATAGGCT GGTTCTCTAT ACATGCTGCC CTTTCCTAGG TGATTTAAGA 
GGACTGTTAT CCGAGGCTAG TATGGGGGTG AACAACAGGA ATGCTCCGGT CAAGAAGATT 
ACTCCTGTAG CAGTGGATGC ACCTCAGACT GCCATTATCA CTCAGCACCA AATACAGGTG 
GAACTTGAGA AGAACTTCTT CCACATCCAC TCCGCTTCCC TCAAGAAGAG CGTGGAGTTT 
GTGAGCGGCA GGGTCGCCTC CAACTGCATC AAGTCCATCG ATGGCTCACT AGTCTCTGCA 
GCCAGGCTCC AGATTCCAAA GCTACTCACT GAAGTGCTGG TCTCGTCCAG CAGCAGCAAG 
AAAGAGGCTG GGGCTCTCAC AGCCAAGGCT AAGGGAGTTG GAGAGAAGCT GAGCCTGGAG 
ACGACAGAGC TTGCCAGGAA AGAAGCCAAG GAGTTTATCA AGAAGAAGTG TAGGTCAGCT 
CTTGATGTGT TATTACCGTG TGAGACATCC GAGGCAGTCC TGGACACTGC TTGTGCTATC 
ACCACTAGAC TTGCCTTAGA ACGAACAGAG AAATGGATCA CAACACAAAT ACCTAGTGCA 
TTACAACAGG AGGTGACATT GCAGCTACCC AAGTGCCTGA GAGCTCAGAG CACTCAACAG 
ATGAAGAAAG AATCAACCAG TAGGAAAATA CCAGACAAGC CAGCCATGCA CGACAGACAG 
GGGGCTTGTC CTTCAACCGT CATTCAAGAA CTCAAGGACG GTCTTCATAG AATTTTAATT 
GGAGACACCG ATGGAAACAC AGCAGAGGGC ATCTGTTCAT CCATCAATAA CGTTCATATG 
ATGATCAACA ACAGACAGGA CCTAACACCA TTGGCTTACC AGTCCATCCA CCAACTCACC 
ATAGAGCTGT GTGTAAGCCT GGCTATGAGA CAACCAACTC TGCTTCAGAC CTCTGTCTGT 
ACCTCACCTA CAATCTCCAA AGGGCAAAGG TCCATCGTTC TACCACAGGA CAGTAATACA 
CACGGCAAGA ACTGTAGCAG CAGCTTACAC AATACAGAGA ATGGCAAACA CCAAGACAAG 
AACTTGATAT GTTCAACACA GGGAGTAGTT GAAGATGGAG AGGAGCAGTG CAATAACCAA 
GAGAGTGGTT TACACTCACA TCTTGGGAGA TATGGACAAG AACTTCAGTG CAATACAAAG 
GGAGGACAGG GTACAGAGGA AGGAGCATTC ACCACTCCTG TTCATAAACA TGCATGGAAT 
GGTGACCAAG GTGAAAGAGA TGTAACTAAT GCAGAACAGA CAGAAGGAGA TGTGACTTTA 
CACCGGTCTA ATACGGATAC ACTCTTAGAG AGACTGATGG GTTTGTGGAG GAATGTTTTT 
TCTTTGAGTA TTCCACCTGG AGCTCTGTTG AGTGCAAGGA CAGCATGGAT GATATCACAC 
AGTCATAACC CACAGGTTTC CTGGCAGTGT CTCATCCATA TAGTGAAGTC CCTTCTTGCA 
TGCAATCTGG TCGGCATTGG GGCTGTCCAA TCAGCTTTGA GCAAGATGAT GATGTCATCA 
TACATTCCAC CAATTACAGA GGATGTAGCA AGAAGCCTAT TACCTCTGGT CACAGATAAT 
GAAATAGGTA TAGATCAGAA TGCATTGGAC TTGCTGCATG CAGTTTGCTC CGAAGCAAGA 
GAGAATGAAG ATGCCTATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011663041.1
CDS	322..4665
Translation	MAVILEHILSTKLSIQEVLEWSVEGKVENSGFADLLRDTKLQTEFLPFFINSLRDQTAHLVCRGSSAVSSPARTPSSLQKLKRANQTRPQRSGGSKVNRTQLFAADNSKSSWSDKDFTPVSRLTAVEQSPHTPSSSKTNGRHGRNVSWSGGGGDAGSSSRRKTPGSGSRKDESITLAQFLVTPESQSRGKGRKSPFEDSNCDSSSSHKSSSSRRRSGGKGKQTGQNESGGSKGQHKLKYIDKPVFDLENAQEFPPMGVPRTITPRRITPTAVSSSSGGSRRVTTLLSPAPSCQGQNQETNAFNPPVNQTAATKRDTHELKKSLENERELLKQEKMKQKNQPECSPFSQNALGQSPRGMSVLQSPKGMTLAHTPTKLLRSNSVSSITEAILPDVEKVTGHDKLDLIAELYASFLQESLVPNLSVELYFLIQLLTAKGSKLDEDEGIHSLEEDIEDINYLSTIHNCVYFAASVLSRLVVVLSLLDKSTLRLLADNQVVSQFNPELKTRLEQVTNHCPTESGLQAAITSPMQVVPFLADTDNRQNFPSDTSFHSFKKQRDGFYSLIRDWEDNRSKPGWKMQEKLTHRIRDLMKHMDVTNYTHFSRLFQSQLIKMCQGDSSVVDSGDSQEFSFIQQLRRTNPGKFKRLEERFSTPASSGGPCPPPSFHGCQEFFRDFILATNSYIFNQHLTDSFATKLIELNDLQLPITDQVDMTSVVDAEVRESFSSCLLALRVLSKFLGFLVFLPYQGDNNLPDIMKDNVIMMRSRASLPVDILGCLSASETNQRLILTLPWVVEYLSMIDGLASQIPVYHQTLSLLGDIHRRVSVQWSENPSNHNSLLILLLLGWLFENPALPSNLHLTSGMSVERGSHSNKPGSDQSLDPLPLVDRLVLYTCCPFLGDLRGLLSEASMGVNNRNAPVKKITPVAVDAPQTAIITQHQIQVELEKNFFHIHSASLKKSVEFVSGRVASNCIKSIDGSLVSAARLQIPKLLTEVLVSSSSSKKEAGALTAKAKGVGEKLSLETTELARKEAKEFIKKKCRSALDVLLPCETSEAVLDTACAITTRLALERTEKWITTQIPSALQQEVTLQLPKCLRAQSTQQMKKESTSRKIPDKPAMHDRQGACPSTVIQELKDGLHRILIGDTDGNTAEGICSSINNVHMMINNRQDLTPLAYQSIHQLTIELCVSLAMRQPTLLQTSVCTSPTISKGQRSIVLPQDSNTHGKNCSSSLHNTENGKHQDKNLICSTQGVVEDGEEQCNNQESGLHSHLGRYGQELQCNTKGGQGTEEGAFTTPVHKHAWNGDQGERDVTNAEQTEGDVTLHRSNTDTLLERLMGLWRNVFSLSIPPGALLSARTAWMISHSHNPQVSWQCLIHIVKSLLACNLVGIGAVQSALSKMMMSSYIPPITEDVARSLLPLVTDNEIGIDQNALDLLHAVCSEARENEDAYS

Target ORF information:

RefSeq Version	XM_011664739.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011664739.1

ORF Insert Sequence:

ATGGCGGTGA TATTGGAACA CATTTTGTCA ACAAAATTGT CAATACAAGA AGTGTTGGAG 
TGGTCAGTCG AAGGGAAGGT TGAAAACTCT GGATTTGCAG ACTTGTTGAG GGATACCAAA 
CTGCAAACAG AATTTCTACC ATTCTTCATC AATAGCTTGC GGGATCAGAC AGCCCACTTA 
GTATGCAGGG GATCATCTGC TGTGTCTTCC CCAGCGAGGA CGCCAAGCAG CTTACAAAAA 
CTAAAAAGAG CCAATCAGAC ACGCCCTCAG CGCAGTGGAG GTTCAAAGGT CAATCGTACA 
CAGCTGTTTG CTGCTGATAA CAGCAAGTCC AGCTGGTCAG ACAAAGACTT TACACCCGTT 
TCAAGACTAA CAGCAGTAGA GCAGTCGCCG CACACTCCAT CGTCATCTAA GACAAATGGA 
AGACACGGAA GAAATGTGTC TTGGAGTGGT GGTGGCGGTG ATGCTGGCTC CAGCTCCAGG 
AGGAAGACTC CCGGATCAGG ATCTAGGAAG GATGAATCCA TAACGCTGGC TCAGTTCTTG 
GTGACTCCCG AAAGCCAGTC AAGAGGAAAG GGCAGAAAGA GTCCTTTTGA AGACTCTAAC 
TGTGATAGCT CATCGTCACA TAAAAGCTCA TCCAGTAGAC GTCGCTCAGG AGGTAAAGGA 
AAGCAAACGG GACAGAATGA GAGCGGTGGC TCGAAAGGAC AGCATAAATT GAAATACATT 
GACAAACCTG TGTTTGACTT GGAAAATGCC CAAGAATTCC CTCCAATGGG TGTACCAAGA 
ACTATTACCC CAAGGAGGAT AACTCCAACT GCAGTATCTT CCAGTAGTGG AGGCAGCCGG 
AGAGTCACCA CACTGCTGTC CCCAGCCCCT TCTTGCCAGG GTCAAAATCA GGAAACCAAT 
GCCTTCAATC CCCCTGTAAA CCAGACTGCA GCAACCAAGA GAGACACTCA TGAGTTGAAA 
AAATCTTTGG AAAACGAGAG GGAGTTGCTT AAGCAAGAAA AAATGAAACA GAAAAATCAG 
CCAGAATGTA GTCCATTCTC TCAAAATGCA TTAGGACAGT CACCAAGAGG AATGTCTGTA 
TTGCAATCTC CAAAGGGTAT GACTCTAGCT CATACCCCGA CTAAGCTTCT GCGCAGTAAT 
TCAGTTTCAA GCATAACTGA GGCAATACTA CCTGATGTGG AAAAAGTAAC AGGGCATGAC 
AAACTGGATC TCATAGCAGA GCTCTATGCA TCATTTCTAC AAGAGAGCCT TGTTCCCAAT 
TTGTCTGTGG AGTTGTACTT CCTGATTCAG CTGTTGACTG CTAAAGGTAG CAAGCTTGAT 
GAGGATGAGG GAATACATTC ACTGGAAGAG GACATTGAAG ACATCAATTA TCTCTCAACA 
ATCCACAACT GTGTTTACTT TGCTGCATCA GTGCTCAGCA GGCTGGTTGT AGTGTTATCT 
CTGCTGGATA AGAGCACCTT ACGCCTCCTG GCGGACAACC AAGTGGTCTC TCAGTTCAAT 
CCCGAGCTGA AGACCAGGCT TGAACAGGTC ACCAATCATT GCCCAACGGA GTCTGGTCTT 
CAGGCTGCCA TTACATCTCC CATGCAGGTT GTCCCGTTCT TGGCAGATAC CGACAACAGG 
CAGAACTTTC CAAGTGACAC TTCATTCCAC AGTTTCAAGA AACAGAGAGA TGGTTTCTAT 
AGTCTGATAC GAGACTGGGA AGATAATCGC AGTAAACCTG GCTGGAAGAT GCAGGAGAAA 
CTAACCCACA GGATCAGGGA TTTAATGAAG CACATGGATG TGACAAACTA CACCCACTTC 
TCAAGACTGT TCCAGTCACA ACTGATCAAG ATGTGCCAAG GTGATAGCTC AGTGGTTGAT 
AGTGGAGATT CTCAGGAATT TAGCTTCATC CAGCAGCTGA GACGGACGAA TCCAGGAAAG 
TTTAAAAGAT TGGAGGAGCG TTTTTCAACT CCAGCTTCAT CCGGTGGTCC ATGCCCTCCT 
CCTTCGTTCC ATGGATGTCA GGAATTCTTT AGAGATTTCA TCCTAGCTAC AAACAGTTAC 
ATATTCAACC AGCATTTGAC GGACAGCTTT GCTACCAAGC TCATTGAGCT TAACGATTTA 
CAGTTGCCAA TCACTGATCA GGTTGATATG ACTTCTGTTG TTGATGCGGA GGTAAGGGAA 
AGCTTCAGTT CATGTCTCCT AGCCCTGAGG GTCTTGTCCA AGTTCCTGGG ATTTCTAGTC 
TTTCTACCTT ACCAGGGTGA CAATAATCTT CCTGATATCA TGAAGGATAA TGTTATCATG 
ATGAGGAGTA GGGCATCTCT TCCCGTGGAC ATTCTTGGAT GTTTGTCTGC ATCAGAAACC 
AACCAGCGTC TGATCCTGAC CTTGCCCTGG GTCGTAGAGT ATCTTAGTAT GATAGATGGG 
CTGGCCTCTC AGATACCAGT TTATCATCAA ACACTCAGTT TATTGGGGGA TATTCACAGG 
AGGGTGTCTG TGCAATGGTC TGAGAATCCT AGCAACCATA ATTCACTTCT TATCTTGCTT 
CTCTTGGGAT GGCTGTTCGA GAACCCAGCT CTACCAAGCA ATCTACATCT GACGAGTGGT 
ATGAGCGTTG AGAGGGGTAG CCATAGTAAT AAACCAGGAT CTGACCAATC TTTAGACCCC 
CTTCCTCTGG TGGATAGGCT GGTTCTCTAT ACATGCTGCC CTTTCCTAGG TGATTTAAGA 
GGACTGTTAT CCGAGGCTAG TATGGGGGTG AACAACAGGA ATGCTCCGGT CAAGAAGATT 
ACTCCTGTAG CAGTGGATGC ACCTCAGACT GCCATTATCA CTCAGCACCA AATACAGGTG 
GAACTTGAGA AGAACTTCTT CCACATCCAC TCCGCTTCCC TCAAGAAGAG CGTGGAGTTT 
GTGAGCGGCA GGGTCGCCTC CAACTGCATC AAGTCCATCG ATGGCTCACT AGTCTCTGCA 
GCCAGGCTCC AGATTCCAAA GCTACTCACT GAAGTGCTGG TCTCGTCCAG CAGCAGCAAG 
AAAGAGGCTG GGGCTCTCAC AGCCAAGGCT AAGGGAGTTG GAGAGAAGCT GAGCCTGGAG 
ACGACAGAGC TTGCCAGGAA AGAAGCCAAG GAGTTTATCA AGAAGAAGTG TAGGTCAGCT 
CTTGATGTGT TATTACCGTG TGAGACATCC GAGGCAGTCC TGGACACTGC TTGTGCTATC 
ACCACTAGAC TTGCCTTAGA ACGAACAGAG AAATGGATCA CAACACAAAT ACCTAGTGCA 
TTACAACAGG AGGTGACATT GCAGCTACCC AAGTGCCTGA GAGCTCAGAG CACTCAACAG 
ATGAAGAAAG AATCAACCAG TAGGAAAATA CCAGACAAGC CAGCCATGCA CGACAGACAG 
GGGGCTTGTC CTTCAACCGT CATTCAAGAA CTCAAGGACG GTCTTCATAG AATTTTAATT 
GGAGACACCG ATGGAAACAC AGCAGAGGGC ATCTGTTCAT CCATCAATAA CGTTCATATG 
ATGATCAACA ACAGACAGGA CCTAACACCA TTGGCTTACC AGTCCATCCA CCAACTCACC 
ATAGAGCTGT GTGTAAGCCT GGCTATGAGA CAACCAACTC TGCTTCAGAC CTCTGTCTGT 
ACCTCACCTA CAATCTCCAA AGGGCAAAGG TCCATCGTTC TACCACAGGA CAGTAATACA 
CACGGCAAGA ACTGTAGCAG CAGCTTACAC AATACAGAGA ATGGCAAACA CCAAGACAAG 
AACTTGATAT GTTCAACACA GGGAGTAGTT GAAGATGGAG AGGAGCAGTG CAATAACCAA 
GAGAGTGGTT TACACTCACA TCTTGGGAGA TATGGACAAG AACTTCAGTG CAATACAAAG 
GGAGGACAGG GTACAGAGGA AGGAGCATTC ACCACTCCTG TTCATAAACA TGCATGGAAT 
GGTGACCAAG GTGAAAGAGA TGTAACTAAT GCAGAACAGA CAGAAGGAGA TGTGACTTTA 
CACCGGTCTA ATACGGATAC ACTCTTAGAG AGACTGATGG GTTTGTGGAG GAATGTTTTT 
TCTTTGAGTA TTCCACCTGG AGCTCTGTTG AGTGCAAGGA CAGCATGGAT GATATCACAC 
AGTCATAACC CACAGGTTTC CTGGCAGTGT CTCATCCATA TAGTGAAGTC CCTTCTTGCA 
TGCAATCTGG TCGGCATTGG GGCTGTCCAA TCAGCTTTGA GCAAGATGAT GATGTCATCA 
TACATTCCAC CAATTACAGA GGATGTAGCA AGAAGCCTAT TACCTCTGGT CACAGATAAT 
GAAATAGGTA TAGATCAGAA TGCATTGGAC TTGCTGCATG CAGTTTGCTC CGAAGCAAGA 
GAGAATGAAG ATGCCTATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC579713 ( XM_011664737.2 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011663039.2 Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X1, mRNA.

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CloneID	OSd65759
Clone ID Related Accession (Same CDS sequence)	XM_011664737.2
Accession Version	XM_011664737.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	codanin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 27, 2019 this sequence version replaced XM_011664737.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGA TATTGGAACA CATTTTGTCA ACAAAATTGT CAATACAAGA AGTGTTGGAG 
TGGTCAGTCG AAGGGAAGGT TGAAGTGAGT AACTCTGGAT TTGCAGACTT GTTGAGGGAT 
ACCAAACTGC AAACAGAATT TCTACCATTC TTCATCAATA GCTTGCGGGA TCAGACAGCC 
CACTTAGTAT GCAGGGGATC ATCTGCTGTG TCTTCCCCAG CGAGGACGCC AAGCAGCTTA 
CAAAAACTAA AAAGAGCCAA TCAGACACGC CCTCAGCGCA GTGGAGGTTC AAAGGTCAAT 
CGTACACAGC TGTTTGCTGC TGATAACAGC AAGTCCAGCT GGTCAGACAA AGACTTTACA 
CCCGTTTCAA GACTAACAGC AGTAGAGCAG TCGCCGCACA CTCCATCGTC ATCTAAGACA 
AATGGAAGAC ACGGAAGAAA TGTGTCTTGG AGTGGTGGTG GCGGTGATGC TGGCTCCAGC 
TCCAGGAGGA AGACTCCCGG ATCAGGATCT AGGAAGGATG AATCCATAAC GCTGGCTCAG 
TTCTTGGTGA CTCCCGAAAG CCAGTCAAGA GGAAAGGGCA GAAAGAGTCC TTTTGAAGAC 
TCTAACTGTG ATAGCTCATC GTCACATAAA AGCTCATCCA GTAGACGTCG CTCAGGAGGT 
AAAGGAAAGC AAACGGGACA GAATGAGAGC GGTGGCTCGA AAGGACAGCA TAAATTGAAA 
TACATTGACA AACCTGTGTT TGACTTGGAA AATGCCCAAG AATTCCCTCC AATGGGTGTA 
CCAAGAACTA TTACCCCAAG GAGGATAACT CCAACTGCAG TATCTTCCAG TAGTGGAGGC 
AGCCGGAGAG TCACCACACT GCTGTCCCCA GCCCCTTCTT GCCAGGGTCA AAATCAGGAA 
ACCAATGCCT TCAATCCCCC TGTAAACCAG ACTGCAGCAA CCAAGAGAGA CACTCATGAG 
TTGAAAAAAT CTTTGGAAAA CGAGAGGGAG TTGCTTAAGC AAGAAAAAAT GAAACAGAAA 
AATCAGCCAG AATGTAGTCC ATTCTCTCAA AATGCATTAG GACAGTCACC AAGAGGAATG 
TCTGTATTGC AATCTCCAAA GGGTATGACT CTAGCTCATA CCCCGACTAA GCTTCTGCGC 
AGTAATTCAG TTTCAAGCAT AACTGAGGCA ATACTACCTG ATGTGGAAAA AGTAACAGGG 
CATGACAAAC TGGATCTCAT AGCAGAGCTC TATGCATCAT TTCTACAAGA GAGCCTTGTT 
CCCAATTTGT CTGTGGAGTT GTACTTCCTG ATTCAGCTGT TGACTGCTAA AGGTAGCAAG 
CTTGATGAGG ATGAGGGAAT ACATTCACTG GAAGAGGACA TTGAAGACAT CAATTATCTC 
TCAACAATCC ACAACTGTGT TTACTTTGCT GCATCAGTGC TCAGCAGGCT GGTTGTAGTG 
TTATCTCTGC TGGATAAGAG CACCTTACGC CTCCTGGCGG ACAACCAAGT GGTCTCTCAG 
TTCAATCCCG AGCTGAAGAC CAGGCTTGAA CAGGTCACCA ATCATTGCCC AACGGAGTCT 
GGTCTTCAGG CTGCCATTAC ATCTCCCATG CAGGTTGTCC CGTTCTTGGC AGATACCGAC 
AACAGGCAGA ACTTTCCAAG TGACACTTCA TTCCACAGTT TCAAGAAACA GAGAGATGGT 
TTCTATAGTC TGATACGAGA CTGGGAAGAT AATCGCAGTA AACCTGGCTG GAAGATGCAG 
GAGAAACTAA CCCACAGGAT CAGGGATTTA ATGAAGCACA TGGATGTGAC AAACTACACC 
CACTTCTCAA GACTGTTCCA GTCACAACTG ATCAAGATGT GCCAAGGTGA TAGCTCAGTG 
GTTGATAGTG GAGATTCTCA GGAATTTAGC TTCATCCAGC AGCTGAGACG GACGAATCCA 
GGAAAGTTTA AAAGATTGGA GGAGCGTTTT TCAACTCCAG CTTCATCCGG TGGTCCATGC 
CCTCCTCCTT CGTTCCATGG ATGTCAGGAA TTCTTTAGAG ATTTCATCCT AGCTACAAAC 
AGTTACATAT TCAACCAGCA TTTGACGGAC AGCTTTGCTA CCAAGCTCAT TGAGCTTAAC 
GATTTACAGT TGCCAATCAC TGATCAGGTT GATATGACTT CTGTTGTTGA TGCGGAGGTA 
AGGGAAAGCT TCAGTTCATG TCTCCTAGCC CTGAGGGTCT TGTCCAAGTT CCTGGGATTT 
CTAGTCTTTC TACCTTACCA GGGTGACAAT AATCTTCCTG ATATCATGAA GGATAATGTT 
ATCATGATGA GGAGTAGGGC ATCTCTTCCC GTGGACATTC TTGGATGTTT GTCTGCATCA 
GAAACCAACC AGCGTCTGAT CCTGACCTTG CCCTGGGTTG TAGAGTATCT TAGTATGATA 
GATGGGCTGG CCTCTCAAAT ACCAGTTTAT TATCAAACAC TCAGTTTATT GGGGGATATT 
CACAGGAGGG TGTCTGTGCA ATGGTCTGAG ACTCCTAGCA ACCATAATTC ACTTCTTATC 
TTGCTTCTCT TGGGATGGCT GTTCGAGAAC CCAGCTCTAC CAAGCAATCT ACATCTGACG 
AGTGGTGTGA GCGTTGAGAG GGGTAGCCAT AATAATAAAC CAGGATCTGA CCAATCTTTA 
GACCCCCTTC CTCTGGTGGA TAGGCTGGTT CTCTATACAT GCTGCCCTTT CCTAGGTGAT 
TTAAGAGGAC TGTTATCCGA GGCTAGTATG GGGGTGAACA ACAGGAATGC TCCGGTCAAG 
AAGATTACTC CTGTAGCAGT GGATGCACCT CAGACTGCCA TTATCACTCA GCACCAAATA 
CAGGTGGAAC TTGAGAAGAA CTTCTTCCAC ATCCACTCCG CTTCCCTCAA GAAGAGCGTG 
GAGTTTGTGA GCGGCAGGGT CGCCTCCAAC TGCATCAAGT CCATCGATGG CTCACTAGTC 
TCTGCAGCCA GGCTCCAGAT TCCAAAGCTA CTCACTGAAG TGCTGGTCTC GTCCAGCAGC 
AGCAAGAAAG AGGCTGGGGC TCTCACAGCC AAGGCTAAGG GAGTTGGAGA GAAGCTGAGC 
CTGGAGACGG CAGAGCTTGC CAGGAAAGAA GCCAAGGAGT TTATCAAGAA GAAGTGTAGG 
TCAGCTCTTG ATGTGTTATT ACCGTGTGAG ACATCTGAGA CAGTCCTGGA CACTGCTTGT 
GCTATCACCA CTAGACTTGC CTTAGAACGA ACAGAGAAAT GGATCACAAC ACAAATACCT 
AGTGCATTAC AACAGGAGGT GACATTGCAG CTACCCAAGT GCCTGAGAGC TCAGAGCACT 
CAACAGATGA AGAAAGAATC AACCAGTAGG AAAATACCAG ACAAGCCAGC CATGCACGAC 
AGACAGGGGG CTTGTCCTTC AACCGTCATT CAAGAACTCA AGGACGGTCT TCATAGAATT 
TTAATTGGAG ACACCGATGG AAACACAGCA GAGGGCATCT GTTCATCCAT CAATAACGTT 
CATATGATGA TCAACAACAG ACAGGACCTA ACACCATTGG CTTACCAGTC CATCCACCAA 
CTCACCATAG AGCTGTGTGT AAGCCTGGCT ATGAGACAAC CAACTCTGCT TCAGACCTCT 
GTCTGTACCT CACCTACAAT CTCCAAAGGG CAAAGGTCCA TCGTTCTACC ACAGGACAGT 
AATACACACG GCAAGAACTG TAGCAGCAGC TTACACAATA CAGAGAATGG CAAACACCAA 
GACAAGAACT TGATATGTTC AACACAGGGA GTAGTTGAAG ATGGAGAGGA GCAGTGCAAT 
AACCAAGAGA GTGGTTTACA CTCACATCTT GGGAGATATG GACAAGAACT TCAGTGCAAT 
ACAAAGGGAG GACAGGGTAC AGAGGAAGGA GCATTCACCA CTCCTGTTCA TAAACATGCA 
TGGAATGGTG ACCAAGGTGA AAGAGATGTA ACTAATGCAG AACAGACAGA AGGAGATGTG 
ACTTTACACC GGTCTAATAC GGATACACTC TTAGAGAGAC TGATGGGTTT GTGGAGGAAT 
GTTTTTTCTT TGAGTATTCC ACCTGGAGCT CTGTTGAGTG CAAGGACAGC ATGGATGATA 
TCACACAGTC ATAACCCACA GGTTTCTTGG CAGTGTCTCA TCCATATAGT GAAGTCCCTT 
CTCGCATGCA ATCTGGTCAG CATTGGGGCT GTCCAATCAG CTTTGAGCAA GATGATGATG 
TCATCATACA TTCCACCAAT TACAGAGGAT GTAGCAAGAA GCCTATTACC TCTGGTCACA 
GATAATGAAA TAGGTGCAGA TCAGAATGCA TTGGACTTGC TGCATGCAGT TTGCTCTGAA 
GCAAGGGAGA ACGAAGATGC CTATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011663039.2
CDS	60..4409
Translation	MAVILEHILSTKLSIQEVLEWSVEGKVEVSNSGFADLLRDTKLQTEFLPFFINSLRDQTAHLVCRGSSAVSSPARTPSSLQKLKRANQTRPQRSGGSKVNRTQLFAADNSKSSWSDKDFTPVSRLTAVEQSPHTPSSSKTNGRHGRNVSWSGGGGDAGSSSRRKTPGSGSRKDESITLAQFLVTPESQSRGKGRKSPFEDSNCDSSSSHKSSSSRRRSGGKGKQTGQNESGGSKGQHKLKYIDKPVFDLENAQEFPPMGVPRTITPRRITPTAVSSSSGGSRRVTTLLSPAPSCQGQNQETNAFNPPVNQTAATKRDTHELKKSLENERELLKQEKMKQKNQPECSPFSQNALGQSPRGMSVLQSPKGMTLAHTPTKLLRSNSVSSITEAILPDVEKVTGHDKLDLIAELYASFLQESLVPNLSVELYFLIQLLTAKGSKLDEDEGIHSLEEDIEDINYLSTIHNCVYFAASVLSRLVVVLSLLDKSTLRLLADNQVVSQFNPELKTRLEQVTNHCPTESGLQAAITSPMQVVPFLADTDNRQNFPSDTSFHSFKKQRDGFYSLIRDWEDNRSKPGWKMQEKLTHRIRDLMKHMDVTNYTHFSRLFQSQLIKMCQGDSSVVDSGDSQEFSFIQQLRRTNPGKFKRLEERFSTPASSGGPCPPPSFHGCQEFFRDFILATNSYIFNQHLTDSFATKLIELNDLQLPITDQVDMTSVVDAEVRESFSSCLLALRVLSKFLGFLVFLPYQGDNNLPDIMKDNVIMMRSRASLPVDILGCLSASETNQRLILTLPWVVEYLSMIDGLASQIPVYYQTLSLLGDIHRRVSVQWSETPSNHNSLLILLLLGWLFENPALPSNLHLTSGVSVERGSHNNKPGSDQSLDPLPLVDRLVLYTCCPFLGDLRGLLSEASMGVNNRNAPVKKITPVAVDAPQTAIITQHQIQVELEKNFFHIHSASLKKSVEFVSGRVASNCIKSIDGSLVSAARLQIPKLLTEVLVSSSSSKKEAGALTAKAKGVGEKLSLETAELARKEAKEFIKKKCRSALDVLLPCETSETVLDTACAITTRLALERTEKWITTQIPSALQQEVTLQLPKCLRAQSTQQMKKESTSRKIPDKPAMHDRQGACPSTVIQELKDGLHRILIGDTDGNTAEGICSSINNVHMMINNRQDLTPLAYQSIHQLTIELCVSLAMRQPTLLQTSVCTSPTISKGQRSIVLPQDSNTHGKNCSSSLHNTENGKHQDKNLICSTQGVVEDGEEQCNNQESGLHSHLGRYGQELQCNTKGGQGTEEGAFTTPVHKHAWNGDQGERDVTNAEQTEGDVTLHRSNTDTLLERLMGLWRNVFSLSIPPGALLSARTAWMISHSHNPQVSWQCLIHIVKSLLACNLVSIGAVQSALSKMMMSSYIPPITEDVARSLLPLVTDNEIGADQNALDLLHAVCSEARENEDAYS

Target ORF information:

RefSeq Version	XM_011664737.2
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011664737.2

ORF Insert Sequence:

ATGGCGGTGA TATTGGAACA CATTTTGTCA ACAAAATTGT CAATACAAGA AGTGTTGGAG 
TGGTCAGTCG AAGGGAAGGT TGAAGTGAGT AACTCTGGAT TTGCAGACTT GTTGAGGGAT 
ACCAAACTGC AAACAGAATT TCTACCATTC TTCATCAATA GCTTGCGGGA TCAGACAGCC 
CACTTAGTAT GCAGGGGATC ATCTGCTGTG TCTTCCCCAG CGAGGACGCC AAGCAGCTTA 
CAAAAACTAA AAAGAGCCAA TCAGACACGC CCTCAGCGCA GTGGAGGTTC AAAGGTCAAT 
CGTACACAGC TGTTTGCTGC TGATAACAGC AAGTCCAGCT GGTCAGACAA AGACTTTACA 
CCCGTTTCAA GACTAACAGC AGTAGAGCAG TCGCCGCACA CTCCATCGTC ATCTAAGACA 
AATGGAAGAC ACGGAAGAAA TGTGTCTTGG AGTGGTGGTG GCGGTGATGC TGGCTCCAGC 
TCCAGGAGGA AGACTCCCGG ATCAGGATCT AGGAAGGATG AATCCATAAC GCTGGCTCAG 
TTCTTGGTGA CTCCCGAAAG CCAGTCAAGA GGAAAGGGCA GAAAGAGTCC TTTTGAAGAC 
TCTAACTGTG ATAGCTCATC GTCACATAAA AGCTCATCCA GTAGACGTCG CTCAGGAGGT 
AAAGGAAAGC AAACGGGACA GAATGAGAGC GGTGGCTCGA AAGGACAGCA TAAATTGAAA 
TACATTGACA AACCTGTGTT TGACTTGGAA AATGCCCAAG AATTCCCTCC AATGGGTGTA 
CCAAGAACTA TTACCCCAAG GAGGATAACT CCAACTGCAG TATCTTCCAG TAGTGGAGGC 
AGCCGGAGAG TCACCACACT GCTGTCCCCA GCCCCTTCTT GCCAGGGTCA AAATCAGGAA 
ACCAATGCCT TCAATCCCCC TGTAAACCAG ACTGCAGCAA CCAAGAGAGA CACTCATGAG 
TTGAAAAAAT CTTTGGAAAA CGAGAGGGAG TTGCTTAAGC AAGAAAAAAT GAAACAGAAA 
AATCAGCCAG AATGTAGTCC ATTCTCTCAA AATGCATTAG GACAGTCACC AAGAGGAATG 
TCTGTATTGC AATCTCCAAA GGGTATGACT CTAGCTCATA CCCCGACTAA GCTTCTGCGC 
AGTAATTCAG TTTCAAGCAT AACTGAGGCA ATACTACCTG ATGTGGAAAA AGTAACAGGG 
CATGACAAAC TGGATCTCAT AGCAGAGCTC TATGCATCAT TTCTACAAGA GAGCCTTGTT 
CCCAATTTGT CTGTGGAGTT GTACTTCCTG ATTCAGCTGT TGACTGCTAA AGGTAGCAAG 
CTTGATGAGG ATGAGGGAAT ACATTCACTG GAAGAGGACA TTGAAGACAT CAATTATCTC 
TCAACAATCC ACAACTGTGT TTACTTTGCT GCATCAGTGC TCAGCAGGCT GGTTGTAGTG 
TTATCTCTGC TGGATAAGAG CACCTTACGC CTCCTGGCGG ACAACCAAGT GGTCTCTCAG 
TTCAATCCCG AGCTGAAGAC CAGGCTTGAA CAGGTCACCA ATCATTGCCC AACGGAGTCT 
GGTCTTCAGG CTGCCATTAC ATCTCCCATG CAGGTTGTCC CGTTCTTGGC AGATACCGAC 
AACAGGCAGA ACTTTCCAAG TGACACTTCA TTCCACAGTT TCAAGAAACA GAGAGATGGT 
TTCTATAGTC TGATACGAGA CTGGGAAGAT AATCGCAGTA AACCTGGCTG GAAGATGCAG 
GAGAAACTAA CCCACAGGAT CAGGGATTTA ATGAAGCACA TGGATGTGAC AAACTACACC 
CACTTCTCAA GACTGTTCCA GTCACAACTG ATCAAGATGT GCCAAGGTGA TAGCTCAGTG 
GTTGATAGTG GAGATTCTCA GGAATTTAGC TTCATCCAGC AGCTGAGACG GACGAATCCA 
GGAAAGTTTA AAAGATTGGA GGAGCGTTTT TCAACTCCAG CTTCATCCGG TGGTCCATGC 
CCTCCTCCTT CGTTCCATGG ATGTCAGGAA TTCTTTAGAG ATTTCATCCT AGCTACAAAC 
AGTTACATAT TCAACCAGCA TTTGACGGAC AGCTTTGCTA CCAAGCTCAT TGAGCTTAAC 
GATTTACAGT TGCCAATCAC TGATCAGGTT GATATGACTT CTGTTGTTGA TGCGGAGGTA 
AGGGAAAGCT TCAGTTCATG TCTCCTAGCC CTGAGGGTCT TGTCCAAGTT CCTGGGATTT 
CTAGTCTTTC TACCTTACCA GGGTGACAAT AATCTTCCTG ATATCATGAA GGATAATGTT 
ATCATGATGA GGAGTAGGGC ATCTCTTCCC GTGGACATTC TTGGATGTTT GTCTGCATCA 
GAAACCAACC AGCGTCTGAT CCTGACCTTG CCCTGGGTTG TAGAGTATCT TAGTATGATA 
GATGGGCTGG CCTCTCAAAT ACCAGTTTAT TATCAAACAC TCAGTTTATT GGGGGATATT 
CACAGGAGGG TGTCTGTGCA ATGGTCTGAG ACTCCTAGCA ACCATAATTC ACTTCTTATC 
TTGCTTCTCT TGGGATGGCT GTTCGAGAAC CCAGCTCTAC CAAGCAATCT ACATCTGACG 
AGTGGTGTGA GCGTTGAGAG GGGTAGCCAT AATAATAAAC CAGGATCTGA CCAATCTTTA 
GACCCCCTTC CTCTGGTGGA TAGGCTGGTT CTCTATACAT GCTGCCCTTT CCTAGGTGAT 
TTAAGAGGAC TGTTATCCGA GGCTAGTATG GGGGTGAACA ACAGGAATGC TCCGGTCAAG 
AAGATTACTC CTGTAGCAGT GGATGCACCT CAGACTGCCA TTATCACTCA GCACCAAATA 
CAGGTGGAAC TTGAGAAGAA CTTCTTCCAC ATCCACTCCG CTTCCCTCAA GAAGAGCGTG 
GAGTTTGTGA GCGGCAGGGT CGCCTCCAAC TGCATCAAGT CCATCGATGG CTCACTAGTC 
TCTGCAGCCA GGCTCCAGAT TCCAAAGCTA CTCACTGAAG TGCTGGTCTC GTCCAGCAGC 
AGCAAGAAAG AGGCTGGGGC TCTCACAGCC AAGGCTAAGG GAGTTGGAGA GAAGCTGAGC 
CTGGAGACGG CAGAGCTTGC CAGGAAAGAA GCCAAGGAGT TTATCAAGAA GAAGTGTAGG 
TCAGCTCTTG ATGTGTTATT ACCGTGTGAG ACATCTGAGA CAGTCCTGGA CACTGCTTGT 
GCTATCACCA CTAGACTTGC CTTAGAACGA ACAGAGAAAT GGATCACAAC ACAAATACCT 
AGTGCATTAC AACAGGAGGT GACATTGCAG CTACCCAAGT GCCTGAGAGC TCAGAGCACT 
CAACAGATGA AGAAAGAATC AACCAGTAGG AAAATACCAG ACAAGCCAGC CATGCACGAC 
AGACAGGGGG CTTGTCCTTC AACCGTCATT CAAGAACTCA AGGACGGTCT TCATAGAATT 
TTAATTGGAG ACACCGATGG AAACACAGCA GAGGGCATCT GTTCATCCAT CAATAACGTT 
CATATGATGA TCAACAACAG ACAGGACCTA ACACCATTGG CTTACCAGTC CATCCACCAA 
CTCACCATAG AGCTGTGTGT AAGCCTGGCT ATGAGACAAC CAACTCTGCT TCAGACCTCT 
GTCTGTACCT CACCTACAAT CTCCAAAGGG CAAAGGTCCA TCGTTCTACC ACAGGACAGT 
AATACACACG GCAAGAACTG TAGCAGCAGC TTACACAATA CAGAGAATGG CAAACACCAA 
GACAAGAACT TGATATGTTC AACACAGGGA GTAGTTGAAG ATGGAGAGGA GCAGTGCAAT 
AACCAAGAGA GTGGTTTACA CTCACATCTT GGGAGATATG GACAAGAACT TCAGTGCAAT 
ACAAAGGGAG GACAGGGTAC AGAGGAAGGA GCATTCACCA CTCCTGTTCA TAAACATGCA 
TGGAATGGTG ACCAAGGTGA AAGAGATGTA ACTAATGCAG AACAGACAGA AGGAGATGTG 
ACTTTACACC GGTCTAATAC GGATACACTC TTAGAGAGAC TGATGGGTTT GTGGAGGAAT 
GTTTTTTCTT TGAGTATTCC ACCTGGAGCT CTGTTGAGTG CAAGGACAGC ATGGATGATA 
TCACACAGTC ATAACCCACA GGTTTCTTGG CAGTGTCTCA TCCATATAGT GAAGTCCCTT 
CTCGCATGCA ATCTGGTCAG CATTGGGGCT GTCCAATCAG CTTTGAGCAA GATGATGATG 
TCATCATACA TTCCACCAAT TACAGAGGAT GTAGCAAGAA GCCTATTACC TCTGGTCACA 
GATAATGAAA TAGGTGCAGA TCAGAATGCA TTGGACTTGC TGCATGCAGT TTGCTCTGAA 
GCAAGGGAGA ACGAAGATGC CTATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC579713 ( XM_011664739.2 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011663041.2 Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X2, mRNA.

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CloneID	OSd65760
Clone ID Related Accession (Same CDS sequence)	XM_011664739.2
Accession Version	XM_011664739.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	codanin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 27, 2019 this sequence version replaced XM_011664739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGA TATTGGAACA CATTTTGTCA ACAAAATTGT CAATACAAGA AGTGTTGGAG 
TGGTCAGTCG AAGGGAAGGT TGAAAACTCT GGATTTGCAG ACTTGTTGAG GGATACCAAA 
CTGCAAACAG AATTTCTACC ATTCTTCATC AATAGCTTGC GGGATCAGAC AGCCCACTTA 
GTATGCAGGG GATCATCTGC TGTGTCTTCC CCAGCGAGGA CGCCAAGCAG CTTACAAAAA 
CTAAAAAGAG CCAATCAGAC ACGCCCTCAG CGCAGTGGAG GTTCAAAGGT CAATCGTACA 
CAGCTGTTTG CTGCTGATAA CAGCAAGTCC AGCTGGTCAG ACAAAGACTT TACACCCGTT 
TCAAGACTAA CAGCAGTAGA GCAGTCGCCG CACACTCCAT CGTCATCTAA GACAAATGGA 
AGACACGGAA GAAATGTGTC TTGGAGTGGT GGTGGCGGTG ATGCTGGCTC CAGCTCCAGG 
AGGAAGACTC CCGGATCAGG ATCTAGGAAG GATGAATCCA TAACGCTGGC TCAGTTCTTG 
GTGACTCCCG AAAGCCAGTC AAGAGGAAAG GGCAGAAAGA GTCCTTTTGA AGACTCTAAC 
TGTGATAGCT CATCGTCACA TAAAAGCTCA TCCAGTAGAC GTCGCTCAGG AGGTAAAGGA 
AAGCAAACGG GACAGAATGA GAGCGGTGGC TCGAAAGGAC AGCATAAATT GAAATACATT 
GACAAACCTG TGTTTGACTT GGAAAATGCC CAAGAATTCC CTCCAATGGG TGTACCAAGA 
ACTATTACCC CAAGGAGGAT AACTCCAACT GCAGTATCTT CCAGTAGTGG AGGCAGCCGG 
AGAGTCACCA CACTGCTGTC CCCAGCCCCT TCTTGCCAGG GTCAAAATCA GGAAACCAAT 
GCCTTCAATC CCCCTGTAAA CCAGACTGCA GCAACCAAGA GAGACACTCA TGAGTTGAAA 
AAATCTTTGG AAAACGAGAG GGAGTTGCTT AAGCAAGAAA AAATGAAACA GAAAAATCAG 
CCAGAATGTA GTCCATTCTC TCAAAATGCA TTAGGACAGT CACCAAGAGG AATGTCTGTA 
TTGCAATCTC CAAAGGGTAT GACTCTAGCT CATACCCCGA CTAAGCTTCT GCGCAGTAAT 
TCAGTTTCAA GCATAACTGA GGCAATACTA CCTGATGTGG AAAAAGTAAC AGGGCATGAC 
AAACTGGATC TCATAGCAGA GCTCTATGCA TCATTTCTAC AAGAGAGCCT TGTTCCCAAT 
TTGTCTGTGG AGTTGTACTT CCTGATTCAG CTGTTGACTG CTAAAGGTAG CAAGCTTGAT 
GAGGATGAGG GAATACATTC ACTGGAAGAG GACATTGAAG ACATCAATTA TCTCTCAACA 
ATCCACAACT GTGTTTACTT TGCTGCATCA GTGCTCAGCA GGCTGGTTGT AGTGTTATCT 
CTGCTGGATA AGAGCACCTT ACGCCTCCTG GCGGACAACC AAGTGGTCTC TCAGTTCAAT 
CCCGAGCTGA AGACCAGGCT TGAACAGGTC ACCAATCATT GCCCAACGGA GTCTGGTCTT 
CAGGCTGCCA TTACATCTCC CATGCAGGTT GTCCCGTTCT TGGCAGATAC CGACAACAGG 
CAGAACTTTC CAAGTGACAC TTCATTCCAC AGTTTCAAGA AACAGAGAGA TGGTTTCTAT 
AGTCTGATAC GAGACTGGGA AGATAATCGC AGTAAACCTG GCTGGAAGAT GCAGGAGAAA 
CTAACCCACA GGATCAGGGA TTTAATGAAG CACATGGATG TGACAAACTA CACCCACTTC 
TCAAGACTGT TCCAGTCACA ACTGATCAAG ATGTGCCAAG GTGATAGCTC AGTGGTTGAT 
AGTGGAGATT CTCAGGAATT TAGCTTCATC CAGCAGCTGA GACGGACGAA TCCAGGAAAG 
TTTAAAAGAT TGGAGGAGCG TTTTTCAACT CCAGCTTCAT CCGGTGGTCC ATGCCCTCCT 
CCTTCGTTCC ATGGATGTCA GGAATTCTTT AGAGATTTCA TCCTAGCTAC AAACAGTTAC 
ATATTCAACC AGCATTTGAC GGACAGCTTT GCTACCAAGC TCATTGAGCT TAACGATTTA 
CAGTTGCCAA TCACTGATCA GGTTGATATG ACTTCTGTTG TTGATGCGGA GGTAAGGGAA 
AGCTTCAGTT CATGTCTCCT AGCCCTGAGG GTCTTGTCCA AGTTCCTGGG ATTTCTAGTC 
TTTCTACCTT ACCAGGGTGA CAATAATCTT CCTGATATCA TGAAGGATAA TGTTATCATG 
ATGAGGAGTA GGGCATCTCT TCCCGTGGAC ATTCTTGGAT GTTTGTCTGC ATCAGAAACC 
AACCAGCGTC TGATCCTGAC CTTGCCCTGG GTTGTAGAGT ATCTTAGTAT GATAGATGGG 
CTGGCCTCTC AAATACCAGT TTATTATCAA ACACTCAGTT TATTGGGGGA TATTCACAGG 
AGGGTGTCTG TGCAATGGTC TGAGACTCCT AGCAACCATA ATTCACTTCT TATCTTGCTT 
CTCTTGGGAT GGCTGTTCGA GAACCCAGCT CTACCAAGCA ATCTACATCT GACGAGTGGT 
GTGAGCGTTG AGAGGGGTAG CCATAATAAT AAACCAGGAT CTGACCAATC TTTAGACCCC 
CTTCCTCTGG TGGATAGGCT GGTTCTCTAT ACATGCTGCC CTTTCCTAGG TGATTTAAGA 
GGACTGTTAT CCGAGGCTAG TATGGGGGTG AACAACAGGA ATGCTCCGGT CAAGAAGATT 
ACTCCTGTAG CAGTGGATGC ACCTCAGACT GCCATTATCA CTCAGCACCA AATACAGGTG 
GAACTTGAGA AGAACTTCTT CCACATCCAC TCCGCTTCCC TCAAGAAGAG CGTGGAGTTT 
GTGAGCGGCA GGGTCGCCTC CAACTGCATC AAGTCCATCG ATGGCTCACT AGTCTCTGCA 
GCCAGGCTCC AGATTCCAAA GCTACTCACT GAAGTGCTGG TCTCGTCCAG CAGCAGCAAG 
AAAGAGGCTG GGGCTCTCAC AGCCAAGGCT AAGGGAGTTG GAGAGAAGCT GAGCCTGGAG 
ACGGCAGAGC TTGCCAGGAA AGAAGCCAAG GAGTTTATCA AGAAGAAGTG TAGGTCAGCT 
CTTGATGTGT TATTACCGTG TGAGACATCT GAGACAGTCC TGGACACTGC TTGTGCTATC 
ACCACTAGAC TTGCCTTAGA ACGAACAGAG AAATGGATCA CAACACAAAT ACCTAGTGCA 
TTACAACAGG AGGTGACATT GCAGCTACCC AAGTGCCTGA GAGCTCAGAG CACTCAACAG 
ATGAAGAAAG AATCAACCAG TAGGAAAATA CCAGACAAGC CAGCCATGCA CGACAGACAG 
GGGGCTTGTC CTTCAACCGT CATTCAAGAA CTCAAGGACG GTCTTCATAG AATTTTAATT 
GGAGACACCG ATGGAAACAC AGCAGAGGGC ATCTGTTCAT CCATCAATAA CGTTCATATG 
ATGATCAACA ACAGACAGGA CCTAACACCA TTGGCTTACC AGTCCATCCA CCAACTCACC 
ATAGAGCTGT GTGTAAGCCT GGCTATGAGA CAACCAACTC TGCTTCAGAC CTCTGTCTGT 
ACCTCACCTA CAATCTCCAA AGGGCAAAGG TCCATCGTTC TACCACAGGA CAGTAATACA 
CACGGCAAGA ACTGTAGCAG CAGCTTACAC AATACAGAGA ATGGCAAACA CCAAGACAAG 
AACTTGATAT GTTCAACACA GGGAGTAGTT GAAGATGGAG AGGAGCAGTG CAATAACCAA 
GAGAGTGGTT TACACTCACA TCTTGGGAGA TATGGACAAG AACTTCAGTG CAATACAAAG 
GGAGGACAGG GTACAGAGGA AGGAGCATTC ACCACTCCTG TTCATAAACA TGCATGGAAT 
GGTGACCAAG GTGAAAGAGA TGTAACTAAT GCAGAACAGA CAGAAGGAGA TGTGACTTTA 
CACCGGTCTA ATACGGATAC ACTCTTAGAG AGACTGATGG GTTTGTGGAG GAATGTTTTT 
TCTTTGAGTA TTCCACCTGG AGCTCTGTTG AGTGCAAGGA CAGCATGGAT GATATCACAC 
AGTCATAACC CACAGGTTTC TTGGCAGTGT CTCATCCATA TAGTGAAGTC CCTTCTCGCA 
TGCAATCTGG TCAGCATTGG GGCTGTCCAA TCAGCTTTGA GCAAGATGAT GATGTCATCA 
TACATTCCAC CAATTACAGA GGATGTAGCA AGAAGCCTAT TACCTCTGGT CACAGATAAT 
GAAATAGGTG CAGATCAGAA TGCATTGGAC TTGCTGCATG CAGTTTGCTC TGAAGCAAGG 
GAGAACGAAG ATGCCTATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011663041.2
CDS	60..4403
Translation	MAVILEHILSTKLSIQEVLEWSVEGKVENSGFADLLRDTKLQTEFLPFFINSLRDQTAHLVCRGSSAVSSPARTPSSLQKLKRANQTRPQRSGGSKVNRTQLFAADNSKSSWSDKDFTPVSRLTAVEQSPHTPSSSKTNGRHGRNVSWSGGGGDAGSSSRRKTPGSGSRKDESITLAQFLVTPESQSRGKGRKSPFEDSNCDSSSSHKSSSSRRRSGGKGKQTGQNESGGSKGQHKLKYIDKPVFDLENAQEFPPMGVPRTITPRRITPTAVSSSSGGSRRVTTLLSPAPSCQGQNQETNAFNPPVNQTAATKRDTHELKKSLENERELLKQEKMKQKNQPECSPFSQNALGQSPRGMSVLQSPKGMTLAHTPTKLLRSNSVSSITEAILPDVEKVTGHDKLDLIAELYASFLQESLVPNLSVELYFLIQLLTAKGSKLDEDEGIHSLEEDIEDINYLSTIHNCVYFAASVLSRLVVVLSLLDKSTLRLLADNQVVSQFNPELKTRLEQVTNHCPTESGLQAAITSPMQVVPFLADTDNRQNFPSDTSFHSFKKQRDGFYSLIRDWEDNRSKPGWKMQEKLTHRIRDLMKHMDVTNYTHFSRLFQSQLIKMCQGDSSVVDSGDSQEFSFIQQLRRTNPGKFKRLEERFSTPASSGGPCPPPSFHGCQEFFRDFILATNSYIFNQHLTDSFATKLIELNDLQLPITDQVDMTSVVDAEVRESFSSCLLALRVLSKFLGFLVFLPYQGDNNLPDIMKDNVIMMRSRASLPVDILGCLSASETNQRLILTLPWVVEYLSMIDGLASQIPVYYQTLSLLGDIHRRVSVQWSETPSNHNSLLILLLLGWLFENPALPSNLHLTSGVSVERGSHNNKPGSDQSLDPLPLVDRLVLYTCCPFLGDLRGLLSEASMGVNNRNAPVKKITPVAVDAPQTAIITQHQIQVELEKNFFHIHSASLKKSVEFVSGRVASNCIKSIDGSLVSAARLQIPKLLTEVLVSSSSSKKEAGALTAKAKGVGEKLSLETAELARKEAKEFIKKKCRSALDVLLPCETSETVLDTACAITTRLALERTEKWITTQIPSALQQEVTLQLPKCLRAQSTQQMKKESTSRKIPDKPAMHDRQGACPSTVIQELKDGLHRILIGDTDGNTAEGICSSINNVHMMINNRQDLTPLAYQSIHQLTIELCVSLAMRQPTLLQTSVCTSPTISKGQRSIVLPQDSNTHGKNCSSSLHNTENGKHQDKNLICSTQGVVEDGEEQCNNQESGLHSHLGRYGQELQCNTKGGQGTEEGAFTTPVHKHAWNGDQGERDVTNAEQTEGDVTLHRSNTDTLLERLMGLWRNVFSLSIPPGALLSARTAWMISHSHNPQVSWQCLIHIVKSLLACNLVSIGAVQSALSKMMMSSYIPPITEDVARSLLPLVTDNEIGADQNALDLLHAVCSEARENEDAYS

Target ORF information:

RefSeq Version	XM_011664739.2
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus codanin-1 (LOC579713), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011664739.2

ORF Insert Sequence:

ATGGCGGTGA TATTGGAACA CATTTTGTCA ACAAAATTGT CAATACAAGA AGTGTTGGAG 
TGGTCAGTCG AAGGGAAGGT TGAAAACTCT GGATTTGCAG ACTTGTTGAG GGATACCAAA 
CTGCAAACAG AATTTCTACC ATTCTTCATC AATAGCTTGC GGGATCAGAC AGCCCACTTA 
GTATGCAGGG GATCATCTGC TGTGTCTTCC CCAGCGAGGA CGCCAAGCAG CTTACAAAAA 
CTAAAAAGAG CCAATCAGAC ACGCCCTCAG CGCAGTGGAG GTTCAAAGGT CAATCGTACA 
CAGCTGTTTG CTGCTGATAA CAGCAAGTCC AGCTGGTCAG ACAAAGACTT TACACCCGTT 
TCAAGACTAA CAGCAGTAGA GCAGTCGCCG CACACTCCAT CGTCATCTAA GACAAATGGA 
AGACACGGAA GAAATGTGTC TTGGAGTGGT GGTGGCGGTG ATGCTGGCTC CAGCTCCAGG 
AGGAAGACTC CCGGATCAGG ATCTAGGAAG GATGAATCCA TAACGCTGGC TCAGTTCTTG 
GTGACTCCCG AAAGCCAGTC AAGAGGAAAG GGCAGAAAGA GTCCTTTTGA AGACTCTAAC 
TGTGATAGCT CATCGTCACA TAAAAGCTCA TCCAGTAGAC GTCGCTCAGG AGGTAAAGGA 
AAGCAAACGG GACAGAATGA GAGCGGTGGC TCGAAAGGAC AGCATAAATT GAAATACATT 
GACAAACCTG TGTTTGACTT GGAAAATGCC CAAGAATTCC CTCCAATGGG TGTACCAAGA 
ACTATTACCC CAAGGAGGAT AACTCCAACT GCAGTATCTT CCAGTAGTGG AGGCAGCCGG 
AGAGTCACCA CACTGCTGTC CCCAGCCCCT TCTTGCCAGG GTCAAAATCA GGAAACCAAT 
GCCTTCAATC CCCCTGTAAA CCAGACTGCA GCAACCAAGA GAGACACTCA TGAGTTGAAA 
AAATCTTTGG AAAACGAGAG GGAGTTGCTT AAGCAAGAAA AAATGAAACA GAAAAATCAG 
CCAGAATGTA GTCCATTCTC TCAAAATGCA TTAGGACAGT CACCAAGAGG AATGTCTGTA 
TTGCAATCTC CAAAGGGTAT GACTCTAGCT CATACCCCGA CTAAGCTTCT GCGCAGTAAT 
TCAGTTTCAA GCATAACTGA GGCAATACTA CCTGATGTGG AAAAAGTAAC AGGGCATGAC 
AAACTGGATC TCATAGCAGA GCTCTATGCA TCATTTCTAC AAGAGAGCCT TGTTCCCAAT 
TTGTCTGTGG AGTTGTACTT CCTGATTCAG CTGTTGACTG CTAAAGGTAG CAAGCTTGAT 
GAGGATGAGG GAATACATTC ACTGGAAGAG GACATTGAAG ACATCAATTA TCTCTCAACA 
ATCCACAACT GTGTTTACTT TGCTGCATCA GTGCTCAGCA GGCTGGTTGT AGTGTTATCT 
CTGCTGGATA AGAGCACCTT ACGCCTCCTG GCGGACAACC AAGTGGTCTC TCAGTTCAAT 
CCCGAGCTGA AGACCAGGCT TGAACAGGTC ACCAATCATT GCCCAACGGA GTCTGGTCTT 
CAGGCTGCCA TTACATCTCC CATGCAGGTT GTCCCGTTCT TGGCAGATAC CGACAACAGG 
CAGAACTTTC CAAGTGACAC TTCATTCCAC AGTTTCAAGA AACAGAGAGA TGGTTTCTAT 
AGTCTGATAC GAGACTGGGA AGATAATCGC AGTAAACCTG GCTGGAAGAT GCAGGAGAAA 
CTAACCCACA GGATCAGGGA TTTAATGAAG CACATGGATG TGACAAACTA CACCCACTTC 
TCAAGACTGT TCCAGTCACA ACTGATCAAG ATGTGCCAAG GTGATAGCTC AGTGGTTGAT 
AGTGGAGATT CTCAGGAATT TAGCTTCATC CAGCAGCTGA GACGGACGAA TCCAGGAAAG 
TTTAAAAGAT TGGAGGAGCG TTTTTCAACT CCAGCTTCAT CCGGTGGTCC ATGCCCTCCT 
CCTTCGTTCC ATGGATGTCA GGAATTCTTT AGAGATTTCA TCCTAGCTAC AAACAGTTAC 
ATATTCAACC AGCATTTGAC GGACAGCTTT GCTACCAAGC TCATTGAGCT TAACGATTTA 
CAGTTGCCAA TCACTGATCA GGTTGATATG ACTTCTGTTG TTGATGCGGA GGTAAGGGAA 
AGCTTCAGTT CATGTCTCCT AGCCCTGAGG GTCTTGTCCA AGTTCCTGGG ATTTCTAGTC 
TTTCTACCTT ACCAGGGTGA CAATAATCTT CCTGATATCA TGAAGGATAA TGTTATCATG 
ATGAGGAGTA GGGCATCTCT TCCCGTGGAC ATTCTTGGAT GTTTGTCTGC ATCAGAAACC 
AACCAGCGTC TGATCCTGAC CTTGCCCTGG GTTGTAGAGT ATCTTAGTAT GATAGATGGG 
CTGGCCTCTC AAATACCAGT TTATTATCAA ACACTCAGTT TATTGGGGGA TATTCACAGG 
AGGGTGTCTG TGCAATGGTC TGAGACTCCT AGCAACCATA ATTCACTTCT TATCTTGCTT 
CTCTTGGGAT GGCTGTTCGA GAACCCAGCT CTACCAAGCA ATCTACATCT GACGAGTGGT 
GTGAGCGTTG AGAGGGGTAG CCATAATAAT AAACCAGGAT CTGACCAATC TTTAGACCCC 
CTTCCTCTGG TGGATAGGCT GGTTCTCTAT ACATGCTGCC CTTTCCTAGG TGATTTAAGA 
GGACTGTTAT CCGAGGCTAG TATGGGGGTG AACAACAGGA ATGCTCCGGT CAAGAAGATT 
ACTCCTGTAG CAGTGGATGC ACCTCAGACT GCCATTATCA CTCAGCACCA AATACAGGTG 
GAACTTGAGA AGAACTTCTT CCACATCCAC TCCGCTTCCC TCAAGAAGAG CGTGGAGTTT 
GTGAGCGGCA GGGTCGCCTC CAACTGCATC AAGTCCATCG ATGGCTCACT AGTCTCTGCA 
GCCAGGCTCC AGATTCCAAA GCTACTCACT GAAGTGCTGG TCTCGTCCAG CAGCAGCAAG 
AAAGAGGCTG GGGCTCTCAC AGCCAAGGCT AAGGGAGTTG GAGAGAAGCT GAGCCTGGAG 
ACGGCAGAGC TTGCCAGGAA AGAAGCCAAG GAGTTTATCA AGAAGAAGTG TAGGTCAGCT 
CTTGATGTGT TATTACCGTG TGAGACATCT GAGACAGTCC TGGACACTGC TTGTGCTATC 
ACCACTAGAC TTGCCTTAGA ACGAACAGAG AAATGGATCA CAACACAAAT ACCTAGTGCA 
TTACAACAGG AGGTGACATT GCAGCTACCC AAGTGCCTGA GAGCTCAGAG CACTCAACAG 
ATGAAGAAAG AATCAACCAG TAGGAAAATA CCAGACAAGC CAGCCATGCA CGACAGACAG 
GGGGCTTGTC CTTCAACCGT CATTCAAGAA CTCAAGGACG GTCTTCATAG AATTTTAATT 
GGAGACACCG ATGGAAACAC AGCAGAGGGC ATCTGTTCAT CCATCAATAA CGTTCATATG 
ATGATCAACA ACAGACAGGA CCTAACACCA TTGGCTTACC AGTCCATCCA CCAACTCACC 
ATAGAGCTGT GTGTAAGCCT GGCTATGAGA CAACCAACTC TGCTTCAGAC CTCTGTCTGT 
ACCTCACCTA CAATCTCCAA AGGGCAAAGG TCCATCGTTC TACCACAGGA CAGTAATACA 
CACGGCAAGA ACTGTAGCAG CAGCTTACAC AATACAGAGA ATGGCAAACA CCAAGACAAG 
AACTTGATAT GTTCAACACA GGGAGTAGTT GAAGATGGAG AGGAGCAGTG CAATAACCAA 
GAGAGTGGTT TACACTCACA TCTTGGGAGA TATGGACAAG AACTTCAGTG CAATACAAAG 
GGAGGACAGG GTACAGAGGA AGGAGCATTC ACCACTCCTG TTCATAAACA TGCATGGAAT 
GGTGACCAAG GTGAAAGAGA TGTAACTAAT GCAGAACAGA CAGAAGGAGA TGTGACTTTA 
CACCGGTCTA ATACGGATAC ACTCTTAGAG AGACTGATGG GTTTGTGGAG GAATGTTTTT 
TCTTTGAGTA TTCCACCTGG AGCTCTGTTG AGTGCAAGGA CAGCATGGAT GATATCACAC 
AGTCATAACC CACAGGTTTC TTGGCAGTGT CTCATCCATA TAGTGAAGTC CCTTCTCGCA 
TGCAATCTGG TCAGCATTGG GGCTGTCCAA TCAGCTTTGA GCAAGATGAT GATGTCATCA 
TACATTCCAC CAATTACAGA GGATGTAGCA AGAAGCCTAT TACCTCTGGT CACAGATAAT 
GAAATAGGTG CAGATCAGAA TGCATTGGAC TTGCTGCATG CAGTTTGCTC TGAAGCAAGG 
GAGAACGAAG ATGCCTATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC579713 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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