LOC579010 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC579010
Entrez Gene ID	579010
Full Name	nodal
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001098449.1	NP_001091919.1	nodal precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Oral-aboral axis specification in the sea urchin embryo, IV: hypoxia radializes embryos by preventing the initial spatialization of nodal activity.
Coffman JA, Wessels A, DeSchiffart C, Rydlizky K
Developmental biology386(2)302-7(2014 Feb)

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Tu Q, Cameron RA, Worley KC, Gibbs RA, Davidson EH
Genome research22(10)2079-87(2012 Oct)

Axial patterning interactions in the sea urchin embryo: suppression of nodal by Wnt1 signaling.
Wei Z, Range R, Angerer R, Angerer L
Development (Cambridge, England)139(9)1662-9(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC579010 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC579010 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSd00056	NM_001098449.1 Latest version!	Strongylocentrotus purpuratus nodal (LOC579010), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC579010 ( NM_001098449.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> NP_001091919.1 Strongylocentrotus purpuratus nodal (LOC579010), mRNA.

Price & Availability↑

CloneID	OSd00056
Clone ID Related Accession (Same CDS sequence)	NM_001098449.1
Accession Version	NM_001098449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498233600000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	nodal precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF036514.1. On or before Jun 3, 2007 this sequence version replaced XM_774841.2, XM_001177393.1. ##Evidence-Data-START## Transcript exon combination :: EF036514.1, JT118949.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01103178, SAMN01103179 [ECO:0000348] ##Evidence-Data-END##

ATGCATCGTT CATCGGATCT CCACTTGGCG GCTGTCGTCT GCTTGTTTTT TGTCACATCA 
GTGAGTGCCT TTTTTGCTCC ATCCCTACAT GAGACGGCCT CATCTCTGAG CGACGACGTC 
GTGGCCCCAG GTCGCACCAG ACGGAATACT CTACCTCTTA TCACACCGGA CGCCCTCATC 
AGCGTCGATC AGCATACGAA CTCGTCGACG ATGTACATGG TGGAATTGTT TGAGTCTCTC 
TACACTGGAC ATAATCTTGT CAACCCATCC AAGGAAGAAT GCCAAGCCAT TGAGGAGGCG 
GACACAGTAC GGAACTACAT TGGAGCCATT CAAGATGTCA AATCTTCTTC CGACCTTGTG 
ATGACATGGG TGACCTCTTC CCCGACGTTT GACCAGCCCG CCAACGAAGC TGTCCATCTC 
AGCGAGCTCC GAGTTGAACT CAATGCCACC TCAGAGCAGT CGCATCGTTT CGTCATCCAA 
CTCCACCAGT GGGTCAACGT CTCCTGCGAC GACGGCATGT GTCAGCAACG CAAGCTGATT 
CATTCAAGGG ACATCGACGC TCGCCACGAC GACTTCGATG GCAGGGAGGT GTTCGACATC 
ACCAAGATCG TCAAACAGTG GATCGCAACG ACCAAAGACG CGACGTCCCC AATGGAGTAC 
AGCATCGAGC TACGCGCCAA AGCACTCATC GAATCAGAAG CTGACCACGA CAAGAATGTT 
CTCAAAGCCC TTCTTGCAGC CGACATTCAA ACAGAAGATG TCTTGGATGA TATCGCACTT 
GACTCTGACG ATGTTTCGAG CGAAGACCAC GGAGCTACCA ATCAGCTCTC CGTTACTGAC 
GTGACGCTCG TCGTCTTTTC TCGTGCCCCT ACCTCTCCGA TCATTCTCTC CGATGCTCAC 
GGTTCGGTGC GTACCCGTGC TCGACGATCA ACAGATGAAG CTCGTCGCCG CAAGAAGCAG 
AAAGATCTAC GCAAGCTTCA ACGCGACGAA CACAAGCACA AAATGGACGA GAAGCTTCGC 
TTGGAACGAG AGCAATCTGG GCCTTGTCGT CGAATTGATA TGGACGTTGA TTTCGGTCGA 
ATCGGCTGGG ACGAATGGAT CATCTACCCA AAGCAGTTCA ACGCCTACCG TTGCGTCGGT 
ACGTGTAAGG GACCTCTTGA CAGCCGCGAC AACCCAAGCA ACCACGCAGT CATGCAGGAC 
TTGGTTCGAC TCCACCAACC CGAGAGACGG ACACCTGAAC CTTGCTGCGT ACCAACCATA 
CTTCGACCAC TCAGCATGCT GTACTTCGAG GAGGGAGCAG TCTTGGTTCG ACATCACGAG 
AACATGATCG TAGAGGAGTG CGGTTGCCGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091919.1
CDS	1..1353
Translation	MHRSSDLHLAAVVCLFFVTSVSAFFAPSLHETASSLSDDVVAPGRTRRNTLPLITPDALISVDQHTNSSTMYMVELFESLYTGHNLVNPSKEECQAIEEADTVRNYIGAIQDVKSSSDLVMTWVTSSPTFDQPANEAVHLSELRVELNATSEQSHRFVIQLHQWVNVSCDDGMCQQRKLIHSRDIDARHDDFDGREVFDITKIVKQWIATTKDATSPMEYSIELRAKALIESEADHDKNVLKALLAADIQTEDVLDDIALDSDDVSSEDHGATNQLSVTDVTLVVFSRAPTSPIILSDAHGSVRTRARRSTDEARRRKKQKDLRKLQRDEHKHKMDEKLRLEREQSGPCRRIDMDVDFGRIGWDEWIIYPKQFNAYRCVGTCKGPLDSRDNPSNHAVMQDLVRLHQPERRTPEPCCVPTILRPLSMLYFEEGAVLVRHHENMIVEECGCR

Target ORF information:

RefSeq Version	NM_001098449.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus nodal (LOC579010), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098449.1

ORF Insert Sequence:

ATGCATCGTT CATCGGATCT CCACTTGGCG GCTGTCGTCT GCTTGTTTTT TGTCACATCA 
GTGAGTGCCT TTTTTGCTCC ATCCCTACAT GAGACGGCCT CATCTCTGAG CGACGACGTC 
GTGGCCCCAG GTCGCACCAG ACGGAATACT CTACCTCTTA TCACACCGGA CGCCCTCATC 
AGCGTCGATC AGCATACGAA CTCGTCGACG ATGTACATGG TGGAATTGTT TGAGTCTCTC 
TACACTGGAC ATAATCTTGT CAACCCATCC AAGGAAGAAT GCCAAGCCAT TGAGGAGGCG 
GACACAGTAC GGAACTACAT TGGAGCCATT CAAGATGTCA AATCTTCTTC CGACCTTGTG 
ATGACATGGG TGACCTCTTC CCCGACGTTT GACCAGCCCG CCAACGAAGC TGTCCATCTC 
AGCGAGCTCC GAGTTGAACT CAATGCCACC TCAGAGCAGT CGCATCGTTT CGTCATCCAA 
CTCCACCAGT GGGTCAACGT CTCCTGCGAC GACGGCATGT GTCAGCAACG CAAGCTGATT 
CATTCAAGGG ACATCGACGC TCGCCACGAC GACTTCGATG GCAGGGAGGT GTTCGACATC 
ACCAAGATCG TCAAACAGTG GATCGCAACG ACCAAAGACG CGACGTCCCC AATGGAGTAC 
AGCATCGAGC TACGCGCCAA AGCACTCATC GAATCAGAAG CTGACCACGA CAAGAATGTT 
CTCAAAGCCC TTCTTGCAGC CGACATTCAA ACAGAAGATG TCTTGGATGA TATCGCACTT 
GACTCTGACG ATGTTTCGAG CGAAGACCAC GGAGCTACCA ATCAGCTCTC CGTTACTGAC 
GTGACGCTCG TCGTCTTTTC TCGTGCCCCT ACCTCTCCGA TCATTCTCTC CGATGCTCAC 
GGTTCGGTGC GTACCCGTGC TCGACGATCA ACAGATGAAG CTCGTCGCCG CAAGAAGCAG 
AAAGATCTAC GCAAGCTTCA ACGCGACGAA CACAAGCACA AAATGGACGA GAAGCTTCGC 
TTGGAACGAG AGCAATCTGG GCCTTGTCGT CGAATTGATA TGGACGTTGA TTTCGGTCGA 
ATCGGCTGGG ACGAATGGAT CATCTACCCA AAGCAGTTCA ACGCCTACCG TTGCGTCGGT 
ACGTGTAAGG GACCTCTTGA CAGCCGCGAC AACCCAAGCA ACCACGCAGT CATGCAGGAC 
TTGGTTCGAC TCCACCAACC CGAGAGACGG ACACCTGAAC CTTGCTGCGT ACCAACCATA 
CTTCGACCAC TCAGCATGCT GTACTTCGAG GAGGGAGCAG TCTTGGTTCG ACATCACGAG 
AACATGATCG TAGAGGAGTG CGGTTGCCGG TAA