NFkB cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFkB
Entrez Gene ID	373533
Full Name	NFkB protein
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_030979024.1	XP_030834884.1	NFkB protein isoform X2
XM_011685790.1	XP_011684092.1	NFkB protein isoform X1
NM_214654.1	NP_999819.1	NFkB protein
XM_030979023.1	XP_030834883.1	NFkB protein isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Tu Q, Cameron RA, Worley KC, Gibbs RA, Davidson EH
Genome research22(10)2079-87(2012 Oct)

Origins of immunity: transcription factors and homologues of effector genes of the vertebrate immune system expressed in sea urchin coelomocytes.
Pancer Z, Rast JP, Davidson EH
Immunogenetics49(9)773-86(1999 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NFkB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFkB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd20240	NM_214654.1 Latest version!	Strongylocentrotus purpuratus NFkB protein (NFkB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSd20612	XM_011685790.1 Latest version!	Strongylocentrotus purpuratus NFkB protein (NFkB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSd45308	XM_030979023.1 Latest version!	Strongylocentrotus purpuratus NFkB protein (NFkB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSd45309	XM_030979024.1 Latest version!	Strongylocentrotus purpuratus NFkB protein (NFkB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFkB ( NM_214654.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> NP_999819.1 Strongylocentrotus purpuratus NFkB protein (NFkB), mRNA.

Price & Availability↑

CloneID	OSd20240
Clone ID Related Accession (Same CDS sequence)	NM_214654.1
Accession Version	NM_214654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556812800000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	NFkB protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF064258.1. ##Evidence-Data-START## Transcript exon combination :: AF064258.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01096960, SAMN01103178 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGAGA AATCTGACTC GGTGCAGTCT GTGGAGATTC CAGAGGACAT GTTACAGCAG 
CTCATACACC AGAACGGTCA AGTGGGCTTA CCCATGTCTG GAGCTGAGAA TGATTTCGAT 
AGCAACGACC TGATGCATCT ACCTGTAATG AAAGGTCTTC AGGAAGAGGT GTCCAAACCT 
CATCTCAAGA TATTGGAACA ACCCAGACAG CGAGGATTTC GGTTTCGATA TGGATGTGAG 
GGTCCATCAC ACGGAGGATT ACCAGGCCAG AACAGTCAGA GAGGCAAAAG ATCCTTCCCT 
TCCGTTGAGA TCTGTAATTA CAAAGGCTCC GCCCGTATAG TTGTCTCCTT GGTAACCAAT 
GAAGAGACGC CCCGCCCCCA CGCCCACTCT CTGGTAGGGA AGCACTGTAA GGATGGGCTC 
TGCACAGTCC AGGTTGGCCC CAAGGACATG ACAGCAAGCT TCCCAAACCT GGGCATCCTG 
CACGTGACGC GCAAGGACGT CGTGCCGACC CTCAAGACCC GTATCTTGGC CCAGCATCGT 
CTCTACAAGG ACCTGATCAA CAACTCTACA CCAGGGGAGA GCCACTGGAC GGAGCCGTCC 
GACGCAGAGA TCGAGAAGAA AGCCAAGGAG ATGGCCAAGG ATATGGATCT TAGCGTTGTC 
AGGCTCTGTT TCCAGACCTA TCTGCCGGAC ATCTCAGGAC ACTTCACCCG ACCCCTTGAC 
CCTGTCATAT CAGTGCCAGT CTTTGATAGC AAGGCACCCA ACGCCACGAC CCTCAAGATC 
TGTCGCATGG ATAAGAGTGC AGGATGCTGT ACAGGAGGAG AGGAGGTCTA TCTGCTCTGT 
GATAAGGTCC AGAAGGAGGA CATACAGGTC AAGTTCTTTG AGATCTCTGC CGATGGTCAG 
ATGGTCTGGC AGAGCTTAGC AGAGTTTGGT CCAACCGATG TTCATAGACA GTATGCAATT 
GTATTTAAGA CTCCAGCCTA CAAAGATATA AACATTGATA AACCTGTGTA TGTGCACGTT 
CAGCTCAAGA GGAAATCAGA TAATGAGACC AGTGATCCCA AGCCTTTTAC CTTCCACCCT 
CAAGTACCAG ATCGGGAAGG AATCCTTCGT AAGCGTAAGA AGCACTTGGC TCACTTTAAT 
GAGTATAGTA GCACCTACCA GCAGGGTGGC CTAGGGGGAA GCAATGGCAT GGGAGGAGGA 
GGAGGATCAA CATCTTTTAA TTTCAACAAC CCTAACATGG GGTATGGAGC TAATACCTAC 
AACTTTAGCG GCAACCAGAT GACCTCTTCA ACATCGCAGC CACAATCAGT GCCGCAACAA 
ATCCCTAGCC ACCAGTCTGG GGTCGTTGCT ACCGGCAACT CCTCTCAGCA GATGGTATAC 
ACAACATCGA CCGGTGCACA GAACCACCAG CCCTTGCCTA GCATTGCAAC ACTAGCAAGG 
AAACCCCAAG AGCAGTTACA GCAACAACAA TTCCAGTTGC AGCAACAACT GCAGCAGCAA 
CAGCAGCAAC AACAGCAGCA ACAGCAGCAA CAGCAACGAC AGCAGCAACA GCAGCAGCCG 
ATGCAGATGG ATTTCCAGAT GCCACCTATC CAAGGCAGCG CGATGGGAGG CCAGGCTGGT 
GGAGATCGCG TTAAGATGGA GAGTGAGAGC AAATACTGTG GCAACATGGA TACAGCAGAA 
GGATTTCAGC TGGACTCCAA TACATTACGT GATTGTGGGC CACTACCAGA CCTCAAAGAA 
CTTCAAGATA CAGGTGTCTT GGATCTCATC CCAAAGGGTA TCTCAAGACC CAAGGATATG 
GTGGAGAAAA ATATTCAAAC TGATGACATA ACAATAGACA GTGATTCCAT GGCGTGGCAT 
GTTGCCCAGG TAACAGCCAA CGCTCTTCAT GACTACGCAG CTACGGGTGA CATTAAAACA 
ATCCTAACGG TCCAGAGACA TCTTATCGCT GTGGAAGATG ATAACGGAGA TACAGCGCTG 
CATGCTGCAA TCATCAACAA GAAGTATGAT GTGACACATG CGCTTCTCTC GGCTGTAATC 
AAGATTCCAG ATCAGATCAT CGTTAACCAG ACTAACCATT TGAAACAGAC TCCCCTCCAC 
CTGGCCGTCA TCACAAATCA ATCCAAGATG GTGGAGGTCC TTTTGAGATG CGGGGCCAAC 
CCCAACCTCT GTGATCATGA GGGTAATACC CCCCTCCATC TGGCCACTAT GATGGGTATG 
ACAGAAGGTG TAAACTTCCT GGTGCGTGGC CCCAAGGCTA AGGCAGCCAT TAAACCCATC 
AAGACCGACA TTAATCCAAC AAACTATGAA GGCCTTGCCC CAGTCCACCT GGCTGTGATC 
GCTAAGAACT TGGATATCCT TAAGGCACTT GTGTCGTCAG GAGCGGATGT CAATGTAGCT 
GATGGGAAGA CCGGCCGAAC CGCCCTCCAC TATGCCGTGG AAGTCGAGAG CTTCCCAATT 
CTGGGCTACC TCCTGATCGA AGCCAAGGTC GACATCAATG CCGTGACCTT CTGTGGGGAC 
TCGGCCCTTC ACCTGGCCAG TAGCCTGGAT CTGAGGGCGG TGGCCACGCT CCTCATCGCA 
GCAGGAGCTG ACCCTAAGCT GGAGAACGCC GACCTGGGGG ACGACAGTGA CAGCGATGTC 
GACGGGGAGG ATGACGATGG TGAGAAGGAT GGGGAGAAGA AGGAGGAGGG AGTGGAAGAG 
GAGGAGAAGG AGAAGCATGG GAAGACACCT TATCAGCTGG CCACAAGTGC TAAGATGAAA 
GAAGTCTTGT CTGGACATTC CACCAGGAGA GTAGAGGAGG AAGATTTTGA CTACATCGAG 
ACCGGAAGCG CCATCAGTTC CGTTGGGTCA ATCCAATCAG AGGACCACAT GAAAGTCAAT 
AGCTGGGACC AGGGCTACTT CTCAGGGAAG TCCAGCTCCT CTGTCTCTAA CCTGGAACTG 
AAGCGAACCT GGCCCAGAGT TAAAAGCCGA ATGCTAGGGA GCTCCCTCGT CACATCTAAA 
GGAGACATTG GCCTGTTGTC TGAAACCACC ACCGAGAAGC TAGGCTCTAT GTTGGATGAT 
AATTACCCTA CTACACAGAG CTGGTTTACC CTGGCCAATC GGCTCGGACT CTCCAACATG 
CTCAACTTTC TCAAGCTTGT CCCAAGCCCC ACTGCAGTCA TTCTCAAACA GTTTGAGGCT 
ATGGATGGAA CCATCAAGGA GCTCCGAGAC GTCCTCTCAT CAATGAATCA CATAGAAGCT 
GTGGCCTTAC TGGATCAGGC TGTCTCATGG AGGAAAGAGA AAGCTGATGA GGCTCATATG 
TACAGTTCGA TGCACCAGTC TTTGGGTAAG ATGAATTTGT CCGACGCTCA CATGCATCAA 
CATCAATTCG TTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999819.1
CDS	398..3775
Translation	MEEKSDSVQSVEIPEDMLQQLIHQNGQVGLPMSGAENDFDSNDLMHLPVMKGLQEEVSKPHLKILEQPRQRGFRFRYGCEGPSHGGLPGQNSQRGKRSFPSVEICNYKGSARIVVSLVTNEETPRPHAHSLVGKHCKDGLCTVQVGPKDMTASFPNLGILHVTRKDVVPTLKTRILAQHRLYKDLINNSTPGESHWTEPSDAEIEKKAKEMAKDMDLSVVRLCFQTYLPDISGHFTRPLDPVISVPVFDSKAPNATTLKICRMDKSAGCCTGGEEVYLLCDKVQKEDIQVKFFEISADGQMVWQSLAEFGPTDVHRQYAIVFKTPAYKDINIDKPVYVHVQLKRKSDNETSDPKPFTFHPQVPDREGILRKRKKHLAHFNEYSSTYQQGGLGGSNGMGGGGGSTSFNFNNPNMGYGANTYNFSGNQMTSSTSQPQSVPQQIPSHQSGVVATGNSSQQMVYTTSTGAQNHQPLPSIATLARKPQEQLQQQQFQLQQQLQQQQQQQQQQQQQQQRQQQQQQPMQMDFQMPPIQGSAMGGQAGGDRVKMESESKYCGNMDTAEGFQLDSNTLRDCGPLPDLKELQDTGVLDLIPKGISRPKDMVEKNIQTDDITIDSDSMAWHVAQVTANALHDYAATGDIKTILTVQRHLIAVEDDNGDTALHAAIINKKYDVTHALLSAVIKIPDQIIVNQTNHLKQTPLHLAVITNQSKMVEVLLRCGANPNLCDHEGNTPLHLATMMGMTEGVNFLVRGPKAKAAIKPIKTDINPTNYEGLAPVHLAVIAKNLDILKALVSSGADVNVADGKTGRTALHYAVEVESFPILGYLLIEAKVDINAVTFCGDSALHLASSLDLRAVATLLIAAGADPKLENADLGDDSDSDVDGEDDDGEKDGEKKEEGVEEEEKEKHGKTPYQLATSAKMKEVLSGHSTRRVEEEDFDYIETGSAISSVGSIQSEDHMKVNSWDQGYFSGKSSSSVSNLELKRTWPRVKSRMLGSSLVTSKGDIGLLSETTTEKLGSMLDDNYPTTQSWFTLANRLGLSNMLNFLKLVPSPTAVILKQFEAMDGTIKELRDVLSSMNHIEAVALLDQAVSWRKEKADEAHMYSSMHQSLGKMNLSDAHMHQHQFVY

Target ORF information:

RefSeq Version	NM_214654.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus NFkB protein (NFkB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214654.1

ORF Insert Sequence:

ATGGAGGAGA AATCTGACTC GGTGCAGTCT GTGGAGATTC CAGAGGACAT GTTACAGCAG 
CTCATACACC AGAACGGTCA AGTGGGCTTA CCCATGTCTG GAGCTGAGAA TGATTTCGAT 
AGCAACGACC TGATGCATCT ACCTGTAATG AAAGGTCTTC AGGAAGAGGT GTCCAAACCT 
CATCTCAAGA TATTGGAACA ACCCAGACAG CGAGGATTTC GGTTTCGATA TGGATGTGAG 
GGTCCATCAC ACGGAGGATT ACCAGGCCAG AACAGTCAGA GAGGCAAAAG ATCCTTCCCT 
TCCGTTGAGA TCTGTAATTA CAAAGGCTCC GCCCGTATAG TTGTCTCCTT GGTAACCAAT 
GAAGAGACGC CCCGCCCCCA CGCCCACTCT CTGGTAGGGA AGCACTGTAA GGATGGGCTC 
TGCACAGTCC AGGTTGGCCC CAAGGACATG ACAGCAAGCT TCCCAAACCT GGGCATCCTG 
CACGTGACGC GCAAGGACGT CGTGCCGACC CTCAAGACCC GTATCTTGGC CCAGCATCGT 
CTCTACAAGG ACCTGATCAA CAACTCTACA CCAGGGGAGA GCCACTGGAC GGAGCCGTCC 
GACGCAGAGA TCGAGAAGAA AGCCAAGGAG ATGGCCAAGG ATATGGATCT TAGCGTTGTC 
AGGCTCTGTT TCCAGACCTA TCTGCCGGAC ATCTCAGGAC ACTTCACCCG ACCCCTTGAC 
CCTGTCATAT CAGTGCCAGT CTTTGATAGC AAGGCACCCA ACGCCACGAC CCTCAAGATC 
TGTCGCATGG ATAAGAGTGC AGGATGCTGT ACAGGAGGAG AGGAGGTCTA TCTGCTCTGT 
GATAAGGTCC AGAAGGAGGA CATACAGGTC AAGTTCTTTG AGATCTCTGC CGATGGTCAG 
ATGGTCTGGC AGAGCTTAGC AGAGTTTGGT CCAACCGATG TTCATAGACA GTATGCAATT 
GTATTTAAGA CTCCAGCCTA CAAAGATATA AACATTGATA AACCTGTGTA TGTGCACGTT 
CAGCTCAAGA GGAAATCAGA TAATGAGACC AGTGATCCCA AGCCTTTTAC CTTCCACCCT 
CAAGTACCAG ATCGGGAAGG AATCCTTCGT AAGCGTAAGA AGCACTTGGC TCACTTTAAT 
GAGTATAGTA GCACCTACCA GCAGGGTGGC CTAGGGGGAA GCAATGGCAT GGGAGGAGGA 
GGAGGATCAA CATCTTTTAA TTTCAACAAC CCTAACATGG GGTATGGAGC TAATACCTAC 
AACTTTAGCG GCAACCAGAT GACCTCTTCA ACATCGCAGC CACAATCAGT GCCGCAACAA 
ATCCCTAGCC ACCAGTCTGG GGTCGTTGCT ACCGGCAACT CCTCTCAGCA GATGGTATAC 
ACAACATCGA CCGGTGCACA GAACCACCAG CCCTTGCCTA GCATTGCAAC ACTAGCAAGG 
AAACCCCAAG AGCAGTTACA GCAACAACAA TTCCAGTTGC AGCAACAACT GCAGCAGCAA 
CAGCAGCAAC AACAGCAGCA ACAGCAGCAA CAGCAACGAC AGCAGCAACA GCAGCAGCCG 
ATGCAGATGG ATTTCCAGAT GCCACCTATC CAAGGCAGCG CGATGGGAGG CCAGGCTGGT 
GGAGATCGCG TTAAGATGGA GAGTGAGAGC AAATACTGTG GCAACATGGA TACAGCAGAA 
GGATTTCAGC TGGACTCCAA TACATTACGT GATTGTGGGC CACTACCAGA CCTCAAAGAA 
CTTCAAGATA CAGGTGTCTT GGATCTCATC CCAAAGGGTA TCTCAAGACC CAAGGATATG 
GTGGAGAAAA ATATTCAAAC TGATGACATA ACAATAGACA GTGATTCCAT GGCGTGGCAT 
GTTGCCCAGG TAACAGCCAA CGCTCTTCAT GACTACGCAG CTACGGGTGA CATTAAAACA 
ATCCTAACGG TCCAGAGACA TCTTATCGCT GTGGAAGATG ATAACGGAGA TACAGCGCTG 
CATGCTGCAA TCATCAACAA GAAGTATGAT GTGACACATG CGCTTCTCTC GGCTGTAATC 
AAGATTCCAG ATCAGATCAT CGTTAACCAG ACTAACCATT TGAAACAGAC TCCCCTCCAC 
CTGGCCGTCA TCACAAATCA ATCCAAGATG GTGGAGGTCC TTTTGAGATG CGGGGCCAAC 
CCCAACCTCT GTGATCATGA GGGTAATACC CCCCTCCATC TGGCCACTAT GATGGGTATG 
ACAGAAGGTG TAAACTTCCT GGTGCGTGGC CCCAAGGCTA AGGCAGCCAT TAAACCCATC 
AAGACCGACA TTAATCCAAC AAACTATGAA GGCCTTGCCC CAGTCCACCT GGCTGTGATC 
GCTAAGAACT TGGATATCCT TAAGGCACTT GTGTCGTCAG GAGCGGATGT CAATGTAGCT 
GATGGGAAGA CCGGCCGAAC CGCCCTCCAC TATGCCGTGG AAGTCGAGAG CTTCCCAATT 
CTGGGCTACC TCCTGATCGA AGCCAAGGTC GACATCAATG CCGTGACCTT CTGTGGGGAC 
TCGGCCCTTC ACCTGGCCAG TAGCCTGGAT CTGAGGGCGG TGGCCACGCT CCTCATCGCA 
GCAGGAGCTG ACCCTAAGCT GGAGAACGCC GACCTGGGGG ACGACAGTGA CAGCGATGTC 
GACGGGGAGG ATGACGATGG TGAGAAGGAT GGGGAGAAGA AGGAGGAGGG AGTGGAAGAG 
GAGGAGAAGG AGAAGCATGG GAAGACACCT TATCAGCTGG CCACAAGTGC TAAGATGAAA 
GAAGTCTTGT CTGGACATTC CACCAGGAGA GTAGAGGAGG AAGATTTTGA CTACATCGAG 
ACCGGAAGCG CCATCAGTTC CGTTGGGTCA ATCCAATCAG AGGACCACAT GAAAGTCAAT 
AGCTGGGACC AGGGCTACTT CTCAGGGAAG TCCAGCTCCT CTGTCTCTAA CCTGGAACTG 
AAGCGAACCT GGCCCAGAGT TAAAAGCCGA ATGCTAGGGA GCTCCCTCGT CACATCTAAA 
GGAGACATTG GCCTGTTGTC TGAAACCACC ACCGAGAAGC TAGGCTCTAT GTTGGATGAT 
AATTACCCTA CTACACAGAG CTGGTTTACC CTGGCCAATC GGCTCGGACT CTCCAACATG 
CTCAACTTTC TCAAGCTTGT CCCAAGCCCC ACTGCAGTCA TTCTCAAACA GTTTGAGGCT 
ATGGATGGAA CCATCAAGGA GCTCCGAGAC GTCCTCTCAT CAATGAATCA CATAGAAGCT 
GTGGCCTTAC TGGATCAGGC TGTCTCATGG AGGAAAGAGA AAGCTGATGA GGCTCATATG 
TACAGTTCGA TGCACCAGTC TTTGGGTAAG ATGAATTTGT CCGACGCTCA CATGCATCAA 
CATCAATTCG TTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFkB ( XM_011685790.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011684092.1 Strongylocentrotus purpuratus NFkB protein (NFkB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd20612
Clone ID Related Accession (Same CDS sequence)	XM_011685790.1
Accession Version	XM_011685790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	NFkB protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011996344.1) and transcript sequence (AF064258.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 115 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGATGGAGG AGAAATCTGA CTCGGTGCAG TCTGTGGAGA TTCCAGAGGA CATGTTACAG 
CAGCTCATAC ACCAGAACGG TCAAGTGGGC TTACCCATGT CTGGAGCTGA GAATGATTTC 
GATAGCAACG ACCTGATGCA TCTACCTGTA ATGAAAGGTC TTCAGGAAGA GGTGTCCAAA 
CCTCATCTCA AGATATTGGA ACAACCCAGA CAGCGAGGAT TTCGGTTTCG ATATGGATGT 
GAGGGTCCAT CACACGGAGG ATTACCAGGT CAGAACAGTC AGAGAGGCAA AAGATCCTTC 
CCTTCCGTTG AGATCTGTAA TTACAAAGGC TCTGCCCGTA TAGTTGTCTC CTTGGTAACC 
AATGAAGAGA CGCCCCGCCC CCACGCCCAC TCTCTGGTAG GGAAGCACTG TAAGGATGGG 
CTCTGCACAG TCCAGGTCGG CCCCAAGGAT ATGACAGCAA GCTTCCCAAA CCTGGGCATC 
CTGCACGTGA CGCGCAAGGA CGTCGTCCCG ACCCTCAAGA CCCGTATCTT GGCCCAGCAT 
CGTCTCTACA AGGATCTGAT CAACAACTCT ACACCAGGGG AAAGCCACTG GACGGAGCCG 
TCCGATGCAG AGATCGAGAA GAAAGCCAAG GAGATGGCTA AGGATATGGA TCTTAGCGTC 
GTCCGGCTCT GCTTCCAGAC CTATCTGCCG GACATCTCAG GACACTTCAC CCGACCCCTT 
GACCCTGTCA TATCAGTGCC AGTCTTTGAT AGCAAGGCAC CCAACGCCAC GACCCTCAAG 
ATCTGTCGCA TGGACAAGAG TGCAGGATGC TGTACAGGAG GAGAGGAGGT CTATCTGCTC 
TGTGATAAGG TCCAGAAGGA GGACATACAG GTCAAGTTCT TTGAGATCTC TGCCGATGGT 
CAGATGGTCT GGCAGAGCTT AGCAGAGTTT GGTCCAACCG ATGTTCATAG ACAGTATGCA 
ATTGTATTTA AGACTCCAGC CTACAAAGAT ATAAACATTG ATAAACCTGT GTATGTGCAC 
GTTCAGCTCA AGAGGAAATC AGATAATGAG ACCAGCGATC CCAAGCCTTT CACCTTCCAC 
CCTCAAGTAC CAGATCGGGA AGGAATCCTT CGTAAGCGTA AGAAGCACTT GGCTCACTTT 
AATGAGTATA GTAGCACCTA CCAGCAGGGT GGCCTAGGGG GAAGCAGTGG CATGGGAGGA 
GGAGGAGGAT CAACATCTTT TAATTTCAAC AACCCTAACA TGGGGTATGG AGCTAATACC 
TACAACTTTA GCGGCAACCA AATGACCTCT TCAACATCGC AGCCACAATC AGTGCCGCAA 
CAAATCCCTA GCCACCAGTC TGGGGTCGTT GCTACCGGCA ACTCCTCTCA GCAGATGGTA 
TACACAACAT CGACCGGTGC ACAGAACCAC CAGCCCTTGC CTAGCATTGC AACACTAGCA 
AGGAAACCCC AAGAGCAGCC ACAGCAACAA CAATTCCAGT TGCAGCAACA ACTGCAGCAG 
CAACAGCAGC AACAACAGCA GCGACAGCAG CAACAGCAGC GACAGCAGCA ACAGCAGCAG 
CCGATGCAGA TGGATTTCCA GATGCCACCT ATCCAAGGCA GTGCGATGGG AGGCCAGGCT 
GGTGGAGATC GCGTTAAGAT GGAGAGTGAG AGCAAATACT GTGGCAACAT GGATACAGCA 
GAAGGATTTC AGCTGGACTC CAATACATTA CGTGATTGTG GGCCGCTACC AGACCTCAAA 
GAACTTCAAG ATACAGGTGT CTTGGATCTC ATCCCAAAGG GTATCTCAAG ACCAAAGGAT 
ATGGTGGAGA AAAATATTCA AACTGATGAC ATAACAATAG ACAGTGATTC CATGGCATGG 
CATGTTGCCC AGGTAACAGC CAATGCTCTT CATGACTACG CAGCTACGGG TGACATTAAA 
ACAATCCTAA CGGTCCAGAG ACATCTTATC GCTGTGGAAG ATGATAACGG AGATACAGCG 
CTGCATGCTG CAATCATCAA CAAGAAGTAT GATGTGACAC ATGCGCTTCT CTCGGCTGTA 
ATCAAGATTC CAGATCAGAT CATCGTTAAC CAGACTAACC ATTTGAAACA GACTCCCCTC 
CACCTGGCCG TCATCACAAA TCAATCCAAG ATGGTGGAGG TCCTTTTGAG ATGCGGGGCC 
AACCCCAACC TCTGTGATCA TGAGGGTAAT ACCCCCCTCC ATCTGGCCAC TAGGATGGGT 
ATGGCAGAAG GTGTAAACTT CCTGGTGCGT GGCCCCAAGG CTAAGGCAGC CATTAAACCC 
ATCAAGACCG ACATTAATCC AACAAACTAT GAAGGCCTTG CCCCAGTCCA CCTGGCTGTG 
ATCGCTAAGA ACTTGGATAT CCTTAAGGCA CTTGTGTCAT CAGGAGCGGA TGTCAACGTA 
GCTGATGGGA AGACCGGCCG AACCGCCCTC CACTATGCCG TGGAAGTCGA GAGCTTCCCA 
ATTCTGGGCT ACCTCCTGAT CGAAGCCAAG GTCGACATCA ATGCCGTGAC CTTCTGTGGG 
GACTCGGCCC TTCACCTGGC CAGTAGCCTG GATCTGAGGG CGGTGGCCAC GCTCCTCATC 
GCAGCAGGAG CTGACCCTAA GCTGGAGAAC GCCGACCTGG GGGACGACAG TGACAGCGAT 
GTCGACGGGG AGGATGACGA TGGTGAGAAG GATGAGGAGA AGAAGGAGGA GGGAGTGGAA 
GAAGAGGAGA AGGAGAAGCA TGGGAAGACA CCCTATCAGC TGGCCACAAG TGCTAAGATG 
AAAGAAGTCT TGTCTGGACA TTCCACCAGG AGAGTAGAGG AGGAGGATTT TGACTACATC 
GAGACAGGAA GTGCCATCAG TTCCGTTGGG TCAATCCAAT CAGAGGACCA CATGAAAGTC 
AATAGCTGGG ACCAGGGCTA CTTCTCAGGG AAGTCCAGCT CCTCTGTCTC TAACCTGGAA 
CTAAAGCGAA CCTGGCCCAG AGTTAAAAGC AGAATGCTAG GGAGCTCCCT CATCACATCT 
AAAGGAGACA TTGGCCTGTT GTCTGAAACC ACCACCGAGA AGCTAGGCTC TATGTTGGAT 
GATAATTACC CTACTACACA GAGCTGGTTT ACCCTGGCCA ATCGGCTCGG ACTCTCCAAC 
ATGCTCAACT TTCTCAAGCT TGTCCCAAGT CCCACTGCAG TCATTCTCAA ACAGTTTGAG 
GCTATGGATG GAACCATTAA GGAGCTCCGA GACGTCCTCT ATTCAATGAA TCACATAGAA 
GCTGTGGCCT TACTGGATCA GGCCGTCTCA TGGAGGAAAG AGAAAGCTGA TGAGGCTCAT 
ATGTACAGTT CGATGCACCA GTCTTTGGGT AAGATGAATT TGTCCGACGC TCACATGCAT 
CAACATCAAT TCGTTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011684092.1
CDS	191..3571
Translation	MMEEKSDSVQSVEIPEDMLQQLIHQNGQVGLPMSGAENDFDSNDLMHLPVMKGLQEEVSKPHLKILEQPRQRGFRFRYGCEGPSHGGLPGQNSQRGKRSFPSVEICNYKGSARIVVSLVTNEETPRPHAHSLVGKHCKDGLCTVQVGPKDMTASFPNLGILHVTRKDVVPTLKTRILAQHRLYKDLINNSTPGESHWTEPSDAEIEKKAKEMAKDMDLSVVRLCFQTYLPDISGHFTRPLDPVISVPVFDSKAPNATTLKICRMDKSAGCCTGGEEVYLLCDKVQKEDIQVKFFEISADGQMVWQSLAEFGPTDVHRQYAIVFKTPAYKDINIDKPVYVHVQLKRKSDNETSDPKPFTFHPQVPDREGILRKRKKHLAHFNEYSSTYQQGGLGGSSGMGGGGGSTSFNFNNPNMGYGANTYNFSGNQMTSSTSQPQSVPQQIPSHQSGVVATGNSSQQMVYTTSTGAQNHQPLPSIATLARKPQEQPQQQQFQLQQQLQQQQQQQQQRQQQQQRQQQQQQPMQMDFQMPPIQGSAMGGQAGGDRVKMESESKYCGNMDTAEGFQLDSNTLRDCGPLPDLKELQDTGVLDLIPKGISRPKDMVEKNIQTDDITIDSDSMAWHVAQVTANALHDYAATGDIKTILTVQRHLIAVEDDNGDTALHAAIINKKYDVTHALLSAVIKIPDQIIVNQTNHLKQTPLHLAVITNQSKMVEVLLRCGANPNLCDHEGNTPLHLATRMGMAEGVNFLVRGPKAKAAIKPIKTDINPTNYEGLAPVHLAVIAKNLDILKALVSSGADVNVADGKTGRTALHYAVEVESFPILGYLLIEAKVDINAVTFCGDSALHLASSLDLRAVATLLIAAGADPKLENADLGDDSDSDVDGEDDDGEKDEEKKEEGVEEEEKEKHGKTPYQLATSAKMKEVLSGHSTRRVEEEDFDYIETGSAISSVGSIQSEDHMKVNSWDQGYFSGKSSSSVSNLELKRTWPRVKSRMLGSSLITSKGDIGLLSETTTEKLGSMLDDNYPTTQSWFTLANRLGLSNMLNFLKLVPSPTAVILKQFEAMDGTIKELRDVLYSMNHIEAVALLDQAVSWRKEKADEAHMYSSMHQSLGKMNLSDAHMHQHQFVY

Target ORF information:

RefSeq Version	XM_011685790.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus NFkB protein (NFkB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011685790.1

ORF Insert Sequence:

ATGATGGAGG AGAAATCTGA CTCGGTGCAG TCTGTGGAGA TTCCAGAGGA CATGTTACAG 
CAGCTCATAC ACCAGAACGG TCAAGTGGGC TTACCCATGT CTGGAGCTGA GAATGATTTC 
GATAGCAACG ACCTGATGCA TCTACCTGTA ATGAAAGGTC TTCAGGAAGA GGTGTCCAAA 
CCTCATCTCA AGATATTGGA ACAACCCAGA CAGCGAGGAT TTCGGTTTCG ATATGGATGT 
GAGGGTCCAT CACACGGAGG ATTACCAGGT CAGAACAGTC AGAGAGGCAA AAGATCCTTC 
CCTTCCGTTG AGATCTGTAA TTACAAAGGC TCTGCCCGTA TAGTTGTCTC CTTGGTAACC 
AATGAAGAGA CGCCCCGCCC CCACGCCCAC TCTCTGGTAG GGAAGCACTG TAAGGATGGG 
CTCTGCACAG TCCAGGTCGG CCCCAAGGAT ATGACAGCAA GCTTCCCAAA CCTGGGCATC 
CTGCACGTGA CGCGCAAGGA CGTCGTCCCG ACCCTCAAGA CCCGTATCTT GGCCCAGCAT 
CGTCTCTACA AGGATCTGAT CAACAACTCT ACACCAGGGG AAAGCCACTG GACGGAGCCG 
TCCGATGCAG AGATCGAGAA GAAAGCCAAG GAGATGGCTA AGGATATGGA TCTTAGCGTC 
GTCCGGCTCT GCTTCCAGAC CTATCTGCCG GACATCTCAG GACACTTCAC CCGACCCCTT 
GACCCTGTCA TATCAGTGCC AGTCTTTGAT AGCAAGGCAC CCAACGCCAC GACCCTCAAG 
ATCTGTCGCA TGGACAAGAG TGCAGGATGC TGTACAGGAG GAGAGGAGGT CTATCTGCTC 
TGTGATAAGG TCCAGAAGGA GGACATACAG GTCAAGTTCT TTGAGATCTC TGCCGATGGT 
CAGATGGTCT GGCAGAGCTT AGCAGAGTTT GGTCCAACCG ATGTTCATAG ACAGTATGCA 
ATTGTATTTA AGACTCCAGC CTACAAAGAT ATAAACATTG ATAAACCTGT GTATGTGCAC 
GTTCAGCTCA AGAGGAAATC AGATAATGAG ACCAGCGATC CCAAGCCTTT CACCTTCCAC 
CCTCAAGTAC CAGATCGGGA AGGAATCCTT CGTAAGCGTA AGAAGCACTT GGCTCACTTT 
AATGAGTATA GTAGCACCTA CCAGCAGGGT GGCCTAGGGG GAAGCAGTGG CATGGGAGGA 
GGAGGAGGAT CAACATCTTT TAATTTCAAC AACCCTAACA TGGGGTATGG AGCTAATACC 
TACAACTTTA GCGGCAACCA AATGACCTCT TCAACATCGC AGCCACAATC AGTGCCGCAA 
CAAATCCCTA GCCACCAGTC TGGGGTCGTT GCTACCGGCA ACTCCTCTCA GCAGATGGTA 
TACACAACAT CGACCGGTGC ACAGAACCAC CAGCCCTTGC CTAGCATTGC AACACTAGCA 
AGGAAACCCC AAGAGCAGCC ACAGCAACAA CAATTCCAGT TGCAGCAACA ACTGCAGCAG 
CAACAGCAGC AACAACAGCA GCGACAGCAG CAACAGCAGC GACAGCAGCA ACAGCAGCAG 
CCGATGCAGA TGGATTTCCA GATGCCACCT ATCCAAGGCA GTGCGATGGG AGGCCAGGCT 
GGTGGAGATC GCGTTAAGAT GGAGAGTGAG AGCAAATACT GTGGCAACAT GGATACAGCA 
GAAGGATTTC AGCTGGACTC CAATACATTA CGTGATTGTG GGCCGCTACC AGACCTCAAA 
GAACTTCAAG ATACAGGTGT CTTGGATCTC ATCCCAAAGG GTATCTCAAG ACCAAAGGAT 
ATGGTGGAGA AAAATATTCA AACTGATGAC ATAACAATAG ACAGTGATTC CATGGCATGG 
CATGTTGCCC AGGTAACAGC CAATGCTCTT CATGACTACG CAGCTACGGG TGACATTAAA 
ACAATCCTAA CGGTCCAGAG ACATCTTATC GCTGTGGAAG ATGATAACGG AGATACAGCG 
CTGCATGCTG CAATCATCAA CAAGAAGTAT GATGTGACAC ATGCGCTTCT CTCGGCTGTA 
ATCAAGATTC CAGATCAGAT CATCGTTAAC CAGACTAACC ATTTGAAACA GACTCCCCTC 
CACCTGGCCG TCATCACAAA TCAATCCAAG ATGGTGGAGG TCCTTTTGAG ATGCGGGGCC 
AACCCCAACC TCTGTGATCA TGAGGGTAAT ACCCCCCTCC ATCTGGCCAC TAGGATGGGT 
ATGGCAGAAG GTGTAAACTT CCTGGTGCGT GGCCCCAAGG CTAAGGCAGC CATTAAACCC 
ATCAAGACCG ACATTAATCC AACAAACTAT GAAGGCCTTG CCCCAGTCCA CCTGGCTGTG 
ATCGCTAAGA ACTTGGATAT CCTTAAGGCA CTTGTGTCAT CAGGAGCGGA TGTCAACGTA 
GCTGATGGGA AGACCGGCCG AACCGCCCTC CACTATGCCG TGGAAGTCGA GAGCTTCCCA 
ATTCTGGGCT ACCTCCTGAT CGAAGCCAAG GTCGACATCA ATGCCGTGAC CTTCTGTGGG 
GACTCGGCCC TTCACCTGGC CAGTAGCCTG GATCTGAGGG CGGTGGCCAC GCTCCTCATC 
GCAGCAGGAG CTGACCCTAA GCTGGAGAAC GCCGACCTGG GGGACGACAG TGACAGCGAT 
GTCGACGGGG AGGATGACGA TGGTGAGAAG GATGAGGAGA AGAAGGAGGA GGGAGTGGAA 
GAAGAGGAGA AGGAGAAGCA TGGGAAGACA CCCTATCAGC TGGCCACAAG TGCTAAGATG 
AAAGAAGTCT TGTCTGGACA TTCCACCAGG AGAGTAGAGG AGGAGGATTT TGACTACATC 
GAGACAGGAA GTGCCATCAG TTCCGTTGGG TCAATCCAAT CAGAGGACCA CATGAAAGTC 
AATAGCTGGG ACCAGGGCTA CTTCTCAGGG AAGTCCAGCT CCTCTGTCTC TAACCTGGAA 
CTAAAGCGAA CCTGGCCCAG AGTTAAAAGC AGAATGCTAG GGAGCTCCCT CATCACATCT 
AAAGGAGACA TTGGCCTGTT GTCTGAAACC ACCACCGAGA AGCTAGGCTC TATGTTGGAT 
GATAATTACC CTACTACACA GAGCTGGTTT ACCCTGGCCA ATCGGCTCGG ACTCTCCAAC 
ATGCTCAACT TTCTCAAGCT TGTCCCAAGT CCCACTGCAG TCATTCTCAA ACAGTTTGAG 
GCTATGGATG GAACCATTAA GGAGCTCCGA GACGTCCTCT ATTCAATGAA TCACATAGAA 
GCTGTGGCCT TACTGGATCA GGCCGTCTCA TGGAGGAAAG AGAAAGCTGA TGAGGCTCAT 
ATGTACAGTT CGATGCACCA GTCTTTGGGT AAGATGAATT TGTCCGACGC TCACATGCAT 
CAACATCAAT TCGTTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFkB ( XM_030979023.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030834883.1 Strongylocentrotus purpuratus NFkB protein (NFkB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd45308
Clone ID Related Accession (Same CDS sequence)	XM_030979023.1
Accession Version	XM_030979023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	NFkB protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145595.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGG AGAAATCTGA CTCGGTGCAG TCTGTGGAGA TTCCAGAGGA CATGTTACAG 
CAGCTCATAC ACCAGAACGG TCAAGTGGGC TTACCCATGT CTGGAGCTGA GAATGATTTC 
GATAGCAACG ACCTGATGCA TCTACCTGTA ATGAAAGGTC TTCAGGAAGA GGTGTCCAAA 
CCTCATCTCA AGATATTGGA ACAACCCAGA CAGCGAGGAT TTCGGTTTCG ATATGGATGT 
GAGGGTCCAT CACACGGAGG ATTACCAGGC CAGAACAGTC AGAGAGGCAA AAGATCCTTC 
CCTTCCGTTG AGATCTGTAA TTACAAAGGC TCTGCCCGTA TAGTTGTCTC CTTGGTAACC 
AATGAAGAGA CGCCCCGCCC CCACGCCCAC TCTCTGGTAG GGAAGCACTG TAAGGATGGG 
CTCTGCACAG TCCAGGTCGG CCCCAAGGAT ATGACAGCAA GGTCAGAAAT GAGCTTCCCA 
AACCTGGGCA TCCTGCACGT GACGCGCAAG GACGTCGTCC CGACCCTCAA GACCCGTATC 
TTGGCCCAGC ATCGTCTCTA CAAGGATCTG ATCAACAACT CTACACCAGG GGAAAGCCAC 
TGGACGGAGC CGTCCGATGC AGAGATCGAG AAGAAAGCCA AGGAGATGGC TAAGGATATG 
GATCTTAGCG TCGTCCGGCT CTGCTTCCAG ACCTATCTGC CGGACATCTC AGGACACTTC 
ACCCGACCCC TTGACCCTGT CATATCAGTG CCAGTCTTTG ATAGCAAGGC ACCCAACGCC 
ACGACCCTCA AGATCTGTCG CATGGACAAG AGTGCAGGAT GCTGTACAGG AGGAGAGGAG 
GTCTATCTGC TCTGTGATAA GGTCCAGAAG GAGGACATAC AGGTCAAGTT CTTTGAGATC 
TCTGCCGATG GTCAGATGGT CTGGCAGAGC TTAGCAGAGT TTGGTCCAAC CGATGTTCAT 
AGACAGTATG CAATTGTATT TAAGACTCCA GCCTACAAAG ATATAAACAT TGATAAACCT 
GTGTATGTGC ACGTTCAGCT CAAGAGGAAA TCAGATAATG AGACCAGCGA TCCCAAGCCT 
TTCACCTTCC ACCCTCAAGT ACCAGATCGG GAAGGAATCC TTCGTAAGCG TAAGAAGCAC 
TTGGCTCACT TTAATGAGTA TAGTAGCACC TACCAGCAGG GTGGCCTAGG GGGAAGCAGT 
GGCATGGGAG GAGGAGGAGG ATCAACATCT TTTAATTTCA ACAACCCTAA CATGGGGTAT 
GGAGCTAATA CCTACAACTT TAGCGGCAAC CAAATGACCT CTTCAACATC GCAGCCACAA 
TCAGTGCCGC AACAAATCCC TAGCCACCAG TCTGGGGTCG TTGCTACCGG CAACTCCTCT 
CAGCAGATGG TATACACAAC ATCGACCGGT GCACAGAACC ACCAGCCCTT GCCTAGCATT 
GCAACACTAG CAAGGAAACC CCAAGAGCAG CCACAGCAAC AACAATTCCA GTTGCAGCAA 
CAACTGCAGC AGCAACAGCA GCAACAACAG CAGCGACAGC AGCAACAGCA GCGACAGCAG 
CAACAGCAGC AGCCGATGCA GATGGATTTC CAGATGCCAC CTATCCAAGG CAGTGCGATG 
GGAGGCCAGG CTGGTGGAGA TCGCGTTAAG ATGGAGAGTG AGAGCAAATA CTGTGGCAAC 
ATGGATACAG CAGAAGGATT TCAGCTGGAC TCCAATACAT TACGTGATTG TGGGCCGCTA 
CCAGACCTCA AAGAACTTCA AGATACAGGT GTCTTGGATC TCATCCCAAA GGGTATCTCA 
AGACCAAAGG ATATGGTGGA GAAAAATATT CAAACTGATG ACATAACAAT AGACAGTGAT 
TCCATGGCAT GGCATGTTGC CCAGGTAACA GCCAATGCTC TTCATGACTA CGCAGCTACG 
GGTGACATTA AAACAATCCT AACGGTCCAG AGACATCTTA TCGCTGTGGA AGATGATAAC 
GGAGATACAC CGCTGCATGC TGCAATCATC AACAAGAAGT ATGATGTGAC ACATGCGCTT 
CTCTCGGCTG TAATCAAGAT TCCAGATCAG ATCATCGTTA ACCAGACTAA CCATTTGAAA 
CAGACTCCCC TCCACCTGGC CGTCATCACA AATCAATCCA AGATGGTGGA GGTCCTTTTG 
AGATGCGGGG CCAACCCCAA CCTCTGTGAT CATGAGGGTA ATACCCCCCT CCATCTGGCC 
ACTAGGATGG GTATGGCAGA AGGTGTAAAC TTCCTGGTGC GTGGCCCCAA GGCTAAGGCA 
GCCATTAAAC CCATCAAGAC CGACATTAAT CCAACAAACT ATGAAGGCCT TGCCCCAGTC 
CACCTGGCTG TGATCGCTAA GAACTTGGAT ATCCTTAAGG CACTTGTGTC ATCAGGAGCG 
GATGTCAACG TAGCTGATGG GAAGACCGGC CGAACCGCCC TCCACTATGC CGTGGAAGTC 
GAGAGCTTCC CAATTCTGGG CTACCTCCTG ATCGAAGCCA AGGTCGACAT CAATGCCGTG 
ACCTTCTGTG GGGACTCGGC CCTTCACCTG GCCAGTAGCC TGGATCTGAG GGCGGTGGCC 
ACGCTCCTCA TCGCAGCAGG AGCTGACCCT AAGCTGGAGA ACGCCGACCT GGGGGACGAC 
AGTGACAGCG ATGTCGACGG GGAGGATGAC GATGGTGAGA AGGATGAGGA GAAGAAGGAG 
GAGGGAGTGG AAGAGGAGGA GAAGGAGAAG CATGGGAAGA CACCCTATCA GCTGGCCACA 
AGTGCTAAGA TGAAAGAAGT CTTGTCTGGA CATTCCACCA GGAGAGTAGA GGAGGAGGAT 
TTTGACTACA TCGAGACAGG AAGTGCCATC AGTTCCGTTG GGTCAATCCA ATCAGAGGAC 
CACATGAAAG TCAATAGCTG GGACCAGGGC TACTTCTCAG GGAAGTCCAG CTCCTCTGTC 
TCTAACCTGG AACTAAAGCG AACCTGGCCC AGAGTTAAAA GCAGAATGCT AGGGAGCTCC 
CTCATCACAT CTAAAGGAGA CATTGGCCTG TTGTCTGAAA CCACCACCGA GAAGCTAGGC 
TCTATGTTGG ATGATAATTA CCCTACTACA CAGAGCTGGT TTACCCTGGC CAATCGGCTC 
GGACTCTCCA ACATGCTCAA CTTTCTCAAG CTTGTCCCAA GCCCCACTGC AGTCATTCTC 
AAACAGTTTG AGGCTATGGA TGGAACCATT AAGGAGCTCC GAGACGTCCT CTATTCAATG 
AATCACATAG AAGCTGTGGC CTTACTGGAT CAGGCCGTCT CATGGAGGAA AGAGAAAGCT 
GATGAGGCTC ATATGTACAG TTCGATGCAC CAGTCTTTGG GTAAGATGAA TTTGTCCGAC 
GCTCACATGC ATCAACATCA ATTCGTTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030834883.1
CDS	440..3832
Translation	MMEEKSDSVQSVEIPEDMLQQLIHQNGQVGLPMSGAENDFDSNDLMHLPVMKGLQEEVSKPHLKILEQPRQRGFRFRYGCEGPSHGGLPGQNSQRGKRSFPSVEICNYKGSARIVVSLVTNEETPRPHAHSLVGKHCKDGLCTVQVGPKDMTARSEMSFPNLGILHVTRKDVVPTLKTRILAQHRLYKDLINNSTPGESHWTEPSDAEIEKKAKEMAKDMDLSVVRLCFQTYLPDISGHFTRPLDPVISVPVFDSKAPNATTLKICRMDKSAGCCTGGEEVYLLCDKVQKEDIQVKFFEISADGQMVWQSLAEFGPTDVHRQYAIVFKTPAYKDINIDKPVYVHVQLKRKSDNETSDPKPFTFHPQVPDREGILRKRKKHLAHFNEYSSTYQQGGLGGSSGMGGGGGSTSFNFNNPNMGYGANTYNFSGNQMTSSTSQPQSVPQQIPSHQSGVVATGNSSQQMVYTTSTGAQNHQPLPSIATLARKPQEQPQQQQFQLQQQLQQQQQQQQQRQQQQQRQQQQQQPMQMDFQMPPIQGSAMGGQAGGDRVKMESESKYCGNMDTAEGFQLDSNTLRDCGPLPDLKELQDTGVLDLIPKGISRPKDMVEKNIQTDDITIDSDSMAWHVAQVTANALHDYAATGDIKTILTVQRHLIAVEDDNGDTPLHAAIINKKYDVTHALLSAVIKIPDQIIVNQTNHLKQTPLHLAVITNQSKMVEVLLRCGANPNLCDHEGNTPLHLATRMGMAEGVNFLVRGPKAKAAIKPIKTDINPTNYEGLAPVHLAVIAKNLDILKALVSSGADVNVADGKTGRTALHYAVEVESFPILGYLLIEAKVDINAVTFCGDSALHLASSLDLRAVATLLIAAGADPKLENADLGDDSDSDVDGEDDDGEKDEEKKEEGVEEEEKEKHGKTPYQLATSAKMKEVLSGHSTRRVEEEDFDYIETGSAISSVGSIQSEDHMKVNSWDQGYFSGKSSSSVSNLELKRTWPRVKSRMLGSSLITSKGDIGLLSETTTEKLGSMLDDNYPTTQSWFTLANRLGLSNMLNFLKLVPSPTAVILKQFEAMDGTIKELRDVLYSMNHIEAVALLDQAVSWRKEKADEAHMYSSMHQSLGKMNLSDAHMHQHQFVY

Target ORF information:

RefSeq Version	XM_030979023.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus NFkB protein (NFkB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030979023.1

ORF Insert Sequence:

ATGATGGAGG AGAAATCTGA CTCGGTGCAG TCTGTGGAGA TTCCAGAGGA CATGTTACAG 
CAGCTCATAC ACCAGAACGG TCAAGTGGGC TTACCCATGT CTGGAGCTGA GAATGATTTC 
GATAGCAACG ACCTGATGCA TCTACCTGTA ATGAAAGGTC TTCAGGAAGA GGTGTCCAAA 
CCTCATCTCA AGATATTGGA ACAACCCAGA CAGCGAGGAT TTCGGTTTCG ATATGGATGT 
GAGGGTCCAT CACACGGAGG ATTACCAGGC CAGAACAGTC AGAGAGGCAA AAGATCCTTC 
CCTTCCGTTG AGATCTGTAA TTACAAAGGC TCTGCCCGTA TAGTTGTCTC CTTGGTAACC 
AATGAAGAGA CGCCCCGCCC CCACGCCCAC TCTCTGGTAG GGAAGCACTG TAAGGATGGG 
CTCTGCACAG TCCAGGTCGG CCCCAAGGAT ATGACAGCAA GGTCAGAAAT GAGCTTCCCA 
AACCTGGGCA TCCTGCACGT GACGCGCAAG GACGTCGTCC CGACCCTCAA GACCCGTATC 
TTGGCCCAGC ATCGTCTCTA CAAGGATCTG ATCAACAACT CTACACCAGG GGAAAGCCAC 
TGGACGGAGC CGTCCGATGC AGAGATCGAG AAGAAAGCCA AGGAGATGGC TAAGGATATG 
GATCTTAGCG TCGTCCGGCT CTGCTTCCAG ACCTATCTGC CGGACATCTC AGGACACTTC 
ACCCGACCCC TTGACCCTGT CATATCAGTG CCAGTCTTTG ATAGCAAGGC ACCCAACGCC 
ACGACCCTCA AGATCTGTCG CATGGACAAG AGTGCAGGAT GCTGTACAGG AGGAGAGGAG 
GTCTATCTGC TCTGTGATAA GGTCCAGAAG GAGGACATAC AGGTCAAGTT CTTTGAGATC 
TCTGCCGATG GTCAGATGGT CTGGCAGAGC TTAGCAGAGT TTGGTCCAAC CGATGTTCAT 
AGACAGTATG CAATTGTATT TAAGACTCCA GCCTACAAAG ATATAAACAT TGATAAACCT 
GTGTATGTGC ACGTTCAGCT CAAGAGGAAA TCAGATAATG AGACCAGCGA TCCCAAGCCT 
TTCACCTTCC ACCCTCAAGT ACCAGATCGG GAAGGAATCC TTCGTAAGCG TAAGAAGCAC 
TTGGCTCACT TTAATGAGTA TAGTAGCACC TACCAGCAGG GTGGCCTAGG GGGAAGCAGT 
GGCATGGGAG GAGGAGGAGG ATCAACATCT TTTAATTTCA ACAACCCTAA CATGGGGTAT 
GGAGCTAATA CCTACAACTT TAGCGGCAAC CAAATGACCT CTTCAACATC GCAGCCACAA 
TCAGTGCCGC AACAAATCCC TAGCCACCAG TCTGGGGTCG TTGCTACCGG CAACTCCTCT 
CAGCAGATGG TATACACAAC ATCGACCGGT GCACAGAACC ACCAGCCCTT GCCTAGCATT 
GCAACACTAG CAAGGAAACC CCAAGAGCAG CCACAGCAAC AACAATTCCA GTTGCAGCAA 
CAACTGCAGC AGCAACAGCA GCAACAACAG CAGCGACAGC AGCAACAGCA GCGACAGCAG 
CAACAGCAGC AGCCGATGCA GATGGATTTC CAGATGCCAC CTATCCAAGG CAGTGCGATG 
GGAGGCCAGG CTGGTGGAGA TCGCGTTAAG ATGGAGAGTG AGAGCAAATA CTGTGGCAAC 
ATGGATACAG CAGAAGGATT TCAGCTGGAC TCCAATACAT TACGTGATTG TGGGCCGCTA 
CCAGACCTCA AAGAACTTCA AGATACAGGT GTCTTGGATC TCATCCCAAA GGGTATCTCA 
AGACCAAAGG ATATGGTGGA GAAAAATATT CAAACTGATG ACATAACAAT AGACAGTGAT 
TCCATGGCAT GGCATGTTGC CCAGGTAACA GCCAATGCTC TTCATGACTA CGCAGCTACG 
GGTGACATTA AAACAATCCT AACGGTCCAG AGACATCTTA TCGCTGTGGA AGATGATAAC 
GGAGATACAC CGCTGCATGC TGCAATCATC AACAAGAAGT ATGATGTGAC ACATGCGCTT 
CTCTCGGCTG TAATCAAGAT TCCAGATCAG ATCATCGTTA ACCAGACTAA CCATTTGAAA 
CAGACTCCCC TCCACCTGGC CGTCATCACA AATCAATCCA AGATGGTGGA GGTCCTTTTG 
AGATGCGGGG CCAACCCCAA CCTCTGTGAT CATGAGGGTA ATACCCCCCT CCATCTGGCC 
ACTAGGATGG GTATGGCAGA AGGTGTAAAC TTCCTGGTGC GTGGCCCCAA GGCTAAGGCA 
GCCATTAAAC CCATCAAGAC CGACATTAAT CCAACAAACT ATGAAGGCCT TGCCCCAGTC 
CACCTGGCTG TGATCGCTAA GAACTTGGAT ATCCTTAAGG CACTTGTGTC ATCAGGAGCG 
GATGTCAACG TAGCTGATGG GAAGACCGGC CGAACCGCCC TCCACTATGC CGTGGAAGTC 
GAGAGCTTCC CAATTCTGGG CTACCTCCTG ATCGAAGCCA AGGTCGACAT CAATGCCGTG 
ACCTTCTGTG GGGACTCGGC CCTTCACCTG GCCAGTAGCC TGGATCTGAG GGCGGTGGCC 
ACGCTCCTCA TCGCAGCAGG AGCTGACCCT AAGCTGGAGA ACGCCGACCT GGGGGACGAC 
AGTGACAGCG ATGTCGACGG GGAGGATGAC GATGGTGAGA AGGATGAGGA GAAGAAGGAG 
GAGGGAGTGG AAGAGGAGGA GAAGGAGAAG CATGGGAAGA CACCCTATCA GCTGGCCACA 
AGTGCTAAGA TGAAAGAAGT CTTGTCTGGA CATTCCACCA GGAGAGTAGA GGAGGAGGAT 
TTTGACTACA TCGAGACAGG AAGTGCCATC AGTTCCGTTG GGTCAATCCA ATCAGAGGAC 
CACATGAAAG TCAATAGCTG GGACCAGGGC TACTTCTCAG GGAAGTCCAG CTCCTCTGTC 
TCTAACCTGG AACTAAAGCG AACCTGGCCC AGAGTTAAAA GCAGAATGCT AGGGAGCTCC 
CTCATCACAT CTAAAGGAGA CATTGGCCTG TTGTCTGAAA CCACCACCGA GAAGCTAGGC 
TCTATGTTGG ATGATAATTA CCCTACTACA CAGAGCTGGT TTACCCTGGC CAATCGGCTC 
GGACTCTCCA ACATGCTCAA CTTTCTCAAG CTTGTCCCAA GCCCCACTGC AGTCATTCTC 
AAACAGTTTG AGGCTATGGA TGGAACCATT AAGGAGCTCC GAGACGTCCT CTATTCAATG 
AATCACATAG AAGCTGTGGC CTTACTGGAT CAGGCCGTCT CATGGAGGAA AGAGAAAGCT 
GATGAGGCTC ATATGTACAG TTCGATGCAC CAGTCTTTGG GTAAGATGAA TTTGTCCGAC 
GCTCACATGC ATCAACATCA ATTCGTTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFkB ( XM_030979024.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030834884.1 Strongylocentrotus purpuratus NFkB protein (NFkB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSd45309
Clone ID Related Accession (Same CDS sequence)	XM_030979024.1
Accession Version	XM_030979024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	NFkB protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145595.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGG AGAAATCTGA CTCGGTGCAG TCTGTGGAGA TTCCAGAGGA CATGTTACAG 
CAGCTCATAC ACCAGAACGG TCAAGTGGGC TTACCCATGT CTGGAGCTGA GAATGATTTC 
GATAGCAACG ACCTGATGCA TCTACCTGTA ATGAAAGGTC TTCAGGAAGA GGTGTCCAAA 
CCTCATCTCA AGATATTGGA ACAACCCAGA CAGCGAGGAT TTCGGTTTCG ATATGGATGT 
GAGGGTCCAT CACACGGAGG ATTACCAGGC CAGAACAGTC AGAGAGGCAA AAGATCCTTC 
CCTTCCGTTG AGATCTGTAA TTACAAAGGC TCTGCCCGTA TAGTTGTCTC CTTGGTAACC 
AATGAAGAGA CGCCCCGCCC CCACGCCCAC TCTCTGGTAG GGAAGCACTG TAAGGATGGG 
CTCTGCACAG TCCAGGTCGG CCCCAAGGAT ATGACAGCAA GGTCAGAAAT GAGCTTCCCA 
AACCTGGGCA TCCTGCACGT GACGCGCAAG GACGTCGTCC CGACCCTCAA GACCCGTATC 
TTGGCCCAGC ATCGTCTCTA CAAGGATCTG ATCAACAACT CTACACCAGG GGAAAGCCAC 
TGGACGGAGC CGTCCGATGC AGAGATCGAG AAGAAAGCCA AGGAGATGGC TAAGGATATG 
GATCTTAGCG TCGTCCGGCT CTGCTTCCAG ACCTATCTGC CGGACATCTC AGGACACTTC 
ACCCGACCCC TTGACCCTGT CATATCAGTG CCAGTCTTTG ATAGCAAGGC ACCCAACGCC 
ACGACCCTCA AGATCTGTCG CATGGACAAG AGTGCAGGAT GCTGTACAGG AGGAGAGGAG 
GTCTATCTGC TCTGTGATAA GGTCCAGAAG GAGGACATAC AGGTCAAGTT CTTTGAGATC 
TCTGCCGATG GTCAGATGGT CTGGCAGAGC TTAGCAGAGT TTGGTCCAAC CGATGTTCAT 
AGACAGTATG CAATTGTATT TAAGACTCCA GCCTACAAAG ATATAAACAT TGATAAACCT 
GTGTATGTGC ACGTTCAGCT CAAGAGGAAA TCAGATAATG AGACCAGCGA TCCCAAGCCT 
TTCACCTTCC ACCCTCAAGT ACCAGATCGG GAAGGAATCC TTCGTAAGCG TAAGAAGCAC 
TTGGCTCACT TTAATGAGTA TAGTAGCACC TACCAGCAGG GTGGCCTAGG GGGAAGCAGT 
GGCATGGGAG GAGGAGGAGG ATCAACATCT TTTAATTTCA ACAACCCTAA CATGGGGTAT 
GGAGCTAATA CCTACAACTT TAGCGGCAAC CAAATGACCT CTTCAACATC GCAGCCACAA 
TCAGTGCCGC AACAAATCCC TAGCCACCAG TCTGGGGTCG TTGCTACCGG CAACTCCTCT 
CAGCAGATGG TATACACAAC ATCGACCGGT GCACAGAACC ACCAGCCCTT GCCTAGCATT 
GCAACACTAG CAAGGAAACC CCAAGAGCAG CCACAGCAAC AACAATTCCA GTTGCAGCAA 
CAACTGCAGC AGCAACAGCA GCAACAACAG CAGCGACAGC AGCAACAGCA GCGACAGCAG 
CAACAGCAGC AGCCGATGCA GATGGATTTC CAGATGCCAC CTATCCAAGG CAGTGCGATG 
GGAGGCCAGG CTGGTGGAGA TCGCGTTAAG ATGGAGAGTG AGAGCAAATA CTGTGGCAAC 
ATGGATACAG CAGGATTTCA GCTGGACTCC AATACATTAC GTGATTGTGG GCCGCTACCA 
GACCTCAAAG AACTTCAAGA TACAGGTGTC TTGGATCTCA TCCCAAAGGG TATCTCAAGA 
CCAAAGGATA TGGTGGAGAA AAATATTCAA ACTGATGACA TAACAATAGA CAGTGATTCC 
ATGGCATGGC ATGTTGCCCA GGTAACAGCC AATGCTCTTC ATGACTACGC AGCTACGGGT 
GACATTAAAA CAATCCTAAC GGTCCAGAGA CATCTTATCG CTGTGGAAGA TGATAACGGA 
GATACACCGC TGCATGCTGC AATCATCAAC AAGAAGTATG ATGTGACACA TGCGCTTCTC 
TCGGCTGTAA TCAAGATTCC AGATCAGATC ATCGTTAACC AGACTAACCA TTTGAAACAG 
ACTCCCCTCC ACCTGGCCGT CATCACAAAT CAATCCAAGA TGGTGGAGGT CCTTTTGAGA 
TGCGGGGCCA ACCCCAACCT CTGTGATCAT GAGGGTAATA CCCCCCTCCA TCTGGCCACT 
AGGATGGGTA TGGCAGAAGG TGTAAACTTC CTGGTGCGTG GCCCCAAGGC TAAGGCAGCC 
ATTAAACCCA TCAAGACCGA CATTAATCCA ACAAACTATG AAGGCCTTGC CCCAGTCCAC 
CTGGCTGTGA TCGCTAAGAA CTTGGATATC CTTAAGGCAC TTGTGTCATC AGGAGCGGAT 
GTCAACGTAG CTGATGGGAA GACCGGCCGA ACCGCCCTCC ACTATGCCGT GGAAGTCGAG 
AGCTTCCCAA TTCTGGGCTA CCTCCTGATC GAAGCCAAGG TCGACATCAA TGCCGTGACC 
TTCTGTGGGG ACTCGGCCCT TCACCTGGCC AGTAGCCTGG ATCTGAGGGC GGTGGCCACG 
CTCCTCATCG CAGCAGGAGC TGACCCTAAG CTGGAGAACG CCGACCTGGG GGACGACAGT 
GACAGCGATG TCGACGGGGA GGATGACGAT GGTGAGAAGG ATGAGGAGAA GAAGGAGGAG 
GGAGTGGAAG AGGAGGAGAA GGAGAAGCAT GGGAAGACAC CCTATCAGCT GGCCACAAGT 
GCTAAGATGA AAGAAGTCTT GTCTGGACAT TCCACCAGGA GAGTAGAGGA GGAGGATTTT 
GACTACATCG AGACAGGAAG TGCCATCAGT TCCGTTGGGT CAATCCAATC AGAGGACCAC 
ATGAAAGTCA ATAGCTGGGA CCAGGGCTAC TTCTCAGGGA AGTCCAGCTC CTCTGTCTCT 
AACCTGGAAC TAAAGCGAAC CTGGCCCAGA GTTAAAAGCA GAATGCTAGG GAGCTCCCTC 
ATCACATCTA AAGGAGACAT TGGCCTGTTG TCTGAAACCA CCACCGAGAA GCTAGGCTCT 
ATGTTGGATG ATAATTACCC TACTACACAG AGCTGGTTTA CCCTGGCCAA TCGGCTCGGA 
CTCTCCAACA TGCTCAACTT TCTCAAGCTT GTCCCAAGCC CCACTGCAGT CATTCTCAAA 
CAGTTTGAGG CTATGGATGG AACCATTAAG GAGCTCCGAG ACGTCCTCTA TTCAATGAAT 
CACATAGAAG CTGTGGCCTT ACTGGATCAG GCCGTCTCAT GGAGGAAAGA GAAAGCTGAT 
GAGGCTCATA TGTACAGTTC GATGCACCAG TCTTTGGGTA AGATGAATTT GTCCGACGCT 
CACATGCATC AACATCAATT CGTTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030834884.1
CDS	440..3829
Translation	MMEEKSDSVQSVEIPEDMLQQLIHQNGQVGLPMSGAENDFDSNDLMHLPVMKGLQEEVSKPHLKILEQPRQRGFRFRYGCEGPSHGGLPGQNSQRGKRSFPSVEICNYKGSARIVVSLVTNEETPRPHAHSLVGKHCKDGLCTVQVGPKDMTARSEMSFPNLGILHVTRKDVVPTLKTRILAQHRLYKDLINNSTPGESHWTEPSDAEIEKKAKEMAKDMDLSVVRLCFQTYLPDISGHFTRPLDPVISVPVFDSKAPNATTLKICRMDKSAGCCTGGEEVYLLCDKVQKEDIQVKFFEISADGQMVWQSLAEFGPTDVHRQYAIVFKTPAYKDINIDKPVYVHVQLKRKSDNETSDPKPFTFHPQVPDREGILRKRKKHLAHFNEYSSTYQQGGLGGSSGMGGGGGSTSFNFNNPNMGYGANTYNFSGNQMTSSTSQPQSVPQQIPSHQSGVVATGNSSQQMVYTTSTGAQNHQPLPSIATLARKPQEQPQQQQFQLQQQLQQQQQQQQQRQQQQQRQQQQQQPMQMDFQMPPIQGSAMGGQAGGDRVKMESESKYCGNMDTAGFQLDSNTLRDCGPLPDLKELQDTGVLDLIPKGISRPKDMVEKNIQTDDITIDSDSMAWHVAQVTANALHDYAATGDIKTILTVQRHLIAVEDDNGDTPLHAAIINKKYDVTHALLSAVIKIPDQIIVNQTNHLKQTPLHLAVITNQSKMVEVLLRCGANPNLCDHEGNTPLHLATRMGMAEGVNFLVRGPKAKAAIKPIKTDINPTNYEGLAPVHLAVIAKNLDILKALVSSGADVNVADGKTGRTALHYAVEVESFPILGYLLIEAKVDINAVTFCGDSALHLASSLDLRAVATLLIAAGADPKLENADLGDDSDSDVDGEDDDGEKDEEKKEEGVEEEEKEKHGKTPYQLATSAKMKEVLSGHSTRRVEEEDFDYIETGSAISSVGSIQSEDHMKVNSWDQGYFSGKSSSSVSNLELKRTWPRVKSRMLGSSLITSKGDIGLLSETTTEKLGSMLDDNYPTTQSWFTLANRLGLSNMLNFLKLVPSPTAVILKQFEAMDGTIKELRDVLYSMNHIEAVALLDQAVSWRKEKADEAHMYSSMHQSLGKMNLSDAHMHQHQFVY

Target ORF information:

RefSeq Version	XM_030979024.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus NFkB protein (NFkB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030979024.1

ORF Insert Sequence:

ATGATGGAGG AGAAATCTGA CTCGGTGCAG TCTGTGGAGA TTCCAGAGGA CATGTTACAG 
CAGCTCATAC ACCAGAACGG TCAAGTGGGC TTACCCATGT CTGGAGCTGA GAATGATTTC 
GATAGCAACG ACCTGATGCA TCTACCTGTA ATGAAAGGTC TTCAGGAAGA GGTGTCCAAA 
CCTCATCTCA AGATATTGGA ACAACCCAGA CAGCGAGGAT TTCGGTTTCG ATATGGATGT 
GAGGGTCCAT CACACGGAGG ATTACCAGGC CAGAACAGTC AGAGAGGCAA AAGATCCTTC 
CCTTCCGTTG AGATCTGTAA TTACAAAGGC TCTGCCCGTA TAGTTGTCTC CTTGGTAACC 
AATGAAGAGA CGCCCCGCCC CCACGCCCAC TCTCTGGTAG GGAAGCACTG TAAGGATGGG 
CTCTGCACAG TCCAGGTCGG CCCCAAGGAT ATGACAGCAA GGTCAGAAAT GAGCTTCCCA 
AACCTGGGCA TCCTGCACGT GACGCGCAAG GACGTCGTCC CGACCCTCAA GACCCGTATC 
TTGGCCCAGC ATCGTCTCTA CAAGGATCTG ATCAACAACT CTACACCAGG GGAAAGCCAC 
TGGACGGAGC CGTCCGATGC AGAGATCGAG AAGAAAGCCA AGGAGATGGC TAAGGATATG 
GATCTTAGCG TCGTCCGGCT CTGCTTCCAG ACCTATCTGC CGGACATCTC AGGACACTTC 
ACCCGACCCC TTGACCCTGT CATATCAGTG CCAGTCTTTG ATAGCAAGGC ACCCAACGCC 
ACGACCCTCA AGATCTGTCG CATGGACAAG AGTGCAGGAT GCTGTACAGG AGGAGAGGAG 
GTCTATCTGC TCTGTGATAA GGTCCAGAAG GAGGACATAC AGGTCAAGTT CTTTGAGATC 
TCTGCCGATG GTCAGATGGT CTGGCAGAGC TTAGCAGAGT TTGGTCCAAC CGATGTTCAT 
AGACAGTATG CAATTGTATT TAAGACTCCA GCCTACAAAG ATATAAACAT TGATAAACCT 
GTGTATGTGC ACGTTCAGCT CAAGAGGAAA TCAGATAATG AGACCAGCGA TCCCAAGCCT 
TTCACCTTCC ACCCTCAAGT ACCAGATCGG GAAGGAATCC TTCGTAAGCG TAAGAAGCAC 
TTGGCTCACT TTAATGAGTA TAGTAGCACC TACCAGCAGG GTGGCCTAGG GGGAAGCAGT 
GGCATGGGAG GAGGAGGAGG ATCAACATCT TTTAATTTCA ACAACCCTAA CATGGGGTAT 
GGAGCTAATA CCTACAACTT TAGCGGCAAC CAAATGACCT CTTCAACATC GCAGCCACAA 
TCAGTGCCGC AACAAATCCC TAGCCACCAG TCTGGGGTCG TTGCTACCGG CAACTCCTCT 
CAGCAGATGG TATACACAAC ATCGACCGGT GCACAGAACC ACCAGCCCTT GCCTAGCATT 
GCAACACTAG CAAGGAAACC CCAAGAGCAG CCACAGCAAC AACAATTCCA GTTGCAGCAA 
CAACTGCAGC AGCAACAGCA GCAACAACAG CAGCGACAGC AGCAACAGCA GCGACAGCAG 
CAACAGCAGC AGCCGATGCA GATGGATTTC CAGATGCCAC CTATCCAAGG CAGTGCGATG 
GGAGGCCAGG CTGGTGGAGA TCGCGTTAAG ATGGAGAGTG AGAGCAAATA CTGTGGCAAC 
ATGGATACAG CAGGATTTCA GCTGGACTCC AATACATTAC GTGATTGTGG GCCGCTACCA 
GACCTCAAAG AACTTCAAGA TACAGGTGTC TTGGATCTCA TCCCAAAGGG TATCTCAAGA 
CCAAAGGATA TGGTGGAGAA AAATATTCAA ACTGATGACA TAACAATAGA CAGTGATTCC 
ATGGCATGGC ATGTTGCCCA GGTAACAGCC AATGCTCTTC ATGACTACGC AGCTACGGGT 
GACATTAAAA CAATCCTAAC GGTCCAGAGA CATCTTATCG CTGTGGAAGA TGATAACGGA 
GATACACCGC TGCATGCTGC AATCATCAAC AAGAAGTATG ATGTGACACA TGCGCTTCTC 
TCGGCTGTAA TCAAGATTCC AGATCAGATC ATCGTTAACC AGACTAACCA TTTGAAACAG 
ACTCCCCTCC ACCTGGCCGT CATCACAAAT CAATCCAAGA TGGTGGAGGT CCTTTTGAGA 
TGCGGGGCCA ACCCCAACCT CTGTGATCAT GAGGGTAATA CCCCCCTCCA TCTGGCCACT 
AGGATGGGTA TGGCAGAAGG TGTAAACTTC CTGGTGCGTG GCCCCAAGGC TAAGGCAGCC 
ATTAAACCCA TCAAGACCGA CATTAATCCA ACAAACTATG AAGGCCTTGC CCCAGTCCAC 
CTGGCTGTGA TCGCTAAGAA CTTGGATATC CTTAAGGCAC TTGTGTCATC AGGAGCGGAT 
GTCAACGTAG CTGATGGGAA GACCGGCCGA ACCGCCCTCC ACTATGCCGT GGAAGTCGAG 
AGCTTCCCAA TTCTGGGCTA CCTCCTGATC GAAGCCAAGG TCGACATCAA TGCCGTGACC 
TTCTGTGGGG ACTCGGCCCT TCACCTGGCC AGTAGCCTGG ATCTGAGGGC GGTGGCCACG 
CTCCTCATCG CAGCAGGAGC TGACCCTAAG CTGGAGAACG CCGACCTGGG GGACGACAGT 
GACAGCGATG TCGACGGGGA GGATGACGAT GGTGAGAAGG ATGAGGAGAA GAAGGAGGAG 
GGAGTGGAAG AGGAGGAGAA GGAGAAGCAT GGGAAGACAC CCTATCAGCT GGCCACAAGT 
GCTAAGATGA AAGAAGTCTT GTCTGGACAT TCCACCAGGA GAGTAGAGGA GGAGGATTTT 
GACTACATCG AGACAGGAAG TGCCATCAGT TCCGTTGGGT CAATCCAATC AGAGGACCAC 
ATGAAAGTCA ATAGCTGGGA CCAGGGCTAC TTCTCAGGGA AGTCCAGCTC CTCTGTCTCT 
AACCTGGAAC TAAAGCGAAC CTGGCCCAGA GTTAAAAGCA GAATGCTAGG GAGCTCCCTC 
ATCACATCTA AAGGAGACAT TGGCCTGTTG TCTGAAACCA CCACCGAGAA GCTAGGCTCT 
ATGTTGGATG ATAATTACCC TACTACACAG AGCTGGTTTA CCCTGGCCAA TCGGCTCGGA 
CTCTCCAACA TGCTCAACTT TCTCAAGCTT GTCCCAAGCC CCACTGCAGT CATTCTCAAA 
CAGTTTGAGG CTATGGATGG AACCATTAAG GAGCTCCGAG ACGTCCTCTA TTCAATGAAT 
CACATAGAAG CTGTGGCCTT ACTGGATCAG GCCGTCTCAT GGAGGAAAGA GAAAGCTGAT 
GAGGCTCATA TGTACAGTTC GATGCACCAG TCTTTGGGTA AGATGAATTT GTCCGACGCT 
CACATGCATC AACATCAATT CGTTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Genome research22(10)2079-87(2012 Oct)
Tu Q,Cameron RA,Worley KC,Gibbs RA,Davidson EH

Origins of immunity: transcription factors and homologues of effector genes of the vertebrate immune system expressed in sea urchin coelomocytes.
Immunogenetics49(9)773-86(1999 Aug)
Pancer Z,Rast JP,Davidson EH

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NFkB cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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