wnt1 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	wnt1
Entrez Gene ID	373498
Full Name	Wnt-1 protein
Synonyms	WNT-1
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001123500.1	NP_001116972.1	protein Wnt-1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Tu Q, Cameron RA, Worley KC, Gibbs RA, Davidson EH
Genome research22(10)2079-87(2012 Oct)

Axial patterning interactions in the sea urchin embryo: suppression of nodal by Wnt1 signaling.
Wei Z, Range R, Angerer R, Angerer L
Development (Cambridge, England)139(9)1662-9(2012 May)

Phylogenetic relationships and developmental expression of three sea urchin Wnt genes.
Ferkowicz MJ, Stander MC, Raff RA
Molecular biology and evolution15(7)809-19(1998 Jul)

Diversification of the Wnt gene family on the ancestral lineage of vertebrates.
Sidow A
Proceedings of the National Academy of Sciences of the United States of America89(11)5098-102(1992 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following wnt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSd00032	NM_001123500.1 Latest version!	Strongylocentrotus purpuratus Wnt-1 protein (wnt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wnt1 ( NM_001123500.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> NP_001116972.1 Strongylocentrotus purpuratus Wnt-1 protein (wnt1), mRNA.

Price & Availability↑

CloneID	OSd00032
Clone ID Related Accession (Same CDS sequence)	NM_001123500.1
Accession Version	NM_001123500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556899200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	protein Wnt-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAGJ05117047.1 and AAGJ05117046.1. On Apr 22, 2008 this sequence version replaced XM_775661.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JT120395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01096960, SAMN01103178 [ECO:0000348] ##Evidence-Data-END##

ATGAAACTTG AGTGGTTTGT AGCGTTTTTC TTCATTCCTA CTGCCAGCTT ACTCGCTAAG 
AAGACTACAG CGGCATCAAG TACAAAATGG TGGAGCATGG TGCTATCACC AAGTATGACG 
AACAGTGTGA CAGACACACA AGGTATCATC AACATCATAG ATCCCAAATT GAAGATTTTA 
AACAAACGTC AAAGAAGATT GGTCTCACAG AATCGAGGGA CCTTAGCCGC TATTAACAGA 
GCAGTCAAGA TGGCTGTATC AGAGTGTCGA TACCAGTTTA AAGAGAGGAG GTGGAACTGT 
CCAACCTTTA ACAAAGATAG AGGTCCAGAG AGTCTGTTTG GAAAGATTTT GAATAGTGGT 
TGTCGAGAAA CATCATTCAT CTACTCAATA ACCAGCGCAG CAGTGACACA CTCCGTAGCG 
AGGTCGTGTA GCGAAGGAAC GATCGAGTCT TGCACGTGTG ATTACAAGTT CCGTGGTGAC 
AGCGGAAACG ATTGGGAGTG GGGCGGCTGC AGTGACAACG CTGACTTCGG CCACCGATTC 
GGCAAGAAGT TCGTAGACTC GGGCGAGAAA GGACGAGATC TCAGACATGC TATGAACCTA 
CATAATAACG AAGCAGGCAG AAAAACTGTA TCCAGTGAGA TGCGTCGAGA ATGTAAATGC 
CACGGGATGT CGGGGAGTTG TACCATAGAG ACATGTTGGA TGAGACTACC AACCTTCAGA 
ACGGTGGGTG AGCTCATCAA AGAGCGTTTC GACGGCGCCT CCCGCGTCAC CATGAGAAAT 
GACGGCAGTC CGAGCGACCG CGAAACCGAA TCAAGCTTCG TGCCTTACAA CCCATCTCAC 
AAGCAGCCAG CCTCACGAGA TCTGGTGTAT TTCGAAAACT CGCCTGATTT TTGCGAGCGG 
AATGATAAGT TCGGCACGCC CGGAACGAGA GGGCGGGAAT GCAACGCCAC GTCACTGGGC 
GTGGATGGGT GCGATCTTAT GTGCTGCTCG AGAGGGTCTA CGACAACTGA AATCAAGGTC 
AAAGAGAGAT GCAGTTGCAC GTTTCATTGG TGCTGTAAAG TAAAATGTGA AGAGTGCACG 
TCGTTTCGGA CCGTGCACAG ATGCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116972.1
CDS	506..1615
Translation	MKLEWFVAFFFIPTASLLAKKTTAASSTKWWSMVLSPSMTNSVTDTQGIINIIDPKLKILNKRQRRLVSQNRGTLAAINRAVKMAVSECRYQFKERRWNCPTFNKDRGPESLFGKILNSGCRETSFIYSITSAAVTHSVARSCSEGTIESCTCDYKFRGDSGNDWEWGGCSDNADFGHRFGKKFVDSGEKGRDLRHAMNLHNNEAGRKTVSSEMRRECKCHGMSGSCTIETCWMRLPTFRTVGELIKERFDGASRVTMRNDGSPSDRETESSFVPYNPSHKQPASRDLVYFENSPDFCERNDKFGTPGTRGRECNATSLGVDGCDLMCCSRGSTTTEIKVKERCSCTFHWCCKVKCEECTSFRTVHRCL

Target ORF information:

RefSeq Version	NM_001123500.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus Wnt-1 protein (wnt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123500.1

ORF Insert Sequence:

ATGAAACTTG AGTGGTTTGT AGCGTTTTTC TTCATTCCTA CTGCCAGCTT ACTCGCTAAG 
AAGACTACAG CGGCATCAAG TACAAAATGG TGGAGCATGG TGCTATCACC AAGTATGACG 
AACAGTGTGA CAGACACACA AGGTATCATC AACATCATAG ATCCCAAATT GAAGATTTTA 
AACAAACGTC AAAGAAGATT GGTCTCACAG AATCGAGGGA CCTTAGCCGC TATTAACAGA 
GCAGTCAAGA TGGCTGTATC AGAGTGTCGA TACCAGTTTA AAGAGAGGAG GTGGAACTGT 
CCAACCTTTA ACAAAGATAG AGGTCCAGAG AGTCTGTTTG GAAAGATTTT GAATAGTGGT 
TGTCGAGAAA CATCATTCAT CTACTCAATA ACCAGCGCAG CAGTGACACA CTCCGTAGCG 
AGGTCGTGTA GCGAAGGAAC GATCGAGTCT TGCACGTGTG ATTACAAGTT CCGTGGTGAC 
AGCGGAAACG ATTGGGAGTG GGGCGGCTGC AGTGACAACG CTGACTTCGG CCACCGATTC 
GGCAAGAAGT TCGTAGACTC GGGCGAGAAA GGACGAGATC TCAGACATGC TATGAACCTA 
CATAATAACG AAGCAGGCAG AAAAACTGTA TCCAGTGAGA TGCGTCGAGA ATGTAAATGC 
CACGGGATGT CGGGGAGTTG TACCATAGAG ACATGTTGGA TGAGACTACC AACCTTCAGA 
ACGGTGGGTG AGCTCATCAA AGAGCGTTTC GACGGCGCCT CCCGCGTCAC CATGAGAAAT 
GACGGCAGTC CGAGCGACCG CGAAACCGAA TCAAGCTTCG TGCCTTACAA CCCATCTCAC 
AAGCAGCCAG CCTCACGAGA TCTGGTGTAT TTCGAAAACT CGCCTGATTT TTGCGAGCGG 
AATGATAAGT TCGGCACGCC CGGAACGAGA GGGCGGGAAT GCAACGCCAC GTCACTGGGC 
GTGGATGGGT GCGATCTTAT GTGCTGCTCG AGAGGGTCTA CGACAACTGA AATCAAGGTC 
AAAGAGAGAT GCAGTTGCAC GTTTCATTGG TGCTGTAAAG TAAAATGTGA AGAGTGCACG 
TCGTTTCGGA CCGTGCACAG ATGCTTGTAG