REJ8 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	REJ8
Entrez Gene ID	100034240
Full Name	receptor for egg jelly 8
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_030976261.1	XP_030832121.1	receptor for egg jelly 8 isoform X1
XM_030976263.1	XP_030832123.1	receptor for egg jelly 8 isoform X2
XM_011673693.1	XP_011671995.1	receptor for egg jelly 8 isoform X1
XM_030976262.1	XP_030832122.1	receptor for egg jelly 8 isoform X1
NM_001081968.1	NP_001075437.1	receptor for egg jelly 8 precursor
XM_011673694.1	XP_011671996.1	receptor for egg jelly 8 isoform X2
XM_011673695.1	XP_011671997.1	receptor for egg jelly 8 isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Tu Q, Cameron RA, Worley KC, Gibbs RA, Davidson EH
Genome research22(10)2079-87(2012 Oct)

The 10 sea urchin receptor for egg jelly proteins (SpREJ) are members of the polycystic kidney disease-1 (PKD1) family.
Gunaratne HJ, Moy GW, Kinukawa M, Miyata S, Mah SA, Vacquier VD
BMC genomics8235(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following REJ8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the REJ8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd20380	NM_001081968.1 Latest version!	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSd30107	XM_011673693.1 Latest version!	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSd30108	XM_011673694.1 Latest version!	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSd30109	XM_011673695.1 Latest version!	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSd71217	XM_030976262.1 Latest version!	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSd71217	XM_030976261.1 Latest version!	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSd71218	XM_030976263.1 Latest version!	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

REJ8 ( NM_001081968.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> NP_001075437.1 Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), mRNA.

Price & Availability↑

CloneID	OSd20380
Clone ID Related Accession (Same CDS sequence)	NM_001081968.1
Accession Version	NM_001081968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569600000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	receptor for egg jelly 8 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF216328.1. ##Evidence-Data-START## Transcript exon combination :: EF216328.1 [ECO:0000332] ##Evidence-Data-END##

ATGAAGGCAC TTGTGGTGGT TGTCATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCATC 
ATCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GAATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATGG GACCCAAGCT TAGCTGGCTT TCCGATGGAG CAATGCGAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAGT 
TCTTGGTCCT TTCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCAATCGAAG ATTTGGATTT TAACTGGACC AACCCTATAT TCGATGATAC TGCGAACATG 
GTCAAGAATA TTGGACTCAT CCCGGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGAA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCGAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGAGTGG TTCTAAACGT CATCGTTGCC GAACAGGTTA TATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTCCAAA 
CACGAACCTG ATACAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGAAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATGTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAA TGAATTTCCC CGATTGGAGA 
GGCAATATGA GCGGGATTGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGACTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GCACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TGGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GACTGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAC CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTCTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
CAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AACTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGACATTGGC TTTGTTATTG GTACAATTTT CAGTTATATT 
GGAGGCTACC GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGATGATGAT 
GAACAACTTG ACATGGTAGC AAATAAAATG AAGGATAGTA TCTTCCGATT CTTCAACGAC 
CCATCATTCT GTCCTGCGAC GATGGACATT ATCAACAAGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTTTGTTTT GTTTGCCGAT 
GTTGAAGAAG AAGGAGACTT TGACCCTGCA GCCATCTTTA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGAG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGGTTGACGA TTTATGTCAA 
ACAGTATTGG CCTTTAATTC TGCAGAACTC CAAGATGTCT GCATCCACGA ACTATTTCCC 
TGGATTGAGG ACATTTTTGA CCACCATGAT GATGATGAAA TGAGGGAAGA AGAAAACGAA 
ATTGACTTTG ATTTCACAGG GATTCTCGAA ACGGGAGGCG AGGAATTCGG ATTCGACTTT 
CACGATTCTC ACCAAAGTTG CTCTCATCTT ACTGGTATTC TCTTTGATGA TACCGTCAGC 
CAAAACTTTC CAATCGACAG CGTTGTGGTA AGGGGAATAA GAGCTGTTGG TAAGATAGTA 
TCACCCATAT TCTGTGATTT TGAATCATTT CATGACCACC CAGATACTGT CTTGGAAGCT 
CCGTGTGCAA CTCCTGGACA AAACTGCTTT GATTGGTCAA GCCGAGGAGA AGACTTCTTC 
TATGAGCGGG AAGATATTGG CTTTGTCATT GGTACAATTT TCAGTTATAT TGGAGGCTAC 
AGAGATGGGG ACCACCTTTG TCACCAAATG GCCTTTGCAC TGGTTGATGA TGCACAGCTT 
GACATGGTAG CAAATAAAAT GAAGGATAGT ATCTTCCGAT TCTTCAACGA CCCATCATTC 
TGTCCTGCGA CGATGGACAT TATCAACAGG CTCATCGGAT TTGACGAGGA TGCTGCAAGT 
TTACTCGGCT TTGAGAATGC GGATGCCTTC TGCGGGAAGG TCAATCAAAT ATACAATGAA 
GATGGCGATT ATAACGTCAG TTTGGAGCCT CTCTATGTTT TGTTTGCCAA TGTTGAAGAA 
GAAGGAGAAT TTGACCCTGC AGCCATTATA AATGCCACAA CCGAGATGAT TAAATTCGCT 
GCCATGATCA TGCGTTCAGA CCACATTGGA GATGCCATTT CTAAAACATG CTACGTGTGG 
CAGGAATCTG CTGCAATGTT AAATATTTCA GAGATTGATG ATTTATGTCA ATTGGTTCTT 
GAGGACAATT CCGATGCTGT TTACCAAGTC TGCATGGGTG AACTTTTCCC ACTTATTGAG 
TCAGTCCTAG AAGATGAGAT GTATGATTTC AATGATAATC GTACAAACAC CGTTGTAGAT 
TTATCGAACA TTGAGATCTG TCTCGATAAT GGCGAGGTGA CTTTTGAGGC ACTTGAAAAT 
TTAGATATAT TGGCATATCT ACTACAAACC ACACACAAGC TCTATAATTT AACCGTGTTT 
GACGAGAGCA ACATTTGTAG AGCAGTGACC AGCGTAGTAG AATCTAGAAA TGACATTCGG 
ACACTGATAT CTGATTTTGC TGTGGAAATG ATGTCCCTCT TCATGCCGCT TGGTGCAGAA 
GTATGTTCTT GTTGGGATCA AGTTTTTAAT GATCTCTTCT CGGACTCCGA CGACTCGGAA 
TTTGGCGTAA ACCAATTCAA TGAGATAGTC CGGATTGCTG TGAACAGTAT TGGATTGGAC 
AGTCATCACG AGCTCTGTGA CCATCTCATT CATCTCAAGC CGGACAACAT GTCTGATTAC 
GCACAAACGG TGATTGAGGA AATCCTAGGT TTTACGGCTG ATCCTGAGAA GTGTGCCTGT 
ACCGCCAATC GTGTCATCGA TTTCATCCTT CCCTTGGTTA ATGTTTCAAT GGAGGAATTC 
AATAACTACC TTTACCAGTA CCTGGGATAC CATTCTGTGG ATCACGTGTG TAGTTTCGTG 
GCCCAATCCT TCTCGGGAGT AACAGTCGAC CCTTCAAGCA TAAACTGTAA CAGCCAACAA 
GAACCAACAA CCAAAGCTAC GACCACAACT CAATCATCAT TAGACAATAA TGGAAACTCA 
ACTCAATCAT CAATAGACAA TACTGGAAAC TCAACTCAAT CACCAATAGA CAATACTGGA 
GACTCGAATG AAACTTCGAC TGACGACGAA ACCTGTCTCT TCATCACTGA CTGCGCCGGT 
GTATGCAACG GTAACGCTAG TTTGGATTGC GCCCGAGAAT GCGGCGGCAG CGCGGCACTC 
GACTGCGCCT TCAAGTGCGG TGGCTCTGCG ACCACGAACG AATGTGGATG GTGCGTCGGT 
GGAGCTACGG AAAGACCAAG CACTTTAGGT TTCGACCAGT GTGGTTCCTG CATTGGGGAA 
GGCACTGCGG TCATCGACTG CAATGGTGAC TGTGACGGTA CTGCTTACCG CGATGCCTGT 
CAAGTCTGCG TAGGTGGCAA TACGAGTGTG GCTGAGGATG TAAGCGATCG TTTCTTGGAC 
TGCCTAGGTA TCTGTAATGG TAGTTTTGTG ACCAATGATT GTGATGAATG CGTGCTGCCA 
TTCAGCGATG GGACTATTTT CTCCACGGCG GATTGTAACG GTGACTGCAA TGGATTTGCC 
GACATAAATG AGTGTAGTTA CTGTGTTGGG GGTAATACCG GTAACTCTGT GGATACTGGA 
TTGTCTGCAT GTGGAGTCTG CGATGCAACG ATCGAACCAG ACTTCTGCCT CGGCTGTGAT 
GGCGTCGCTG ATAGTGGCCT AGTGACCGAT GCGTGTGATG TATGCGATGG GGATGGTAGC 
ACTTGTTTCA CCGTGGACAA TATAGCTCCC TCTATCATCC CAGCTAACAC CGACTATCAG 
GTTAAACTTC GTGGTGCCGG ATTCCAAGAT GCGACTCCCA AACAGTGTAA GTTCCTGGAT 
AGCAGCGGTG AGCAGGTCAG CGTGGTCGAT ATCAGCGATG AGGTGGATGA CGAGTTCAAC 
TGCACCGCTA ATCTTCCTCA AGGAACGTAT TCTGTTTACC TTTCGACGGA GTCAACGGAA 
CTATCAGAAG CATTCGTCAC CCTCTATGTC TATTCAGAAG TTATAATAAA CAGCTTATCC 
CAGAGCGAGT TTGATATAGA TGATAGTGTG ATTTCCATCT CTGTCAACAT GGTATCAACA 
AGCGGAGCCT TTACGGCATA CAAGGACTTG ACCACTCCAA AAGCTATCGT CTTCACAGAG 
ACCGAGAGAC TGATCTACGA AGGAACTTTT GGAGGAGACA ACGACGAGCT TCTCGAGTTT 
ACTGGTCCGA TGCTAACTAC CTCGGCTCAG GTTGTCGTCT ACCCGACGTT CAACGGAATC 
GACTACCTGG TAACTCCCGA TGATGCCGGT TTCACGATCA CCTACTACGC CCCTGCACCG 
GTCGCTCAGA GTCTGAAATT CTCCCCTACT GGACATCTTC TGGAACTCGA GTTCGACGTC 
GCAATCAACG AAGACTCAGT GACATCATGT GATGCAGTAT TCATGAATAC CACCAGCCTG 
GGTGGTGCTG CTGCCCAGTG CTTTGCATTT CCTTACCTAA AAACTATCTA TATACTACCC 
TTCAGTTCGG ACAGTGGAAA TCTTATTGAA CCAGATGATG AGCTAGTGCT GCGCGCTGAC 
GTTCTGAAAA AACGTGATGA GGAATACGCT CGGTACGCCA GCGGTACTCT AACCGCATCC 
TCCCCCGACG AGGCGGTTAC CGTCTACGCG GCTCTGACCG GTACAGAAGC AATTAGCAGC 
TGCGGTAATG TACGACTTAG TGCTCTGCAA TCCTTTGGAA ACGCAGGACG TTTTTTCACC 
TATCAGTGGT CTGTGACCAG TGGCGGTTCG ATCCCATCTG GCTTGACGGA TGATCTAACA 
GCCGCAGCGG AGTCCGCCTA TGTCACTCTC GATGGATCCT ACCTCGACGC GGACGTGCCG 
TATACATTCA GCATGACAGC CGAGAACTTC CTTGGAGGCA GTGACACAAA GACCTTGGAG 
GTGACTCGGT CTGCTCTCAA CAAACCAGAC GTCGTGATCT ACCCGGAAAG AGTTGATCCT 
AATAGTGCCC TTATCTCGCA AGCGTTCTAC CTCCGCGCTG CTGTGCGATT CTACGAGGCC 
TGCGGGGGAG CTGAGCAGAT GGAGTATGCC TGGTCTGTAG ATGATGATAG TATTGCTCTT 
AACCTACAAA CCATAAACAA ACCACTCCTG GATGTGGCAG CTGGTTCTTT ACCAGGCGAC 
TCCCAAGTGA CCTTTACACT GGAGGTCAAC CGGGAAAGTG ACCCCAGCAC GGTGACCACG 
CAGACCGTGA CCATTACCAC GGTCTACACC GACCTCGACG CCCTAATCGA TGGCGGGAGT 
GAGCGGACCA TCGGCGTCGA CAGTGAGTCA TTCACACTGG ATGGATCGTC GTCGTTTGAT 
CCCGACAGGG AAAATGAAGA GATGACCTAC CTCTGGACTT GTCGGAAGTC GAACACTGAG 
ACAGGCAGCT ACGAGCCTTG TGTATCAAGC GAGACACGTG AGGTGTTCCC GGCGGAAGCT 
GACTCCAAAA CAAGTACTCT CACGTTAACT TCCAACAATC TTTACTCGGA CTCCACCTAT 
CTGTTCACTC TGGAGATCAA CAAACTGTCA AGGACAGCAT CGACCAGTGT CACCATCCTT 
TCCAAGTCTG GCAATCCTCC AACGATCAGT CTCGATGAAT CCCTTCCTGA GAAGATAAAG 
AGCAGTAGTT CTCATACGCT ACGAGTATTG ATCAGCCATA CATCAGATAT TACCATTGAC 
TGGGCTACTT TCGACATAGA TCTTGGATAT GGATACGTTG ACTTTTCACA GCTGAATAGT 
CAGTCATTCC TTATACAAGG AGATACATCA CCCTTCATCA CATACGCCTT TGTAACTATC 
CCCACAGGAA CTTTGGAAGA GGATGTTGCC TACTCTTTCT CAGTGACGGT AACTGATACC 
GAAGGACAGG TGTCAGCTAG TAAATTGACT ACCAGCACGT ATGGAGGAGT ATCATCCTGT 
ACATTCAGTT TGGGTGATGG TTCAACCAGC TATCAAGAAC TTGACGAGAT CACTCTCCTA 
ACTCAGAACT GCGTGACTGA TGAAGAAGCT TACCCTCTGT CTTACCAAGT GTTCCGTGAG 
AAGGTCGAAT CATCTGTCAC CCGTTACAAC GCGATGACGG GTGCATTGGC AGAAGCGAGC 
AGCACTATAT TGGGTCTGCC GTCCTTTAGC GACAACCAGA ACACATTTGT TGTCAAGGTG 
TGCAATGATT TTGGAAGCTG TTCCTCGTAC AGTCTTACAT TGGACGTCAC TCCCATCGCA 
TCTTTCAGTC AAGAACAGTT TGATGAAAGT ATAACCAATC TGGTAGAACC ACAAAAGCTC 
TCAGGGAATT TCCTAGATGC GCTGGTAAAC TTCAACACCA TCACGTCAGT GGGTGTCGAC 
AGCACGAGTA GGAAACGGAG AGCAGTCTCG GAGACATCCA GCACAGCTAC TGAGCAGCTG 
GATTTACTTC AGAATGCCAT CTCGTCGACG GTCCTTGACA CGGCCACCGC CCAGACTCTG 
CTTGATCAGT CGGATGGCGT GGTCATTGCT GATCTCACAC TGTCTGATAT GGACATCTAC 
CTGGATATTT TAATTGAGCT CGTAGCTGTT TTCAGTAATG AAGGGCTTCC AGAGTCTAGC 
GCCGAGATCG TTCTTACAAA GACAAGCGAG ATCGCTGCTG AACTCGACCC TCAATACAAC 
TCGGACATGC TTGCCAAAAT TACGACCCTG AGAAACACAT TAATCCAGGG CCAGCTGAGT 
CAGATCCCAC TTGGAGGGGA CCCTACGGAG ACCAGGTCTG GTTCCGTTAC CACCAGGGCC 
TTCTATGCTA TCCCTAGTGA TACCCTCAGT ACTGCATCCA GCAACACCTC CTTCAACGTC 
AAGTTCGGAA GCGAGATCGA AAGCTTATAT GGTGCAAGTT GGTCGTGTGC TTCTGGAAGC 
ACTTGTTCTG GTGTTCAGAT CATGTTTACT CAGTACGACG ACGAGGTTGA TTACTACTCG 
ACCACGGACG AGGATAAGGC GAACAGAGCA TCTTCTATCC TTGAAATCGA TCTGCTTGAC 
CCGAGTGACA ATTCTGAGTT GAGTATTTCT GGACTGAGCA GCCCCATATC AATCAACATG 
ACCGCTTCAC GCCTCCAATC CGACAAAGTC TATGACTGCT ATTGGTGGGA CACTAGCAGC 
AGCTCTTGGA GCCAGGATGG ACTTACGACC GTCTACCCCT CTTCGGTCTT GTCTGAGTGC 
CAAACTACTC ATCTTTCAGC CTTCACCCTG ATCGCTGTTG ATTCTCCTAC TACGATTATG 
ATGGCTACCA CGATAGTTGA TGACGCCAGT ACTTCTACCG ATGCAGTAGC CTCGACTGCG 
ACAACGGAAA TCAGTGAGAG CGGCAGCAGT GGTCTAAGTT CTACCGTCAT CATCGTCATT 
GCCGTGGTCA TATCTGTCGT TTTGATCCTA ATTATTATCA TCGCCATCGT GGTCGTGATG 
TCGAAGAAGT CCTCCAAGGT CGGTAATGCT GATATCGAGA ACTCGAACGG CAATGGTGCC 
AGGAATGGGA CCAGCACCGC CGGTGGACTC GCTCCTGTTG AATCTGCGAA CCAGGCATTC 
AATGCCCCAC CAACAGCACC GGCCCACCCA ACCCATACTG TTCAAGTGGT CGCTGCCCCA 
GCTTGGGCAC CTGCTTCTCA AGAGCCTATC ATGGACGTTG CAGGCCTTCC TGGATCCCCA 
TCACCCCCTC CTGATGGACC CCATATTGAT ACCATCATAA AGGATGTTCA GAATGATAGT 
AAGGCCCAAA CTCCAATGGA TAGAGCCATG ACGCCCGTTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075437.1
CDS	78..9902
Translation	MKALVVVVIFSCLVAGAARAEESTEWQVEWTTEPAYNLSIIEPFTVPGYLLPDLNISDIISTVGMMYQAEDLVHAIGSLCVEMGPKLSWLSDGAMRELCDPVLEALRNGSYAEAYSICQSSWSFLETLIGGEEGGLEGEGEIDHGPIRRKRWGLSVPSIELPPLPSSPIQAVLNITVPFSPIEDLDFNWTNPIFDDTANMVKNIGLIPDLNRNWSFPIFDGTGIEVDFPYPEFSDKYGICSSLAGLISYNQPSSPSHGSIFGVVLNVIVAEQVISIPGKCLSFFDGEEGSGDIDDDPSSKHEPDTMIWAFATLGGFDDIEQICNVSSWALLMRDEGKFELFNELVNQITTSIYSVISSRSRCIEFADSLHANIGLAPGNYSHVTSSEALCDEIMNFPDWRGNMSGIDFYNYDSMDEKDAFIYLLSIVEILSHKSDMASLEFLCRMSPYADEMFCTSLAYNDYRDVSREDIIEHCLRYVAFDGFEFDFLALLNAIGAEFDFDFYDFDQTCSHLTDIVFNSVIRQQFTIDNMIQKGMKAIGRVLSPIFCDLHSFRNGSLDSLEAPCGTPGRGCFNWTRPGEDYFFDRLDEGFIVGTIFSYLGGYRDGDHICHQMAFALDDETALDMVAGKLRDSVYRLFDDVPFCSDTIGLIEKLMGVDEHAAQMIGFNSTDAFCGEISKIYHQGGNYNFSFVPLLDLFTNHSHGLEHFVTSESIMHVTTEMMKFAAMIMHSDNIGDAISKSCYMWQDSGPGITMPLFEVDDLCQVVLALNPAELQDVCIHDIIPWIEDLFDHHHDDEERGEENEIDFDFTGILETVGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGQIVSPIFCDFESFYDLPASHLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALDDDEQLDMVANKMKDSIFRFFNDPSFCPATMDIINKLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSLEPLFVLFADVEEEGDFDPAAIFNATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEVDDLCQTVLAFNSAELQDVCIHELFPWIEDIFDHHDDDEMREEENEIDFDFTGILETGGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFHDHPDTVLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALVDDAQLDMVANKMKDSIFRFFNDPSFCPATMDIINRLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSLEPLYVLFANVEEEGEFDPAAIINATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEIDDLCQLVLEDNSDAVYQVCMGELFPLIESVLEDEMYDFNDNRTNTVVDLSNIEICLDNGEVTFEALENLDILAYLLQTTHKLYNLTVFDESNICRAVTSVVESRNDIRTLISDFAVEMMSLFMPLGAEVCSCWDQVFNDLFSDSDDSEFGVNQFNEIVRIAVNSIGLDSHHELCDHLIHLKPDNMSDYAQTVIEEILGFTADPEKCACTANRVIDFILPLVNVSMEEFNNYLYQYLGYHSVDHVCSFVAQSFSGVTVDPSSINCNSQQEPTTKATTTTQSSLDNNGNSTQSSIDNTGNSTQSPIDNTGDSNETSTDDETCLFITDCAGVCNGNASLDCARECGGSAALDCAFKCGGSATTNECGWCVGGATERPSTLGFDQCGSCIGEGTAVIDCNGDCDGTAYRDACQVCVGGNTSVAEDVSDRFLDCLGICNGSFVTNDCDECVLPFSDGTIFSTADCNGDCNGFADINECSYCVGGNTGNSVDTGLSACGVCDATIEPDFCLGCDGVADSGLVTDACDVCDGDGSTCFTVDNIAPSIIPANTDYQVKLRGAGFQDATPKQCKFLDSSGEQVSVVDISDEVDDEFNCTANLPQGTYSVYLSTESTELSEAFVTLYVYSEVIINSLSQSEFDIDDSVISISVNMVSTSGAFTAYKDLTTPKAIVFTETERLIYEGTFGGDNDELLEFTGPMLTTSAQVVVYPTFNGIDYLVTPDDAGFTITYYAPAPVAQSLKFSPTGHLLELEFDVAINEDSVTSCDAVFMNTTSLGGAAAQCFAFPYLKTIYILPFSSDSGNLIEPDDELVLRADVLKKRDEEYARYASGTLTASSPDEAVTVYAALTGTEAISSCGNVRLSALQSFGNAGRFFTYQWSVTSGGSIPSGLTDDLTAAAESAYVTLDGSYLDADVPYTFSMTAENFLGGSDTKTLEVTRSALNKPDVVIYPERVDPNSALISQAFYLRAAVRFYEACGGAEQMEYAWSVDDDSIALNLQTINKPLLDVAAGSLPGDSQVTFTLEVNRESDPSTVTTQTVTITTVYTDLDALIDGGSERTIGVDSESFTLDGSSSFDPDRENEEMTYLWTCRKSNTETGSYEPCVSSETREVFPAEADSKTSTLTLTSNNLYSDSTYLFTLEINKLSRTASTSVTILSKSGNPPTISLDESLPEKIKSSSSHTLRVLISHTSDITIDWATFDIDLGYGYVDFSQLNSQSFLIQGDTSPFITYAFVTIPTGTLEEDVAYSFSVTVTDTEGQVSASKLTTSTYGGVSSCTFSLGDGSTSYQELDEITLLTQNCVTDEEAYPLSYQVFREKVESSVTRYNAMTGALAEASSTILGLPSFSDNQNTFVVKVCNDFGSCSSYSLTLDVTPIASFSQEQFDESITNLVEPQKLSGNFLDALVNFNTITSVGVDSTSRKRRAVSETSSTATEQLDLLQNAISSTVLDTATAQTLLDQSDGVVIADLTLSDMDIYLDILIELVAVFSNEGLPESSAEIVLTKTSEIAAELDPQYNSDMLAKITTLRNTLIQGQLSQIPLGGDPTETRSGSVTTRAFYAIPSDTLSTASSNTSFNVKFGSEIESLYGASWSCASGSTCSGVQIMFTQYDDEVDYYSTTDEDKANRASSILEIDLLDPSDNSELSISGLSSPISINMTASRLQSDKVYDCYWWDTSSSSWSQDGLTTVYPSSVLSECQTTHLSAFTLIAVDSPTTIMMATTIVDDASTSTDAVASTATTEISESGSSGLSSTVIIVIAVVISVVLILIIIIAIVVVMSKKSSKVGNADIENSNGNGARNGTSTAGGLAPVESANQAFNAPPTAPAHPTHTVQVVAAPAWAPASQEPIMDVAGLPGSPSPPPDGPHIDTIIKDVQNDSKAQTPMDRAMTPVE

Target ORF information:

RefSeq Version	NM_001081968.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081968.1

ORF Insert Sequence:

ATGAAGGCAC TTGTGGTGGT TGTCATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCATC 
ATCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GAATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATGG GACCCAAGCT TAGCTGGCTT TCCGATGGAG CAATGCGAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAGT 
TCTTGGTCCT TTCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCAATCGAAG ATTTGGATTT TAACTGGACC AACCCTATAT TCGATGATAC TGCGAACATG 
GTCAAGAATA TTGGACTCAT CCCGGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGAA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCGAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGAGTGG TTCTAAACGT CATCGTTGCC GAACAGGTTA TATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTCCAAA 
CACGAACCTG ATACAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGAAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATGTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAA TGAATTTCCC CGATTGGAGA 
GGCAATATGA GCGGGATTGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGACTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GCACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TGGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GACTGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAC CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTCTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
CAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AACTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGACATTGGC TTTGTTATTG GTACAATTTT CAGTTATATT 
GGAGGCTACC GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGATGATGAT 
GAACAACTTG ACATGGTAGC AAATAAAATG AAGGATAGTA TCTTCCGATT CTTCAACGAC 
CCATCATTCT GTCCTGCGAC GATGGACATT ATCAACAAGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTTTGTTTT GTTTGCCGAT 
GTTGAAGAAG AAGGAGACTT TGACCCTGCA GCCATCTTTA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGAG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGGTTGACGA TTTATGTCAA 
ACAGTATTGG CCTTTAATTC TGCAGAACTC CAAGATGTCT GCATCCACGA ACTATTTCCC 
TGGATTGAGG ACATTTTTGA CCACCATGAT GATGATGAAA TGAGGGAAGA AGAAAACGAA 
ATTGACTTTG ATTTCACAGG GATTCTCGAA ACGGGAGGCG AGGAATTCGG ATTCGACTTT 
CACGATTCTC ACCAAAGTTG CTCTCATCTT ACTGGTATTC TCTTTGATGA TACCGTCAGC 
CAAAACTTTC CAATCGACAG CGTTGTGGTA AGGGGAATAA GAGCTGTTGG TAAGATAGTA 
TCACCCATAT TCTGTGATTT TGAATCATTT CATGACCACC CAGATACTGT CTTGGAAGCT 
CCGTGTGCAA CTCCTGGACA AAACTGCTTT GATTGGTCAA GCCGAGGAGA AGACTTCTTC 
TATGAGCGGG AAGATATTGG CTTTGTCATT GGTACAATTT TCAGTTATAT TGGAGGCTAC 
AGAGATGGGG ACCACCTTTG TCACCAAATG GCCTTTGCAC TGGTTGATGA TGCACAGCTT 
GACATGGTAG CAAATAAAAT GAAGGATAGT ATCTTCCGAT TCTTCAACGA CCCATCATTC 
TGTCCTGCGA CGATGGACAT TATCAACAGG CTCATCGGAT TTGACGAGGA TGCTGCAAGT 
TTACTCGGCT TTGAGAATGC GGATGCCTTC TGCGGGAAGG TCAATCAAAT ATACAATGAA 
GATGGCGATT ATAACGTCAG TTTGGAGCCT CTCTATGTTT TGTTTGCCAA TGTTGAAGAA 
GAAGGAGAAT TTGACCCTGC AGCCATTATA AATGCCACAA CCGAGATGAT TAAATTCGCT 
GCCATGATCA TGCGTTCAGA CCACATTGGA GATGCCATTT CTAAAACATG CTACGTGTGG 
CAGGAATCTG CTGCAATGTT AAATATTTCA GAGATTGATG ATTTATGTCA ATTGGTTCTT 
GAGGACAATT CCGATGCTGT TTACCAAGTC TGCATGGGTG AACTTTTCCC ACTTATTGAG 
TCAGTCCTAG AAGATGAGAT GTATGATTTC AATGATAATC GTACAAACAC CGTTGTAGAT 
TTATCGAACA TTGAGATCTG TCTCGATAAT GGCGAGGTGA CTTTTGAGGC ACTTGAAAAT 
TTAGATATAT TGGCATATCT ACTACAAACC ACACACAAGC TCTATAATTT AACCGTGTTT 
GACGAGAGCA ACATTTGTAG AGCAGTGACC AGCGTAGTAG AATCTAGAAA TGACATTCGG 
ACACTGATAT CTGATTTTGC TGTGGAAATG ATGTCCCTCT TCATGCCGCT TGGTGCAGAA 
GTATGTTCTT GTTGGGATCA AGTTTTTAAT GATCTCTTCT CGGACTCCGA CGACTCGGAA 
TTTGGCGTAA ACCAATTCAA TGAGATAGTC CGGATTGCTG TGAACAGTAT TGGATTGGAC 
AGTCATCACG AGCTCTGTGA CCATCTCATT CATCTCAAGC CGGACAACAT GTCTGATTAC 
GCACAAACGG TGATTGAGGA AATCCTAGGT TTTACGGCTG ATCCTGAGAA GTGTGCCTGT 
ACCGCCAATC GTGTCATCGA TTTCATCCTT CCCTTGGTTA ATGTTTCAAT GGAGGAATTC 
AATAACTACC TTTACCAGTA CCTGGGATAC CATTCTGTGG ATCACGTGTG TAGTTTCGTG 
GCCCAATCCT TCTCGGGAGT AACAGTCGAC CCTTCAAGCA TAAACTGTAA CAGCCAACAA 
GAACCAACAA CCAAAGCTAC GACCACAACT CAATCATCAT TAGACAATAA TGGAAACTCA 
ACTCAATCAT CAATAGACAA TACTGGAAAC TCAACTCAAT CACCAATAGA CAATACTGGA 
GACTCGAATG AAACTTCGAC TGACGACGAA ACCTGTCTCT TCATCACTGA CTGCGCCGGT 
GTATGCAACG GTAACGCTAG TTTGGATTGC GCCCGAGAAT GCGGCGGCAG CGCGGCACTC 
GACTGCGCCT TCAAGTGCGG TGGCTCTGCG ACCACGAACG AATGTGGATG GTGCGTCGGT 
GGAGCTACGG AAAGACCAAG CACTTTAGGT TTCGACCAGT GTGGTTCCTG CATTGGGGAA 
GGCACTGCGG TCATCGACTG CAATGGTGAC TGTGACGGTA CTGCTTACCG CGATGCCTGT 
CAAGTCTGCG TAGGTGGCAA TACGAGTGTG GCTGAGGATG TAAGCGATCG TTTCTTGGAC 
TGCCTAGGTA TCTGTAATGG TAGTTTTGTG ACCAATGATT GTGATGAATG CGTGCTGCCA 
TTCAGCGATG GGACTATTTT CTCCACGGCG GATTGTAACG GTGACTGCAA TGGATTTGCC 
GACATAAATG AGTGTAGTTA CTGTGTTGGG GGTAATACCG GTAACTCTGT GGATACTGGA 
TTGTCTGCAT GTGGAGTCTG CGATGCAACG ATCGAACCAG ACTTCTGCCT CGGCTGTGAT 
GGCGTCGCTG ATAGTGGCCT AGTGACCGAT GCGTGTGATG TATGCGATGG GGATGGTAGC 
ACTTGTTTCA CCGTGGACAA TATAGCTCCC TCTATCATCC CAGCTAACAC CGACTATCAG 
GTTAAACTTC GTGGTGCCGG ATTCCAAGAT GCGACTCCCA AACAGTGTAA GTTCCTGGAT 
AGCAGCGGTG AGCAGGTCAG CGTGGTCGAT ATCAGCGATG AGGTGGATGA CGAGTTCAAC 
TGCACCGCTA ATCTTCCTCA AGGAACGTAT TCTGTTTACC TTTCGACGGA GTCAACGGAA 
CTATCAGAAG CATTCGTCAC CCTCTATGTC TATTCAGAAG TTATAATAAA CAGCTTATCC 
CAGAGCGAGT TTGATATAGA TGATAGTGTG ATTTCCATCT CTGTCAACAT GGTATCAACA 
AGCGGAGCCT TTACGGCATA CAAGGACTTG ACCACTCCAA AAGCTATCGT CTTCACAGAG 
ACCGAGAGAC TGATCTACGA AGGAACTTTT GGAGGAGACA ACGACGAGCT TCTCGAGTTT 
ACTGGTCCGA TGCTAACTAC CTCGGCTCAG GTTGTCGTCT ACCCGACGTT CAACGGAATC 
GACTACCTGG TAACTCCCGA TGATGCCGGT TTCACGATCA CCTACTACGC CCCTGCACCG 
GTCGCTCAGA GTCTGAAATT CTCCCCTACT GGACATCTTC TGGAACTCGA GTTCGACGTC 
GCAATCAACG AAGACTCAGT GACATCATGT GATGCAGTAT TCATGAATAC CACCAGCCTG 
GGTGGTGCTG CTGCCCAGTG CTTTGCATTT CCTTACCTAA AAACTATCTA TATACTACCC 
TTCAGTTCGG ACAGTGGAAA TCTTATTGAA CCAGATGATG AGCTAGTGCT GCGCGCTGAC 
GTTCTGAAAA AACGTGATGA GGAATACGCT CGGTACGCCA GCGGTACTCT AACCGCATCC 
TCCCCCGACG AGGCGGTTAC CGTCTACGCG GCTCTGACCG GTACAGAAGC AATTAGCAGC 
TGCGGTAATG TACGACTTAG TGCTCTGCAA TCCTTTGGAA ACGCAGGACG TTTTTTCACC 
TATCAGTGGT CTGTGACCAG TGGCGGTTCG ATCCCATCTG GCTTGACGGA TGATCTAACA 
GCCGCAGCGG AGTCCGCCTA TGTCACTCTC GATGGATCCT ACCTCGACGC GGACGTGCCG 
TATACATTCA GCATGACAGC CGAGAACTTC CTTGGAGGCA GTGACACAAA GACCTTGGAG 
GTGACTCGGT CTGCTCTCAA CAAACCAGAC GTCGTGATCT ACCCGGAAAG AGTTGATCCT 
AATAGTGCCC TTATCTCGCA AGCGTTCTAC CTCCGCGCTG CTGTGCGATT CTACGAGGCC 
TGCGGGGGAG CTGAGCAGAT GGAGTATGCC TGGTCTGTAG ATGATGATAG TATTGCTCTT 
AACCTACAAA CCATAAACAA ACCACTCCTG GATGTGGCAG CTGGTTCTTT ACCAGGCGAC 
TCCCAAGTGA CCTTTACACT GGAGGTCAAC CGGGAAAGTG ACCCCAGCAC GGTGACCACG 
CAGACCGTGA CCATTACCAC GGTCTACACC GACCTCGACG CCCTAATCGA TGGCGGGAGT 
GAGCGGACCA TCGGCGTCGA CAGTGAGTCA TTCACACTGG ATGGATCGTC GTCGTTTGAT 
CCCGACAGGG AAAATGAAGA GATGACCTAC CTCTGGACTT GTCGGAAGTC GAACACTGAG 
ACAGGCAGCT ACGAGCCTTG TGTATCAAGC GAGACACGTG AGGTGTTCCC GGCGGAAGCT 
GACTCCAAAA CAAGTACTCT CACGTTAACT TCCAACAATC TTTACTCGGA CTCCACCTAT 
CTGTTCACTC TGGAGATCAA CAAACTGTCA AGGACAGCAT CGACCAGTGT CACCATCCTT 
TCCAAGTCTG GCAATCCTCC AACGATCAGT CTCGATGAAT CCCTTCCTGA GAAGATAAAG 
AGCAGTAGTT CTCATACGCT ACGAGTATTG ATCAGCCATA CATCAGATAT TACCATTGAC 
TGGGCTACTT TCGACATAGA TCTTGGATAT GGATACGTTG ACTTTTCACA GCTGAATAGT 
CAGTCATTCC TTATACAAGG AGATACATCA CCCTTCATCA CATACGCCTT TGTAACTATC 
CCCACAGGAA CTTTGGAAGA GGATGTTGCC TACTCTTTCT CAGTGACGGT AACTGATACC 
GAAGGACAGG TGTCAGCTAG TAAATTGACT ACCAGCACGT ATGGAGGAGT ATCATCCTGT 
ACATTCAGTT TGGGTGATGG TTCAACCAGC TATCAAGAAC TTGACGAGAT CACTCTCCTA 
ACTCAGAACT GCGTGACTGA TGAAGAAGCT TACCCTCTGT CTTACCAAGT GTTCCGTGAG 
AAGGTCGAAT CATCTGTCAC CCGTTACAAC GCGATGACGG GTGCATTGGC AGAAGCGAGC 
AGCACTATAT TGGGTCTGCC GTCCTTTAGC GACAACCAGA ACACATTTGT TGTCAAGGTG 
TGCAATGATT TTGGAAGCTG TTCCTCGTAC AGTCTTACAT TGGACGTCAC TCCCATCGCA 
TCTTTCAGTC AAGAACAGTT TGATGAAAGT ATAACCAATC TGGTAGAACC ACAAAAGCTC 
TCAGGGAATT TCCTAGATGC GCTGGTAAAC TTCAACACCA TCACGTCAGT GGGTGTCGAC 
AGCACGAGTA GGAAACGGAG AGCAGTCTCG GAGACATCCA GCACAGCTAC TGAGCAGCTG 
GATTTACTTC AGAATGCCAT CTCGTCGACG GTCCTTGACA CGGCCACCGC CCAGACTCTG 
CTTGATCAGT CGGATGGCGT GGTCATTGCT GATCTCACAC TGTCTGATAT GGACATCTAC 
CTGGATATTT TAATTGAGCT CGTAGCTGTT TTCAGTAATG AAGGGCTTCC AGAGTCTAGC 
GCCGAGATCG TTCTTACAAA GACAAGCGAG ATCGCTGCTG AACTCGACCC TCAATACAAC 
TCGGACATGC TTGCCAAAAT TACGACCCTG AGAAACACAT TAATCCAGGG CCAGCTGAGT 
CAGATCCCAC TTGGAGGGGA CCCTACGGAG ACCAGGTCTG GTTCCGTTAC CACCAGGGCC 
TTCTATGCTA TCCCTAGTGA TACCCTCAGT ACTGCATCCA GCAACACCTC CTTCAACGTC 
AAGTTCGGAA GCGAGATCGA AAGCTTATAT GGTGCAAGTT GGTCGTGTGC TTCTGGAAGC 
ACTTGTTCTG GTGTTCAGAT CATGTTTACT CAGTACGACG ACGAGGTTGA TTACTACTCG 
ACCACGGACG AGGATAAGGC GAACAGAGCA TCTTCTATCC TTGAAATCGA TCTGCTTGAC 
CCGAGTGACA ATTCTGAGTT GAGTATTTCT GGACTGAGCA GCCCCATATC AATCAACATG 
ACCGCTTCAC GCCTCCAATC CGACAAAGTC TATGACTGCT ATTGGTGGGA CACTAGCAGC 
AGCTCTTGGA GCCAGGATGG ACTTACGACC GTCTACCCCT CTTCGGTCTT GTCTGAGTGC 
CAAACTACTC ATCTTTCAGC CTTCACCCTG ATCGCTGTTG ATTCTCCTAC TACGATTATG 
ATGGCTACCA CGATAGTTGA TGACGCCAGT ACTTCTACCG ATGCAGTAGC CTCGACTGCG 
ACAACGGAAA TCAGTGAGAG CGGCAGCAGT GGTCTAAGTT CTACCGTCAT CATCGTCATT 
GCCGTGGTCA TATCTGTCGT TTTGATCCTA ATTATTATCA TCGCCATCGT GGTCGTGATG 
TCGAAGAAGT CCTCCAAGGT CGGTAATGCT GATATCGAGA ACTCGAACGG CAATGGTGCC 
AGGAATGGGA CCAGCACCGC CGGTGGACTC GCTCCTGTTG AATCTGCGAA CCAGGCATTC 
AATGCCCCAC CAACAGCACC GGCCCACCCA ACCCATACTG TTCAAGTGGT CGCTGCCCCA 
GCTTGGGCAC CTGCTTCTCA AGAGCCTATC ATGGACGTTG CAGGCCTTCC TGGATCCCCA 
TCACCCCCTC CTGATGGACC CCATATTGAT ACCATCATAA AGGATGTTCA GAATGATAGT 
AAGGCCCAAA CTCCAATGGA TAGAGCCATG ACGCCCGTTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REJ8 ( XM_011673693.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011671995.1 Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X1, mRNA.

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CloneID	OSd30107
Clone ID Related Accession (Same CDS sequence)	XM_011673693.1
Accession Version	XM_011673693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	receptor for egg jelly 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011984579.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC TTGTGGTGGT TGTCATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCATC 
ATCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATGG GACCCAAGCT TAGCTGGCTT ACCGGTGGAG CAATGCGAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAGT 
TCTTGGTCCT TCCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCAATCGAAA ATTTGGATTT TAACTGGACC AACTCTTTAT TCGATGATAC TGCGAATATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGAA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGATTGG TTCTAAACGT CATCGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG ATACAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGAAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATGTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAA TGAATTTCCC CGATTGGAGA 
GGCAATATGA GCGGGATTGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGACTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GAATGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AACTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGATTGATGA TTTATGTCAA 
TTGGTTCTTG AGGACAATTC CGATGCTGTT TACCAAGTCT GCATGGGTGA ACTTTTCCCA 
CTTATTGAGT CAATTCTAGA AGACGAGATG TATGATTTCA ATGATAATCA TACAAACACC 
GTTGTAGATT TATCGAACAT TGAGATCTGT CTCGATAATG GCGAGGTGAC TTTTGAGGCA 
CTTGAAAATT TAGATATATT GGCATATCTA CTACAAACCA CACACAAGCT CTATAATTTA 
ACCGTGTTCG ACGAGAGCAA CATTTGTAGA GCAGTGACCA GCGTAGTAGA ATCTAGAAAT 
GACATTCCGA CACTGATATC TGAATTTGCT GTGGAAATGA TGTCCCTCTT CATGCCGCTT 
GGTGCAGAAA TATGCTCTTG TTGGGATCAA GTTTTTGATG ATCTCTTCTC GGACTCCGAC 
GACTCGGAAT TTGGCGTAAA CCAATTCAAT GAGATAGTCC GGATTGCTGT GAACAGTATT 
GGATTAGACA GTCGTCATGA ACTCTGTGAC CATCTCATTC ATCTAAAGCC GGACAACATG 
TCTGATTACG CACAAACGGT GATTGAGGAA ATCCTTGGTT TTACGGCTGA TCCTGAGAAG 
TGTGCCTGTA CCGCCAATCG TGTCATCGAT TTCATCCTTC CCTTGGTTAA TGTTTCAATG 
GAGGAAATCA ATAACTACCT TTACCAGTAC CTGGGATACC ATTCTGTGGA TCACGTGTGT 
AGTTTCGTGG CCCAATCCTT CTCGGGAGTA ATAGTCGACC CTTCAAGCAT AAACTGTAAC 
AGCCAACAAG AACCAACAAC CAAAGCTACG ACCACAACTC AATCATCATT AGACAATAAT 
GGAAACTCAA CTCAATCATC AATAGACAAT ACTGGAAACT CAACTCAATC ACCAATAGAC 
AATACTGGAG ACTCGAATGA AACTTCGACT GACGACGAAA CCTGTCTCTT CATCACTGAC 
TGCGCCGGTG TATGCAACGG TAACGCTAGT TTGGATTGCG CCCGAGAATG CGGCGGCAGC 
GCGGCACTCG ACTGCGCCTT CAAGTGCGGT GGCTCTGCGA CCACGAACGA ATGTGGATGG 
TGCGTCGGTG GAGCTACGGA AAGACCAAGC ACTTTAGGTT TCGACCAGTG TGGTTCCTGC 
ATTGGGGAAG GCACTGCGGT CATCGACTGC AATGGTGACT GTGACGGTAC TGCTTACCGC 
GATGCCTGTC AAGTCTGCGT AGGTGGCAAT ACGAGTGTGG CTGAGGATGT AAGCGATCGT 
TTCTTGGACT GCCTAGGTAT CTGTAATGGT AGTTTTGTGA CCAATGATTG TGATGAATGC 
GTGCTGCCAT TCAGCGATGG GACTATTTTC TCCACGGCGG ATTGTAACGG TGACTGCAAT 
GGATTTGCCG ACATAAATGA GTGTAGTTAC TGTGTTGGGG GTAATACCGG TAACTCTGTG 
GATACTGGAT TGTCTGCATG TGGAGTCTGC AATACTACGA TCGAACCAGA CTTCTGCCTC 
GGCTGTGATG GCGTCGCTGA TAGTGGCCTA GTGACCGATG CGTGTGATGT ATGCGATGGG 
GATGGTAGCA CTTGTTTCAC CGTGGACAAT ATAGCTCCCT CTATCATCCC AGCTAACACC 
GACTATCAGG TTAAACTTCG TGGTGCCGGA TTCCAAGATG CGACTACCAA ACAGTGTAAG 
TTCCTGGATA GCAGCGGTGA GCAGGTCAGC GTGGTCGATA TCAGCGATGA GGTGGATGAC 
GAGTTCAACT GCACCGCTAA TCTTCCTCAA GGAACGTATT CTGTTTACCT TTCGACGGAG 
TCAACGGAAC TATCAGAAGC ATTCGTCACC CTCTATGTCT ATTCAGAAGT TATAATAAAC 
AGCTTATCCC AGAGCGAGTT TGATATAGAT GATAGTGTGA TTTCCATCTC TGTCAACATG 
GTATCAACAA GCGGAGCCTT TACGGCATAC AAGGACTTGA CCACTCCAAA AGCTATCGTC 
TTCACAGAGA CCGAGAGACT TTACTACGAA GGAACTTTTG GAGGAGACAA CGACGAGCTT 
CTCGAGTTTA CGGGTCCGAT GCTGACTACC TCGGCTCAAG TTGTCGTTTA CCCGTCCTTC 
AACGGAATCG ACTACCTGGT CACCCCCGAT GATGCTGGTT TCACGATCAC CTACTACGCC 
CCTGCACCGG TCGCTCAGAG TCTTAAATTC TCCCCTACTG GACATCTTCT GGAACTCGAG 
TTCGACGTCG CAATCAACGA AGACTCGGTG ACATCATGTG ATGCAGTATT CATGAATACC 
ACCAGCCTGG GTGGTGCTGC TGCCCAGTGC TTTGCATTCC CTTACCTAAA AACTATCTAT 
ATACTACCCT TCAGTTCGGC CAGTGTAAAT CTTATTGGAC CAGATGATGA GCTAGTGCTG 
CGCGCTGACG TTCTGAAAAA ACGTGATGAG GAATACGCTC GGTACGCCAG CGGTACTCTT 
ACCGCATCCT CCCCCGACGA GGCGGTAACC GTCTACGCGG CTCTGACCGG TACAGAAGCA 
ATTAGCAGCT GCGGTAATGT ACGACTTAGT GCTCTGCAAT CCTTTGGAAA CGCAGGACGT 
TTTTTCACCT ATCAGTGGTC TGTGACCAGT GGCGGTTCGA TCCCATCTGG CTTGACGGAT 
GATCTAACAG CCGCAGCGGA GTCCGCCTAT GTCACTCTCG ATGGATCCTA CCTCGACGCG 
GACGTGCCGT ATACATTCAG CATGACAGCA GAGAACTTCC TCGGAGGCAG TGACACAAAG 
ACCTTGGAGG TGACTCGGTC TGCTCTCAAC AAACCAGACG TCGTGATCTA CCCGGAAAGA 
GTTGATCCTA ATAGTGCCCT TATCTCGCAA GCGTTCTACC TCCGCGCTGC TGTACGATTC 
TACGAGGCCT GCGGTGGAGC TGAGCAGATG GAGTATGCCT GGTCTGTAGA TGATGATAGT 
ATTGCTCTTA ACCTACAAAC CATAAACAAA CCACTCCTGT ATGTGGCAGC TGGTTCTTTA 
CCAGGCGACT CCCAAGTGAC CTTCACACTG GAGGTCAACC GGGAAAGTGA TCCCAGCACG 
GTGACCACGC AGACCGTGAC CATTACCACG GTCTACACCG ACCTCGACGC CCTAATCGAT 
GGCGGGAGTG AGCGGACCAT CGGCGTCGAC AGTGAGTCGT TCACACTGGA TGGATCGTCG 
TCGTTTGATC CCGACAGTGA AAATGAAGAG ATGACCTACC TCTGGACTTG TCAGAAGTCA 
AACACTGAGA CAGGCAGCTA CGAGCCTTGT GTATCAAGCG AGACACGTGA GGTGTTCCCG 
GCGGAAGCTG ACTCCAAAAC AAGTACTCTC ACGTTAGCTT CCAACAATCT TTACTCGGAC 
TCCACCTATC TGTTCACTCT GGAGATCAAC AAACTGTCAA GGACAGCATC GACCAGTGTC 
ACCATCCTTT CCAAGTCTGG CAATCCTCCA ACGATCAGTC TCGATGAATC CCTTCCTGAG 
AAGATAAAGA GCAGTAGTTC TCATACGCTA CGAGTATTGA TCAGCCATAC ATCAGATATT 
ACCATTGACT GGGCTACTGT CGACATAGAT CTTGGATATG GATACGTTGA CTTTTCACAG 
CTGAATAGTC AGTCATTCCT TATACAAGGA GATACATCAC CCTTCATCAC ATACGCCTTT 
GTAACTATCC CCACAGGAAC TTTGGAAGAG GATGTTGCCT ACTCTTTCTC AGTGACGGTA 
ACTGATACCG AAGGACAGGT GTCAGCTAGT AAATTGACTA CCAACACGTA TGGAGGAGTA 
TCATCCTGTA CATTCAGTTT GGGTGATGGT TCAACCAGCT ATCAAGAACT TGACGAGATC 
ACTCTCCTAA CTCAGAACTG CGTGACTGAT GAAGAAGCTT ACCCTCTGTC TTACCAAGTG 
TTCCGTGAGA AGGTCGAATC ATCTGTCACC CGTTACAACG CGATGACGGG TGCATTGGCA 
GAAGCGAGCA GCACTATATT GGGTCTGCCG TCCTTTAGCG ACAACCAGAA CACATTTGTT 
GTCAAGGTGT GCAATGATTT TGGAAGCTGT TCCTCGTACA GTCTTACATT GGACGTCACT 
CCCATCGCAT CTTTCAGTCA AGAACAGTTT GATGAAAGTA TAACCAATCT GGTAGAACCA 
CAAAAGCTCT CAGGGAATTT CCTAGATGCG CTGGTAAACT TCAACACCAT CACGTCAGTG 
GGTGTCGACA GCACGAGTAG GAAACGGAGA GCAGTCTCGG AGACATCCAG CACAGCTACT 
GAGCAGCTGG ATTTACTTCA GAATGCCATC TCGTCGACGG TCCTTGACAC GGCCACCGCC 
CAGACTCTGC TTGATCAGTC GGATGGCGTG GTCATTGCTG ATCTCACACT GTCTGATATG 
GACATCTACC TGGATATTTT AATTGAGCTC GTAGCTGTTT TCAGTAATGA AGGGCTTCCA 
GAGTCTAGCG CCGAGATCGT TCTTACAAAG ACAAGCGAGA TCGCTGCTGA ACTCGACCCT 
CAATACAACT CGGACATGCT TGCCAAAATT ACGACCCTGA GAAACACATT AATCCAGGGC 
CAGCTGAGTC AGATCCCACT TGGAGGGGAC CCTACGGAGA CCAGGTCTGG TTCCGTTACC 
ACCAGGGCCT TCTATGCTAT CCCTAGTGAT ACCCTCAGTA CTGCATCCAG CAACACCTCC 
TTCAACGTCA AGTTCGGAAG CGAGATCGAA AGCTTATATG GTGCAAGTTG GTCGTGTGCT 
TCTGGAAGCA CTTGTTCTGG TGTTCAGATC ATGTTTACTC AGTACGACGA CGAGGTTGAT 
TACTACTCGA CTACGGACGA GGATAAGGCG AACAGAGCAT CTTCTATCCT TGAAATCGAT 
CTGCTTGACC CGAGTGACAA TTCTGAGTTG AGTATTACTG GACTGAGCAG CCCCATATCA 
ATCAACATGA CCGCTTCACG CCTCCAATCC GACAAAGTCT ATGACTGCTA TTGGTGGGAC 
ACTAGCAGCA GCTCTTGGAG CCAGGATGGA CTTACGACCG TCTACCACTC ATCGGTCTTG 
TCTGAGTGCC AAACTACTCA TCTTTCAGCC TTCACCCTGA TCGCTGTTGA TTCTCCTACT 
ACGATTATGA TGGCTACCAC GATAGTTGAT GACGCCAGTA CTTCTACCGA TGCAGTAGCC 
TCGACTGCGA CAACGGAAAT CAGTGAGAGC GGCAGCAGTG GTCTAAGTTC TACCGTCATC 
ATCGTCATTG CCGTGGTCAT ATCTGTCGTT TTGATCCTAA TTATTATCAT CGCCATCGTG 
GTCGTGATGT CGAAGAAGTC CTCCAAGGTC GGTAATGCTG ATATCGAGAA CTCGAACGGC 
AATGGTGCCA GGAATGGGAC CAGCACCGCC GATGGACTCG CTCCTGTTGA ATCTGCGAAC 
CAGGCATTCA ATGCCCCACC AACAGCACCG GCCCACCCAA CCCATACTGT TCAAGTGGTC 
GCTGCCCCAG CTTCGGCACC TGCTTCTCAA GAGCCTATCA TGGACGTTGC AGACCTTCCT 
GGATCCCCAT CACCCCCTCC TGATGGACCC CATATTGATA CCATCATAAA GGATGTTCAG 
AATGATAGTA GGGCCCAAAC TCCAATGGAT AGAGCCATGA CGCCCGTTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011671995.1
CDS	198..9071
Translation	MKALVVVVIFSCLVAGAARAEESTEWQVEWTTEPAYNLSIIEPFTVPGYLLPDLNISDIISTVGMMYQAEDLVHAIGSLCVEMGPKLSWLTGGAMRELCDPVLEALRNGSYAEAYSICQSSWSFLETLIGGEEGGLEGEGEIDHGPIRRKRWGLSVPSIELPPLPSSPIQAVLNITVPFSPIENLDFNWTNSLFDDTANMVENIGLIPDLNRNWSFPIFDGTGIEVDFPYPEFSDKYGICSSLAGLISYNQPSSPSHGSIFGLVLNVIVAEQVRSIPGKCLSFFDGEEGSGDIDDDPSFKHEPDTMIWAFATLGGFDDIEQICNVSSWALLMRDEGKFELFNELVNQITTSIYSVISSRSRCIEFADSLHANIGLAPGNYSHVTSSEALCDEIMNFPDWRGNMSGIDFYNYDSMDEKDAFIYLLSIVEILSHKSDMASLEFLCRMSPYADEMFCTSLAYNDYRDVSREDIIEHCLRYVAFDGFEFDFLALLNAIGAEFDFDFYDFDQTCSHLTDIVFNSVIRQQFTIDNMIQKGMKAIGRVLSPIFCDLHSFRNGSLDSLEAPCGTPGRGCFNWTRPGEDYFFDRLDEGFIVGTIFSYLGGYRDGDHICHQMAFALDDETVLDMVAGKLRDSVYRLFDDVPFCSDTIGLIEKLIGVDEHAAQMIGFNSTDAFCGEISKIYHQGGNYNFSFVPLLDLFTNHSHGLEHFVTSESIMHVTNEMMKFAAMIMHSDNIGDAISKSCYMWQDSGPGITMPLFEVDDLCQVVLALNPAELQDVCIHDIIPWIEDLFDHHHDDEERGEENEIDFDFTGILETVGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFYDLPASHLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALEDDAQLDMVANKMKDSIFRFFNDPSFCPVTMDLINRLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSLEPLYVLFANVEEEGEFDPAAIINATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEIDDLCQLVLEDNSDAVYQVCMGELFPLIESILEDEMYDFNDNHTNTVVDLSNIEICLDNGEVTFEALENLDILAYLLQTTHKLYNLTVFDESNICRAVTSVVESRNDIPTLISEFAVEMMSLFMPLGAEICSCWDQVFDDLFSDSDDSEFGVNQFNEIVRIAVNSIGLDSRHELCDHLIHLKPDNMSDYAQTVIEEILGFTADPEKCACTANRVIDFILPLVNVSMEEINNYLYQYLGYHSVDHVCSFVAQSFSGVIVDPSSINCNSQQEPTTKATTTTQSSLDNNGNSTQSSIDNTGNSTQSPIDNTGDSNETSTDDETCLFITDCAGVCNGNASLDCARECGGSAALDCAFKCGGSATTNECGWCVGGATERPSTLGFDQCGSCIGEGTAVIDCNGDCDGTAYRDACQVCVGGNTSVAEDVSDRFLDCLGICNGSFVTNDCDECVLPFSDGTIFSTADCNGDCNGFADINECSYCVGGNTGNSVDTGLSACGVCNTTIEPDFCLGCDGVADSGLVTDACDVCDGDGSTCFTVDNIAPSIIPANTDYQVKLRGAGFQDATTKQCKFLDSSGEQVSVVDISDEVDDEFNCTANLPQGTYSVYLSTESTELSEAFVTLYVYSEVIINSLSQSEFDIDDSVISISVNMVSTSGAFTAYKDLTTPKAIVFTETERLYYEGTFGGDNDELLEFTGPMLTTSAQVVVYPSFNGIDYLVTPDDAGFTITYYAPAPVAQSLKFSPTGHLLELEFDVAINEDSVTSCDAVFMNTTSLGGAAAQCFAFPYLKTIYILPFSSASVNLIGPDDELVLRADVLKKRDEEYARYASGTLTASSPDEAVTVYAALTGTEAISSCGNVRLSALQSFGNAGRFFTYQWSVTSGGSIPSGLTDDLTAAAESAYVTLDGSYLDADVPYTFSMTAENFLGGSDTKTLEVTRSALNKPDVVIYPERVDPNSALISQAFYLRAAVRFYEACGGAEQMEYAWSVDDDSIALNLQTINKPLLYVAAGSLPGDSQVTFTLEVNRESDPSTVTTQTVTITTVYTDLDALIDGGSERTIGVDSESFTLDGSSSFDPDSENEEMTYLWTCQKSNTETGSYEPCVSSETREVFPAEADSKTSTLTLASNNLYSDSTYLFTLEINKLSRTASTSVTILSKSGNPPTISLDESLPEKIKSSSSHTLRVLISHTSDITIDWATVDIDLGYGYVDFSQLNSQSFLIQGDTSPFITYAFVTIPTGTLEEDVAYSFSVTVTDTEGQVSASKLTTNTYGGVSSCTFSLGDGSTSYQELDEITLLTQNCVTDEEAYPLSYQVFREKVESSVTRYNAMTGALAEASSTILGLPSFSDNQNTFVVKVCNDFGSCSSYSLTLDVTPIASFSQEQFDESITNLVEPQKLSGNFLDALVNFNTITSVGVDSTSRKRRAVSETSSTATEQLDLLQNAISSTVLDTATAQTLLDQSDGVVIADLTLSDMDIYLDILIELVAVFSNEGLPESSAEIVLTKTSEIAAELDPQYNSDMLAKITTLRNTLIQGQLSQIPLGGDPTETRSGSVTTRAFYAIPSDTLSTASSNTSFNVKFGSEIESLYGASWSCASGSTCSGVQIMFTQYDDEVDYYSTTDEDKANRASSILEIDLLDPSDNSELSITGLSSPISINMTASRLQSDKVYDCYWWDTSSSSWSQDGLTTVYHSSVLSECQTTHLSAFTLIAVDSPTTIMMATTIVDDASTSTDAVASTATTEISESGSSGLSSTVIIVIAVVISVVLILIIIIAIVVVMSKKSSKVGNADIENSNGNGARNGTSTADGLAPVESANQAFNAPPTAPAHPTHTVQVVAAPASAPASQEPIMDVADLPGSPSPPPDGPHIDTIIKDVQNDSRAQTPMDRAMTPVE

Target ORF information:

RefSeq Version	XM_011673693.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011673693.1

ORF Insert Sequence:

ATGAAGGCAC TTGTGGTGGT TGTCATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCATC 
ATCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATGG GACCCAAGCT TAGCTGGCTT ACCGGTGGAG CAATGCGAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAGT 
TCTTGGTCCT TCCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCAATCGAAA ATTTGGATTT TAACTGGACC AACTCTTTAT TCGATGATAC TGCGAATATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGAA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGATTGG TTCTAAACGT CATCGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG ATACAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGAAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATGTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAA TGAATTTCCC CGATTGGAGA 
GGCAATATGA GCGGGATTGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGACTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GAATGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AACTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGATTGATGA TTTATGTCAA 
TTGGTTCTTG AGGACAATTC CGATGCTGTT TACCAAGTCT GCATGGGTGA ACTTTTCCCA 
CTTATTGAGT CAATTCTAGA AGACGAGATG TATGATTTCA ATGATAATCA TACAAACACC 
GTTGTAGATT TATCGAACAT TGAGATCTGT CTCGATAATG GCGAGGTGAC TTTTGAGGCA 
CTTGAAAATT TAGATATATT GGCATATCTA CTACAAACCA CACACAAGCT CTATAATTTA 
ACCGTGTTCG ACGAGAGCAA CATTTGTAGA GCAGTGACCA GCGTAGTAGA ATCTAGAAAT 
GACATTCCGA CACTGATATC TGAATTTGCT GTGGAAATGA TGTCCCTCTT CATGCCGCTT 
GGTGCAGAAA TATGCTCTTG TTGGGATCAA GTTTTTGATG ATCTCTTCTC GGACTCCGAC 
GACTCGGAAT TTGGCGTAAA CCAATTCAAT GAGATAGTCC GGATTGCTGT GAACAGTATT 
GGATTAGACA GTCGTCATGA ACTCTGTGAC CATCTCATTC ATCTAAAGCC GGACAACATG 
TCTGATTACG CACAAACGGT GATTGAGGAA ATCCTTGGTT TTACGGCTGA TCCTGAGAAG 
TGTGCCTGTA CCGCCAATCG TGTCATCGAT TTCATCCTTC CCTTGGTTAA TGTTTCAATG 
GAGGAAATCA ATAACTACCT TTACCAGTAC CTGGGATACC ATTCTGTGGA TCACGTGTGT 
AGTTTCGTGG CCCAATCCTT CTCGGGAGTA ATAGTCGACC CTTCAAGCAT AAACTGTAAC 
AGCCAACAAG AACCAACAAC CAAAGCTACG ACCACAACTC AATCATCATT AGACAATAAT 
GGAAACTCAA CTCAATCATC AATAGACAAT ACTGGAAACT CAACTCAATC ACCAATAGAC 
AATACTGGAG ACTCGAATGA AACTTCGACT GACGACGAAA CCTGTCTCTT CATCACTGAC 
TGCGCCGGTG TATGCAACGG TAACGCTAGT TTGGATTGCG CCCGAGAATG CGGCGGCAGC 
GCGGCACTCG ACTGCGCCTT CAAGTGCGGT GGCTCTGCGA CCACGAACGA ATGTGGATGG 
TGCGTCGGTG GAGCTACGGA AAGACCAAGC ACTTTAGGTT TCGACCAGTG TGGTTCCTGC 
ATTGGGGAAG GCACTGCGGT CATCGACTGC AATGGTGACT GTGACGGTAC TGCTTACCGC 
GATGCCTGTC AAGTCTGCGT AGGTGGCAAT ACGAGTGTGG CTGAGGATGT AAGCGATCGT 
TTCTTGGACT GCCTAGGTAT CTGTAATGGT AGTTTTGTGA CCAATGATTG TGATGAATGC 
GTGCTGCCAT TCAGCGATGG GACTATTTTC TCCACGGCGG ATTGTAACGG TGACTGCAAT 
GGATTTGCCG ACATAAATGA GTGTAGTTAC TGTGTTGGGG GTAATACCGG TAACTCTGTG 
GATACTGGAT TGTCTGCATG TGGAGTCTGC AATACTACGA TCGAACCAGA CTTCTGCCTC 
GGCTGTGATG GCGTCGCTGA TAGTGGCCTA GTGACCGATG CGTGTGATGT ATGCGATGGG 
GATGGTAGCA CTTGTTTCAC CGTGGACAAT ATAGCTCCCT CTATCATCCC AGCTAACACC 
GACTATCAGG TTAAACTTCG TGGTGCCGGA TTCCAAGATG CGACTACCAA ACAGTGTAAG 
TTCCTGGATA GCAGCGGTGA GCAGGTCAGC GTGGTCGATA TCAGCGATGA GGTGGATGAC 
GAGTTCAACT GCACCGCTAA TCTTCCTCAA GGAACGTATT CTGTTTACCT TTCGACGGAG 
TCAACGGAAC TATCAGAAGC ATTCGTCACC CTCTATGTCT ATTCAGAAGT TATAATAAAC 
AGCTTATCCC AGAGCGAGTT TGATATAGAT GATAGTGTGA TTTCCATCTC TGTCAACATG 
GTATCAACAA GCGGAGCCTT TACGGCATAC AAGGACTTGA CCACTCCAAA AGCTATCGTC 
TTCACAGAGA CCGAGAGACT TTACTACGAA GGAACTTTTG GAGGAGACAA CGACGAGCTT 
CTCGAGTTTA CGGGTCCGAT GCTGACTACC TCGGCTCAAG TTGTCGTTTA CCCGTCCTTC 
AACGGAATCG ACTACCTGGT CACCCCCGAT GATGCTGGTT TCACGATCAC CTACTACGCC 
CCTGCACCGG TCGCTCAGAG TCTTAAATTC TCCCCTACTG GACATCTTCT GGAACTCGAG 
TTCGACGTCG CAATCAACGA AGACTCGGTG ACATCATGTG ATGCAGTATT CATGAATACC 
ACCAGCCTGG GTGGTGCTGC TGCCCAGTGC TTTGCATTCC CTTACCTAAA AACTATCTAT 
ATACTACCCT TCAGTTCGGC CAGTGTAAAT CTTATTGGAC CAGATGATGA GCTAGTGCTG 
CGCGCTGACG TTCTGAAAAA ACGTGATGAG GAATACGCTC GGTACGCCAG CGGTACTCTT 
ACCGCATCCT CCCCCGACGA GGCGGTAACC GTCTACGCGG CTCTGACCGG TACAGAAGCA 
ATTAGCAGCT GCGGTAATGT ACGACTTAGT GCTCTGCAAT CCTTTGGAAA CGCAGGACGT 
TTTTTCACCT ATCAGTGGTC TGTGACCAGT GGCGGTTCGA TCCCATCTGG CTTGACGGAT 
GATCTAACAG CCGCAGCGGA GTCCGCCTAT GTCACTCTCG ATGGATCCTA CCTCGACGCG 
GACGTGCCGT ATACATTCAG CATGACAGCA GAGAACTTCC TCGGAGGCAG TGACACAAAG 
ACCTTGGAGG TGACTCGGTC TGCTCTCAAC AAACCAGACG TCGTGATCTA CCCGGAAAGA 
GTTGATCCTA ATAGTGCCCT TATCTCGCAA GCGTTCTACC TCCGCGCTGC TGTACGATTC 
TACGAGGCCT GCGGTGGAGC TGAGCAGATG GAGTATGCCT GGTCTGTAGA TGATGATAGT 
ATTGCTCTTA ACCTACAAAC CATAAACAAA CCACTCCTGT ATGTGGCAGC TGGTTCTTTA 
CCAGGCGACT CCCAAGTGAC CTTCACACTG GAGGTCAACC GGGAAAGTGA TCCCAGCACG 
GTGACCACGC AGACCGTGAC CATTACCACG GTCTACACCG ACCTCGACGC CCTAATCGAT 
GGCGGGAGTG AGCGGACCAT CGGCGTCGAC AGTGAGTCGT TCACACTGGA TGGATCGTCG 
TCGTTTGATC CCGACAGTGA AAATGAAGAG ATGACCTACC TCTGGACTTG TCAGAAGTCA 
AACACTGAGA CAGGCAGCTA CGAGCCTTGT GTATCAAGCG AGACACGTGA GGTGTTCCCG 
GCGGAAGCTG ACTCCAAAAC AAGTACTCTC ACGTTAGCTT CCAACAATCT TTACTCGGAC 
TCCACCTATC TGTTCACTCT GGAGATCAAC AAACTGTCAA GGACAGCATC GACCAGTGTC 
ACCATCCTTT CCAAGTCTGG CAATCCTCCA ACGATCAGTC TCGATGAATC CCTTCCTGAG 
AAGATAAAGA GCAGTAGTTC TCATACGCTA CGAGTATTGA TCAGCCATAC ATCAGATATT 
ACCATTGACT GGGCTACTGT CGACATAGAT CTTGGATATG GATACGTTGA CTTTTCACAG 
CTGAATAGTC AGTCATTCCT TATACAAGGA GATACATCAC CCTTCATCAC ATACGCCTTT 
GTAACTATCC CCACAGGAAC TTTGGAAGAG GATGTTGCCT ACTCTTTCTC AGTGACGGTA 
ACTGATACCG AAGGACAGGT GTCAGCTAGT AAATTGACTA CCAACACGTA TGGAGGAGTA 
TCATCCTGTA CATTCAGTTT GGGTGATGGT TCAACCAGCT ATCAAGAACT TGACGAGATC 
ACTCTCCTAA CTCAGAACTG CGTGACTGAT GAAGAAGCTT ACCCTCTGTC TTACCAAGTG 
TTCCGTGAGA AGGTCGAATC ATCTGTCACC CGTTACAACG CGATGACGGG TGCATTGGCA 
GAAGCGAGCA GCACTATATT GGGTCTGCCG TCCTTTAGCG ACAACCAGAA CACATTTGTT 
GTCAAGGTGT GCAATGATTT TGGAAGCTGT TCCTCGTACA GTCTTACATT GGACGTCACT 
CCCATCGCAT CTTTCAGTCA AGAACAGTTT GATGAAAGTA TAACCAATCT GGTAGAACCA 
CAAAAGCTCT CAGGGAATTT CCTAGATGCG CTGGTAAACT TCAACACCAT CACGTCAGTG 
GGTGTCGACA GCACGAGTAG GAAACGGAGA GCAGTCTCGG AGACATCCAG CACAGCTACT 
GAGCAGCTGG ATTTACTTCA GAATGCCATC TCGTCGACGG TCCTTGACAC GGCCACCGCC 
CAGACTCTGC TTGATCAGTC GGATGGCGTG GTCATTGCTG ATCTCACACT GTCTGATATG 
GACATCTACC TGGATATTTT AATTGAGCTC GTAGCTGTTT TCAGTAATGA AGGGCTTCCA 
GAGTCTAGCG CCGAGATCGT TCTTACAAAG ACAAGCGAGA TCGCTGCTGA ACTCGACCCT 
CAATACAACT CGGACATGCT TGCCAAAATT ACGACCCTGA GAAACACATT AATCCAGGGC 
CAGCTGAGTC AGATCCCACT TGGAGGGGAC CCTACGGAGA CCAGGTCTGG TTCCGTTACC 
ACCAGGGCCT TCTATGCTAT CCCTAGTGAT ACCCTCAGTA CTGCATCCAG CAACACCTCC 
TTCAACGTCA AGTTCGGAAG CGAGATCGAA AGCTTATATG GTGCAAGTTG GTCGTGTGCT 
TCTGGAAGCA CTTGTTCTGG TGTTCAGATC ATGTTTACTC AGTACGACGA CGAGGTTGAT 
TACTACTCGA CTACGGACGA GGATAAGGCG AACAGAGCAT CTTCTATCCT TGAAATCGAT 
CTGCTTGACC CGAGTGACAA TTCTGAGTTG AGTATTACTG GACTGAGCAG CCCCATATCA 
ATCAACATGA CCGCTTCACG CCTCCAATCC GACAAAGTCT ATGACTGCTA TTGGTGGGAC 
ACTAGCAGCA GCTCTTGGAG CCAGGATGGA CTTACGACCG TCTACCACTC ATCGGTCTTG 
TCTGAGTGCC AAACTACTCA TCTTTCAGCC TTCACCCTGA TCGCTGTTGA TTCTCCTACT 
ACGATTATGA TGGCTACCAC GATAGTTGAT GACGCCAGTA CTTCTACCGA TGCAGTAGCC 
TCGACTGCGA CAACGGAAAT CAGTGAGAGC GGCAGCAGTG GTCTAAGTTC TACCGTCATC 
ATCGTCATTG CCGTGGTCAT ATCTGTCGTT TTGATCCTAA TTATTATCAT CGCCATCGTG 
GTCGTGATGT CGAAGAAGTC CTCCAAGGTC GGTAATGCTG ATATCGAGAA CTCGAACGGC 
AATGGTGCCA GGAATGGGAC CAGCACCGCC GATGGACTCG CTCCTGTTGA ATCTGCGAAC 
CAGGCATTCA ATGCCCCACC AACAGCACCG GCCCACCCAA CCCATACTGT TCAAGTGGTC 
GCTGCCCCAG CTTCGGCACC TGCTTCTCAA GAGCCTATCA TGGACGTTGC AGACCTTCCT 
GGATCCCCAT CACCCCCTCC TGATGGACCC CATATTGATA CCATCATAAA GGATGTTCAG 
AATGATAGTA GGGCCCAAAC TCCAATGGAT AGAGCCATGA CGCCCGTTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REJ8 ( XM_011673694.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011671996.1 Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X2, mRNA.

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CloneID	OSd30108
Clone ID Related Accession (Same CDS sequence)	XM_011673694.1
Accession Version	XM_011673694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	receptor for egg jelly 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011984579.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC TTGTGGTGGT TGTCATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCATC 
ATCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATGG GACCCAAGCT TAGCTGGCTT ACCGGTGGAG CAATGCGAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAGT 
TCTTGGTCCT TCCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCAATCGAAA ATTTGGATTT TAACTGGACC AACTCTTTAT TCGATGATAC TGCGAATATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGAA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGATTGG TTCTAAACGT CATCGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG ATACAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGAAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATGTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAA TGAATTTCCC CGATTGGAGA 
GGCAATATGA GCGGGATTGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGACTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GAATGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AACTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGATTGATGA TTTATGTCAA 
TTGGTTCTTG AGGACAATTC CGATGCTGTT TACCAAGTCT GCATGGGTGA ACTTTTCCCA 
CTTATTGAGT CAATTCTAGA AGACGAGATG TATGATTTCA ATGATAATCA TACAAACACC 
GTTGTAGATT TATCGAACAT TGAGATCTGT CTCGATAATG GCGAGGTGAC TTTTGAGGCA 
CTTGAAAATT TAGATATATT GGCATATCTA CTACAAACCA CACACAAGCT CTATAATTTA 
ACCGTGTTCG ACGAGAGCAA CATTTGTAGA GCAGTGACCA GCGTAGTAGA ATCTAGAAAT 
GACATTCCGA CACTGATATC TGAATTTGCT GTGGAAATGA TGTCCCTCTT CATGCCGCTT 
GGTGCAGAAA TATGCTCTTG TTGGGATCAA GTTTTTGATG ATCTCTTCTC GGACTCCGAC 
GACTCGGAAT TTGGCGTAAA CCAATTCAAT GAGATAGTCC GGATTGCTGT GAACAGTATT 
GGATTAGACA GTCGTCATGA ACTCTGTGAC CATCTCATTC ATCTAAAGCC GGACAACATG 
TCTGATTACG CACAAACGGT GATTGAGGAA ATCCTTGGTT TTACGGCTGA TCCTGAGAAG 
TGTGCCTGTA CCGCCAATCG TGTCATCGAT TTCATCCTTC CCTTGGTTAA TGTTTCAATG 
GAGGAAATCA ATAACTACCT TTACCAGTAC CTGGGATACC ATTCTGTGGA TCACGTGTGT 
AGTTTCGTGG CCCAATCCTT CTCGGGAGTA ATAGTCGACC CTTCAAGCAT AAACTGTAAC 
AGCCAACAAG CCTGTCTCTT CATCACTGAC TGCGCCGGTG TATGCAACGG TAACGCTAGT 
TTGGATTGCG CCCGAGAATG CGGCGGCAGC GCGGCACTCG ACTGCGCCTT CAAGTGCGGT 
GGCTCTGCGA CCACGAACGA ATGTGGATGG TGCGTCGGTG GAGCTACGGA AAGACCAAGC 
ACTTTAGGTT TCGACCAGTG TGGTTCCTGC ATTGGGGAAG GCACTGCGGT CATCGACTGC 
AATGGTGACT GTGACGGTAC TGCTTACCGC GATGCCTGTC AAGTCTGCGT AGGTGGCAAT 
ACGAGTGTGG CTGAGGATGT AAGCGATCGT TTCTTGGACT GCCTAGGTAT CTGTAATGGT 
AGTTTTGTGA CCAATGATTG TGATGAATGC GTGCTGCCAT TCAGCGATGG GACTATTTTC 
TCCACGGCGG ATTGTAACGG TGACTGCAAT GGATTTGCCG ACATAAATGA GTGTAGTTAC 
TGTGTTGGGG GTAATACCGG TAACTCTGTG GATACTGGAT TGTCTGCATG TGGAGTCTGC 
AATACTACGA TCGAACCAGA CTTCTGCCTC GGCTGTGATG GCGTCGCTGA TAGTGGCCTA 
GTGACCGATG CGTGTGATGT ATGCGATGGG GATGGTAGCA CTTGTTTCAC CGTGGACAAT 
ATAGCTCCCT CTATCATCCC AGCTAACACC GACTATCAGG TTAAACTTCG TGGTGCCGGA 
TTCCAAGATG CGACTACCAA ACAGTGTAAG TTCCTGGATA GCAGCGGTGA GCAGGTCAGC 
GTGGTCGATA TCAGCGATGA GGTGGATGAC GAGTTCAACT GCACCGCTAA TCTTCCTCAA 
GGAACGTATT CTGTTTACCT TTCGACGGAG TCAACGGAAC TATCAGAAGC ATTCGTCACC 
CTCTATGTCT ATTCAGAAGT TATAATAAAC AGCTTATCCC AGAGCGAGTT TGATATAGAT 
GATAGTGTGA TTTCCATCTC TGTCAACATG GTATCAACAA GCGGAGCCTT TACGGCATAC 
AAGGACTTGA CCACTCCAAA AGCTATCGTC TTCACAGAGA CCGAGAGACT TTACTACGAA 
GGAACTTTTG GAGGAGACAA CGACGAGCTT CTCGAGTTTA CGGGTCCGAT GCTGACTACC 
TCGGCTCAAG TTGTCGTTTA CCCGTCCTTC AACGGAATCG ACTACCTGGT CACCCCCGAT 
GATGCTGGTT TCACGATCAC CTACTACGCC CCTGCACCGG TCGCTCAGAG TCTTAAATTC 
TCCCCTACTG GACATCTTCT GGAACTCGAG TTCGACGTCG CAATCAACGA AGACTCGGTG 
ACATCATGTG ATGCAGTATT CATGAATACC ACCAGCCTGG GTGGTGCTGC TGCCCAGTGC 
TTTGCATTCC CTTACCTAAA AACTATCTAT ATACTACCCT TCAGTTCGGC CAGTGTAAAT 
CTTATTGGAC CAGATGATGA GCTAGTGCTG CGCGCTGACG TTCTGAAAAA ACGTGATGAG 
GAATACGCTC GGTACGCCAG CGGTACTCTT ACCGCATCCT CCCCCGACGA GGCGGTAACC 
GTCTACGCGG CTCTGACCGG TACAGAAGCA ATTAGCAGCT GCGGTAATGT ACGACTTAGT 
GCTCTGCAAT CCTTTGGAAA CGCAGGACGT TTTTTCACCT ATCAGTGGTC TGTGACCAGT 
GGCGGTTCGA TCCCATCTGG CTTGACGGAT GATCTAACAG CCGCAGCGGA GTCCGCCTAT 
GTCACTCTCG ATGGATCCTA CCTCGACGCG GACGTGCCGT ATACATTCAG CATGACAGCA 
GAGAACTTCC TCGGAGGCAG TGACACAAAG ACCTTGGAGG TGACTCGGTC TGCTCTCAAC 
AAACCAGACG TCGTGATCTA CCCGGAAAGA GTTGATCCTA ATAGTGCCCT TATCTCGCAA 
GCGTTCTACC TCCGCGCTGC TGTACGATTC TACGAGGCCT GCGGTGGAGC TGAGCAGATG 
GAGTATGCCT GGTCTGTAGA TGATGATAGT ATTGCTCTTA ACCTACAAAC CATAAACAAA 
CCACTCCTGT ATGTGGCAGC TGGTTCTTTA CCAGGCGACT CCCAAGTGAC CTTCACACTG 
GAGGTCAACC GGGAAAGTGA TCCCAGCACG GTGACCACGC AGACCGTGAC CATTACCACG 
GTCTACACCG ACCTCGACGC CCTAATCGAT GGCGGGAGTG AGCGGACCAT CGGCGTCGAC 
AGTGAGTCGT TCACACTGGA TGGATCGTCG TCGTTTGATC CCGACAGTGA AAATGAAGAG 
ATGACCTACC TCTGGACTTG TCAGAAGTCA AACACTGAGA CAGGCAGCTA CGAGCCTTGT 
GTATCAAGCG AGACACGTGA GGTGTTCCCG GCGGAAGCTG ACTCCAAAAC AAGTACTCTC 
ACGTTAGCTT CCAACAATCT TTACTCGGAC TCCACCTATC TGTTCACTCT GGAGATCAAC 
AAACTGTCAA GGACAGCATC GACCAGTGTC ACCATCCTTT CCAAGTCTGG CAATCCTCCA 
ACGATCAGTC TCGATGAATC CCTTCCTGAG AAGATAAAGA GCAGTAGTTC TCATACGCTA 
CGAGTATTGA TCAGCCATAC ATCAGATATT ACCATTGACT GGGCTACTGT CGACATAGAT 
CTTGGATATG GATACGTTGA CTTTTCACAG CTGAATAGTC AGTCATTCCT TATACAAGGA 
GATACATCAC CCTTCATCAC ATACGCCTTT GTAACTATCC CCACAGGAAC TTTGGAAGAG 
GATGTTGCCT ACTCTTTCTC AGTGACGGTA ACTGATACCG AAGGACAGGT GTCAGCTAGT 
AAATTGACTA CCAACACGTA TGGAGGAGTA TCATCCTGTA CATTCAGTTT GGGTGATGGT 
TCAACCAGCT ATCAAGAACT TGACGAGATC ACTCTCCTAA CTCAGAACTG CGTGACTGAT 
GAAGAAGCTT ACCCTCTGTC TTACCAAGTG TTCCGTGAGA AGGTCGAATC ATCTGTCACC 
CGTTACAACG CGATGACGGG TGCATTGGCA GAAGCGAGCA GCACTATATT GGGTCTGCCG 
TCCTTTAGCG ACAACCAGAA CACATTTGTT GTCAAGGTGT GCAATGATTT TGGAAGCTGT 
TCCTCGTACA GTCTTACATT GGACGTCACT CCCATCGCAT CTTTCAGTCA AGAACAGTTT 
GATGAAAGTA TAACCAATCT GGTAGAACCA CAAAAGCTCT CAGGGAATTT CCTAGATGCG 
CTGGTAAACT TCAACACCAT CACGTCAGTG GGTGTCGACA GCACGAGTAG GAAACGGAGA 
GCAGTCTCGG AGACATCCAG CACAGCTACT GAGCAGCTGG ATTTACTTCA GAATGCCATC 
TCGTCGACGG TCCTTGACAC GGCCACCGCC CAGACTCTGC TTGATCAGTC GGATGGCGTG 
GTCATTGCTG ATCTCACACT GTCTGATATG GACATCTACC TGGATATTTT AATTGAGCTC 
GTAGCTGTTT TCAGTAATGA AGGGCTTCCA GAGTCTAGCG CCGAGATCGT TCTTACAAAG 
ACAAGCGAGA TCGCTGCTGA ACTCGACCCT CAATACAACT CGGACATGCT TGCCAAAATT 
ACGACCCTGA GAAACACATT AATCCAGGGC CAGCTGAGTC AGATCCCACT TGGAGGGGAC 
CCTACGGAGA CCAGGTCTGG TTCCGTTACC ACCAGGGCCT TCTATGCTAT CCCTAGTGAT 
ACCCTCAGTA CTGCATCCAG CAACACCTCC TTCAACGTCA AGTTCGGAAG CGAGATCGAA 
AGCTTATATG GTGCAAGTTG GTCGTGTGCT TCTGGAAGCA CTTGTTCTGG TGTTCAGATC 
ATGTTTACTC AGTACGACGA CGAGGTTGAT TACTACTCGA CTACGGACGA GGATAAGGCG 
AACAGAGCAT CTTCTATCCT TGAAATCGAT CTGCTTGACC CGAGTGACAA TTCTGAGTTG 
AGTATTACTG GACTGAGCAG CCCCATATCA ATCAACATGA CCGCTTCACG CCTCCAATCC 
GACAAAGTCT ATGACTGCTA TTGGTGGGAC ACTAGCAGCA GCTCTTGGAG CCAGGATGGA 
CTTACGACCG TCTACCACTC ATCGGTCTTG TCTGAGTGCC AAACTACTCA TCTTTCAGCC 
TTCACCCTGA TCGCTGTTGA TTCTCCTACT ACGATTATGA TGGCTACCAC GATAGTTGAT 
GACGCCAGTA CTTCTACCGA TGCAGTAGCC TCGACTGCGA CAACGGAAAT CAGTGAGAGC 
GGCAGCAGTG GTCTAAGTTC TACCGTCATC ATCGTCATTG CCGTGGTCAT ATCTGTCGTT 
TTGATCCTAA TTATTATCAT CGCCATCGTG GTCGTGATGT CGAAGAAGTC CTCCAAGGTC 
GGTAATGCTG ATATCGAGAA CTCGAACGGC AATGGTGCCA GGAATGGGAC CAGCACCGCC 
GATGGACTCG CTCCTGTTGA ATCTGCGAAC CAGGCATTCA ATGCCCCACC AACAGCACCG 
GCCCACCCAA CCCATACTGT TCAAGTGGTC GCTGCCCCAG CTTCGGCACC TGCTTCTCAA 
GAGCCTATCA TGGACGTTGC AGACCTTCCT GGATCCCCAT CACCCCCTCC TGATGGACCC 
CATATTGATA CCATCATAAA GGATGTTCAG AATGATAGTA GGGCCCAAAC TCCAATGGAT 
AGAGCCATGA CGCCCGTTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011671996.1
CDS	198..8921
Translation	MKALVVVVIFSCLVAGAARAEESTEWQVEWTTEPAYNLSIIEPFTVPGYLLPDLNISDIISTVGMMYQAEDLVHAIGSLCVEMGPKLSWLTGGAMRELCDPVLEALRNGSYAEAYSICQSSWSFLETLIGGEEGGLEGEGEIDHGPIRRKRWGLSVPSIELPPLPSSPIQAVLNITVPFSPIENLDFNWTNSLFDDTANMVENIGLIPDLNRNWSFPIFDGTGIEVDFPYPEFSDKYGICSSLAGLISYNQPSSPSHGSIFGLVLNVIVAEQVRSIPGKCLSFFDGEEGSGDIDDDPSFKHEPDTMIWAFATLGGFDDIEQICNVSSWALLMRDEGKFELFNELVNQITTSIYSVISSRSRCIEFADSLHANIGLAPGNYSHVTSSEALCDEIMNFPDWRGNMSGIDFYNYDSMDEKDAFIYLLSIVEILSHKSDMASLEFLCRMSPYADEMFCTSLAYNDYRDVSREDIIEHCLRYVAFDGFEFDFLALLNAIGAEFDFDFYDFDQTCSHLTDIVFNSVIRQQFTIDNMIQKGMKAIGRVLSPIFCDLHSFRNGSLDSLEAPCGTPGRGCFNWTRPGEDYFFDRLDEGFIVGTIFSYLGGYRDGDHICHQMAFALDDETVLDMVAGKLRDSVYRLFDDVPFCSDTIGLIEKLIGVDEHAAQMIGFNSTDAFCGEISKIYHQGGNYNFSFVPLLDLFTNHSHGLEHFVTSESIMHVTNEMMKFAAMIMHSDNIGDAISKSCYMWQDSGPGITMPLFEVDDLCQVVLALNPAELQDVCIHDIIPWIEDLFDHHHDDEERGEENEIDFDFTGILETVGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFYDLPASHLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALEDDAQLDMVANKMKDSIFRFFNDPSFCPVTMDLINRLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSLEPLYVLFANVEEEGEFDPAAIINATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEIDDLCQLVLEDNSDAVYQVCMGELFPLIESILEDEMYDFNDNHTNTVVDLSNIEICLDNGEVTFEALENLDILAYLLQTTHKLYNLTVFDESNICRAVTSVVESRNDIPTLISEFAVEMMSLFMPLGAEICSCWDQVFDDLFSDSDDSEFGVNQFNEIVRIAVNSIGLDSRHELCDHLIHLKPDNMSDYAQTVIEEILGFTADPEKCACTANRVIDFILPLVNVSMEEINNYLYQYLGYHSVDHVCSFVAQSFSGVIVDPSSINCNSQQACLFITDCAGVCNGNASLDCARECGGSAALDCAFKCGGSATTNECGWCVGGATERPSTLGFDQCGSCIGEGTAVIDCNGDCDGTAYRDACQVCVGGNTSVAEDVSDRFLDCLGICNGSFVTNDCDECVLPFSDGTIFSTADCNGDCNGFADINECSYCVGGNTGNSVDTGLSACGVCNTTIEPDFCLGCDGVADSGLVTDACDVCDGDGSTCFTVDNIAPSIIPANTDYQVKLRGAGFQDATTKQCKFLDSSGEQVSVVDISDEVDDEFNCTANLPQGTYSVYLSTESTELSEAFVTLYVYSEVIINSLSQSEFDIDDSVISISVNMVSTSGAFTAYKDLTTPKAIVFTETERLYYEGTFGGDNDELLEFTGPMLTTSAQVVVYPSFNGIDYLVTPDDAGFTITYYAPAPVAQSLKFSPTGHLLELEFDVAINEDSVTSCDAVFMNTTSLGGAAAQCFAFPYLKTIYILPFSSASVNLIGPDDELVLRADVLKKRDEEYARYASGTLTASSPDEAVTVYAALTGTEAISSCGNVRLSALQSFGNAGRFFTYQWSVTSGGSIPSGLTDDLTAAAESAYVTLDGSYLDADVPYTFSMTAENFLGGSDTKTLEVTRSALNKPDVVIYPERVDPNSALISQAFYLRAAVRFYEACGGAEQMEYAWSVDDDSIALNLQTINKPLLYVAAGSLPGDSQVTFTLEVNRESDPSTVTTQTVTITTVYTDLDALIDGGSERTIGVDSESFTLDGSSSFDPDSENEEMTYLWTCQKSNTETGSYEPCVSSETREVFPAEADSKTSTLTLASNNLYSDSTYLFTLEINKLSRTASTSVTILSKSGNPPTISLDESLPEKIKSSSSHTLRVLISHTSDITIDWATVDIDLGYGYVDFSQLNSQSFLIQGDTSPFITYAFVTIPTGTLEEDVAYSFSVTVTDTEGQVSASKLTTNTYGGVSSCTFSLGDGSTSYQELDEITLLTQNCVTDEEAYPLSYQVFREKVESSVTRYNAMTGALAEASSTILGLPSFSDNQNTFVVKVCNDFGSCSSYSLTLDVTPIASFSQEQFDESITNLVEPQKLSGNFLDALVNFNTITSVGVDSTSRKRRAVSETSSTATEQLDLLQNAISSTVLDTATAQTLLDQSDGVVIADLTLSDMDIYLDILIELVAVFSNEGLPESSAEIVLTKTSEIAAELDPQYNSDMLAKITTLRNTLIQGQLSQIPLGGDPTETRSGSVTTRAFYAIPSDTLSTASSNTSFNVKFGSEIESLYGASWSCASGSTCSGVQIMFTQYDDEVDYYSTTDEDKANRASSILEIDLLDPSDNSELSITGLSSPISINMTASRLQSDKVYDCYWWDTSSSSWSQDGLTTVYHSSVLSECQTTHLSAFTLIAVDSPTTIMMATTIVDDASTSTDAVASTATTEISESGSSGLSSTVIIVIAVVISVVLILIIIIAIVVVMSKKSSKVGNADIENSNGNGARNGTSTADGLAPVESANQAFNAPPTAPAHPTHTVQVVAAPASAPASQEPIMDVADLPGSPSPPPDGPHIDTIIKDVQNDSRAQTPMDRAMTPVE

Target ORF information:

RefSeq Version	XM_011673694.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011673694.1

ORF Insert Sequence:

ATGAAGGCAC TTGTGGTGGT TGTCATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCATC 
ATCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATGG GACCCAAGCT TAGCTGGCTT ACCGGTGGAG CAATGCGAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAGT 
TCTTGGTCCT TCCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCAATCGAAA ATTTGGATTT TAACTGGACC AACTCTTTAT TCGATGATAC TGCGAATATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGAA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGATTGG TTCTAAACGT CATCGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG ATACAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGAAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATGTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAA TGAATTTCCC CGATTGGAGA 
GGCAATATGA GCGGGATTGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGACTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GAATGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AACTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGATTGATGA TTTATGTCAA 
TTGGTTCTTG AGGACAATTC CGATGCTGTT TACCAAGTCT GCATGGGTGA ACTTTTCCCA 
CTTATTGAGT CAATTCTAGA AGACGAGATG TATGATTTCA ATGATAATCA TACAAACACC 
GTTGTAGATT TATCGAACAT TGAGATCTGT CTCGATAATG GCGAGGTGAC TTTTGAGGCA 
CTTGAAAATT TAGATATATT GGCATATCTA CTACAAACCA CACACAAGCT CTATAATTTA 
ACCGTGTTCG ACGAGAGCAA CATTTGTAGA GCAGTGACCA GCGTAGTAGA ATCTAGAAAT 
GACATTCCGA CACTGATATC TGAATTTGCT GTGGAAATGA TGTCCCTCTT CATGCCGCTT 
GGTGCAGAAA TATGCTCTTG TTGGGATCAA GTTTTTGATG ATCTCTTCTC GGACTCCGAC 
GACTCGGAAT TTGGCGTAAA CCAATTCAAT GAGATAGTCC GGATTGCTGT GAACAGTATT 
GGATTAGACA GTCGTCATGA ACTCTGTGAC CATCTCATTC ATCTAAAGCC GGACAACATG 
TCTGATTACG CACAAACGGT GATTGAGGAA ATCCTTGGTT TTACGGCTGA TCCTGAGAAG 
TGTGCCTGTA CCGCCAATCG TGTCATCGAT TTCATCCTTC CCTTGGTTAA TGTTTCAATG 
GAGGAAATCA ATAACTACCT TTACCAGTAC CTGGGATACC ATTCTGTGGA TCACGTGTGT 
AGTTTCGTGG CCCAATCCTT CTCGGGAGTA ATAGTCGACC CTTCAAGCAT AAACTGTAAC 
AGCCAACAAG CCTGTCTCTT CATCACTGAC TGCGCCGGTG TATGCAACGG TAACGCTAGT 
TTGGATTGCG CCCGAGAATG CGGCGGCAGC GCGGCACTCG ACTGCGCCTT CAAGTGCGGT 
GGCTCTGCGA CCACGAACGA ATGTGGATGG TGCGTCGGTG GAGCTACGGA AAGACCAAGC 
ACTTTAGGTT TCGACCAGTG TGGTTCCTGC ATTGGGGAAG GCACTGCGGT CATCGACTGC 
AATGGTGACT GTGACGGTAC TGCTTACCGC GATGCCTGTC AAGTCTGCGT AGGTGGCAAT 
ACGAGTGTGG CTGAGGATGT AAGCGATCGT TTCTTGGACT GCCTAGGTAT CTGTAATGGT 
AGTTTTGTGA CCAATGATTG TGATGAATGC GTGCTGCCAT TCAGCGATGG GACTATTTTC 
TCCACGGCGG ATTGTAACGG TGACTGCAAT GGATTTGCCG ACATAAATGA GTGTAGTTAC 
TGTGTTGGGG GTAATACCGG TAACTCTGTG GATACTGGAT TGTCTGCATG TGGAGTCTGC 
AATACTACGA TCGAACCAGA CTTCTGCCTC GGCTGTGATG GCGTCGCTGA TAGTGGCCTA 
GTGACCGATG CGTGTGATGT ATGCGATGGG GATGGTAGCA CTTGTTTCAC CGTGGACAAT 
ATAGCTCCCT CTATCATCCC AGCTAACACC GACTATCAGG TTAAACTTCG TGGTGCCGGA 
TTCCAAGATG CGACTACCAA ACAGTGTAAG TTCCTGGATA GCAGCGGTGA GCAGGTCAGC 
GTGGTCGATA TCAGCGATGA GGTGGATGAC GAGTTCAACT GCACCGCTAA TCTTCCTCAA 
GGAACGTATT CTGTTTACCT TTCGACGGAG TCAACGGAAC TATCAGAAGC ATTCGTCACC 
CTCTATGTCT ATTCAGAAGT TATAATAAAC AGCTTATCCC AGAGCGAGTT TGATATAGAT 
GATAGTGTGA TTTCCATCTC TGTCAACATG GTATCAACAA GCGGAGCCTT TACGGCATAC 
AAGGACTTGA CCACTCCAAA AGCTATCGTC TTCACAGAGA CCGAGAGACT TTACTACGAA 
GGAACTTTTG GAGGAGACAA CGACGAGCTT CTCGAGTTTA CGGGTCCGAT GCTGACTACC 
TCGGCTCAAG TTGTCGTTTA CCCGTCCTTC AACGGAATCG ACTACCTGGT CACCCCCGAT 
GATGCTGGTT TCACGATCAC CTACTACGCC CCTGCACCGG TCGCTCAGAG TCTTAAATTC 
TCCCCTACTG GACATCTTCT GGAACTCGAG TTCGACGTCG CAATCAACGA AGACTCGGTG 
ACATCATGTG ATGCAGTATT CATGAATACC ACCAGCCTGG GTGGTGCTGC TGCCCAGTGC 
TTTGCATTCC CTTACCTAAA AACTATCTAT ATACTACCCT TCAGTTCGGC CAGTGTAAAT 
CTTATTGGAC CAGATGATGA GCTAGTGCTG CGCGCTGACG TTCTGAAAAA ACGTGATGAG 
GAATACGCTC GGTACGCCAG CGGTACTCTT ACCGCATCCT CCCCCGACGA GGCGGTAACC 
GTCTACGCGG CTCTGACCGG TACAGAAGCA ATTAGCAGCT GCGGTAATGT ACGACTTAGT 
GCTCTGCAAT CCTTTGGAAA CGCAGGACGT TTTTTCACCT ATCAGTGGTC TGTGACCAGT 
GGCGGTTCGA TCCCATCTGG CTTGACGGAT GATCTAACAG CCGCAGCGGA GTCCGCCTAT 
GTCACTCTCG ATGGATCCTA CCTCGACGCG GACGTGCCGT ATACATTCAG CATGACAGCA 
GAGAACTTCC TCGGAGGCAG TGACACAAAG ACCTTGGAGG TGACTCGGTC TGCTCTCAAC 
AAACCAGACG TCGTGATCTA CCCGGAAAGA GTTGATCCTA ATAGTGCCCT TATCTCGCAA 
GCGTTCTACC TCCGCGCTGC TGTACGATTC TACGAGGCCT GCGGTGGAGC TGAGCAGATG 
GAGTATGCCT GGTCTGTAGA TGATGATAGT ATTGCTCTTA ACCTACAAAC CATAAACAAA 
CCACTCCTGT ATGTGGCAGC TGGTTCTTTA CCAGGCGACT CCCAAGTGAC CTTCACACTG 
GAGGTCAACC GGGAAAGTGA TCCCAGCACG GTGACCACGC AGACCGTGAC CATTACCACG 
GTCTACACCG ACCTCGACGC CCTAATCGAT GGCGGGAGTG AGCGGACCAT CGGCGTCGAC 
AGTGAGTCGT TCACACTGGA TGGATCGTCG TCGTTTGATC CCGACAGTGA AAATGAAGAG 
ATGACCTACC TCTGGACTTG TCAGAAGTCA AACACTGAGA CAGGCAGCTA CGAGCCTTGT 
GTATCAAGCG AGACACGTGA GGTGTTCCCG GCGGAAGCTG ACTCCAAAAC AAGTACTCTC 
ACGTTAGCTT CCAACAATCT TTACTCGGAC TCCACCTATC TGTTCACTCT GGAGATCAAC 
AAACTGTCAA GGACAGCATC GACCAGTGTC ACCATCCTTT CCAAGTCTGG CAATCCTCCA 
ACGATCAGTC TCGATGAATC CCTTCCTGAG AAGATAAAGA GCAGTAGTTC TCATACGCTA 
CGAGTATTGA TCAGCCATAC ATCAGATATT ACCATTGACT GGGCTACTGT CGACATAGAT 
CTTGGATATG GATACGTTGA CTTTTCACAG CTGAATAGTC AGTCATTCCT TATACAAGGA 
GATACATCAC CCTTCATCAC ATACGCCTTT GTAACTATCC CCACAGGAAC TTTGGAAGAG 
GATGTTGCCT ACTCTTTCTC AGTGACGGTA ACTGATACCG AAGGACAGGT GTCAGCTAGT 
AAATTGACTA CCAACACGTA TGGAGGAGTA TCATCCTGTA CATTCAGTTT GGGTGATGGT 
TCAACCAGCT ATCAAGAACT TGACGAGATC ACTCTCCTAA CTCAGAACTG CGTGACTGAT 
GAAGAAGCTT ACCCTCTGTC TTACCAAGTG TTCCGTGAGA AGGTCGAATC ATCTGTCACC 
CGTTACAACG CGATGACGGG TGCATTGGCA GAAGCGAGCA GCACTATATT GGGTCTGCCG 
TCCTTTAGCG ACAACCAGAA CACATTTGTT GTCAAGGTGT GCAATGATTT TGGAAGCTGT 
TCCTCGTACA GTCTTACATT GGACGTCACT CCCATCGCAT CTTTCAGTCA AGAACAGTTT 
GATGAAAGTA TAACCAATCT GGTAGAACCA CAAAAGCTCT CAGGGAATTT CCTAGATGCG 
CTGGTAAACT TCAACACCAT CACGTCAGTG GGTGTCGACA GCACGAGTAG GAAACGGAGA 
GCAGTCTCGG AGACATCCAG CACAGCTACT GAGCAGCTGG ATTTACTTCA GAATGCCATC 
TCGTCGACGG TCCTTGACAC GGCCACCGCC CAGACTCTGC TTGATCAGTC GGATGGCGTG 
GTCATTGCTG ATCTCACACT GTCTGATATG GACATCTACC TGGATATTTT AATTGAGCTC 
GTAGCTGTTT TCAGTAATGA AGGGCTTCCA GAGTCTAGCG CCGAGATCGT TCTTACAAAG 
ACAAGCGAGA TCGCTGCTGA ACTCGACCCT CAATACAACT CGGACATGCT TGCCAAAATT 
ACGACCCTGA GAAACACATT AATCCAGGGC CAGCTGAGTC AGATCCCACT TGGAGGGGAC 
CCTACGGAGA CCAGGTCTGG TTCCGTTACC ACCAGGGCCT TCTATGCTAT CCCTAGTGAT 
ACCCTCAGTA CTGCATCCAG CAACACCTCC TTCAACGTCA AGTTCGGAAG CGAGATCGAA 
AGCTTATATG GTGCAAGTTG GTCGTGTGCT TCTGGAAGCA CTTGTTCTGG TGTTCAGATC 
ATGTTTACTC AGTACGACGA CGAGGTTGAT TACTACTCGA CTACGGACGA GGATAAGGCG 
AACAGAGCAT CTTCTATCCT TGAAATCGAT CTGCTTGACC CGAGTGACAA TTCTGAGTTG 
AGTATTACTG GACTGAGCAG CCCCATATCA ATCAACATGA CCGCTTCACG CCTCCAATCC 
GACAAAGTCT ATGACTGCTA TTGGTGGGAC ACTAGCAGCA GCTCTTGGAG CCAGGATGGA 
CTTACGACCG TCTACCACTC ATCGGTCTTG TCTGAGTGCC AAACTACTCA TCTTTCAGCC 
TTCACCCTGA TCGCTGTTGA TTCTCCTACT ACGATTATGA TGGCTACCAC GATAGTTGAT 
GACGCCAGTA CTTCTACCGA TGCAGTAGCC TCGACTGCGA CAACGGAAAT CAGTGAGAGC 
GGCAGCAGTG GTCTAAGTTC TACCGTCATC ATCGTCATTG CCGTGGTCAT ATCTGTCGTT 
TTGATCCTAA TTATTATCAT CGCCATCGTG GTCGTGATGT CGAAGAAGTC CTCCAAGGTC 
GGTAATGCTG ATATCGAGAA CTCGAACGGC AATGGTGCCA GGAATGGGAC CAGCACCGCC 
GATGGACTCG CTCCTGTTGA ATCTGCGAAC CAGGCATTCA ATGCCCCACC AACAGCACCG 
GCCCACCCAA CCCATACTGT TCAAGTGGTC GCTGCCCCAG CTTCGGCACC TGCTTCTCAA 
GAGCCTATCA TGGACGTTGC AGACCTTCCT GGATCCCCAT CACCCCCTCC TGATGGACCC 
CATATTGATA CCATCATAAA GGATGTTCAG AATGATAGTA GGGCCCAAAC TCCAATGGAT 
AGAGCCATGA CGCCCGTTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REJ8 ( XM_011673695.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011671997.1 Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X3, mRNA.

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CloneID	OSd30109
Clone ID Related Accession (Same CDS sequence)	XM_011673695.1
Accession Version	XM_011673695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	receptor for egg jelly 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011984579.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC TTGTGGTGGT TGTCATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCATC 
ATCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATGG GACCCAAGCT TAGCTGGCTT ACCGGTGGAG CAATGCGAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAGT 
TCTTGGTCCT TCCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCAATCGAAA ATTTGGATTT TAACTGGACC AACTCTTTAT TCGATGATAC TGCGAATATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGAA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGATTGG TTCTAAACGT CATCGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG ATACAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGAAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATGTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAA TGAATTTCCC CGATTGGAGA 
GGCAATATGA GCGGGATTGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGACTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GAATGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AACTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGGTTGACGA TTTATGTAAA 
ACAGTATTGG CCTTTAATTC TGCAGAACTC CAAGATGTCT GCATCCACGA ACTATTTCCC 
TGGATTGAGG ACGTTTTTGA CCACCATGAT GATGATGAAA TGAGGGAAGA AGAAAACGAA 
ATTGACTTTG ATTTCACAGG GATTCTCGAA ACGGTAGGCG AGGAATTCGG ATTCGACTTT 
CACGATTCTC ACCAAAGTTG CTCTCATCTT ACTGGTATTC TCTTTGATGA TACCGTCAGC 
CAAAACTTTC CAATCGACAG CGTTGTGGTA AGGGGAATAA GAGCTGTTGG TAAGATAGTA 
TCACCCATAT TCTGTGATTT TGAATCATTT CATGACCACC CAGATACTGT CTTGGAAGCT 
CCGTGTGCAA CTCCTGGACA TAACTGCTTT GATTGGTCAA GCCGAGGAGA AGACTTCTTC 
TATGAGCGGG AAGACATTGG CTTTGTTATT GGTACAATTT TCAGTTATAT TGGAGGCTAC 
AGAGATGGGG ACCACCTTTG TCACCAAATG GTATTTGCAC TGGAGGATGA TGCACAACTT 
GACATGGTCG CAAATAAAAT GAAGGGTAGT ATCTTTCGAT TCTTCAACGA CCCGTCATTC 
TGTCCTGTGA CGATGGACAT TATCAACAGG CTCATCGGAT TTGACGAGGA TGCTGCAAGT 
TTACTCGGCT TTGAGAATGC GGATGCCTTC TGCGGAAAGG TCAATCAAAT ATACAATGAA 
GATGGCGATT ATAACGTCAG TTTTGAGCCT CTCTATGTTT TGTTTGCCAA TGTTGAAGAA 
GAAGGAGAAT TTGACCCTGC AGCCATTATA AATGCCACAA CCGAGATGAT TAAATTCGCC 
GCCATGATCA TGCGTTCAGA CCACATTGGA GATGCCATTT CTAAAACATG CTACGTGTGG 
CAGGAATCTG CTGCAATGTT AAATATTTCA GAGATTGATG ATTTATGTCA ATTGGTTCTT 
GAGGACAATT CCGATGCTGT TTACCAAGTC TGCATGGGTG AACTTTTCCC ACTTATTGAG 
TCAATTCTAG AAGACGAGAT GTATGATTTC AATGATAATC ATACAAACAC CGTTGTAGAT 
TTATCGAACA TTGAGATCTG TCTCGATAAT GGCGAGGTGA CTTTTGAGGC ACTTGAAAAT 
TTAGATATAT TGGCATATCT ACTACAAACC ACACACAAGC TCTATAATTT AACCGTGTTC 
GACGAGAGCA ACATTTGTAG AGCAGTGACC AGCGTAGTAG AATCTAGAAA TGACATTCCG 
ACACTGATAT CTGAATTTGC TGTGGAAATG ATGTCCCTCT TCATGCCGCT TGGTGCAGAA 
ATATGCTCTT GTTGGGATCA AGTTTTTGAT GATCTCTTCT CGGACTCCGA CGACTCGGAA 
TTTGGCGTAA ACCAATTCAA TGAGATAGTC CGGATTGCTG TGAACAGTAT TGGATTAGAC 
AGTCGTCATG AACTCTGTGA CCATCTCATT CATCTAAAGC CGGACAACAT GTCTGATTAC 
GCACAAACGG TGATTGAGGA AATCCTTGGT TTTACGGCTG ATCCTGAGAA GTGTGCCTGT 
ACCGCCAATC GTGTCATCGA TTTCATCCTT CCCTTGGTTA ATGTTTCAAT GGAGGAAATC 
AATAACTACC TTTACCAGTA CCTGGGATAC CATTCTGTGG ATCACGTGTG TAGTTTCGTG 
GCCCAATCCT TCTCGGGAGT AATAGTCGAC CCTTCAAGCA TAAACTGTAA CAGCCAACAA 
GAACCAACAA CCAAAGCTAC GACCACAACT CAATCATCAT TAGACAATAA TGGAAACTCA 
ACTCAATCAT CAATAGACAA TACTGGAAAC TCAACTCAAT CACCAATAGA CAATACTGGA 
GACTCGAATG AAACTTCGAC TGACGACGAA ACCTGTCTCT TCATCACTGA CTGCGCCGGT 
GTATGCAACG GTAACGCTAG TTTGGATTGC GCCCGAGAAT GCGGCGGCAG CGCGGCACTC 
GACTGCGCCT TCAAGTGCGG TGGCTCTGCG ACCACGAACG AATGTGGATG GTGCGTCGGT 
GGAGCTACGG AAAGACCAAG CACTTTAGGT TTCGACCAGT GTGGTTCCTG CATTGGGGAA 
GGCACTGCGG TCATCGACTG CAATGGTGAC TGTGACGGTA CTGCTTACCG CGATGCCTGT 
CAAGTCTGCG TAGGTGGCAA TACGAGTGTG GCTGAGGATG TAAGCGATCG TTTCTTGGAC 
TGCCTAGGTA TCTGTAATGG TAGTTTTGTG ACCAATGATT GTGATGAATG CGTGCTGCCA 
TTCAGCGATG GGACTATTTT CTCCACGGCG GATTGTAACG GTGACTGCAA TGGATTTGCC 
GACATAAATG AGTGTAGTTA CTGTGTTGGG GGTAATACCG GTAACTCTGT GGATACTGGA 
TTGTCTGCAT GTGGAGTCTG CAATACTACG ATCGAACCAG ACTTCTGCCT CGGCTGTGAT 
GGCGTCGCTG ATAGTGGCCT AGTGACCGAT GCGTGTGATG TATGCGATGG GGATGGTAGC 
ACTTGTTTCA CCGTGGACAA TATAGCTCCC TCTATCATCC CAGCTAACAC CGACTATCAG 
GTTAAACTTC GTGGTGCCGG ATTCCAAGAT GCGACTACCA AACAGTGTAA GTTCCTGGAT 
AGCAGCGGTG AGCAGGTCAG CGTGGTCGAT ATCAGCGATG AGGTGGATGA CGAGTTCAAC 
TGCACCGCTA ATCTTCCTCA AGGAACGTAT TCTGTTTACC TTTCGACGGA GTCAACGGAA 
CTATCAGAAG CATTCGTCAC CCTCTATGTC TATTCAGAAG TTATAATAAA CAGCTTATCC 
CAGAGCGAGT TTGATATAGA TGATAGTGTG ATTTCCATCT CTGTCAACAT GGTATCAACA 
AGCGGAGCCT TTACGGCATA CAAGGACTTG ACCACTCCAA AAGCTATCGT CTTCACAGAG 
ACCGAGAGAC TTTACTACGA AGGAACTTTT GGAGGAGACA ACGACGAGCT TCTCGAGTTT 
ACGGGTCCGA TGCTGACTAC CTCGGCTCAA GTTGTCGTTT ACCCGTCCTT CAACGGAATC 
GACTACCTGG TCACCCCCGA TGATGCTGGT TTCACGATCA CCTACTACGC CCCTGCACCG 
GTCGCTCAGA GTCTTAAATT CTCCCCTACT GGACATCTTC TGGAACTCGA GTTCGACGTC 
GCAATCAACG AAGACTCGGT GACATCATGT GATGCAGTAT TCATGAATAC CACCAGCCTG 
GGTGGTGCTG CTGCCCAGTG CTTTGCATTC CCTTACCTAA AAACTATCTA TATACTACCC 
TTCAGTTCGG CCAGTGTAAA TCTTATTGGA CCAGATGATG AGCTAGTGCT GCGCGCTGAC 
GTTCTGAAAA AACGTGATGA GGAATACGCT CGGTACGCCA GCGGTACTCT TACCGCATCC 
TCCCCCGACG AGGCGGTAAC CGTCTACGCG GCTCTGACCG GTACAGAAGC AATTAGCAGC 
TGCGGTAATG TACGACTTAG TGCTCTGCAA TCCTTTGGAA ACGCAGGACG TTTTTTCACC 
TATCAGTGGT CTGTGACCAG TGGCGGTTCG ATCCCATCTG GCTTGACGGA TGATCTAACA 
GCCGCAGCGG AGTCCGCCTA TGTCACTCTC GATGGATCCT ACCTCGACGC GGACGTGCCG 
TATACATTCA GCATGACAGC AGAGAACTTC CTCGGAGGCA GTGACACAAA GACCTTGGAG 
GTGACTCGGT CTGCTCTCAA CAAACCAGAC GTCGTGATCT ACCCGGAAAG AGTTGATCCT 
AATAGTGCCC TTATCTCGCA AGCGTTCTAC CTCCGCGCTG CTGTACGATT CTACGAGGCC 
TGCGGTGGAG CTGAGCAGAT GGAGTATGCC TGGTCTGTAG ATGATGATAG TATTGCTCTT 
AACCTACAAA CCATAAACAA ACCACTCCTG TATGTGGCAG CTGGTTCTTT ACCAGGCGAC 
TCCCAAGTGA CCTTCACACT GGAGGTCAAC CGGGAAAGTG ATCCCAGCAC GGTGACCACG 
CAGACCGTGA CCATTACCAC GGTCTACACC GACCTCGACG CCCTAATCGA TGGCGGGAGT 
GAGCGGACCA TCGGCGTCGA CAGTGAGTCG TTCACACTGG ATGGATCGTC GTCGTTTGAT 
CCCGACAGTG AAAATGAAGA GATGACCTAC CTCTGGACTT GTCAGAAGTC AAACACTGAG 
ACAGGCAGCT ACGAGCCTTG TGTATCAAGC GAGACACGTG AGGTGTTCCC GGCGGAAGCT 
GACTCCAAAA CAAGTACTCT CACGTTAGCT TCCAACAATC TTTACTCGGA CTCCACCTAT 
CTGTTCACTC TGGAGATCAA CAAACTGTCA AGGACAGCAT CGACCAGTGT CACCATCCTT 
TCCAAGTCTG GCAATCCTCC AACGATCAGT CTCGATGAAT CCCTTCCTGA GAAGATAAAG 
AGCAGTAGTT CTCATACGCT ACGAGTATTG ATCAGCCATA CATCAGATAT TACCATTGAC 
TGGGCTACTG TCGACATAGA TCTTGGATAT GGATACGTTG ACTTTTCACA GCTGAATAGT 
CAGTCATTCC TTATACAAGG AGATACATCA CCCTTCATCA CATACGCCTT TGTAACTATC 
CCCACAGGAA CTTTGGAAGA GGATGTTGCC TACTCTTTCT CAGTGACGGT AACTGATACC 
GAAGGACAGG TGTCAGCTAG TAAATTGACT ACCAACACGT ATGGAGGAGT ATCATCCTGT 
ACATTCAGTT TGGGTGATGG TTCAACCAGC TATCAAGAAC TTGACGAGAT CACTCTCCTA 
ACTCAGAACT GCGTGACTGA TGAAGAAGCT TACCCTCTGT CTTACCAAGT GTTCCGTGAG 
AAGGTCGAAT CATCTGTCAC CCGTTACAAC GCGATGACGG GTGCATTGGC AGAAGCGAGC 
AGCACTATAT TGGGTCTGCC GTCCTTTAGC GACAACCAGA ACACATTTGT TGTCAAGGTG 
TGCAATGATT TTGGAAGCTG TTCCTCGTAC AGTCTTACAT TGGACGTCAC TCCCATCGCA 
TCTTTCAGTC AAGAACAGTT TGATGAAAGT ATAACCAATC TGGTAGAACC ACAAAAGCTC 
TCAGGGAATT TCCTAGATGC GCTGGTAAAC TTCAACACCA TCACGTCAGT GGGTGTCGAC 
AGCACGAGTA GGAAACGGAG AGCAGTCTCG GAGACATCCA GCACAGCTAC TGAGCAGCTG 
GATTTACTTC AGAATGCCAT CTCGTCGACG GTCCTTGACA CGGCCACCGC CCAGACTCTG 
CTTGATCAGT CGGATGGCGT GGTCATTGCT GATCTCACAC TGTCTGATAT GGACATCTAC 
CTGGATATTT TAATTGAGCT CGTAGCTGTT TTCAGTAATG AAGGGCTTCC AGAGTCTAGC 
GCCGAGATCG TTCTTACAAA GACAAGCGAG ATCGCTGCTG AACTCGACCC TCAATACAAC 
TCGGACATGC TTGCCAAAAT TACGACCCTG AGAAACACAT TAATCCAGGG CCAGCTGAGT 
CAGATCCCAC TTGGAGGGGA CCCTACGGAG ACCAGGTCTG GTTCCGTTAC CACCAGGGCC 
TTCTATGCTA TCCCTAGTGA TACCCTCAGT ACTGCATCCA GCAACACCTC CTTCAACGTC 
AAGTTCGGAA GCGAGATCGA AAGCTTATAT GGTGCAAGTT GGTCGTGTGC TTCTGGAAGC 
ACTTGTTCTG GTGTTCAGAT CATGTTTACT CAGTACGACG ACGAGGTTGA TTACTACTCG 
ACTACGGACG AGGATAAGGC GAACAGAGCA TCTTCTATCC TTGAAATCGA TCTGCTTGAC 
CCGAGTGACA ATTCTGAGTT GAGTATTACT GGACTGAGCA GCCCCATATC AATCAACATG 
ACCGCTTCAC GCCTCCAATC CGACAAAGTC TATGACTGCT ATTGGTGGGA CACTAGCAGC 
AGCTCTTGGA GCCAGGATGG ACTTACGACC GTCTACCACT CATCGGTCTT GTCTGAGTGC 
CAAACTACTC ATCTTTCAGC CTTCACCCTG ATCGCTGTTG ATTCTCCTAC TACGATTATG 
ATGGCTACCA CGATAGTTGA TGACGCCAGT ACTTCTACCG ATGCAGTAGC CTCGACTGCG 
ACAACGGAAA TCAGTGAGAG CGGCAGCAGT GGTCTAAGTT CTACCGTCAT CATCGTCATT 
GCCGTGGTCA TATCTGTCGT TTTGATCCTA ATTATTATCA TCGCCATCGT GGTCGTGATG 
TCGAAGAAGT CCTCCAAGGT CGGTAATGCT GATATCGAGA ACTCGAACGG CAATGGTGCC 
AGGAATGGGA CCAGCACCGC CGATGGACTC GCTCCTGTTG AATCTGCGAA CCAGGCATTC 
AATGCCCCAC CAACAGCACC GGCCCACCCA ACCCATACTG TTCAAGTGGT CGCTGCCCCA 
GCTTCGGCAC CTGCTTCTCA AGAGCCTATC ATGGACGTTG CAGACCTTCC TGGATCCCCA 
TCACCCCCTC CTGATGGACC CCATATTGAT ACCATCATAA AGGATGTTCA GAATGATAGT 
AGGGCCCAAA CTCCAATGGA TAGAGCCATG ACGCCCGTTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011671997.1
CDS	199..10023
Translation	MKALVVVVIFSCLVAGAARAEESTEWQVEWTTEPAYNLSIIEPFTVPGYLLPDLNISDIISTVGMMYQAEDLVHAIGSLCVEMGPKLSWLTGGAMRELCDPVLEALRNGSYAEAYSICQSSWSFLETLIGGEEGGLEGEGEIDHGPIRRKRWGLSVPSIELPPLPSSPIQAVLNITVPFSPIENLDFNWTNSLFDDTANMVENIGLIPDLNRNWSFPIFDGTGIEVDFPYPEFSDKYGICSSLAGLISYNQPSSPSHGSIFGLVLNVIVAEQVRSIPGKCLSFFDGEEGSGDIDDDPSFKHEPDTMIWAFATLGGFDDIEQICNVSSWALLMRDEGKFELFNELVNQITTSIYSVISSRSRCIEFADSLHANIGLAPGNYSHVTSSEALCDEIMNFPDWRGNMSGIDFYNYDSMDEKDAFIYLLSIVEILSHKSDMASLEFLCRMSPYADEMFCTSLAYNDYRDVSREDIIEHCLRYVAFDGFEFDFLALLNAIGAEFDFDFYDFDQTCSHLTDIVFNSVIRQQFTIDNMIQKGMKAIGRVLSPIFCDLHSFRNGSLDSLEAPCGTPGRGCFNWTRPGEDYFFDRLDEGFIVGTIFSYLGGYRDGDHICHQMAFALDDETVLDMVAGKLRDSVYRLFDDVPFCSDTIGLIEKLIGVDEHAAQMIGFNSTDAFCGEISKIYHQGGNYNFSFVPLLDLFTNHSHGLEHFVTSESIMHVTNEMMKFAAMIMHSDNIGDAISKSCYMWQDSGPGITMPLFEVDDLCQVVLALNPAELQDVCIHDIIPWIEDLFDHHHDDEERGEENEIDFDFTGILETVGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFYDLPASHLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALEDDAQLDMVANKMKDSIFRFFNDPSFCPVTMDLINRLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSLEPLYVLFANVEEEGEFDPAAIINATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEVDDLCKTVLAFNSAELQDVCIHELFPWIEDVFDHHDDDEMREEENEIDFDFTGILETVGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFHDHPDTVLEAPCATPGHNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMVFALEDDAQLDMVANKMKGSIFRFFNDPSFCPVTMDIINRLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSFEPLYVLFANVEEEGEFDPAAIINATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEIDDLCQLVLEDNSDAVYQVCMGELFPLIESILEDEMYDFNDNHTNTVVDLSNIEICLDNGEVTFEALENLDILAYLLQTTHKLYNLTVFDESNICRAVTSVVESRNDIPTLISEFAVEMMSLFMPLGAEICSCWDQVFDDLFSDSDDSEFGVNQFNEIVRIAVNSIGLDSRHELCDHLIHLKPDNMSDYAQTVIEEILGFTADPEKCACTANRVIDFILPLVNVSMEEINNYLYQYLGYHSVDHVCSFVAQSFSGVIVDPSSINCNSQQEPTTKATTTTQSSLDNNGNSTQSSIDNTGNSTQSPIDNTGDSNETSTDDETCLFITDCAGVCNGNASLDCARECGGSAALDCAFKCGGSATTNECGWCVGGATERPSTLGFDQCGSCIGEGTAVIDCNGDCDGTAYRDACQVCVGGNTSVAEDVSDRFLDCLGICNGSFVTNDCDECVLPFSDGTIFSTADCNGDCNGFADINECSYCVGGNTGNSVDTGLSACGVCNTTIEPDFCLGCDGVADSGLVTDACDVCDGDGSTCFTVDNIAPSIIPANTDYQVKLRGAGFQDATTKQCKFLDSSGEQVSVVDISDEVDDEFNCTANLPQGTYSVYLSTESTELSEAFVTLYVYSEVIINSLSQSEFDIDDSVISISVNMVSTSGAFTAYKDLTTPKAIVFTETERLYYEGTFGGDNDELLEFTGPMLTTSAQVVVYPSFNGIDYLVTPDDAGFTITYYAPAPVAQSLKFSPTGHLLELEFDVAINEDSVTSCDAVFMNTTSLGGAAAQCFAFPYLKTIYILPFSSASVNLIGPDDELVLRADVLKKRDEEYARYASGTLTASSPDEAVTVYAALTGTEAISSCGNVRLSALQSFGNAGRFFTYQWSVTSGGSIPSGLTDDLTAAAESAYVTLDGSYLDADVPYTFSMTAENFLGGSDTKTLEVTRSALNKPDVVIYPERVDPNSALISQAFYLRAAVRFYEACGGAEQMEYAWSVDDDSIALNLQTINKPLLYVAAGSLPGDSQVTFTLEVNRESDPSTVTTQTVTITTVYTDLDALIDGGSERTIGVDSESFTLDGSSSFDPDSENEEMTYLWTCQKSNTETGSYEPCVSSETREVFPAEADSKTSTLTLASNNLYSDSTYLFTLEINKLSRTASTSVTILSKSGNPPTISLDESLPEKIKSSSSHTLRVLISHTSDITIDWATVDIDLGYGYVDFSQLNSQSFLIQGDTSPFITYAFVTIPTGTLEEDVAYSFSVTVTDTEGQVSASKLTTNTYGGVSSCTFSLGDGSTSYQELDEITLLTQNCVTDEEAYPLSYQVFREKVESSVTRYNAMTGALAEASSTILGLPSFSDNQNTFVVKVCNDFGSCSSYSLTLDVTPIASFSQEQFDESITNLVEPQKLSGNFLDALVNFNTITSVGVDSTSRKRRAVSETSSTATEQLDLLQNAISSTVLDTATAQTLLDQSDGVVIADLTLSDMDIYLDILIELVAVFSNEGLPESSAEIVLTKTSEIAAELDPQYNSDMLAKITTLRNTLIQGQLSQIPLGGDPTETRSGSVTTRAFYAIPSDTLSTASSNTSFNVKFGSEIESLYGASWSCASGSTCSGVQIMFTQYDDEVDYYSTTDEDKANRASSILEIDLLDPSDNSELSITGLSSPISINMTASRLQSDKVYDCYWWDTSSSSWSQDGLTTVYHSSVLSECQTTHLSAFTLIAVDSPTTIMMATTIVDDASTSTDAVASTATTEISESGSSGLSSTVIIVIAVVISVVLILIIIIAIVVVMSKKSSKVGNADIENSNGNGARNGTSTADGLAPVESANQAFNAPPTAPAHPTHTVQVVAAPASAPASQEPIMDVADLPGSPSPPPDGPHIDTIIKDVQNDSRAQTPMDRAMTPVE

Target ORF information:

RefSeq Version	XM_011673695.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011673695.1

ORF Insert Sequence:

ATGAAGGCAC TTGTGGTGGT TGTCATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCATC 
ATCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATGG GACCCAAGCT TAGCTGGCTT ACCGGTGGAG CAATGCGAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAGT 
TCTTGGTCCT TCCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCAATCGAAA ATTTGGATTT TAACTGGACC AACTCTTTAT TCGATGATAC TGCGAATATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGAA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGATTGG TTCTAAACGT CATCGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG ATACAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGAAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATGTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAA TGAATTTCCC CGATTGGAGA 
GGCAATATGA GCGGGATTGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGACTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GAATGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AACTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGGTTGACGA TTTATGTAAA 
ACAGTATTGG CCTTTAATTC TGCAGAACTC CAAGATGTCT GCATCCACGA ACTATTTCCC 
TGGATTGAGG ACGTTTTTGA CCACCATGAT GATGATGAAA TGAGGGAAGA AGAAAACGAA 
ATTGACTTTG ATTTCACAGG GATTCTCGAA ACGGTAGGCG AGGAATTCGG ATTCGACTTT 
CACGATTCTC ACCAAAGTTG CTCTCATCTT ACTGGTATTC TCTTTGATGA TACCGTCAGC 
CAAAACTTTC CAATCGACAG CGTTGTGGTA AGGGGAATAA GAGCTGTTGG TAAGATAGTA 
TCACCCATAT TCTGTGATTT TGAATCATTT CATGACCACC CAGATACTGT CTTGGAAGCT 
CCGTGTGCAA CTCCTGGACA TAACTGCTTT GATTGGTCAA GCCGAGGAGA AGACTTCTTC 
TATGAGCGGG AAGACATTGG CTTTGTTATT GGTACAATTT TCAGTTATAT TGGAGGCTAC 
AGAGATGGGG ACCACCTTTG TCACCAAATG GTATTTGCAC TGGAGGATGA TGCACAACTT 
GACATGGTCG CAAATAAAAT GAAGGGTAGT ATCTTTCGAT TCTTCAACGA CCCGTCATTC 
TGTCCTGTGA CGATGGACAT TATCAACAGG CTCATCGGAT TTGACGAGGA TGCTGCAAGT 
TTACTCGGCT TTGAGAATGC GGATGCCTTC TGCGGAAAGG TCAATCAAAT ATACAATGAA 
GATGGCGATT ATAACGTCAG TTTTGAGCCT CTCTATGTTT TGTTTGCCAA TGTTGAAGAA 
GAAGGAGAAT TTGACCCTGC AGCCATTATA AATGCCACAA CCGAGATGAT TAAATTCGCC 
GCCATGATCA TGCGTTCAGA CCACATTGGA GATGCCATTT CTAAAACATG CTACGTGTGG 
CAGGAATCTG CTGCAATGTT AAATATTTCA GAGATTGATG ATTTATGTCA ATTGGTTCTT 
GAGGACAATT CCGATGCTGT TTACCAAGTC TGCATGGGTG AACTTTTCCC ACTTATTGAG 
TCAATTCTAG AAGACGAGAT GTATGATTTC AATGATAATC ATACAAACAC CGTTGTAGAT 
TTATCGAACA TTGAGATCTG TCTCGATAAT GGCGAGGTGA CTTTTGAGGC ACTTGAAAAT 
TTAGATATAT TGGCATATCT ACTACAAACC ACACACAAGC TCTATAATTT AACCGTGTTC 
GACGAGAGCA ACATTTGTAG AGCAGTGACC AGCGTAGTAG AATCTAGAAA TGACATTCCG 
ACACTGATAT CTGAATTTGC TGTGGAAATG ATGTCCCTCT TCATGCCGCT TGGTGCAGAA 
ATATGCTCTT GTTGGGATCA AGTTTTTGAT GATCTCTTCT CGGACTCCGA CGACTCGGAA 
TTTGGCGTAA ACCAATTCAA TGAGATAGTC CGGATTGCTG TGAACAGTAT TGGATTAGAC 
AGTCGTCATG AACTCTGTGA CCATCTCATT CATCTAAAGC CGGACAACAT GTCTGATTAC 
GCACAAACGG TGATTGAGGA AATCCTTGGT TTTACGGCTG ATCCTGAGAA GTGTGCCTGT 
ACCGCCAATC GTGTCATCGA TTTCATCCTT CCCTTGGTTA ATGTTTCAAT GGAGGAAATC 
AATAACTACC TTTACCAGTA CCTGGGATAC CATTCTGTGG ATCACGTGTG TAGTTTCGTG 
GCCCAATCCT TCTCGGGAGT AATAGTCGAC CCTTCAAGCA TAAACTGTAA CAGCCAACAA 
GAACCAACAA CCAAAGCTAC GACCACAACT CAATCATCAT TAGACAATAA TGGAAACTCA 
ACTCAATCAT CAATAGACAA TACTGGAAAC TCAACTCAAT CACCAATAGA CAATACTGGA 
GACTCGAATG AAACTTCGAC TGACGACGAA ACCTGTCTCT TCATCACTGA CTGCGCCGGT 
GTATGCAACG GTAACGCTAG TTTGGATTGC GCCCGAGAAT GCGGCGGCAG CGCGGCACTC 
GACTGCGCCT TCAAGTGCGG TGGCTCTGCG ACCACGAACG AATGTGGATG GTGCGTCGGT 
GGAGCTACGG AAAGACCAAG CACTTTAGGT TTCGACCAGT GTGGTTCCTG CATTGGGGAA 
GGCACTGCGG TCATCGACTG CAATGGTGAC TGTGACGGTA CTGCTTACCG CGATGCCTGT 
CAAGTCTGCG TAGGTGGCAA TACGAGTGTG GCTGAGGATG TAAGCGATCG TTTCTTGGAC 
TGCCTAGGTA TCTGTAATGG TAGTTTTGTG ACCAATGATT GTGATGAATG CGTGCTGCCA 
TTCAGCGATG GGACTATTTT CTCCACGGCG GATTGTAACG GTGACTGCAA TGGATTTGCC 
GACATAAATG AGTGTAGTTA CTGTGTTGGG GGTAATACCG GTAACTCTGT GGATACTGGA 
TTGTCTGCAT GTGGAGTCTG CAATACTACG ATCGAACCAG ACTTCTGCCT CGGCTGTGAT 
GGCGTCGCTG ATAGTGGCCT AGTGACCGAT GCGTGTGATG TATGCGATGG GGATGGTAGC 
ACTTGTTTCA CCGTGGACAA TATAGCTCCC TCTATCATCC CAGCTAACAC CGACTATCAG 
GTTAAACTTC GTGGTGCCGG ATTCCAAGAT GCGACTACCA AACAGTGTAA GTTCCTGGAT 
AGCAGCGGTG AGCAGGTCAG CGTGGTCGAT ATCAGCGATG AGGTGGATGA CGAGTTCAAC 
TGCACCGCTA ATCTTCCTCA AGGAACGTAT TCTGTTTACC TTTCGACGGA GTCAACGGAA 
CTATCAGAAG CATTCGTCAC CCTCTATGTC TATTCAGAAG TTATAATAAA CAGCTTATCC 
CAGAGCGAGT TTGATATAGA TGATAGTGTG ATTTCCATCT CTGTCAACAT GGTATCAACA 
AGCGGAGCCT TTACGGCATA CAAGGACTTG ACCACTCCAA AAGCTATCGT CTTCACAGAG 
ACCGAGAGAC TTTACTACGA AGGAACTTTT GGAGGAGACA ACGACGAGCT TCTCGAGTTT 
ACGGGTCCGA TGCTGACTAC CTCGGCTCAA GTTGTCGTTT ACCCGTCCTT CAACGGAATC 
GACTACCTGG TCACCCCCGA TGATGCTGGT TTCACGATCA CCTACTACGC CCCTGCACCG 
GTCGCTCAGA GTCTTAAATT CTCCCCTACT GGACATCTTC TGGAACTCGA GTTCGACGTC 
GCAATCAACG AAGACTCGGT GACATCATGT GATGCAGTAT TCATGAATAC CACCAGCCTG 
GGTGGTGCTG CTGCCCAGTG CTTTGCATTC CCTTACCTAA AAACTATCTA TATACTACCC 
TTCAGTTCGG CCAGTGTAAA TCTTATTGGA CCAGATGATG AGCTAGTGCT GCGCGCTGAC 
GTTCTGAAAA AACGTGATGA GGAATACGCT CGGTACGCCA GCGGTACTCT TACCGCATCC 
TCCCCCGACG AGGCGGTAAC CGTCTACGCG GCTCTGACCG GTACAGAAGC AATTAGCAGC 
TGCGGTAATG TACGACTTAG TGCTCTGCAA TCCTTTGGAA ACGCAGGACG TTTTTTCACC 
TATCAGTGGT CTGTGACCAG TGGCGGTTCG ATCCCATCTG GCTTGACGGA TGATCTAACA 
GCCGCAGCGG AGTCCGCCTA TGTCACTCTC GATGGATCCT ACCTCGACGC GGACGTGCCG 
TATACATTCA GCATGACAGC AGAGAACTTC CTCGGAGGCA GTGACACAAA GACCTTGGAG 
GTGACTCGGT CTGCTCTCAA CAAACCAGAC GTCGTGATCT ACCCGGAAAG AGTTGATCCT 
AATAGTGCCC TTATCTCGCA AGCGTTCTAC CTCCGCGCTG CTGTACGATT CTACGAGGCC 
TGCGGTGGAG CTGAGCAGAT GGAGTATGCC TGGTCTGTAG ATGATGATAG TATTGCTCTT 
AACCTACAAA CCATAAACAA ACCACTCCTG TATGTGGCAG CTGGTTCTTT ACCAGGCGAC 
TCCCAAGTGA CCTTCACACT GGAGGTCAAC CGGGAAAGTG ATCCCAGCAC GGTGACCACG 
CAGACCGTGA CCATTACCAC GGTCTACACC GACCTCGACG CCCTAATCGA TGGCGGGAGT 
GAGCGGACCA TCGGCGTCGA CAGTGAGTCG TTCACACTGG ATGGATCGTC GTCGTTTGAT 
CCCGACAGTG AAAATGAAGA GATGACCTAC CTCTGGACTT GTCAGAAGTC AAACACTGAG 
ACAGGCAGCT ACGAGCCTTG TGTATCAAGC GAGACACGTG AGGTGTTCCC GGCGGAAGCT 
GACTCCAAAA CAAGTACTCT CACGTTAGCT TCCAACAATC TTTACTCGGA CTCCACCTAT 
CTGTTCACTC TGGAGATCAA CAAACTGTCA AGGACAGCAT CGACCAGTGT CACCATCCTT 
TCCAAGTCTG GCAATCCTCC AACGATCAGT CTCGATGAAT CCCTTCCTGA GAAGATAAAG 
AGCAGTAGTT CTCATACGCT ACGAGTATTG ATCAGCCATA CATCAGATAT TACCATTGAC 
TGGGCTACTG TCGACATAGA TCTTGGATAT GGATACGTTG ACTTTTCACA GCTGAATAGT 
CAGTCATTCC TTATACAAGG AGATACATCA CCCTTCATCA CATACGCCTT TGTAACTATC 
CCCACAGGAA CTTTGGAAGA GGATGTTGCC TACTCTTTCT CAGTGACGGT AACTGATACC 
GAAGGACAGG TGTCAGCTAG TAAATTGACT ACCAACACGT ATGGAGGAGT ATCATCCTGT 
ACATTCAGTT TGGGTGATGG TTCAACCAGC TATCAAGAAC TTGACGAGAT CACTCTCCTA 
ACTCAGAACT GCGTGACTGA TGAAGAAGCT TACCCTCTGT CTTACCAAGT GTTCCGTGAG 
AAGGTCGAAT CATCTGTCAC CCGTTACAAC GCGATGACGG GTGCATTGGC AGAAGCGAGC 
AGCACTATAT TGGGTCTGCC GTCCTTTAGC GACAACCAGA ACACATTTGT TGTCAAGGTG 
TGCAATGATT TTGGAAGCTG TTCCTCGTAC AGTCTTACAT TGGACGTCAC TCCCATCGCA 
TCTTTCAGTC AAGAACAGTT TGATGAAAGT ATAACCAATC TGGTAGAACC ACAAAAGCTC 
TCAGGGAATT TCCTAGATGC GCTGGTAAAC TTCAACACCA TCACGTCAGT GGGTGTCGAC 
AGCACGAGTA GGAAACGGAG AGCAGTCTCG GAGACATCCA GCACAGCTAC TGAGCAGCTG 
GATTTACTTC AGAATGCCAT CTCGTCGACG GTCCTTGACA CGGCCACCGC CCAGACTCTG 
CTTGATCAGT CGGATGGCGT GGTCATTGCT GATCTCACAC TGTCTGATAT GGACATCTAC 
CTGGATATTT TAATTGAGCT CGTAGCTGTT TTCAGTAATG AAGGGCTTCC AGAGTCTAGC 
GCCGAGATCG TTCTTACAAA GACAAGCGAG ATCGCTGCTG AACTCGACCC TCAATACAAC 
TCGGACATGC TTGCCAAAAT TACGACCCTG AGAAACACAT TAATCCAGGG CCAGCTGAGT 
CAGATCCCAC TTGGAGGGGA CCCTACGGAG ACCAGGTCTG GTTCCGTTAC CACCAGGGCC 
TTCTATGCTA TCCCTAGTGA TACCCTCAGT ACTGCATCCA GCAACACCTC CTTCAACGTC 
AAGTTCGGAA GCGAGATCGA AAGCTTATAT GGTGCAAGTT GGTCGTGTGC TTCTGGAAGC 
ACTTGTTCTG GTGTTCAGAT CATGTTTACT CAGTACGACG ACGAGGTTGA TTACTACTCG 
ACTACGGACG AGGATAAGGC GAACAGAGCA TCTTCTATCC TTGAAATCGA TCTGCTTGAC 
CCGAGTGACA ATTCTGAGTT GAGTATTACT GGACTGAGCA GCCCCATATC AATCAACATG 
ACCGCTTCAC GCCTCCAATC CGACAAAGTC TATGACTGCT ATTGGTGGGA CACTAGCAGC 
AGCTCTTGGA GCCAGGATGG ACTTACGACC GTCTACCACT CATCGGTCTT GTCTGAGTGC 
CAAACTACTC ATCTTTCAGC CTTCACCCTG ATCGCTGTTG ATTCTCCTAC TACGATTATG 
ATGGCTACCA CGATAGTTGA TGACGCCAGT ACTTCTACCG ATGCAGTAGC CTCGACTGCG 
ACAACGGAAA TCAGTGAGAG CGGCAGCAGT GGTCTAAGTT CTACCGTCAT CATCGTCATT 
GCCGTGGTCA TATCTGTCGT TTTGATCCTA ATTATTATCA TCGCCATCGT GGTCGTGATG 
TCGAAGAAGT CCTCCAAGGT CGGTAATGCT GATATCGAGA ACTCGAACGG CAATGGTGCC 
AGGAATGGGA CCAGCACCGC CGATGGACTC GCTCCTGTTG AATCTGCGAA CCAGGCATTC 
AATGCCCCAC CAACAGCACC GGCCCACCCA ACCCATACTG TTCAAGTGGT CGCTGCCCCA 
GCTTCGGCAC CTGCTTCTCA AGAGCCTATC ATGGACGTTG CAGACCTTCC TGGATCCCCA 
TCACCCCCTC CTGATGGACC CCATATTGAT ACCATCATAA AGGATGTTCA GAATGATAGT 
AGGGCCCAAA CTCCAATGGA TAGAGCCATG ACGCCCGTTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REJ8 ( XM_030976261.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030832121.1 Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X1, mRNA.

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CloneID	OSd71217
Clone ID Related Accession (Same CDS sequence)	XM_030976261.1 , XM_030976262.1
Accession Version	XM_030976261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	receptor for egg jelly 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145615.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC TTGTGGTGGT TGTAATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCCTC 
GTCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATAG GGCCAAAGCT TAGCTGGCTT TCCGATGGAG CAATGCAAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAAT 
TCTTGGTCCT TTCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCACTCGAAG ATTTGGATTT TAACTGGACC AACCCTTTAT TCGATGATAC TGCGAACATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGTA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGAGTGG TTCTAAACGT CATTGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG GTGCAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGGAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATTTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAC TGGATTCTCT CTATTGGAGA 
GGCAATATGA GCGAGATCGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGAGTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GACTGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AATTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGGTTGACGA TTTATGTAAA 
ACAGTATTGG CCTTTAATTC TGCAGAACTC CAAGATGTCT GCATCCACGA ACTATTTCCG 
TGGATTGAGG ATGTTTTTGA CCACCATGAG GATGATGAAA TGAGGGAAGA AGAAAACGAA 
ATTGACTTTG ATTTCACAGG GATTCTCGAA ACGGTAGGCG AGGAATTCGG ATTCGACTTT 
CACGATTCTC ACCAAAGTTG CTCTCATCTT ACTGGTATTC TCTTTGATGA TACCGTCAGC 
CAAAACTTTC CAATCGACAG CGTTGTGGTA AGGGGAATAA GAGCTGTTGG TAAGATAGTA 
TCACCCATAT TCTGTGATTT TGAATCATTT CATGACCACC CAGATACTGT CTTGGAAGCT 
CCGTGTGCAA CTCCTGGACA AAACTGCTTT GATTGGTCAA GCCGAGGAGA AGACTTCTTC 
TATGAGCGAG AAGACATTGG CTTTGTCATT GGTACAATTT TCAGTTATAT CGGAGGCTAC 
AGAGATGGGG ACCACCTTTG TCACCAAATG GCCTTTGCAC TGGATGATGA TGAACAACTT 
GACATGGTAG CAAATAAAAT GAAGGATAGT ATCTTCCGAT TCTTCAACGA CCCATCATTC 
TGTCCTGCGA CGATGGACAT TATCAACAAG CTCATCGGAT TTGACGAGGA TGCTGCAAGT 
TTACTCGGCT TTGAGAATGC GGATGCCTTC TGCGGGAAGG TCAATCAAAT ATACAATGAA 
GATGGCGATT ATAACGTCAG TTTGGAACCT CTCTATGTTT TGTTTGCCGA TGTTGAAGAA 
GAAGGAAACT TTGACCCTGC AGCCATCATG AATGCCACAA CCGAGATGAT TAAATTCGCC 
GCCATGATCA TGCGTTCAGA CCACATTGGA GATGCCATTT CTAAAACATG CTACGTGTGG 
CAGGAATCTG CTGCAATGTT AAATATTTCA GAGGTTGACG ATTTATGTCA AACAGTATTG 
GCCTTTAATT CTGCAGAACT CCAAGATGTC TGCATCCACG AACTATTTCC CTGGATTGAG 
GACGTTTTTG ACCACCATGA TGATGATGAA ATGAGGGAAG AAGAAAACGA AATTGACTTT 
GATTTCACAG GGATTCTCGA AACGGTAGGC GAGGAATTCG GATTCGACTT ACACGATTCT 
CACCAAAGTT GCTCTCATCT TACTGGTATT CTCTTTGATG ATACCGTCAG CCAAAACTTT 
CCAATCGACA GCGTTGTAGT AAGGGGAATA AGAGCTGTTG GTAAGATAGT ATCACCCATA 
TTCTGTGATT TTGAATCATT TCATGACCAC CCAGATACTG TCTTGGAAGC TCCGTGTGCA 
ACTCCTGGAC AAAACTGCTT TGATTGGTCA AGCCGAGGAG AAGACTTCTT CTATGAGCGG 
GAAGACATTG GCTTTGTCAT TGGTACAATT TTCAGTTATA TTGGGGGCTA CAGAGATGGG 
GACCACCTTT GTCACCAAAT GGCCTTTGCA CTGGAGGATG ATGCACAACT TGACATGGTC 
GCAAATAAAA TGAAGGATAG TATCTTTCGA TTCTTCAACG ACCCGTCATT CTGTCCTGTG 
ACGATGGACA TTATTAACAG GCTCATCGGA TTTGACGAGG ATGCTGCAAG TTTACTCGGC 
TTTGAGAATG CGGATGCCTT CTGCGGAAAG GTCAATCAAA TATACAATGA AGATGGCGAT 
TATAACGTCA GTTTTGAGCC TCTCTATGTT TTGTTTGCCA ATGTTGAAGA AGAAGGAGAA 
TTTGACCCTG CAGCCATTAT AAATGCCACA ACCGAGATGA TTAAATTCGC CGCCATGATC 
ATGCGTTCAG ACCACATTGG AGATGCCATT TCTAAAACAT GCTACGTGTG GCAGGAATCT 
GCTGCAATGT TAAATATTTC AGAGATTGAT GATTTATGTC AATTGGTTCT TGAGGACAAT 
TCCGATGCTG TTTACCAAGT CTGCATGGGT GAACTTTTCC CACTTATTGA GTCAATTCTA 
GAAGACGAGA TGTATGATTT CAATGATAAT CATACAAACA CCGTTGTAGA TTTATCGAAC 
ATTGAGATCT GTCTCGATAA TGGCGAGGTG ACTTTTGAGG CACTTGAAAA TTTAGATATA 
TTGGCATATC TACTACAAAC CACACACAAG CTCTATAATT TAACCGTGTT CGACGAGAGC 
AACATTTGTA GAGCAGTGAC CAGCGTAGTA GAATCTAGAA ATGACATTCC GACACTGATA 
TCTGAATTTG CTGTGGAAAT GATGTCCCTC TTCATGCCGC TTGGTGCAGA AATATGCTCT 
TGTTGGGATC AAGTTTTTGA TGATCTCTTC TCGGACTCCG ACGACTCGGA ATTTGGCGTA 
AACCAATTCA ATGAGATAGT CCGGATTGCT GTGAACAGTA TTGGATTAGA CAGTCGTCAT 
GAACTCTGTG ACCATCTCAT TCATCTAAAG CCGGACAACA TGTCTGATTA CGCACAAACG 
GTGATTGAGG AAATCCTTGG TTTTACGGCT GATCCTGAGA AGTGTGCCTG TACCGCCAAT 
CGTGTCATCG ATTTCATCCT TCCCTTGGTT AATGTTTCAA TGGAGGAAAT CAATAACTAC 
CTTTACCAGT ACCTGGGATA CCATTCTGTG GATCACGTGT GTAGTTTCGT GGCCCAATCC 
TTCTCGGGAG TAATAGTCGA CCCTTCAAGC ATAAACTGTA ACAGCCAACA AGAACCAACA 
ACCAAAGCTA CGACCACAAC TCAATCATCA TTAGACAATA ATGGAAACTC AACTCAATCA 
TCAATAGACA ATACTGGAAA CTCAACTCAA TCACCAATAG ACAATACTGG AGACTCGAAT 
GAAACTTCGA CTGACGACGA AACCTGTCTC TTCATCACTG ACTGCGCCGG TGTATGCAAC 
GGTAACGCTA GTTTGGATTG CGCCCGAGAA TGCGGCGGCA GCGCGGCACT CGACTGCGCC 
TTCAAGTGCG GTGGCTCTGC GACCACGAAC GAATGTGGAT GGTGCGTCGG TGGAGCTACG 
GAAAGACCAA GCACTTTAGG TTTCGACCAG TGTGGTTCCT GCATTGGGGA AGGCACTGCG 
GTCATCGACT GCAATGGTGA CTGTGACGGT ACTGCTTACC GCGATGCCTG TCAAGTCTGC 
GTAGGTGGCA ATACGAGTGT GGCTGAGGAT GTAAGCGATC GTTTCTTGGA CTGCCTAGGT 
ATCTGTAATG GTAGTTTTGT GACCAATGAT TGTGATGAAT GCGTGCTGCC ATTCAGCGAT 
GGGACTATTT TCTCCACGGC GGATTGTAAC GGTGACTGCA ATGGATTTGC CGACATAAAT 
GAGTGTAGTT ACTGTGTTGG GGGTAATACC GGTAACTCTG TGGATACTGG ATTGTCTGCA 
TGTGGAGTCT GCAATACTAC GATCGAACCA GACTTCTGCC TCGGCTGTGA TGGCGTCGCT 
GATAGTGGCC TAGTGACCGA TGCGTGTGAT GTATGCGATG GGGATGGTAG CACTTGTTTC 
ACCGTGGACA ATATAGCTCC CTCTATCATC CCAGCTAACA CCGACTATCA GGTTAAACTT 
CGTGGTGCCG GATTCCAAGA TGCGACTACC AAACAGTGTA AGTTCCTGGA TAGCAGCGGT 
GAGCAGGTCA GCGTGGTCGA TATCAGCGAT GAGGTGGATG ACGAGTTCAA CTGCACCGCT 
AATCTTCCTC AAGGAACGTA TTCTGTTTAC CTTTCGACGG AGTCAACGGA ACTATCAGAA 
GCATTCGTCA CCCTCTATGT CTATTCAGAA GTTATAATAA ACAGCTTATC CCAGAGCGAG 
TTTGATATAG ATGATAGTGT GATTTCCATC TCTGTCAACA TGGTATCAAC AAGCGGAGCC 
TTTACGGCAT ACAAGGACTT GACCACTCCA AAAGCTATCG TCTTCACAGA GACCGAGAGA 
CTTTACTACG AAGGAACTTT TGGAGGAGAC AACGACGAGC TTCTCGAGTT TACGGGTCCG 
ATGCTGACTA CCTCGGCTCA AGTTGTCGTT TACCCGTCCT TCAACGGAAT CGACTACCTG 
GTCACCCCCG ATGATGCTGG TTTCACGATC ACCTACTACG CCCCTGCACC GGTCGCTCAG 
AGTCTTAAAT TCTCCCCTAC TGGACATCTT CTGGAACTCG AGTTCGACGT CGCAATCAAC 
GAAGACTCGG TGACATCATG TGATGCAGTA TTCATGAATA TCACCAGCCT GGGTGGTGCT 
GCTGCCCAGT GCTTTGCATT CCCTTACCTA AAAACTATCT ATATACTACC CTTCAGTTCG 
GCCAGTGTAA ATCTTATTGG ACCAGATGAT GAGCTAGTGC TGCGCGCTGA CGTTCTGAAA 
AAACGTGATG AGGAATACGC TCGGTACGCC AGCGGTACTC TTACCGCATC CTCCCCCGAC 
GAGGCGGTAA CCGTCTACGC GGCTCTGACC GGTACAGAAG CAATTAGCAG CTGCGGTAAT 
GTACGACTTA GTGCTCTGCA ATCCTTTGGA AACGCAGGAC GTTTTTTCAC CTATCAGTGG 
TCTGTGACCA GTGGCGGTTC GATCCCATCT GGCTTGACGG ATGATCTAAC AGCCGCAGCG 
GAGTCCGCCT ATGTCACTCT CGATGGATCC TACCTCGACG CGGACGTGCC GTATACATTC 
AGCATGACAG CAGAGAACTT CCTCGGAGGC AGTGACACAA AGACCTTGGA GGTGACTCGG 
TCTGCTCTCA ACAAACCAGA CGTCGTGATC TACCCGGAAA GAGTTGATCC TAATAGTGCC 
CTTATCTCGC AAGCGTTCTA CCTCCGCGCT GCTGTACGAT TCTACGAGGC CTGCGGTGGA 
GCTGAGCAGA TGGAGTATGC CTGGTCTGTA GATGATGATA GTATTGCTCT TAACCTACAA 
ACCATAAACA AACCACTCCT GTATGTGGCA GCTGGTTCTT TACCAGGCGA CTCCCAAGTG 
ACCTTCACAC TGGAGGTCAA CCGGGAAAGT GATCCCAGCA CGGTGACCAC GCAGACCGTG 
ACCATTACCA CGGTCTACAC CGACCTCGAC GCCCTAATCG ATGGCGGGAG TGAGCGGACC 
ATCGGCGTCG ACAGTGAGTC GTTCACACTG GATGGATCGT CGTCGTTTGA TCCCGACAGT 
GAAAATGAAG AGATGACCTA CCTCTGGACT TGTCAGAAGT CGAACACTGA GACAGGCAGC 
TACGAGCCTT GTGTATCAAG CGAGACACGT GAGGTGTTCC CGGCGGAAGC TGACTCCAAA 
ACAAGTACTC TCACGTTAGC TTCCAACAAT CTTTACTCGG ACTCCACCTA TCTGTTCACT 
CTGGAGATCA ACAAACTGTC AAGGACAGCA TCGACCAGTG TCACCATCCT TTCCAAGTCT 
GGCAATCCTC CAACGATCAG TCTCGATGAA TCCCTTCCTG AGAAGATAAA GAGCAGTAGT 
TCTCATACGC TACGAGTATT GATCAGCCAT ACATCAGATA TTACCATTGA CTGGGCTACT 
GTCGACATAG ATCTTGGATA TGGATACGTT GACTTTTCAC AGCTGAATAG TCAGTCATTC 
CTTATACAAG GAGATACATC ACCCTTCATC ACATACGCCT TTGTAACTAT CCCCACAGGA 
ACTTTGGAAG AGGATGTTGC CTACTCTTTC TCAGTGACGG TAACTGATAC CGAAGGACAG 
GTGTCAGCTA GTAAATTGAC TACCAACACG TATGGAGGAG TATCATCCTG TACATTCAGT 
TTGGGTGATG GTTCAACCAG CTATCAAGAA CTTGACGAGA TCACTCTCCT AACTCAGAAC 
TGCGTGACTG ATGAAGAAGC TTACCCTCTG TCTTACCAAG TGTTCCGTGA GAAGGTCGAA 
TCATCTGTCA CCCGTTACAA CGCGATGACG GGTGCATTGG CAGAAGCGAG CAGCACTATA 
TTGGGTCTGC CGTCCTTTAG CGACAACCAG AACACATTTG TTGTCAAGGT GTGCAATGAT 
TTTGGAAGCT GTTCCTCGTA CAGTCTTACA TTGGACGTCA CTCCCATCGC ATCTTTCAGT 
CAAGAACAGT TTGATGAAAG TATAACCAAT CTGGTAGAAC CACAAAAGCT CTCAGGGAAT 
TTCCTAGATG CGCTGGTAAA CTTCAACACC ATCACGTCAG TGGGTGTCGA CAGCACGAGT 
AGGAAACGGA GAGCAGTCTC GGAGACATCC AGCACAGCTA CTGAGCAGCT GGATTTACTT 
CAGAATGCCA TCTCGTCGAC GGTCCTTGAC ACGGCCACCG CCCAGACTCT GCTTGATCAG 
TCGGATGGCG TGGTCATTGC TGATCTCACA CTGTCTGATA TGGACATATA CCTGGATATT 
TTAATTGAGC TCGTAGCTGT TTTCAGTGAT GAAGGGCTTC CAGAGTCTAG CGCCGAGATC 
GTTCTTACAA AGACAAGCGA GATCGCTGCT GAACTCGACC CTCAATACAA CTCGGACATG 
CTTGCCAAAA TTACGACCCT GAGAAACACA TTAATCCAGG GCCAGCTGAG TCAGATCCCA 
CTTGGAGGGG ACCCTACGGA GACCAGGTCT GGTTCCGTTA CCACCAGGGC CTTCTATGCT 
ATCCCTAGTG ATACCCTCAG TACTGCATCC AGCAACACCT CCTTCAACGT CAAGTTCGGA 
AGCGAGATCG AAAGCTTATA TGGTGCAAGT TGGTCGTGTG CTTCTGGAAG CACTTGTTCT 
GGTGTTCAGA TCATGTTTAC GCAGTACGAC GACGAGGTTG ATTACTACTC GACTACGGAC 
GAGGATAAGG CGAACAGAGC ATCTTCTATC CTTGAAATCG ATCTGCTTGA CCCGAGTGAC 
AATTCTGAGT TGAGTATTAC TGGACTGAGC AGCCCCATAT CAATCAACAT GACCGCTTCA 
CGCCTCCAAT CCGACAAAGT CTATGACTGC TATTGGTGGG ACACTAGCAG CAGCTCTTGG 
AGCCAGGATG GACTTACGAC CGTCTACCAC TCCTCGGTCT TGTCTGAGTG CCAAACTACT 
CATCTTTCAG CCTTCACCCT GATCGCTGTT GATTCTCCTA CTACGATTAT GATGGCTACC 
ACGATAGTTG ATGACGCCAG TACTTCTACC GATGCAGTAG CCTCGACTGC GACAACGGAA 
ATCAGTGAGA GCGGCAGCAG TGGTCTAAGT TCTACCGTCA TCATCGTCAT TGCCGTGGTC 
ATATCTGTCG TTTTGATCCT AATTATTATC ATCGCCATCG TGGTCGTGAT GTCGAAGAAG 
TCCTCCAAGG TCGGTAATGC TGATATCGAG AACTCGAACG GCAATGGTGC CAGGAATGGG 
ACCAGCACCG CCGATGGACT CGCTCCTGTT GAATCTGCGA ACCAGGCATT CAATGCCCCA 
CCAACAGCAC CGGCCCACCC AACCCATACT GTTCAAGTGG TCGCTGCCCC AGCTTCGGCA 
CCTGCTTCTC AAGAGCCTAT CATGGACGTT GCAGACCTTC CTGGATCCCC ATCACCCCCT 
CCTGATGGAC CCCATATTGA TACCATCATA AAGGATGTTC AGAATGATAG TAGGGCCCAA 
ACTCCAATGG ATAGAGCCAT GACGCCCGTT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030832121.1
CDS	310..11085
Translation	MKALVVVVIFSCLVAGAARAEESTEWQVEWTTEPAYNLSLVEPFTVPGYLLPDLNISDIISTVGMMYQAEDLVHAIGSLCVEIGPKLSWLSDGAMQELCDPVLEALRNGSYAEAYSICQNSWSFLETLIGGEEGGLEGEGEIDHGPIRRKRWGLSVPSIELPPLPSSPIQAVLNITVPFSPLEDLDFNWTNPLFDDTANMVENIGLIPDLNRNWSFPIFDGTGIEVDFPYPEFSDKYGICSSLAGLISYNQPSSPSHGSIFGVVLNVIVAEQVRSIPGKCLSFFDGEEGSGDIDDDPSFKHEPGAMIWAFATLGGFDDIEQICNVSSWALLMRDEGKFELFNELVNQITTSIYSVISSRSRCIEFADSLHANIGLAPGNYSHFTSSEALCDEILDSLYWRGNMSEIDFYNYDSMDEKDAFIYLLSIVEILSHKSDMASLEFLCRMSPYADEMFCTSLAYNDYRDVSREDIIEHCLRYVAFDGFEFDFLALLNAIGAEFEFDFYDFDQTCSHLTDIVFNSVIRQQFTIDNMIQKGMKAIGRVLSPIFCDLHSFRNGSLDSLEAPCGTPGRGCFNWTRPGEDYFFDRLDEGFIVGTIFSYLGGYRDGDHICHQMAFALDDETVLDMVAGKLRDSVYRLFDDVPFCSDTIGLIEKLIGVDEHAAQMIGFNSTDAFCGEISKIYHQGGNYNFSFVPLLDLFTNHSHGLEHFVTSESIMHVTTEMMKFAAMIMHSDNIGDAISKSCYMWQDSGPGITMPLFEVDDLCQVVLALNPAELQDVCIHDIIPWIEDLFDHHHDDEERGEENEIDFDFTGILETVGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFYDLPASHLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALEDDAQLDMVANKMKDSIFRFFNDPSFCPVTMDLINRLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSLEPLYVLFANVEEEGEFDPAAIINATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEVDDLCKTVLAFNSAELQDVCIHELFPWIEDVFDHHEDDEMREEENEIDFDFTGILETVGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFHDHPDTVLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALDDDEQLDMVANKMKDSIFRFFNDPSFCPATMDIINKLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSLEPLYVLFADVEEEGNFDPAAIMNATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEVDDLCQTVLAFNSAELQDVCIHELFPWIEDVFDHHDDDEMREEENEIDFDFTGILETVGEEFGFDLHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFHDHPDTVLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALEDDAQLDMVANKMKDSIFRFFNDPSFCPVTMDIINRLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSFEPLYVLFANVEEEGEFDPAAIINATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEIDDLCQLVLEDNSDAVYQVCMGELFPLIESILEDEMYDFNDNHTNTVVDLSNIEICLDNGEVTFEALENLDILAYLLQTTHKLYNLTVFDESNICRAVTSVVESRNDIPTLISEFAVEMMSLFMPLGAEICSCWDQVFDDLFSDSDDSEFGVNQFNEIVRIAVNSIGLDSRHELCDHLIHLKPDNMSDYAQTVIEEILGFTADPEKCACTANRVIDFILPLVNVSMEEINNYLYQYLGYHSVDHVCSFVAQSFSGVIVDPSSINCNSQQEPTTKATTTTQSSLDNNGNSTQSSIDNTGNSTQSPIDNTGDSNETSTDDETCLFITDCAGVCNGNASLDCARECGGSAALDCAFKCGGSATTNECGWCVGGATERPSTLGFDQCGSCIGEGTAVIDCNGDCDGTAYRDACQVCVGGNTSVAEDVSDRFLDCLGICNGSFVTNDCDECVLPFSDGTIFSTADCNGDCNGFADINECSYCVGGNTGNSVDTGLSACGVCNTTIEPDFCLGCDGVADSGLVTDACDVCDGDGSTCFTVDNIAPSIIPANTDYQVKLRGAGFQDATTKQCKFLDSSGEQVSVVDISDEVDDEFNCTANLPQGTYSVYLSTESTELSEAFVTLYVYSEVIINSLSQSEFDIDDSVISISVNMVSTSGAFTAYKDLTTPKAIVFTETERLYYEGTFGGDNDELLEFTGPMLTTSAQVVVYPSFNGIDYLVTPDDAGFTITYYAPAPVAQSLKFSPTGHLLELEFDVAINEDSVTSCDAVFMNITSLGGAAAQCFAFPYLKTIYILPFSSASVNLIGPDDELVLRADVLKKRDEEYARYASGTLTASSPDEAVTVYAALTGTEAISSCGNVRLSALQSFGNAGRFFTYQWSVTSGGSIPSGLTDDLTAAAESAYVTLDGSYLDADVPYTFSMTAENFLGGSDTKTLEVTRSALNKPDVVIYPERVDPNSALISQAFYLRAAVRFYEACGGAEQMEYAWSVDDDSIALNLQTINKPLLYVAAGSLPGDSQVTFTLEVNRESDPSTVTTQTVTITTVYTDLDALIDGGSERTIGVDSESFTLDGSSSFDPDSENEEMTYLWTCQKSNTETGSYEPCVSSETREVFPAEADSKTSTLTLASNNLYSDSTYLFTLEINKLSRTASTSVTILSKSGNPPTISLDESLPEKIKSSSSHTLRVLISHTSDITIDWATVDIDLGYGYVDFSQLNSQSFLIQGDTSPFITYAFVTIPTGTLEEDVAYSFSVTVTDTEGQVSASKLTTNTYGGVSSCTFSLGDGSTSYQELDEITLLTQNCVTDEEAYPLSYQVFREKVESSVTRYNAMTGALAEASSTILGLPSFSDNQNTFVVKVCNDFGSCSSYSLTLDVTPIASFSQEQFDESITNLVEPQKLSGNFLDALVNFNTITSVGVDSTSRKRRAVSETSSTATEQLDLLQNAISSTVLDTATAQTLLDQSDGVVIADLTLSDMDIYLDILIELVAVFSDEGLPESSAEIVLTKTSEIAAELDPQYNSDMLAKITTLRNTLIQGQLSQIPLGGDPTETRSGSVTTRAFYAIPSDTLSTASSNTSFNVKFGSEIESLYGASWSCASGSTCSGVQIMFTQYDDEVDYYSTTDEDKANRASSILEIDLLDPSDNSELSITGLSSPISINMTASRLQSDKVYDCYWWDTSSSSWSQDGLTTVYHSSVLSECQTTHLSAFTLIAVDSPTTIMMATTIVDDASTSTDAVASTATTEISESGSSGLSSTVIIVIAVVISVVLILIIIIAIVVVMSKKSSKVGNADIENSNGNGARNGTSTADGLAPVESANQAFNAPPTAPAHPTHTVQVVAAPASAPASQEPIMDVADLPGSPSPPPDGPHIDTIIKDVQNDSRAQTPMDRAMTPVE

Target ORF information:

RefSeq Version	XM_030976261.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030976261.1

ORF Insert Sequence:

ATGAAGGCAC TTGTGGTGGT TGTAATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCCTC 
GTCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATAG GGCCAAAGCT TAGCTGGCTT TCCGATGGAG CAATGCAAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAAT 
TCTTGGTCCT TTCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCACTCGAAG ATTTGGATTT TAACTGGACC AACCCTTTAT TCGATGATAC TGCGAACATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGTA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGAGTGG TTCTAAACGT CATTGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG GTGCAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGGAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATTTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAC TGGATTCTCT CTATTGGAGA 
GGCAATATGA GCGAGATCGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGAGTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GACTGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AATTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGGTTGACGA TTTATGTAAA 
ACAGTATTGG CCTTTAATTC TGCAGAACTC CAAGATGTCT GCATCCACGA ACTATTTCCG 
TGGATTGAGG ATGTTTTTGA CCACCATGAG GATGATGAAA TGAGGGAAGA AGAAAACGAA 
ATTGACTTTG ATTTCACAGG GATTCTCGAA ACGGTAGGCG AGGAATTCGG ATTCGACTTT 
CACGATTCTC ACCAAAGTTG CTCTCATCTT ACTGGTATTC TCTTTGATGA TACCGTCAGC 
CAAAACTTTC CAATCGACAG CGTTGTGGTA AGGGGAATAA GAGCTGTTGG TAAGATAGTA 
TCACCCATAT TCTGTGATTT TGAATCATTT CATGACCACC CAGATACTGT CTTGGAAGCT 
CCGTGTGCAA CTCCTGGACA AAACTGCTTT GATTGGTCAA GCCGAGGAGA AGACTTCTTC 
TATGAGCGAG AAGACATTGG CTTTGTCATT GGTACAATTT TCAGTTATAT CGGAGGCTAC 
AGAGATGGGG ACCACCTTTG TCACCAAATG GCCTTTGCAC TGGATGATGA TGAACAACTT 
GACATGGTAG CAAATAAAAT GAAGGATAGT ATCTTCCGAT TCTTCAACGA CCCATCATTC 
TGTCCTGCGA CGATGGACAT TATCAACAAG CTCATCGGAT TTGACGAGGA TGCTGCAAGT 
TTACTCGGCT TTGAGAATGC GGATGCCTTC TGCGGGAAGG TCAATCAAAT ATACAATGAA 
GATGGCGATT ATAACGTCAG TTTGGAACCT CTCTATGTTT TGTTTGCCGA TGTTGAAGAA 
GAAGGAAACT TTGACCCTGC AGCCATCATG AATGCCACAA CCGAGATGAT TAAATTCGCC 
GCCATGATCA TGCGTTCAGA CCACATTGGA GATGCCATTT CTAAAACATG CTACGTGTGG 
CAGGAATCTG CTGCAATGTT AAATATTTCA GAGGTTGACG ATTTATGTCA AACAGTATTG 
GCCTTTAATT CTGCAGAACT CCAAGATGTC TGCATCCACG AACTATTTCC CTGGATTGAG 
GACGTTTTTG ACCACCATGA TGATGATGAA ATGAGGGAAG AAGAAAACGA AATTGACTTT 
GATTTCACAG GGATTCTCGA AACGGTAGGC GAGGAATTCG GATTCGACTT ACACGATTCT 
CACCAAAGTT GCTCTCATCT TACTGGTATT CTCTTTGATG ATACCGTCAG CCAAAACTTT 
CCAATCGACA GCGTTGTAGT AAGGGGAATA AGAGCTGTTG GTAAGATAGT ATCACCCATA 
TTCTGTGATT TTGAATCATT TCATGACCAC CCAGATACTG TCTTGGAAGC TCCGTGTGCA 
ACTCCTGGAC AAAACTGCTT TGATTGGTCA AGCCGAGGAG AAGACTTCTT CTATGAGCGG 
GAAGACATTG GCTTTGTCAT TGGTACAATT TTCAGTTATA TTGGGGGCTA CAGAGATGGG 
GACCACCTTT GTCACCAAAT GGCCTTTGCA CTGGAGGATG ATGCACAACT TGACATGGTC 
GCAAATAAAA TGAAGGATAG TATCTTTCGA TTCTTCAACG ACCCGTCATT CTGTCCTGTG 
ACGATGGACA TTATTAACAG GCTCATCGGA TTTGACGAGG ATGCTGCAAG TTTACTCGGC 
TTTGAGAATG CGGATGCCTT CTGCGGAAAG GTCAATCAAA TATACAATGA AGATGGCGAT 
TATAACGTCA GTTTTGAGCC TCTCTATGTT TTGTTTGCCA ATGTTGAAGA AGAAGGAGAA 
TTTGACCCTG CAGCCATTAT AAATGCCACA ACCGAGATGA TTAAATTCGC CGCCATGATC 
ATGCGTTCAG ACCACATTGG AGATGCCATT TCTAAAACAT GCTACGTGTG GCAGGAATCT 
GCTGCAATGT TAAATATTTC AGAGATTGAT GATTTATGTC AATTGGTTCT TGAGGACAAT 
TCCGATGCTG TTTACCAAGT CTGCATGGGT GAACTTTTCC CACTTATTGA GTCAATTCTA 
GAAGACGAGA TGTATGATTT CAATGATAAT CATACAAACA CCGTTGTAGA TTTATCGAAC 
ATTGAGATCT GTCTCGATAA TGGCGAGGTG ACTTTTGAGG CACTTGAAAA TTTAGATATA 
TTGGCATATC TACTACAAAC CACACACAAG CTCTATAATT TAACCGTGTT CGACGAGAGC 
AACATTTGTA GAGCAGTGAC CAGCGTAGTA GAATCTAGAA ATGACATTCC GACACTGATA 
TCTGAATTTG CTGTGGAAAT GATGTCCCTC TTCATGCCGC TTGGTGCAGA AATATGCTCT 
TGTTGGGATC AAGTTTTTGA TGATCTCTTC TCGGACTCCG ACGACTCGGA ATTTGGCGTA 
AACCAATTCA ATGAGATAGT CCGGATTGCT GTGAACAGTA TTGGATTAGA CAGTCGTCAT 
GAACTCTGTG ACCATCTCAT TCATCTAAAG CCGGACAACA TGTCTGATTA CGCACAAACG 
GTGATTGAGG AAATCCTTGG TTTTACGGCT GATCCTGAGA AGTGTGCCTG TACCGCCAAT 
CGTGTCATCG ATTTCATCCT TCCCTTGGTT AATGTTTCAA TGGAGGAAAT CAATAACTAC 
CTTTACCAGT ACCTGGGATA CCATTCTGTG GATCACGTGT GTAGTTTCGT GGCCCAATCC 
TTCTCGGGAG TAATAGTCGA CCCTTCAAGC ATAAACTGTA ACAGCCAACA AGAACCAACA 
ACCAAAGCTA CGACCACAAC TCAATCATCA TTAGACAATA ATGGAAACTC AACTCAATCA 
TCAATAGACA ATACTGGAAA CTCAACTCAA TCACCAATAG ACAATACTGG AGACTCGAAT 
GAAACTTCGA CTGACGACGA AACCTGTCTC TTCATCACTG ACTGCGCCGG TGTATGCAAC 
GGTAACGCTA GTTTGGATTG CGCCCGAGAA TGCGGCGGCA GCGCGGCACT CGACTGCGCC 
TTCAAGTGCG GTGGCTCTGC GACCACGAAC GAATGTGGAT GGTGCGTCGG TGGAGCTACG 
GAAAGACCAA GCACTTTAGG TTTCGACCAG TGTGGTTCCT GCATTGGGGA AGGCACTGCG 
GTCATCGACT GCAATGGTGA CTGTGACGGT ACTGCTTACC GCGATGCCTG TCAAGTCTGC 
GTAGGTGGCA ATACGAGTGT GGCTGAGGAT GTAAGCGATC GTTTCTTGGA CTGCCTAGGT 
ATCTGTAATG GTAGTTTTGT GACCAATGAT TGTGATGAAT GCGTGCTGCC ATTCAGCGAT 
GGGACTATTT TCTCCACGGC GGATTGTAAC GGTGACTGCA ATGGATTTGC CGACATAAAT 
GAGTGTAGTT ACTGTGTTGG GGGTAATACC GGTAACTCTG TGGATACTGG ATTGTCTGCA 
TGTGGAGTCT GCAATACTAC GATCGAACCA GACTTCTGCC TCGGCTGTGA TGGCGTCGCT 
GATAGTGGCC TAGTGACCGA TGCGTGTGAT GTATGCGATG GGGATGGTAG CACTTGTTTC 
ACCGTGGACA ATATAGCTCC CTCTATCATC CCAGCTAACA CCGACTATCA GGTTAAACTT 
CGTGGTGCCG GATTCCAAGA TGCGACTACC AAACAGTGTA AGTTCCTGGA TAGCAGCGGT 
GAGCAGGTCA GCGTGGTCGA TATCAGCGAT GAGGTGGATG ACGAGTTCAA CTGCACCGCT 
AATCTTCCTC AAGGAACGTA TTCTGTTTAC CTTTCGACGG AGTCAACGGA ACTATCAGAA 
GCATTCGTCA CCCTCTATGT CTATTCAGAA GTTATAATAA ACAGCTTATC CCAGAGCGAG 
TTTGATATAG ATGATAGTGT GATTTCCATC TCTGTCAACA TGGTATCAAC AAGCGGAGCC 
TTTACGGCAT ACAAGGACTT GACCACTCCA AAAGCTATCG TCTTCACAGA GACCGAGAGA 
CTTTACTACG AAGGAACTTT TGGAGGAGAC AACGACGAGC TTCTCGAGTT TACGGGTCCG 
ATGCTGACTA CCTCGGCTCA AGTTGTCGTT TACCCGTCCT TCAACGGAAT CGACTACCTG 
GTCACCCCCG ATGATGCTGG TTTCACGATC ACCTACTACG CCCCTGCACC GGTCGCTCAG 
AGTCTTAAAT TCTCCCCTAC TGGACATCTT CTGGAACTCG AGTTCGACGT CGCAATCAAC 
GAAGACTCGG TGACATCATG TGATGCAGTA TTCATGAATA TCACCAGCCT GGGTGGTGCT 
GCTGCCCAGT GCTTTGCATT CCCTTACCTA AAAACTATCT ATATACTACC CTTCAGTTCG 
GCCAGTGTAA ATCTTATTGG ACCAGATGAT GAGCTAGTGC TGCGCGCTGA CGTTCTGAAA 
AAACGTGATG AGGAATACGC TCGGTACGCC AGCGGTACTC TTACCGCATC CTCCCCCGAC 
GAGGCGGTAA CCGTCTACGC GGCTCTGACC GGTACAGAAG CAATTAGCAG CTGCGGTAAT 
GTACGACTTA GTGCTCTGCA ATCCTTTGGA AACGCAGGAC GTTTTTTCAC CTATCAGTGG 
TCTGTGACCA GTGGCGGTTC GATCCCATCT GGCTTGACGG ATGATCTAAC AGCCGCAGCG 
GAGTCCGCCT ATGTCACTCT CGATGGATCC TACCTCGACG CGGACGTGCC GTATACATTC 
AGCATGACAG CAGAGAACTT CCTCGGAGGC AGTGACACAA AGACCTTGGA GGTGACTCGG 
TCTGCTCTCA ACAAACCAGA CGTCGTGATC TACCCGGAAA GAGTTGATCC TAATAGTGCC 
CTTATCTCGC AAGCGTTCTA CCTCCGCGCT GCTGTACGAT TCTACGAGGC CTGCGGTGGA 
GCTGAGCAGA TGGAGTATGC CTGGTCTGTA GATGATGATA GTATTGCTCT TAACCTACAA 
ACCATAAACA AACCACTCCT GTATGTGGCA GCTGGTTCTT TACCAGGCGA CTCCCAAGTG 
ACCTTCACAC TGGAGGTCAA CCGGGAAAGT GATCCCAGCA CGGTGACCAC GCAGACCGTG 
ACCATTACCA CGGTCTACAC CGACCTCGAC GCCCTAATCG ATGGCGGGAG TGAGCGGACC 
ATCGGCGTCG ACAGTGAGTC GTTCACACTG GATGGATCGT CGTCGTTTGA TCCCGACAGT 
GAAAATGAAG AGATGACCTA CCTCTGGACT TGTCAGAAGT CGAACACTGA GACAGGCAGC 
TACGAGCCTT GTGTATCAAG CGAGACACGT GAGGTGTTCC CGGCGGAAGC TGACTCCAAA 
ACAAGTACTC TCACGTTAGC TTCCAACAAT CTTTACTCGG ACTCCACCTA TCTGTTCACT 
CTGGAGATCA ACAAACTGTC AAGGACAGCA TCGACCAGTG TCACCATCCT TTCCAAGTCT 
GGCAATCCTC CAACGATCAG TCTCGATGAA TCCCTTCCTG AGAAGATAAA GAGCAGTAGT 
TCTCATACGC TACGAGTATT GATCAGCCAT ACATCAGATA TTACCATTGA CTGGGCTACT 
GTCGACATAG ATCTTGGATA TGGATACGTT GACTTTTCAC AGCTGAATAG TCAGTCATTC 
CTTATACAAG GAGATACATC ACCCTTCATC ACATACGCCT TTGTAACTAT CCCCACAGGA 
ACTTTGGAAG AGGATGTTGC CTACTCTTTC TCAGTGACGG TAACTGATAC CGAAGGACAG 
GTGTCAGCTA GTAAATTGAC TACCAACACG TATGGAGGAG TATCATCCTG TACATTCAGT 
TTGGGTGATG GTTCAACCAG CTATCAAGAA CTTGACGAGA TCACTCTCCT AACTCAGAAC 
TGCGTGACTG ATGAAGAAGC TTACCCTCTG TCTTACCAAG TGTTCCGTGA GAAGGTCGAA 
TCATCTGTCA CCCGTTACAA CGCGATGACG GGTGCATTGG CAGAAGCGAG CAGCACTATA 
TTGGGTCTGC CGTCCTTTAG CGACAACCAG AACACATTTG TTGTCAAGGT GTGCAATGAT 
TTTGGAAGCT GTTCCTCGTA CAGTCTTACA TTGGACGTCA CTCCCATCGC ATCTTTCAGT 
CAAGAACAGT TTGATGAAAG TATAACCAAT CTGGTAGAAC CACAAAAGCT CTCAGGGAAT 
TTCCTAGATG CGCTGGTAAA CTTCAACACC ATCACGTCAG TGGGTGTCGA CAGCACGAGT 
AGGAAACGGA GAGCAGTCTC GGAGACATCC AGCACAGCTA CTGAGCAGCT GGATTTACTT 
CAGAATGCCA TCTCGTCGAC GGTCCTTGAC ACGGCCACCG CCCAGACTCT GCTTGATCAG 
TCGGATGGCG TGGTCATTGC TGATCTCACA CTGTCTGATA TGGACATATA CCTGGATATT 
TTAATTGAGC TCGTAGCTGT TTTCAGTGAT GAAGGGCTTC CAGAGTCTAG CGCCGAGATC 
GTTCTTACAA AGACAAGCGA GATCGCTGCT GAACTCGACC CTCAATACAA CTCGGACATG 
CTTGCCAAAA TTACGACCCT GAGAAACACA TTAATCCAGG GCCAGCTGAG TCAGATCCCA 
CTTGGAGGGG ACCCTACGGA GACCAGGTCT GGTTCCGTTA CCACCAGGGC CTTCTATGCT 
ATCCCTAGTG ATACCCTCAG TACTGCATCC AGCAACACCT CCTTCAACGT CAAGTTCGGA 
AGCGAGATCG AAAGCTTATA TGGTGCAAGT TGGTCGTGTG CTTCTGGAAG CACTTGTTCT 
GGTGTTCAGA TCATGTTTAC GCAGTACGAC GACGAGGTTG ATTACTACTC GACTACGGAC 
GAGGATAAGG CGAACAGAGC ATCTTCTATC CTTGAAATCG ATCTGCTTGA CCCGAGTGAC 
AATTCTGAGT TGAGTATTAC TGGACTGAGC AGCCCCATAT CAATCAACAT GACCGCTTCA 
CGCCTCCAAT CCGACAAAGT CTATGACTGC TATTGGTGGG ACACTAGCAG CAGCTCTTGG 
AGCCAGGATG GACTTACGAC CGTCTACCAC TCCTCGGTCT TGTCTGAGTG CCAAACTACT 
CATCTTTCAG CCTTCACCCT GATCGCTGTT GATTCTCCTA CTACGATTAT GATGGCTACC 
ACGATAGTTG ATGACGCCAG TACTTCTACC GATGCAGTAG CCTCGACTGC GACAACGGAA 
ATCAGTGAGA GCGGCAGCAG TGGTCTAAGT TCTACCGTCA TCATCGTCAT TGCCGTGGTC 
ATATCTGTCG TTTTGATCCT AATTATTATC ATCGCCATCG TGGTCGTGAT GTCGAAGAAG 
TCCTCCAAGG TCGGTAATGC TGATATCGAG AACTCGAACG GCAATGGTGC CAGGAATGGG 
ACCAGCACCG CCGATGGACT CGCTCCTGTT GAATCTGCGA ACCAGGCATT CAATGCCCCA 
CCAACAGCAC CGGCCCACCC AACCCATACT GTTCAAGTGG TCGCTGCCCC AGCTTCGGCA 
CCTGCTTCTC AAGAGCCTAT CATGGACGTT GCAGACCTTC CTGGATCCCC ATCACCCCCT 
CCTGATGGAC CCCATATTGA TACCATCATA AAGGATGTTC AGAATGATAG TAGGGCCCAA 
ACTCCAATGG ATAGAGCCAT GACGCCCGTT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REJ8 ( XM_030976262.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030832122.1 Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X2, mRNA.

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CloneID	OSd71217
Clone ID Related Accession (Same CDS sequence)	XM_030976261.1 , XM_030976262.1
Accession Version	XM_030976262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	receptor for egg jelly 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145615.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC TTGTGGTGGT TGTAATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCCTC 
GTCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATAG GGCCAAAGCT TAGCTGGCTT TCCGATGGAG CAATGCAAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAAT 
TCTTGGTCCT TTCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCACTCGAAG ATTTGGATTT TAACTGGACC AACCCTTTAT TCGATGATAC TGCGAACATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGTA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGAGTGG TTCTAAACGT CATTGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG GTGCAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGGAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATTTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAC TGGATTCTCT CTATTGGAGA 
GGCAATATGA GCGAGATCGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGAGTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GACTGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AATTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGGTTGACGA TTTATGTAAA 
ACAGTATTGG CCTTTAATTC TGCAGAACTC CAAGATGTCT GCATCCACGA ACTATTTCCG 
TGGATTGAGG ATGTTTTTGA CCACCATGAG GATGATGAAA TGAGGGAAGA AGAAAACGAA 
ATTGACTTTG ATTTCACAGG GATTCTCGAA ACGGTAGGCG AGGAATTCGG ATTCGACTTT 
CACGATTCTC ACCAAAGTTG CTCTCATCTT ACTGGTATTC TCTTTGATGA TACCGTCAGC 
CAAAACTTTC CAATCGACAG CGTTGTGGTA AGGGGAATAA GAGCTGTTGG TAAGATAGTA 
TCACCCATAT TCTGTGATTT TGAATCATTT CATGACCACC CAGATACTGT CTTGGAAGCT 
CCGTGTGCAA CTCCTGGACA AAACTGCTTT GATTGGTCAA GCCGAGGAGA AGACTTCTTC 
TATGAGCGAG AAGACATTGG CTTTGTCATT GGTACAATTT TCAGTTATAT CGGAGGCTAC 
AGAGATGGGG ACCACCTTTG TCACCAAATG GCCTTTGCAC TGGATGATGA TGAACAACTT 
GACATGGTAG CAAATAAAAT GAAGGATAGT ATCTTCCGAT TCTTCAACGA CCCATCATTC 
TGTCCTGCGA CGATGGACAT TATCAACAAG CTCATCGGAT TTGACGAGGA TGCTGCAAGT 
TTACTCGGCT TTGAGAATGC GGATGCCTTC TGCGGGAAGG TCAATCAAAT ATACAATGAA 
GATGGCGATT ATAACGTCAG TTTGGAACCT CTCTATGTTT TGTTTGCCGA TGTTGAAGAA 
GAAGGAAACT TTGACCCTGC AGCCATCATG AATGCCACAA CCGAGATGAT TAAATTCGCC 
GCCATGATCA TGCGTTCAGA CCACATTGGA GATGCCATTT CTAAAACATG CTACGTGTGG 
CAGGAATCTG CTGCAATGTT AAATATTTCA GAGGTTGACG ATTTATGTCA AACAGTATTG 
GCCTTTAATT CTGCAGAACT CCAAGATGTC TGCATCCACG AACTATTTCC CTGGATTGAG 
GACGTTTTTG ACCACCATGA TGATGATGAA ATGAGGGAAG AAGAAAACGA AATTGACTTT 
GATTTCACAG GGATTCTCGA AACGGTAGGC GAGGAATTCG GATTCGACTT ACACGATTCT 
CACCAAAGTT GCTCTCATCT TACTGGTATT CTCTTTGATG ATACCGTCAG CCAAAACTTT 
CCAATCGACA GCGTTGTAGT AAGGGGAATA AGAGCTGTTG GTAAGATAGT ATCACCCATA 
TTCTGTGATT TTGAATCATT TCATGACCAC CCAGATACTG TCTTGGAAGC TCCGTGTGCA 
ACTCCTGGAC AAAACTGCTT TGATTGGTCA AGCCGAGGAG AAGACTTCTT CTATGAGCGG 
GAAGACATTG GCTTTGTCAT TGGTACAATT TTCAGTTATA TTGGGGGCTA CAGAGATGGG 
GACCACCTTT GTCACCAAAT GGCCTTTGCA CTGGAGGATG ATGCACAACT TGACATGGTC 
GCAAATAAAA TGAAGGATAG TATCTTTCGA TTCTTCAACG ACCCGTCATT CTGTCCTGTG 
ACGATGGACA TTATTAACAG GCTCATCGGA TTTGACGAGG ATGCTGCAAG TTTACTCGGC 
TTTGAGAATG CGGATGCCTT CTGCGGAAAG GTCAATCAAA TATACAATGA AGATGGCGAT 
TATAACGTCA GTTTTGAGCC TCTCTATGTT TTGTTTGCCA ATGTTGAAGA AGAAGGAGAA 
TTTGACCCTG CAGCCATTAT AAATGCCACA ACCGAGATGA TTAAATTCGC CGCCATGATC 
ATGCGTTCAG ACCACATTGG AGATGCCATT TCTAAAACAT GCTACGTGTG GCAGGAATCT 
GCTGCAATGT TAAATATTTC AGAGATTGAT GATTTATGTC AATTGGTTCT TGAGGACAAT 
TCCGATGCTG TTTACCAAGT CTGCATGGGT GAACTTTTCC CACTTATTGA GTCAATTCTA 
GAAGACGAGA TGTATGATTT CAATGATAAT CATACAAACA CCGTTGTAGA TTTATCGAAC 
ATTGAGATCT GTCTCGATAA TGGCGAGGTG ACTTTTGAGG CACTTGAAAA TTTAGATATA 
TTGGCATATC TACTACAAAC CACACACAAG CTCTATAATT TAACCGTGTT CGACGAGAGC 
AACATTTGTA GAGCAGTGAC CAGCGTAGTA GAATCTAGAA ATGACATTCC GACACTGATA 
TCTGAATTTG CTGTGGAAAT GATGTCCCTC TTCATGCCGC TTGGTGCAGA AATATGCTCT 
TGTTGGGATC AAGTTTTTGA TGATCTCTTC TCGGACTCCG ACGACTCGGA ATTTGGCGTA 
AACCAATTCA ATGAGATAGT CCGGATTGCT GTGAACAGTA TTGGATTAGA CAGTCGTCAT 
GAACTCTGTG ACCATCTCAT TCATCTAAAG CCGGACAACA TGTCTGATTA CGCACAAACG 
GTGATTGAGG AAATCCTTGG TTTTACGGCT GATCCTGAGA AGTGTGCCTG TACCGCCAAT 
CGTGTCATCG ATTTCATCCT TCCCTTGGTT AATGTTTCAA TGGAGGAAAT CAATAACTAC 
CTTTACCAGT ACCTGGGATA CCATTCTGTG GATCACGTGT GTAGTTTCGT GGCCCAATCC 
TTCTCGGGAG TAATAGTCGA CCCTTCAAGC ATAAACTGTA ACAGCCAACA AGAACCAACA 
ACCAAAGCTA CGACCACAAC TCAATCATCA TTAGACAATA ATGGAAACTC AACTCAATCA 
TCAATAGACA ATACTGGAAA CTCAACTCAA TCACCAATAG ACAATACTGG AGACTCGAAT 
GAAACTTCGA CTGACGACGA AACCTGTCTC TTCATCACTG ACTGCGCCGG TGTATGCAAC 
GGTAACGCTA GTTTGGATTG CGCCCGAGAA TGCGGCGGCA GCGCGGCACT CGACTGCGCC 
TTCAAGTGCG GTGGCTCTGC GACCACGAAC GAATGTGGAT GGTGCGTCGG TGGAGCTACG 
GAAAGACCAA GCACTTTAGG TTTCGACCAG TGTGGTTCCT GCATTGGGGA AGGCACTGCG 
GTCATCGACT GCAATGGTGA CTGTGACGGT ACTGCTTACC GCGATGCCTG TCAAGTCTGC 
GTAGGTGGCA ATACGAGTGT GGCTGAGGAT GTAAGCGATC GTTTCTTGGA CTGCCTAGGT 
ATCTGTAATG GTAGTTTTGT GACCAATGAT TGTGATGAAT GCGTGCTGCC ATTCAGCGAT 
GGGACTATTT TCTCCACGGC GGATTGTAAC GGTGACTGCA ATGGATTTGC CGACATAAAT 
GAGTGTAGTT ACTGTGTTGG GGGTAATACC GGTAACTCTG TGGATACTGG ATTGTCTGCA 
TGTGGAGTCT GCAATACTAC GATCGAACCA GACTTCTGCC TCGGCTGTGA TGGCGTCGCT 
GATAGTGGCC TAGTGACCGA TGCGTGTGAT GTATGCGATG GGGATGGTAG CACTTGTTTC 
ACCGTGGACA ATATAGCTCC CTCTATCATC CCAGCTAACA CCGACTATCA GGTTAAACTT 
CGTGGTGCCG GATTCCAAGA TGCGACTACC AAACAGTGTA AGTTCCTGGA TAGCAGCGGT 
GAGCAGGTCA GCGTGGTCGA TATCAGCGAT GAGGTGGATG ACGAGTTCAA CTGCACCGCT 
AATCTTCCTC AAGGAACGTA TTCTGTTTAC CTTTCGACGG AGTCAACGGA ACTATCAGAA 
GCATTCGTCA CCCTCTATGT CTATTCAGAA GTTATAATAA ACAGCTTATC CCAGAGCGAG 
TTTGATATAG ATGATAGTGT GATTTCCATC TCTGTCAACA TGGTATCAAC AAGCGGAGCC 
TTTACGGCAT ACAAGGACTT GACCACTCCA AAAGCTATCG TCTTCACAGA GACCGAGAGA 
CTTTACTACG AAGGAACTTT TGGAGGAGAC AACGACGAGC TTCTCGAGTT TACGGGTCCG 
ATGCTGACTA CCTCGGCTCA AGTTGTCGTT TACCCGTCCT TCAACGGAAT CGACTACCTG 
GTCACCCCCG ATGATGCTGG TTTCACGATC ACCTACTACG CCCCTGCACC GGTCGCTCAG 
AGTCTTAAAT TCTCCCCTAC TGGACATCTT CTGGAACTCG AGTTCGACGT CGCAATCAAC 
GAAGACTCGG TGACATCATG TGATGCAGTA TTCATGAATA TCACCAGCCT GGGTGGTGCT 
GCTGCCCAGT GCTTTGCATT CCCTTACCTA AAAACTATCT ATATACTACC CTTCAGTTCG 
GCCAGTGTAA ATCTTATTGG ACCAGATGAT GAGCTAGTGC TGCGCGCTGA CGTTCTGAAA 
AAACGTGATG AGGAATACGC TCGGTACGCC AGCGGTACTC TTACCGCATC CTCCCCCGAC 
GAGGCGGTAA CCGTCTACGC GGCTCTGACC GGTACAGAAG CAATTAGCAG CTGCGGTAAT 
GTACGACTTA GTGCTCTGCA ATCCTTTGGA AACGCAGGAC GTTTTTTCAC CTATCAGTGG 
TCTGTGACCA GTGGCGGTTC GATCCCATCT GGCTTGACGG ATGATCTAAC AGCCGCAGCG 
GAGTCCGCCT ATGTCACTCT CGATGGATCC TACCTCGACG CGGACGTGCC GTATACATTC 
AGCATGACAG CAGAGAACTT CCTCGGAGGC AGTGACACAA AGACCTTGGA GGTGACTCGG 
TCTGCTCTCA ACAAACCAGA CGTCGTGATC TACCCGGAAA GAGTTGATCC TAATAGTGCC 
CTTATCTCGC AAGCGTTCTA CCTCCGCGCT GCTGTACGAT TCTACGAGGC CTGCGGTGGA 
GCTGAGCAGA TGGAGTATGC CTGGTCTGTA GATGATGATA GTATTGCTCT TAACCTACAA 
ACCATAAACA AACCACTCCT GTATGTGGCA GCTGGTTCTT TACCAGGCGA CTCCCAAGTG 
ACCTTCACAC TGGAGGTCAA CCGGGAAAGT GATCCCAGCA CGGTGACCAC GCAGACCGTG 
ACCATTACCA CGGTCTACAC CGACCTCGAC GCCCTAATCG ATGGCGGGAG TGAGCGGACC 
ATCGGCGTCG ACAGTGAGTC GTTCACACTG GATGGATCGT CGTCGTTTGA TCCCGACAGT 
GAAAATGAAG AGATGACCTA CCTCTGGACT TGTCAGAAGT CGAACACTGA GACAGGCAGC 
TACGAGCCTT GTGTATCAAG CGAGACACGT GAGGTGTTCC CGGCGGAAGC TGACTCCAAA 
ACAAGTACTC TCACGTTAGC TTCCAACAAT CTTTACTCGG ACTCCACCTA TCTGTTCACT 
CTGGAGATCA ACAAACTGTC AAGGACAGCA TCGACCAGTG TCACCATCCT TTCCAAGTCT 
GGCAATCCTC CAACGATCAG TCTCGATGAA TCCCTTCCTG AGAAGATAAA GAGCAGTAGT 
TCTCATACGC TACGAGTATT GATCAGCCAT ACATCAGATA TTACCATTGA CTGGGCTACT 
GTCGACATAG ATCTTGGATA TGGATACGTT GACTTTTCAC AGCTGAATAG TCAGTCATTC 
CTTATACAAG GAGATACATC ACCCTTCATC ACATACGCCT TTGTAACTAT CCCCACAGGA 
ACTTTGGAAG AGGATGTTGC CTACTCTTTC TCAGTGACGG TAACTGATAC CGAAGGACAG 
GTGTCAGCTA GTAAATTGAC TACCAACACG TATGGAGGAG TATCATCCTG TACATTCAGT 
TTGGGTGATG GTTCAACCAG CTATCAAGAA CTTGACGAGA TCACTCTCCT AACTCAGAAC 
TGCGTGACTG ATGAAGAAGC TTACCCTCTG TCTTACCAAG TGTTCCGTGA GAAGGTCGAA 
TCATCTGTCA CCCGTTACAA CGCGATGACG GGTGCATTGG CAGAAGCGAG CAGCACTATA 
TTGGGTCTGC CGTCCTTTAG CGACAACCAG AACACATTTG TTGTCAAGGT GTGCAATGAT 
TTTGGAAGCT GTTCCTCGTA CAGTCTTACA TTGGACGTCA CTCCCATCGC ATCTTTCAGT 
CAAGAACAGT TTGATGAAAG TATAACCAAT CTGGTAGAAC CACAAAAGCT CTCAGGGAAT 
TTCCTAGATG CGCTGGTAAA CTTCAACACC ATCACGTCAG TGGGTGTCGA CAGCACGAGT 
AGGAAACGGA GAGCAGTCTC GGAGACATCC AGCACAGCTA CTGAGCAGCT GGATTTACTT 
CAGAATGCCA TCTCGTCGAC GGTCCTTGAC ACGGCCACCG CCCAGACTCT GCTTGATCAG 
TCGGATGGCG TGGTCATTGC TGATCTCACA CTGTCTGATA TGGACATATA CCTGGATATT 
TTAATTGAGC TCGTAGCTGT TTTCAGTGAT GAAGGGCTTC CAGAGTCTAG CGCCGAGATC 
GTTCTTACAA AGACAAGCGA GATCGCTGCT GAACTCGACC CTCAATACAA CTCGGACATG 
CTTGCCAAAA TTACGACCCT GAGAAACACA TTAATCCAGG GCCAGCTGAG TCAGATCCCA 
CTTGGAGGGG ACCCTACGGA GACCAGGTCT GGTTCCGTTA CCACCAGGGC CTTCTATGCT 
ATCCCTAGTG ATACCCTCAG TACTGCATCC AGCAACACCT CCTTCAACGT CAAGTTCGGA 
AGCGAGATCG AAAGCTTATA TGGTGCAAGT TGGTCGTGTG CTTCTGGAAG CACTTGTTCT 
GGTGTTCAGA TCATGTTTAC GCAGTACGAC GACGAGGTTG ATTACTACTC GACTACGGAC 
GAGGATAAGG CGAACAGAGC ATCTTCTATC CTTGAAATCG ATCTGCTTGA CCCGAGTGAC 
AATTCTGAGT TGAGTATTAC TGGACTGAGC AGCCCCATAT CAATCAACAT GACCGCTTCA 
CGCCTCCAAT CCGACAAAGT CTATGACTGC TATTGGTGGG ACACTAGCAG CAGCTCTTGG 
AGCCAGGATG GACTTACGAC CGTCTACCAC TCCTCGGTCT TGTCTGAGTG CCAAACTACT 
CATCTTTCAG CCTTCACCCT GATCGCTGTT GATTCTCCTA CTACGATTAT GATGGCTACC 
ACGATAGTTG ATGACGCCAG TACTTCTACC GATGCAGTAG CCTCGACTGC GACAACGGAA 
ATCAGTGAGA GCGGCAGCAG TGGTCTAAGT TCTACCGTCA TCATCGTCAT TGCCGTGGTC 
ATATCTGTCG TTTTGATCCT AATTATTATC ATCGCCATCG TGGTCGTGAT GTCGAAGAAG 
TCCTCCAAGG TCGGTAATGC TGATATCGAG AACTCGAACG GCAATGGTGC CAGGAATGGG 
ACCAGCACCG CCGATGGACT CGCTCCTGTT GAATCTGCGA ACCAGGCATT CAATGCCCCA 
CCAACAGCAC CGGCCCACCC AACCCATACT GTTCAAGTGG TCGCTGCCCC AGCTTCGGCA 
CCTGCTTCTC AAGAGCCTAT CATGGACGTT GCAGACCTTC CTGGATCCCC ATCACCCCCT 
CCTGATGGAC CCCATATTGA TACCATCATA AAGGATGTTC AGAATGATAG TAGGGCCCAA 
ACTCCAATGG ATAGAGCCAT GACGCCCGTT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030832122.1
CDS	213..10988
Translation	MKALVVVVIFSCLVAGAARAEESTEWQVEWTTEPAYNLSLVEPFTVPGYLLPDLNISDIISTVGMMYQAEDLVHAIGSLCVEIGPKLSWLSDGAMQELCDPVLEALRNGSYAEAYSICQNSWSFLETLIGGEEGGLEGEGEIDHGPIRRKRWGLSVPSIELPPLPSSPIQAVLNITVPFSPLEDLDFNWTNPLFDDTANMVENIGLIPDLNRNWSFPIFDGTGIEVDFPYPEFSDKYGICSSLAGLISYNQPSSPSHGSIFGVVLNVIVAEQVRSIPGKCLSFFDGEEGSGDIDDDPSFKHEPGAMIWAFATLGGFDDIEQICNVSSWALLMRDEGKFELFNELVNQITTSIYSVISSRSRCIEFADSLHANIGLAPGNYSHFTSSEALCDEILDSLYWRGNMSEIDFYNYDSMDEKDAFIYLLSIVEILSHKSDMASLEFLCRMSPYADEMFCTSLAYNDYRDVSREDIIEHCLRYVAFDGFEFDFLALLNAIGAEFEFDFYDFDQTCSHLTDIVFNSVIRQQFTIDNMIQKGMKAIGRVLSPIFCDLHSFRNGSLDSLEAPCGTPGRGCFNWTRPGEDYFFDRLDEGFIVGTIFSYLGGYRDGDHICHQMAFALDDETVLDMVAGKLRDSVYRLFDDVPFCSDTIGLIEKLIGVDEHAAQMIGFNSTDAFCGEISKIYHQGGNYNFSFVPLLDLFTNHSHGLEHFVTSESIMHVTTEMMKFAAMIMHSDNIGDAISKSCYMWQDSGPGITMPLFEVDDLCQVVLALNPAELQDVCIHDIIPWIEDLFDHHHDDEERGEENEIDFDFTGILETVGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFYDLPASHLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALEDDAQLDMVANKMKDSIFRFFNDPSFCPVTMDLINRLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSLEPLYVLFANVEEEGEFDPAAIINATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEVDDLCKTVLAFNSAELQDVCIHELFPWIEDVFDHHEDDEMREEENEIDFDFTGILETVGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFHDHPDTVLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALDDDEQLDMVANKMKDSIFRFFNDPSFCPATMDIINKLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSLEPLYVLFADVEEEGNFDPAAIMNATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEVDDLCQTVLAFNSAELQDVCIHELFPWIEDVFDHHDDDEMREEENEIDFDFTGILETVGEEFGFDLHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFHDHPDTVLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALEDDAQLDMVANKMKDSIFRFFNDPSFCPVTMDIINRLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSFEPLYVLFANVEEEGEFDPAAIINATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEIDDLCQLVLEDNSDAVYQVCMGELFPLIESILEDEMYDFNDNHTNTVVDLSNIEICLDNGEVTFEALENLDILAYLLQTTHKLYNLTVFDESNICRAVTSVVESRNDIPTLISEFAVEMMSLFMPLGAEICSCWDQVFDDLFSDSDDSEFGVNQFNEIVRIAVNSIGLDSRHELCDHLIHLKPDNMSDYAQTVIEEILGFTADPEKCACTANRVIDFILPLVNVSMEEINNYLYQYLGYHSVDHVCSFVAQSFSGVIVDPSSINCNSQQEPTTKATTTTQSSLDNNGNSTQSSIDNTGNSTQSPIDNTGDSNETSTDDETCLFITDCAGVCNGNASLDCARECGGSAALDCAFKCGGSATTNECGWCVGGATERPSTLGFDQCGSCIGEGTAVIDCNGDCDGTAYRDACQVCVGGNTSVAEDVSDRFLDCLGICNGSFVTNDCDECVLPFSDGTIFSTADCNGDCNGFADINECSYCVGGNTGNSVDTGLSACGVCNTTIEPDFCLGCDGVADSGLVTDACDVCDGDGSTCFTVDNIAPSIIPANTDYQVKLRGAGFQDATTKQCKFLDSSGEQVSVVDISDEVDDEFNCTANLPQGTYSVYLSTESTELSEAFVTLYVYSEVIINSLSQSEFDIDDSVISISVNMVSTSGAFTAYKDLTTPKAIVFTETERLYYEGTFGGDNDELLEFTGPMLTTSAQVVVYPSFNGIDYLVTPDDAGFTITYYAPAPVAQSLKFSPTGHLLELEFDVAINEDSVTSCDAVFMNITSLGGAAAQCFAFPYLKTIYILPFSSASVNLIGPDDELVLRADVLKKRDEEYARYASGTLTASSPDEAVTVYAALTGTEAISSCGNVRLSALQSFGNAGRFFTYQWSVTSGGSIPSGLTDDLTAAAESAYVTLDGSYLDADVPYTFSMTAENFLGGSDTKTLEVTRSALNKPDVVIYPERVDPNSALISQAFYLRAAVRFYEACGGAEQMEYAWSVDDDSIALNLQTINKPLLYVAAGSLPGDSQVTFTLEVNRESDPSTVTTQTVTITTVYTDLDALIDGGSERTIGVDSESFTLDGSSSFDPDSENEEMTYLWTCQKSNTETGSYEPCVSSETREVFPAEADSKTSTLTLASNNLYSDSTYLFTLEINKLSRTASTSVTILSKSGNPPTISLDESLPEKIKSSSSHTLRVLISHTSDITIDWATVDIDLGYGYVDFSQLNSQSFLIQGDTSPFITYAFVTIPTGTLEEDVAYSFSVTVTDTEGQVSASKLTTNTYGGVSSCTFSLGDGSTSYQELDEITLLTQNCVTDEEAYPLSYQVFREKVESSVTRYNAMTGALAEASSTILGLPSFSDNQNTFVVKVCNDFGSCSSYSLTLDVTPIASFSQEQFDESITNLVEPQKLSGNFLDALVNFNTITSVGVDSTSRKRRAVSETSSTATEQLDLLQNAISSTVLDTATAQTLLDQSDGVVIADLTLSDMDIYLDILIELVAVFSDEGLPESSAEIVLTKTSEIAAELDPQYNSDMLAKITTLRNTLIQGQLSQIPLGGDPTETRSGSVTTRAFYAIPSDTLSTASSNTSFNVKFGSEIESLYGASWSCASGSTCSGVQIMFTQYDDEVDYYSTTDEDKANRASSILEIDLLDPSDNSELSITGLSSPISINMTASRLQSDKVYDCYWWDTSSSSWSQDGLTTVYHSSVLSECQTTHLSAFTLIAVDSPTTIMMATTIVDDASTSTDAVASTATTEISESGSSGLSSTVIIVIAVVISVVLILIIIIAIVVVMSKKSSKVGNADIENSNGNGARNGTSTADGLAPVESANQAFNAPPTAPAHPTHTVQVVAAPASAPASQEPIMDVADLPGSPSPPPDGPHIDTIIKDVQNDSRAQTPMDRAMTPVE

Target ORF information:

RefSeq Version	XM_030976262.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030976262.1

ORF Insert Sequence:

ATGAAGGCAC TTGTGGTGGT TGTAATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCCTC 
GTCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATAG GGCCAAAGCT TAGCTGGCTT TCCGATGGAG CAATGCAAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAAT 
TCTTGGTCCT TTCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCACTCGAAG ATTTGGATTT TAACTGGACC AACCCTTTAT TCGATGATAC TGCGAACATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGTA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGAGTGG TTCTAAACGT CATTGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG GTGCAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGGAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATTTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAC TGGATTCTCT CTATTGGAGA 
GGCAATATGA GCGAGATCGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGAGTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GACTGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AATTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGGTTGACGA TTTATGTAAA 
ACAGTATTGG CCTTTAATTC TGCAGAACTC CAAGATGTCT GCATCCACGA ACTATTTCCG 
TGGATTGAGG ATGTTTTTGA CCACCATGAG GATGATGAAA TGAGGGAAGA AGAAAACGAA 
ATTGACTTTG ATTTCACAGG GATTCTCGAA ACGGTAGGCG AGGAATTCGG ATTCGACTTT 
CACGATTCTC ACCAAAGTTG CTCTCATCTT ACTGGTATTC TCTTTGATGA TACCGTCAGC 
CAAAACTTTC CAATCGACAG CGTTGTGGTA AGGGGAATAA GAGCTGTTGG TAAGATAGTA 
TCACCCATAT TCTGTGATTT TGAATCATTT CATGACCACC CAGATACTGT CTTGGAAGCT 
CCGTGTGCAA CTCCTGGACA AAACTGCTTT GATTGGTCAA GCCGAGGAGA AGACTTCTTC 
TATGAGCGAG AAGACATTGG CTTTGTCATT GGTACAATTT TCAGTTATAT CGGAGGCTAC 
AGAGATGGGG ACCACCTTTG TCACCAAATG GCCTTTGCAC TGGATGATGA TGAACAACTT 
GACATGGTAG CAAATAAAAT GAAGGATAGT ATCTTCCGAT TCTTCAACGA CCCATCATTC 
TGTCCTGCGA CGATGGACAT TATCAACAAG CTCATCGGAT TTGACGAGGA TGCTGCAAGT 
TTACTCGGCT TTGAGAATGC GGATGCCTTC TGCGGGAAGG TCAATCAAAT ATACAATGAA 
GATGGCGATT ATAACGTCAG TTTGGAACCT CTCTATGTTT TGTTTGCCGA TGTTGAAGAA 
GAAGGAAACT TTGACCCTGC AGCCATCATG AATGCCACAA CCGAGATGAT TAAATTCGCC 
GCCATGATCA TGCGTTCAGA CCACATTGGA GATGCCATTT CTAAAACATG CTACGTGTGG 
CAGGAATCTG CTGCAATGTT AAATATTTCA GAGGTTGACG ATTTATGTCA AACAGTATTG 
GCCTTTAATT CTGCAGAACT CCAAGATGTC TGCATCCACG AACTATTTCC CTGGATTGAG 
GACGTTTTTG ACCACCATGA TGATGATGAA ATGAGGGAAG AAGAAAACGA AATTGACTTT 
GATTTCACAG GGATTCTCGA AACGGTAGGC GAGGAATTCG GATTCGACTT ACACGATTCT 
CACCAAAGTT GCTCTCATCT TACTGGTATT CTCTTTGATG ATACCGTCAG CCAAAACTTT 
CCAATCGACA GCGTTGTAGT AAGGGGAATA AGAGCTGTTG GTAAGATAGT ATCACCCATA 
TTCTGTGATT TTGAATCATT TCATGACCAC CCAGATACTG TCTTGGAAGC TCCGTGTGCA 
ACTCCTGGAC AAAACTGCTT TGATTGGTCA AGCCGAGGAG AAGACTTCTT CTATGAGCGG 
GAAGACATTG GCTTTGTCAT TGGTACAATT TTCAGTTATA TTGGGGGCTA CAGAGATGGG 
GACCACCTTT GTCACCAAAT GGCCTTTGCA CTGGAGGATG ATGCACAACT TGACATGGTC 
GCAAATAAAA TGAAGGATAG TATCTTTCGA TTCTTCAACG ACCCGTCATT CTGTCCTGTG 
ACGATGGACA TTATTAACAG GCTCATCGGA TTTGACGAGG ATGCTGCAAG TTTACTCGGC 
TTTGAGAATG CGGATGCCTT CTGCGGAAAG GTCAATCAAA TATACAATGA AGATGGCGAT 
TATAACGTCA GTTTTGAGCC TCTCTATGTT TTGTTTGCCA ATGTTGAAGA AGAAGGAGAA 
TTTGACCCTG CAGCCATTAT AAATGCCACA ACCGAGATGA TTAAATTCGC CGCCATGATC 
ATGCGTTCAG ACCACATTGG AGATGCCATT TCTAAAACAT GCTACGTGTG GCAGGAATCT 
GCTGCAATGT TAAATATTTC AGAGATTGAT GATTTATGTC AATTGGTTCT TGAGGACAAT 
TCCGATGCTG TTTACCAAGT CTGCATGGGT GAACTTTTCC CACTTATTGA GTCAATTCTA 
GAAGACGAGA TGTATGATTT CAATGATAAT CATACAAACA CCGTTGTAGA TTTATCGAAC 
ATTGAGATCT GTCTCGATAA TGGCGAGGTG ACTTTTGAGG CACTTGAAAA TTTAGATATA 
TTGGCATATC TACTACAAAC CACACACAAG CTCTATAATT TAACCGTGTT CGACGAGAGC 
AACATTTGTA GAGCAGTGAC CAGCGTAGTA GAATCTAGAA ATGACATTCC GACACTGATA 
TCTGAATTTG CTGTGGAAAT GATGTCCCTC TTCATGCCGC TTGGTGCAGA AATATGCTCT 
TGTTGGGATC AAGTTTTTGA TGATCTCTTC TCGGACTCCG ACGACTCGGA ATTTGGCGTA 
AACCAATTCA ATGAGATAGT CCGGATTGCT GTGAACAGTA TTGGATTAGA CAGTCGTCAT 
GAACTCTGTG ACCATCTCAT TCATCTAAAG CCGGACAACA TGTCTGATTA CGCACAAACG 
GTGATTGAGG AAATCCTTGG TTTTACGGCT GATCCTGAGA AGTGTGCCTG TACCGCCAAT 
CGTGTCATCG ATTTCATCCT TCCCTTGGTT AATGTTTCAA TGGAGGAAAT CAATAACTAC 
CTTTACCAGT ACCTGGGATA CCATTCTGTG GATCACGTGT GTAGTTTCGT GGCCCAATCC 
TTCTCGGGAG TAATAGTCGA CCCTTCAAGC ATAAACTGTA ACAGCCAACA AGAACCAACA 
ACCAAAGCTA CGACCACAAC TCAATCATCA TTAGACAATA ATGGAAACTC AACTCAATCA 
TCAATAGACA ATACTGGAAA CTCAACTCAA TCACCAATAG ACAATACTGG AGACTCGAAT 
GAAACTTCGA CTGACGACGA AACCTGTCTC TTCATCACTG ACTGCGCCGG TGTATGCAAC 
GGTAACGCTA GTTTGGATTG CGCCCGAGAA TGCGGCGGCA GCGCGGCACT CGACTGCGCC 
TTCAAGTGCG GTGGCTCTGC GACCACGAAC GAATGTGGAT GGTGCGTCGG TGGAGCTACG 
GAAAGACCAA GCACTTTAGG TTTCGACCAG TGTGGTTCCT GCATTGGGGA AGGCACTGCG 
GTCATCGACT GCAATGGTGA CTGTGACGGT ACTGCTTACC GCGATGCCTG TCAAGTCTGC 
GTAGGTGGCA ATACGAGTGT GGCTGAGGAT GTAAGCGATC GTTTCTTGGA CTGCCTAGGT 
ATCTGTAATG GTAGTTTTGT GACCAATGAT TGTGATGAAT GCGTGCTGCC ATTCAGCGAT 
GGGACTATTT TCTCCACGGC GGATTGTAAC GGTGACTGCA ATGGATTTGC CGACATAAAT 
GAGTGTAGTT ACTGTGTTGG GGGTAATACC GGTAACTCTG TGGATACTGG ATTGTCTGCA 
TGTGGAGTCT GCAATACTAC GATCGAACCA GACTTCTGCC TCGGCTGTGA TGGCGTCGCT 
GATAGTGGCC TAGTGACCGA TGCGTGTGAT GTATGCGATG GGGATGGTAG CACTTGTTTC 
ACCGTGGACA ATATAGCTCC CTCTATCATC CCAGCTAACA CCGACTATCA GGTTAAACTT 
CGTGGTGCCG GATTCCAAGA TGCGACTACC AAACAGTGTA AGTTCCTGGA TAGCAGCGGT 
GAGCAGGTCA GCGTGGTCGA TATCAGCGAT GAGGTGGATG ACGAGTTCAA CTGCACCGCT 
AATCTTCCTC AAGGAACGTA TTCTGTTTAC CTTTCGACGG AGTCAACGGA ACTATCAGAA 
GCATTCGTCA CCCTCTATGT CTATTCAGAA GTTATAATAA ACAGCTTATC CCAGAGCGAG 
TTTGATATAG ATGATAGTGT GATTTCCATC TCTGTCAACA TGGTATCAAC AAGCGGAGCC 
TTTACGGCAT ACAAGGACTT GACCACTCCA AAAGCTATCG TCTTCACAGA GACCGAGAGA 
CTTTACTACG AAGGAACTTT TGGAGGAGAC AACGACGAGC TTCTCGAGTT TACGGGTCCG 
ATGCTGACTA CCTCGGCTCA AGTTGTCGTT TACCCGTCCT TCAACGGAAT CGACTACCTG 
GTCACCCCCG ATGATGCTGG TTTCACGATC ACCTACTACG CCCCTGCACC GGTCGCTCAG 
AGTCTTAAAT TCTCCCCTAC TGGACATCTT CTGGAACTCG AGTTCGACGT CGCAATCAAC 
GAAGACTCGG TGACATCATG TGATGCAGTA TTCATGAATA TCACCAGCCT GGGTGGTGCT 
GCTGCCCAGT GCTTTGCATT CCCTTACCTA AAAACTATCT ATATACTACC CTTCAGTTCG 
GCCAGTGTAA ATCTTATTGG ACCAGATGAT GAGCTAGTGC TGCGCGCTGA CGTTCTGAAA 
AAACGTGATG AGGAATACGC TCGGTACGCC AGCGGTACTC TTACCGCATC CTCCCCCGAC 
GAGGCGGTAA CCGTCTACGC GGCTCTGACC GGTACAGAAG CAATTAGCAG CTGCGGTAAT 
GTACGACTTA GTGCTCTGCA ATCCTTTGGA AACGCAGGAC GTTTTTTCAC CTATCAGTGG 
TCTGTGACCA GTGGCGGTTC GATCCCATCT GGCTTGACGG ATGATCTAAC AGCCGCAGCG 
GAGTCCGCCT ATGTCACTCT CGATGGATCC TACCTCGACG CGGACGTGCC GTATACATTC 
AGCATGACAG CAGAGAACTT CCTCGGAGGC AGTGACACAA AGACCTTGGA GGTGACTCGG 
TCTGCTCTCA ACAAACCAGA CGTCGTGATC TACCCGGAAA GAGTTGATCC TAATAGTGCC 
CTTATCTCGC AAGCGTTCTA CCTCCGCGCT GCTGTACGAT TCTACGAGGC CTGCGGTGGA 
GCTGAGCAGA TGGAGTATGC CTGGTCTGTA GATGATGATA GTATTGCTCT TAACCTACAA 
ACCATAAACA AACCACTCCT GTATGTGGCA GCTGGTTCTT TACCAGGCGA CTCCCAAGTG 
ACCTTCACAC TGGAGGTCAA CCGGGAAAGT GATCCCAGCA CGGTGACCAC GCAGACCGTG 
ACCATTACCA CGGTCTACAC CGACCTCGAC GCCCTAATCG ATGGCGGGAG TGAGCGGACC 
ATCGGCGTCG ACAGTGAGTC GTTCACACTG GATGGATCGT CGTCGTTTGA TCCCGACAGT 
GAAAATGAAG AGATGACCTA CCTCTGGACT TGTCAGAAGT CGAACACTGA GACAGGCAGC 
TACGAGCCTT GTGTATCAAG CGAGACACGT GAGGTGTTCC CGGCGGAAGC TGACTCCAAA 
ACAAGTACTC TCACGTTAGC TTCCAACAAT CTTTACTCGG ACTCCACCTA TCTGTTCACT 
CTGGAGATCA ACAAACTGTC AAGGACAGCA TCGACCAGTG TCACCATCCT TTCCAAGTCT 
GGCAATCCTC CAACGATCAG TCTCGATGAA TCCCTTCCTG AGAAGATAAA GAGCAGTAGT 
TCTCATACGC TACGAGTATT GATCAGCCAT ACATCAGATA TTACCATTGA CTGGGCTACT 
GTCGACATAG ATCTTGGATA TGGATACGTT GACTTTTCAC AGCTGAATAG TCAGTCATTC 
CTTATACAAG GAGATACATC ACCCTTCATC ACATACGCCT TTGTAACTAT CCCCACAGGA 
ACTTTGGAAG AGGATGTTGC CTACTCTTTC TCAGTGACGG TAACTGATAC CGAAGGACAG 
GTGTCAGCTA GTAAATTGAC TACCAACACG TATGGAGGAG TATCATCCTG TACATTCAGT 
TTGGGTGATG GTTCAACCAG CTATCAAGAA CTTGACGAGA TCACTCTCCT AACTCAGAAC 
TGCGTGACTG ATGAAGAAGC TTACCCTCTG TCTTACCAAG TGTTCCGTGA GAAGGTCGAA 
TCATCTGTCA CCCGTTACAA CGCGATGACG GGTGCATTGG CAGAAGCGAG CAGCACTATA 
TTGGGTCTGC CGTCCTTTAG CGACAACCAG AACACATTTG TTGTCAAGGT GTGCAATGAT 
TTTGGAAGCT GTTCCTCGTA CAGTCTTACA TTGGACGTCA CTCCCATCGC ATCTTTCAGT 
CAAGAACAGT TTGATGAAAG TATAACCAAT CTGGTAGAAC CACAAAAGCT CTCAGGGAAT 
TTCCTAGATG CGCTGGTAAA CTTCAACACC ATCACGTCAG TGGGTGTCGA CAGCACGAGT 
AGGAAACGGA GAGCAGTCTC GGAGACATCC AGCACAGCTA CTGAGCAGCT GGATTTACTT 
CAGAATGCCA TCTCGTCGAC GGTCCTTGAC ACGGCCACCG CCCAGACTCT GCTTGATCAG 
TCGGATGGCG TGGTCATTGC TGATCTCACA CTGTCTGATA TGGACATATA CCTGGATATT 
TTAATTGAGC TCGTAGCTGT TTTCAGTGAT GAAGGGCTTC CAGAGTCTAG CGCCGAGATC 
GTTCTTACAA AGACAAGCGA GATCGCTGCT GAACTCGACC CTCAATACAA CTCGGACATG 
CTTGCCAAAA TTACGACCCT GAGAAACACA TTAATCCAGG GCCAGCTGAG TCAGATCCCA 
CTTGGAGGGG ACCCTACGGA GACCAGGTCT GGTTCCGTTA CCACCAGGGC CTTCTATGCT 
ATCCCTAGTG ATACCCTCAG TACTGCATCC AGCAACACCT CCTTCAACGT CAAGTTCGGA 
AGCGAGATCG AAAGCTTATA TGGTGCAAGT TGGTCGTGTG CTTCTGGAAG CACTTGTTCT 
GGTGTTCAGA TCATGTTTAC GCAGTACGAC GACGAGGTTG ATTACTACTC GACTACGGAC 
GAGGATAAGG CGAACAGAGC ATCTTCTATC CTTGAAATCG ATCTGCTTGA CCCGAGTGAC 
AATTCTGAGT TGAGTATTAC TGGACTGAGC AGCCCCATAT CAATCAACAT GACCGCTTCA 
CGCCTCCAAT CCGACAAAGT CTATGACTGC TATTGGTGGG ACACTAGCAG CAGCTCTTGG 
AGCCAGGATG GACTTACGAC CGTCTACCAC TCCTCGGTCT TGTCTGAGTG CCAAACTACT 
CATCTTTCAG CCTTCACCCT GATCGCTGTT GATTCTCCTA CTACGATTAT GATGGCTACC 
ACGATAGTTG ATGACGCCAG TACTTCTACC GATGCAGTAG CCTCGACTGC GACAACGGAA 
ATCAGTGAGA GCGGCAGCAG TGGTCTAAGT TCTACCGTCA TCATCGTCAT TGCCGTGGTC 
ATATCTGTCG TTTTGATCCT AATTATTATC ATCGCCATCG TGGTCGTGAT GTCGAAGAAG 
TCCTCCAAGG TCGGTAATGC TGATATCGAG AACTCGAACG GCAATGGTGC CAGGAATGGG 
ACCAGCACCG CCGATGGACT CGCTCCTGTT GAATCTGCGA ACCAGGCATT CAATGCCCCA 
CCAACAGCAC CGGCCCACCC AACCCATACT GTTCAAGTGG TCGCTGCCCC AGCTTCGGCA 
CCTGCTTCTC AAGAGCCTAT CATGGACGTT GCAGACCTTC CTGGATCCCC ATCACCCCCT 
CCTGATGGAC CCCATATTGA TACCATCATA AAGGATGTTC AGAATGATAG TAGGGCCCAA 
ACTCCAATGG ATAGAGCCAT GACGCCCGTT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REJ8 ( XM_030976263.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030832123.1 Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X3, mRNA.

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CloneID	OSd71218
Clone ID Related Accession (Same CDS sequence)	XM_030976263.1
Accession Version	XM_030976263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	receptor for egg jelly 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145615.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC TTGTGGTGGT TGTAATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCCTC 
GTCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATAG GGCCAAAGCT TAGCTGGCTT TCCGATGGAG CAATGCAAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAAT 
TCTTGGTCCT TTCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCACTCGAAG ATTTGGATTT TAACTGGACC AACCCTTTAT TCGATGATAC TGCGAACATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGTA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGAGTGG TTCTAAACGT CATTGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG GTGCAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGGAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATTTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAC TGGATTCTCT CTATTGGAGA 
GGCAATATGA GCGAGATCGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGAGTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GACTGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AATTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGGTTGACGA TTTATGTAAA 
ACAGTATTGG CCTTTAATTC TGCAGAACTC CAAGATGTCT GCATCCACGA ACTATTTCCG 
TGGATTGAGG ATGTTTTTGA CCACCATGAG GATGATGAAA TGAGGGAAGA AGAAAACGAA 
ATTGACTTTG ATTTCACAGG GATTCTCGAA ACGGTAGGCG AGGAATTCGG ATTCGACTTT 
CACGATTCTC ACCAAAGTTG CTCTCATCTT ACTGGTATTC TCTTTGATGA TACCGTCAGC 
CAAAACTTTC CAATCGACAG CGTTGTGGTA AGGGGAATAA GAGCTGTTGG TAAGATAGTA 
TCACCCATAT TCTGTGATTT TGAATCATTT CATGACCACC CAGATACTGT CTTGGAAGCT 
CCGTGTGCAA CTCCTGGACA AAACTGCTTT GATTGGTCAA GCCGAGGAGA AGACTTCTTC 
TATGAGCGAG AAGACATTGG CTTTGTCATT GGTACAATTT TCAGTTATAT CGGAGGCTAC 
AGAGATGGGG ACCACCTTTG TCACCAAATG GCCTTTGCAC TGGATGATGA TGAACAACTT 
GACATGGTAG CAAATAAAAT GAAGGATAGT ATCTTCCGAT TCTTCAACGA CCCATCATTC 
TGTCCTGCGA CGATGGACAT TATCAACAAG CTCATCGGAT TTGACGAGGA TGCTGCAAGT 
TTACTCGGCT TTGAGAATGC GGATGCCTTC TGCGGGAAGG TCAATCAAAT ATACAATGAA 
GATGGCGATT ATAACGTCAG TTTGGAACCT CTCTATGTTT TGTTTGCCGA TGTTGAAGAA 
GAAGGAAACT TTGACCCTGC AGCCATCATG AATGCCACAA CCGAGATGAT TAAATTCGCC 
GCCATGATCA TGCGTTCAGA CCACATTGGA GATGCCATTT CTAAAACATG CTACGTGTGG 
CAGGAATCTG CTGCAATGTT AAATATTTCA GAGATTGATG ATTTATGTCA ATTGGTTCTT 
GAGGACAATT CCGATGCTGT TTACCAAGTC TGCATGGGTG AACTTTTCCC ACTTATTGAG 
TCAATTCTAG AAGACGAGAT GTATGATTTC AATGATAATC ATACAAACAC CGTTGTAGAT 
TTATCGAACA TTGAGATCTG TCTCGATAAT GGCGAGGTGA CTTTTGAGGC ACTTGAAAAT 
TTAGATATAT TGGCATATCT ACTACAAACC ACACACAAGC TCTATAATTT AACCGTGTTC 
GACGAGAGCA ACATTTGTAG AGCAGTGACC AGCGTAGTAG AATCTAGAAA TGACATTCCG 
ACACTGATAT CTGAATTTGC TGTGGAAATG ATGTCCCTCT TCATGCCGCT TGGTGCAGAA 
ATATGCTCTT GTTGGGATCA AGTTTTTGAT GATCTCTTCT CGGACTCCGA CGACTCGGAA 
TTTGGCGTAA ACCAATTCAA TGAGATAGTC CGGATTGCTG TGAACAGTAT TGGATTAGAC 
AGTCGTCATG AACTCTGTGA CCATCTCATT CATCTAAAGC CGGACAACAT GTCTGATTAC 
GCACAAACGG TGATTGAGGA AATCCTTGGT TTTACGGCTG ATCCTGAGAA GTGTGCCTGT 
ACCGCCAATC GTGTCATCGA TTTCATCCTT CCCTTGGTTA ATGTTTCAAT GGAGGAAATC 
AATAACTACC TTTACCAGTA CCTGGGATAC CATTCTGTGG ATCACGTGTG TAGTTTCGTG 
GCCCAATCCT TCTCGGGAGT AATAGTCGAC CCTTCAAGCA TAAACTGTAA CAGCCAACAA 
GAACCAACAA CCAAAGCTAC GACCACAACT CAATCATCAT TAGACAATAA TGGAAACTCA 
ACTCAATCAT CAATAGACAA TACTGGAAAC TCAACTCAAT CACCAATAGA CAATACTGGA 
GACTCGAATG AAACTTCGAC TGACGACGAA ACCTGTCTCT TCATCACTGA CTGCGCCGGT 
GTATGCAACG GTAACGCTAG TTTGGATTGC GCCCGAGAAT GCGGCGGCAG CGCGGCACTC 
GACTGCGCCT TCAAGTGCGG TGGCTCTGCG ACCACGAACG AATGTGGATG GTGCGTCGGT 
GGAGCTACGG AAAGACCAAG CACTTTAGGT TTCGACCAGT GTGGTTCCTG CATTGGGGAA 
GGCACTGCGG TCATCGACTG CAATGGTGAC TGTGACGGTA CTGCTTACCG CGATGCCTGT 
CAAGTCTGCG TAGGTGGCAA TACGAGTGTG GCTGAGGATG TAAGCGATCG TTTCTTGGAC 
TGCCTAGGTA TCTGTAATGG TAGTTTTGTG ACCAATGATT GTGATGAATG CGTGCTGCCA 
TTCAGCGATG GGACTATTTT CTCCACGGCG GATTGTAACG GTGACTGCAA TGGATTTGCC 
GACATAAATG AGTGTAGTTA CTGTGTTGGG GGTAATACCG GTAACTCTGT GGATACTGGA 
TTGTCTGCAT GTGGAGTCTG CAATACTACG ATCGAACCAG ACTTCTGCCT CGGCTGTGAT 
GGCGTCGCTG ATAGTGGCCT AGTGACCGAT GCGTGTGATG TATGCGATGG GGATGGTAGC 
ACTTGTTTCA CCGTGGACAA TATAGCTCCC TCTATCATCC CAGCTAACAC CGACTATCAG 
GTTAAACTTC GTGGTGCCGG ATTCCAAGAT GCGACTACCA AACAGTGTAA GTTCCTGGAT 
AGCAGCGGTG AGCAGGTCAG CGTGGTCGAT ATCAGCGATG AGGTGGATGA CGAGTTCAAC 
TGCACCGCTA ATCTTCCTCA AGGAACGTAT TCTGTTTACC TTTCGACGGA GTCAACGGAA 
CTATCAGAAG CATTCGTCAC CCTCTATGTC TATTCAGAAG TTATAATAAA CAGCTTATCC 
CAGAGCGAGT TTGATATAGA TGATAGTGTG ATTTCCATCT CTGTCAACAT GGTATCAACA 
AGCGGAGCCT TTACGGCATA CAAGGACTTG ACCACTCCAA AAGCTATCGT CTTCACAGAG 
ACCGAGAGAC TTTACTACGA AGGAACTTTT GGAGGAGACA ACGACGAGCT TCTCGAGTTT 
ACGGGTCCGA TGCTGACTAC CTCGGCTCAA GTTGTCGTTT ACCCGTCCTT CAACGGAATC 
GACTACCTGG TCACCCCCGA TGATGCTGGT TTCACGATCA CCTACTACGC CCCTGCACCG 
GTCGCTCAGA GTCTTAAATT CTCCCCTACT GGACATCTTC TGGAACTCGA GTTCGACGTC 
GCAATCAACG AAGACTCGGT GACATCATGT GATGCAGTAT TCATGAATAT CACCAGCCTG 
GGTGGTGCTG CTGCCCAGTG CTTTGCATTC CCTTACCTAA AAACTATCTA TATACTACCC 
TTCAGTTCGG CCAGTGTAAA TCTTATTGGA CCAGATGATG AGCTAGTGCT GCGCGCTGAC 
GTTCTGAAAA AACGTGATGA GGAATACGCT CGGTACGCCA GCGGTACTCT TACCGCATCC 
TCCCCCGACG AGGCGGTAAC CGTCTACGCG GCTCTGACCG GTACAGAAGC AATTAGCAGC 
TGCGGTAATG TACGACTTAG TGCTCTGCAA TCCTTTGGAA ACGCAGGACG TTTTTTCACC 
TATCAGTGGT CTGTGACCAG TGGCGGTTCG ATCCCATCTG GCTTGACGGA TGATCTAACA 
GCCGCAGCGG AGTCCGCCTA TGTCACTCTC GATGGATCCT ACCTCGACGC GGACGTGCCG 
TATACATTCA GCATGACAGC AGAGAACTTC CTCGGAGGCA GTGACACAAA GACCTTGGAG 
GTGACTCGGT CTGCTCTCAA CAAACCAGAC GTCGTGATCT ACCCGGAAAG AGTTGATCCT 
AATAGTGCCC TTATCTCGCA AGCGTTCTAC CTCCGCGCTG CTGTACGATT CTACGAGGCC 
TGCGGTGGAG CTGAGCAGAT GGAGTATGCC TGGTCTGTAG ATGATGATAG TATTGCTCTT 
AACCTACAAA CCATAAACAA ACCACTCCTG TATGTGGCAG CTGGTTCTTT ACCAGGCGAC 
TCCCAAGTGA CCTTCACACT GGAGGTCAAC CGGGAAAGTG ATCCCAGCAC GGTGACCACG 
CAGACCGTGA CCATTACCAC GGTCTACACC GACCTCGACG CCCTAATCGA TGGCGGGAGT 
GAGCGGACCA TCGGCGTCGA CAGTGAGTCG TTCACACTGG ATGGATCGTC GTCGTTTGAT 
CCCGACAGTG AAAATGAAGA GATGACCTAC CTCTGGACTT GTCAGAAGTC GAACACTGAG 
ACAGGCAGCT ACGAGCCTTG TGTATCAAGC GAGACACGTG AGGTGTTCCC GGCGGAAGCT 
GACTCCAAAA CAAGTACTCT CACGTTAGCT TCCAACAATC TTTACTCGGA CTCCACCTAT 
CTGTTCACTC TGGAGATCAA CAAACTGTCA AGGACAGCAT CGACCAGTGT CACCATCCTT 
TCCAAGTCTG GCAATCCTCC AACGATCAGT CTCGATGAAT CCCTTCCTGA GAAGATAAAG 
AGCAGTAGTT CTCATACGCT ACGAGTATTG ATCAGCCATA CATCAGATAT TACCATTGAC 
TGGGCTACTG TCGACATAGA TCTTGGATAT GGATACGTTG ACTTTTCACA GCTGAATAGT 
CAGTCATTCC TTATACAAGG AGATACATCA CCCTTCATCA CATACGCCTT TGTAACTATC 
CCCACAGGAA CTTTGGAAGA GGATGTTGCC TACTCTTTCT CAGTGACGGT AACTGATACC 
GAAGGACAGG TGTCAGCTAG TAAATTGACT ACCAACACGT ATGGAGGAGT ATCATCCTGT 
ACATTCAGTT TGGGTGATGG TTCAACCAGC TATCAAGAAC TTGACGAGAT CACTCTCCTA 
ACTCAGAACT GCGTGACTGA TGAAGAAGCT TACCCTCTGT CTTACCAAGT GTTCCGTGAG 
AAGGTCGAAT CATCTGTCAC CCGTTACAAC GCGATGACGG GTGCATTGGC AGAAGCGAGC 
AGCACTATAT TGGGTCTGCC GTCCTTTAGC GACAACCAGA ACACATTTGT TGTCAAGGTG 
TGCAATGATT TTGGAAGCTG TTCCTCGTAC AGTCTTACAT TGGACGTCAC TCCCATCGCA 
TCTTTCAGTC AAGAACAGTT TGATGAAAGT ATAACCAATC TGGTAGAACC ACAAAAGCTC 
TCAGGGAATT TCCTAGATGC GCTGGTAAAC TTCAACACCA TCACGTCAGT GGGTGTCGAC 
AGCACGAGTA GGAAACGGAG AGCAGTCTCG GAGACATCCA GCACAGCTAC TGAGCAGCTG 
GATTTACTTC AGAATGCCAT CTCGTCGACG GTCCTTGACA CGGCCACCGC CCAGACTCTG 
CTTGATCAGT CGGATGGCGT GGTCATTGCT GATCTCACAC TGTCTGATAT GGACATATAC 
CTGGATATTT TAATTGAGCT CGTAGCTGTT TTCAGTGATG AAGGGCTTCC AGAGTCTAGC 
GCCGAGATCG TTCTTACAAA GACAAGCGAG ATCGCTGCTG AACTCGACCC TCAATACAAC 
TCGGACATGC TTGCCAAAAT TACGACCCTG AGAAACACAT TAATCCAGGG CCAGCTGAGT 
CAGATCCCAC TTGGAGGGGA CCCTACGGAG ACCAGGTCTG GTTCCGTTAC CACCAGGGCC 
TTCTATGCTA TCCCTAGTGA TACCCTCAGT ACTGCATCCA GCAACACCTC CTTCAACGTC 
AAGTTCGGAA GCGAGATCGA AAGCTTATAT GGTGCAAGTT GGTCGTGTGC TTCTGGAAGC 
ACTTGTTCTG GTGTTCAGAT CATGTTTACG CAGTACGACG ACGAGGTTGA TTACTACTCG 
ACTACGGACG AGGATAAGGC GAACAGAGCA TCTTCTATCC TTGAAATCGA TCTGCTTGAC 
CCGAGTGACA ATTCTGAGTT GAGTATTACT GGACTGAGCA GCCCCATATC AATCAACATG 
ACCGCTTCAC GCCTCCAATC CGACAAAGTC TATGACTGCT ATTGGTGGGA CACTAGCAGC 
AGCTCTTGGA GCCAGGATGG ACTTACGACC GTCTACCACT CCTCGGTCTT GTCTGAGTGC 
CAAACTACTC ATCTTTCAGC CTTCACCCTG ATCGCTGTTG ATTCTCCTAC TACGATTATG 
ATGGCTACCA CGATAGTTGA TGACGCCAGT ACTTCTACCG ATGCAGTAGC CTCGACTGCG 
ACAACGGAAA TCAGTGAGAG CGGCAGCAGT GGTCTAAGTT CTACCGTCAT CATCGTCATT 
GCCGTGGTCA TATCTGTCGT TTTGATCCTA ATTATTATCA TCGCCATCGT GGTCGTGATG 
TCGAAGAAGT CCTCCAAGGT CGGTAATGCT GATATCGAGA ACTCGAACGG CAATGGTGCC 
AGGAATGGGA CCAGCACCGC CGATGGACTC GCTCCTGTTG AATCTGCGAA CCAGGCATTC 
AATGCCCCAC CAACAGCACC GGCCCACCCA ACCCATACTG TTCAAGTGGT CGCTGCCCCA 
GCTTCGGCAC CTGCTTCTCA AGAGCCTATC ATGGACGTTG CAGACCTTCC TGGATCCCCA 
TCACCCCCTC CTGATGGACC CCATATTGAT ACCATCATAA AGGATGTTCA GAATGATAGT 
AGGGCCCAAA CTCCAATGGA TAGAGCCATG ACGCCCGTTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030832123.1
CDS	213..10037
Translation	MKALVVVVIFSCLVAGAARAEESTEWQVEWTTEPAYNLSLVEPFTVPGYLLPDLNISDIISTVGMMYQAEDLVHAIGSLCVEIGPKLSWLSDGAMQELCDPVLEALRNGSYAEAYSICQNSWSFLETLIGGEEGGLEGEGEIDHGPIRRKRWGLSVPSIELPPLPSSPIQAVLNITVPFSPLEDLDFNWTNPLFDDTANMVENIGLIPDLNRNWSFPIFDGTGIEVDFPYPEFSDKYGICSSLAGLISYNQPSSPSHGSIFGVVLNVIVAEQVRSIPGKCLSFFDGEEGSGDIDDDPSFKHEPGAMIWAFATLGGFDDIEQICNVSSWALLMRDEGKFELFNELVNQITTSIYSVISSRSRCIEFADSLHANIGLAPGNYSHFTSSEALCDEILDSLYWRGNMSEIDFYNYDSMDEKDAFIYLLSIVEILSHKSDMASLEFLCRMSPYADEMFCTSLAYNDYRDVSREDIIEHCLRYVAFDGFEFDFLALLNAIGAEFEFDFYDFDQTCSHLTDIVFNSVIRQQFTIDNMIQKGMKAIGRVLSPIFCDLHSFRNGSLDSLEAPCGTPGRGCFNWTRPGEDYFFDRLDEGFIVGTIFSYLGGYRDGDHICHQMAFALDDETVLDMVAGKLRDSVYRLFDDVPFCSDTIGLIEKLIGVDEHAAQMIGFNSTDAFCGEISKIYHQGGNYNFSFVPLLDLFTNHSHGLEHFVTSESIMHVTTEMMKFAAMIMHSDNIGDAISKSCYMWQDSGPGITMPLFEVDDLCQVVLALNPAELQDVCIHDIIPWIEDLFDHHHDDEERGEENEIDFDFTGILETVGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFYDLPASHLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALEDDAQLDMVANKMKDSIFRFFNDPSFCPVTMDLINRLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSLEPLYVLFANVEEEGEFDPAAIINATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEVDDLCKTVLAFNSAELQDVCIHELFPWIEDVFDHHEDDEMREEENEIDFDFTGILETVGEEFGFDFHDSHQSCSHLTGILFDDTVSQNFPIDSVVVRGIRAVGKIVSPIFCDFESFHDHPDTVLEAPCATPGQNCFDWSSRGEDFFYEREDIGFVIGTIFSYIGGYRDGDHLCHQMAFALDDDEQLDMVANKMKDSIFRFFNDPSFCPATMDIINKLIGFDEDAASLLGFENADAFCGKVNQIYNEDGDYNVSLEPLYVLFADVEEEGNFDPAAIMNATTEMIKFAAMIMRSDHIGDAISKTCYVWQESAAMLNISEIDDLCQLVLEDNSDAVYQVCMGELFPLIESILEDEMYDFNDNHTNTVVDLSNIEICLDNGEVTFEALENLDILAYLLQTTHKLYNLTVFDESNICRAVTSVVESRNDIPTLISEFAVEMMSLFMPLGAEICSCWDQVFDDLFSDSDDSEFGVNQFNEIVRIAVNSIGLDSRHELCDHLIHLKPDNMSDYAQTVIEEILGFTADPEKCACTANRVIDFILPLVNVSMEEINNYLYQYLGYHSVDHVCSFVAQSFSGVIVDPSSINCNSQQEPTTKATTTTQSSLDNNGNSTQSSIDNTGNSTQSPIDNTGDSNETSTDDETCLFITDCAGVCNGNASLDCARECGGSAALDCAFKCGGSATTNECGWCVGGATERPSTLGFDQCGSCIGEGTAVIDCNGDCDGTAYRDACQVCVGGNTSVAEDVSDRFLDCLGICNGSFVTNDCDECVLPFSDGTIFSTADCNGDCNGFADINECSYCVGGNTGNSVDTGLSACGVCNTTIEPDFCLGCDGVADSGLVTDACDVCDGDGSTCFTVDNIAPSIIPANTDYQVKLRGAGFQDATTKQCKFLDSSGEQVSVVDISDEVDDEFNCTANLPQGTYSVYLSTESTELSEAFVTLYVYSEVIINSLSQSEFDIDDSVISISVNMVSTSGAFTAYKDLTTPKAIVFTETERLYYEGTFGGDNDELLEFTGPMLTTSAQVVVYPSFNGIDYLVTPDDAGFTITYYAPAPVAQSLKFSPTGHLLELEFDVAINEDSVTSCDAVFMNITSLGGAAAQCFAFPYLKTIYILPFSSASVNLIGPDDELVLRADVLKKRDEEYARYASGTLTASSPDEAVTVYAALTGTEAISSCGNVRLSALQSFGNAGRFFTYQWSVTSGGSIPSGLTDDLTAAAESAYVTLDGSYLDADVPYTFSMTAENFLGGSDTKTLEVTRSALNKPDVVIYPERVDPNSALISQAFYLRAAVRFYEACGGAEQMEYAWSVDDDSIALNLQTINKPLLYVAAGSLPGDSQVTFTLEVNRESDPSTVTTQTVTITTVYTDLDALIDGGSERTIGVDSESFTLDGSSSFDPDSENEEMTYLWTCQKSNTETGSYEPCVSSETREVFPAEADSKTSTLTLASNNLYSDSTYLFTLEINKLSRTASTSVTILSKSGNPPTISLDESLPEKIKSSSSHTLRVLISHTSDITIDWATVDIDLGYGYVDFSQLNSQSFLIQGDTSPFITYAFVTIPTGTLEEDVAYSFSVTVTDTEGQVSASKLTTNTYGGVSSCTFSLGDGSTSYQELDEITLLTQNCVTDEEAYPLSYQVFREKVESSVTRYNAMTGALAEASSTILGLPSFSDNQNTFVVKVCNDFGSCSSYSLTLDVTPIASFSQEQFDESITNLVEPQKLSGNFLDALVNFNTITSVGVDSTSRKRRAVSETSSTATEQLDLLQNAISSTVLDTATAQTLLDQSDGVVIADLTLSDMDIYLDILIELVAVFSDEGLPESSAEIVLTKTSEIAAELDPQYNSDMLAKITTLRNTLIQGQLSQIPLGGDPTETRSGSVTTRAFYAIPSDTLSTASSNTSFNVKFGSEIESLYGASWSCASGSTCSGVQIMFTQYDDEVDYYSTTDEDKANRASSILEIDLLDPSDNSELSITGLSSPISINMTASRLQSDKVYDCYWWDTSSSSWSQDGLTTVYHSSVLSECQTTHLSAFTLIAVDSPTTIMMATTIVDDASTSTDAVASTATTEISESGSSGLSSTVIIVIAVVISVVLILIIIIAIVVVMSKKSSKVGNADIENSNGNGARNGTSTADGLAPVESANQAFNAPPTAPAHPTHTVQVVAAPASAPASQEPIMDVADLPGSPSPPPDGPHIDTIIKDVQNDSRAQTPMDRAMTPVE

Target ORF information:

RefSeq Version	XM_030976263.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus receptor for egg jelly 8 (REJ8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030976263.1

ORF Insert Sequence:

ATGAAGGCAC TTGTGGTGGT TGTAATTTTC TCTTGTCTGG TCGCCGGGGC AGCCCGAGCT 
GAGGAGTCCA CCGAATGGCA GGTCGAATGG ACCACGGAAC CAGCGTACAA CCTCAGCCTC 
GTCGAACCGT TCACCGTCCC CGGATATCTT CTACCAGACC TTAACATAAG TGACATCATA 
TCTACAGTAG GCATGATGTA CCAAGCTGAA GATCTCGTCC ATGCGATCGG TTCCCTGTGC 
GTTGAGATAG GGCCAAAGCT TAGCTGGCTT TCCGATGGAG CAATGCAAGA GCTTTGTGAT 
CCTGTCCTTG AGGCTCTCCG GAATGGATCC TATGCTGAAG CCTACTCTAT TTGTCAGAAT 
TCTTGGTCCT TTCTTGAAAC ACTCATTGGA GGTGAAGAAG GTGGACTTGA AGGTGAAGGT 
GAGATTGACC ACGGTCCAAT AAGACGAAAG CGTTGGGGAT TGTCAGTTCC CTCGATTGAA 
CTTCCGCCTC TCCCATCGTC TCCCATACAA GCTGTTCTGA ACATAACTGT CCCGTTTTCA 
CCACTCGAAG ATTTGGATTT TAACTGGACC AACCCTTTAT TCGATGATAC TGCGAACATG 
GTCGAGAATA TTGGACTCAT CCCAGACTTG AATAGAAATT GGTCGTTTCC AATATTCGAC 
GGTACAGGTA TTGAAGTTGA TTTCCCATAT CCCGAATTTT CCGACAAGTA TGGGATATGT 
TCAAGTCTGG CGGGGCTCAT TTCATACAAC CAGCCATCAT CTCCCAGTCA TGGCAGTATC 
TTTGGAGTGG TTCTAAACGT CATTGTTGCC GAACAGGTTA GATCAATCCC TGGTAAATGT 
CTGTCTTTCT TTGACGGTGA AGAGGGTAGT GGTGACATTG ACGACGATCC GTCCTTCAAA 
CACGAACCTG GTGCAATGAT ATGGGCATTC GCAACGCTTG GTGGCTTCGA TGATATTGAG 
CAGATCTGCA ATGTTTCTAG TTGGGCGCTT CTTATGAGAG ATGAGGGGAA GTTTGAACTG 
TTCAATGAAC TCGTAAACCA AATAACTACA AGCATTTACA GTGTCATTAG CAGCAGAAGC 
CGGTGTATTG AATTTGCAGA TAGCCTCCAT GCTAATATTG GCCTAGCACC AGGAAACTAC 
TCTCATTTCA CCAGTAGTGA AGCGCTTTGT GATGAAATAC TGGATTCTCT CTATTGGAGA 
GGCAATATGA GCGAGATCGA TTTTTACAAT TATGATAGTA TGGATGAAAA AGATGCTTTC 
ATTTATCTGC TCTCTATTGT AGAAATACTT TCCCATAAAT CGGACATGGC ATCCCTTGAA 
TTTCTCTGCC GAATGTCTCC GTATGCTGAT GAAATGTTCT GTACTTCCTT GGCATACAAT 
GACTACAGGG ATGTTTCAAG GGAAGACATT ATTGAGCATT GTCTGAGATA CGTGGCATTT 
GATGGCTTTG AATTTGATTT CCTAGCCCTT CTCAACGCAA TTGGCGCCGA ATTCGAGTTC 
GACTTTTACG ACTTCGATCA AACTTGCTCA CACCTCACTG ATATCGTCTT TAATAGCGTA 
ATCAGACAAC AATTTACAAT CGACAACATG ATCCAGAAGG GGATGAAAGC TATTGGTAGG 
GTCCTATCAC CAATCTTCTG TGATTTGCAC TCATTCCGAA ACGGTTCGCT TGATTCCTTG 
GAAGCCCCAT GCGGAACTCC GGGACGAGGT TGCTTCAACT GGACAAGACC AGGCGAGGAC 
TACTTCTTTG ATCGTTTGGA TGAAGGCTTT ATTGTCGGGA CCATCTTCAG CTATCTCGGA 
GGCTACAGGG ATGGAGATCA CATCTGCCAC CAGATGGCCT TTGCACTGGA TGATGAGACA 
GTACTCGATA TGGTGGCTGG TAAATTGCGG GACAGTGTCT ACAGACTGTT CGATGATGTA 
CCATTCTGTT CTGACACAAT CGGCCTCATC GAAAAACTCA TAGGTGTCGA CGAACATGCT 
GCTCAGATGA TCGGCTTCAA TAGTACGGAT GCCTTCTGCG GGGAAATATC CAAAATCTAC 
CATCAGGGCG GCAACTATAA CTTTAGTTTT GTACCTCTCT TAGACCTCTT TACAAACCAT 
AGTCACGGAC TGGAACACTT TGTTACGTCT GAGTCTATAA TGCACGTCAC GACTGAGATG 
ATGAAATTCG CTGCCATGAT AATGCACTCG GACAACATTG GAGATGCCAT TTCCAAATCG 
TGTTACATGT GGCAGGACTC TGGTCCAGGG ATTACTATGC CTTTATTTGA GGTTGATGAT 
TTATGTCAAG TTGTCTTGGC CCTTAATCCT GCAGAACTCC AAGATGTCTG CATCCATGAC 
ATAATTCCGT GGATTGAGGA CCTTTTTGAT CACCATCATG ATGATGAAGA AAGGGGAGAA 
GAAAACGAAA TTGACTTTGA TTTCACAGGG ATTTTGGAAA CGGTAGGCGA AGAATTCGGA 
TTCGACTTTC ACGATTCTCA CCAAAGTTGC TCTCATCTTA CTGGTATTCT CTTTGATGAT 
ACCGTCAGCC AAAACTTTCC AATCGACAGC GTTGTGGTAA GGGGAATAAG AGCTGTTGGT 
AAGATAGTAT CACCCATATT CTGTGATTTT GAATCATTCT ATGACCTCCC AGCGAGTCAC 
TTGGAAGCTC CGTGCGCAAC TCCTGGACAA AATTGCTTTG ATTGGTCAAG CCGAGGAGAA 
GACTTCTTCT ATGAGCGAGA AGATATTGGC TTTGTCATTG GTACAATTTT CAGTTATATT 
GGAGGCTACA GAGATGGGGA CCACCTTTGT CACCAAATGG CCTTTGCACT GGAGGATGAT 
GCACAACTTG ACATGGTCGC AAATAAAATG AAGGATAGTA TCTTTCGATT CTTCAACGAC 
CCGTCATTCT GTCCTGTGAC GATGGACCTT ATCAACAGGC TCATCGGATT TGACGAGGAT 
GCTGCAAGTT TACTCGGCTT TGAGAATGCG GATGCCTTCT GCGGGAAGGT CAATCAAATA 
TACAATGAAG ATGGCGATTA TAACGTCAGT TTGGAGCCTC TCTATGTTTT GTTTGCCAAT 
GTTGAAGAAG AAGGAGAATT TGACCCTGCA GCCATTATAA ATGCCACAAC CGAGATGATT 
AAATTCGCCG CCATGATCAT GCGTTCAGAC CACATTGGGG ATGCCATTTC TAAAACATGC 
TACGTGTGGC AGGAATCTGC TGCAATGTTA AATATTTCAG AGGTTGACGA TTTATGTAAA 
ACAGTATTGG CCTTTAATTC TGCAGAACTC CAAGATGTCT GCATCCACGA ACTATTTCCG 
TGGATTGAGG ATGTTTTTGA CCACCATGAG GATGATGAAA TGAGGGAAGA AGAAAACGAA 
ATTGACTTTG ATTTCACAGG GATTCTCGAA ACGGTAGGCG AGGAATTCGG ATTCGACTTT 
CACGATTCTC ACCAAAGTTG CTCTCATCTT ACTGGTATTC TCTTTGATGA TACCGTCAGC 
CAAAACTTTC CAATCGACAG CGTTGTGGTA AGGGGAATAA GAGCTGTTGG TAAGATAGTA 
TCACCCATAT TCTGTGATTT TGAATCATTT CATGACCACC CAGATACTGT CTTGGAAGCT 
CCGTGTGCAA CTCCTGGACA AAACTGCTTT GATTGGTCAA GCCGAGGAGA AGACTTCTTC 
TATGAGCGAG AAGACATTGG CTTTGTCATT GGTACAATTT TCAGTTATAT CGGAGGCTAC 
AGAGATGGGG ACCACCTTTG TCACCAAATG GCCTTTGCAC TGGATGATGA TGAACAACTT 
GACATGGTAG CAAATAAAAT GAAGGATAGT ATCTTCCGAT TCTTCAACGA CCCATCATTC 
TGTCCTGCGA CGATGGACAT TATCAACAAG CTCATCGGAT TTGACGAGGA TGCTGCAAGT 
TTACTCGGCT TTGAGAATGC GGATGCCTTC TGCGGGAAGG TCAATCAAAT ATACAATGAA 
GATGGCGATT ATAACGTCAG TTTGGAACCT CTCTATGTTT TGTTTGCCGA TGTTGAAGAA 
GAAGGAAACT TTGACCCTGC AGCCATCATG AATGCCACAA CCGAGATGAT TAAATTCGCC 
GCCATGATCA TGCGTTCAGA CCACATTGGA GATGCCATTT CTAAAACATG CTACGTGTGG 
CAGGAATCTG CTGCAATGTT AAATATTTCA GAGATTGATG ATTTATGTCA ATTGGTTCTT 
GAGGACAATT CCGATGCTGT TTACCAAGTC TGCATGGGTG AACTTTTCCC ACTTATTGAG 
TCAATTCTAG AAGACGAGAT GTATGATTTC AATGATAATC ATACAAACAC CGTTGTAGAT 
TTATCGAACA TTGAGATCTG TCTCGATAAT GGCGAGGTGA CTTTTGAGGC ACTTGAAAAT 
TTAGATATAT TGGCATATCT ACTACAAACC ACACACAAGC TCTATAATTT AACCGTGTTC 
GACGAGAGCA ACATTTGTAG AGCAGTGACC AGCGTAGTAG AATCTAGAAA TGACATTCCG 
ACACTGATAT CTGAATTTGC TGTGGAAATG ATGTCCCTCT TCATGCCGCT TGGTGCAGAA 
ATATGCTCTT GTTGGGATCA AGTTTTTGAT GATCTCTTCT CGGACTCCGA CGACTCGGAA 
TTTGGCGTAA ACCAATTCAA TGAGATAGTC CGGATTGCTG TGAACAGTAT TGGATTAGAC 
AGTCGTCATG AACTCTGTGA CCATCTCATT CATCTAAAGC CGGACAACAT GTCTGATTAC 
GCACAAACGG TGATTGAGGA AATCCTTGGT TTTACGGCTG ATCCTGAGAA GTGTGCCTGT 
ACCGCCAATC GTGTCATCGA TTTCATCCTT CCCTTGGTTA ATGTTTCAAT GGAGGAAATC 
AATAACTACC TTTACCAGTA CCTGGGATAC CATTCTGTGG ATCACGTGTG TAGTTTCGTG 
GCCCAATCCT TCTCGGGAGT AATAGTCGAC CCTTCAAGCA TAAACTGTAA CAGCCAACAA 
GAACCAACAA CCAAAGCTAC GACCACAACT CAATCATCAT TAGACAATAA TGGAAACTCA 
ACTCAATCAT CAATAGACAA TACTGGAAAC TCAACTCAAT CACCAATAGA CAATACTGGA 
GACTCGAATG AAACTTCGAC TGACGACGAA ACCTGTCTCT TCATCACTGA CTGCGCCGGT 
GTATGCAACG GTAACGCTAG TTTGGATTGC GCCCGAGAAT GCGGCGGCAG CGCGGCACTC 
GACTGCGCCT TCAAGTGCGG TGGCTCTGCG ACCACGAACG AATGTGGATG GTGCGTCGGT 
GGAGCTACGG AAAGACCAAG CACTTTAGGT TTCGACCAGT GTGGTTCCTG CATTGGGGAA 
GGCACTGCGG TCATCGACTG CAATGGTGAC TGTGACGGTA CTGCTTACCG CGATGCCTGT 
CAAGTCTGCG TAGGTGGCAA TACGAGTGTG GCTGAGGATG TAAGCGATCG TTTCTTGGAC 
TGCCTAGGTA TCTGTAATGG TAGTTTTGTG ACCAATGATT GTGATGAATG CGTGCTGCCA 
TTCAGCGATG GGACTATTTT CTCCACGGCG GATTGTAACG GTGACTGCAA TGGATTTGCC 
GACATAAATG AGTGTAGTTA CTGTGTTGGG GGTAATACCG GTAACTCTGT GGATACTGGA 
TTGTCTGCAT GTGGAGTCTG CAATACTACG ATCGAACCAG ACTTCTGCCT CGGCTGTGAT 
GGCGTCGCTG ATAGTGGCCT AGTGACCGAT GCGTGTGATG TATGCGATGG GGATGGTAGC 
ACTTGTTTCA CCGTGGACAA TATAGCTCCC TCTATCATCC CAGCTAACAC CGACTATCAG 
GTTAAACTTC GTGGTGCCGG ATTCCAAGAT GCGACTACCA AACAGTGTAA GTTCCTGGAT 
AGCAGCGGTG AGCAGGTCAG CGTGGTCGAT ATCAGCGATG AGGTGGATGA CGAGTTCAAC 
TGCACCGCTA ATCTTCCTCA AGGAACGTAT TCTGTTTACC TTTCGACGGA GTCAACGGAA 
CTATCAGAAG CATTCGTCAC CCTCTATGTC TATTCAGAAG TTATAATAAA CAGCTTATCC 
CAGAGCGAGT TTGATATAGA TGATAGTGTG ATTTCCATCT CTGTCAACAT GGTATCAACA 
AGCGGAGCCT TTACGGCATA CAAGGACTTG ACCACTCCAA AAGCTATCGT CTTCACAGAG 
ACCGAGAGAC TTTACTACGA AGGAACTTTT GGAGGAGACA ACGACGAGCT TCTCGAGTTT 
ACGGGTCCGA TGCTGACTAC CTCGGCTCAA GTTGTCGTTT ACCCGTCCTT CAACGGAATC 
GACTACCTGG TCACCCCCGA TGATGCTGGT TTCACGATCA CCTACTACGC CCCTGCACCG 
GTCGCTCAGA GTCTTAAATT CTCCCCTACT GGACATCTTC TGGAACTCGA GTTCGACGTC 
GCAATCAACG AAGACTCGGT GACATCATGT GATGCAGTAT TCATGAATAT CACCAGCCTG 
GGTGGTGCTG CTGCCCAGTG CTTTGCATTC CCTTACCTAA AAACTATCTA TATACTACCC 
TTCAGTTCGG CCAGTGTAAA TCTTATTGGA CCAGATGATG AGCTAGTGCT GCGCGCTGAC 
GTTCTGAAAA AACGTGATGA GGAATACGCT CGGTACGCCA GCGGTACTCT TACCGCATCC 
TCCCCCGACG AGGCGGTAAC CGTCTACGCG GCTCTGACCG GTACAGAAGC AATTAGCAGC 
TGCGGTAATG TACGACTTAG TGCTCTGCAA TCCTTTGGAA ACGCAGGACG TTTTTTCACC 
TATCAGTGGT CTGTGACCAG TGGCGGTTCG ATCCCATCTG GCTTGACGGA TGATCTAACA 
GCCGCAGCGG AGTCCGCCTA TGTCACTCTC GATGGATCCT ACCTCGACGC GGACGTGCCG 
TATACATTCA GCATGACAGC AGAGAACTTC CTCGGAGGCA GTGACACAAA GACCTTGGAG 
GTGACTCGGT CTGCTCTCAA CAAACCAGAC GTCGTGATCT ACCCGGAAAG AGTTGATCCT 
AATAGTGCCC TTATCTCGCA AGCGTTCTAC CTCCGCGCTG CTGTACGATT CTACGAGGCC 
TGCGGTGGAG CTGAGCAGAT GGAGTATGCC TGGTCTGTAG ATGATGATAG TATTGCTCTT 
AACCTACAAA CCATAAACAA ACCACTCCTG TATGTGGCAG CTGGTTCTTT ACCAGGCGAC 
TCCCAAGTGA CCTTCACACT GGAGGTCAAC CGGGAAAGTG ATCCCAGCAC GGTGACCACG 
CAGACCGTGA CCATTACCAC GGTCTACACC GACCTCGACG CCCTAATCGA TGGCGGGAGT 
GAGCGGACCA TCGGCGTCGA CAGTGAGTCG TTCACACTGG ATGGATCGTC GTCGTTTGAT 
CCCGACAGTG AAAATGAAGA GATGACCTAC CTCTGGACTT GTCAGAAGTC GAACACTGAG 
ACAGGCAGCT ACGAGCCTTG TGTATCAAGC GAGACACGTG AGGTGTTCCC GGCGGAAGCT 
GACTCCAAAA CAAGTACTCT CACGTTAGCT TCCAACAATC TTTACTCGGA CTCCACCTAT 
CTGTTCACTC TGGAGATCAA CAAACTGTCA AGGACAGCAT CGACCAGTGT CACCATCCTT 
TCCAAGTCTG GCAATCCTCC AACGATCAGT CTCGATGAAT CCCTTCCTGA GAAGATAAAG 
AGCAGTAGTT CTCATACGCT ACGAGTATTG ATCAGCCATA CATCAGATAT TACCATTGAC 
TGGGCTACTG TCGACATAGA TCTTGGATAT GGATACGTTG ACTTTTCACA GCTGAATAGT 
CAGTCATTCC TTATACAAGG AGATACATCA CCCTTCATCA CATACGCCTT TGTAACTATC 
CCCACAGGAA CTTTGGAAGA GGATGTTGCC TACTCTTTCT CAGTGACGGT AACTGATACC 
GAAGGACAGG TGTCAGCTAG TAAATTGACT ACCAACACGT ATGGAGGAGT ATCATCCTGT 
ACATTCAGTT TGGGTGATGG TTCAACCAGC TATCAAGAAC TTGACGAGAT CACTCTCCTA 
ACTCAGAACT GCGTGACTGA TGAAGAAGCT TACCCTCTGT CTTACCAAGT GTTCCGTGAG 
AAGGTCGAAT CATCTGTCAC CCGTTACAAC GCGATGACGG GTGCATTGGC AGAAGCGAGC 
AGCACTATAT TGGGTCTGCC GTCCTTTAGC GACAACCAGA ACACATTTGT TGTCAAGGTG 
TGCAATGATT TTGGAAGCTG TTCCTCGTAC AGTCTTACAT TGGACGTCAC TCCCATCGCA 
TCTTTCAGTC AAGAACAGTT TGATGAAAGT ATAACCAATC TGGTAGAACC ACAAAAGCTC 
TCAGGGAATT TCCTAGATGC GCTGGTAAAC TTCAACACCA TCACGTCAGT GGGTGTCGAC 
AGCACGAGTA GGAAACGGAG AGCAGTCTCG GAGACATCCA GCACAGCTAC TGAGCAGCTG 
GATTTACTTC AGAATGCCAT CTCGTCGACG GTCCTTGACA CGGCCACCGC CCAGACTCTG 
CTTGATCAGT CGGATGGCGT GGTCATTGCT GATCTCACAC TGTCTGATAT GGACATATAC 
CTGGATATTT TAATTGAGCT CGTAGCTGTT TTCAGTGATG AAGGGCTTCC AGAGTCTAGC 
GCCGAGATCG TTCTTACAAA GACAAGCGAG ATCGCTGCTG AACTCGACCC TCAATACAAC 
TCGGACATGC TTGCCAAAAT TACGACCCTG AGAAACACAT TAATCCAGGG CCAGCTGAGT 
CAGATCCCAC TTGGAGGGGA CCCTACGGAG ACCAGGTCTG GTTCCGTTAC CACCAGGGCC 
TTCTATGCTA TCCCTAGTGA TACCCTCAGT ACTGCATCCA GCAACACCTC CTTCAACGTC 
AAGTTCGGAA GCGAGATCGA AAGCTTATAT GGTGCAAGTT GGTCGTGTGC TTCTGGAAGC 
ACTTGTTCTG GTGTTCAGAT CATGTTTACG CAGTACGACG ACGAGGTTGA TTACTACTCG 
ACTACGGACG AGGATAAGGC GAACAGAGCA TCTTCTATCC TTGAAATCGA TCTGCTTGAC 
CCGAGTGACA ATTCTGAGTT GAGTATTACT GGACTGAGCA GCCCCATATC AATCAACATG 
ACCGCTTCAC GCCTCCAATC CGACAAAGTC TATGACTGCT ATTGGTGGGA CACTAGCAGC 
AGCTCTTGGA GCCAGGATGG ACTTACGACC GTCTACCACT CCTCGGTCTT GTCTGAGTGC 
CAAACTACTC ATCTTTCAGC CTTCACCCTG ATCGCTGTTG ATTCTCCTAC TACGATTATG 
ATGGCTACCA CGATAGTTGA TGACGCCAGT ACTTCTACCG ATGCAGTAGC CTCGACTGCG 
ACAACGGAAA TCAGTGAGAG CGGCAGCAGT GGTCTAAGTT CTACCGTCAT CATCGTCATT 
GCCGTGGTCA TATCTGTCGT TTTGATCCTA ATTATTATCA TCGCCATCGT GGTCGTGATG 
TCGAAGAAGT CCTCCAAGGT CGGTAATGCT GATATCGAGA ACTCGAACGG CAATGGTGCC 
AGGAATGGGA CCAGCACCGC CGATGGACTC GCTCCTGTTG AATCTGCGAA CCAGGCATTC 
AATGCCCCAC CAACAGCACC GGCCCACCCA ACCCATACTG TTCAAGTGGT CGCTGCCCCA 
GCTTCGGCAC CTGCTTCTCA AGAGCCTATC ATGGACGTTG CAGACCTTCC TGGATCCCCA 
TCACCCCCTC CTGATGGACC CCATATTGAT ACCATCATAA AGGATGTTCA GAATGATAGT 
AGGGCCCAAA CTCCAATGGA TAGAGCCATG ACGCCCGTTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.
Genome research22(10)2079-87(2012 Oct)
Tu Q,Cameron RA,Worley KC,Gibbs RA,Davidson EH

The 10 sea urchin receptor for egg jelly proteins (SpREJ) are members of the polycystic kidney disease-1 (PKD1) family.
BMC genomics8235(2007 Jul)
Gunaratne HJ,Moy GW,Kinukawa M,Miyata S,Mah SA,Vacquier VD

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REJ8 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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