LOC8067274 cDNA ORF clone, Sorghum bicolor(sorghum)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8067274
Entrez Gene ID	8067274
Full Name	putative disease resistance protein RGA3
Gene Type	protein-coding
Organism	Sorghum bicolor(sorghum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021452917.1	XP_021308592.1	putative disease resistance protein RGA3 isoform X2
XM_021452919.1	XP_021308594.1	putative disease resistance protein RGA3 isoform X4
XM_002460000.1	XP_002460045.1	hypothetical protein
XM_021452916.1	XP_021308591.1	putative disease resistance protein RGA3 isoform X1
XM_021452920.1	XP_021308595.1	putative disease resistance protein RGA3 isoform X5
XM_021452918.1	XP_021308593.1	putative disease resistance protein RGA3 isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Sorghum bicolor genome and the diversification of grasses.
Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang H, Wang X, Wicker T, Bharti AK, Chapman J, Feltus FA, Gowik U, Grigoriev IV, Lyons E, Maher CA, Martis M, Narechania A, Otillar RP, Penning BW, Salamov AA, Wang Y, Zhang L, Carpita NC, Freeling M, Gingle AR, Hash CT, Keller B, Klein P, Kresovich S, McCann MC, Ming R, Peterson DG, Mehboob-ur-Rahman, Ware D, Westhoff P, Mayer KF, Messing J, Rokhsar DS
Nature457(7229)551-6(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC8067274 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8067274 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSc09753	XM_002460000.1 Latest version!	Sorghum bicolor hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OSc112595	XM_021452916.1 Latest version!	Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSc112596	XM_021452917.1 Latest version!	Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSc112597	XM_021452918.1 Latest version!	Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSc112598	XM_021452919.1 Latest version!	Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSc112599	XM_021452920.1 Latest version!	Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8067274 ( XM_002460000.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_002460045.1 Sorghum bicolor hypothetical protein, mRNA.

Price & Availability↑

CloneID	OSc09753
Clone ID Related Accession (Same CDS sequence)	XM_002460000.1
Accession Version	XM_002460000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1247328000000
Organism	Sorghum bicolor(Sorghum)
Product	hypothetical protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012871). COMPLETENESS: incomplete on both ends.

ATGGACATGG TAAGAGTTTT TTCTTACAAT GAGCTGCAAA CAGCCACACA TAAGTTTTCT 
GAGGCAAATA AGATTGGTGA AGGTTCTATT GGTTCTGTTT TCAGGGTAAT TTGCATAATT 
GATCACTTCA ATGGAGTTCT GGGAAGGCTT AAAGATGGAA TGGCTGTCGC AGTAAAGTTG 
CTCCATGATT CAAAGCAGGG CAGGCGAGAG TTTTTAGCTG AACTCACAGC AATTTCTGGC 
ATTGTGCATG AAAACCTAAT CACCTTAGTT GGATGCTGTT GTGAAAGGTC CCATAGAATC 
CTTGTATACA ATTATCTTGA GAACAAAAGC CTTGCAGACA AACTGTTAGG ATCAAGCAGT 
AGCACCATCC AACTCAGCTG GAGAGCTCGT GTAAAAATTG CTATAGGTGT TGCTCGTGGG 
CTTGCTTTTC TTCACGAAGA AATCCGCCCC TCCATAATTC ACCGGGACAT AAAAGCGAGC 
CATATTCTTC TTGACAAGGA TTTCACCCCA AAAGTTTCTG GTTTTGGGTT AACAAGGCTC 
CTCCCACCAA TTACAACTTC TATGGCCACT CGAGTTGCAG GAACAATAGG ATACATGGCT 
CCTGAATATG CAATCAGGGG ACAAGTGACA AAGAAGGCGG ATATCTTTAG TTTTGGTATT 
GTGCTACTGG AGATTGTTAG TGGCAAATGT AATCACGATA GTAGATTACC CCAGGAAGAC 
CAATTTCTGC TTAAAAGGTT ACCTTTCCAA TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002460045.1
CDS	1..756
Translation	MDMVRVFSYNELQTATHKFSEANKIGEGSIGSVFRVICIIDHFNGVLGRLKDGMAVAVKLLHDSKQGRREFLAELTAISGIVHENLITLVGCCCERSHRILVYNYLENKSLADKLLGSSSSTIQLSWRARVKIAIGVARGLAFLHEEIRPSIIHRDIKASHILLDKDFTPKVSGFGLTRLLPPITTSMATRVAGTIGYMAPEYAIRGQVTKKADIFSFGIVLLEIVSGKCNHDSRLPQEDQFLLKRLPFQS

Target ORF information:

RefSeq Version	XM_002460000.1
Organism	Sorghum bicolor(Sorghum)
Definition	Sorghum bicolor hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002460000.1

ORF Insert Sequence:

ATGGACATGG TAAGAGTTTT TTCTTACAAT GAGCTGCAAA CAGCCACACA TAAGTTTTCT 
GAGGCAAATA AGATTGGTGA AGGTTCTATT GGTTCTGTTT TCAGGGTAAT TTGCATAATT 
GATCACTTCA ATGGAGTTCT GGGAAGGCTT AAAGATGGAA TGGCTGTCGC AGTAAAGTTG 
CTCCATGATT CAAAGCAGGG CAGGCGAGAG TTTTTAGCTG AACTCACAGC AATTTCTGGC 
ATTGTGCATG AAAACCTAAT CACCTTAGTT GGATGCTGTT GTGAAAGGTC CCATAGAATC 
CTTGTATACA ATTATCTTGA GAACAAAAGC CTTGCAGACA AACTGTTAGG ATCAAGCAGT 
AGCACCATCC AACTCAGCTG GAGAGCTCGT GTAAAAATTG CTATAGGTGT TGCTCGTGGG 
CTTGCTTTTC TTCACGAAGA AATCCGCCCC TCCATAATTC ACCGGGACAT AAAAGCGAGC 
CATATTCTTC TTGACAAGGA TTTCACCCCA AAAGTTTCTG GTTTTGGGTT AACAAGGCTC 
CTCCCACCAA TTACAACTTC TATGGCCACT CGAGTTGCAG GAACAATAGG ATACATGGCT 
CCTGAATATG CAATCAGGGG ACAAGTGACA AAGAAGGCGG ATATCTTTAG TTTTGGTATT 
GTGCTACTGG AGATTGTTAG TGGCAAATGT AATCACGATA GTAGATTACC CCAGGAAGAC 
CAATTTCTGC TTAAAAGGTT ACCTTTCCAA TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8067274 ( XM_021452916.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_021308591.1 Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSc112595
Clone ID Related Accession (Same CDS sequence)	XM_021452916.1
Accession Version	XM_021452916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	putative disease resistance protein RGA3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012871.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG TTAGCGCTGA TATAGATCGA CTCCAAAGGA GGCTTGCCTC CGACGGCCAA 
CGAATCAAGC TTCCCTCCAG CATACGGACG GATCTATCGC GCATGACAAG AGCTCTCTAC 
AGGTTGCAAG GCATCCTGAC TAATGTGGAG AAGCAACCAT TTGACGGGAG CAGAGAACCC 
TGCTTGAGCA AGATCAAACA GAATATTTAC GATGTGGAAG ATATACTAGA CGAGCTTGAA 
AATGGAAGCT TCAGGGCCCA ACGGAGCAGC AGGGGTTGGA AGCTAGCCGA GGCTTCAGAT 
CTCTGGTCAC AGGTAGCATT TTCCTTCTCA TCTAACCACT CAGTTCTGCA TTGTCGTATG 
ATGAATAAAA TGAAGAAGAT CAGAAAAAGT TTGGATCATG CATCAGAAGA CTCCTTCATA 
TTCACCTTAC TGCACCACAG AGCAGATGCA GATCAATCAG ATCACAATGT GTTTGATGAA 
AATGCAATCA TTGGAAGAAA TAAGGATAAG GAAAATGTAA GAGTCTTGCT CTTGAGTAAC 
AGTGAACAAA AAATTTCTAT TATTCCTATA GTTGGTCTTG GGGGATTGGG GAAAACAGCT 
CTTGCACAAC TCATTTTCAG TGACCAAGAT GAACGATATA ATTTTGACCT TCGTGTATGG 
ATCAATCTGA ACATGAGTTA TGATTTTAAC AGTATTGCTA GTGCTATAAT CTCAGAAGTT 
AACATAACAG AGGAAGGTAC CTCACAAGTT AATGGGGGTG AATATAATCC TCAGATACTA 
ATGAATTGTC TTCTAGAAGT ACTTAATGAT AAAAGATGTC TGATTGTGTT GGATGGCCTA 
TGGAGCATAG ATGAAGGGCA ATTACTTCAT CTCAAGAGAA TGCTACAGAG CACACAGAAT 
ACCAAGATTA TAGTGACTAC TTGTAGTGAA AATGTTGCAA ATCTGATGCA TACTGTTCGT 
CCCTACAAAT TGGATCCTTT ATCTGAAGAA AATTGTTGGA CAATATTTTC TCAAAGGGTA 
TTTGGTGGTG GAGATAACTC AGACCTCACA CGAATTGGGA AGCAGATCGT GAACAAGTGT 
GAAGGTATAC CGGGAGTTAT TCATTCTCTT GGTGCATTCA TGCACGATAA CGGAACGAGC 
ACTTGGAATT GGAATGACAA AAATGAAGAG CTGTGGAAAT TGGAGAAACA GTTTCCATTG 
CAGATCAACT TGTTCTCATC AGTCAAACAG ATATATTACA AGATGCCATT GGCATTGAAA 
TCGTGCCTCA GCTATTTATC GATGTTTCCT AAAGGATCCA ATATTCGTAT GGAAAATGTT 
ATTACACAGT GGGCTGCACT TGGCATCCTT GGATCAACAC ATGGATCATT GCCTGTTTAT 
GCCCAGGGGA GGAAGTATAT CCAGGAACTG CTTTCTGTGT TCTTCCTTGA AGCACATGAT 
AAATCCACAG ATTTTGGAAT TAATCATGCT AGTGCCTCAA AAGTCTTTAA AATGCACAGT 
TTAGTTCATG AGTTTGCCAG ATATGTTACC AGTCATGATT TATTTATTCT GGATGGTGAA 
AGGATGCGTA ACGATAACCC AGGGAATCGT ACTTCTGGAT ATGCACTACT GACTAGGTGC 
AGTGATAAAT CCAAAGTTAG CAGAGGTTTC CTGAAAAGTG TAAGGGCAAT ATGTCTGAAG 
GACTGCAGGG GAACAAAGCT TATTGAGAAA ATATTCTCTG CTTTAAAGCA TCTACGTGTG 
TTGGACCTTA GTAGATGCTC ATTTCTGGAG TTACCTAGTT CTATTTGCCA GTTAACCCAT 
CTAAGGTACA TTGATATTTC TTGTTCGGCT ATTCAATCAC TACCTGATCA AATGAGTTCT 
GTACAACACC TTGAAGCACT GGATCTCTCG GGGACATGTA TTCAAGTCCT ACCTGATTTT 
GTTAGAACAT TTAAGAAGCT AACATATCTT AACCTACAAG AATGCTGGGA ACTCCGTCAC 
TTGCCCTCAA AGCTTGATGA TATTAAGAGT TTGCAGCATC TTAACCTGTC ATGCTGTCCT 
GCAGCTCACC AGCTAGTTGA ATCAATCAGT GGATTTCAAG AACTTCGGTT TTTGGATATA 
TCAAGTTGCA CAGAACTTCA AACATTGCCA GAATCCTTCG TCAGACTAAC AAATTTAGAG 
GACCTAATCC TGTCCAAATG CACCAGGCTC AAGAAGCTAC CAGAATCATT TGGTGATAAA 
CTCTGTTTTC TTCGGTTCTT GAACATATCA TATTGCTGTG AGCTAGAAGA AGTGCCTGCA 
TCTCTCGGAA GGCTTGCTAG TTTGGAGGTT CTTATACTTT CAGGGTGCAA CAGAATTCAG 
AATCTCCCTC AATCATTCAG TGACATTGCA TTCTTACGGA TGCTAGACCT TTCAGGGTGT 
GCAGATCTTC ACATGGACCT TGGAATGCTA CCAAATAATA ATTTGGAGAA TCTTAATGTG 
GATGGATGTT GTAAGGTCTA TGCTATGCCT GGGTGGACTG TGAACTTCCC TAAACTACTT 
CCTGAATGTC TACAAACTTG TGACCAACAT GTTCGACGCT TAATTGAACA CAAGCAAGCA 
TGTCCCAATC ATAGTGAATT AGAGATCAGC AATGAACTAA ATGTGCTTGA CGAAACTGGG 
GAAGCAATGG CAAGTCAATC ATTTCAACAT GCTGGTGACT CTGAATGTAC CACCAAGAAA 
GTCATTGAGG ACTCATCTGC GCATACAAGT CCTAAAATTC CTCTGCATGG CACTACCCCT 
ACTGGAGTCC AGCTGAAATC AGCTGGTTCG TTTTTTGGGG CTTCTACAAT TCGATTTTCG 
AAAATGTCTC GTTGTTTTAT GTGTACAACA GACTTGAAAA TGAATAGTCA AGGTGAAGGT 
GAAGGAGAAC ACATGGACAT GGTAAGAGTT TTTTCTTACA ATGAGCTGCA AACAGCCACA 
CATAAGTTTT CTGAGGCAAA TAAGATTGGT GAAGGTTCTA TTGGTTCTGT TTTCAGGGGA 
AGGCTTAAAG ATGGAATGGC TGTCGCAGTA AAGTTGCTCC ATGATTCAAA GCAGGGCAGG 
CGAGAGTTTT TAGCTGAACT CACAGCAATT TCTGGCATTG TGCATGAAAA CCTAATCACC 
TTAGTTGGAT GCTGTTGTGA AAGGTCCCAT AGAATCCTTG TATACAATTA TCTTGAGAAC 
AAAAGCCTTG CAGACAAACT GTTAGGATCA AGCAGTAGCA CCATCCAACT CAGCTGGAGA 
GCTCGTGTAA AAATTGCTAT AGGTGTTGCT CGTGGGCTTG CTTTTCTTCA CGAAGAAATC 
CGCCCCTCCA TAATTCACCG GGACATAAAA GCGAGCCATA TTCTTCTTGA CAAGGATTTC 
ACCCCAAAAG TTTCTGGTTT TGGGTTAACA AGGCTCCTCC CACCAATTAC AACTTCTATG 
GCCACTCGAG TTGCAGGAAC AATAGGATAC ATGGCTCCTG AATATGCAAT CAGGGGACAA 
GTGACAAAGA AGGCGGATAT CTTTAGTTTT GGTATTGTGC TACTGGAGAT TGTTAGTGGC 
AAATGTAATC ACGATAGTAG ATTACCCCAG GAAGACCAAT TTCTGCTTAA AAGGACGTGG 
AGATCCTCTG AGGAAGGAAA TCTGGTGGAA ATCATAGATA AAGATTTAGG GGACGACCTC 
GATGTTGAGG AGGCATGCCG ATTCTTGAAT GTTGGTCTAC TATGTACTCA AAACGAGATG 
GCGAGGCGTC CCAACATGAG GAACATCGTA AAGATGCTGA CTGGGGAGAA AGGAGTCTCC 
GTGGTCAAGG TGACAAGACC AATAATGCTG TGGTCTGACT TGCTGGAATT AAAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021308591.1
CDS	193..4032
Translation	MATVSADIDRLQRRLASDGQRIKLPSSIRTDLSRMTRALYRLQGILTNVEKQPFDGSREPCLSKIKQNIYDVEDILDELENGSFRAQRSSRGWKLAEASDLWSQVAFSFSSNHSVLHCRMMNKMKKIRKSLDHASEDSFIFTLLHHRADADQSDHNVFDENAIIGRNKDKENVRVLLLSNSEQKISIIPIVGLGGLGKTALAQLIFSDQDERYNFDLRVWINLNMSYDFNSIASAIISEVNITEEGTSQVNGGEYNPQILMNCLLEVLNDKRCLIVLDGLWSIDEGQLLHLKRMLQSTQNTKIIVTTCSENVANLMHTVRPYKLDPLSEENCWTIFSQRVFGGGDNSDLTRIGKQIVNKCEGIPGVIHSLGAFMHDNGTSTWNWNDKNEELWKLEKQFPLQINLFSSVKQIYYKMPLALKSCLSYLSMFPKGSNIRMENVITQWAALGILGSTHGSLPVYAQGRKYIQELLSVFFLEAHDKSTDFGINHASASKVFKMHSLVHEFARYVTSHDLFILDGERMRNDNPGNRTSGYALLTRCSDKSKVSRGFLKSVRAICLKDCRGTKLIEKIFSALKHLRVLDLSRCSFLELPSSICQLTHLRYIDISCSAIQSLPDQMSSVQHLEALDLSGTCIQVLPDFVRTFKKLTYLNLQECWELRHLPSKLDDIKSLQHLNLSCCPAAHQLVESISGFQELRFLDISSCTELQTLPESFVRLTNLEDLILSKCTRLKKLPESFGDKLCFLRFLNISYCCELEEVPASLGRLASLEVLILSGCNRIQNLPQSFSDIAFLRMLDLSGCADLHMDLGMLPNNNLENLNVDGCCKVYAMPGWTVNFPKLLPECLQTCDQHVRRLIEHKQACPNHSELEISNELNVLDETGEAMASQSFQHAGDSECTTKKVIEDSSAHTSPKIPLHGTTPTGVQLKSAGSFFGASTIRFSKMSRCFMCTTDLKMNSQGEGEGEHMDMVRVFSYNELQTATHKFSEANKIGEGSIGSVFRGRLKDGMAVAVKLLHDSKQGRREFLAELTAISGIVHENLITLVGCCCERSHRILVYNYLENKSLADKLLGSSSSTIQLSWRARVKIAIGVARGLAFLHEEIRPSIIHRDIKASHILLDKDFTPKVSGFGLTRLLPPITTSMATRVAGTIGYMAPEYAIRGQVTKKADIFSFGIVLLEIVSGKCNHDSRLPQEDQFLLKRTWRSSEEGNLVEIIDKDLGDDLDVEEACRFLNVGLLCTQNEMARRPNMRNIVKMLTGEKGVSVVKVTRPIMLWSDLLELKT

Target ORF information:

RefSeq Version	XM_021452916.1
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021452916.1

ORF Insert Sequence:

ATGGCCACTG TTAGCGCTGA TATAGATCGA CTCCAAAGGA GGCTTGCCTC CGACGGCCAA 
CGAATCAAGC TTCCCTCCAG CATACGGACG GATCTATCGC GCATGACAAG AGCTCTCTAC 
AGGTTGCAAG GCATCCTGAC TAATGTGGAG AAGCAACCAT TTGACGGGAG CAGAGAACCC 
TGCTTGAGCA AGATCAAACA GAATATTTAC GATGTGGAAG ATATACTAGA CGAGCTTGAA 
AATGGAAGCT TCAGGGCCCA ACGGAGCAGC AGGGGTTGGA AGCTAGCCGA GGCTTCAGAT 
CTCTGGTCAC AGGTAGCATT TTCCTTCTCA TCTAACCACT CAGTTCTGCA TTGTCGTATG 
ATGAATAAAA TGAAGAAGAT CAGAAAAAGT TTGGATCATG CATCAGAAGA CTCCTTCATA 
TTCACCTTAC TGCACCACAG AGCAGATGCA GATCAATCAG ATCACAATGT GTTTGATGAA 
AATGCAATCA TTGGAAGAAA TAAGGATAAG GAAAATGTAA GAGTCTTGCT CTTGAGTAAC 
AGTGAACAAA AAATTTCTAT TATTCCTATA GTTGGTCTTG GGGGATTGGG GAAAACAGCT 
CTTGCACAAC TCATTTTCAG TGACCAAGAT GAACGATATA ATTTTGACCT TCGTGTATGG 
ATCAATCTGA ACATGAGTTA TGATTTTAAC AGTATTGCTA GTGCTATAAT CTCAGAAGTT 
AACATAACAG AGGAAGGTAC CTCACAAGTT AATGGGGGTG AATATAATCC TCAGATACTA 
ATGAATTGTC TTCTAGAAGT ACTTAATGAT AAAAGATGTC TGATTGTGTT GGATGGCCTA 
TGGAGCATAG ATGAAGGGCA ATTACTTCAT CTCAAGAGAA TGCTACAGAG CACACAGAAT 
ACCAAGATTA TAGTGACTAC TTGTAGTGAA AATGTTGCAA ATCTGATGCA TACTGTTCGT 
CCCTACAAAT TGGATCCTTT ATCTGAAGAA AATTGTTGGA CAATATTTTC TCAAAGGGTA 
TTTGGTGGTG GAGATAACTC AGACCTCACA CGAATTGGGA AGCAGATCGT GAACAAGTGT 
GAAGGTATAC CGGGAGTTAT TCATTCTCTT GGTGCATTCA TGCACGATAA CGGAACGAGC 
ACTTGGAATT GGAATGACAA AAATGAAGAG CTGTGGAAAT TGGAGAAACA GTTTCCATTG 
CAGATCAACT TGTTCTCATC AGTCAAACAG ATATATTACA AGATGCCATT GGCATTGAAA 
TCGTGCCTCA GCTATTTATC GATGTTTCCT AAAGGATCCA ATATTCGTAT GGAAAATGTT 
ATTACACAGT GGGCTGCACT TGGCATCCTT GGATCAACAC ATGGATCATT GCCTGTTTAT 
GCCCAGGGGA GGAAGTATAT CCAGGAACTG CTTTCTGTGT TCTTCCTTGA AGCACATGAT 
AAATCCACAG ATTTTGGAAT TAATCATGCT AGTGCCTCAA AAGTCTTTAA AATGCACAGT 
TTAGTTCATG AGTTTGCCAG ATATGTTACC AGTCATGATT TATTTATTCT GGATGGTGAA 
AGGATGCGTA ACGATAACCC AGGGAATCGT ACTTCTGGAT ATGCACTACT GACTAGGTGC 
AGTGATAAAT CCAAAGTTAG CAGAGGTTTC CTGAAAAGTG TAAGGGCAAT ATGTCTGAAG 
GACTGCAGGG GAACAAAGCT TATTGAGAAA ATATTCTCTG CTTTAAAGCA TCTACGTGTG 
TTGGACCTTA GTAGATGCTC ATTTCTGGAG TTACCTAGTT CTATTTGCCA GTTAACCCAT 
CTAAGGTACA TTGATATTTC TTGTTCGGCT ATTCAATCAC TACCTGATCA AATGAGTTCT 
GTACAACACC TTGAAGCACT GGATCTCTCG GGGACATGTA TTCAAGTCCT ACCTGATTTT 
GTTAGAACAT TTAAGAAGCT AACATATCTT AACCTACAAG AATGCTGGGA ACTCCGTCAC 
TTGCCCTCAA AGCTTGATGA TATTAAGAGT TTGCAGCATC TTAACCTGTC ATGCTGTCCT 
GCAGCTCACC AGCTAGTTGA ATCAATCAGT GGATTTCAAG AACTTCGGTT TTTGGATATA 
TCAAGTTGCA CAGAACTTCA AACATTGCCA GAATCCTTCG TCAGACTAAC AAATTTAGAG 
GACCTAATCC TGTCCAAATG CACCAGGCTC AAGAAGCTAC CAGAATCATT TGGTGATAAA 
CTCTGTTTTC TTCGGTTCTT GAACATATCA TATTGCTGTG AGCTAGAAGA AGTGCCTGCA 
TCTCTCGGAA GGCTTGCTAG TTTGGAGGTT CTTATACTTT CAGGGTGCAA CAGAATTCAG 
AATCTCCCTC AATCATTCAG TGACATTGCA TTCTTACGGA TGCTAGACCT TTCAGGGTGT 
GCAGATCTTC ACATGGACCT TGGAATGCTA CCAAATAATA ATTTGGAGAA TCTTAATGTG 
GATGGATGTT GTAAGGTCTA TGCTATGCCT GGGTGGACTG TGAACTTCCC TAAACTACTT 
CCTGAATGTC TACAAACTTG TGACCAACAT GTTCGACGCT TAATTGAACA CAAGCAAGCA 
TGTCCCAATC ATAGTGAATT AGAGATCAGC AATGAACTAA ATGTGCTTGA CGAAACTGGG 
GAAGCAATGG CAAGTCAATC ATTTCAACAT GCTGGTGACT CTGAATGTAC CACCAAGAAA 
GTCATTGAGG ACTCATCTGC GCATACAAGT CCTAAAATTC CTCTGCATGG CACTACCCCT 
ACTGGAGTCC AGCTGAAATC AGCTGGTTCG TTTTTTGGGG CTTCTACAAT TCGATTTTCG 
AAAATGTCTC GTTGTTTTAT GTGTACAACA GACTTGAAAA TGAATAGTCA AGGTGAAGGT 
GAAGGAGAAC ACATGGACAT GGTAAGAGTT TTTTCTTACA ATGAGCTGCA AACAGCCACA 
CATAAGTTTT CTGAGGCAAA TAAGATTGGT GAAGGTTCTA TTGGTTCTGT TTTCAGGGGA 
AGGCTTAAAG ATGGAATGGC TGTCGCAGTA AAGTTGCTCC ATGATTCAAA GCAGGGCAGG 
CGAGAGTTTT TAGCTGAACT CACAGCAATT TCTGGCATTG TGCATGAAAA CCTAATCACC 
TTAGTTGGAT GCTGTTGTGA AAGGTCCCAT AGAATCCTTG TATACAATTA TCTTGAGAAC 
AAAAGCCTTG CAGACAAACT GTTAGGATCA AGCAGTAGCA CCATCCAACT CAGCTGGAGA 
GCTCGTGTAA AAATTGCTAT AGGTGTTGCT CGTGGGCTTG CTTTTCTTCA CGAAGAAATC 
CGCCCCTCCA TAATTCACCG GGACATAAAA GCGAGCCATA TTCTTCTTGA CAAGGATTTC 
ACCCCAAAAG TTTCTGGTTT TGGGTTAACA AGGCTCCTCC CACCAATTAC AACTTCTATG 
GCCACTCGAG TTGCAGGAAC AATAGGATAC ATGGCTCCTG AATATGCAAT CAGGGGACAA 
GTGACAAAGA AGGCGGATAT CTTTAGTTTT GGTATTGTGC TACTGGAGAT TGTTAGTGGC 
AAATGTAATC ACGATAGTAG ATTACCCCAG GAAGACCAAT TTCTGCTTAA AAGGACGTGG 
AGATCCTCTG AGGAAGGAAA TCTGGTGGAA ATCATAGATA AAGATTTAGG GGACGACCTC 
GATGTTGAGG AGGCATGCCG ATTCTTGAAT GTTGGTCTAC TATGTACTCA AAACGAGATG 
GCGAGGCGTC CCAACATGAG GAACATCGTA AAGATGCTGA CTGGGGAGAA AGGAGTCTCC 
GTGGTCAAGG TGACAAGACC AATAATGCTG TGGTCTGACT TGCTGGAATT AAAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8067274 ( XM_021452917.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_021308592.1 Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSc112596
Clone ID Related Accession (Same CDS sequence)	XM_021452917.1
Accession Version	XM_021452917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	putative disease resistance protein RGA3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012871.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG TTAGCGCTGA TATAGATCGA CTCCAAAGGA GGCTTGCCTC CGACGGCCAA 
CGAATCAAGC TTCCCTCCAG CATACGGACG GATCTATCGC GCATGACAAG AGCTCTCTAC 
AGGTTGCAAG GCATCCTGAC TAATGTGGAG AAGCAACCAT TTGACGGGAG CAGAGAACCC 
TGCTTGAGCA AGATCAAACA GAATATTTAC GATGTGGAAG ATATACTAGA CGAGCTTGAA 
AATGGAAGCT TCAGGGCCCA ACGGAGCAGC AGGGGTTGGA AGCTAGCCGA GGCTTCAGAT 
CTCTGGTCAC AGGTAGCATT TTCCTTCTCA TCTAACCACT CAGTTCTGCA TTGTCGTATG 
ATGAATAAAA TGAAGAAGAT CAGAAAAAGT TTGGATCATG CATCAGAAGA CTCCTTCATA 
TTCACCTTAC TGCACCACAG AGCAGATGCA GATCAATCAG ATCACAATGT GTTTGATGAA 
AATGCAATCA TTGGAAGAAA TAAGGATAAG GAAAATGTAA GAGTCTTGCT CTTGAGTAAC 
AGTGAACAAA AAATTTCTAT TATTCCTATA GTTGGTCTTG GGGGATTGGG GAAAACAGCT 
CTTGCACAAC TCATTTTCAG TGACCAAGAT GAACGATATA ATTTTGACCT TCGTGTATGG 
ATCAATCTGA ACATGAGTTA TGATTTTAAC AGTATTGCTA GTGCTATAAT CTCAGAAGTT 
AACATAACAG AGGAAGGTAC CTCACAAGTT AATGGGGGTG AATATAATCC TCAGATACTA 
ATGAATTGTC TTCTAGAAGT ACTTAATGAT AAAAGATGTC TGATTGTGTT GGATGGCCTA 
TGGAGCATAG ATGAAGGGCA ATTACTTCAT CTCAAGAGAA TGCTACAGAG CACACAGAAT 
ACCAAGATTA TAGTGACTAC TTGTAGTGAA AATGTTGCAA ATCTGATGCA TACTGTTCGT 
CCCTACAAAT TGGATCCTTT ATCTGAAGAA AATTGTTGGA CAATATTTTC TCAAAGGGTA 
TTTGGTGGTG GAGATAACTC AGACCTCACA CGAATTGGGA AGCAGATCGT GAACAAGTGT 
GAAGGTATAC CGGGAGTTAT TCATTCTCTT GGTGCATTCA TGCACGATAA CGGAACGAGC 
ACTTGGAATT GGAATGACAA AAATGAAGAG CTGTGGAAAT TGGAGAAACA GTTTCCATTG 
CAGATCAACT TGTTCTCATC AGTCAAACAG ATATATTACA AGATGCCATT GGCATTGAAA 
TCGTGCCTCA GCTATTTATC GATGTTTCCT AAAGGATCCA ATATTCGTAT GGAAAATGTT 
ATTACACAGT GGGCTGCACT TGGCATCCTT GGATCAACAC ATGGATCATT GCCTGTTTAT 
GCCCAGGGGA GGAAGTATAT CCAGGAACTG CTTTCTGTGT TCTTCCTTGA AGCACATGAT 
AAATCCACAG ATTTTGGAAT TAATCATGCT AGTGCCTCAA AAGTCTTTAA AATGCACAGT 
TTAGTTCATG AGTTTGCCAG ATATGTTACC AGTCATGATT TATTTATTCT GGATGGTGAA 
AGGATGCGTA ACGATAACCC AGGGAATCGT ACTTCTGGAT ATGCACTACT GACTAGGTGC 
AGTGATAAAT CCAAAGTTAG CAGAGGTTTC CTGAAAAGTG TAAGGGCAAT ATGTCTGAAG 
GACTGCAGGG GAACAAAGCT TATTGAGAAA ATATTCTCTG CTTTAAAGCA TCTACGTGTG 
TTGGACCTTA GTAGATGCTC ATTTCTGGAG TTACCTAGTT CTATTTGCCA GTTAACCCAT 
CTAAGGTACA TTGATATTTC TTGTTCGGCT ATTCAATCAC TACCTGATCA AATGAGTTCT 
GTACAACACC TTGAAGCACT GGATCTCTCG GGGACATGTA TTCAAGTCCT ACCTGATTTT 
GTTAGAACAT TTAAGAAGCT AACATATCTT AACCTACAAG AATGCTGGGA ACTCCGTCAC 
TTGCCCTCAA AGCTTGATGA TATTAAGAGT TTGCAGCATC TTAACCTGTC ATGCTGTCCT 
GCAGCTCACC AGCTAGTTGA ATCAATCAGT GGATTTCAAG AACTTCGGTT TTTGGATATA 
TCAAGTTGCA CAGAACTTCA AACATTGCCA GAATCCTTCG TCAGACTAAC AAATTTAGAG 
GACCTAATCC TGTCCAAATG CACCAGGCTC AAGAAGCTAC CAGAATCATT TGGTGATAAA 
CTCTGTTTTC TTCGGTTCTT GAACATATCA TATTGCTGTG AGCTAGAAGA AGTGCCTGCA 
TCTCTCGGAA GGCTTGCTAG TTTGGAGGTT CTTATACTTT CAGGGTGCAA CAGAATTCAG 
AATCTCCCTC AATCATTCAG TGACATTGCA TTCTTACGGA TGCTAGACCT TTCAGGGTGT 
GCAGATCTTC ACATGGACCT TGGAATGCTA CCAAATAATA ATTTGGAGAA TCTTAATGTG 
GATGGATGTT GTAAGGTCTA TGCTATGCCT GGGTGGACTG TGAACTTCCC TAAACTACTT 
CCTGAATGTC TACAAACTTG TGACCAACAT GTTCGACGCT TAATTGAACA CAAGCAAGCA 
TGTCCCAATC ATAGTGAATT AGAGATCAGC AATGAACTAA ATGTGCTTGA CGAAACTGGG 
GAAGCAATGG CAAGTCAATC ATTTCAACAT GCTGGTGACT CTGAATGTAC CACCAAGAAA 
GTCATTGAGG ACTCATCTGC GCATACAAGT CCTAAAATTC CTCTGCATGG CACTACCCCT 
ACTGGAGTCC AGCTGAAATC AGCTGGTTCG TTTTTTGGGG CTTCTACAAT TCGATTTTCG 
AAAATGTCTC GTTGTTTTAT GTGTACAACA GACTTGAAAA TGAATAGTCA AGGTGAAGGA 
GAACACATGG ACATGGTAAG AGTTTTTTCT TACAATGAGC TGCAAACAGC CACACATAAG 
TTTTCTGAGG CAAATAAGAT TGGTGAAGGT TCTATTGGTT CTGTTTTCAG GGGAAGGCTT 
AAAGATGGAA TGGCTGTCGC AGTAAAGTTG CTCCATGATT CAAAGCAGGG CAGGCGAGAG 
TTTTTAGCTG AACTCACAGC AATTTCTGGC ATTGTGCATG AAAACCTAAT CACCTTAGTT 
GGATGCTGTT GTGAAAGGTC CCATAGAATC CTTGTATACA ATTATCTTGA GAACAAAAGC 
CTTGCAGACA AACTGTTAGG ATCAAGCAGT AGCACCATCC AACTCAGCTG GAGAGCTCGT 
GTAAAAATTG CTATAGGTGT TGCTCGTGGG CTTGCTTTTC TTCACGAAGA AATCCGCCCC 
TCCATAATTC ACCGGGACAT AAAAGCGAGC CATATTCTTC TTGACAAGGA TTTCACCCCA 
AAAGTTTCTG GTTTTGGGTT AACAAGGCTC CTCCCACCAA TTACAACTTC TATGGCCACT 
CGAGTTGCAG GAACAATAGG ATACATGGCT CCTGAATATG CAATCAGGGG ACAAGTGACA 
AAGAAGGCGG ATATCTTTAG TTTTGGTATT GTGCTACTGG AGATTGTTAG TGGCAAATGT 
AATCACGATA GTAGATTACC CCAGGAAGAC CAATTTCTGC TTAAAAGGAC GTGGAGATCC 
TCTGAGGAAG GAAATCTGGT GGAAATCATA GATAAAGATT TAGGGGACGA CCTCGATGTT 
GAGGAGGCAT GCCGATTCTT GAATGTTGGT CTACTATGTA CTCAAAACGA GATGGCGAGG 
CGTCCCAACA TGAGGAACAT CGTAAAGATG CTGACTGGGG AGAAAGGAGT CTCCGTGGTC 
AAGGTGACAA GACCAATAAT GCTGTGGTCT GACTTGCTGG AATTAAAAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021308592.1
CDS	193..4026
Translation	MATVSADIDRLQRRLASDGQRIKLPSSIRTDLSRMTRALYRLQGILTNVEKQPFDGSREPCLSKIKQNIYDVEDILDELENGSFRAQRSSRGWKLAEASDLWSQVAFSFSSNHSVLHCRMMNKMKKIRKSLDHASEDSFIFTLLHHRADADQSDHNVFDENAIIGRNKDKENVRVLLLSNSEQKISIIPIVGLGGLGKTALAQLIFSDQDERYNFDLRVWINLNMSYDFNSIASAIISEVNITEEGTSQVNGGEYNPQILMNCLLEVLNDKRCLIVLDGLWSIDEGQLLHLKRMLQSTQNTKIIVTTCSENVANLMHTVRPYKLDPLSEENCWTIFSQRVFGGGDNSDLTRIGKQIVNKCEGIPGVIHSLGAFMHDNGTSTWNWNDKNEELWKLEKQFPLQINLFSSVKQIYYKMPLALKSCLSYLSMFPKGSNIRMENVITQWAALGILGSTHGSLPVYAQGRKYIQELLSVFFLEAHDKSTDFGINHASASKVFKMHSLVHEFARYVTSHDLFILDGERMRNDNPGNRTSGYALLTRCSDKSKVSRGFLKSVRAICLKDCRGTKLIEKIFSALKHLRVLDLSRCSFLELPSSICQLTHLRYIDISCSAIQSLPDQMSSVQHLEALDLSGTCIQVLPDFVRTFKKLTYLNLQECWELRHLPSKLDDIKSLQHLNLSCCPAAHQLVESISGFQELRFLDISSCTELQTLPESFVRLTNLEDLILSKCTRLKKLPESFGDKLCFLRFLNISYCCELEEVPASLGRLASLEVLILSGCNRIQNLPQSFSDIAFLRMLDLSGCADLHMDLGMLPNNNLENLNVDGCCKVYAMPGWTVNFPKLLPECLQTCDQHVRRLIEHKQACPNHSELEISNELNVLDETGEAMASQSFQHAGDSECTTKKVIEDSSAHTSPKIPLHGTTPTGVQLKSAGSFFGASTIRFSKMSRCFMCTTDLKMNSQGEGEHMDMVRVFSYNELQTATHKFSEANKIGEGSIGSVFRGRLKDGMAVAVKLLHDSKQGRREFLAELTAISGIVHENLITLVGCCCERSHRILVYNYLENKSLADKLLGSSSSTIQLSWRARVKIAIGVARGLAFLHEEIRPSIIHRDIKASHILLDKDFTPKVSGFGLTRLLPPITTSMATRVAGTIGYMAPEYAIRGQVTKKADIFSFGIVLLEIVSGKCNHDSRLPQEDQFLLKRTWRSSEEGNLVEIIDKDLGDDLDVEEACRFLNVGLLCTQNEMARRPNMRNIVKMLTGEKGVSVVKVTRPIMLWSDLLELKT

Target ORF information:

RefSeq Version	XM_021452917.1
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021452917.1

ORF Insert Sequence:

ATGGCCACTG TTAGCGCTGA TATAGATCGA CTCCAAAGGA GGCTTGCCTC CGACGGCCAA 
CGAATCAAGC TTCCCTCCAG CATACGGACG GATCTATCGC GCATGACAAG AGCTCTCTAC 
AGGTTGCAAG GCATCCTGAC TAATGTGGAG AAGCAACCAT TTGACGGGAG CAGAGAACCC 
TGCTTGAGCA AGATCAAACA GAATATTTAC GATGTGGAAG ATATACTAGA CGAGCTTGAA 
AATGGAAGCT TCAGGGCCCA ACGGAGCAGC AGGGGTTGGA AGCTAGCCGA GGCTTCAGAT 
CTCTGGTCAC AGGTAGCATT TTCCTTCTCA TCTAACCACT CAGTTCTGCA TTGTCGTATG 
ATGAATAAAA TGAAGAAGAT CAGAAAAAGT TTGGATCATG CATCAGAAGA CTCCTTCATA 
TTCACCTTAC TGCACCACAG AGCAGATGCA GATCAATCAG ATCACAATGT GTTTGATGAA 
AATGCAATCA TTGGAAGAAA TAAGGATAAG GAAAATGTAA GAGTCTTGCT CTTGAGTAAC 
AGTGAACAAA AAATTTCTAT TATTCCTATA GTTGGTCTTG GGGGATTGGG GAAAACAGCT 
CTTGCACAAC TCATTTTCAG TGACCAAGAT GAACGATATA ATTTTGACCT TCGTGTATGG 
ATCAATCTGA ACATGAGTTA TGATTTTAAC AGTATTGCTA GTGCTATAAT CTCAGAAGTT 
AACATAACAG AGGAAGGTAC CTCACAAGTT AATGGGGGTG AATATAATCC TCAGATACTA 
ATGAATTGTC TTCTAGAAGT ACTTAATGAT AAAAGATGTC TGATTGTGTT GGATGGCCTA 
TGGAGCATAG ATGAAGGGCA ATTACTTCAT CTCAAGAGAA TGCTACAGAG CACACAGAAT 
ACCAAGATTA TAGTGACTAC TTGTAGTGAA AATGTTGCAA ATCTGATGCA TACTGTTCGT 
CCCTACAAAT TGGATCCTTT ATCTGAAGAA AATTGTTGGA CAATATTTTC TCAAAGGGTA 
TTTGGTGGTG GAGATAACTC AGACCTCACA CGAATTGGGA AGCAGATCGT GAACAAGTGT 
GAAGGTATAC CGGGAGTTAT TCATTCTCTT GGTGCATTCA TGCACGATAA CGGAACGAGC 
ACTTGGAATT GGAATGACAA AAATGAAGAG CTGTGGAAAT TGGAGAAACA GTTTCCATTG 
CAGATCAACT TGTTCTCATC AGTCAAACAG ATATATTACA AGATGCCATT GGCATTGAAA 
TCGTGCCTCA GCTATTTATC GATGTTTCCT AAAGGATCCA ATATTCGTAT GGAAAATGTT 
ATTACACAGT GGGCTGCACT TGGCATCCTT GGATCAACAC ATGGATCATT GCCTGTTTAT 
GCCCAGGGGA GGAAGTATAT CCAGGAACTG CTTTCTGTGT TCTTCCTTGA AGCACATGAT 
AAATCCACAG ATTTTGGAAT TAATCATGCT AGTGCCTCAA AAGTCTTTAA AATGCACAGT 
TTAGTTCATG AGTTTGCCAG ATATGTTACC AGTCATGATT TATTTATTCT GGATGGTGAA 
AGGATGCGTA ACGATAACCC AGGGAATCGT ACTTCTGGAT ATGCACTACT GACTAGGTGC 
AGTGATAAAT CCAAAGTTAG CAGAGGTTTC CTGAAAAGTG TAAGGGCAAT ATGTCTGAAG 
GACTGCAGGG GAACAAAGCT TATTGAGAAA ATATTCTCTG CTTTAAAGCA TCTACGTGTG 
TTGGACCTTA GTAGATGCTC ATTTCTGGAG TTACCTAGTT CTATTTGCCA GTTAACCCAT 
CTAAGGTACA TTGATATTTC TTGTTCGGCT ATTCAATCAC TACCTGATCA AATGAGTTCT 
GTACAACACC TTGAAGCACT GGATCTCTCG GGGACATGTA TTCAAGTCCT ACCTGATTTT 
GTTAGAACAT TTAAGAAGCT AACATATCTT AACCTACAAG AATGCTGGGA ACTCCGTCAC 
TTGCCCTCAA AGCTTGATGA TATTAAGAGT TTGCAGCATC TTAACCTGTC ATGCTGTCCT 
GCAGCTCACC AGCTAGTTGA ATCAATCAGT GGATTTCAAG AACTTCGGTT TTTGGATATA 
TCAAGTTGCA CAGAACTTCA AACATTGCCA GAATCCTTCG TCAGACTAAC AAATTTAGAG 
GACCTAATCC TGTCCAAATG CACCAGGCTC AAGAAGCTAC CAGAATCATT TGGTGATAAA 
CTCTGTTTTC TTCGGTTCTT GAACATATCA TATTGCTGTG AGCTAGAAGA AGTGCCTGCA 
TCTCTCGGAA GGCTTGCTAG TTTGGAGGTT CTTATACTTT CAGGGTGCAA CAGAATTCAG 
AATCTCCCTC AATCATTCAG TGACATTGCA TTCTTACGGA TGCTAGACCT TTCAGGGTGT 
GCAGATCTTC ACATGGACCT TGGAATGCTA CCAAATAATA ATTTGGAGAA TCTTAATGTG 
GATGGATGTT GTAAGGTCTA TGCTATGCCT GGGTGGACTG TGAACTTCCC TAAACTACTT 
CCTGAATGTC TACAAACTTG TGACCAACAT GTTCGACGCT TAATTGAACA CAAGCAAGCA 
TGTCCCAATC ATAGTGAATT AGAGATCAGC AATGAACTAA ATGTGCTTGA CGAAACTGGG 
GAAGCAATGG CAAGTCAATC ATTTCAACAT GCTGGTGACT CTGAATGTAC CACCAAGAAA 
GTCATTGAGG ACTCATCTGC GCATACAAGT CCTAAAATTC CTCTGCATGG CACTACCCCT 
ACTGGAGTCC AGCTGAAATC AGCTGGTTCG TTTTTTGGGG CTTCTACAAT TCGATTTTCG 
AAAATGTCTC GTTGTTTTAT GTGTACAACA GACTTGAAAA TGAATAGTCA AGGTGAAGGA 
GAACACATGG ACATGGTAAG AGTTTTTTCT TACAATGAGC TGCAAACAGC CACACATAAG 
TTTTCTGAGG CAAATAAGAT TGGTGAAGGT TCTATTGGTT CTGTTTTCAG GGGAAGGCTT 
AAAGATGGAA TGGCTGTCGC AGTAAAGTTG CTCCATGATT CAAAGCAGGG CAGGCGAGAG 
TTTTTAGCTG AACTCACAGC AATTTCTGGC ATTGTGCATG AAAACCTAAT CACCTTAGTT 
GGATGCTGTT GTGAAAGGTC CCATAGAATC CTTGTATACA ATTATCTTGA GAACAAAAGC 
CTTGCAGACA AACTGTTAGG ATCAAGCAGT AGCACCATCC AACTCAGCTG GAGAGCTCGT 
GTAAAAATTG CTATAGGTGT TGCTCGTGGG CTTGCTTTTC TTCACGAAGA AATCCGCCCC 
TCCATAATTC ACCGGGACAT AAAAGCGAGC CATATTCTTC TTGACAAGGA TTTCACCCCA 
AAAGTTTCTG GTTTTGGGTT AACAAGGCTC CTCCCACCAA TTACAACTTC TATGGCCACT 
CGAGTTGCAG GAACAATAGG ATACATGGCT CCTGAATATG CAATCAGGGG ACAAGTGACA 
AAGAAGGCGG ATATCTTTAG TTTTGGTATT GTGCTACTGG AGATTGTTAG TGGCAAATGT 
AATCACGATA GTAGATTACC CCAGGAAGAC CAATTTCTGC TTAAAAGGAC GTGGAGATCC 
TCTGAGGAAG GAAATCTGGT GGAAATCATA GATAAAGATT TAGGGGACGA CCTCGATGTT 
GAGGAGGCAT GCCGATTCTT GAATGTTGGT CTACTATGTA CTCAAAACGA GATGGCGAGG 
CGTCCCAACA TGAGGAACAT CGTAAAGATG CTGACTGGGG AGAAAGGAGT CTCCGTGGTC 
AAGGTGACAA GACCAATAAT GCTGTGGTCT GACTTGCTGG AATTAAAAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8067274 ( XM_021452918.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_021308593.1 Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSc112597
Clone ID Related Accession (Same CDS sequence)	XM_021452918.1
Accession Version	XM_021452918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	putative disease resistance protein RGA3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012871.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG TTAGCGCTGA TATAGATCGA CTCCAAAGGA GGCTTGCCTC CGACGGCCAA 
CGAATCAAGC TTCCCTCCAG CATACGGACG GATCTATCGC GCATGACAAG AGCTCTCTAC 
AGGTTGCAAG GCATCCTGAC TAATGTGGAG AAGCAACCAT TTGACGGGAG CAGAGAACCC 
TGCTTGAGCA AGATCAAACA GAATATTTAC GATGTGGAAG ATATACTAGA CGAGCTTGAA 
AATGGAAGCT TCAGGGCCCA ACGGAGCAGC AGGGGTTGGA AGCTAGCCGA GGCTTCAGAT 
CTCTGGTCAC AGGTAGCATT TTCCTTCTCA TCTAACCACT CAGTTCTGCA TTGTCGTATG 
ATGAATAAAA TGAAGAAGAT CAGAAAAAGT TTGGATCATG CATCAGAAGA CTCCTTCATA 
TTCACCTTAC TGCACCACAG AGCAGATGCA GATCAATCAG ATCACAATGT GTTTGATGAA 
AATGCAATCA TTGGAAGAAA TAAGGATAAG GAAAATGTAA GAGTCTTGCT CTTGAGTAAC 
AGTGAACAAA AAATTTCTAT TATTCCTATA GTTGGTCTTG GGGGATTGGG GAAAACAGCT 
CTTGCACAAC TCATTTTCAG TGACCAAGAT GAACGATATA ATTTTGACCT TCGTGTATGG 
ATCAATCTGA ACATGAGTTA TGATTTTAAC AGTATTGCTA GTGCTATAAT CTCAGAAGTT 
AACATAACAG AGGAAGGTAC CTCACAAGTT AATGGGGGTG AATATAATCC TCAGATACTA 
ATGAATTGTC TTCTAGAAGT ACTTAATGAT AAAAGATGTC TGATTGTGTT GGATGGCCTA 
TGGAGCATAG ATGAAGGGCA ATTACTTCAT CTCAAGAGAA TGCTACAGAG CACACAGAAT 
ACCAAGATTA TAGTGACTAC TTGTAGTGAA AATGTTGCAA ATCTGATGCA TACTGTTCGT 
CCCTACAAAT TGGATCCTTT ATCTGAAGAA AATTGTTGGA CAATATTTTC TCAAAGGGTA 
TTTGGTGGTG GAGATAACTC AGACCTCACA CGAATTGGGA AGCAGATCGT GAACAAGTGT 
GAAGGTATAC CGGGAGTTAT TCATTCTCTT GGTGCATTCA TGCACGATAA CGGAACGAGC 
ACTTGGAATT GGAATGACAA AAATGAAGAG CTGTGGAAAT TGGAGAAACA GTTTCCATTG 
CAGATCAACT TGTTCTCATC AGTCAAACAG ATATATTACA AGATGCCATT GGCATTGAAA 
TCGTGCCTCA GCTATTTATC GATGTTTCCT AAAGGATCCA ATATTCGTAT GGAAAATGTT 
ATTACACAGT GGGCTGCACT TGGCATCCTT GGATCAACAC ATGGATCATT GCCTGTTTAT 
GCCCAGGGGA GGAAGTATAT CCAGGAACTG CTTTCTGTGT TCTTCCTTGA AGCACATGAT 
AAATCCACAG ATTTTGGAAT TAATCATGCT AGTGCCTCAA AAGTCTTTAA AATGCACAGT 
TTAGTTCATG AGTTTGCCAG ATATGTTACC AGTCATGATT TATTTATTCT GGATGGTGAA 
AGGATGCGTA ACGATAACCC AGGGAATCGT ACTTCTGGAT ATGCACTACT GACTAGGTGC 
AGTGATAAAT CCAAAGTTAG CAGAGGTTTC CTGAAAAGTG TAAGGGCAAT ATGTCTGAAG 
GACTGCAGGG GAACAAAGCT TATTGAGAAA ATATTCTCTG CTTTAAAGCA TCTACGTGTG 
TTGGACCTTA GTAGATGCTC ATTTCTGGAG TTACCTAGTT CTATTTGCCA GTTAACCCAT 
CTAAGGTACA TTGATATTTC TTGTTCGGCT ATTCAATCAC TACCTGATCA AATGAGTTCT 
GTACAACACC TTGAAGCACT GGATCTCTCG GGGACATGTA TTCAAGTCCT ACCTGATTTT 
GTTAGAACAT TTAAGAAGCT AACATATCTT AACCTACAAG AATGCTGGGA ACTCCGTCAC 
TTGCCCTCAA AGCTTGATGA TATTAAGAGT TTGCAGCATC TTAACCTGTC ATGCTGTCCT 
GCAGCTCACC AGCTAGTTGA ATCAATCAGT GGATTTCAAG AACTTCGGTT TTTGGATATA 
TCAAGTTGCA CAGAACTTCA AACATTGCCA GAATCCTTCG TCAGACTAAC AAATTTAGAG 
GACCTAATCC TGTCCAAATG CACCAGGCTC AAGAAGCTAC CAGAATCATT TGGTGATAAA 
CTCTGTTTTC TTCGGTTCTT GAACATATCA TATTGCTGTG AGCTAGAAGA AGTGCCTGCA 
TCTCTCGGAA GGCTTGCTAG TTTGGAGGTT CTTATACTTT CAGGGTGCAA CAGAATTCAG 
AATCTCCCTC AATCATTCAG TGACATTGCA TTCTTACGGA TGCTAGACCT TTCAGGGTGT 
GCAGATCTTC ACATGGACCT TGGAATGCTA CCAAATAATA ATTTGGAGAA TCTTAATGTG 
GATGGATGTT GTAAGGTCTA TGCTATGCCT GGGTGGACTG TGAACTTCCC TAAACTACTT 
CCTGAATGTC TACAAACTTG TGACCAACAT GTTCGACGCT TAATTGAACA CAAGCAAGCA 
TGTCCCAATC ATAGTGAATT AGAGATCAGC AATGAACTAA ATGTGCTTGA CGAAACTGGG 
GAAGCAATGG CAAGTCAATC ATTTCAACAT GCTGGTGACT CTGAATGTAC CACCAAGAAA 
GTCATTGAGG ACTCATCTGC GCATACAAGT CCTAAAATTC CTCTGCATGG CACTACCCCT 
ACTGGAGTCC AGCTGAAATC AGCTGGTTCG TTTTTTGGGG CTTCTACAAT TCGATTTTCG 
AAAATGTCTC GTTGTTTTAT GTGTACAACA GACTTGAAAA TGAATAGTCA AGGAGAACAC 
ATGGACATGG TAAGAGTTTT TTCTTACAAT GAGCTGCAAA CAGCCACACA TAAGTTTTCT 
GAGGCAAATA AGATTGGTGA AGGTTCTATT GGTTCTGTTT TCAGGGGAAG GCTTAAAGAT 
GGAATGGCTG TCGCAGTAAA GTTGCTCCAT GATTCAAAGC AGGGCAGGCG AGAGTTTTTA 
GCTGAACTCA CAGCAATTTC TGGCATTGTG CATGAAAACC TAATCACCTT AGTTGGATGC 
TGTTGTGAAA GGTCCCATAG AATCCTTGTA TACAATTATC TTGAGAACAA AAGCCTTGCA 
GACAAACTGT TAGGATCAAG CAGTAGCACC ATCCAACTCA GCTGGAGAGC TCGTGTAAAA 
ATTGCTATAG GTGTTGCTCG TGGGCTTGCT TTTCTTCACG AAGAAATCCG CCCCTCCATA 
ATTCACCGGG ACATAAAAGC GAGCCATATT CTTCTTGACA AGGATTTCAC CCCAAAAGTT 
TCTGGTTTTG GGTTAACAAG GCTCCTCCCA CCAATTACAA CTTCTATGGC CACTCGAGTT 
GCAGGAACAA TAGGATACAT GGCTCCTGAA TATGCAATCA GGGGACAAGT GACAAAGAAG 
GCGGATATCT TTAGTTTTGG TATTGTGCTA CTGGAGATTG TTAGTGGCAA ATGTAATCAC 
GATAGTAGAT TACCCCAGGA AGACCAATTT CTGCTTAAAA GGACGTGGAG ATCCTCTGAG 
GAAGGAAATC TGGTGGAAAT CATAGATAAA GATTTAGGGG ACGACCTCGA TGTTGAGGAG 
GCATGCCGAT TCTTGAATGT TGGTCTACTA TGTACTCAAA ACGAGATGGC GAGGCGTCCC 
AACATGAGGA ACATCGTAAA GATGCTGACT GGGGAGAAAG GAGTCTCCGT GGTCAAGGTG 
ACAAGACCAA TAATGCTGTG GTCTGACTTG CTGGAATTAA AAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021308593.1
CDS	193..4020
Translation	MATVSADIDRLQRRLASDGQRIKLPSSIRTDLSRMTRALYRLQGILTNVEKQPFDGSREPCLSKIKQNIYDVEDILDELENGSFRAQRSSRGWKLAEASDLWSQVAFSFSSNHSVLHCRMMNKMKKIRKSLDHASEDSFIFTLLHHRADADQSDHNVFDENAIIGRNKDKENVRVLLLSNSEQKISIIPIVGLGGLGKTALAQLIFSDQDERYNFDLRVWINLNMSYDFNSIASAIISEVNITEEGTSQVNGGEYNPQILMNCLLEVLNDKRCLIVLDGLWSIDEGQLLHLKRMLQSTQNTKIIVTTCSENVANLMHTVRPYKLDPLSEENCWTIFSQRVFGGGDNSDLTRIGKQIVNKCEGIPGVIHSLGAFMHDNGTSTWNWNDKNEELWKLEKQFPLQINLFSSVKQIYYKMPLALKSCLSYLSMFPKGSNIRMENVITQWAALGILGSTHGSLPVYAQGRKYIQELLSVFFLEAHDKSTDFGINHASASKVFKMHSLVHEFARYVTSHDLFILDGERMRNDNPGNRTSGYALLTRCSDKSKVSRGFLKSVRAICLKDCRGTKLIEKIFSALKHLRVLDLSRCSFLELPSSICQLTHLRYIDISCSAIQSLPDQMSSVQHLEALDLSGTCIQVLPDFVRTFKKLTYLNLQECWELRHLPSKLDDIKSLQHLNLSCCPAAHQLVESISGFQELRFLDISSCTELQTLPESFVRLTNLEDLILSKCTRLKKLPESFGDKLCFLRFLNISYCCELEEVPASLGRLASLEVLILSGCNRIQNLPQSFSDIAFLRMLDLSGCADLHMDLGMLPNNNLENLNVDGCCKVYAMPGWTVNFPKLLPECLQTCDQHVRRLIEHKQACPNHSELEISNELNVLDETGEAMASQSFQHAGDSECTTKKVIEDSSAHTSPKIPLHGTTPTGVQLKSAGSFFGASTIRFSKMSRCFMCTTDLKMNSQGEHMDMVRVFSYNELQTATHKFSEANKIGEGSIGSVFRGRLKDGMAVAVKLLHDSKQGRREFLAELTAISGIVHENLITLVGCCCERSHRILVYNYLENKSLADKLLGSSSSTIQLSWRARVKIAIGVARGLAFLHEEIRPSIIHRDIKASHILLDKDFTPKVSGFGLTRLLPPITTSMATRVAGTIGYMAPEYAIRGQVTKKADIFSFGIVLLEIVSGKCNHDSRLPQEDQFLLKRTWRSSEEGNLVEIIDKDLGDDLDVEEACRFLNVGLLCTQNEMARRPNMRNIVKMLTGEKGVSVVKVTRPIMLWSDLLELKT

Target ORF information:

RefSeq Version	XM_021452918.1
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021452918.1

ORF Insert Sequence:

ATGGCCACTG TTAGCGCTGA TATAGATCGA CTCCAAAGGA GGCTTGCCTC CGACGGCCAA 
CGAATCAAGC TTCCCTCCAG CATACGGACG GATCTATCGC GCATGACAAG AGCTCTCTAC 
AGGTTGCAAG GCATCCTGAC TAATGTGGAG AAGCAACCAT TTGACGGGAG CAGAGAACCC 
TGCTTGAGCA AGATCAAACA GAATATTTAC GATGTGGAAG ATATACTAGA CGAGCTTGAA 
AATGGAAGCT TCAGGGCCCA ACGGAGCAGC AGGGGTTGGA AGCTAGCCGA GGCTTCAGAT 
CTCTGGTCAC AGGTAGCATT TTCCTTCTCA TCTAACCACT CAGTTCTGCA TTGTCGTATG 
ATGAATAAAA TGAAGAAGAT CAGAAAAAGT TTGGATCATG CATCAGAAGA CTCCTTCATA 
TTCACCTTAC TGCACCACAG AGCAGATGCA GATCAATCAG ATCACAATGT GTTTGATGAA 
AATGCAATCA TTGGAAGAAA TAAGGATAAG GAAAATGTAA GAGTCTTGCT CTTGAGTAAC 
AGTGAACAAA AAATTTCTAT TATTCCTATA GTTGGTCTTG GGGGATTGGG GAAAACAGCT 
CTTGCACAAC TCATTTTCAG TGACCAAGAT GAACGATATA ATTTTGACCT TCGTGTATGG 
ATCAATCTGA ACATGAGTTA TGATTTTAAC AGTATTGCTA GTGCTATAAT CTCAGAAGTT 
AACATAACAG AGGAAGGTAC CTCACAAGTT AATGGGGGTG AATATAATCC TCAGATACTA 
ATGAATTGTC TTCTAGAAGT ACTTAATGAT AAAAGATGTC TGATTGTGTT GGATGGCCTA 
TGGAGCATAG ATGAAGGGCA ATTACTTCAT CTCAAGAGAA TGCTACAGAG CACACAGAAT 
ACCAAGATTA TAGTGACTAC TTGTAGTGAA AATGTTGCAA ATCTGATGCA TACTGTTCGT 
CCCTACAAAT TGGATCCTTT ATCTGAAGAA AATTGTTGGA CAATATTTTC TCAAAGGGTA 
TTTGGTGGTG GAGATAACTC AGACCTCACA CGAATTGGGA AGCAGATCGT GAACAAGTGT 
GAAGGTATAC CGGGAGTTAT TCATTCTCTT GGTGCATTCA TGCACGATAA CGGAACGAGC 
ACTTGGAATT GGAATGACAA AAATGAAGAG CTGTGGAAAT TGGAGAAACA GTTTCCATTG 
CAGATCAACT TGTTCTCATC AGTCAAACAG ATATATTACA AGATGCCATT GGCATTGAAA 
TCGTGCCTCA GCTATTTATC GATGTTTCCT AAAGGATCCA ATATTCGTAT GGAAAATGTT 
ATTACACAGT GGGCTGCACT TGGCATCCTT GGATCAACAC ATGGATCATT GCCTGTTTAT 
GCCCAGGGGA GGAAGTATAT CCAGGAACTG CTTTCTGTGT TCTTCCTTGA AGCACATGAT 
AAATCCACAG ATTTTGGAAT TAATCATGCT AGTGCCTCAA AAGTCTTTAA AATGCACAGT 
TTAGTTCATG AGTTTGCCAG ATATGTTACC AGTCATGATT TATTTATTCT GGATGGTGAA 
AGGATGCGTA ACGATAACCC AGGGAATCGT ACTTCTGGAT ATGCACTACT GACTAGGTGC 
AGTGATAAAT CCAAAGTTAG CAGAGGTTTC CTGAAAAGTG TAAGGGCAAT ATGTCTGAAG 
GACTGCAGGG GAACAAAGCT TATTGAGAAA ATATTCTCTG CTTTAAAGCA TCTACGTGTG 
TTGGACCTTA GTAGATGCTC ATTTCTGGAG TTACCTAGTT CTATTTGCCA GTTAACCCAT 
CTAAGGTACA TTGATATTTC TTGTTCGGCT ATTCAATCAC TACCTGATCA AATGAGTTCT 
GTACAACACC TTGAAGCACT GGATCTCTCG GGGACATGTA TTCAAGTCCT ACCTGATTTT 
GTTAGAACAT TTAAGAAGCT AACATATCTT AACCTACAAG AATGCTGGGA ACTCCGTCAC 
TTGCCCTCAA AGCTTGATGA TATTAAGAGT TTGCAGCATC TTAACCTGTC ATGCTGTCCT 
GCAGCTCACC AGCTAGTTGA ATCAATCAGT GGATTTCAAG AACTTCGGTT TTTGGATATA 
TCAAGTTGCA CAGAACTTCA AACATTGCCA GAATCCTTCG TCAGACTAAC AAATTTAGAG 
GACCTAATCC TGTCCAAATG CACCAGGCTC AAGAAGCTAC CAGAATCATT TGGTGATAAA 
CTCTGTTTTC TTCGGTTCTT GAACATATCA TATTGCTGTG AGCTAGAAGA AGTGCCTGCA 
TCTCTCGGAA GGCTTGCTAG TTTGGAGGTT CTTATACTTT CAGGGTGCAA CAGAATTCAG 
AATCTCCCTC AATCATTCAG TGACATTGCA TTCTTACGGA TGCTAGACCT TTCAGGGTGT 
GCAGATCTTC ACATGGACCT TGGAATGCTA CCAAATAATA ATTTGGAGAA TCTTAATGTG 
GATGGATGTT GTAAGGTCTA TGCTATGCCT GGGTGGACTG TGAACTTCCC TAAACTACTT 
CCTGAATGTC TACAAACTTG TGACCAACAT GTTCGACGCT TAATTGAACA CAAGCAAGCA 
TGTCCCAATC ATAGTGAATT AGAGATCAGC AATGAACTAA ATGTGCTTGA CGAAACTGGG 
GAAGCAATGG CAAGTCAATC ATTTCAACAT GCTGGTGACT CTGAATGTAC CACCAAGAAA 
GTCATTGAGG ACTCATCTGC GCATACAAGT CCTAAAATTC CTCTGCATGG CACTACCCCT 
ACTGGAGTCC AGCTGAAATC AGCTGGTTCG TTTTTTGGGG CTTCTACAAT TCGATTTTCG 
AAAATGTCTC GTTGTTTTAT GTGTACAACA GACTTGAAAA TGAATAGTCA AGGAGAACAC 
ATGGACATGG TAAGAGTTTT TTCTTACAAT GAGCTGCAAA CAGCCACACA TAAGTTTTCT 
GAGGCAAATA AGATTGGTGA AGGTTCTATT GGTTCTGTTT TCAGGGGAAG GCTTAAAGAT 
GGAATGGCTG TCGCAGTAAA GTTGCTCCAT GATTCAAAGC AGGGCAGGCG AGAGTTTTTA 
GCTGAACTCA CAGCAATTTC TGGCATTGTG CATGAAAACC TAATCACCTT AGTTGGATGC 
TGTTGTGAAA GGTCCCATAG AATCCTTGTA TACAATTATC TTGAGAACAA AAGCCTTGCA 
GACAAACTGT TAGGATCAAG CAGTAGCACC ATCCAACTCA GCTGGAGAGC TCGTGTAAAA 
ATTGCTATAG GTGTTGCTCG TGGGCTTGCT TTTCTTCACG AAGAAATCCG CCCCTCCATA 
ATTCACCGGG ACATAAAAGC GAGCCATATT CTTCTTGACA AGGATTTCAC CCCAAAAGTT 
TCTGGTTTTG GGTTAACAAG GCTCCTCCCA CCAATTACAA CTTCTATGGC CACTCGAGTT 
GCAGGAACAA TAGGATACAT GGCTCCTGAA TATGCAATCA GGGGACAAGT GACAAAGAAG 
GCGGATATCT TTAGTTTTGG TATTGTGCTA CTGGAGATTG TTAGTGGCAA ATGTAATCAC 
GATAGTAGAT TACCCCAGGA AGACCAATTT CTGCTTAAAA GGACGTGGAG ATCCTCTGAG 
GAAGGAAATC TGGTGGAAAT CATAGATAAA GATTTAGGGG ACGACCTCGA TGTTGAGGAG 
GCATGCCGAT TCTTGAATGT TGGTCTACTA TGTACTCAAA ACGAGATGGC GAGGCGTCCC 
AACATGAGGA ACATCGTAAA GATGCTGACT GGGGAGAAAG GAGTCTCCGT GGTCAAGGTG 
ACAAGACCAA TAATGCTGTG GTCTGACTTG CTGGAATTAA AAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8067274 ( XM_021452919.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_021308594.1 Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X4, mRNA.

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CloneID	OSc112598
Clone ID Related Accession (Same CDS sequence)	XM_021452919.1
Accession Version	XM_021452919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	putative disease resistance protein RGA3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012871.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG TTAGCGCTGA TATAGATCGA CTCCAAAGGA GGCTTGCCTC CGACGGCCAA 
CGAATCAAGC TTCCCTCCAG CATACGGACG GATCTATCGC GCATGACAAG AGCTCTCTAC 
AGGTTGCAAG GCATCCTGAC TAATGTGGAG AAGCAACCAT TTGACGGGAG CAGAGAACCC 
TGCTTGAGCA AGATCAAACA GAATATTTAC GATGTGGAAG ATATACTAGA CGAGCTTGAA 
AATGGAAGCT TCAGGGCCCA ACGGAGCAGC AGGGGTTGGA AGCTAGCCGA GGCTTCAGAT 
CTCTGGTCAC AGGTAGCATT TTCCTTCTCA TCTAACCACT CAGTTCTGCA TTGTCGTATG 
ATGAATAAAA TGAAGAAGAT CAGAAAAAGT TTGGATCATG CATCAGAAGA CTCCTTCATA 
TTCACCTTAC TGCACCACAG AGCAGATGCA GATCAATCAG ATCACAATGT GTTTGATGAA 
AATGCAATCA TTGGAAGAAA TAAGGATAAG GAAAATGTAA GAGTCTTGCT CTTGAGTAAC 
AGTGAACAAA AAATTTCTAT TATTCCTATA GTTGGTCTTG GGGGATTGGG GAAAACAGCT 
CTTGCACAAC TCATTTTCAG TGACCAAGAT GAACGATATA ATTTTGACCT TCGTGTATGG 
ATCAATCTGA ACATGAGTTA TGATTTTAAC AGTATTGCTA GTGCTATAAT CTCAGAAGTT 
AACATAACAG AGGAAGGTAC CTCACAAGTT AATGGGGGTG AATATAATCC TCAGATACTA 
ATGAATTGTC TTCTAGAAGT ACTTAATGAT AAAAGATGTC TGATTGTGTT GGATGGCCTA 
TGGAGCATAG ATGAAGGGCA ATTACTTCAT CTCAAGAGAA TGCTACAGAG CACACAGAAT 
ACCAAGATTA TAGTGACTAC TTGTAGTGAA AATGTTGCAA ATCTGATGCA TACTGTTCGT 
CCCTACAAAT TGGATCCTTT ATCTGAAGAA AATTGTTGGA CAATATTTTC TCAAAGGGTA 
TTTGGTGGTG GAGATAACTC AGACCTCACA CGAATTGGGA AGCAGATCGT GAACAAGTGT 
GAAGGTATAC CGGGAGTTAT TCATTCTCTT GGTGCATTCA TGCACGATAA CGGAACGAGC 
ACTTGGAATT GGAATGACAA AAATGAAGAG CTGTGGAAAT TGGAGAAACA GTTTCCATTG 
CAGATCAACT TGTTCTCATC AGTCAAACAG ATATATTACA AGATGCCATT GGCATTGAAA 
TCGTGCCTCA GCTATTTATC GATGTTTCCT AAAGGATCCA ATATTCGTAT GGAAAATGTT 
ATTACACAGT GGGCTGCACT TGGCATCCTT GGATCAACAC ATGGATCATT GCCTGTTTAT 
GCCCAGGGGA GGAAGTATAT CCAGGAACTG CTTTCTGTGT TCTTCCTTGA AGCACATGAT 
AAATCCACAG ATTTTGGAAT TAATCATGCT AGTGCCTCAA AAGTCTTTAA AATGCACAGT 
TTAGTTCATG AGTTTGCCAG ATATGTTACC AGTCATGATT TATTTATTCT GGATGGTGAA 
AGGATGCGTA ACGATAACCC AGGGAATCGT ACTTCTGGAT ATGCACTACT GACTAGGTGC 
AGTGATAAAT CCAAAGTTAG CAGAGGTTTC CTGAAAAGTG TAAGGGCAAT ATGTCTGAAG 
GACTGCAGGG GAACAAAGCT TATTGAGAAA ATATTCTCTG CTTTAAAGCA TCTACGTGTG 
TTGGACCTTA GTAGATGCTC ATTTCTGGAG TTACCTAGTT CTATTTGCCA GTTAACCCAT 
CTAAGGTACA TTGATATTTC TTGTTCGGCT ATTCAATCAC TACCTGATCA AATGAGTTCT 
GTACAACACC TTGAAGCACT GGATCTCTCG GGGACATGTA TTCAAGTCCT ACCTGATTTT 
GTTAGAACAT TTAAGAAGCT AACATATCTT AACCTACAAG AATGCTGGGA ACTCCGTCAC 
TTGCCCTCAA AGCTTGATGA TATTAAGAGT TTGCAGCATC TTAACCTGTC ATGCTGTCCT 
GCAGCTCACC AGCTAGTTGA ATCAATCAGT GGATTTCAAG AACTTCGGTT TTTGGATATA 
TCAAGTTGCA CAGAACTTCA AACATTGCCA GAATCCTTCG TCAGACTAAC AAATTTAGAG 
GACCTAATCC TGTCCAAATG CACCAGGCTC AAGAAGCTAC CAGAATCATT TGGTGATAAA 
CTCTGTTTTC TTCGGTTCTT GAACATATCA TATTGCTGTG AGCTAGAAGA AGTGCCTGCA 
TCTCTCGGAA GGCTTGCTAG TTTGGAGGTT CTTATACTTT CAGGGTGCAA CAGAATTCAG 
AATCTCCCTC AATCATTCAG TGACATTGCA TTCTTACGGA TGCTAGACCT TTCAGGGTGT 
GCAGATCTTC ACATGGACCT TGGAATGCTA CCAAATAATA ATTTGGAGAA TCTTAATGTG 
GATGGATGTT GTAAGGTCTA TGCTATGCCT GGGTGGACTG TGAACTTCCC TAAACTACTT 
CCTGAATGTC TACAAACTTG TGACCAACAT GTTCGACGCT TAATTGAACA CAAGCAAGCA 
TGTCCCAATC ATAGTGAATT AGAGATCAGC AATGAACTAA ATGTGCTTGA CGAAACTGGG 
GAAGCAATGG CAAGTCAATC ATTTCAACAT GCTGGTGACT CTGAATGTAC CACCAAGAAA 
GTCATTGAGG ACTCATCTGC GCATACAAGT CCTAAAATTC CTCTGCATGG CACTACCCCT 
ACTGGAGTCC AGCTGAAATC AGCTGGTTCG TTTTTTGGGG CTTCTACAAT TCGATTTTCG 
AAAATGTCTC GTTGTTTTAT GTGTACAACA GACTTGAAAA TGAATAGTCA AGGTGAAGGT 
GAAGGAGAAC ACATGGACAT GGGAAGGCTT AAAGATGGAA TGGCTGTCGC AGTAAAGTTG 
CTCCATGATT CAAAGCAGGG CAGGCGAGAG TTTTTAGCTG AACTCACAGC AATTTCTGGC 
ATTGTGCATG AAAACCTAAT CACCTTAGTT GGATGCTGTT GTGAAAGGTC CCATAGAATC 
CTTGTATACA ATTATCTTGA GAACAAAAGC CTTGCAGACA AACTGTTAGG ATCAAGCAGT 
AGCACCATCC AACTCAGCTG GAGAGCTCGT GTAAAAATTG CTATAGGTGT TGCTCGTGGG 
CTTGCTTTTC TTCACGAAGA AATCCGCCCC TCCATAATTC ACCGGGACAT AAAAGCGAGC 
CATATTCTTC TTGACAAGGA TTTCACCCCA AAAGTTTCTG GTTTTGGGTT AACAAGGCTC 
CTCCCACCAA TTACAACTTC TATGGCCACT CGAGTTGCAG GAACAATAGG ATACATGGCT 
CCTGAATATG CAATCAGGGG ACAAGTGACA AAGAAGGCGG ATATCTTTAG TTTTGGTATT 
GTGCTACTGG AGATTGTTAG TGGCAAATGT AATCACGATA GTAGATTACC CCAGGAAGAC 
CAATTTCTGC TTAAAAGGAC GTGGAGATCC TCTGAGGAAG GAAATCTGGT GGAAATCATA 
GATAAAGATT TAGGGGACGA CCTCGATGTT GAGGAGGCAT GCCGATTCTT GAATGTTGGT 
CTACTATGTA CTCAAAACGA GATGGCGAGG CGTCCCAACA TGAGGAACAT CGTAAAGATG 
CTGACTGGGG AGAAAGGAGT CTCCGTGGTC AAGGTGACAA GACCAATAAT GCTGTGGTCT 
GACTTGCTGG AATTAAAAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021308594.1
CDS	194..3937
Translation	MATVSADIDRLQRRLASDGQRIKLPSSIRTDLSRMTRALYRLQGILTNVEKQPFDGSREPCLSKIKQNIYDVEDILDELENGSFRAQRSSRGWKLAEASDLWSQVAFSFSSNHSVLHCRMMNKMKKIRKSLDHASEDSFIFTLLHHRADADQSDHNVFDENAIIGRNKDKENVRVLLLSNSEQKISIIPIVGLGGLGKTALAQLIFSDQDERYNFDLRVWINLNMSYDFNSIASAIISEVNITEEGTSQVNGGEYNPQILMNCLLEVLNDKRCLIVLDGLWSIDEGQLLHLKRMLQSTQNTKIIVTTCSENVANLMHTVRPYKLDPLSEENCWTIFSQRVFGGGDNSDLTRIGKQIVNKCEGIPGVIHSLGAFMHDNGTSTWNWNDKNEELWKLEKQFPLQINLFSSVKQIYYKMPLALKSCLSYLSMFPKGSNIRMENVITQWAALGILGSTHGSLPVYAQGRKYIQELLSVFFLEAHDKSTDFGINHASASKVFKMHSLVHEFARYVTSHDLFILDGERMRNDNPGNRTSGYALLTRCSDKSKVSRGFLKSVRAICLKDCRGTKLIEKIFSALKHLRVLDLSRCSFLELPSSICQLTHLRYIDISCSAIQSLPDQMSSVQHLEALDLSGTCIQVLPDFVRTFKKLTYLNLQECWELRHLPSKLDDIKSLQHLNLSCCPAAHQLVESISGFQELRFLDISSCTELQTLPESFVRLTNLEDLILSKCTRLKKLPESFGDKLCFLRFLNISYCCELEEVPASLGRLASLEVLILSGCNRIQNLPQSFSDIAFLRMLDLSGCADLHMDLGMLPNNNLENLNVDGCCKVYAMPGWTVNFPKLLPECLQTCDQHVRRLIEHKQACPNHSELEISNELNVLDETGEAMASQSFQHAGDSECTTKKVIEDSSAHTSPKIPLHGTTPTGVQLKSAGSFFGASTIRFSKMSRCFMCTTDLKMNSQGEGEGEHMDMGRLKDGMAVAVKLLHDSKQGRREFLAELTAISGIVHENLITLVGCCCERSHRILVYNYLENKSLADKLLGSSSSTIQLSWRARVKIAIGVARGLAFLHEEIRPSIIHRDIKASHILLDKDFTPKVSGFGLTRLLPPITTSMATRVAGTIGYMAPEYAIRGQVTKKADIFSFGIVLLEIVSGKCNHDSRLPQEDQFLLKRTWRSSEEGNLVEIIDKDLGDDLDVEEACRFLNVGLLCTQNEMARRPNMRNIVKMLTGEKGVSVVKVTRPIMLWSDLLELKT

Target ORF information:

RefSeq Version	XM_021452919.1
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021452919.1

ORF Insert Sequence:

ATGGCCACTG TTAGCGCTGA TATAGATCGA CTCCAAAGGA GGCTTGCCTC CGACGGCCAA 
CGAATCAAGC TTCCCTCCAG CATACGGACG GATCTATCGC GCATGACAAG AGCTCTCTAC 
AGGTTGCAAG GCATCCTGAC TAATGTGGAG AAGCAACCAT TTGACGGGAG CAGAGAACCC 
TGCTTGAGCA AGATCAAACA GAATATTTAC GATGTGGAAG ATATACTAGA CGAGCTTGAA 
AATGGAAGCT TCAGGGCCCA ACGGAGCAGC AGGGGTTGGA AGCTAGCCGA GGCTTCAGAT 
CTCTGGTCAC AGGTAGCATT TTCCTTCTCA TCTAACCACT CAGTTCTGCA TTGTCGTATG 
ATGAATAAAA TGAAGAAGAT CAGAAAAAGT TTGGATCATG CATCAGAAGA CTCCTTCATA 
TTCACCTTAC TGCACCACAG AGCAGATGCA GATCAATCAG ATCACAATGT GTTTGATGAA 
AATGCAATCA TTGGAAGAAA TAAGGATAAG GAAAATGTAA GAGTCTTGCT CTTGAGTAAC 
AGTGAACAAA AAATTTCTAT TATTCCTATA GTTGGTCTTG GGGGATTGGG GAAAACAGCT 
CTTGCACAAC TCATTTTCAG TGACCAAGAT GAACGATATA ATTTTGACCT TCGTGTATGG 
ATCAATCTGA ACATGAGTTA TGATTTTAAC AGTATTGCTA GTGCTATAAT CTCAGAAGTT 
AACATAACAG AGGAAGGTAC CTCACAAGTT AATGGGGGTG AATATAATCC TCAGATACTA 
ATGAATTGTC TTCTAGAAGT ACTTAATGAT AAAAGATGTC TGATTGTGTT GGATGGCCTA 
TGGAGCATAG ATGAAGGGCA ATTACTTCAT CTCAAGAGAA TGCTACAGAG CACACAGAAT 
ACCAAGATTA TAGTGACTAC TTGTAGTGAA AATGTTGCAA ATCTGATGCA TACTGTTCGT 
CCCTACAAAT TGGATCCTTT ATCTGAAGAA AATTGTTGGA CAATATTTTC TCAAAGGGTA 
TTTGGTGGTG GAGATAACTC AGACCTCACA CGAATTGGGA AGCAGATCGT GAACAAGTGT 
GAAGGTATAC CGGGAGTTAT TCATTCTCTT GGTGCATTCA TGCACGATAA CGGAACGAGC 
ACTTGGAATT GGAATGACAA AAATGAAGAG CTGTGGAAAT TGGAGAAACA GTTTCCATTG 
CAGATCAACT TGTTCTCATC AGTCAAACAG ATATATTACA AGATGCCATT GGCATTGAAA 
TCGTGCCTCA GCTATTTATC GATGTTTCCT AAAGGATCCA ATATTCGTAT GGAAAATGTT 
ATTACACAGT GGGCTGCACT TGGCATCCTT GGATCAACAC ATGGATCATT GCCTGTTTAT 
GCCCAGGGGA GGAAGTATAT CCAGGAACTG CTTTCTGTGT TCTTCCTTGA AGCACATGAT 
AAATCCACAG ATTTTGGAAT TAATCATGCT AGTGCCTCAA AAGTCTTTAA AATGCACAGT 
TTAGTTCATG AGTTTGCCAG ATATGTTACC AGTCATGATT TATTTATTCT GGATGGTGAA 
AGGATGCGTA ACGATAACCC AGGGAATCGT ACTTCTGGAT ATGCACTACT GACTAGGTGC 
AGTGATAAAT CCAAAGTTAG CAGAGGTTTC CTGAAAAGTG TAAGGGCAAT ATGTCTGAAG 
GACTGCAGGG GAACAAAGCT TATTGAGAAA ATATTCTCTG CTTTAAAGCA TCTACGTGTG 
TTGGACCTTA GTAGATGCTC ATTTCTGGAG TTACCTAGTT CTATTTGCCA GTTAACCCAT 
CTAAGGTACA TTGATATTTC TTGTTCGGCT ATTCAATCAC TACCTGATCA AATGAGTTCT 
GTACAACACC TTGAAGCACT GGATCTCTCG GGGACATGTA TTCAAGTCCT ACCTGATTTT 
GTTAGAACAT TTAAGAAGCT AACATATCTT AACCTACAAG AATGCTGGGA ACTCCGTCAC 
TTGCCCTCAA AGCTTGATGA TATTAAGAGT TTGCAGCATC TTAACCTGTC ATGCTGTCCT 
GCAGCTCACC AGCTAGTTGA ATCAATCAGT GGATTTCAAG AACTTCGGTT TTTGGATATA 
TCAAGTTGCA CAGAACTTCA AACATTGCCA GAATCCTTCG TCAGACTAAC AAATTTAGAG 
GACCTAATCC TGTCCAAATG CACCAGGCTC AAGAAGCTAC CAGAATCATT TGGTGATAAA 
CTCTGTTTTC TTCGGTTCTT GAACATATCA TATTGCTGTG AGCTAGAAGA AGTGCCTGCA 
TCTCTCGGAA GGCTTGCTAG TTTGGAGGTT CTTATACTTT CAGGGTGCAA CAGAATTCAG 
AATCTCCCTC AATCATTCAG TGACATTGCA TTCTTACGGA TGCTAGACCT TTCAGGGTGT 
GCAGATCTTC ACATGGACCT TGGAATGCTA CCAAATAATA ATTTGGAGAA TCTTAATGTG 
GATGGATGTT GTAAGGTCTA TGCTATGCCT GGGTGGACTG TGAACTTCCC TAAACTACTT 
CCTGAATGTC TACAAACTTG TGACCAACAT GTTCGACGCT TAATTGAACA CAAGCAAGCA 
TGTCCCAATC ATAGTGAATT AGAGATCAGC AATGAACTAA ATGTGCTTGA CGAAACTGGG 
GAAGCAATGG CAAGTCAATC ATTTCAACAT GCTGGTGACT CTGAATGTAC CACCAAGAAA 
GTCATTGAGG ACTCATCTGC GCATACAAGT CCTAAAATTC CTCTGCATGG CACTACCCCT 
ACTGGAGTCC AGCTGAAATC AGCTGGTTCG TTTTTTGGGG CTTCTACAAT TCGATTTTCG 
AAAATGTCTC GTTGTTTTAT GTGTACAACA GACTTGAAAA TGAATAGTCA AGGTGAAGGT 
GAAGGAGAAC ACATGGACAT GGGAAGGCTT AAAGATGGAA TGGCTGTCGC AGTAAAGTTG 
CTCCATGATT CAAAGCAGGG CAGGCGAGAG TTTTTAGCTG AACTCACAGC AATTTCTGGC 
ATTGTGCATG AAAACCTAAT CACCTTAGTT GGATGCTGTT GTGAAAGGTC CCATAGAATC 
CTTGTATACA ATTATCTTGA GAACAAAAGC CTTGCAGACA AACTGTTAGG ATCAAGCAGT 
AGCACCATCC AACTCAGCTG GAGAGCTCGT GTAAAAATTG CTATAGGTGT TGCTCGTGGG 
CTTGCTTTTC TTCACGAAGA AATCCGCCCC TCCATAATTC ACCGGGACAT AAAAGCGAGC 
CATATTCTTC TTGACAAGGA TTTCACCCCA AAAGTTTCTG GTTTTGGGTT AACAAGGCTC 
CTCCCACCAA TTACAACTTC TATGGCCACT CGAGTTGCAG GAACAATAGG ATACATGGCT 
CCTGAATATG CAATCAGGGG ACAAGTGACA AAGAAGGCGG ATATCTTTAG TTTTGGTATT 
GTGCTACTGG AGATTGTTAG TGGCAAATGT AATCACGATA GTAGATTACC CCAGGAAGAC 
CAATTTCTGC TTAAAAGGAC GTGGAGATCC TCTGAGGAAG GAAATCTGGT GGAAATCATA 
GATAAAGATT TAGGGGACGA CCTCGATGTT GAGGAGGCAT GCCGATTCTT GAATGTTGGT 
CTACTATGTA CTCAAAACGA GATGGCGAGG CGTCCCAACA TGAGGAACAT CGTAAAGATG 
CTGACTGGGG AGAAAGGAGT CTCCGTGGTC AAGGTGACAA GACCAATAAT GCTGTGGTCT 
GACTTGCTGG AATTAAAAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8067274 ( XM_021452920.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_021308595.1 Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X5, mRNA.

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CloneID	OSc112599
Clone ID Related Accession (Same CDS sequence)	XM_021452920.1
Accession Version	XM_021452920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	putative disease resistance protein RGA3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012871.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG TTAGCGCTGA TATAGATCGA CTCCAAAGGA GGCTTGCCTC CGACGGCCAA 
CGAATCAAGC TTCCCTCCAG CATACGGACG GATCTATCGC GCATGACAAG AGCTCTCTAC 
AGGTTGCAAG GCATCCTGAC TAATGTGGAG AAGCAACCAT TTGACGGGAG CAGAGAACCC 
TGCTTGAGCA AGATCAAACA GAATATTTAC GATGTGGAAG ATATACTAGA CGAGCTTGAA 
AATGGAAGCT TCAGGGCCCA ACGGAGCAGC AGGGGTTGGA AGCTAGCCGA GGCTTCAGAT 
CTCTGGTCAC AGGTAGCATT TTCCTTCTCA TCTAACCACT CAGTTCTGCA TTGTCGTATG 
ATGAATAAAA TGAAGAAGAT CAGAAAAAGT TTGGATCATG CATCAGAAGA CTCCTTCATA 
TTCACCTTAC TGCACCACAG AGCAGATGCA GATCAATCAG ATCACAATGT GTTTGATGAA 
AATGCAATCA TTGGAAGAAA TAAGGATAAG GAAAATGTAA GAGTCTTGCT CTTGAGTAAC 
AGTGAACAAA AAATTTCTAT TATTCCTATA GTTGGTCTTG GGGGATTGGG GAAAACAGCT 
CTTGCACAAC TCATTTTCAG TGACCAAGAT GAACGATATA ATTTTGACCT TCGTGTATGG 
ATCAATCTGA ACATGAGTTA TGATTTTAAC AGTATTGCTA GTGCTATAAT CTCAGAAGTT 
AACATAACAG AGGAAGGTAC CTCACAAGTT AATGGGGGTG AATATAATCC TCAGATACTA 
ATGAATTGTC TTCTAGAAGT ACTTAATGAT AAAAGATGTC TGATTGTGTT GGATGGCCTA 
TGGAGCATAG ATGAAGGGCA ATTACTTCAT CTCAAGAGAA TGCTACAGAG CACACAGAAT 
ACCAAGATTA TAGTGACTAC TTGTAGTGAA AATGTTGCAA ATCTGATGCA TACTGTTCGT 
CCCTACAAAT TGGATCCTTT ATCTGAAGAA AATTGTTGGA CAATATTTTC TCAAAGGGTA 
TTTGGTGGTG GAGATAACTC AGACCTCACA CGAATTGGGA AGCAGATCGT GAACAAGTGT 
GAAGGTATAC CGGGAGTTAT TCATTCTCTT GGTGCATTCA TGCACGATAA CGGAACGAGC 
ACTTGGAATT GGAATGACAA AAATGAAGAG CTGTGGAAAT TGGAGAAACA GTTTCCATTG 
CAGATCAACT TGTTCTCATC AGTCAAACAG ATATATTACA AGATGCCATT GGCATTGAAA 
TCGTGCCTCA GCTATTTATC GATGTTTCCT AAAGGATCCA ATATTCGTAT GGAAAATGTT 
ATTACACAGT GGGCTGCACT TGGCATCCTT GGATCAACAC ATGGATCATT GCCTGTTTAT 
GCCCAGGGGA GGAAGTATAT CCAGGAACTG CTTTCTGTGT TCTTCCTTGA AGCACATGAT 
AAATCCACAG ATTTTGGAAT TAATCATGCT AGTGCCTCAA AAGTCTTTAA AATGCACAGT 
TTAGTTCATG AGTTTGCCAG ATATGTTACC AGTCATGATT TATTTATTCT GGATGGTGAA 
AGGATGCGTA ACGATAACCC AGGGAATCGT ACTTCTGGAT ATGCACTACT GACTAGGTGC 
AGTGATAAAT CCAAAGTTAG CAGAGGTTTC CTGAAAAGTG TAAGGGCAAT ATGTCTGAAG 
GACTGCAGGG GAACAAAGCT TATTGAGAAA ATATTCTCTG CTTTAAAGCA TCTACGTGTG 
TTGGACCTTA GTAGATGCTC ATTTCTGGAG TTACCTAGTT CTATTTGCCA GTTAACCCAT 
CTAAGGTACA TTGATATTTC TTGTTCGGCT ATTCAATCAC TACCTGATCA AATGAGTTCT 
GTACAACACC TTGAAGCACT GGATCTCTCG GGGACATGTA TTCAAGTCCT ACCTGATTTT 
GTTAGAACAT TTAAGAAGCT AACATATCTT AACCTACAAG AATGCTGGGA ACTCCGTCAC 
TTGCCCTCAA AGCTTGATGA TATTAAGAGT TTGCAGCATC TTAACCTGTC ATGCTGTCCT 
GCAGCTCACC AGCTAGTTGA ATCAATCAGT GGATTTCAAG AACTTCGGTT TTTGGATATA 
TCAAGTTGCA CAGAACTTCA AACATTGCCA GAATCCTTCG TCAGACTAAC AAATTTAGAG 
GACCTAATCC TGTCCAAATG CACCAGGCTC AAGAAGCTAC CAGAATCATT TGGTGATAAA 
CTCTGTTTTC TTCGGTTCTT GAACATATCA TATTGCTGTG AGCTAGAAGA AGTGCCTGCA 
TCTCTCGGAA GGCTTGCTAG TTTGGAGGTT CTTATACTTT CAGGGTGCAA CAGAATTCAG 
AATCTCCCTC AATCATTCAG TGACATTGCA TTCTTACGGA TGCTAGACCT TTCAGGGTGT 
GCAGATCTTC ACATGGACCT TGGAATGCTA CCAAATAATA ATTTGGAGAA TCTTAATGTG 
GATGGATGTT GTAAGGTCTA TGCTATGCCT GGGTGGACTG TGAACTTCCC TAAACTACTT 
CCTGAATGTC TACAAACTTG TGACCAACAT GTTCGACGCT TAATTGAACA CAAGCAAGCA 
TGTCCCAATC ATAGTGAATT AGAGATCAGC AATGAACTAA ATGTGCTTGA CGAAACTGGG 
GAAGCAATGG CAAGTCAATC ATTTCAACAT GCTGGTGACT CTGAATGTAC CACCAAGAAA 
GTCATTGAGG ACTCATCTGC GCATACAAGT CCTAAAATTC CTCTGCATGG CACTACCCCT 
ACTGGAGTCC AGCTGAAATC AGCTGGTTCG TTTTTTGGGG CTTCTACAAT TCGATTTTCG 
AAAATGTCTC GTTGTTTTAT GTGTACAACA GACTTGAAAA TGAATAGTCA AGGAGAACAC 
ATGGACATGG GAAGGCTTAA AGATGGAATG GCTGTCGCAG TAAAGTTGCT CCATGATTCA 
AAGCAGGGCA GGCGAGAGTT TTTAGCTGAA CTCACAGCAA TTTCTGGCAT TGTGCATGAA 
AACCTAATCA CCTTAGTTGG ATGCTGTTGT GAAAGGTCCC ATAGAATCCT TGTATACAAT 
TATCTTGAGA ACAAAAGCCT TGCAGACAAA CTGTTAGGAT CAAGCAGTAG CACCATCCAA 
CTCAGCTGGA GAGCTCGTGT AAAAATTGCT ATAGGTGTTG CTCGTGGGCT TGCTTTTCTT 
CACGAAGAAA TCCGCCCCTC CATAATTCAC CGGGACATAA AAGCGAGCCA TATTCTTCTT 
GACAAGGATT TCACCCCAAA AGTTTCTGGT TTTGGGTTAA CAAGGCTCCT CCCACCAATT 
ACAACTTCTA TGGCCACTCG AGTTGCAGGA ACAATAGGAT ACATGGCTCC TGAATATGCA 
ATCAGGGGAC AAGTGACAAA GAAGGCGGAT ATCTTTAGTT TTGGTATTGT GCTACTGGAG 
ATTGTTAGTG GCAAATGTAA TCACGATAGT AGATTACCCC AGGAAGACCA ATTTCTGCTT 
AAAAGGACGT GGAGATCCTC TGAGGAAGGA AATCTGGTGG AAATCATAGA TAAAGATTTA 
GGGGACGACC TCGATGTTGA GGAGGCATGC CGATTCTTGA ATGTTGGTCT ACTATGTACT 
CAAAACGAGA TGGCGAGGCG TCCCAACATG AGGAACATCG TAAAGATGCT GACTGGGGAG 
AAAGGAGTCT CCGTGGTCAA GGTGACAAGA CCAATAATGC TGTGGTCTGA CTTGCTGGAA 
TTAAAAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021308595.1
CDS	194..3925
Translation	MATVSADIDRLQRRLASDGQRIKLPSSIRTDLSRMTRALYRLQGILTNVEKQPFDGSREPCLSKIKQNIYDVEDILDELENGSFRAQRSSRGWKLAEASDLWSQVAFSFSSNHSVLHCRMMNKMKKIRKSLDHASEDSFIFTLLHHRADADQSDHNVFDENAIIGRNKDKENVRVLLLSNSEQKISIIPIVGLGGLGKTALAQLIFSDQDERYNFDLRVWINLNMSYDFNSIASAIISEVNITEEGTSQVNGGEYNPQILMNCLLEVLNDKRCLIVLDGLWSIDEGQLLHLKRMLQSTQNTKIIVTTCSENVANLMHTVRPYKLDPLSEENCWTIFSQRVFGGGDNSDLTRIGKQIVNKCEGIPGVIHSLGAFMHDNGTSTWNWNDKNEELWKLEKQFPLQINLFSSVKQIYYKMPLALKSCLSYLSMFPKGSNIRMENVITQWAALGILGSTHGSLPVYAQGRKYIQELLSVFFLEAHDKSTDFGINHASASKVFKMHSLVHEFARYVTSHDLFILDGERMRNDNPGNRTSGYALLTRCSDKSKVSRGFLKSVRAICLKDCRGTKLIEKIFSALKHLRVLDLSRCSFLELPSSICQLTHLRYIDISCSAIQSLPDQMSSVQHLEALDLSGTCIQVLPDFVRTFKKLTYLNLQECWELRHLPSKLDDIKSLQHLNLSCCPAAHQLVESISGFQELRFLDISSCTELQTLPESFVRLTNLEDLILSKCTRLKKLPESFGDKLCFLRFLNISYCCELEEVPASLGRLASLEVLILSGCNRIQNLPQSFSDIAFLRMLDLSGCADLHMDLGMLPNNNLENLNVDGCCKVYAMPGWTVNFPKLLPECLQTCDQHVRRLIEHKQACPNHSELEISNELNVLDETGEAMASQSFQHAGDSECTTKKVIEDSSAHTSPKIPLHGTTPTGVQLKSAGSFFGASTIRFSKMSRCFMCTTDLKMNSQGEHMDMGRLKDGMAVAVKLLHDSKQGRREFLAELTAISGIVHENLITLVGCCCERSHRILVYNYLENKSLADKLLGSSSSTIQLSWRARVKIAIGVARGLAFLHEEIRPSIIHRDIKASHILLDKDFTPKVSGFGLTRLLPPITTSMATRVAGTIGYMAPEYAIRGQVTKKADIFSFGIVLLEIVSGKCNHDSRLPQEDQFLLKRTWRSSEEGNLVEIIDKDLGDDLDVEEACRFLNVGLLCTQNEMARRPNMRNIVKMLTGEKGVSVVKVTRPIMLWSDLLELKT

Target ORF information:

RefSeq Version	XM_021452920.1
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor putative disease resistance protein RGA3 (LOC8067274), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021452920.1

ORF Insert Sequence:

ATGGCCACTG TTAGCGCTGA TATAGATCGA CTCCAAAGGA GGCTTGCCTC CGACGGCCAA 
CGAATCAAGC TTCCCTCCAG CATACGGACG GATCTATCGC GCATGACAAG AGCTCTCTAC 
AGGTTGCAAG GCATCCTGAC TAATGTGGAG AAGCAACCAT TTGACGGGAG CAGAGAACCC 
TGCTTGAGCA AGATCAAACA GAATATTTAC GATGTGGAAG ATATACTAGA CGAGCTTGAA 
AATGGAAGCT TCAGGGCCCA ACGGAGCAGC AGGGGTTGGA AGCTAGCCGA GGCTTCAGAT 
CTCTGGTCAC AGGTAGCATT TTCCTTCTCA TCTAACCACT CAGTTCTGCA TTGTCGTATG 
ATGAATAAAA TGAAGAAGAT CAGAAAAAGT TTGGATCATG CATCAGAAGA CTCCTTCATA 
TTCACCTTAC TGCACCACAG AGCAGATGCA GATCAATCAG ATCACAATGT GTTTGATGAA 
AATGCAATCA TTGGAAGAAA TAAGGATAAG GAAAATGTAA GAGTCTTGCT CTTGAGTAAC 
AGTGAACAAA AAATTTCTAT TATTCCTATA GTTGGTCTTG GGGGATTGGG GAAAACAGCT 
CTTGCACAAC TCATTTTCAG TGACCAAGAT GAACGATATA ATTTTGACCT TCGTGTATGG 
ATCAATCTGA ACATGAGTTA TGATTTTAAC AGTATTGCTA GTGCTATAAT CTCAGAAGTT 
AACATAACAG AGGAAGGTAC CTCACAAGTT AATGGGGGTG AATATAATCC TCAGATACTA 
ATGAATTGTC TTCTAGAAGT ACTTAATGAT AAAAGATGTC TGATTGTGTT GGATGGCCTA 
TGGAGCATAG ATGAAGGGCA ATTACTTCAT CTCAAGAGAA TGCTACAGAG CACACAGAAT 
ACCAAGATTA TAGTGACTAC TTGTAGTGAA AATGTTGCAA ATCTGATGCA TACTGTTCGT 
CCCTACAAAT TGGATCCTTT ATCTGAAGAA AATTGTTGGA CAATATTTTC TCAAAGGGTA 
TTTGGTGGTG GAGATAACTC AGACCTCACA CGAATTGGGA AGCAGATCGT GAACAAGTGT 
GAAGGTATAC CGGGAGTTAT TCATTCTCTT GGTGCATTCA TGCACGATAA CGGAACGAGC 
ACTTGGAATT GGAATGACAA AAATGAAGAG CTGTGGAAAT TGGAGAAACA GTTTCCATTG 
CAGATCAACT TGTTCTCATC AGTCAAACAG ATATATTACA AGATGCCATT GGCATTGAAA 
TCGTGCCTCA GCTATTTATC GATGTTTCCT AAAGGATCCA ATATTCGTAT GGAAAATGTT 
ATTACACAGT GGGCTGCACT TGGCATCCTT GGATCAACAC ATGGATCATT GCCTGTTTAT 
GCCCAGGGGA GGAAGTATAT CCAGGAACTG CTTTCTGTGT TCTTCCTTGA AGCACATGAT 
AAATCCACAG ATTTTGGAAT TAATCATGCT AGTGCCTCAA AAGTCTTTAA AATGCACAGT 
TTAGTTCATG AGTTTGCCAG ATATGTTACC AGTCATGATT TATTTATTCT GGATGGTGAA 
AGGATGCGTA ACGATAACCC AGGGAATCGT ACTTCTGGAT ATGCACTACT GACTAGGTGC 
AGTGATAAAT CCAAAGTTAG CAGAGGTTTC CTGAAAAGTG TAAGGGCAAT ATGTCTGAAG 
GACTGCAGGG GAACAAAGCT TATTGAGAAA ATATTCTCTG CTTTAAAGCA TCTACGTGTG 
TTGGACCTTA GTAGATGCTC ATTTCTGGAG TTACCTAGTT CTATTTGCCA GTTAACCCAT 
CTAAGGTACA TTGATATTTC TTGTTCGGCT ATTCAATCAC TACCTGATCA AATGAGTTCT 
GTACAACACC TTGAAGCACT GGATCTCTCG GGGACATGTA TTCAAGTCCT ACCTGATTTT 
GTTAGAACAT TTAAGAAGCT AACATATCTT AACCTACAAG AATGCTGGGA ACTCCGTCAC 
TTGCCCTCAA AGCTTGATGA TATTAAGAGT TTGCAGCATC TTAACCTGTC ATGCTGTCCT 
GCAGCTCACC AGCTAGTTGA ATCAATCAGT GGATTTCAAG AACTTCGGTT TTTGGATATA 
TCAAGTTGCA CAGAACTTCA AACATTGCCA GAATCCTTCG TCAGACTAAC AAATTTAGAG 
GACCTAATCC TGTCCAAATG CACCAGGCTC AAGAAGCTAC CAGAATCATT TGGTGATAAA 
CTCTGTTTTC TTCGGTTCTT GAACATATCA TATTGCTGTG AGCTAGAAGA AGTGCCTGCA 
TCTCTCGGAA GGCTTGCTAG TTTGGAGGTT CTTATACTTT CAGGGTGCAA CAGAATTCAG 
AATCTCCCTC AATCATTCAG TGACATTGCA TTCTTACGGA TGCTAGACCT TTCAGGGTGT 
GCAGATCTTC ACATGGACCT TGGAATGCTA CCAAATAATA ATTTGGAGAA TCTTAATGTG 
GATGGATGTT GTAAGGTCTA TGCTATGCCT GGGTGGACTG TGAACTTCCC TAAACTACTT 
CCTGAATGTC TACAAACTTG TGACCAACAT GTTCGACGCT TAATTGAACA CAAGCAAGCA 
TGTCCCAATC ATAGTGAATT AGAGATCAGC AATGAACTAA ATGTGCTTGA CGAAACTGGG 
GAAGCAATGG CAAGTCAATC ATTTCAACAT GCTGGTGACT CTGAATGTAC CACCAAGAAA 
GTCATTGAGG ACTCATCTGC GCATACAAGT CCTAAAATTC CTCTGCATGG CACTACCCCT 
ACTGGAGTCC AGCTGAAATC AGCTGGTTCG TTTTTTGGGG CTTCTACAAT TCGATTTTCG 
AAAATGTCTC GTTGTTTTAT GTGTACAACA GACTTGAAAA TGAATAGTCA AGGAGAACAC 
ATGGACATGG GAAGGCTTAA AGATGGAATG GCTGTCGCAG TAAAGTTGCT CCATGATTCA 
AAGCAGGGCA GGCGAGAGTT TTTAGCTGAA CTCACAGCAA TTTCTGGCAT TGTGCATGAA 
AACCTAATCA CCTTAGTTGG ATGCTGTTGT GAAAGGTCCC ATAGAATCCT TGTATACAAT 
TATCTTGAGA ACAAAAGCCT TGCAGACAAA CTGTTAGGAT CAAGCAGTAG CACCATCCAA 
CTCAGCTGGA GAGCTCGTGT AAAAATTGCT ATAGGTGTTG CTCGTGGGCT TGCTTTTCTT 
CACGAAGAAA TCCGCCCCTC CATAATTCAC CGGGACATAA AAGCGAGCCA TATTCTTCTT 
GACAAGGATT TCACCCCAAA AGTTTCTGGT TTTGGGTTAA CAAGGCTCCT CCCACCAATT 
ACAACTTCTA TGGCCACTCG AGTTGCAGGA ACAATAGGAT ACATGGCTCC TGAATATGCA 
ATCAGGGGAC AAGTGACAAA GAAGGCGGAT ATCTTTAGTT TTGGTATTGT GCTACTGGAG 
ATTGTTAGTG GCAAATGTAA TCACGATAGT AGATTACCCC AGGAAGACCA ATTTCTGCTT 
AAAAGGACGT GGAGATCCTC TGAGGAAGGA AATCTGGTGG AAATCATAGA TAAAGATTTA 
GGGGACGACC TCGATGTTGA GGAGGCATGC CGATTCTTGA ATGTTGGTCT ACTATGTACT 
CAAAACGAGA TGGCGAGGCG TCCCAACATG AGGAACATCG TAAAGATGCT GACTGGGGAG 
AAAGGAGTCT CCGTGGTCAA GGTGACAAGA CCAATAATGC TGTGGTCTGA CTTGCTGGAA 
TTAAAAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Sorghum bicolor genome and the diversification of grasses.
Nature457(7229)551-6(2009 Jan)
Paterson AH,Bowers JE,Bruggmann R,Dubchak I,Grimwood J,Gundlach H,Haberer G,Hellsten U,Mitros T,Poliakov A,Schmutz J,Spannagl M,Tang H,Wang X,Wicker T,Bharti AK,Chapman J,Feltus FA,Gowik U,Grigoriev IV,Lyons E,Maher CA,Martis M,Narechania A,Otillar RP,Penning BW,Salamov AA,Wang Y,Zhang L,Carpita NC,Freeling M,Gingle AR,Hash CT,Keller B,Klein P,Kresovich S,McCann MC,Ming R,Peterson DG,Mehboob-ur-Rahman,Ware D,Westhoff P,Mayer KF,Messing J,Rokhsar DS

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LOC8067274 cDNA ORF clone, Sorghum bicolor(sorghum)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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