SORBIDRAFT_03G031700 cDNA ORF clone, Sorghum bicolor(Sorghum)

Gene
Clones
gRNAs

Summary

Gene Symbol	SORBIDRAFT_03G031700
Entrez Gene ID	8063089
Full Name	LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4
Gene Type	protein-coding
Organism	Sorghum bicolor(Sorghum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002456133.2	XP_002456178.2	LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4
XM_002456133.1	XP_002456178.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Sorghum bicolor genome and the diversification of grasses.
Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang H, Wang X, Wicker T, Bharti AK, Chapman J, Feltus FA, Gowik U, Grigoriev IV, Lyons E, Maher CA, Martis M, Narechania A, Otillar RP, Penning BW, Salamov AA, Wang Y, Zhang L, Carpita NC, Freeling M, Gingle AR, Hash CT, Keller B, Klein P, Kresovich S, McCann MC, Ming R, Peterson DG, Mehboob-ur-Rahman, Ware D, Westhoff P, Mayer KF, Messing J, Rokhsar DS
Nature457(7229)551-6(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SORBIDRAFT_03G031700 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SORBIDRAFT_03G031700 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc15637	XM_002456133.1 Latest version!	Sorghum bicolor hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OSc115474	XM_002456133.2 Latest version!	Sorghum bicolor LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 (LOC8063089), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SORBIDRAFT_03G031700 ( XM_002456133.1 ) cDNA ORF clone, Sorghum bicolor(Sorghum) -> XP_002456178.1 Sorghum bicolor hypothetical protein, mRNA.

Price & Availability↑

CloneID	OSc15637
Clone ID Related Accession (Same CDS sequence)	XM_002456133.1
Accession Version	XM_002456133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1247328000000
Organism	Sorghum bicolor(Sorghum)
Product	hypothetical protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012872). COMPLETENESS: incomplete on both ends.

ATGACGCGTC GTCGCTTCTT CTTTCTTGTA GTTGTAGGAG CCTGCCTGAT CCATGGCTTC 
GGCACGCCGC GTGGGTGCTC TGCTGCGCCG TCCTCCGGTT CCTCCTCGTG CTCGGCTGCC 
GTCATCACGT GCGGCAAGGT CAACATCACC TACCCGTTCC ATCTCCTCTC CGACGGCGGC 
GGCGCCTTCT GTGGCTACCC CGGCCTGGGC CTCGACTGCG TGGACGGCAA CACGACGGTG 
CTCCGGCTGA GCACCAACAA CAACACCGTG CTCACCATCA ACTACCCCGC GCGCACCATG 
TTCGTGGTCG ACTCCGGCAT CGCCCGCGCG GGGTACGACT GCCCGAGCGT CGTCCACAAC 
GTGACGGTCG CGCCGGGGTC TGGGGTGCAG TTCACCAAGT CGGACGCCAG CCTCACCTTC 
CTCGTCGGCT GCGCGGGCGG CGGCGGTGCC GCCCCGCCGT CGACGTGCCT CGACGGCTCC 
GCACCGATAG GCTGCGGCCT CAGCGGCGTG GCCGGGGACG GGGAGAGATC GTACGTCTTC 
CGCGGCGCCA CCGCCACCGC TGCCGCGCTG CCGCCGGGAG GAAGCGAGTG CGCCAGGTCC 
TGCCGTGGCA CGGTGACCGT GCCGGTGTAC GCGGCGCTGT TCGACGTGCC GAGCTTGTCC 
AACCTGTCCA CCAGCTACGC CAGGGTGCTC AAGAACGGGT TCGAGTTGAG CTGGGACAGG 
AGCTTCGACG CGCAGTGTGA CCCGTGCGAA AGCTCCGGTG GGTGGTGCAG CTACAACAGG 
ATGAGCACGG CTTCCGGAGG CGGCGGCCCG TTGGCGTTCA GTTGCATCTG CCCCGACGGA 
CGGTCGACGC CAACTGACTG CGGGCATGGT ATCACAAGGA GGAAAATCAG AACATATGTA 
ATAGCATCAA CAACGAGTAT GGCTTTCGTG CTCCTATTGT TTCTGGCCTA TCTCATGCAT 
CACAAGAGAA CCTATGGCTC ATTCGTCTTC TGGAGAAAAC GATCACACAT TTCTCCAAGA 
GTTGAAGCCT TCCTTCAAAG ATATGGTTCT CTCCACCCAA AGATTTACTC CTATATGGAA 
GTGAAACGGA TGACTAAATC ATTCGCGCAT CAACTAGGGC GAGGTGGATG TGGTGTTGTC 
TACAAGGGCA GTCTCCCTGA TGGCCGCTTA GTTGCTGTGA AAATGCTAAA GGAGTTGAAA 
GGGGATGATG AGCAATTCAT GAATGAGGTA GCAAGCATTA GCACCACATC TCATGTCAAC 
ATCGTGACTC TGCTAGGGTT TTGCGTACAA GGTTCCAAGA GAGCTCTTGT GTACGATTAC 
ATGACAAATG GTTCACTTGA GAGATTTATC TTCAGCAGGC ACTTAGAGGA CAAGAATTCT 
CTTAGTTGGG GCAAACTATT CGAGATTGTG GTCGGCATTG CAAGAGGACT AGAATATCTA 
CATAGGGGAT GCAAGACTAG AATTGTGCAT TTCGACATCA AGCCCCACAA TATCCTGCTT 
GATGAGAACT TTTGTCCGAA GATATCTGAC TTTGGATTGG CGAAATTAAG TGTACAAAAG 
GAGAGTGCAA TCTCCATTGG TGTAGCGAGG GGCACAATTG GGTACATCGC ACCGGAGGTT 
TTCTCTCGAC AATTTGGAGT TGTCACTAGC AAATCTGACG TCTATAGCTA TGGGATGATG 
ATTCTTGAGA TGGTTGGGTC TACAAGAACC ACAAATAATA ATACTAATTG TGAGAGCACC 
GATGAGCTCT ATTTTCCTCT GTGGATATAT GATAATTTGG ATCAGTACTG CCTTGATGCT 
TCTGAAACGT CTAAGGATGA CGGTGAGGTT GTAAGGAAGA TGATTGTTGT TGGGTTGTGG 
TGTGTACAAG TGATGCCCAT CGACAGGCCT TCAATGAGTC AGGTAGTTGA GATGTTGGAG 
AGTGACCTAA AAGACCTTCA GTTGCCGTCG AAGCCTCTTA CCCTTTCTTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002456178.1
CDS	1..1974
Translation	MTRRRFFFLVVVGACLIHGFGTPRGCSAAPSSGSSSCSAAVITCGKVNITYPFHLLSDGGGAFCGYPGLGLDCVDGNTTVLRLSTNNNTVLTINYPARTMFVVDSGIARAGYDCPSVVHNVTVAPGSGVQFTKSDASLTFLVGCAGGGGAAPPSTCLDGSAPIGCGLSGVAGDGERSYVFRGATATAAALPPGGSECARSCRGTVTVPVYAALFDVPSLSNLSTSYARVLKNGFELSWDRSFDAQCDPCESSGGWCSYNRMSTASGGGGPLAFSCICPDGRSTPTDCGHGITRRKIRTYVIASTTSMAFVLLLFLAYLMHHKRTYGSFVFWRKRSHISPRVEAFLQRYGSLHPKIYSYMEVKRMTKSFAHQLGRGGCGVVYKGSLPDGRLVAVKMLKELKGDDEQFMNEVASISTTSHVNIVTLLGFCVQGSKRALVYDYMTNGSLERFIFSRHLEDKNSLSWGKLFEIVVGIARGLEYLHRGCKTRIVHFDIKPHNILLDENFCPKISDFGLAKLSVQKESAISIGVARGTIGYIAPEVFSRQFGVVTSKSDVYSYGMMILEMVGSTRTTNNNTNCESTDELYFPLWIYDNLDQYCLDASETSKDDGEVVRKMIVVGLWCVQVMPIDRPSMSQVVEMLESDLKDLQLPSKPLTLSS

Target ORF information:

RefSeq Version	XM_002456133.1
Organism	Sorghum bicolor(Sorghum)
Definition	Sorghum bicolor hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002456133.1

ORF Insert Sequence:

ATGACGCGTC GTCGCTTCTT CTTTCTTGTA GTTGTAGGAG CCTGCCTGAT CCATGGCTTC 
GGCACGCCGC GTGGGTGCTC TGCTGCGCCG TCCTCCGGTT CCTCCTCGTG CTCGGCTGCC 
GTCATCACGT GCGGCAAGGT CAACATCACC TACCCGTTCC ATCTCCTCTC CGACGGCGGC 
GGCGCCTTCT GTGGCTACCC CGGCCTGGGC CTCGACTGCG TGGACGGCAA CACGACGGTG 
CTCCGGCTGA GCACCAACAA CAACACCGTG CTCACCATCA ACTACCCCGC GCGCACCATG 
TTCGTGGTCG ACTCCGGCAT CGCCCGCGCG GGGTACGACT GCCCGAGCGT CGTCCACAAC 
GTGACGGTCG CGCCGGGGTC TGGGGTGCAG TTCACCAAGT CGGACGCCAG CCTCACCTTC 
CTCGTCGGCT GCGCGGGCGG CGGCGGTGCC GCCCCGCCGT CGACGTGCCT CGACGGCTCC 
GCACCGATAG GCTGCGGCCT CAGCGGCGTG GCCGGGGACG GGGAGAGATC GTACGTCTTC 
CGCGGCGCCA CCGCCACCGC TGCCGCGCTG CCGCCGGGAG GAAGCGAGTG CGCCAGGTCC 
TGCCGTGGCA CGGTGACCGT GCCGGTGTAC GCGGCGCTGT TCGACGTGCC GAGCTTGTCC 
AACCTGTCCA CCAGCTACGC CAGGGTGCTC AAGAACGGGT TCGAGTTGAG CTGGGACAGG 
AGCTTCGACG CGCAGTGTGA CCCGTGCGAA AGCTCCGGTG GGTGGTGCAG CTACAACAGG 
ATGAGCACGG CTTCCGGAGG CGGCGGCCCG TTGGCGTTCA GTTGCATCTG CCCCGACGGA 
CGGTCGACGC CAACTGACTG CGGGCATGGT ATCACAAGGA GGAAAATCAG AACATATGTA 
ATAGCATCAA CAACGAGTAT GGCTTTCGTG CTCCTATTGT TTCTGGCCTA TCTCATGCAT 
CACAAGAGAA CCTATGGCTC ATTCGTCTTC TGGAGAAAAC GATCACACAT TTCTCCAAGA 
GTTGAAGCCT TCCTTCAAAG ATATGGTTCT CTCCACCCAA AGATTTACTC CTATATGGAA 
GTGAAACGGA TGACTAAATC ATTCGCGCAT CAACTAGGGC GAGGTGGATG TGGTGTTGTC 
TACAAGGGCA GTCTCCCTGA TGGCCGCTTA GTTGCTGTGA AAATGCTAAA GGAGTTGAAA 
GGGGATGATG AGCAATTCAT GAATGAGGTA GCAAGCATTA GCACCACATC TCATGTCAAC 
ATCGTGACTC TGCTAGGGTT TTGCGTACAA GGTTCCAAGA GAGCTCTTGT GTACGATTAC 
ATGACAAATG GTTCACTTGA GAGATTTATC TTCAGCAGGC ACTTAGAGGA CAAGAATTCT 
CTTAGTTGGG GCAAACTATT CGAGATTGTG GTCGGCATTG CAAGAGGACT AGAATATCTA 
CATAGGGGAT GCAAGACTAG AATTGTGCAT TTCGACATCA AGCCCCACAA TATCCTGCTT 
GATGAGAACT TTTGTCCGAA GATATCTGAC TTTGGATTGG CGAAATTAAG TGTACAAAAG 
GAGAGTGCAA TCTCCATTGG TGTAGCGAGG GGCACAATTG GGTACATCGC ACCGGAGGTT 
TTCTCTCGAC AATTTGGAGT TGTCACTAGC AAATCTGACG TCTATAGCTA TGGGATGATG 
ATTCTTGAGA TGGTTGGGTC TACAAGAACC ACAAATAATA ATACTAATTG TGAGAGCACC 
GATGAGCTCT ATTTTCCTCT GTGGATATAT GATAATTTGG ATCAGTACTG CCTTGATGCT 
TCTGAAACGT CTAAGGATGA CGGTGAGGTT GTAAGGAAGA TGATTGTTGT TGGGTTGTGG 
TGTGTACAAG TGATGCCCAT CGACAGGCCT TCAATGAGTC AGGTAGTTGA GATGTTGGAG 
AGTGACCTAA AAGACCTTCA GTTGCCGTCG AAGCCTCTTA CCCTTTCTTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SORBIDRAFT_03G031700 ( XM_002456133.2 ) cDNA ORF clone, Sorghum bicolor(Sorghum) -> XP_002456178.2 Sorghum bicolor LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 (LOC8063089), mRNA.

Price & Availability↑

CloneID	OSc115474
Clone ID Related Accession (Same CDS sequence)	XM_002456133.2
Accession Version	XM_002456133.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012872.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 13, 2017 this sequence version replaced XM_002456133.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases ##RefSeq-Attributes-END##

ATGGGATATA AAATGTATAT TATTGCAAGC ACATTGTCCC TTCTACTTCT GTGTCTACTT 
ATCCCCGCAC TATTCCTGGC TCGCAAATAT GGTTTGCTCT GCATTAAACG GAAGGAAGAA 
CCAATGATCA AGTCCTTTCT GCAAAAGAAT GGAAACCTAC ATACAAAAAG ATACACTTAT 
GCAGAAGTAA AAAGAATGAC AATGTCTTTT TCTGAAAAGC TAGGACAAGG TGGTTTTGGT 
GATGTATACA GAGGCAGCCT CTCGGACGGC GGTCAGGTAG CAGTCAAGAT GCTAAAGGAC 
CCCAAGGGTG ATGGGGAGGA ATTCATGAAT GAGGTGTCCA GCATTAGCAG AACTTCACAT 
GTCAATGTTG TTATGCTCAT AGGATTTTGC TTGGAAGGAT CCAAAAGGGC ACTCATCTAT 
GAGTACATGC CTAATGGTTC ACTTGAAAGG TATGCCTTCA ATAGCAACAT GAACAACCAA 
AATTCTCTAG GTTGGGAGAA ATTATTTGAC ATAGCAATTG GCATCGCTAG AGGACTTGAA 
TATCTCCACC GCGGATGCAA TACTCGCATA GTGCATTTTG ATATCAAACC CCACAACATT 
TTATTAGATC ATGATTTCTG TCCCAAGATT TCTGATTTTG GATTAGCCAA ACTATGCCCA 
AATAAAGAGA GTGCCATTTC CATTGCCGGT GCAAGAGGAA CAATAGGCTA CATTGCACCT 
GAGGTTTTCT CCAAGCAATT CGGAACAGTC AGTAGCAAGT CAGATGTCTA TAGTTATGGA 
ATGATGGTCC TTGAGATGGT TGGGGCAAGG GACAAAAATA TGAATGCAGA TAGTGAATCT 
AGCAGCCAAT ATTTCCCTCA GTGGATTTAT GAACATTTGG ATGACTACTG TATCAGTGCT 
TCTGAGGTTA ACAGTGAGAC CACAGAGCTC GTGAGAAAGA TGATAGTGGT AGGTTTGTGG 
TGTATACAAG CTATACCAAC CGATCGTCCA ACAATGACTA AAGTCGTTGA GATGCTAGAA 
GGAAGCACAA GTAATCTTGA GCTGCCACCA AAAGTTGCTA CTTGCTACAA GTACAGCTCT 
CTTCTCTGTG TTCTACGGAC TACGGAGTAC GGAGTACTAC TACTCTGCTA TTTCGTGATG 
ACGCGTCGTC GCTTCTTCTT TCTTGTAGTT GTAGGAGCCT GCCTGATCCA TGGCTTCGGC 
ACGCCGCGTG GGTGCTCTGC TGCGCCGTCC TCCGGTTCCT CCTCGTGCTC GGCTGCCGTC 
ATCACGTGCG GCAAGGTCAA CATCACCTAC CCGTTCCATC TCCTCTCCGA CGGCGGCGGC 
GCCTTCTGTG GCTACCCCGG CCTGGGCCTC GACTGCGTGG ACGGCAACAC GACGGTGCTC 
CGGCTGAGCA CCAACAACAA CACCGTGCTC ACCATCAACT ACCCCGCGCG CACCATGTTC 
GTGGTCGACT CCGGCATCGC CCGCGCGGGG TACGACTGCC CGAGCGTCGT CCACAACGTG 
ACGGTCGCGC CGGGGTCTGG GGTGCAGTTC ACCAAGTCGG ACGCCAGCCT CACCTTCCTC 
GTCGGCTGCG CGGGCGGCGG CGGTGCCGCC CCGCCGTCGA CGTGCCTCGA CGGCTCCGCA 
CCGATAGGCT GCGGCCTCAG CGGCGTGGCC GGGGACGGGG AGAGATCGTA CGTCTTCCGC 
GGCGCCACCG CCACCGCTGC CGCGCTGCCG CCGGGAGGAA GCGAGTGCGC CAGGTCCTGC 
CGTGGCACGG TGACCGTGCC GGTGTACGCG GCGCTGTTCG ACGTGCCGAG CTTGTCCAAC 
CTGTCCACCA GCTACGCCAG GGTGCTCAAG AACGGGTTCG AGTTGAGCTG GGACAGGAGC 
TTCGACGCGC AGTGTGACCC GTGCGAAAGC TCCGGTGGGT GGTGCAGCTA CAACAGGATG 
AGCACGGCTT CCGGAGGCGG CGGCCCGTTG GCGTTCAGTT GCATCTGCCC CGACGGACGG 
TCGACGCCAA CTGACTGCGG GCATGGTATC ACAAGGAGGA AAATCAGAAC ATATGTAATA 
GCATCAACAA CGAGTATGGC TTTCGTGCTC CTATTGTTTC TGGCCTATCT CATGCATCAC 
AAGAGAACCT ATGGCTCATT CGTCTTCTGG AGAAAACGAT CACACATTTC TCCAAGAGTT 
GAAGCCTTCC TTCAAAGATA TGGTTCTCTC CACCCAAAGA TTTACTCCTA TATGGAAGTG 
AAACGGATGA CTAAATCATT CGCGCATCAA CTAGGGCGAG GTGGATGTGG TGTTGTCTAC 
AAGGGCAGTC TCCCTGATGG CCGCTTAGTT GCTGTGAAAA TGCTAAAGGA GTTGAAAGGG 
GATGATGAGC AATTCATGAA TGAGGTAGCA AGCATTAGCA CCACATCTCA TGTCAACATC 
GTGACTCTGC TAGGGTTTTG CGTACAAGGT TCCAAGAGAG CTCTTGTGTA CGATTACATG 
ACAAATGGTT CACTTGAGAG ATTTATCTTC AGCAGGCACT TAGAGGACAA GAATTCTCTT 
AGTTGGGGCA AACTATTCGA GATTGTGGTC GGCATTGCAA GAGGACTAGA ATATCTACAT 
AGGGGATGCA AGACTAGAAT TGTGCATTTC GACATCAAGC CCCACAATAT CCTGCTTGAT 
GAGAACTTTT GTCCGAAGAT ATCTGACTTT GGATTGGCGA AATTAAGTGT ACAAAAGGAG 
AGTGCAATCT CCATTGGTGT AGCGAGGGGC ACAATTGGGT ACATCGCACC GGAGGTTTTC 
TCTCGACAAT TTGGAGTTGT CACTAGCAAA TCTGACGTCT ATAGCTATGG GATGATGATT 
CTTGAGATGG TTGGGTCTAC AAGAACCACA AATAATAATA CTAATTGTGA GAGCACCGAT 
GAGCTCTATT TTCCTCTGTG GATATATGAT AATTTGGATC AGTACTGCCT TGATGCTTCT 
GAAACGTCTA AGGATGACGG TGAGGTTGTA AGGAAGATGA TTGTTGTTGG GTTGTGGTGT 
GTACAAGTGA TGCCCATCGA CAGGCCTTCA ATGAGTCAGG TAGTTGAGAT GTTGGAGAGT 
GACCTAAAAG ACCTTCAGTT GCCGTCGAAG CCTCTTACCC TTTCTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002456178.2
CDS	243..3353
Translation	MGYKMYIIASTLSLLLLCLLIPALFLARKYGLLCIKRKEEPMIKSFLQKNGNLHTKRYTYAEVKRMTMSFSEKLGQGGFGDVYRGSLSDGGQVAVKMLKDPKGDGEEFMNEVSSISRTSHVNVVMLIGFCLEGSKRALIYEYMPNGSLERYAFNSNMNNQNSLGWEKLFDIAIGIARGLEYLHRGCNTRIVHFDIKPHNILLDHDFCPKISDFGLAKLCPNKESAISIAGARGTIGYIAPEVFSKQFGTVSSKSDVYSYGMMVLEMVGARDKNMNADSESSSQYFPQWIYEHLDDYCISASEVNSETTELVRKMIVVGLWCIQAIPTDRPTMTKVVEMLEGSTSNLELPPKVATCYKYSSLLCVLRTTEYGVLLLCYFVMTRRRFFFLVVVGACLIHGFGTPRGCSAAPSSGSSSCSAAVITCGKVNITYPFHLLSDGGGAFCGYPGLGLDCVDGNTTVLRLSTNNNTVLTINYPARTMFVVDSGIARAGYDCPSVVHNVTVAPGSGVQFTKSDASLTFLVGCAGGGGAAPPSTCLDGSAPIGCGLSGVAGDGERSYVFRGATATAAALPPGGSECARSCRGTVTVPVYAALFDVPSLSNLSTSYARVLKNGFELSWDRSFDAQCDPCESSGGWCSYNRMSTASGGGGPLAFSCICPDGRSTPTDCGHGITRRKIRTYVIASTTSMAFVLLLFLAYLMHHKRTYGSFVFWRKRSHISPRVEAFLQRYGSLHPKIYSYMEVKRMTKSFAHQLGRGGCGVVYKGSLPDGRLVAVKMLKELKGDDEQFMNEVASISTTSHVNIVTLLGFCVQGSKRALVYDYMTNGSLERFIFSRHLEDKNSLSWGKLFEIVVGIARGLEYLHRGCKTRIVHFDIKPHNILLDENFCPKISDFGLAKLSVQKESAISIGVARGTIGYIAPEVFSRQFGVVTSKSDVYSYGMMILEMVGSTRTTNNNTNCESTDELYFPLWIYDNLDQYCLDASETSKDDGEVVRKMIVVGLWCVQVMPIDRPSMSQVVEMLESDLKDLQLPSKPLTLSS

Target ORF information:

RefSeq Version	XM_002456133.2
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 (LOC8063089), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002456133.2

ORF Insert Sequence:

ATGGGATATA AAATGTATAT TATTGCAAGC ACATTGTCCC TTCTACTTCT GTGTCTACTT 
ATCCCCGCAC TATTCCTGGC TCGCAAATAT GGTTTGCTCT GCATTAAACG GAAGGAAGAA 
CCAATGATCA AGTCCTTTCT GCAAAAGAAT GGAAACCTAC ATACAAAAAG ATACACTTAT 
GCAGAAGTAA AAAGAATGAC AATGTCTTTT TCTGAAAAGC TAGGACAAGG TGGTTTTGGT 
GATGTATACA GAGGCAGCCT CTCGGACGGC GGTCAGGTAG CAGTCAAGAT GCTAAAGGAC 
CCCAAGGGTG ATGGGGAGGA ATTCATGAAT GAGGTGTCCA GCATTAGCAG AACTTCACAT 
GTCAATGTTG TTATGCTCAT AGGATTTTGC TTGGAAGGAT CCAAAAGGGC ACTCATCTAT 
GAGTACATGC CTAATGGTTC ACTTGAAAGG TATGCCTTCA ATAGCAACAT GAACAACCAA 
AATTCTCTAG GTTGGGAGAA ATTATTTGAC ATAGCAATTG GCATCGCTAG AGGACTTGAA 
TATCTCCACC GCGGATGCAA TACTCGCATA GTGCATTTTG ATATCAAACC CCACAACATT 
TTATTAGATC ATGATTTCTG TCCCAAGATT TCTGATTTTG GATTAGCCAA ACTATGCCCA 
AATAAAGAGA GTGCCATTTC CATTGCCGGT GCAAGAGGAA CAATAGGCTA CATTGCACCT 
GAGGTTTTCT CCAAGCAATT CGGAACAGTC AGTAGCAAGT CAGATGTCTA TAGTTATGGA 
ATGATGGTCC TTGAGATGGT TGGGGCAAGG GACAAAAATA TGAATGCAGA TAGTGAATCT 
AGCAGCCAAT ATTTCCCTCA GTGGATTTAT GAACATTTGG ATGACTACTG TATCAGTGCT 
TCTGAGGTTA ACAGTGAGAC CACAGAGCTC GTGAGAAAGA TGATAGTGGT AGGTTTGTGG 
TGTATACAAG CTATACCAAC CGATCGTCCA ACAATGACTA AAGTCGTTGA GATGCTAGAA 
GGAAGCACAA GTAATCTTGA GCTGCCACCA AAAGTTGCTA CTTGCTACAA GTACAGCTCT 
CTTCTCTGTG TTCTACGGAC TACGGAGTAC GGAGTACTAC TACTCTGCTA TTTCGTGATG 
ACGCGTCGTC GCTTCTTCTT TCTTGTAGTT GTAGGAGCCT GCCTGATCCA TGGCTTCGGC 
ACGCCGCGTG GGTGCTCTGC TGCGCCGTCC TCCGGTTCCT CCTCGTGCTC GGCTGCCGTC 
ATCACGTGCG GCAAGGTCAA CATCACCTAC CCGTTCCATC TCCTCTCCGA CGGCGGCGGC 
GCCTTCTGTG GCTACCCCGG CCTGGGCCTC GACTGCGTGG ACGGCAACAC GACGGTGCTC 
CGGCTGAGCA CCAACAACAA CACCGTGCTC ACCATCAACT ACCCCGCGCG CACCATGTTC 
GTGGTCGACT CCGGCATCGC CCGCGCGGGG TACGACTGCC CGAGCGTCGT CCACAACGTG 
ACGGTCGCGC CGGGGTCTGG GGTGCAGTTC ACCAAGTCGG ACGCCAGCCT CACCTTCCTC 
GTCGGCTGCG CGGGCGGCGG CGGTGCCGCC CCGCCGTCGA CGTGCCTCGA CGGCTCCGCA 
CCGATAGGCT GCGGCCTCAG CGGCGTGGCC GGGGACGGGG AGAGATCGTA CGTCTTCCGC 
GGCGCCACCG CCACCGCTGC CGCGCTGCCG CCGGGAGGAA GCGAGTGCGC CAGGTCCTGC 
CGTGGCACGG TGACCGTGCC GGTGTACGCG GCGCTGTTCG ACGTGCCGAG CTTGTCCAAC 
CTGTCCACCA GCTACGCCAG GGTGCTCAAG AACGGGTTCG AGTTGAGCTG GGACAGGAGC 
TTCGACGCGC AGTGTGACCC GTGCGAAAGC TCCGGTGGGT GGTGCAGCTA CAACAGGATG 
AGCACGGCTT CCGGAGGCGG CGGCCCGTTG GCGTTCAGTT GCATCTGCCC CGACGGACGG 
TCGACGCCAA CTGACTGCGG GCATGGTATC ACAAGGAGGA AAATCAGAAC ATATGTAATA 
GCATCAACAA CGAGTATGGC TTTCGTGCTC CTATTGTTTC TGGCCTATCT CATGCATCAC 
AAGAGAACCT ATGGCTCATT CGTCTTCTGG AGAAAACGAT CACACATTTC TCCAAGAGTT 
GAAGCCTTCC TTCAAAGATA TGGTTCTCTC CACCCAAAGA TTTACTCCTA TATGGAAGTG 
AAACGGATGA CTAAATCATT CGCGCATCAA CTAGGGCGAG GTGGATGTGG TGTTGTCTAC 
AAGGGCAGTC TCCCTGATGG CCGCTTAGTT GCTGTGAAAA TGCTAAAGGA GTTGAAAGGG 
GATGATGAGC AATTCATGAA TGAGGTAGCA AGCATTAGCA CCACATCTCA TGTCAACATC 
GTGACTCTGC TAGGGTTTTG CGTACAAGGT TCCAAGAGAG CTCTTGTGTA CGATTACATG 
ACAAATGGTT CACTTGAGAG ATTTATCTTC AGCAGGCACT TAGAGGACAA GAATTCTCTT 
AGTTGGGGCA AACTATTCGA GATTGTGGTC GGCATTGCAA GAGGACTAGA ATATCTACAT 
AGGGGATGCA AGACTAGAAT TGTGCATTTC GACATCAAGC CCCACAATAT CCTGCTTGAT 
GAGAACTTTT GTCCGAAGAT ATCTGACTTT GGATTGGCGA AATTAAGTGT ACAAAAGGAG 
AGTGCAATCT CCATTGGTGT AGCGAGGGGC ACAATTGGGT ACATCGCACC GGAGGTTTTC 
TCTCGACAAT TTGGAGTTGT CACTAGCAAA TCTGACGTCT ATAGCTATGG GATGATGATT 
CTTGAGATGG TTGGGTCTAC AAGAACCACA AATAATAATA CTAATTGTGA GAGCACCGAT 
GAGCTCTATT TTCCTCTGTG GATATATGAT AATTTGGATC AGTACTGCCT TGATGCTTCT 
GAAACGTCTA AGGATGACGG TGAGGTTGTA AGGAAGATGA TTGTTGTTGG GTTGTGGTGT 
GTACAAGTGA TGCCCATCGA CAGGCCTTCA ATGAGTCAGG TAGTTGAGAT GTTGGAGAGT 
GACCTAAAAG ACCTTCAGTT GCCGTCGAAG CCTCTTACCC TTTCTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Sorghum bicolor genome and the diversification of grasses.
Nature457(7229)551-6(2009 Jan)
Paterson AH,Bowers JE,Bruggmann R,Dubchak I,Grimwood J,Gundlach H,Haberer G,Hellsten U,Mitros T,Poliakov A,Schmutz J,Spannagl M,Tang H,Wang X,Wicker T,Bharti AK,Chapman J,Feltus FA,Gowik U,Grigoriev IV,Lyons E,Maher CA,Martis M,Narechania A,Otillar RP,Penning BW,Salamov AA,Wang Y,Zhang L,Carpita NC,Freeling M,Gingle AR,Hash CT,Keller B,Klein P,Kresovich S,McCann MC,Ming R,Peterson DG,Mehboob-ur-Rahman,Ware D,Westhoff P,Mayer KF,Messing J,Rokhsar DS

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SORBIDRAFT_03G031700 cDNA ORF clone, Sorghum bicolor(Sorghum)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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