LOC8061339 cDNA ORF clone, Sorghum bicolor(sorghum)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8061339
Entrez Gene ID	8061339
Full Name	anaphase-promoting complex subunit 1
Gene Type	protein-coding
Organism	Sorghum bicolor(sorghum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002439572.1	XP_002439617.1	hypothetical protein
XM_021447941.1	XP_021303616.1	anaphase-promoting complex subunit 1 isoform X1
XM_002439575.2	XP_002439620.2	anaphase-promoting complex subunit 1 isoform X3
XM_021447942.1	XP_021303617.1	anaphase-promoting complex subunit 1 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Sorghum bicolor genome and the diversification of grasses.
Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang H, Wang X, Wicker T, Bharti AK, Chapman J, Feltus FA, Gowik U, Grigoriev IV, Lyons E, Maher CA, Martis M, Narechania A, Otillar RP, Penning BW, Salamov AA, Wang Y, Zhang L, Carpita NC, Freeling M, Gingle AR, Hash CT, Keller B, Klein P, Kresovich S, McCann MC, Ming R, Peterson DG, Mehboob-ur-Rahman, Ware D, Westhoff P, Mayer KF, Messing J, Rokhsar DS
Nature457(7229)551-6(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC8061339 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8061339 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSc12791	XM_002439572.1 Latest version!	Sorghum bicolor hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OSc105246	XM_021447941.1 Latest version!	Sorghum bicolor anaphase-promoting complex subunit 1 (LOC8061339), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc105247	XM_021447942.1 Latest version!	Sorghum bicolor anaphase-promoting complex subunit 1 (LOC8061339), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc105248	XM_002439575.2 Latest version!	Sorghum bicolor anaphase-promoting complex subunit 1 (LOC8061339), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8061339 ( XM_002439572.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_002439617.1 Sorghum bicolor hypothetical protein, mRNA.

Price & Availability↑

CloneID	OSc12791
Clone ID Related Accession (Same CDS sequence)	XM_002439572.1
Accession Version	XM_002439572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1247328000000
Organism	Sorghum bicolor(Sorghum)
Product	hypothetical protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012878). COMPLETENESS: incomplete on both ends.

ATGGCTTCGG CGATCGGTTC GCGGCGGCTA ACGGTGCTGC GAGAGTTCCG TCCTCACGGG 
CTCGCAGTCG AGGAGGCCGA CGGCGAGGGC GCCCCCGGGG CGCCGCCGCC GCAGGATTAC 
GACTACTTCC TCTTTGACCC CGCCCTCGCC GTTTCCCCCA GCCCCGACAC CAGCAATGAG 
GCCTCCTCCT CCGGCGCAGA CGGCGACCAC GAGCTCTTCA TCCGTGGCAA TCAGATAATA 
TGGAGCAATG GTTCTCGAGT TCACAAAAGA TATGTATCAC CTAATACAGT CAAGATGGCT 
TGCTGGTGTA GGATGAATGC AATCAGCGAT GCTCTTCTAT GTGTCCTGCA AGTTGATACT 
TTATCTCTAT ACAATGTAAC TGGTGAAATT GTTAGTATTC CCCTACCTTA TGCTGTTTCA 
TCAATATGGC CACTCCCCTT TGGTCTTCTA CTTCAGAAAA TTCCCTTTTT TCCAAGTTTT 
AGGTCATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002439617.1
CDS	1..492
Translation	MASAIGSRRLTVLREFRPHGLAVEEADGEGAPGAPPPQDYDYFLFDPALAVSPSPDTSNEASSSGADGDHELFIRGNQIIWSNGSRVHKRYVSPNTVKMACWCRMNAISDALLCVLQVDTLSLYNVTGEIVSIPLPYAVSSIWPLPFGLLLQKIPFFPSFRSF

Target ORF information:

RefSeq Version	XM_002439572.1
Organism	Sorghum bicolor(Sorghum)
Definition	Sorghum bicolor hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002439572.1

ORF Insert Sequence:

ATGGCTTCGG CGATCGGTTC GCGGCGGCTA ACGGTGCTGC GAGAGTTCCG TCCTCACGGG 
CTCGCAGTCG AGGAGGCCGA CGGCGAGGGC GCCCCCGGGG CGCCGCCGCC GCAGGATTAC 
GACTACTTCC TCTTTGACCC CGCCCTCGCC GTTTCCCCCA GCCCCGACAC CAGCAATGAG 
GCCTCCTCCT CCGGCGCAGA CGGCGACCAC GAGCTCTTCA TCCGTGGCAA TCAGATAATA 
TGGAGCAATG GTTCTCGAGT TCACAAAAGA TATGTATCAC CTAATACAGT CAAGATGGCT 
TGCTGGTGTA GGATGAATGC AATCAGCGAT GCTCTTCTAT GTGTCCTGCA AGTTGATACT 
TTATCTCTAT ACAATGTAAC TGGTGAAATT GTTAGTATTC CCCTACCTTA TGCTGTTTCA 
TCAATATGGC CACTCCCCTT TGGTCTTCTA CTTCAGAAAA TTCCCTTTTT TCCAAGTTTT 
AGGTCATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8061339 ( XM_021447941.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_021303616.1 Sorghum bicolor anaphase-promoting complex subunit 1 (LOC8061339), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSc105246
Clone ID Related Accession (Same CDS sequence)	XM_021447941.1
Accession Version	XM_021447941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	anaphase-promoting complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012878.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CGATCGGTTC GCGGCGGCTA ACGGTGCTGC GAGAGTTCCG TCCTCACGGG 
CTCGCAGTCG AGGAGGCCGA CGGCGAGGGC GCCCCCGGGG CGCCGCCGCC GCAGGATTAC 
GACTACTTCC TCTTTGACCC CGCCCTCGCC GTTTCCCCCA GCCCCGACAC CAGCAATGAG 
GCCTCCTCCT CCGGCGCAGA CGGCGACCAC GAGCTCTTCA TCCGTGGCAA TCAGATAATA 
TGGAGCAATG GTTCTCGAGT TCACAAAAGA TATGTATCAC CTAATACAGT CAAGATGGCT 
TGCTGGTGTA GGATGAATGC AATCAGCGAT GCTCTTCTAT GTGTCCTGCA AGTTGATACT 
TTATCTCTAT ACAATGTAAC TGGTGAAATT GTTAGTATTC CCCTACCTTA TGCTGTTTCA 
TCAATATGGC CACTCCCCTT TGGTCTTCTA CTTCAGAAAT CAACTGATGG AGGTCATATG 
GTGTCATCCT CAAGTTCACT GCTAAATGCA AGGGATTTAA ATCGTCCTAG CAAGGAATAT 
GGATTGACCT ACAATGTTCC GTTCCAAGCA AACACATTGG AGACAGACAG CAAAGATAAT 
GGAGCCATTG TCTCATCACA CCTCATCTTG AAACATCCAC TTGAAGAGCC TCAGGCAACA 
TATGTTGAAG AAAAGGACAA ACTAACTATG ATGAGAGATT TTGATGAAGA AACTATCTGG 
ACAAGTGACA TGATACCTCT AATAGCATCA TATCATAAAG GGAAGTTTCA GCATTCTGTC 
TGGCAAATTG ATGGTGCTAG TTACCAGGAA GCAATGAATG AAAATACAAT GCTACCTGTC 
GATATTTCTT CACATAAATG TGCATTCCGC AAGATATGGC AAGGGGAATG CTCCGAGTCT 
GCTGCTAGTA AGGTTTTCTT GGCAACTGAT ATTGATGGGA TGCCAATAAT TAGTTTTTTA 
CTGGATGAGC AAAAAATATT GCTTGCTGTA AGGATTCAAG TTGATGATAC TAGTGAAGAA 
GCTTTGGGTG ATATTAAGCC TCACATGAGC TGGAACATTC CTGCATTTGC TGCTGTGCCA 
GTTGTCGTGA CTCGTCCAAG AGTACGGGTT GGTGTTCTTC CCTTTACAGA CATCCTTGTT 
CTGAGTTCAG ATAATGATCT ACTTCTCTAT TCTGGGAAGC AGTGCCTCTG CAAATATACT 
CTCCCTAATG AATTGGGAAA GGGTTTCTTT TCAAATCATG AGCTAAATTC AGATATTTCA 
GATATTTACT CTGATCTGAA GATCACAAGC ATAGCTGATG CTGTTGAAGG CCGTATAAAC 
GTGACATGCA GTAATGGCCT GATGCTCAGA TGTTCGTTGC GCAAAAATCC TTCCTCTTCT 
TTGGTTAGTG ACTGCATAAC TGCAATGGCA GAGGGTTTGC AGAGTTGCTT TTACAATCAG 
TTTGTTTCTC TTTTTTGGGG TGATAGTGAT GCTGCCTACC TACATTCAAG CTCTCAGGTG 
GATTCAGAAT GGGAATATTT TAGTCATGAA ATAAAAAGAG TTTGTACAAA GTATGGACAA 
ACTTTGTCAA CTAAATCTCT CACTTTGCCA AGTAAAGCTT GGGACTTCCT GATCAACAGC 
AAATACCATT CCCAATACTG CAAAAGGGCC CTATCCTCTT CCAATTCACT TTTGCCTGCG 
TCATATAATA CATGCAAAAC TGTCTTTAAT CCCTTTCTCC AGGATGAGCA TAGTTTAGAT 
GTGCCATATA ATATTCGGTT CATGAGAGAA ACATTGGAGA CATTGCATGC CCTATATGAG 
AACTTGAAGC TTAACATCTT GCGGAAAGAG GATCTAGGAC GTCTCGCTTC ATTGTTATGT 
ACTGTTGCTT CATCTCTGGG TGAACATAGT TATGTTGATT ATTATTGCCG TGACTTTCCG 
CTCAACTTGA TTGAATCCCC ATCCCTAGTC TCTTCAAATT CTTTAACAAC TCCGCCGTCC 
CTGTTTCGTT GGTTTGAATA CTGCCTATGT CATGGTTTGG ACTCGGCAAA GCTGGAAGAC 
ATTCCTACTC TAATGCGCAA ACAGAAAGTT TCTGCTGTGA GTTGGGGTAG GAAGGTGTTG 
TCATTTTATA GTTTGTTGTT AGGAGCAGAA AGAAAAGGCA AAAATCTCTC TTCTGGTGTG 
TATTGTGAAG TTGCCAGTGG TTCAGCCAGA AACACTGAGG AGCTTACTGT TCTGGCTATG 
GTTGCTGAAA AGTTTGGTCA TCAGCAATTG GATTTATTAC CAGTTGGTGT ATCTCTTGTG 
CTTCGCCATG CACTGGACAA ATGTCGTGAC TCTCCACCTG ATGATTGGCC TGCAACTGCT 
TATGTTCTTG TTGGCAGGGA AGACCTAGCT GCAGCAAAGA TGGGGACAGT TAGGAAAGAA 
AACCTGTTGT GGAACAATGA TAATTTGACA TCAATGTCAG TCCCTTATAT GCTACATCTG 
CAACCTGTAA TCATACCAAC AACTGCACCT GACATACCTG CATCAGAAGT TTTGAATTCT 
GATGATTCAG ATTCTGTCTA TAAATCTGTT GAGGATGGCA TGGAACATAT CTTCACTTCA 
ACAACACAAC TCCGATTTGG TCATGATTTG CGCTTGAATG AGGTTAGACG CCTCTTATGC 
TCAGCAAGGC CAGTGGCTAT ACAAACACCT ACTAATCCTA GCGTGTCGGA TCAAGACCTA 
CATCAGCAAC AATTATGGAA TTTTGCACAA AGGACAACTG CTTTACCTTT TGGGCGTGGG 
GCTTTTACTT TAGCTACAAC TTACACTTTG TTGACAGAGG TTCTGGTGTT CCCAAAGCTT 
GTGCTGGCTG GTCGTTTGCC TGCACAGCAG AATGCCACAG TAAATCTTGA CCTAAGTAGT 
AGAAGTGTCT CAGAATTCAA ATCTTGGGCT GAGTTTCATA ATGGTGTTGC TGCTGGCCTG 
AGGCTTGCCC CTTTTCAGGA GAAAATGTTG AGAACTTGGA TACAATACAA TAGACCTTCT 
GAACCAAATT TTACTCATGC TGGTTTGCTT CTAGCATTTG GCTTGCATGA GCATCTAAGA 
GTCTTAACTA TGACTGATGC ATATCGATAC CTCTCACAGG AGCATGATAT AACAACACTT 
GGTTTGTTAC TTGGTTTGGC AGCATCTCAT AGGGGAACAA TGGATCCTGC AATATCAAAG 
ATGCTCTACT TTCATGTTCC TTCTCGACAT CCATCTTCTA CTCCAGAGCT GGAGTTACCA 
ACTCTTCTAC AGTCAGCAGC AGTAATGGGA ATTGGACTTC TATATGAAGG CTCAGCTCAT 
GCTCTCACCA TGAAGATACT TCTGGGTGAG ATTGGCCGAA GAAGTGGAGG TGACAACGTG 
CTAGAAAGGG AAGGGTATGC CGTTGCTGCC GGTTCTGCAT TAGGATTTGT TGCCTTGGGT 
CATGGCAATT GTGCATTTGA ATTCATGGAT ACTTTCCTAG ATCGACTTTT TGAATATATT 
GGCAGTAAAG AAGTCTACCA TGAAAAGTAC CTAAATGCAA CAACTGCCGA TGATCAAAGT 
TGCAACCCTG GACAGATGAT GGATGGAACA CAAATAAATG TTGATGTTAC TGCACCTGGA 
GCAATAATTG CTCTAACCTT GATATTTTTG AAAGCCGAGT CTGAGGAGAT TGCAGCTAGA 
CTCAATATCC CCAATACTTA CTTTGATTTG CAGTATGTGA GGCCTGATTT TGTTATGCTT 
CGCATTATAG CCAGGAATTT AATATTGTGG AGCAGAATAC AACCTACCAA AGGATGGGTT 
GATTCTCAAA TTCCTGAAAC AGTCAAGTCT GGTGTTTCCA ACATGAGTGA AGGGACCATT 
GACAGTGATG AATTGGATGC TGAAGCTCTT TTTCAAGCTT ATGTTAACAT AGTGACTGGA 
GCATGTATTG CACTTGGACT CAAGTATGCT GGCAGCAGAA ATGGTGATGC CCAAGAGCTA 
CTCTACGCTT ACACTGTCCA TTTTCTAAAT GAGATTAAGC ACATACCTGT TGAAACATCA 
AATATACTAC CAAAGGGATT ACTACAATAC GTTGACCGTG GAACTCTTGA ACTCTGCTTG 
CATCTTATTG TTCTATCTCT CTCTCTGGTA ATGGCAGGAT CTGGAAATTT GCAGACTTTC 
CGCTTACTGC GTTATCTTAG AGGAAGAATT TCTGCAGAAG GACAAGTGAA TTACGGATTG 
CAGATGGCTG TAAGCTTGGC AATAGGATTC TTATTCCTTG GAGGTGGGAC ACACACATTT 
TCAACTCAGA ATAGTGCAAT TGCTGCATTA TTGATCTCAC TTTACCCACG TTTACCCACT 
GGACCAAATG ACAATCGCTG CCATCTCCAG GCATTCAGAC ACCTATATGT AATAGCTACA 
GAGCCTCGAT GGGTTCAGAC GGTGGACGTC GACACAGAAC TTCCAGTGTA TTGTCCATTG 
GAAGTGACAG TTGCTGAAAC AGAGTATTAT GATGAGACCA ACTATTGCGA GGTGACACCT 
TGTCTTCTGC CAGAACGCTC AGTGCTTAAA AGCATTCGAG TTTGTGGGCC TAGATACTGG 
CCTCAGGTGA TAAAACTTGC ACCTGAAGAC AAGCCATGGT GGAGATCTGG AGACAAGACA 
GATCCATTCA ATGGGGGGGT ACTCTACATA AAACGAAAAG TTGGATCATG CTCCTACTCT 
GACGATCCAA TTGGATGCCA ATCCTTGATT TCACGGGCAA TGCATGAGGT CTGTGATGCA 
CCATCTGCTA GTTGCAGTAA TCAACCAAAC AGCACTAATG ATAGCTACTT CAGAGTTGAT 
CAGCTAGTAA GCACTTTCTC GGCCAATCCA AGCTTGATTG CTTTTGCAAA ACTGTGTTCC 
GTGTCATGGA AGAGCAGATG CAATAGCAAT TTTCAGGAGT TCTGCTCTCA AGTGCTGTAT 
GAATGTATGA GCAAAGATAG ACCATCACTG TTACAGGTCT ACATCAGTTT TTACACAACA 
ATAGAGTTAA TGTGGGAGCA TTTGAAGACT GGGCATTTTC CTTTATATGA TTCCCTTTTC 
CTTCCTAACT TGAAGGTCGC TTTGGCCTAC AACGAAGCAT TAGTTGATGC TAGAATCACA 
AATGGAGGAA TTATTCATTC GACATTCTTG GAGTCACTTA TGAAGCGAGT GGGAGACATC 
TTTGCGGAGC TGCCAAACCT CGAGGACAAC CTCCACCGTT ACTTGAGCAT GGGCACATGG 
CCTGATACCC AAAACGACAT CGTGGTCCTC TCGTGGTATC TCCATTGGTA CAATATACCA 
CCTCCCCATG TTGTGGCTTC TGCAGTTGAG AAGGTCAGGC CCAGAGTTCC TGCCGGTGTA 
TCTATGCTCC CTCTGCTTCG CCTCCTGCTG CCAAACACGC ACCTTGTGGG ACTAATGGAG 
ATAGAGAAGC TCCACATTGT AATGGAATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021303616.1
CDS	187..5619
Translation	MASAIGSRRLTVLREFRPHGLAVEEADGEGAPGAPPPQDYDYFLFDPALAVSPSPDTSNEASSSGADGDHELFIRGNQIIWSNGSRVHKRYVSPNTVKMACWCRMNAISDALLCVLQVDTLSLYNVTGEIVSIPLPYAVSSIWPLPFGLLLQKSTDGGHMVSSSSSLLNARDLNRPSKEYGLTYNVPFQANTLETDSKDNGAIVSSHLILKHPLEEPQATYVEEKDKLTMMRDFDEETIWTSDMIPLIASYHKGKFQHSVWQIDGASYQEAMNENTMLPVDISSHKCAFRKIWQGECSESAASKVFLATDIDGMPIISFLLDEQKILLAVRIQVDDTSEEALGDIKPHMSWNIPAFAAVPVVVTRPRVRVGVLPFTDILVLSSDNDLLLYSGKQCLCKYTLPNELGKGFFSNHELNSDISDIYSDLKITSIADAVEGRINVTCSNGLMLRCSLRKNPSSSLVSDCITAMAEGLQSCFYNQFVSLFWGDSDAAYLHSSSQVDSEWEYFSHEIKRVCTKYGQTLSTKSLTLPSKAWDFLINSKYHSQYCKRALSSSNSLLPASYNTCKTVFNPFLQDEHSLDVPYNIRFMRETLETLHALYENLKLNILRKEDLGRLASLLCTVASSLGEHSYVDYYCRDFPLNLIESPSLVSSNSLTTPPSLFRWFEYCLCHGLDSAKLEDIPTLMRKQKVSAVSWGRKVLSFYSLLLGAERKGKNLSSGVYCEVASGSARNTEELTVLAMVAEKFGHQQLDLLPVGVSLVLRHALDKCRDSPPDDWPATAYVLVGREDLAAAKMGTVRKENLLWNNDNLTSMSVPYMLHLQPVIIPTTAPDIPASEVLNSDDSDSVYKSVEDGMEHIFTSTTQLRFGHDLRLNEVRRLLCSARPVAIQTPTNPSVSDQDLHQQQLWNFAQRTTALPFGRGAFTLATTYTLLTEVLVFPKLVLAGRLPAQQNATVNLDLSSRSVSEFKSWAEFHNGVAAGLRLAPFQEKMLRTWIQYNRPSEPNFTHAGLLLAFGLHEHLRVLTMTDAYRYLSQEHDITTLGLLLGLAASHRGTMDPAISKMLYFHVPSRHPSSTPELELPTLLQSAAVMGIGLLYEGSAHALTMKILLGEIGRRSGGDNVLEREGYAVAAGSALGFVALGHGNCAFEFMDTFLDRLFEYIGSKEVYHEKYLNATTADDQSCNPGQMMDGTQINVDVTAPGAIIALTLIFLKAESEEIAARLNIPNTYFDLQYVRPDFVMLRIIARNLILWSRIQPTKGWVDSQIPETVKSGVSNMSEGTIDSDELDAEALFQAYVNIVTGACIALGLKYAGSRNGDAQELLYAYTVHFLNEIKHIPVETSNILPKGLLQYVDRGTLELCLHLIVLSLSLVMAGSGNLQTFRLLRYLRGRISAEGQVNYGLQMAVSLAIGFLFLGGGTHTFSTQNSAIAALLISLYPRLPTGPNDNRCHLQAFRHLYVIATEPRWVQTVDVDTELPVYCPLEVTVAETEYYDETNYCEVTPCLLPERSVLKSIRVCGPRYWPQVIKLAPEDKPWWRSGDKTDPFNGGVLYIKRKVGSCSYSDDPIGCQSLISRAMHEVCDAPSASCSNQPNSTNDSYFRVDQLVSTFSANPSLIAFAKLCSVSWKSRCNSNFQEFCSQVLYECMSKDRPSLLQVYISFYTTIELMWEHLKTGHFPLYDSLFLPNLKVALAYNEALVDARITNGGIIHSTFLESLMKRVGDIFAELPNLEDNLHRYLSMGTWPDTQNDIVVLSWYLHWYNIPPPHVVASAVEKVRPRVPAGVSMLPLLRLLLPNTHLVGLMEIEKLHIVMEY

Target ORF information:

RefSeq Version	XM_021447941.1
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor anaphase-promoting complex subunit 1 (LOC8061339), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021447941.1

ORF Insert Sequence:

ATGGCTTCGG CGATCGGTTC GCGGCGGCTA ACGGTGCTGC GAGAGTTCCG TCCTCACGGG 
CTCGCAGTCG AGGAGGCCGA CGGCGAGGGC GCCCCCGGGG CGCCGCCGCC GCAGGATTAC 
GACTACTTCC TCTTTGACCC CGCCCTCGCC GTTTCCCCCA GCCCCGACAC CAGCAATGAG 
GCCTCCTCCT CCGGCGCAGA CGGCGACCAC GAGCTCTTCA TCCGTGGCAA TCAGATAATA 
TGGAGCAATG GTTCTCGAGT TCACAAAAGA TATGTATCAC CTAATACAGT CAAGATGGCT 
TGCTGGTGTA GGATGAATGC AATCAGCGAT GCTCTTCTAT GTGTCCTGCA AGTTGATACT 
TTATCTCTAT ACAATGTAAC TGGTGAAATT GTTAGTATTC CCCTACCTTA TGCTGTTTCA 
TCAATATGGC CACTCCCCTT TGGTCTTCTA CTTCAGAAAT CAACTGATGG AGGTCATATG 
GTGTCATCCT CAAGTTCACT GCTAAATGCA AGGGATTTAA ATCGTCCTAG CAAGGAATAT 
GGATTGACCT ACAATGTTCC GTTCCAAGCA AACACATTGG AGACAGACAG CAAAGATAAT 
GGAGCCATTG TCTCATCACA CCTCATCTTG AAACATCCAC TTGAAGAGCC TCAGGCAACA 
TATGTTGAAG AAAAGGACAA ACTAACTATG ATGAGAGATT TTGATGAAGA AACTATCTGG 
ACAAGTGACA TGATACCTCT AATAGCATCA TATCATAAAG GGAAGTTTCA GCATTCTGTC 
TGGCAAATTG ATGGTGCTAG TTACCAGGAA GCAATGAATG AAAATACAAT GCTACCTGTC 
GATATTTCTT CACATAAATG TGCATTCCGC AAGATATGGC AAGGGGAATG CTCCGAGTCT 
GCTGCTAGTA AGGTTTTCTT GGCAACTGAT ATTGATGGGA TGCCAATAAT TAGTTTTTTA 
CTGGATGAGC AAAAAATATT GCTTGCTGTA AGGATTCAAG TTGATGATAC TAGTGAAGAA 
GCTTTGGGTG ATATTAAGCC TCACATGAGC TGGAACATTC CTGCATTTGC TGCTGTGCCA 
GTTGTCGTGA CTCGTCCAAG AGTACGGGTT GGTGTTCTTC CCTTTACAGA CATCCTTGTT 
CTGAGTTCAG ATAATGATCT ACTTCTCTAT TCTGGGAAGC AGTGCCTCTG CAAATATACT 
CTCCCTAATG AATTGGGAAA GGGTTTCTTT TCAAATCATG AGCTAAATTC AGATATTTCA 
GATATTTACT CTGATCTGAA GATCACAAGC ATAGCTGATG CTGTTGAAGG CCGTATAAAC 
GTGACATGCA GTAATGGCCT GATGCTCAGA TGTTCGTTGC GCAAAAATCC TTCCTCTTCT 
TTGGTTAGTG ACTGCATAAC TGCAATGGCA GAGGGTTTGC AGAGTTGCTT TTACAATCAG 
TTTGTTTCTC TTTTTTGGGG TGATAGTGAT GCTGCCTACC TACATTCAAG CTCTCAGGTG 
GATTCAGAAT GGGAATATTT TAGTCATGAA ATAAAAAGAG TTTGTACAAA GTATGGACAA 
ACTTTGTCAA CTAAATCTCT CACTTTGCCA AGTAAAGCTT GGGACTTCCT GATCAACAGC 
AAATACCATT CCCAATACTG CAAAAGGGCC CTATCCTCTT CCAATTCACT TTTGCCTGCG 
TCATATAATA CATGCAAAAC TGTCTTTAAT CCCTTTCTCC AGGATGAGCA TAGTTTAGAT 
GTGCCATATA ATATTCGGTT CATGAGAGAA ACATTGGAGA CATTGCATGC CCTATATGAG 
AACTTGAAGC TTAACATCTT GCGGAAAGAG GATCTAGGAC GTCTCGCTTC ATTGTTATGT 
ACTGTTGCTT CATCTCTGGG TGAACATAGT TATGTTGATT ATTATTGCCG TGACTTTCCG 
CTCAACTTGA TTGAATCCCC ATCCCTAGTC TCTTCAAATT CTTTAACAAC TCCGCCGTCC 
CTGTTTCGTT GGTTTGAATA CTGCCTATGT CATGGTTTGG ACTCGGCAAA GCTGGAAGAC 
ATTCCTACTC TAATGCGCAA ACAGAAAGTT TCTGCTGTGA GTTGGGGTAG GAAGGTGTTG 
TCATTTTATA GTTTGTTGTT AGGAGCAGAA AGAAAAGGCA AAAATCTCTC TTCTGGTGTG 
TATTGTGAAG TTGCCAGTGG TTCAGCCAGA AACACTGAGG AGCTTACTGT TCTGGCTATG 
GTTGCTGAAA AGTTTGGTCA TCAGCAATTG GATTTATTAC CAGTTGGTGT ATCTCTTGTG 
CTTCGCCATG CACTGGACAA ATGTCGTGAC TCTCCACCTG ATGATTGGCC TGCAACTGCT 
TATGTTCTTG TTGGCAGGGA AGACCTAGCT GCAGCAAAGA TGGGGACAGT TAGGAAAGAA 
AACCTGTTGT GGAACAATGA TAATTTGACA TCAATGTCAG TCCCTTATAT GCTACATCTG 
CAACCTGTAA TCATACCAAC AACTGCACCT GACATACCTG CATCAGAAGT TTTGAATTCT 
GATGATTCAG ATTCTGTCTA TAAATCTGTT GAGGATGGCA TGGAACATAT CTTCACTTCA 
ACAACACAAC TCCGATTTGG TCATGATTTG CGCTTGAATG AGGTTAGACG CCTCTTATGC 
TCAGCAAGGC CAGTGGCTAT ACAAACACCT ACTAATCCTA GCGTGTCGGA TCAAGACCTA 
CATCAGCAAC AATTATGGAA TTTTGCACAA AGGACAACTG CTTTACCTTT TGGGCGTGGG 
GCTTTTACTT TAGCTACAAC TTACACTTTG TTGACAGAGG TTCTGGTGTT CCCAAAGCTT 
GTGCTGGCTG GTCGTTTGCC TGCACAGCAG AATGCCACAG TAAATCTTGA CCTAAGTAGT 
AGAAGTGTCT CAGAATTCAA ATCTTGGGCT GAGTTTCATA ATGGTGTTGC TGCTGGCCTG 
AGGCTTGCCC CTTTTCAGGA GAAAATGTTG AGAACTTGGA TACAATACAA TAGACCTTCT 
GAACCAAATT TTACTCATGC TGGTTTGCTT CTAGCATTTG GCTTGCATGA GCATCTAAGA 
GTCTTAACTA TGACTGATGC ATATCGATAC CTCTCACAGG AGCATGATAT AACAACACTT 
GGTTTGTTAC TTGGTTTGGC AGCATCTCAT AGGGGAACAA TGGATCCTGC AATATCAAAG 
ATGCTCTACT TTCATGTTCC TTCTCGACAT CCATCTTCTA CTCCAGAGCT GGAGTTACCA 
ACTCTTCTAC AGTCAGCAGC AGTAATGGGA ATTGGACTTC TATATGAAGG CTCAGCTCAT 
GCTCTCACCA TGAAGATACT TCTGGGTGAG ATTGGCCGAA GAAGTGGAGG TGACAACGTG 
CTAGAAAGGG AAGGGTATGC CGTTGCTGCC GGTTCTGCAT TAGGATTTGT TGCCTTGGGT 
CATGGCAATT GTGCATTTGA ATTCATGGAT ACTTTCCTAG ATCGACTTTT TGAATATATT 
GGCAGTAAAG AAGTCTACCA TGAAAAGTAC CTAAATGCAA CAACTGCCGA TGATCAAAGT 
TGCAACCCTG GACAGATGAT GGATGGAACA CAAATAAATG TTGATGTTAC TGCACCTGGA 
GCAATAATTG CTCTAACCTT GATATTTTTG AAAGCCGAGT CTGAGGAGAT TGCAGCTAGA 
CTCAATATCC CCAATACTTA CTTTGATTTG CAGTATGTGA GGCCTGATTT TGTTATGCTT 
CGCATTATAG CCAGGAATTT AATATTGTGG AGCAGAATAC AACCTACCAA AGGATGGGTT 
GATTCTCAAA TTCCTGAAAC AGTCAAGTCT GGTGTTTCCA ACATGAGTGA AGGGACCATT 
GACAGTGATG AATTGGATGC TGAAGCTCTT TTTCAAGCTT ATGTTAACAT AGTGACTGGA 
GCATGTATTG CACTTGGACT CAAGTATGCT GGCAGCAGAA ATGGTGATGC CCAAGAGCTA 
CTCTACGCTT ACACTGTCCA TTTTCTAAAT GAGATTAAGC ACATACCTGT TGAAACATCA 
AATATACTAC CAAAGGGATT ACTACAATAC GTTGACCGTG GAACTCTTGA ACTCTGCTTG 
CATCTTATTG TTCTATCTCT CTCTCTGGTA ATGGCAGGAT CTGGAAATTT GCAGACTTTC 
CGCTTACTGC GTTATCTTAG AGGAAGAATT TCTGCAGAAG GACAAGTGAA TTACGGATTG 
CAGATGGCTG TAAGCTTGGC AATAGGATTC TTATTCCTTG GAGGTGGGAC ACACACATTT 
TCAACTCAGA ATAGTGCAAT TGCTGCATTA TTGATCTCAC TTTACCCACG TTTACCCACT 
GGACCAAATG ACAATCGCTG CCATCTCCAG GCATTCAGAC ACCTATATGT AATAGCTACA 
GAGCCTCGAT GGGTTCAGAC GGTGGACGTC GACACAGAAC TTCCAGTGTA TTGTCCATTG 
GAAGTGACAG TTGCTGAAAC AGAGTATTAT GATGAGACCA ACTATTGCGA GGTGACACCT 
TGTCTTCTGC CAGAACGCTC AGTGCTTAAA AGCATTCGAG TTTGTGGGCC TAGATACTGG 
CCTCAGGTGA TAAAACTTGC ACCTGAAGAC AAGCCATGGT GGAGATCTGG AGACAAGACA 
GATCCATTCA ATGGGGGGGT ACTCTACATA AAACGAAAAG TTGGATCATG CTCCTACTCT 
GACGATCCAA TTGGATGCCA ATCCTTGATT TCACGGGCAA TGCATGAGGT CTGTGATGCA 
CCATCTGCTA GTTGCAGTAA TCAACCAAAC AGCACTAATG ATAGCTACTT CAGAGTTGAT 
CAGCTAGTAA GCACTTTCTC GGCCAATCCA AGCTTGATTG CTTTTGCAAA ACTGTGTTCC 
GTGTCATGGA AGAGCAGATG CAATAGCAAT TTTCAGGAGT TCTGCTCTCA AGTGCTGTAT 
GAATGTATGA GCAAAGATAG ACCATCACTG TTACAGGTCT ACATCAGTTT TTACACAACA 
ATAGAGTTAA TGTGGGAGCA TTTGAAGACT GGGCATTTTC CTTTATATGA TTCCCTTTTC 
CTTCCTAACT TGAAGGTCGC TTTGGCCTAC AACGAAGCAT TAGTTGATGC TAGAATCACA 
AATGGAGGAA TTATTCATTC GACATTCTTG GAGTCACTTA TGAAGCGAGT GGGAGACATC 
TTTGCGGAGC TGCCAAACCT CGAGGACAAC CTCCACCGTT ACTTGAGCAT GGGCACATGG 
CCTGATACCC AAAACGACAT CGTGGTCCTC TCGTGGTATC TCCATTGGTA CAATATACCA 
CCTCCCCATG TTGTGGCTTC TGCAGTTGAG AAGGTCAGGC CCAGAGTTCC TGCCGGTGTA 
TCTATGCTCC CTCTGCTTCG CCTCCTGCTG CCAAACACGC ACCTTGTGGG ACTAATGGAG 
ATAGAGAAGC TCCACATTGT AATGGAATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8061339 ( XM_021447942.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_021303617.1 Sorghum bicolor anaphase-promoting complex subunit 1 (LOC8061339), transcript variant X2, mRNA.

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CloneID	OSc105247
Clone ID Related Accession (Same CDS sequence)	XM_021447942.1
Accession Version	XM_021447942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	anaphase-promoting complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012878.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CGATCGGTTC GCGGCGGCTA ACGGTGCTGC GAGAGTTCCG TCCTCACGGG 
CTCGCAGTCG AGGAGGCCGA CGGCGAGGGC GCCCCCGGGG CGCCGCCGCC GCAGGATTAC 
GACTACTTCC TCTTTGACCC CGCCCTCGCC GTTTCCCCCA GCCCCGACAC CAGCAATGAG 
GCCTCCTCCT CCGGCGCAGA CGGCGACCAC GAGCTCTTCA TCCGTGGCAA TCAGATAATA 
TGGAGCAATG GTTCTCGAGT TCACAAAAGA TATGTATCAC CTAATACAGT CAAGATGGCT 
TGCTGGTGTA GGATGAATGC AATCAGCGAT GCTCTTCTAT GTGTCCTGCA AGTTGATACT 
TTATCTCTAT ACAATGTAAC TGGTGAAATT GTTAGTATTC CCCTACCTTA TGCTGTTTCA 
TCAATATGGC CACTCCCCTT TGGTCTTCTA CTTCAGAAAT CAACTGATGG AGGTCATATG 
GTGTCATCCT CAAGTTCACT GCTAAATGCA AGGGATTTAA ATCGTCCTAG CAAGGAATAT 
GGATTGACCT ACAATGTTCC GTTCCAAGCA AACACATTGG AGACAGACAG CAAAGATAAT 
GGAGCCATTG TCTCATCACA CCTCATCTTG AAACATCCAC TTGAAGAGCC TCAGGCAACA 
TATGTTGAAG AAAAGGACAA ACTAACTATG ATGAGAGATT TTGATGAAGA AACTATCTGG 
ACAAGTGACA TGATACCTCT AATAGCATCA TATCATAAAG GGAAGTTTCA GCATTCTGTC 
TGGCAAATTG ATGGTGCTAG TTACCAGGAA GCAATGAATG AAAATACAAT GCTACCTGTC 
GATATTTCTT CACATAAATG TGCATTCCGC AAGATATGGC AAGGGGAATG CTCCGAGTCT 
GCTGCTAGTA AGGTTTTCTT GGCAACTGAT ATTGATGGGA TGCCAATAAT TAGTTTTTTA 
CTGGATGAGC AAAAAATATT GCTTGCTGTA AGGATTCAAG TTGATGATAC TAGTGAAGAA 
GCTTTGGGTG ATATTAAGCC TCACATGAGC TGGAACATTC CTGCATTTGC TGCTGTGCCA 
GTTGTCGTGA CTCGTCCAAG AGTACGGGTT GGTGTTCTTC CCTTTACAGA CATCCTTGTT 
CTGAGTTCAG ATAATGATCT ACTTCTCTAT TCTGGGAAGC AGTGCCTCTG CAAATATACT 
CTCCCTAATG AATTGGGAAA GGGTTTCTTT TCAAATCATG AGCTAAATTC AGATATTTCA 
GATATTTACT CTGATCTGAA GATCACAAGC ATAGCTGATG CTGTTGAAGG CCGTATAAAC 
GTGACATGCA GTAATGGCCT GATGCTCAGA TGTTCGTTGC GCAAAAATCC TTCCTCTTCT 
TTGGTTAGTG ACTGCATAAC TGCAATGGCA GAGGGTTTGC AGAGTTGCTT TTACAATCAG 
TTTGTTTCTC TTTTTTGGGG TGATAGTGAT GCTGCCTACC TACATTCAAG CTCTCAGGTG 
GATTCAGAAT GGGAATATTT TAGTCATGAA ATAAAAAGAG TTTGTACAAA GTATGGACAA 
ACTTTGTCAA CTAAATCTCT CACTTTGCCA AGTAAAGCTT GGGACTTCCT GATCAACAGC 
AAATACCATT CCCAATACTG CAAAAGGGCC CTATCCTCTT CCAATTCACT TTTGCCTGCG 
TCATATAATA CATGCAAAAC TGTCTTTAAT CCCTTTCTCC AGGATGAGCA TAGTTTAGAT 
GTGCCATATA ATATTCGGTT CATGAGAGAA ACATTGGAGA CATTGCATGC CCTATATGAG 
AACTTGAAGC TTAACATCTT GCGGAAAGAG GATCTAGGAC GTCTCGCTTC ATTGTTATGT 
ACTGTTGCTT CATCTCTGGG TGAACATAGT TATGTTGATT ATTATTGCCG TGACTTTCCG 
CTCAACTTGA TTGAATCCCC ATCCCTAGTC TCTTCAAATT CTTTAACAAC TCCGCCGTCC 
CTGTTTCGTT GGTTTGAATA CTGCCTATGT CATGGTTTGG ACTCGGCAAA GCTGGAAGAC 
ATTCCTACTC TAATGCGCAA ACAGAAAGTT TCTGCTGTGA GTTGGGGTAG GAAGGTGTTG 
TCATTTTATA GTTTGTTGTT AGGAGCAGAA AGAAAAGGCA AAAATCTCTC TTCTGGTGTG 
TATTGTGAAG TTGCCAGTGG TTCAGCCAGA AACACTGAGG AGCTTACTGT TCTGGCTATG 
GTTGCTGAAA AGTTTGGTCA TCAGCAATTG GATTTATTAC CAGTTGGTGT ATCTCTTGTG 
CTTCGCCATG CACTGGACAA ATGTCGTGAC TCTCCACCTG ATGATTGGCC TGCAACTGCT 
TATGTTCTTG TTGGCAGGGA AGACCTAGCT GCAGCAAAGA TGGGGACAGT TAGGAAAGAA 
AACCTGTTGT GGAACAATGA TAATTTGACA TCAATGTCAG TCCCTTATAT GCTACATCTG 
CAACCTGTAA TCATACCAAC AACTGCACCT GACATACCTG CATCAGAAGT TTTGAATTCT 
GATGATTCAG ATTCTGTCTA TAAATCTGTT GAGGATGGCA TGGAACATAT CTTCACTTCA 
ACAACACAAC TCCGATTTGG TCATGATTTG CGCTTGAATG AGGTTAGACG CCTCTTATGC 
TCAGCAAGGC CAGTGGCTAT ACAAACACCT ACTAATCCTA GCGTGTCGGA TCAAGACCTA 
CATCAGCAAC AATTATGGAA TTTTGCACAA AGGACAACTG CTTTACCTTT TGGGCGTGGG 
GCTTTTACTT TAGCTACAAC TTACACTTTG TTGACAGAGG TTCTGGTGTT CCCAAAGCTT 
GTGCTGGCTG GTCGTTTGCC TGCACAGCAG AATGCCACAG TAAATCTTGA CCTAAGTAGT 
AGAAGTGTCT CAGAATTCAA ATCTTGGGCT GAGTTTCATA ATGGTGTTGC TGCTGGCCTG 
AGGCTTGCCC CTTTTCAGGA GAAAATGTTG AGAACTTGGA TACAATACAA TAGACCTTCT 
GAACCAAATT TTACTCATGC TGGTTTGCTT CTAGCATTTG GCTTGCATGA GCATCTAAGA 
GTCTTAACTA TGACTGATGC ATATCGATAC CTCTCACAGG AGCATGATAT AACAACACTT 
GGTTTGTTAC TTGGTTTGGC AGCATCTCAT AGGGGAACAA TGGATCCTGC AATATCAAAG 
ATGCTCTACT TTCATGTTCC TTCTCGACAT CCATCTTCTA CTCCAGAGCT GGAGTTACCA 
ACTCTTCTAC AGTCAGCAGC AGTAATGGGA ATTGGACTTC TATATGAAGG CTCAGCTCAT 
GCTCTCACCA TGAAGATACT TCTGGAAAAG TACCTAAATG CAACAACTGC CGATGATCAA 
AGTTGCAACC CTGGACAGAT GATGGATGGA ACACAAATAA ATGTTGATGT TACTGCACCT 
GGAGCAATAA TTGCTCTAAC CTTGATATTT TTGAAAGCCG AGTCTGAGGA GATTGCAGCT 
AGACTCAATA TCCCCAATAC TTACTTTGAT TTGCAGTATG TGAGGCCTGA TTTTGTTATG 
CTTCGCATTA TAGCCAGGAA TTTAATATTG TGGAGCAGAA TACAACCTAC CAAAGGATGG 
GTTGATTCTC AAATTCCTGA AACAGTCAAG TCTGGTGTTT CCAACATGAG TGAAGGGACC 
ATTGACAGTG ATGAATTGGA TGCTGAAGCT CTTTTTCAAG CTTATGTTAA CATAGTGACT 
GGAGCATGTA TTGCACTTGG ACTCAAGTAT GCTGGCAGCA GAAATGGTGA TGCCCAAGAG 
CTACTCTACG CTTACACTGT CCATTTTCTA AATGAGATTA AGCACATACC TGTTGAAACA 
TCAAATATAC TACCAAAGGG ATTACTACAA TACGTTGACC GTGGAACTCT TGAACTCTGC 
TTGCATCTTA TTGTTCTATC TCTCTCTCTG GTAATGGCAG GATCTGGAAA TTTGCAGACT 
TTCCGCTTAC TGCGTTATCT TAGAGGAAGA ATTTCTGCAG AAGGACAAGT GAATTACGGA 
TTGCAGATGG CTGTAAGCTT GGCAATAGGA TTCTTATTCC TTGGAGGTGG GACACACACA 
TTTTCAACTC AGAATAGTGC AATTGCTGCA TTATTGATCT CACTTTACCC ACGTTTACCC 
ACTGGACCAA ATGACAATCG CTGCCATCTC CAGGCATTCA GACACCTATA TGTAATAGCT 
ACAGAGCCTC GATGGGTTCA GACGGTGGAC GTCGACACAG AACTTCCAGT GTATTGTCCA 
TTGGAAGTGA CAGTTGCTGA AACAGAGTAT TATGATGAGA CCAACTATTG CGAGGTGACA 
CCTTGTCTTC TGCCAGAACG CTCAGTGCTT AAAAGCATTC GAGTTTGTGG GCCTAGATAC 
TGGCCTCAGG TGATAAAACT TGCACCTGAA GACAAGCCAT GGTGGAGATC TGGAGACAAG 
ACAGATCCAT TCAATGGGGG GGTACTCTAC ATAAAACGAA AAGTTGGATC ATGCTCCTAC 
TCTGACGATC CAATTGGATG CCAATCCTTG ATTTCACGGG CAATGCATGA GGTCTGTGAT 
GCACCATCTG CTAGTTGCAG TAATCAACCA AACAGCACTA ATGATAGCTA CTTCAGAGTT 
GATCAGCTAG TAAGCACTTT CTCGGCCAAT CCAAGCTTGA TTGCTTTTGC AAAACTGTGT 
TCCGTGTCAT GGAAGAGCAG ATGCAATAGC AATTTTCAGG AGTTCTGCTC TCAAGTGCTG 
TATGAATGTA TGAGCAAAGA TAGACCATCA CTGTTACAGG TCTACATCAG TTTTTACACA 
ACAATAGAGT TAATGTGGGA GCATTTGAAG ACTGGGCATT TTCCTTTATA TGATTCCCTT 
TTCCTTCCTA ACTTGAAGGT CGCTTTGGCC TACAACGAAG CATTAGTTGA TGCTAGAATC 
ACAAATGGAG GAATTATTCA TTCGACATTC TTGGAGTCAC TTATGAAGCG AGTGGGAGAC 
ATCTTTGCGG AGCTGCCAAA CCTCGAGGAC AACCTCCACC GTTACTTGAG CATGGGCACA 
TGGCCTGATA CCCAAAACGA CATCGTGGTC CTCTCGTGGT ATCTCCATTG GTACAATATA 
CCACCTCCCC ATGTTGTGGC TTCTGCAGTT GAGAAGGTCA GGCCCAGAGT TCCTGCCGGT 
GTATCTATGC TCCCTCTGCT TCGCCTCCTG CTGCCAAACA CGCACCTTGT GGGACTAATG 
GAGATAGAGA AGCTCCACAT TGTAATGGAA TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021303617.1
CDS	187..5442
Translation	MASAIGSRRLTVLREFRPHGLAVEEADGEGAPGAPPPQDYDYFLFDPALAVSPSPDTSNEASSSGADGDHELFIRGNQIIWSNGSRVHKRYVSPNTVKMACWCRMNAISDALLCVLQVDTLSLYNVTGEIVSIPLPYAVSSIWPLPFGLLLQKSTDGGHMVSSSSSLLNARDLNRPSKEYGLTYNVPFQANTLETDSKDNGAIVSSHLILKHPLEEPQATYVEEKDKLTMMRDFDEETIWTSDMIPLIASYHKGKFQHSVWQIDGASYQEAMNENTMLPVDISSHKCAFRKIWQGECSESAASKVFLATDIDGMPIISFLLDEQKILLAVRIQVDDTSEEALGDIKPHMSWNIPAFAAVPVVVTRPRVRVGVLPFTDILVLSSDNDLLLYSGKQCLCKYTLPNELGKGFFSNHELNSDISDIYSDLKITSIADAVEGRINVTCSNGLMLRCSLRKNPSSSLVSDCITAMAEGLQSCFYNQFVSLFWGDSDAAYLHSSSQVDSEWEYFSHEIKRVCTKYGQTLSTKSLTLPSKAWDFLINSKYHSQYCKRALSSSNSLLPASYNTCKTVFNPFLQDEHSLDVPYNIRFMRETLETLHALYENLKLNILRKEDLGRLASLLCTVASSLGEHSYVDYYCRDFPLNLIESPSLVSSNSLTTPPSLFRWFEYCLCHGLDSAKLEDIPTLMRKQKVSAVSWGRKVLSFYSLLLGAERKGKNLSSGVYCEVASGSARNTEELTVLAMVAEKFGHQQLDLLPVGVSLVLRHALDKCRDSPPDDWPATAYVLVGREDLAAAKMGTVRKENLLWNNDNLTSMSVPYMLHLQPVIIPTTAPDIPASEVLNSDDSDSVYKSVEDGMEHIFTSTTQLRFGHDLRLNEVRRLLCSARPVAIQTPTNPSVSDQDLHQQQLWNFAQRTTALPFGRGAFTLATTYTLLTEVLVFPKLVLAGRLPAQQNATVNLDLSSRSVSEFKSWAEFHNGVAAGLRLAPFQEKMLRTWIQYNRPSEPNFTHAGLLLAFGLHEHLRVLTMTDAYRYLSQEHDITTLGLLLGLAASHRGTMDPAISKMLYFHVPSRHPSSTPELELPTLLQSAAVMGIGLLYEGSAHALTMKILLEKYLNATTADDQSCNPGQMMDGTQINVDVTAPGAIIALTLIFLKAESEEIAARLNIPNTYFDLQYVRPDFVMLRIIARNLILWSRIQPTKGWVDSQIPETVKSGVSNMSEGTIDSDELDAEALFQAYVNIVTGACIALGLKYAGSRNGDAQELLYAYTVHFLNEIKHIPVETSNILPKGLLQYVDRGTLELCLHLIVLSLSLVMAGSGNLQTFRLLRYLRGRISAEGQVNYGLQMAVSLAIGFLFLGGGTHTFSTQNSAIAALLISLYPRLPTGPNDNRCHLQAFRHLYVIATEPRWVQTVDVDTELPVYCPLEVTVAETEYYDETNYCEVTPCLLPERSVLKSIRVCGPRYWPQVIKLAPEDKPWWRSGDKTDPFNGGVLYIKRKVGSCSYSDDPIGCQSLISRAMHEVCDAPSASCSNQPNSTNDSYFRVDQLVSTFSANPSLIAFAKLCSVSWKSRCNSNFQEFCSQVLYECMSKDRPSLLQVYISFYTTIELMWEHLKTGHFPLYDSLFLPNLKVALAYNEALVDARITNGGIIHSTFLESLMKRVGDIFAELPNLEDNLHRYLSMGTWPDTQNDIVVLSWYLHWYNIPPPHVVASAVEKVRPRVPAGVSMLPLLRLLLPNTHLVGLMEIEKLHIVMEY

Target ORF information:

RefSeq Version	XM_021447942.1
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor anaphase-promoting complex subunit 1 (LOC8061339), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021447942.1

ORF Insert Sequence:

ATGGCTTCGG CGATCGGTTC GCGGCGGCTA ACGGTGCTGC GAGAGTTCCG TCCTCACGGG 
CTCGCAGTCG AGGAGGCCGA CGGCGAGGGC GCCCCCGGGG CGCCGCCGCC GCAGGATTAC 
GACTACTTCC TCTTTGACCC CGCCCTCGCC GTTTCCCCCA GCCCCGACAC CAGCAATGAG 
GCCTCCTCCT CCGGCGCAGA CGGCGACCAC GAGCTCTTCA TCCGTGGCAA TCAGATAATA 
TGGAGCAATG GTTCTCGAGT TCACAAAAGA TATGTATCAC CTAATACAGT CAAGATGGCT 
TGCTGGTGTA GGATGAATGC AATCAGCGAT GCTCTTCTAT GTGTCCTGCA AGTTGATACT 
TTATCTCTAT ACAATGTAAC TGGTGAAATT GTTAGTATTC CCCTACCTTA TGCTGTTTCA 
TCAATATGGC CACTCCCCTT TGGTCTTCTA CTTCAGAAAT CAACTGATGG AGGTCATATG 
GTGTCATCCT CAAGTTCACT GCTAAATGCA AGGGATTTAA ATCGTCCTAG CAAGGAATAT 
GGATTGACCT ACAATGTTCC GTTCCAAGCA AACACATTGG AGACAGACAG CAAAGATAAT 
GGAGCCATTG TCTCATCACA CCTCATCTTG AAACATCCAC TTGAAGAGCC TCAGGCAACA 
TATGTTGAAG AAAAGGACAA ACTAACTATG ATGAGAGATT TTGATGAAGA AACTATCTGG 
ACAAGTGACA TGATACCTCT AATAGCATCA TATCATAAAG GGAAGTTTCA GCATTCTGTC 
TGGCAAATTG ATGGTGCTAG TTACCAGGAA GCAATGAATG AAAATACAAT GCTACCTGTC 
GATATTTCTT CACATAAATG TGCATTCCGC AAGATATGGC AAGGGGAATG CTCCGAGTCT 
GCTGCTAGTA AGGTTTTCTT GGCAACTGAT ATTGATGGGA TGCCAATAAT TAGTTTTTTA 
CTGGATGAGC AAAAAATATT GCTTGCTGTA AGGATTCAAG TTGATGATAC TAGTGAAGAA 
GCTTTGGGTG ATATTAAGCC TCACATGAGC TGGAACATTC CTGCATTTGC TGCTGTGCCA 
GTTGTCGTGA CTCGTCCAAG AGTACGGGTT GGTGTTCTTC CCTTTACAGA CATCCTTGTT 
CTGAGTTCAG ATAATGATCT ACTTCTCTAT TCTGGGAAGC AGTGCCTCTG CAAATATACT 
CTCCCTAATG AATTGGGAAA GGGTTTCTTT TCAAATCATG AGCTAAATTC AGATATTTCA 
GATATTTACT CTGATCTGAA GATCACAAGC ATAGCTGATG CTGTTGAAGG CCGTATAAAC 
GTGACATGCA GTAATGGCCT GATGCTCAGA TGTTCGTTGC GCAAAAATCC TTCCTCTTCT 
TTGGTTAGTG ACTGCATAAC TGCAATGGCA GAGGGTTTGC AGAGTTGCTT TTACAATCAG 
TTTGTTTCTC TTTTTTGGGG TGATAGTGAT GCTGCCTACC TACATTCAAG CTCTCAGGTG 
GATTCAGAAT GGGAATATTT TAGTCATGAA ATAAAAAGAG TTTGTACAAA GTATGGACAA 
ACTTTGTCAA CTAAATCTCT CACTTTGCCA AGTAAAGCTT GGGACTTCCT GATCAACAGC 
AAATACCATT CCCAATACTG CAAAAGGGCC CTATCCTCTT CCAATTCACT TTTGCCTGCG 
TCATATAATA CATGCAAAAC TGTCTTTAAT CCCTTTCTCC AGGATGAGCA TAGTTTAGAT 
GTGCCATATA ATATTCGGTT CATGAGAGAA ACATTGGAGA CATTGCATGC CCTATATGAG 
AACTTGAAGC TTAACATCTT GCGGAAAGAG GATCTAGGAC GTCTCGCTTC ATTGTTATGT 
ACTGTTGCTT CATCTCTGGG TGAACATAGT TATGTTGATT ATTATTGCCG TGACTTTCCG 
CTCAACTTGA TTGAATCCCC ATCCCTAGTC TCTTCAAATT CTTTAACAAC TCCGCCGTCC 
CTGTTTCGTT GGTTTGAATA CTGCCTATGT CATGGTTTGG ACTCGGCAAA GCTGGAAGAC 
ATTCCTACTC TAATGCGCAA ACAGAAAGTT TCTGCTGTGA GTTGGGGTAG GAAGGTGTTG 
TCATTTTATA GTTTGTTGTT AGGAGCAGAA AGAAAAGGCA AAAATCTCTC TTCTGGTGTG 
TATTGTGAAG TTGCCAGTGG TTCAGCCAGA AACACTGAGG AGCTTACTGT TCTGGCTATG 
GTTGCTGAAA AGTTTGGTCA TCAGCAATTG GATTTATTAC CAGTTGGTGT ATCTCTTGTG 
CTTCGCCATG CACTGGACAA ATGTCGTGAC TCTCCACCTG ATGATTGGCC TGCAACTGCT 
TATGTTCTTG TTGGCAGGGA AGACCTAGCT GCAGCAAAGA TGGGGACAGT TAGGAAAGAA 
AACCTGTTGT GGAACAATGA TAATTTGACA TCAATGTCAG TCCCTTATAT GCTACATCTG 
CAACCTGTAA TCATACCAAC AACTGCACCT GACATACCTG CATCAGAAGT TTTGAATTCT 
GATGATTCAG ATTCTGTCTA TAAATCTGTT GAGGATGGCA TGGAACATAT CTTCACTTCA 
ACAACACAAC TCCGATTTGG TCATGATTTG CGCTTGAATG AGGTTAGACG CCTCTTATGC 
TCAGCAAGGC CAGTGGCTAT ACAAACACCT ACTAATCCTA GCGTGTCGGA TCAAGACCTA 
CATCAGCAAC AATTATGGAA TTTTGCACAA AGGACAACTG CTTTACCTTT TGGGCGTGGG 
GCTTTTACTT TAGCTACAAC TTACACTTTG TTGACAGAGG TTCTGGTGTT CCCAAAGCTT 
GTGCTGGCTG GTCGTTTGCC TGCACAGCAG AATGCCACAG TAAATCTTGA CCTAAGTAGT 
AGAAGTGTCT CAGAATTCAA ATCTTGGGCT GAGTTTCATA ATGGTGTTGC TGCTGGCCTG 
AGGCTTGCCC CTTTTCAGGA GAAAATGTTG AGAACTTGGA TACAATACAA TAGACCTTCT 
GAACCAAATT TTACTCATGC TGGTTTGCTT CTAGCATTTG GCTTGCATGA GCATCTAAGA 
GTCTTAACTA TGACTGATGC ATATCGATAC CTCTCACAGG AGCATGATAT AACAACACTT 
GGTTTGTTAC TTGGTTTGGC AGCATCTCAT AGGGGAACAA TGGATCCTGC AATATCAAAG 
ATGCTCTACT TTCATGTTCC TTCTCGACAT CCATCTTCTA CTCCAGAGCT GGAGTTACCA 
ACTCTTCTAC AGTCAGCAGC AGTAATGGGA ATTGGACTTC TATATGAAGG CTCAGCTCAT 
GCTCTCACCA TGAAGATACT TCTGGAAAAG TACCTAAATG CAACAACTGC CGATGATCAA 
AGTTGCAACC CTGGACAGAT GATGGATGGA ACACAAATAA ATGTTGATGT TACTGCACCT 
GGAGCAATAA TTGCTCTAAC CTTGATATTT TTGAAAGCCG AGTCTGAGGA GATTGCAGCT 
AGACTCAATA TCCCCAATAC TTACTTTGAT TTGCAGTATG TGAGGCCTGA TTTTGTTATG 
CTTCGCATTA TAGCCAGGAA TTTAATATTG TGGAGCAGAA TACAACCTAC CAAAGGATGG 
GTTGATTCTC AAATTCCTGA AACAGTCAAG TCTGGTGTTT CCAACATGAG TGAAGGGACC 
ATTGACAGTG ATGAATTGGA TGCTGAAGCT CTTTTTCAAG CTTATGTTAA CATAGTGACT 
GGAGCATGTA TTGCACTTGG ACTCAAGTAT GCTGGCAGCA GAAATGGTGA TGCCCAAGAG 
CTACTCTACG CTTACACTGT CCATTTTCTA AATGAGATTA AGCACATACC TGTTGAAACA 
TCAAATATAC TACCAAAGGG ATTACTACAA TACGTTGACC GTGGAACTCT TGAACTCTGC 
TTGCATCTTA TTGTTCTATC TCTCTCTCTG GTAATGGCAG GATCTGGAAA TTTGCAGACT 
TTCCGCTTAC TGCGTTATCT TAGAGGAAGA ATTTCTGCAG AAGGACAAGT GAATTACGGA 
TTGCAGATGG CTGTAAGCTT GGCAATAGGA TTCTTATTCC TTGGAGGTGG GACACACACA 
TTTTCAACTC AGAATAGTGC AATTGCTGCA TTATTGATCT CACTTTACCC ACGTTTACCC 
ACTGGACCAA ATGACAATCG CTGCCATCTC CAGGCATTCA GACACCTATA TGTAATAGCT 
ACAGAGCCTC GATGGGTTCA GACGGTGGAC GTCGACACAG AACTTCCAGT GTATTGTCCA 
TTGGAAGTGA CAGTTGCTGA AACAGAGTAT TATGATGAGA CCAACTATTG CGAGGTGACA 
CCTTGTCTTC TGCCAGAACG CTCAGTGCTT AAAAGCATTC GAGTTTGTGG GCCTAGATAC 
TGGCCTCAGG TGATAAAACT TGCACCTGAA GACAAGCCAT GGTGGAGATC TGGAGACAAG 
ACAGATCCAT TCAATGGGGG GGTACTCTAC ATAAAACGAA AAGTTGGATC ATGCTCCTAC 
TCTGACGATC CAATTGGATG CCAATCCTTG ATTTCACGGG CAATGCATGA GGTCTGTGAT 
GCACCATCTG CTAGTTGCAG TAATCAACCA AACAGCACTA ATGATAGCTA CTTCAGAGTT 
GATCAGCTAG TAAGCACTTT CTCGGCCAAT CCAAGCTTGA TTGCTTTTGC AAAACTGTGT 
TCCGTGTCAT GGAAGAGCAG ATGCAATAGC AATTTTCAGG AGTTCTGCTC TCAAGTGCTG 
TATGAATGTA TGAGCAAAGA TAGACCATCA CTGTTACAGG TCTACATCAG TTTTTACACA 
ACAATAGAGT TAATGTGGGA GCATTTGAAG ACTGGGCATT TTCCTTTATA TGATTCCCTT 
TTCCTTCCTA ACTTGAAGGT CGCTTTGGCC TACAACGAAG CATTAGTTGA TGCTAGAATC 
ACAAATGGAG GAATTATTCA TTCGACATTC TTGGAGTCAC TTATGAAGCG AGTGGGAGAC 
ATCTTTGCGG AGCTGCCAAA CCTCGAGGAC AACCTCCACC GTTACTTGAG CATGGGCACA 
TGGCCTGATA CCCAAAACGA CATCGTGGTC CTCTCGTGGT ATCTCCATTG GTACAATATA 
CCACCTCCCC ATGTTGTGGC TTCTGCAGTT GAGAAGGTCA GGCCCAGAGT TCCTGCCGGT 
GTATCTATGC TCCCTCTGCT TCGCCTCCTG CTGCCAAACA CGCACCTTGT GGGACTAATG 
GAGATAGAGA AGCTCCACAT TGTAATGGAA TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8061339 ( XM_002439575.2 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_002439620.2 Sorghum bicolor anaphase-promoting complex subunit 1 (LOC8061339), transcript variant X3, mRNA.

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CloneID	OSc105248
Clone ID Related Accession (Same CDS sequence)	XM_002439575.2
Accession Version	XM_002439575.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	anaphase-promoting complex subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012878.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 13, 2017 this sequence version replaced XM_002439575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAC AATGCTACCT GTCGATATTT CTTCACATAA ATGTGCATTC CGCAAGATAT 
GGCAAGGGGA ATGCTCCGAG TCTGCTGCTA GTTTTCTTGG CAACTGATAT TGATGGGATG 
CCAATAATTA GTTTTTTACT GGATGAGCAA AAAATATTGC TTGCTGTAAG GATTCAAGTT 
GATGATACTA GTGAAGAAGC TTTGGGTGAT ATTAAGCCTC ACATGAGCTG GAACATTCCT 
GCATTTGCTG CTGTGCCAGT TGTCGTGACT CGTCCAAGAG TACGGGTTGG TGTTCTTCCC 
TTTACAGACA TCCTTGTTCT GAGTTCAGAT AATGATCTAC TTCTCTATTC TGGGAAGCAG 
TGCCTCTGCA AATATACTCT CCCTAATGAA TTGGGAAAGG GTTTCTTTTC AAATCATGAG 
CTAAATTCAG ATATTTCAGA TATTTACTCT GATCTGAAGA TCACAAGCAT AGCTGATGCT 
GTTGAAGGCC GTATAAACGT GACATGCAGT AATGGCCTGA TGCTCAGATG TTCGTTGCGC 
AAAAATCCTT CCTCTTCTTT GGTTAGTGAC TGCATAACTG CAATGGCAGA GGGTTTGCAG 
AGTTGCTTTT ACAATCAGTT TGTTTCTCTT TTTTGGGGTG ATAGTGATGC TGCCTACCTA 
CATTCAAGCT CTCAGGTGGA TTCAGAATGG GAATATTTTA GTCATGAAAT AAAAAGAGTT 
TGTACAAAGT ATGGACAAAC TTTGTCAACT AAATCTCTCA CTTTGCCAAG TAAAGCTTGG 
GACTTCCTGA TCAACAGCAA ATACCATTCC CAATACTGCA AAAGGGCCCT ATCCTCTTCC 
AATTCACTTT TGCCTGCGTC ATATAATACA TGCAAAACTG TCTTTAATCC CTTTCTCCAG 
GATGAGCATA GTTTAGATGT GCCATATAAT ATTCGGTTCA TGAGAGAAAC ATTGGAGACA 
TTGCATGCCC TATATGAGAA CTTGAAGCTT AACATCTTGC GGAAAGAGGA TCTAGGACGT 
CTCGCTTCAT TGTTATGTAC TGTTGCTTCA TCTCTGGGTG AACATAGTTA TGTTGATTAT 
TATTGCCGTG ACTTTCCGCT CAACTTGATT GAATCCCCAT CCCTAGTCTC TTCAAATTCT 
TTAACAACTC CGCCGTCCCT GTTTCGTTGG TTTGAATACT GCCTATGTCA TGGTTTGGAC 
TCGGCAAAGC TGGAAGACAT TCCTACTCTA ATGCGCAAAC AGAAAGTTTC TGCTGTGAGT 
TGGGGTAGGA AGGTGTTGTC ATTTTATAGT TTGTTGTTAG GAGCAGAAAG AAAAGGCAAA 
AATCTCTCTT CTGGTGTGTA TTGTGAAGTT GCCAGTGGTT CAGCCAGAAA CACTGAGGAG 
CTTACTGTTC TGGCTATGGT TGCTGAAAAG TTTGGTCATC AGCAATTGGA TTTATTACCA 
GTTGGTGTAT CTCTTGTGCT TCGCCATGCA CTGGACAAAT GTCGTGACTC TCCACCTGAT 
GATTGGCCTG CAACTGCTTA TGTTCTTGTT GGCAGGGAAG ACCTAGCTGC AGCAAAGATG 
GGGACAGTTA GGAAAGAAAA CCTGTTGTGG AACAATGATA ATTTGACATC AATGTCAGTC 
CCTTATATGC TACATCTGCA ACCTGTAATC ATACCAACAA CTGCACCTGA CATACCTGCA 
TCAGAAGTTT TGAATTCTGA TGATTCAGAT TCTGTCTATA AATCTGTTGA GGATGGCATG 
GAACATATCT TCACTTCAAC AACACAACTC CGATTTGGTC ATGATTTGCG CTTGAATGAG 
GTTAGACGCC TCTTATGCTC AGCAAGGCCA GTGGCTATAC AAACACCTAC TAATCCTAGC 
GTGTCGGATC AAGACCTACA TCAGCAACAA TTATGGAATT TTGCACAAAG GACAACTGCT 
TTACCTTTTG GGCGTGGGGC TTTTACTTTA GCTACAACTT ACACTTTGTT GACAGAGGTT 
CTGGTGTTCC CAAAGCTTGT GCTGGCTGGT CGTTTGCCTG CACAGCAGAA TGCCACAGTA 
AATCTTGACC TAAGTAGTAG AAGTGTCTCA GAATTCAAAT CTTGGGCTGA GTTTCATAAT 
GGTGTTGCTG CTGGCCTGAG GCTTGCCCCT TTTCAGGAGA AAATGTTGAG AACTTGGATA 
CAATACAATA GACCTTCTGA ACCAAATTTT ACTCATGCTG GTTTGCTTCT AGCATTTGGC 
TTGCATGAGC ATCTAAGAGT CTTAACTATG ACTGATGCAT ATCGATACCT CTCACAGGAG 
CATGATATAA CAACACTTGG TTTGTTACTT GGTTTGGCAG CATCTCATAG GGGAACAATG 
GATCCTGCAA TATCAAAGAT GCTCTACTTT CATGTTCCTT CTCGACATCC ATCTTCTACT 
CCAGAGCTGG AGTTACCAAC TCTTCTACAG TCAGCAGCAG TAATGGGAAT TGGACTTCTA 
TATGAAGGCT CAGCTCATGC TCTCACCATG AAGATACTTC TGGGTGAGAT TGGCCGAAGA 
AGTGGAGGTG ACAACGTGCT AGAAAGGGAA GGGTATGCCG TTGCTGCCGG TTCTGCATTA 
GGATTTGTTG CCTTGGGTCA TGGCAATTGT GCATTTGAAT TCATGGATAC TTTCCTAGAT 
CGACTTTTTG AATATATTGG CAGTAAAGAA GTCTACCATG AAAAGTACCT AAATGCAACA 
ACTGCCGATG ATCAAAGTTG CAACCCTGGA CAGATGATGG ATGGAACACA AATAAATGTT 
GATGTTACTG CACCTGGAGC AATAATTGCT CTAACCTTGA TATTTTTGAA AGCCGAGTCT 
GAGGAGATTG CAGCTAGACT CAATATCCCC AATACTTACT TTGATTTGCA GTATGTGAGG 
CCTGATTTTG TTATGCTTCG CATTATAGCC AGGAATTTAA TATTGTGGAG CAGAATACAA 
CCTACCAAAG GATGGGTTGA TTCTCAAATT CCTGAAACAG TCAAGTCTGG TGTTTCCAAC 
ATGAGTGAAG GGACCATTGA CAGTGATGAA TTGGATGCTG AAGCTCTTTT TCAAGCTTAT 
GTTAACATAG TGACTGGAGC ATGTATTGCA CTTGGACTCA AGTATGCTGG CAGCAGAAAT 
GGTGATGCCC AAGAGCTACT CTACGCTTAC ACTGTCCATT TTCTAAATGA GATTAAGCAC 
ATACCTGTTG AAACATCAAA TATACTACCA AAGGGATTAC TACAATACGT TGACCGTGGA 
ACTCTTGAAC TCTGCTTGCA TCTTATTGTT CTATCTCTCT CTCTGGTAAT GGCAGGATCT 
GGAAATTTGC AGACTTTCCG CTTACTGCGT TATCTTAGAG GAAGAATTTC TGCAGAAGGA 
CAAGTGAATT ACGGATTGCA GATGGCTGTA AGCTTGGCAA TAGGATTCTT ATTCCTTGGA 
GGTGGGACAC ACACATTTTC AACTCAGAAT AGTGCAATTG CTGCATTATT GATCTCACTT 
TACCCACGTT TACCCACTGG ACCAAATGAC AATCGCTGCC ATCTCCAGGC ATTCAGACAC 
CTATATGTAA TAGCTACAGA GCCTCGATGG GTTCAGACGG TGGACGTCGA CACAGAACTT 
CCAGTGTATT GTCCATTGGA AGTGACAGTT GCTGAAACAG AGTATTATGA TGAGACCAAC 
TATTGCGAGG TGACACCTTG TCTTCTGCCA GAACGCTCAG TGCTTAAAAG CATTCGAGTT 
TGTGGGCCTA GATACTGGCC TCAGGTGATA AAACTTGCAC CTGAAGACAA GCCATGGTGG 
AGATCTGGAG ACAAGACAGA TCCATTCAAT GGGGGGGTAC TCTACATAAA ACGAAAAGTT 
GGATCATGCT CCTACTCTGA CGATCCAATT GGATGCCAAT CCTTGATTTC ACGGGCAATG 
CATGAGGTCT GTGATGCACC ATCTGCTAGT TGCAGTAATC AACCAAACAG CACTAATGAT 
AGCTACTTCA GAGTTGATCA GCTAGTAAGC ACTTTCTCGG CCAATCCAAG CTTGATTGCT 
TTTGCAAAAC TGTGTTCCGT GTCATGGAAG AGCAGATGCA ATAGCAATTT TCAGGAGTTC 
TGCTCTCAAG TGCTGTATGA ATGTATGAGC AAAGATAGAC CATCACTGTT ACAGGTCTAC 
ATCAGTTTTT ACACAACAAT AGAGTTAATG TGGGAGCATT TGAAGACTGG GCATTTTCCT 
TTATATGATT CCCTTTTCCT TCCTAACTTG AAGGTCGCTT TGGCCTACAA CGAAGCATTA 
GTTGATGCTA GAATCACAAA TGGAGGAATT ATTCATTCGA CATTCTTGGA GTCACTTATG 
AAGCGAGTGG GAGACATCTT TGCGGAGCTG CCAAACCTCG AGGACAACCT CCACCGTTAC 
TTGAGCATGG GCACATGGCC TGATACCCAA AACGACATCG TGGTCCTCTC GTGGTATCTC 
CATTGGTACA ATATACCACC TCCCCATGTT GTGGCTTCTG CAGTTGAGAA GGTCAGGCCC 
AGAGTTCCTG CCGGTGTATC TATGCTCCCT CTGCTTCGCC TCCTGCTGCC AAACACGCAC 
CTTGTGGGAC TAATGGAGAT AGAGAAGCTC CACATTGTAA TGGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002439620.2
CDS	164..4774
Translation	MKIQCYLSIFLHINVHSARYGKGNAPSLLLVFLATDIDGMPIISFLLDEQKILLAVRIQVDDTSEEALGDIKPHMSWNIPAFAAVPVVVTRPRVRVGVLPFTDILVLSSDNDLLLYSGKQCLCKYTLPNELGKGFFSNHELNSDISDIYSDLKITSIADAVEGRINVTCSNGLMLRCSLRKNPSSSLVSDCITAMAEGLQSCFYNQFVSLFWGDSDAAYLHSSSQVDSEWEYFSHEIKRVCTKYGQTLSTKSLTLPSKAWDFLINSKYHSQYCKRALSSSNSLLPASYNTCKTVFNPFLQDEHSLDVPYNIRFMRETLETLHALYENLKLNILRKEDLGRLASLLCTVASSLGEHSYVDYYCRDFPLNLIESPSLVSSNSLTTPPSLFRWFEYCLCHGLDSAKLEDIPTLMRKQKVSAVSWGRKVLSFYSLLLGAERKGKNLSSGVYCEVASGSARNTEELTVLAMVAEKFGHQQLDLLPVGVSLVLRHALDKCRDSPPDDWPATAYVLVGREDLAAAKMGTVRKENLLWNNDNLTSMSVPYMLHLQPVIIPTTAPDIPASEVLNSDDSDSVYKSVEDGMEHIFTSTTQLRFGHDLRLNEVRRLLCSARPVAIQTPTNPSVSDQDLHQQQLWNFAQRTTALPFGRGAFTLATTYTLLTEVLVFPKLVLAGRLPAQQNATVNLDLSSRSVSEFKSWAEFHNGVAAGLRLAPFQEKMLRTWIQYNRPSEPNFTHAGLLLAFGLHEHLRVLTMTDAYRYLSQEHDITTLGLLLGLAASHRGTMDPAISKMLYFHVPSRHPSSTPELELPTLLQSAAVMGIGLLYEGSAHALTMKILLGEIGRRSGGDNVLEREGYAVAAGSALGFVALGHGNCAFEFMDTFLDRLFEYIGSKEVYHEKYLNATTADDQSCNPGQMMDGTQINVDVTAPGAIIALTLIFLKAESEEIAARLNIPNTYFDLQYVRPDFVMLRIIARNLILWSRIQPTKGWVDSQIPETVKSGVSNMSEGTIDSDELDAEALFQAYVNIVTGACIALGLKYAGSRNGDAQELLYAYTVHFLNEIKHIPVETSNILPKGLLQYVDRGTLELCLHLIVLSLSLVMAGSGNLQTFRLLRYLRGRISAEGQVNYGLQMAVSLAIGFLFLGGGTHTFSTQNSAIAALLISLYPRLPTGPNDNRCHLQAFRHLYVIATEPRWVQTVDVDTELPVYCPLEVTVAETEYYDETNYCEVTPCLLPERSVLKSIRVCGPRYWPQVIKLAPEDKPWWRSGDKTDPFNGGVLYIKRKVGSCSYSDDPIGCQSLISRAMHEVCDAPSASCSNQPNSTNDSYFRVDQLVSTFSANPSLIAFAKLCSVSWKSRCNSNFQEFCSQVLYECMSKDRPSLLQVYISFYTTIELMWEHLKTGHFPLYDSLFLPNLKVALAYNEALVDARITNGGIIHSTFLESLMKRVGDIFAELPNLEDNLHRYLSMGTWPDTQNDIVVLSWYLHWYNIPPPHVVASAVEKVRPRVPAGVSMLPLLRLLLPNTHLVGLMEIEKLHIVMEY

Target ORF information:

RefSeq Version	XM_002439575.2
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor anaphase-promoting complex subunit 1 (LOC8061339), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002439575.2

ORF Insert Sequence:

ATGAAAATAC AATGCTACCT GTCGATATTT CTTCACATAA ATGTGCATTC CGCAAGATAT 
GGCAAGGGGA ATGCTCCGAG TCTGCTGCTA GTTTTCTTGG CAACTGATAT TGATGGGATG 
CCAATAATTA GTTTTTTACT GGATGAGCAA AAAATATTGC TTGCTGTAAG GATTCAAGTT 
GATGATACTA GTGAAGAAGC TTTGGGTGAT ATTAAGCCTC ACATGAGCTG GAACATTCCT 
GCATTTGCTG CTGTGCCAGT TGTCGTGACT CGTCCAAGAG TACGGGTTGG TGTTCTTCCC 
TTTACAGACA TCCTTGTTCT GAGTTCAGAT AATGATCTAC TTCTCTATTC TGGGAAGCAG 
TGCCTCTGCA AATATACTCT CCCTAATGAA TTGGGAAAGG GTTTCTTTTC AAATCATGAG 
CTAAATTCAG ATATTTCAGA TATTTACTCT GATCTGAAGA TCACAAGCAT AGCTGATGCT 
GTTGAAGGCC GTATAAACGT GACATGCAGT AATGGCCTGA TGCTCAGATG TTCGTTGCGC 
AAAAATCCTT CCTCTTCTTT GGTTAGTGAC TGCATAACTG CAATGGCAGA GGGTTTGCAG 
AGTTGCTTTT ACAATCAGTT TGTTTCTCTT TTTTGGGGTG ATAGTGATGC TGCCTACCTA 
CATTCAAGCT CTCAGGTGGA TTCAGAATGG GAATATTTTA GTCATGAAAT AAAAAGAGTT 
TGTACAAAGT ATGGACAAAC TTTGTCAACT AAATCTCTCA CTTTGCCAAG TAAAGCTTGG 
GACTTCCTGA TCAACAGCAA ATACCATTCC CAATACTGCA AAAGGGCCCT ATCCTCTTCC 
AATTCACTTT TGCCTGCGTC ATATAATACA TGCAAAACTG TCTTTAATCC CTTTCTCCAG 
GATGAGCATA GTTTAGATGT GCCATATAAT ATTCGGTTCA TGAGAGAAAC ATTGGAGACA 
TTGCATGCCC TATATGAGAA CTTGAAGCTT AACATCTTGC GGAAAGAGGA TCTAGGACGT 
CTCGCTTCAT TGTTATGTAC TGTTGCTTCA TCTCTGGGTG AACATAGTTA TGTTGATTAT 
TATTGCCGTG ACTTTCCGCT CAACTTGATT GAATCCCCAT CCCTAGTCTC TTCAAATTCT 
TTAACAACTC CGCCGTCCCT GTTTCGTTGG TTTGAATACT GCCTATGTCA TGGTTTGGAC 
TCGGCAAAGC TGGAAGACAT TCCTACTCTA ATGCGCAAAC AGAAAGTTTC TGCTGTGAGT 
TGGGGTAGGA AGGTGTTGTC ATTTTATAGT TTGTTGTTAG GAGCAGAAAG AAAAGGCAAA 
AATCTCTCTT CTGGTGTGTA TTGTGAAGTT GCCAGTGGTT CAGCCAGAAA CACTGAGGAG 
CTTACTGTTC TGGCTATGGT TGCTGAAAAG TTTGGTCATC AGCAATTGGA TTTATTACCA 
GTTGGTGTAT CTCTTGTGCT TCGCCATGCA CTGGACAAAT GTCGTGACTC TCCACCTGAT 
GATTGGCCTG CAACTGCTTA TGTTCTTGTT GGCAGGGAAG ACCTAGCTGC AGCAAAGATG 
GGGACAGTTA GGAAAGAAAA CCTGTTGTGG AACAATGATA ATTTGACATC AATGTCAGTC 
CCTTATATGC TACATCTGCA ACCTGTAATC ATACCAACAA CTGCACCTGA CATACCTGCA 
TCAGAAGTTT TGAATTCTGA TGATTCAGAT TCTGTCTATA AATCTGTTGA GGATGGCATG 
GAACATATCT TCACTTCAAC AACACAACTC CGATTTGGTC ATGATTTGCG CTTGAATGAG 
GTTAGACGCC TCTTATGCTC AGCAAGGCCA GTGGCTATAC AAACACCTAC TAATCCTAGC 
GTGTCGGATC AAGACCTACA TCAGCAACAA TTATGGAATT TTGCACAAAG GACAACTGCT 
TTACCTTTTG GGCGTGGGGC TTTTACTTTA GCTACAACTT ACACTTTGTT GACAGAGGTT 
CTGGTGTTCC CAAAGCTTGT GCTGGCTGGT CGTTTGCCTG CACAGCAGAA TGCCACAGTA 
AATCTTGACC TAAGTAGTAG AAGTGTCTCA GAATTCAAAT CTTGGGCTGA GTTTCATAAT 
GGTGTTGCTG CTGGCCTGAG GCTTGCCCCT TTTCAGGAGA AAATGTTGAG AACTTGGATA 
CAATACAATA GACCTTCTGA ACCAAATTTT ACTCATGCTG GTTTGCTTCT AGCATTTGGC 
TTGCATGAGC ATCTAAGAGT CTTAACTATG ACTGATGCAT ATCGATACCT CTCACAGGAG 
CATGATATAA CAACACTTGG TTTGTTACTT GGTTTGGCAG CATCTCATAG GGGAACAATG 
GATCCTGCAA TATCAAAGAT GCTCTACTTT CATGTTCCTT CTCGACATCC ATCTTCTACT 
CCAGAGCTGG AGTTACCAAC TCTTCTACAG TCAGCAGCAG TAATGGGAAT TGGACTTCTA 
TATGAAGGCT CAGCTCATGC TCTCACCATG AAGATACTTC TGGGTGAGAT TGGCCGAAGA 
AGTGGAGGTG ACAACGTGCT AGAAAGGGAA GGGTATGCCG TTGCTGCCGG TTCTGCATTA 
GGATTTGTTG CCTTGGGTCA TGGCAATTGT GCATTTGAAT TCATGGATAC TTTCCTAGAT 
CGACTTTTTG AATATATTGG CAGTAAAGAA GTCTACCATG AAAAGTACCT AAATGCAACA 
ACTGCCGATG ATCAAAGTTG CAACCCTGGA CAGATGATGG ATGGAACACA AATAAATGTT 
GATGTTACTG CACCTGGAGC AATAATTGCT CTAACCTTGA TATTTTTGAA AGCCGAGTCT 
GAGGAGATTG CAGCTAGACT CAATATCCCC AATACTTACT TTGATTTGCA GTATGTGAGG 
CCTGATTTTG TTATGCTTCG CATTATAGCC AGGAATTTAA TATTGTGGAG CAGAATACAA 
CCTACCAAAG GATGGGTTGA TTCTCAAATT CCTGAAACAG TCAAGTCTGG TGTTTCCAAC 
ATGAGTGAAG GGACCATTGA CAGTGATGAA TTGGATGCTG AAGCTCTTTT TCAAGCTTAT 
GTTAACATAG TGACTGGAGC ATGTATTGCA CTTGGACTCA AGTATGCTGG CAGCAGAAAT 
GGTGATGCCC AAGAGCTACT CTACGCTTAC ACTGTCCATT TTCTAAATGA GATTAAGCAC 
ATACCTGTTG AAACATCAAA TATACTACCA AAGGGATTAC TACAATACGT TGACCGTGGA 
ACTCTTGAAC TCTGCTTGCA TCTTATTGTT CTATCTCTCT CTCTGGTAAT GGCAGGATCT 
GGAAATTTGC AGACTTTCCG CTTACTGCGT TATCTTAGAG GAAGAATTTC TGCAGAAGGA 
CAAGTGAATT ACGGATTGCA GATGGCTGTA AGCTTGGCAA TAGGATTCTT ATTCCTTGGA 
GGTGGGACAC ACACATTTTC AACTCAGAAT AGTGCAATTG CTGCATTATT GATCTCACTT 
TACCCACGTT TACCCACTGG ACCAAATGAC AATCGCTGCC ATCTCCAGGC ATTCAGACAC 
CTATATGTAA TAGCTACAGA GCCTCGATGG GTTCAGACGG TGGACGTCGA CACAGAACTT 
CCAGTGTATT GTCCATTGGA AGTGACAGTT GCTGAAACAG AGTATTATGA TGAGACCAAC 
TATTGCGAGG TGACACCTTG TCTTCTGCCA GAACGCTCAG TGCTTAAAAG CATTCGAGTT 
TGTGGGCCTA GATACTGGCC TCAGGTGATA AAACTTGCAC CTGAAGACAA GCCATGGTGG 
AGATCTGGAG ACAAGACAGA TCCATTCAAT GGGGGGGTAC TCTACATAAA ACGAAAAGTT 
GGATCATGCT CCTACTCTGA CGATCCAATT GGATGCCAAT CCTTGATTTC ACGGGCAATG 
CATGAGGTCT GTGATGCACC ATCTGCTAGT TGCAGTAATC AACCAAACAG CACTAATGAT 
AGCTACTTCA GAGTTGATCA GCTAGTAAGC ACTTTCTCGG CCAATCCAAG CTTGATTGCT 
TTTGCAAAAC TGTGTTCCGT GTCATGGAAG AGCAGATGCA ATAGCAATTT TCAGGAGTTC 
TGCTCTCAAG TGCTGTATGA ATGTATGAGC AAAGATAGAC CATCACTGTT ACAGGTCTAC 
ATCAGTTTTT ACACAACAAT AGAGTTAATG TGGGAGCATT TGAAGACTGG GCATTTTCCT 
TTATATGATT CCCTTTTCCT TCCTAACTTG AAGGTCGCTT TGGCCTACAA CGAAGCATTA 
GTTGATGCTA GAATCACAAA TGGAGGAATT ATTCATTCGA CATTCTTGGA GTCACTTATG 
AAGCGAGTGG GAGACATCTT TGCGGAGCTG CCAAACCTCG AGGACAACCT CCACCGTTAC 
TTGAGCATGG GCACATGGCC TGATACCCAA AACGACATCG TGGTCCTCTC GTGGTATCTC 
CATTGGTACA ATATACCACC TCCCCATGTT GTGGCTTCTG CAGTTGAGAA GGTCAGGCCC 
AGAGTTCCTG CCGGTGTATC TATGCTCCCT CTGCTTCGCC TCCTGCTGCC AAACACGCAC 
CTTGTGGGAC TAATGGAGAT AGAGAAGCTC CACATTGTAA TGGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Sorghum bicolor genome and the diversification of grasses.
Nature457(7229)551-6(2009 Jan)
Paterson AH,Bowers JE,Bruggmann R,Dubchak I,Grimwood J,Gundlach H,Haberer G,Hellsten U,Mitros T,Poliakov A,Schmutz J,Spannagl M,Tang H,Wang X,Wicker T,Bharti AK,Chapman J,Feltus FA,Gowik U,Grigoriev IV,Lyons E,Maher CA,Martis M,Narechania A,Otillar RP,Penning BW,Salamov AA,Wang Y,Zhang L,Carpita NC,Freeling M,Gingle AR,Hash CT,Keller B,Klein P,Kresovich S,McCann MC,Ming R,Peterson DG,Mehboob-ur-Rahman,Ware D,Westhoff P,Mayer KF,Messing J,Rokhsar DS

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LOC8061339 cDNA ORF clone, Sorghum bicolor(sorghum)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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