DNF3 cDNA ORF clone, Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNF3
Entrez Gene ID	4838464
Full Name	aminophospholipid-translocating ATPase
Gene Type	protein-coding
Organism	Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001384069.1	XP_001384106.2	aminophospholipid-translocating ATPase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis.
Jeffries TW, Grigoriev IV, Grimwood J, Laplaza JM, Aerts A, Salamov A, Schmutz J, Lindquist E, Dehal P, Shapiro H, Jin YS, Passoth V, Richardson PM
Nature biotechnology25(3)319-26(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DNF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSk01534	XM_001384069.1 Latest version!	Scheffersomyces stipitis CBS 6054 aminophospholipid-translocating ATPase (DNF3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNF3 ( XM_001384069.1 ) cDNA ORF clone, Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054) -> XP_001384106.2 Scheffersomyces stipitis CBS 6054 aminophospholipid-translocating ATPase (DNF3), partial mRNA.

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CloneID	OSk01534
Clone ID Related Accession (Same CDS sequence)	XM_001384069.1
Accession Version	XM_001384069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054)
Product	aminophospholipid-translocating ATPase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009044). COMPLETENESS: incomplete on the 5' end.

ATGGCCATTT GTGCGTGGTT TTTTTTTCAG CCAGATACTT TCGGACCAGA TTCTTCTTTG 
ATAAATCATA AGAAATCTCT CCACAACTTT TCTCAAAGAC TCTTCATTCG AATATTAGTG 
ACTGAGAAGA TTACACAACT ACTACGGATG AGTCTACCGC CAGAAACACC GCAGGAACAG 
CCTTCCAGCA CTCTGCAAAC GCGACGCAAG AGGGGACTTT CACTCCGATC CCAGCTCTTT 
TCTAAAGCGA TCTCTGGCCA GAACCAGCCA CTGCAACTGC TGGAACATTC CAGAAACCCT 
TTCAGAGTAA AATTCGATAT AACTGAACCT TCAGATTCTA ATGAAAATGC TGATGGCCAA 
GCGAATCTGG CAAATGGCAG CTATCTTCGT ACAGATAACC CATCTCTTGG AACAAATGAC 
AACACCAGTT ATCAGATAGA ATTGAACACA CTACATCCGG ATACAGCCTC AATTCCTCAG 
ATAAACATCA ATGCACCTTC CCCTGATTCT CGTTCTGAAG ATGCAAATTT GCACGTTCCC 
TACGGGCATT TGAACGACTC AACTCTCATA AGATCTACCA CTCATCTAAC AGTCGATTCC 
AACAGATCTG ATGCCAGCTC GCTGTCCTTC TTAGCCAAAC GTAAAAGAAC CTCTCAACGT 
CGCTATGGTA CGAAAAACAG ATTGGTCAAA AACTTGATAA GGCTCAAGGA CAACATCTTG 
GGCAACAAGA CACTTCCAAG TACTGAGAGG GGCCGAGTGA TTCCAATCAC CACAAACTTC 
AACGAGATCA ATTCCTACTT TCAGGATCAG TACTACAACG AACATACCAA GTGTCTCATC 
GACGAACGTA GTGACGAGCC GTATGAGAAG AACATAGTGA CTTCTTCTAA GTATACTGTC 
TATTCGTTTT TACCTAAACA GTTGAGAGCC CAGTTCTCCA AAATCGCCAA TTGCTATTTC 
ATGGTCGTAG CAATCATGCA AATGATCCCC ACATGGTCTA CAACAGGTCA GTACACTACT 
ATCATCCCGT TGATGATCTT CATGTCAATT TCGATAGCTA GAGAAGGATT TGACGACTGG 
AAAAGACATG GCCACGACAA AGAAGAAAAC AACAAAAAGA CAAAAGTCAT CAAAGAAGAC 
GAAAACTTGA GCAACTTCGA TACCCACTCA ATTACAACCA TATTGACAGA AACAATTTCA 
GTCAGTAATA ACAACATCCT TTCTCCTCAT GGATCACGAG CAGCGAGCCC ATCTAATAAC 
GTCTCTTCCA ATTCATCATT ATCAGATCCA GAGCCACAAA ATGAGACAAA CACAAACTAC 
ACGAATTTGG AGCTTATGAA ACAGTACAAT CTCAAGGAGT ACGCCACGAA GTGGAAACAC 
ATAAAAGTAG GAGACATCAT AAAAGTCTCT GAAAATGAAG CCTTCCCAGC AGATGTCATG 
TTATTAGCTA CGAGTGACAC AGATAACCAA GAAGCATTTA TAGAAACCAT GGATTTAGAT 
GGAGAAACGA ACTTAAAGCT GAAGTGTCCA CATGCTGAGA TCTGTAAGAA GTTCTCTAAT 
GTGACTGGGT TGAAAAACAC AAAGATGTTG ATAACCGTAG AAGACCCCAA CATCGATTTG 
TACAACTTTG AAGGTTCGTT TGAGGTAAAC GACAAAGTGT TTGCTTTGGG GCCAGATAAT 
GTCGTTTATA GAGGTAGCGT TCTTCGAAAC ACAAAGTCGG TTTTAGGATT GGTCATTTTC 
ACAGGTGAAG AGACAAAAAT CAGAATGAAC AATATTAAGA ATCCCAGAAC TAAGGCTCCA 
AAATTGCAGA GAAACATCAA CTACATTGTG ATATTTATGG TGTTTGTAGT GATTTCGTTG 
TCGGCGTTTT CCACCATGGT GCAACGTCTT CTATACGAGA GAAATAGAGA CAAGGCATGG 
TATCTCTTTG ATCTGGATGC TGGTGTTGCA TCTACTCTTA TGGGTTTCAT CATCATGTAT 
AATACATTGA TTCCATTATC GCTTTATGTC ACTATGGAAA TCATCAAGGT GATGCAATTG 
CTATTTCTAC AGTATGATAT AGACATGTAC CACGTAGAGA GCAACACACC CGCAGATGCG 
AAGACAGCAA CCATCTTGGA AGAACTTGGT CAAGTCAGCT ACATATTCAG TGACAAGACA 
GGAACTTTGA CTGATAACCA GATGATTTTC AGGAAGTTCA GTGTCTGTGG AGCAAGCTGG 
TTACATGACT TGGACTTGAT GTTGAACGAA AAGGAAGACC CTAACAAAGT CGCTCCTTTA 
GTACCAATGC CACGAACCAG CATGCATCAC CTGCCACGCC AGTCTGGTCG CAAGGTAGGC 
CCAGATTATC AGCCAAGAAC AAGTACTACT TCTATTGCTA GAGAAAGTTT GGAATTATCC 
TTGGTCAGAT CGAATGTTAC CTGGAAGTCT TCTGCTCAAC CAAACAAGAC GCAGGACATG 
TCCAGTTCTT TGAAATTGAT AAAATACATA CAATCGCATC CACATACTAT CTTCTCCAGA 
AAGGCTAAGA TGTTTCTTTT GAGTATTGCC TTGTGCAATA CTTGTCTTCC TAGAAAAGAC 
AATACTTCGA AAAACTCGTC AGTGCTTACT CTTGATGACA TTGACGGGGT ACAGCAGCAG 
GAGTTAAGAG CAAAGCGAAA TGTGGAAGAC TCTTCTATCT CTTATCAAGC TGCTTCTCCA 
GACGAATTGG CACTTGTTCA AGCATCCAAG GATCTAGGAT TTGTGGTTCT AGATAGGCAG 
AACAATGTGT TGACAATTAA GACTTATCCA GAGGGATTTC AGAAAGAACC CAAATTCGAG 
GAGTATCAAG TTCTCGAAGT TATAGAGTTC TCATCTAGTA GAAAAAGAAT GTCTACTGTA 
GTTAGGTTTC CAGACGGAAG AATTTGTCTT ATTTGTAAAG GTGCCGACAA TGTCATTTTG 
GAGAAGTTGA AGTATGCCGA TATGGCAAAA GAGAAAGCAA AAGAAATCTC ACTCAACACG 
ACAGAAAGAA AAACACAACA GGCTGATATA GTTTTGCTGA ACAGATTTTC CTCAGAACTA 
GAATCGAGAA AATCAATTGG CTCCATTGGT CAACGTTTGA GTTTGAGTGG CGGCGATAGA 
ATTACTTCCA CTATTGACAA CACGCTTCTG AATGCTGAAG ATGAGATGGC TGATATTGCC 
TTAAAGGCAA GAAAGTCATT GCATGTCCAT CAAGCAAAGA AGTATAGCAT GGATCAGGCC 
TCGTTTACTA ATGTAGATCC ACCGTCTCCA GTACTTAACC TGTCCGGGAC TCTGGGAAGC 
AATAGCCAGA TCCCCAACGA CAGGCTACTT CTCAATGATG AATACCTTGT CGAAAAGACA 
TTGGAGCATA TTGAAGAATT TTCCACTGAA GGGCTCAGAA CATTGATGTA CTCGTACAGG 
TGGTTGGATA AAAATGAGTA TGAGGCATGG TCCCAAGAGT ATAGCCTTGC TAGGACTGCT 
TTAAAGGATA GAGCCAGAAA GGTAGAGGAA GTAGGAGCTA AAATCGAACA CAAGTTTGAG 
CTTCTTGGTG CAACTGCTAT TGAAGATAAA TTGCAAGAAG GAGTCAGTGA TACTATAGAC 
AAGTTAAGAA GGGCAGGAAT CAAGATGTGG ATGTTGACAG GAGACAAACG TGAAACTGCT 
ATTAATATTG GTTACTCGTG TAGACTCATT AAGGACTACT CCACTGTTGT TATTCTTTCG 
CATGATGAAG GAATCGATAC GATTATGGAT CGTATCACTT CTGCCAGTCA GGAGATACAA 
GCAGGAAGAG TAGCCCATTG TGTTTTGGTA ATTGATGGTG GAACTTTAGC TGAAATTGAA 
AATGATTCCG CTTTGCTCAC TTTGTTCCTT GATCTATGCA TCCAGGTAGA TTCTACAATC 
TGCTGTCGTG CTTCGCCTTC ACAAAAGGCT AATATGGTTA GTGCTGTGCG AAAATTGAAC 
AACAGGGCTG TGACTTTGGC AATTGGAGAT GGTGCAAACG ATATTGCCAT GATCCAGAGT 
GCTGACATCG GTATTGGTAT CACTGGTAAG GAAGGGCTAC AAGCTGCTCG TTCTGCCGAT 
TACGCCATTG CCCAGTTCCG GTTCTTGCTT AAGTTGTTAT TAGTCAATGG TCGTTACAAC 
TACATACGAA CATCCAAGTT TGTCTTGTGC ACTTTCTACA AAGAATTATT GTTTTATTTG 
ACGCAGTGTG TCTATCAGAG AAACACTTTA TTCTCAGGCT CTTCGATGTA TGAAAGTTGG 
TCGTTGTCGA TGTTCAATAC GTTGTTCACT TCTCTCCCCG TCATCTGTAT TGGTATGTTC 
GACAAAGACT TGAGGCCCTC AACGTTGATA GCAGTACCTG AATTGTATGC AAAGGGAAGA 
TTGTATCAAG CTTTCAATTT GAAGATCTTT GTGTCATGGA TGTTCTTGGC TGCCATTCAA 
AGTGTCGGTA TATCTTTCAT GGCTTACTAT GTATGGGGAT TTACCGCTTT ACGTGACAAT 
ACAACTTTCC CATTGGGTTC TTTGGTGTTT GCTGCTCTTG TTGTAATCAT CAATGCGAAG 
TGTGAGTTCA TTGAAATGCA GAATAGACAA TGGCTAGCCT TCGCTGCATT CTTTATTTCT 
GTTGGTGGAT ACGCCTTGTG GAACTGGCTA ATTATGATGT TGTACAGAAC AAAGGATTCG 
CCAATTTACT TTGTAGCCTA CGGTTTACTA ACATGGGGCC GTGATCAGAG TTGGTGGGCT 
ACTCTTCTTG CACTCATCAC AGTTCCTCTA TTTGCTGATA TACTTTTCAA AGTGTTTAAA 
TTCATGTTTA AGCCAGCAGA TGATGAACTT TTCAGGTTGT TTGAAAAAGA TATCGACATG 
AGAAGGTTCT TTGAACAACA CTCATATAAA GAATTGTATC AGGGATGGAC CTTTTCTAAA 
CAACCGTCCA CACTTTTTGC AAAGTTAAAA AACGTTGCTA AAGGAGTATT GGGAAAGCCA 
GCTATCCAAC AAGAACTTGA GGCATTCGAC AATGGTTCAG AACACCAGTC GGCTTACAAT 
AGAAAGAGGG CAGGAACAAA TCCATTACCT TCTGAACTAC CACCTGGCGG TGAAGGAATT 
GCTATTGTCG GTAATGACTT CGAACAACCT GGACGTGTTG AGGATGGGTA TGAGATCCTC 
CCTAGCGGAA AGAGAGTCAA GATTGTAGCA AAGGGGGTCA GTTGGGGAAG TCGTTTCAGA 
CGCAATGGTT CTTCTGCAGA GAACGAGGAT GTGGATGCTA TTATAGATTC AAGATTGAAC 
GATCTTAGAA AAGAAGAGGA AGGTCAAGTT GGAGATCGAT CACCAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001384106.2
CDS	1..5331
Translation	MAICAWFFFQPDTFGPDSSLINHKKSLHNFSQRLFIRILVTEKITQLLRMSLPPETPQEQPSSTLQTRRKRGLSLRSQLFSKAISGQNQPLQLLEHSRNPFRVKFDITEPSDSNENADGQANLANGSYLRTDNPSLGTNDNTSYQIELNTLHPDTASIPQININAPSPDSRSEDANLHVPYGHLNDSTLIRSTTHLTVDSNRSDASSLSFLAKRKRTSQRRYGTKNRLVKNLIRLKDNILGNKTLPSTERGRVIPITTNFNEINSYFQDQYYNEHTKCLIDERSDEPYEKNIVTSSKYTVYSFLPKQLRAQFSKIANCYFMVVAIMQMIPTWSTTGQYTTIIPLMIFMSISIAREGFDDWKRHGHDKEENNKKTKVIKEDENLSNFDTHSITTILTETISVSNNNILSPHGSRAASPSNNVSSNSSLSDPEPQNETNTNYTNLELMKQYNLKEYATKWKHIKVGDIIKVSENEAFPADVMLLATSDTDNQEAFIETMDLDGETNLKLKCPHAEICKKFSNVTGLKNTKMLITVEDPNIDLYNFEGSFEVNDKVFALGPDNVVYRGSVLRNTKSVLGLVIFTGEETKIRMNNIKNPRTKAPKLQRNINYIVIFMVFVVISLSAFSTMVQRLLYERNRDKAWYLFDLDAGVASTLMGFIIMYNTLIPLSLYVTMEIIKVMQLLFLQYDIDMYHVESNTPADAKTATILEELGQVSYIFSDKTGTLTDNQMIFRKFSVCGASWLHDLDLMLNEKEDPNKVAPLVPMPRTSMHHLPRQSGRKVGPDYQPRTSTTSIARESLELSLVRSNVTWKSSAQPNKTQDMSSSLKLIKYIQSHPHTIFSRKAKMFLLSIALCNTCLPRKDNTSKNSSVLTLDDIDGVQQQELRAKRNVEDSSISYQAASPDELALVQASKDLGFVVLDRQNNVLTIKTYPEGFQKEPKFEEYQVLEVIEFSSSRKRMSTVVRFPDGRICLICKGADNVILEKLKYADMAKEKAKEISLNTTERKTQQADIVLLNRFSSELESRKSIGSIGQRLSLSGGDRITSTIDNTLLNAEDEMADIALKARKSLHVHQAKKYSMDQASFTNVDPPSPVLNLSGTLGSNSQIPNDRLLLNDEYLVEKTLEHIEEFSTEGLRTLMYSYRWLDKNEYEAWSQEYSLARTALKDRARKVEEVGAKIEHKFELLGATAIEDKLQEGVSDTIDKLRRAGIKMWMLTGDKRETAINIGYSCRLIKDYSTVVILSHDEGIDTIMDRITSASQEIQAGRVAHCVLVIDGGTLAEIENDSALLTLFLDLCIQVDSTICCRASPSQKANMVSAVRKLNNRAVTLAIGDGANDIAMIQSADIGIGITGKEGLQAARSADYAIAQFRFLLKLLLVNGRYNYIRTSKFVLCTFYKELLFYLTQCVYQRNTLFSGSSMYESWSLSMFNTLFTSLPVICIGMFDKDLRPSTLIAVPELYAKGRLYQAFNLKIFVSWMFLAAIQSVGISFMAYYVWGFTALRDNTTFPLGSLVFAALVVIINAKCEFIEMQNRQWLAFAAFFISVGGYALWNWLIMMLYRTKDSPIYFVAYGLLTWGRDQSWWATLLALITVPLFADILFKVFKFMFKPADDELFRLFEKDIDMRRFFEQHSYKELYQGWTFSKQPSTLFAKLKNVAKGVLGKPAIQQELEAFDNGSEHQSAYNRKRAGTNPLPSELPPGGEGIAIVGNDFEQPGRVEDGYEILPSGKRVKIVAKGVSWGSRFRRNGSSAENEDVDAIIDSRLNDLRKEEEGQVGDRSPK

Target ORF information:

RefSeq Version	XM_001384069.1
Organism	Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054)
Definition	Scheffersomyces stipitis CBS 6054 aminophospholipid-translocating ATPase (DNF3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001384069.1

ORF Insert Sequence:

ATGGCCATTT GTGCGTGGTT TTTTTTTCAG CCAGATACTT TCGGACCAGA TTCTTCTTTG 
ATAAATCATA AGAAATCTCT CCACAACTTT TCTCAAAGAC TCTTCATTCG AATATTAGTG 
ACTGAGAAGA TTACACAACT ACTACGGATG AGTCTACCGC CAGAAACACC GCAGGAACAG 
CCTTCCAGCA CTCTGCAAAC GCGACGCAAG AGGGGACTTT CACTCCGATC CCAGCTCTTT 
TCTAAAGCGA TCTCTGGCCA GAACCAGCCA CTGCAACTGC TGGAACATTC CAGAAACCCT 
TTCAGAGTAA AATTCGATAT AACTGAACCT TCAGATTCTA ATGAAAATGC TGATGGCCAA 
GCGAATCTGG CAAATGGCAG CTATCTTCGT ACAGATAACC CATCTCTTGG AACAAATGAC 
AACACCAGTT ATCAGATAGA ATTGAACACA CTACATCCGG ATACAGCCTC AATTCCTCAG 
ATAAACATCA ATGCACCTTC CCCTGATTCT CGTTCTGAAG ATGCAAATTT GCACGTTCCC 
TACGGGCATT TGAACGACTC AACTCTCATA AGATCTACCA CTCATCTAAC AGTCGATTCC 
AACAGATCTG ATGCCAGCTC GCTGTCCTTC TTAGCCAAAC GTAAAAGAAC CTCTCAACGT 
CGCTATGGTA CGAAAAACAG ATTGGTCAAA AACTTGATAA GGCTCAAGGA CAACATCTTG 
GGCAACAAGA CACTTCCAAG TACTGAGAGG GGCCGAGTGA TTCCAATCAC CACAAACTTC 
AACGAGATCA ATTCCTACTT TCAGGATCAG TACTACAACG AACATACCAA GTGTCTCATC 
GACGAACGTA GTGACGAGCC GTATGAGAAG AACATAGTGA CTTCTTCTAA GTATACTGTC 
TATTCGTTTT TACCTAAACA GTTGAGAGCC CAGTTCTCCA AAATCGCCAA TTGCTATTTC 
ATGGTCGTAG CAATCATGCA AATGATCCCC ACATGGTCTA CAACAGGTCA GTACACTACT 
ATCATCCCGT TGATGATCTT CATGTCAATT TCGATAGCTA GAGAAGGATT TGACGACTGG 
AAAAGACATG GCCACGACAA AGAAGAAAAC AACAAAAAGA CAAAAGTCAT CAAAGAAGAC 
GAAAACTTGA GCAACTTCGA TACCCACTCA ATTACAACCA TATTGACAGA AACAATTTCA 
GTCAGTAATA ACAACATCCT TTCTCCTCAT GGATCACGAG CAGCGAGCCC ATCTAATAAC 
GTCTCTTCCA ATTCATCATT ATCAGATCCA GAGCCACAAA ATGAGACAAA CACAAACTAC 
ACGAATTTGG AGCTTATGAA ACAGTACAAT CTCAAGGAGT ACGCCACGAA GTGGAAACAC 
ATAAAAGTAG GAGACATCAT AAAAGTCTCT GAAAATGAAG CCTTCCCAGC AGATGTCATG 
TTATTAGCTA CGAGTGACAC AGATAACCAA GAAGCATTTA TAGAAACCAT GGATTTAGAT 
GGAGAAACGA ACTTAAAGCT GAAGTGTCCA CATGCTGAGA TCTGTAAGAA GTTCTCTAAT 
GTGACTGGGT TGAAAAACAC AAAGATGTTG ATAACCGTAG AAGACCCCAA CATCGATTTG 
TACAACTTTG AAGGTTCGTT TGAGGTAAAC GACAAAGTGT TTGCTTTGGG GCCAGATAAT 
GTCGTTTATA GAGGTAGCGT TCTTCGAAAC ACAAAGTCGG TTTTAGGATT GGTCATTTTC 
ACAGGTGAAG AGACAAAAAT CAGAATGAAC AATATTAAGA ATCCCAGAAC TAAGGCTCCA 
AAATTGCAGA GAAACATCAA CTACATTGTG ATATTTATGG TGTTTGTAGT GATTTCGTTG 
TCGGCGTTTT CCACCATGGT GCAACGTCTT CTATACGAGA GAAATAGAGA CAAGGCATGG 
TATCTCTTTG ATCTGGATGC TGGTGTTGCA TCTACTCTTA TGGGTTTCAT CATCATGTAT 
AATACATTGA TTCCATTATC GCTTTATGTC ACTATGGAAA TCATCAAGGT GATGCAATTG 
CTATTTCTAC AGTATGATAT AGACATGTAC CACGTAGAGA GCAACACACC CGCAGATGCG 
AAGACAGCAA CCATCTTGGA AGAACTTGGT CAAGTCAGCT ACATATTCAG TGACAAGACA 
GGAACTTTGA CTGATAACCA GATGATTTTC AGGAAGTTCA GTGTCTGTGG AGCAAGCTGG 
TTACATGACT TGGACTTGAT GTTGAACGAA AAGGAAGACC CTAACAAAGT CGCTCCTTTA 
GTACCAATGC CACGAACCAG CATGCATCAC CTGCCACGCC AGTCTGGTCG CAAGGTAGGC 
CCAGATTATC AGCCAAGAAC AAGTACTACT TCTATTGCTA GAGAAAGTTT GGAATTATCC 
TTGGTCAGAT CGAATGTTAC CTGGAAGTCT TCTGCTCAAC CAAACAAGAC GCAGGACATG 
TCCAGTTCTT TGAAATTGAT AAAATACATA CAATCGCATC CACATACTAT CTTCTCCAGA 
AAGGCTAAGA TGTTTCTTTT GAGTATTGCC TTGTGCAATA CTTGTCTTCC TAGAAAAGAC 
AATACTTCGA AAAACTCGTC AGTGCTTACT CTTGATGACA TTGACGGGGT ACAGCAGCAG 
GAGTTAAGAG CAAAGCGAAA TGTGGAAGAC TCTTCTATCT CTTATCAAGC TGCTTCTCCA 
GACGAATTGG CACTTGTTCA AGCATCCAAG GATCTAGGAT TTGTGGTTCT AGATAGGCAG 
AACAATGTGT TGACAATTAA GACTTATCCA GAGGGATTTC AGAAAGAACC CAAATTCGAG 
GAGTATCAAG TTCTCGAAGT TATAGAGTTC TCATCTAGTA GAAAAAGAAT GTCTACTGTA 
GTTAGGTTTC CAGACGGAAG AATTTGTCTT ATTTGTAAAG GTGCCGACAA TGTCATTTTG 
GAGAAGTTGA AGTATGCCGA TATGGCAAAA GAGAAAGCAA AAGAAATCTC ACTCAACACG 
ACAGAAAGAA AAACACAACA GGCTGATATA GTTTTGCTGA ACAGATTTTC CTCAGAACTA 
GAATCGAGAA AATCAATTGG CTCCATTGGT CAACGTTTGA GTTTGAGTGG CGGCGATAGA 
ATTACTTCCA CTATTGACAA CACGCTTCTG AATGCTGAAG ATGAGATGGC TGATATTGCC 
TTAAAGGCAA GAAAGTCATT GCATGTCCAT CAAGCAAAGA AGTATAGCAT GGATCAGGCC 
TCGTTTACTA ATGTAGATCC ACCGTCTCCA GTACTTAACC TGTCCGGGAC TCTGGGAAGC 
AATAGCCAGA TCCCCAACGA CAGGCTACTT CTCAATGATG AATACCTTGT CGAAAAGACA 
TTGGAGCATA TTGAAGAATT TTCCACTGAA GGGCTCAGAA CATTGATGTA CTCGTACAGG 
TGGTTGGATA AAAATGAGTA TGAGGCATGG TCCCAAGAGT ATAGCCTTGC TAGGACTGCT 
TTAAAGGATA GAGCCAGAAA GGTAGAGGAA GTAGGAGCTA AAATCGAACA CAAGTTTGAG 
CTTCTTGGTG CAACTGCTAT TGAAGATAAA TTGCAAGAAG GAGTCAGTGA TACTATAGAC 
AAGTTAAGAA GGGCAGGAAT CAAGATGTGG ATGTTGACAG GAGACAAACG TGAAACTGCT 
ATTAATATTG GTTACTCGTG TAGACTCATT AAGGACTACT CCACTGTTGT TATTCTTTCG 
CATGATGAAG GAATCGATAC GATTATGGAT CGTATCACTT CTGCCAGTCA GGAGATACAA 
GCAGGAAGAG TAGCCCATTG TGTTTTGGTA ATTGATGGTG GAACTTTAGC TGAAATTGAA 
AATGATTCCG CTTTGCTCAC TTTGTTCCTT GATCTATGCA TCCAGGTAGA TTCTACAATC 
TGCTGTCGTG CTTCGCCTTC ACAAAAGGCT AATATGGTTA GTGCTGTGCG AAAATTGAAC 
AACAGGGCTG TGACTTTGGC AATTGGAGAT GGTGCAAACG ATATTGCCAT GATCCAGAGT 
GCTGACATCG GTATTGGTAT CACTGGTAAG GAAGGGCTAC AAGCTGCTCG TTCTGCCGAT 
TACGCCATTG CCCAGTTCCG GTTCTTGCTT AAGTTGTTAT TAGTCAATGG TCGTTACAAC 
TACATACGAA CATCCAAGTT TGTCTTGTGC ACTTTCTACA AAGAATTATT GTTTTATTTG 
ACGCAGTGTG TCTATCAGAG AAACACTTTA TTCTCAGGCT CTTCGATGTA TGAAAGTTGG 
TCGTTGTCGA TGTTCAATAC GTTGTTCACT TCTCTCCCCG TCATCTGTAT TGGTATGTTC 
GACAAAGACT TGAGGCCCTC AACGTTGATA GCAGTACCTG AATTGTATGC AAAGGGAAGA 
TTGTATCAAG CTTTCAATTT GAAGATCTTT GTGTCATGGA TGTTCTTGGC TGCCATTCAA 
AGTGTCGGTA TATCTTTCAT GGCTTACTAT GTATGGGGAT TTACCGCTTT ACGTGACAAT 
ACAACTTTCC CATTGGGTTC TTTGGTGTTT GCTGCTCTTG TTGTAATCAT CAATGCGAAG 
TGTGAGTTCA TTGAAATGCA GAATAGACAA TGGCTAGCCT TCGCTGCATT CTTTATTTCT 
GTTGGTGGAT ACGCCTTGTG GAACTGGCTA ATTATGATGT TGTACAGAAC AAAGGATTCG 
CCAATTTACT TTGTAGCCTA CGGTTTACTA ACATGGGGCC GTGATCAGAG TTGGTGGGCT 
ACTCTTCTTG CACTCATCAC AGTTCCTCTA TTTGCTGATA TACTTTTCAA AGTGTTTAAA 
TTCATGTTTA AGCCAGCAGA TGATGAACTT TTCAGGTTGT TTGAAAAAGA TATCGACATG 
AGAAGGTTCT TTGAACAACA CTCATATAAA GAATTGTATC AGGGATGGAC CTTTTCTAAA 
CAACCGTCCA CACTTTTTGC AAAGTTAAAA AACGTTGCTA AAGGAGTATT GGGAAAGCCA 
GCTATCCAAC AAGAACTTGA GGCATTCGAC AATGGTTCAG AACACCAGTC GGCTTACAAT 
AGAAAGAGGG CAGGAACAAA TCCATTACCT TCTGAACTAC CACCTGGCGG TGAAGGAATT 
GCTATTGTCG GTAATGACTT CGAACAACCT GGACGTGTTG AGGATGGGTA TGAGATCCTC 
CCTAGCGGAA AGAGAGTCAA GATTGTAGCA AAGGGGGTCA GTTGGGGAAG TCGTTTCAGA 
CGCAATGGTT CTTCTGCAGA GAACGAGGAT GTGGATGCTA TTATAGATTC AAGATTGAAC 
GATCTTAGAA AAGAAGAGGA AGGTCAAGTT GGAGATCGAT CACCAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis.
Nature biotechnology25(3)319-26(2007 Mar)
Jeffries TW,Grigoriev IV,Grimwood J,Laplaza JM,Aerts A,Salamov A,Schmutz J,Lindquist E,Dehal P,Shapiro H,Jin YS,Passoth V,Richardson PM

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DNF3 cDNA ORF clone, Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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