POL2 cDNA ORF clone, Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054)

Gene
Clones
gRNAs

Summary

Gene Symbol	POL2
Entrez Gene ID	4837222
Full Name	DNA-directed DNA polymerase epsilon, catalytic subunit A
Gene Type	protein-coding
Organism	Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001383006.1	XP_001383043.2	DNA-directed DNA polymerase epsilon, catalytic subunit A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis.
Jeffries TW, Grigoriev IV, Grimwood J, Laplaza JM, Aerts A, Salamov A, Schmutz J, Lindquist E, Dehal P, Shapiro H, Jin YS, Passoth V, Richardson PM
Nature biotechnology25(3)319-26(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSk00493	XM_001383006.1 Latest version!	Scheffersomyces stipitis CBS 6054 DNA-directed DNA polymerase epsilon, catalytic subunit A (POL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POL2 ( XM_001383006.1 ) cDNA ORF clone, Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054) -> XP_001383043.2 Scheffersomyces stipitis CBS 6054 DNA-directed DNA polymerase epsilon, catalytic subunit A (POL2), mRNA.

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CloneID	OSk00493
Clone ID Related Accession (Same CDS sequence)	XM_001383006.1
Accession Version	XM_001383006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054)
Product	DNA-directed DNA polymerase epsilon, catalytic subunit A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009042).

ATGTCTACAC GAGGAAGAAA CAGTTTCAAG GGACTGACAT CGGCCCGGTT CGTCAAGAAC 
AAGGTCAATT CGGAGTTCCG CAACCGCCAG CCGAGACAAG ACGCAAGAGC CATCGACCCG 
TATATAGCCA ACAACCCCAT GCTCAACGAT TCCATCACCA AAAAGGAGTT GGTGGCTCGA 
ATAGACGCGT TGGATTCGAT GATGGGCTTT TCCCGTATCG AACATGGCGA AGCTGATGGA 
ATGAAACCGC GTAAGGGCTG GTTGGTGAAT ATGCATGCTA CCACTATTCC CAGTGATGAA 
TATCTCACAG GGTACTCTGG TGTAGACTAC TATTTCTTAG ATGAAGAAGG TGGTTCGTTC 
AAAACCACGT TACAGTTTGA TCCGTATTTC TTTCTAGATC TCATTCCCGG GCACGAGTCG 
GAAGTGGAGG AGTGGTTGAA GAAGTTTTTG GAGGAATGCC ATGTCAAGAA CTTATCCCGA 
GTCATCAAAG AAGACCTCGC CCTTCCCAAC CACTTGGTAG GACTCCAGAA GAACTTGATT 
AAATTAACGT TTCACAATGT CCAGGACTTG TTAGCCGCCA GAAGGTTACT CTCTCCTATT 
ATCAAGGACA ATCAGGCTAA GAAGGGCTCC AGAGATATCT ACAATGTAAT GAACTTCACC 
AACGGCAACA GTAACAACTC AGATTTTAAT GATTCTATAG CTGATCCAGC TACTTTGATA 
GACGACATGA GAGAATATGA CATGCCATAC CATGTTCGGG TATCTATTGA TATCAACGTC 
AGAGTCGGTA AATGGTACGA TGTGTATGTC AAGCATTCAC AAGTAACCTT AGTTGAAGAT 
AAGGATAAGA TCGCATTTGC TGATCCTGTA GTATTAGCTT TCGATATCGA AACCACAAAG 
GCTCCTTTGA AGTTTCCAGA TGCCAAAATC GACCAGATCA TGATGATCTC GTATATGATA 
GACGGAGATG GTTTCTTGAT CACTAACAGA GAAATAATCT CTGAAGACAT TGAAGATTTC 
GAGTATACGC CTAAGCCAGA ATACCCTGGG ATGTTTACGA TTTTTAACGA ACCCGATGAA 
AAGCACGTCC TAATGAGATT CTACGAACAT ATAAGGGAGG CTAAACCAAC CGTGATAGCA 
ACATTTAACG GTGATTTTTT CGATTGGCCC TTCGTAGAAA ATAGAACCAG ATTCCACGAT 
ATGGACATGT TTGATGAAAT TGGCTTCGCA AAAGATAATG AAGGCGAGTA TAAGTCCAAG 
TACTGTGTTC ACATGGACTG TTTCAGATGG GTCAAAAGAG ACTCGTATTT GCCACAAGGG 
TCTCAAGGTT TGAAAGCTGT GACAACAGCA AAGTTGGGTT ATAATCCGAC GGAATTAGAT 
CCAGAGCTAA TGACACCATA TGCTTACGAA CACCCACAGT TACTTTCAGA ATACTCGGTT 
TCCGATGCTG TTGCCACTTA CTACTTGTAC TTCAAGTATG TCCATCCGTT CATCTTTTCC 
TTGTGTACGA TTATTCCTTT GAACCCAGAT GAAGTTTTGA GAAAGGGAAC AGGTACGTTG 
TGTGAGATGT TGTTGTCGGT TCAAGCCTAT GAAAATGGCA TTTTGCTTCC GAACAAGCAT 
TCCGATCCAA TTGAGCGGTT TTACGATGGG CATTTGCTAG AATCTGAAAC TTACGTAGGT 
GGTCATGTAG AGTCTTTGGA AGCTGGAGTT TTTAGGAGTG ACATTCCCTA TGACTTTAAG 
GTCGACCCTA CAGCCATAGA TGAGTTGCTT CATAACTTGC ATAGCTCAAT CAAATTCTGT 
ATCGAAGTAG AAAACAACAA GAAGATGGAA CAAATCACCA ACTTCGACGA GGTTTACAAT 
GATATTAAGT CCAAATTGCT AGCATTGAAA GAAACTCCGA AAAGAAAGGA AGAACCCTTG 
ATATATCATG TGGATGTTGC TTCGATGTAT CCTAATATCA TGACCTCTAA TAGACTACAA 
CCAGATTCCA TGAAATCCGA AGAAGACTGT GCAGCCTGTG ATTTCAACAG ACCAGGCAAG 
AACTGTGATA GAAGACTTCC TTGGTCCTGG AGAGGTGAGT ATTTCCCAGC CGAAATGAAC 
GAATACAATA TGATCAAGCG AACTTTACAG AATGAAACAT TTCCAGCTTC AAGACCTTGG 
CTTCCTGAGA GGTCATTTGA CGAGCTATCC TACACTGAAC AAGCAACCTT GATCAAGAAG 
AGAATCAGTG ATTATTCTAG AAAAGTGTAC CATAGAGTCA AGAAATCGAA GGTAGTTAAT 
AGAGAGGCTA TTATCTGTCA ACGAGAAAAT CCTTTCTATG TCAACACTGT TATTTCTTTC 
AGAGATCGTC GTTATGAATT CAAAGGCTTG GCCAAGGTTT GGAAAGGAAA GGTGTCCAAG 
ATTGATAAAC ATGATTCTAT TGCCAGAGAT GAAGCAAAGA AAATGGTAGT TTTGTATGAT 
TCCTTGCAAT TGGCTCATAA GGTTATTTTG AACTCTTTCT ACGGATATGT GATGAGAAAA 
GGTTCCCGTT GGTACTCAAT GGAAATGGCT GGTGTGACAT GTTTGACTGG TGCCACAATT 
ATTCAAATGG CTAGAACTTT AGTCGAAAGA ATTGGAAGAC CTTTGGAACT AGATACCGAT 
GGTATTTGGT GTATTTTGCC CAAGTCTTTC CCAGAGAATT ACAATTTGAA ATGCAAAGAT 
GGCAAGAAGA TTTTTCTTGA ATATCCCTGT TCCATGTTGA ACTACTTAGT TCATGAACAA 
TTTACTAATC ACCAATACCA GGATCTAGTG GATCCAGCCA CTTTCAAATA CAAAACTAGA 
TCTGACAATT CCATTTTCTT CGAAGTAGAT GGTCCATATA AGGCTATGAT TTTGCCTACT 
TCTAAGGAAG AAGGTAAGGG ATTGAAGAAA AGATACGCTG TGTTCAATGA TGATGGTTCT 
CTTGCTGAAT TAAAGGGATT TGAATTGAAG AGAAGAGGAG AGTTGCAGCT TATCAAGAAC 
TTCCAATCTG ATATTTTCAA ATTGTTCTTA GAAGGATCTA CGCTAGAACA ATGTTACCAG 
GCAGTTGCTA CTGTTGCCAA TAACTGGTTG GATGTATTGG ATACAAAAGG AGGAATGCTT 
GAAGATGAAG ATTTAATCGA ACTTATTTGT GAAAATAGAA GTATGTCCAA AAGTTTGGAA 
GAATACGGAG ACCAAAAATC GACATCTATC ACTACTGCCA AGAGATTGGG AGAGTTTCTT 
GGTGAAGAAA TGGTCAAGGA CGCTGGTTTG GCTACCAAGT ACATTATTAG TGCAAAGCCA 
ATTGGTGCTC CTGTGACAGA AAGAGCTGTT CCAGTGTCTA TTTTCTCCTC AGACAAGAAG 
GAGTTTTATT TAAAGAAATG GTTAAAGGAT CCTTCTCTTC AGAAGTTTGG TCCTAGAGAT 
GTGATTGACT GGGGATATTA CCATGAACGT TTGTCTTCAG TAGTTCAGAA GATTATCACG 
ATTCCGGCAG CATTGCAAGA TGTGAAGAAC CCAGTACCCA GAGTTGCACA TCCAGATTGG 
TTGCAGAAAA AGATCCACAC CAAGGAAGAT TCTAGACAAC AAAGTTCGAT TTCTACTTTT 
TTCGGTCTGG CCAACAAGAA AGAAGTAATG GAGAAGCAGG TCAAGGAAAT TAAAGAGATT 
AAGGATATAG AGGACTTTGG AGATATAGAT ACTGTTGGGG CACCCAAGTC AAGAATCGGA 
AAGGTCGTCT CGAGAAAGAG ACGAGCAGCT AGAAACGCGA TCGCAGAAGC TGAAGAAGAC 
GAACGTAATA GTGCCATCTT GAATGCACCT TGTCCGTCCA TGATAGAAGA CTACGAATGC 
TTCTTACAAT ATCAAAAAGC CAAGTGGAAA GTGCAAGCTG GCAACAGAGA GAGAAGAAAG 
AAGATCTTTG GTCTGAATTC TGAATCGTCT CATAGATCGA CAGTAGGTGG TATGATCCGT 
AAACAGGCAG AGAATGTAGC AGGATCGAAT TGGGAAATTT TGGAATACAA GGCTGACCCA 
AGCAAGCCTG GTGAAATCAA GGTACATATT CTGGCTGTTG GTAAGATTCA TACCTTTACT 
TTCCATATTC CGAAGAAGAT CTATGCAGCT TACAAATCTG AACTTCAACC CAGAAAGCAG 
ATCAGTGGGT GTGAAATTGA AAGGTCCAAC TCGATCTTAC CTAATGGACA TGATGGTTCG 
AACTTGTACA AATTAATAAT GTCAGAAAGT AGATTCAATG AAGAGATCAC TGACGTAGAA 
AGTGCTCTTC AAGATTCTAA TATTCTTGGA CTCTACGAGA CACAAATTAA TTCTGTCGAA 
AGAGCTATCA TTGATTTGGG AAACGTTGTA AAGTTCGATG ATACAAGAGT TGGTTCGTTG 
GGTAAAGGTC TCAAGAATGG TTTCCAGGTT AAAGATTTGA ATAGAGTTGA AGGTGCTGAG 
TATTTGAAAC GATTTGAAAT GGACATTGTC TACATGTTAC ACATCGTGAC AAACAGTTAC 
GAGTTCTTCA CAGTTTTCAA ATCTTGGGAA GCGACTGCAG AAATGTACAT CTTGAAACCT 
TCCGCTGGAG CTCAGGAGTT ACCACCCAAC ATCGACAAGA TCTACAGAGA GATCTACGAA 
ACTAAGAAGG ACAGATTAGG GAAGTTGTAC AATATTCTTC AATATCCTGA TAATATGAAC 
TTTGAGACTC ATTACTACAA TGACAGTTCA AAGTTATTCA AGAAGTTGAA TGGTGCAATT 
GGAAAGTTGC ATGCATCAAG AAGTAACAAG GCATTATTTG CTGTTCAATC TCCATATTCG 
TCTAAGATTC TAAATCTTTT GAGTTCTACA TCTGATTTCC CTGTCGTTAG GATGAATGTT 
AGTGAATTGG CGCTACCAGC AGTTGGATGG CAATCCTTGA TTTCAATGAG AGTTATCAAT 
CACTATTTCG TTTTGGCAAA TTGGATCAAG AATTTAGTAG CCCTTGCCAA GTATGGGAAT 
GTTCCATTGT GCAATTTACA AATCGAAAAT GTGGGCTACT TGATAGATAT TGAATATGCA 
AGAAGATTAA GTCAGAACAA TATTGTTCTC TGGTGGTCTG CCAATCCATT GCCTGATCAT 
GGGGGATTTG AACTTGATAA AGCTCCTGAT TATGAAAATC TTAACTTTCC AACAATCAAC 
AACCCTGAGA TTTATGAAAC AGCTTGTCTA GAAGTAGAAA TTGGAAATTT GACTATCAAC 
ACCTTCCTTA CAAGCTCTCT CATCAATGAG GCTGAAGGTT CAGATCTAGC TGATGATGGA 
GTTGTATTCG ACAAAAACAA TGGTGCATCG ACGATTGCGG AGGATTCCTT TTCTTCGCCT 
GCGTTGTCCA TTTTGAGAGG AATGGTCAAG GAGTGGTGGG ACGATGCATT AAAGAACAAC 
ATTAATGCTG ATTCAATGAT GAACAGCTTG GTTACTTGGG TGCAAAGAAA AGACTCGTTC 
CTTTATGATT ATTCCTTGCA TTACCATATA CATAATTTAA CAACGAAAGC ATTATTACAG 
TTGATTGGTG AATTCAAGAG AATGAATTCC CAGGTTGTCT TTGCTAATAG AAACAAGTTA 
ATCATTCAAA CGACCAAGGT TTCTGTTGAG AATTCGTATG CATTTGGTCA ATACATTCTA 
AAAGCTGCTA GATCAAAGCC TTTGTTTAAT TTTTTGGATT TGAACATCAT CAAGTATTGG 
GATATTTTGG TATGGATGGA TGAGTTCAAC TTTGGTGGCA GGTGTTGTAA ACAAATCACA 
GATGATGATA TTCAAGACTT GATGCCTGTC AATAGCTGGC AAATCAAACA ATTCTTGCCG 
GTTATTTTCA GAGACGAATT TGAGGATTGG ATGGTTGTCT TTTTGGATGC TCTTACTAAA 
TTCAAGAACG ACAAATTAAC GGGTAATACT CAATCTGGAA CTCCTCGTGT TACACAAATC 
GTTCATATCC TCAAGGGCCA GAGAAAACTT GAAGCCAAAG AAGAAGAGGA GGAAGATATC 
TTTAATGGGG TCTTTGAGGT GTTCAGAAAG TCATTGCAAA AGAGAATCGA AAGGATCTAC 
CATCGTCAAA ACGAAGCCAT TCTCAACCCT GAATTCGCAC AAGAGTATGA GTTCCCCAAA 
TTACCAGGTT CCCATTTGCA CATGAAGAAT CCAGCATTGG AGTTGGTCAA GTTTTTATGT 
GCTGTATTCG GGTTGTCGAA GAAGAGAACC ATTGAGGTCA GAGTATTGAG AAAGGAATTG 
TTGTCGATCT TTGATATCAA AGAGTTCAGT AGTGAAGCTA ATTTCAGGAA CCCAAGTACT 
ACATTGAAGG TCCCTCATAT TATCTGTGAC TATTGTAACT TCATCAGAGA TGTGGATTTC 
TGTAGAGACT CAGAAAAGGA TCTCTGGAAC TGTTCCAATT GTCACAGACC ATACAATCGT 
GTTTCCATGG AAGAAGAGGT GATCACACAA TATATTAAGC TCATTACCAA GTTCTACAGT 
CAAGATTTCA AGTGTGCAAA GTGTAATTCG GTTAGAGTCG GCAACTTGAG TTTGTTCTGC 
AAGTGTTCTG GAAAATGGGA GGAGACTATC AGTTATTCGG AAGTCGAGAA GAAATTACAT 
GTTTTTGCCA ATATTGCCAA TTCGTACAAA TTCCAGCTTT TGAAAGGATT AATAGAAGAA 
GCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001383043.2
CDS	220..6888
Translation	MSTRGRNSFKGLTSARFVKNKVNSEFRNRQPRQDARAIDPYIANNPMLNDSITKKELVARIDALDSMMGFSRIEHGEADGMKPRKGWLVNMHATTIPSDEYLTGYSGVDYYFLDEEGGSFKTTLQFDPYFFLDLIPGHESEVEEWLKKFLEECHVKNLSRVIKEDLALPNHLVGLQKNLIKLTFHNVQDLLAARRLLSPIIKDNQAKKGSRDIYNVMNFTNGNSNNSDFNDSIADPATLIDDMREYDMPYHVRVSIDINVRVGKWYDVYVKHSQVTLVEDKDKIAFADPVVLAFDIETTKAPLKFPDAKIDQIMMISYMIDGDGFLITNREIISEDIEDFEYTPKPEYPGMFTIFNEPDEKHVLMRFYEHIREAKPTVIATFNGDFFDWPFVENRTRFHDMDMFDEIGFAKDNEGEYKSKYCVHMDCFRWVKRDSYLPQGSQGLKAVTTAKLGYNPTELDPELMTPYAYEHPQLLSEYSVSDAVATYYLYFKYVHPFIFSLCTIIPLNPDEVLRKGTGTLCEMLLSVQAYENGILLPNKHSDPIERFYDGHLLESETYVGGHVESLEAGVFRSDIPYDFKVDPTAIDELLHNLHSSIKFCIEVENNKKMEQITNFDEVYNDIKSKLLALKETPKRKEEPLIYHVDVASMYPNIMTSNRLQPDSMKSEEDCAACDFNRPGKNCDRRLPWSWRGEYFPAEMNEYNMIKRTLQNETFPASRPWLPERSFDELSYTEQATLIKKRISDYSRKVYHRVKKSKVVNREAIICQRENPFYVNTVISFRDRRYEFKGLAKVWKGKVSKIDKHDSIARDEAKKMVVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLTGATIIQMARTLVERIGRPLELDTDGIWCILPKSFPENYNLKCKDGKKIFLEYPCSMLNYLVHEQFTNHQYQDLVDPATFKYKTRSDNSIFFEVDGPYKAMILPTSKEEGKGLKKRYAVFNDDGSLAELKGFELKRRGELQLIKNFQSDIFKLFLEGSTLEQCYQAVATVANNWLDVLDTKGGMLEDEDLIELICENRSMSKSLEEYGDQKSTSITTAKRLGEFLGEEMVKDAGLATKYIISAKPIGAPVTERAVPVSIFSSDKKEFYLKKWLKDPSLQKFGPRDVIDWGYYHERLSSVVQKIITIPAALQDVKNPVPRVAHPDWLQKKIHTKEDSRQQSSISTFFGLANKKEVMEKQVKEIKEIKDIEDFGDIDTVGAPKSRIGKVVSRKRRAARNAIAEAEEDERNSAILNAPCPSMIEDYECFLQYQKAKWKVQAGNRERRKKIFGLNSESSHRSTVGGMIRKQAENVAGSNWEILEYKADPSKPGEIKVHILAVGKIHTFTFHIPKKIYAAYKSELQPRKQISGCEIERSNSILPNGHDGSNLYKLIMSESRFNEEITDVESALQDSNILGLYETQINSVERAIIDLGNVVKFDDTRVGSLGKGLKNGFQVKDLNRVEGAEYLKRFEMDIVYMLHIVTNSYEFFTVFKSWEATAEMYILKPSAGAQELPPNIDKIYREIYETKKDRLGKLYNILQYPDNMNFETHYYNDSSKLFKKLNGAIGKLHASRSNKALFAVQSPYSSKILNLLSSTSDFPVVRMNVSELALPAVGWQSLISMRVINHYFVLANWIKNLVALAKYGNVPLCNLQIENVGYLIDIEYARRLSQNNIVLWWSANPLPDHGGFELDKAPDYENLNFPTINNPEIYETACLEVEIGNLTINTFLTSSLINEAEGSDLADDGVVFDKNNGASTIAEDSFSSPALSILRGMVKEWWDDALKNNINADSMMNSLVTWVQRKDSFLYDYSLHYHIHNLTTKALLQLIGEFKRMNSQVVFANRNKLIIQTTKVSVENSYAFGQYILKAARSKPLFNFLDLNIIKYWDILVWMDEFNFGGRCCKQITDDDIQDLMPVNSWQIKQFLPVIFRDEFEDWMVVFLDALTKFKNDKLTGNTQSGTPRVTQIVHILKGQRKLEAKEEEEEDIFNGVFEVFRKSLQKRIERIYHRQNEAILNPEFAQEYEFPKLPGSHLHMKNPALELVKFLCAVFGLSKKRTIEVRVLRKELLSIFDIKEFSSEANFRNPSTTLKVPHIICDYCNFIRDVDFCRDSEKDLWNCSNCHRPYNRVSMEEEVITQYIKLITKFYSQDFKCAKCNSVRVGNLSLFCKCSGKWEETISYSEVEKKLHVFANIANSYKFQLLKGLIEEAA

Target ORF information:

RefSeq Version	XM_001383006.1
Organism	Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054)
Definition	Scheffersomyces stipitis CBS 6054 DNA-directed DNA polymerase epsilon, catalytic subunit A (POL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001383006.1

ORF Insert Sequence:

ATGTCTACAC GAGGAAGAAA CAGTTTCAAG GGACTGACAT CGGCCCGGTT CGTCAAGAAC 
AAGGTCAATT CGGAGTTCCG CAACCGCCAG CCGAGACAAG ACGCAAGAGC CATCGACCCG 
TATATAGCCA ACAACCCCAT GCTCAACGAT TCCATCACCA AAAAGGAGTT GGTGGCTCGA 
ATAGACGCGT TGGATTCGAT GATGGGCTTT TCCCGTATCG AACATGGCGA AGCTGATGGA 
ATGAAACCGC GTAAGGGCTG GTTGGTGAAT ATGCATGCTA CCACTATTCC CAGTGATGAA 
TATCTCACAG GGTACTCTGG TGTAGACTAC TATTTCTTAG ATGAAGAAGG TGGTTCGTTC 
AAAACCACGT TACAGTTTGA TCCGTATTTC TTTCTAGATC TCATTCCCGG GCACGAGTCG 
GAAGTGGAGG AGTGGTTGAA GAAGTTTTTG GAGGAATGCC ATGTCAAGAA CTTATCCCGA 
GTCATCAAAG AAGACCTCGC CCTTCCCAAC CACTTGGTAG GACTCCAGAA GAACTTGATT 
AAATTAACGT TTCACAATGT CCAGGACTTG TTAGCCGCCA GAAGGTTACT CTCTCCTATT 
ATCAAGGACA ATCAGGCTAA GAAGGGCTCC AGAGATATCT ACAATGTAAT GAACTTCACC 
AACGGCAACA GTAACAACTC AGATTTTAAT GATTCTATAG CTGATCCAGC TACTTTGATA 
GACGACATGA GAGAATATGA CATGCCATAC CATGTTCGGG TATCTATTGA TATCAACGTC 
AGAGTCGGTA AATGGTACGA TGTGTATGTC AAGCATTCAC AAGTAACCTT AGTTGAAGAT 
AAGGATAAGA TCGCATTTGC TGATCCTGTA GTATTAGCTT TCGATATCGA AACCACAAAG 
GCTCCTTTGA AGTTTCCAGA TGCCAAAATC GACCAGATCA TGATGATCTC GTATATGATA 
GACGGAGATG GTTTCTTGAT CACTAACAGA GAAATAATCT CTGAAGACAT TGAAGATTTC 
GAGTATACGC CTAAGCCAGA ATACCCTGGG ATGTTTACGA TTTTTAACGA ACCCGATGAA 
AAGCACGTCC TAATGAGATT CTACGAACAT ATAAGGGAGG CTAAACCAAC CGTGATAGCA 
ACATTTAACG GTGATTTTTT CGATTGGCCC TTCGTAGAAA ATAGAACCAG ATTCCACGAT 
ATGGACATGT TTGATGAAAT TGGCTTCGCA AAAGATAATG AAGGCGAGTA TAAGTCCAAG 
TACTGTGTTC ACATGGACTG TTTCAGATGG GTCAAAAGAG ACTCGTATTT GCCACAAGGG 
TCTCAAGGTT TGAAAGCTGT GACAACAGCA AAGTTGGGTT ATAATCCGAC GGAATTAGAT 
CCAGAGCTAA TGACACCATA TGCTTACGAA CACCCACAGT TACTTTCAGA ATACTCGGTT 
TCCGATGCTG TTGCCACTTA CTACTTGTAC TTCAAGTATG TCCATCCGTT CATCTTTTCC 
TTGTGTACGA TTATTCCTTT GAACCCAGAT GAAGTTTTGA GAAAGGGAAC AGGTACGTTG 
TGTGAGATGT TGTTGTCGGT TCAAGCCTAT GAAAATGGCA TTTTGCTTCC GAACAAGCAT 
TCCGATCCAA TTGAGCGGTT TTACGATGGG CATTTGCTAG AATCTGAAAC TTACGTAGGT 
GGTCATGTAG AGTCTTTGGA AGCTGGAGTT TTTAGGAGTG ACATTCCCTA TGACTTTAAG 
GTCGACCCTA CAGCCATAGA TGAGTTGCTT CATAACTTGC ATAGCTCAAT CAAATTCTGT 
ATCGAAGTAG AAAACAACAA GAAGATGGAA CAAATCACCA ACTTCGACGA GGTTTACAAT 
GATATTAAGT CCAAATTGCT AGCATTGAAA GAAACTCCGA AAAGAAAGGA AGAACCCTTG 
ATATATCATG TGGATGTTGC TTCGATGTAT CCTAATATCA TGACCTCTAA TAGACTACAA 
CCAGATTCCA TGAAATCCGA AGAAGACTGT GCAGCCTGTG ATTTCAACAG ACCAGGCAAG 
AACTGTGATA GAAGACTTCC TTGGTCCTGG AGAGGTGAGT ATTTCCCAGC CGAAATGAAC 
GAATACAATA TGATCAAGCG AACTTTACAG AATGAAACAT TTCCAGCTTC AAGACCTTGG 
CTTCCTGAGA GGTCATTTGA CGAGCTATCC TACACTGAAC AAGCAACCTT GATCAAGAAG 
AGAATCAGTG ATTATTCTAG AAAAGTGTAC CATAGAGTCA AGAAATCGAA GGTAGTTAAT 
AGAGAGGCTA TTATCTGTCA ACGAGAAAAT CCTTTCTATG TCAACACTGT TATTTCTTTC 
AGAGATCGTC GTTATGAATT CAAAGGCTTG GCCAAGGTTT GGAAAGGAAA GGTGTCCAAG 
ATTGATAAAC ATGATTCTAT TGCCAGAGAT GAAGCAAAGA AAATGGTAGT TTTGTATGAT 
TCCTTGCAAT TGGCTCATAA GGTTATTTTG AACTCTTTCT ACGGATATGT GATGAGAAAA 
GGTTCCCGTT GGTACTCAAT GGAAATGGCT GGTGTGACAT GTTTGACTGG TGCCACAATT 
ATTCAAATGG CTAGAACTTT AGTCGAAAGA ATTGGAAGAC CTTTGGAACT AGATACCGAT 
GGTATTTGGT GTATTTTGCC CAAGTCTTTC CCAGAGAATT ACAATTTGAA ATGCAAAGAT 
GGCAAGAAGA TTTTTCTTGA ATATCCCTGT TCCATGTTGA ACTACTTAGT TCATGAACAA 
TTTACTAATC ACCAATACCA GGATCTAGTG GATCCAGCCA CTTTCAAATA CAAAACTAGA 
TCTGACAATT CCATTTTCTT CGAAGTAGAT GGTCCATATA AGGCTATGAT TTTGCCTACT 
TCTAAGGAAG AAGGTAAGGG ATTGAAGAAA AGATACGCTG TGTTCAATGA TGATGGTTCT 
CTTGCTGAAT TAAAGGGATT TGAATTGAAG AGAAGAGGAG AGTTGCAGCT TATCAAGAAC 
TTCCAATCTG ATATTTTCAA ATTGTTCTTA GAAGGATCTA CGCTAGAACA ATGTTACCAG 
GCAGTTGCTA CTGTTGCCAA TAACTGGTTG GATGTATTGG ATACAAAAGG AGGAATGCTT 
GAAGATGAAG ATTTAATCGA ACTTATTTGT GAAAATAGAA GTATGTCCAA AAGTTTGGAA 
GAATACGGAG ACCAAAAATC GACATCTATC ACTACTGCCA AGAGATTGGG AGAGTTTCTT 
GGTGAAGAAA TGGTCAAGGA CGCTGGTTTG GCTACCAAGT ACATTATTAG TGCAAAGCCA 
ATTGGTGCTC CTGTGACAGA AAGAGCTGTT CCAGTGTCTA TTTTCTCCTC AGACAAGAAG 
GAGTTTTATT TAAAGAAATG GTTAAAGGAT CCTTCTCTTC AGAAGTTTGG TCCTAGAGAT 
GTGATTGACT GGGGATATTA CCATGAACGT TTGTCTTCAG TAGTTCAGAA GATTATCACG 
ATTCCGGCAG CATTGCAAGA TGTGAAGAAC CCAGTACCCA GAGTTGCACA TCCAGATTGG 
TTGCAGAAAA AGATCCACAC CAAGGAAGAT TCTAGACAAC AAAGTTCGAT TTCTACTTTT 
TTCGGTCTGG CCAACAAGAA AGAAGTAATG GAGAAGCAGG TCAAGGAAAT TAAAGAGATT 
AAGGATATAG AGGACTTTGG AGATATAGAT ACTGTTGGGG CACCCAAGTC AAGAATCGGA 
AAGGTCGTCT CGAGAAAGAG ACGAGCAGCT AGAAACGCGA TCGCAGAAGC TGAAGAAGAC 
GAACGTAATA GTGCCATCTT GAATGCACCT TGTCCGTCCA TGATAGAAGA CTACGAATGC 
TTCTTACAAT ATCAAAAAGC CAAGTGGAAA GTGCAAGCTG GCAACAGAGA GAGAAGAAAG 
AAGATCTTTG GTCTGAATTC TGAATCGTCT CATAGATCGA CAGTAGGTGG TATGATCCGT 
AAACAGGCAG AGAATGTAGC AGGATCGAAT TGGGAAATTT TGGAATACAA GGCTGACCCA 
AGCAAGCCTG GTGAAATCAA GGTACATATT CTGGCTGTTG GTAAGATTCA TACCTTTACT 
TTCCATATTC CGAAGAAGAT CTATGCAGCT TACAAATCTG AACTTCAACC CAGAAAGCAG 
ATCAGTGGGT GTGAAATTGA AAGGTCCAAC TCGATCTTAC CTAATGGACA TGATGGTTCG 
AACTTGTACA AATTAATAAT GTCAGAAAGT AGATTCAATG AAGAGATCAC TGACGTAGAA 
AGTGCTCTTC AAGATTCTAA TATTCTTGGA CTCTACGAGA CACAAATTAA TTCTGTCGAA 
AGAGCTATCA TTGATTTGGG AAACGTTGTA AAGTTCGATG ATACAAGAGT TGGTTCGTTG 
GGTAAAGGTC TCAAGAATGG TTTCCAGGTT AAAGATTTGA ATAGAGTTGA AGGTGCTGAG 
TATTTGAAAC GATTTGAAAT GGACATTGTC TACATGTTAC ACATCGTGAC AAACAGTTAC 
GAGTTCTTCA CAGTTTTCAA ATCTTGGGAA GCGACTGCAG AAATGTACAT CTTGAAACCT 
TCCGCTGGAG CTCAGGAGTT ACCACCCAAC ATCGACAAGA TCTACAGAGA GATCTACGAA 
ACTAAGAAGG ACAGATTAGG GAAGTTGTAC AATATTCTTC AATATCCTGA TAATATGAAC 
TTTGAGACTC ATTACTACAA TGACAGTTCA AAGTTATTCA AGAAGTTGAA TGGTGCAATT 
GGAAAGTTGC ATGCATCAAG AAGTAACAAG GCATTATTTG CTGTTCAATC TCCATATTCG 
TCTAAGATTC TAAATCTTTT GAGTTCTACA TCTGATTTCC CTGTCGTTAG GATGAATGTT 
AGTGAATTGG CGCTACCAGC AGTTGGATGG CAATCCTTGA TTTCAATGAG AGTTATCAAT 
CACTATTTCG TTTTGGCAAA TTGGATCAAG AATTTAGTAG CCCTTGCCAA GTATGGGAAT 
GTTCCATTGT GCAATTTACA AATCGAAAAT GTGGGCTACT TGATAGATAT TGAATATGCA 
AGAAGATTAA GTCAGAACAA TATTGTTCTC TGGTGGTCTG CCAATCCATT GCCTGATCAT 
GGGGGATTTG AACTTGATAA AGCTCCTGAT TATGAAAATC TTAACTTTCC AACAATCAAC 
AACCCTGAGA TTTATGAAAC AGCTTGTCTA GAAGTAGAAA TTGGAAATTT GACTATCAAC 
ACCTTCCTTA CAAGCTCTCT CATCAATGAG GCTGAAGGTT CAGATCTAGC TGATGATGGA 
GTTGTATTCG ACAAAAACAA TGGTGCATCG ACGATTGCGG AGGATTCCTT TTCTTCGCCT 
GCGTTGTCCA TTTTGAGAGG AATGGTCAAG GAGTGGTGGG ACGATGCATT AAAGAACAAC 
ATTAATGCTG ATTCAATGAT GAACAGCTTG GTTACTTGGG TGCAAAGAAA AGACTCGTTC 
CTTTATGATT ATTCCTTGCA TTACCATATA CATAATTTAA CAACGAAAGC ATTATTACAG 
TTGATTGGTG AATTCAAGAG AATGAATTCC CAGGTTGTCT TTGCTAATAG AAACAAGTTA 
ATCATTCAAA CGACCAAGGT TTCTGTTGAG AATTCGTATG CATTTGGTCA ATACATTCTA 
AAAGCTGCTA GATCAAAGCC TTTGTTTAAT TTTTTGGATT TGAACATCAT CAAGTATTGG 
GATATTTTGG TATGGATGGA TGAGTTCAAC TTTGGTGGCA GGTGTTGTAA ACAAATCACA 
GATGATGATA TTCAAGACTT GATGCCTGTC AATAGCTGGC AAATCAAACA ATTCTTGCCG 
GTTATTTTCA GAGACGAATT TGAGGATTGG ATGGTTGTCT TTTTGGATGC TCTTACTAAA 
TTCAAGAACG ACAAATTAAC GGGTAATACT CAATCTGGAA CTCCTCGTGT TACACAAATC 
GTTCATATCC TCAAGGGCCA GAGAAAACTT GAAGCCAAAG AAGAAGAGGA GGAAGATATC 
TTTAATGGGG TCTTTGAGGT GTTCAGAAAG TCATTGCAAA AGAGAATCGA AAGGATCTAC 
CATCGTCAAA ACGAAGCCAT TCTCAACCCT GAATTCGCAC AAGAGTATGA GTTCCCCAAA 
TTACCAGGTT CCCATTTGCA CATGAAGAAT CCAGCATTGG AGTTGGTCAA GTTTTTATGT 
GCTGTATTCG GGTTGTCGAA GAAGAGAACC ATTGAGGTCA GAGTATTGAG AAAGGAATTG 
TTGTCGATCT TTGATATCAA AGAGTTCAGT AGTGAAGCTA ATTTCAGGAA CCCAAGTACT 
ACATTGAAGG TCCCTCATAT TATCTGTGAC TATTGTAACT TCATCAGAGA TGTGGATTTC 
TGTAGAGACT CAGAAAAGGA TCTCTGGAAC TGTTCCAATT GTCACAGACC ATACAATCGT 
GTTTCCATGG AAGAAGAGGT GATCACACAA TATATTAAGC TCATTACCAA GTTCTACAGT 
CAAGATTTCA AGTGTGCAAA GTGTAATTCG GTTAGAGTCG GCAACTTGAG TTTGTTCTGC 
AAGTGTTCTG GAAAATGGGA GGAGACTATC AGTTATTCGG AAGTCGAGAA GAAATTACAT 
GTTTTTGCCA ATATTGCCAA TTCGTACAAA TTCCAGCTTT TGAAAGGATT AATAGAAGAA 
GCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis.
Nature biotechnology25(3)319-26(2007 Mar)
Jeffries TW,Grigoriev IV,Grimwood J,Laplaza JM,Aerts A,Salamov A,Schmutz J,Lindquist E,Dehal P,Shapiro H,Jin YS,Passoth V,Richardson PM

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POL2 cDNA ORF clone, Scheffersomyces stipitis CBS 6054(Pichia stipitis CBS 6054)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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