ANAPC1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANAPC1
Entrez Gene ID	100932748
Full Name	anaphase promoting complex subunit 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031950991.1	XP_031806851.1	anaphase-promoting complex subunit 1 isoform X1
XM_031950992.1	XP_031806852.1	anaphase-promoting complex subunit 1 isoform X1
XM_012540088.1	XP_012395542.1	anaphase-promoting complex subunit 1
XM_023494825.1	XP_023350593.1	anaphase-promoting complex subunit 1
XM_031950993.1	XP_031806853.1	anaphase-promoting complex subunit 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following ANAPC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANAPC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa119834	XM_012540088.1 Latest version!	Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa150761	XM_031950993.1 Latest version!	Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa150761	XM_023494825.1 Latest version!	Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa224312	XM_031950992.1 Latest version!	Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa224312	XM_031950991.1 Latest version!	Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANAPC1 ( XM_012540088.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012395542.1 Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), mRNA.

Price & Availability↑

CloneID	OSa119834
Clone ID Related Accession (Same CDS sequence)	XM_012540088.1
Accession Version	XM_012540088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	anaphase-promoting complex subunit 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCGAACA TCTATGAAGA GAAGGTAACG ATGATTGCAG CAGGGGATTT GCAGGAGTTT 
GTTCCTTTTG GCCGTGATCA CTGCAAGCAC CACCCTAATG CGCTGAATCT TCAACTTCGG 
CAGCTGCAGC CTGCTTCTGA GCTGTGGTCT TCTGATGGAG CTGCTGGTTT GGTGGGATCC 
CTTCAGGAGG TTACAATCCA TGAAAAACAG AAGGAAAGCT GGCATTTAAG AAAAGGTATA 
AGTGATGTTG GAGAAGAAGT TGAATATGAT GAAGAATTGT ATGTGGCTGG TAATATGGTC 
ATCTGGAGCA AAGGAAGTAA AAGTCAGGCT TCTACTGTTT ATAAAGCTTT TACAGTGGAT 
AGTCCTGTTC TTCAGGCACT CTGGTGTGAC TTCATTATAT CACAGGATAA ATCTGAAAAA 
TATGATAATA GCAATGAAGT AGAAAAATGC ATATGCATAT TACAAAGTTC TTGCATAAAT 
GTGCATAGCA TGGAGGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTC 
TGGCCTACAA AATATGGTTT ATTATTTGAA AGAAGCAGTT CTGCACATGA AGTACCGCCA 
AGTCCACCCA GAGAACCTTT ACCCACTATG TTCAGTATGC TACATCCACT GGATGAAATA 
ACCCCTGTTG TTTGTAAATC TGGAGGTCTT TTTGGAACAT CAAGGGTACA GTATGTTGCT 
GATAGTGCAA TGAAAATTGT TTTTCTAAAT GCTGAGCCTT CTATCATAAT GACATATGAT 
GCTATTCAGG GTTTACATTC TCTCTGGATT CTGCGCAGAG TGAAACCTGA GGAACAGAAT 
GTGGTTTTAA AGTTCTCAGA GCAAGGAGGT ACCCCACAGC ATGTGGCTAC CAGCAGTTCC 
CTTACTGCCC ATCTCAGAAG TCTCTCAAAA GGAGATTCAC CTGTAACTTC ACCTTTTCAG 
AATTATTCTT CCATTCACAG TCAGTCTCGG TCAACATCCT CGCCCAGTTT ACATTCTCGT 
TCACCTTCTA TTTCTAACAT GGCAGCTCTT AGTCGTTCTC ATTCTCCTGC CTTAGGAGTA 
CACTCATTCT CAGGGGTGCA GAGATTCAAC TTTTCAAGCC ATAATCAATC TCCAAAGAGG 
CAGAGTGTTT CTCATTCTCC AAACAGTACT TCTAATGACT CCTTTCTTGC ACCAGAAGTG 
GAGCCAATTG TTCCAGAACT TTGTATTGAC CACTTATGGA CAGAAGCAAT TACCACTATG 
AGAGAGAAAA ATTCTCCAGC TTCAAAAGTA TTTATTACAT CTGACCTCTG TGGCCAGAAG 
TTTTTGTGTT TTTTAGTAGA ATCCCAACTA CAGCTTCGAT GTGTAAAGTT TCAAGAGAGT 
AATGATAAAA CCCAGTTGAT CTTTGGTTCT GTCACTAACT TACAGGCAAA GGATGCAGCA 
CCAGTAGAGA AAATTAATAC CATGCTGGTC CTAGAAGGTA ATGGAAACTT GGTACTATAC 
ACTGGATTGG TTCGGGTAGG GAAAGTTTTC ATTCCTGGAC TACCAGTTCC TTCTCTGACA 
ATGTCAAACA CAATGCCAAG ACCTAGTACT CCCTTGGACA GTGTTAGTAC TCCTAAGCCT 
CTAAGCAAAC CACTTGGCTC CTTGGATGAG GTAGTACTGC TATCTCCAGT TCCAGAACTG 
AGAGATTCTT CAAAAGTTCA TGATTCGCCT TATATTGATG ACTGTACTTT TCAGCAACTT 
GGAAGTTACA TTCACTCTGT TAGAGATCCT GTCCATAACA GAGTAACACT TGAACTCAGT 
AATGGTTCAA TGGTTCGGAT CACCATTCCT GAAATTGCCA CCTCAGAATT AGTAAAAAAG 
TGTTTACAAG CTGTCAAGTC TATACTGCCA AAAGAGGTAG CTGTGCAGAT GCTTGTCAAA 
TGGTACAATG TTCACAATGC TCCAGGAGGA CCCAGTTATC ATTCAGAATG GAATTTATTC 
GTAACTTGTC TAATGAATAT GATGGGCTAT AACACAGACC GCTTATCATG GATCCGTAAT 
CTTGACTTTG AAGGATCTCT TTCTCCAGTC ATAGCACCCA AAAAAGCAAG GCCTTCTGAC 
ACTGGATCAG ATGAGGACTG GGAATATTTA CTAAATTCAG AGTACCACCA GAATGTTGAG 
TCTCATCTTT TGACTAGAGC TTTGCATTTG AATCCTTTGG AAGTTTCACT GATGAAGGAG 
GATGTTTTAC ATAATCTTAC TTTGGATTCT TCTACACTTC TCTTCACTCA TATACCTGCT 
ATCTTTTTTG TGCTTCATCT TGTTTATGAA GAATTAAAAT TGAATAGTTT AATGGGAGAA 
GGAATTCGCT CACTTGTTGT TCTTCTAGTT CAGTTGGCAA GGGATTTACA GTTGGAGCCA 
TATTTAGATT ATTACTATAG AGATTATCCA ACACTTGTGA AAACTTCCCG ACAGTCATGC 
ATAATTGATC AAGCCCAAAT AGGACTTATG CATCACCCGT CATTTTTTAC ACCTGAGCCT 
CCAAGTATAT ATCAGTGGCT GAGTTCTTGC CTAAAGGGTG AAGGAGCATC ACCTTATCCT 
TACCTGTCTG GAGTCTGTGA ACGAAGCAAA CTCATAGTGT TGAGTGTTGC ACTCTATATA 
CTTGGTGATG AGAGAGCTGT TTCTAATGAA GCATCACAAT ATTTATGCAA AGTAAACTCA 
GCACCAAAAA AGAGTCAAGT GGACCAAAAT GACAATAGAT TTAGTTTCAG ACATAGTACA 
CCTGTTAATA GCCTCGCTGA AAAATTAGTA CTCTGGATGA CTAATGTAGG CTTTACCTTG 
AGAGATTTGG AGTCTCTTCC TTTTGGAGTT GCTCTTCCTA TCAGAGATGC TATTTATCAC 
TGCCGAGAAC AGCCTGCTTC AGATTGGCCA GAAGCTGTCT GCCTCTTGAT TGGGCGTCAG 
GATCTATCCA AGCAAGCCTG TGAAGGGAAC TTACCAAAAG GGAAATCTTC TGTGGCTCAG 
GTGCTCTCAT CAGATGTGCC TTCAGGAACT GAAGCAGAGG AAGAAGATGA TGGCATGAAT 
GATATGAATC AGGAAGTCAT GTCACTAATA TGGAGTGAAG ATTTAAGAGT CCAGGAGGTT 
CGAAGACTTC TCCAAAGTGC TCATCCTGTT CGAGTCAATG TAGTACAGAT GCCAGAAGTT 
AATGACCATG AATACATTGA AGAAAAAGAA AACAGGTTGC TCCAGTTGTG TCAGCGAACT 
ATGGCACTTC CCTTGGGGCG TGGAATGTTT ACTTTGTTTT CATACCATCC AGTTCCGACA 
GAACCGTTGC CTGTTCCTAA ACTGAATCTA ACTGGACGGG CTCCTCCTAG GAACACAACT 
GTGGACCTTA ACAGTGGAAA TATTGATGTG CCTCCCAACA TGGCATGTTG GGCTAGTTTT 
CACAATGGTG TTGCTGCGGG GTTAAAAATA GCACCTGCTT CCCAGATTGA CTCAGCTTGG 
ATTGTCTACA ATAAACCAAA GAATGCAGAA TTGGCCAATG AATATGCAGG TTTTCTTATG 
GCCTTAGGCT TGAATGGGCA TCTTACAAAG CTAGCTACTC TTAATATACA TGACTATCTG 
ACCAAGGGCC ATGAGATGAC CAGCATTGGA CTGCTTCTTG GTGTTTCTGC TGCTAAGCTA 
GGCACAATGG ATATGTCAAT TACTCGACTT CTCAGCATCC ATATACCTGC TCTCTTACCT 
CCAACTTCAA CAGAACTTGA TGTCCCTCAC AATGTTCAGG TGGCTGCAGT GATAGGCATA 
GGCCTTGTCT ATCAAGGGAC AGCTCACAGG CATACTGCTG AAGTTCTGCT GGCTGAAATT 
GGACGTCCCC CAGGTCCAGA AATGGAATAT TGCACTGACA GGGAATCATA TTCTTTAGCA 
GCTGGTTTGG CACTGGGCAT GGTTTGCTTG GGGCATGGTA GCAACCTGAT AGGCATGTCT 
GATCTCAATG TACCTGAGCA GCTTTATCAG TATATGGTTG GTGGTCACAG GCGCTTCCAA 
GCAGGAATGC ATAGAGAAAA ACATAAATCT CCAAGTTATC AAATTAAAGA AGGTGACACA 
ATCAATGTAG ATGTGACTTG TCCTGGTGCA ACTTTAGCTT TGGCGATGAT CTACTTAAAA 
ACTAATAACA GATCTATTGC TGACTGGCTT CGAGCTCCTG ATACAATGTA TTTGTTGGAT 
TTTGTGAAAC CAGAATTTCT TCTGCTAAGG ACCCTTGCTC GATGCCTGAT TTTATGGGAT 
GACATTTTGC CTAATTCCAA GTGGGTTGAT AGTAATGTTC CACAAATCAT AAGGGAAAAT 
AGTATTTCTC TTAATGACAC TGAAATACCA TCCTCAGAGG ATCTGAACTT GGAAACTCTG 
GCGCAAGCAC ATGTCTATAT AATTGCTGGA GCTTGTTTGT CTCTGGGTTT CCGATTTGCT 
GGTTCAGAAA ACTTAGCAGC ATTTAATTGT TTGTATAAGT TTGCAAAAGA TTTTATGAAG 
TGTTTGTCTG CACCCACAGC TTCAGTGACA GGCCATTATA ACCTGGAAAC TTGCCTAAGT 
GTCGTGCTCC TCTCTCTTGC CATGGTGATG GCTGGCTCTG GAAATCTGAA GGTTCTCCAG 
CTTTGTCGCT TCATGCATAA GAAGACTGGT GGAGAAATGA ACTATGGTTT CCATTTGGCT 
CATCATATGG CTCTTGGACT TCTCTTTTTA GGAGGAGGCA GATATTCCTT GAGTACTTCA 
AATTCTTCAA TTGCTGCTCT TCTTTGTGCA CTTTATCCCC ACTTTCCAGT TCATAGCACT 
GACAATAGGC ACTTCTTTAA GACCATTAAA GTGAAAGGCC CACGATACTG GGAATTGCTT 
ATAGATTTAA GTAAAGGAAT TCATCACTTG AAATCAATTC TTTCCAAGGA TGGAGTTTTA 
TATGTGAAAC TCAGGGCTGG ACAGCTCTCC TACAAAGAAG ATCCGATGGG CTGGCAAAGT 
TTGTTGGCTC AAACAGTCAC TCATAGAAAT TCTGAAGCCA GAGCTTTCAA GCCAGAAGCT 
ATTTCAGCTT TCACCTCTGA TCCAGCACTT TTGTCATTTG CTGAATATTT CTGCAAACCA 
ACTGTGAACT TGGGCCAGAA ACAGGAAATT CTTGATCTCT TTTCTTCAAT ACTTTATGAA 
TGTGTCACGC AGGAGAATCC AGAAATGCTG CCTGCATATA TAGCAATAGA TCAGGCTGTC 
AGAAGACTAG AAAGAAGGGA AATGTCTGAG ACTTTTGAAC TTTGGCAAAT AAAATTGGTA 
TTAGAATTCT TTAGCTCTAG AAGCCACCAG GAGAGAATGC AGAAGATCCC TAGTCGAGGA 
CTTTTTATGA ACTCTGAATT CCTTCCTGTG ATGAAATGCA CTATTGATAA TGCTTTGGAT 
CAATGGTTAC AAGCTGGAGG TGATGTGTGC CTGCATTCCT ATCTAAGTGG CCAGCCCACA 
GAAGAGTCTC ACCTAAGCAT GTTGGCGTGC TTCCTGATTT ATCATTCTGT TCCTACCCCA 
GGGCAGCTGT CCGCTGGAGG ATTAGAAGGA AGCATGAGTT TTTCTGAGCT ACTTCTAAAA 
TTCAAACAAC TGAAGATGCC AGTTCGAGCA TTGCTAAGAT TAGCTCCTTT GCTTCTTGGA 
AGTCCACAAT CAATGGTACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012395542.1
CDS	78..5741
Translation	MSNIYEEKVTMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWHLRKGISDVGEEVEYDEELYVAGNMVIWSKGSKSQASTVYKAFTVDSPVLQALWCDFIISQDKSEKYDNSNEVEKCICILQSSCINVHSMEGKDYIASLPFQVANVWPTKYGLLFERSSSAHEVPPSPPREPLPTMFSMLHPLDEITPVVCKSGGLFGTSRVQYVADSAMKIVFLNAEPSIIMTYDAIQGLHSLWILRRVKPEEQNVVLKFSEQGGTPQHVATSSSLTAHLRSLSKGDSPVTSPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRSHSPALGVHSFSGVQRFNFSSHNQSPKRQSVSHSPNSTSNDSFLAPEVEPIVPELCIDHLWTEAITTMREKNSPASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSVTNLQAKDAAPVEKINTMLVLEGNGNLVLYTGLVRVGKVFIPGLPVPSLTMSNTMPRPSTPLDSVSTPKPLSKPLGSLDEVVLLSPVPELRDSSKVHDSPYIDDCTFQQLGSYIHSVRDPVHNRVTLELSNGSMVRITIPEIATSELVKKCLQAVKSILPKEVAVQMLVKWYNVHNAPGGPSYHSEWNLFVTCLMNMMGYNTDRLSWIRNLDFEGSLSPVIAPKKARPSDTGSDEDWEYLLNSEYHQNVESHLLTRALHLNPLEVSLMKEDVLHNLTLDSSTLLFTHIPAIFFVLHLVYEELKLNSLMGEGIRSLVVLLVQLARDLQLEPYLDYYYRDYPTLVKTSRQSCIIDQAQIGLMHHPSFFTPEPPSIYQWLSSCLKGEGASPYPYLSGVCERSKLIVLSVALYILGDERAVSNEASQYLCKVNSAPKKSQVDQNDNRFSFRHSTPVNSLAEKLVLWMTNVGFTLRDLESLPFGVALPIRDAIYHCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSSVAQVLSSDVPSGTEAEEEDDGMNDMNQEVMSLIWSEDLRVQEVRRLLQSAHPVRVNVVQMPEVNDHEYIEEKENRLLQLCQRTMALPLGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMACWASFHNGVAAGLKIAPASQIDSAWIVYNKPKNAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHNVQVAAVIGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRFQAGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLNDTEIPSSEDLNLETLAQAHVYIIAGACLSLGFRFAGSENLAAFNCLYKFAKDFMKCLSAPTASVTGHYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFMHKKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPVHSTDNRHFFKTIKVKGPRYWELLIDLSKGIHHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVTHRNSEARAFKPEAISAFTSDPALLSFAEYFCKPTVNLGQKQEILDLFSSILYECVTQENPEMLPAYIAIDQAVRRLERREMSETFELWQIKLVLEFFSSRSHQERMQKIPSRGLFMNSEFLPVMKCTIDNALDQWLQAGGDVCLHSYLSGQPTEESHLSMLACFLIYHSVPTPGQLSAGGLEGSMSFSELLLKFKQLKMPVRALLRLAPLLLGSPQSMVL

Target ORF information:

RefSeq Version	XM_012540088.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012540088.1

ORF Insert Sequence:

ATGTCGAACA TCTATGAAGA GAAGGTAACG ATGATTGCAG CAGGGGATTT GCAGGAGTTT 
GTTCCTTTTG GCCGTGATCA CTGCAAGCAC CACCCTAATG CGCTGAATCT TCAACTTCGG 
CAGCTGCAGC CTGCTTCTGA GCTGTGGTCT TCTGATGGAG CTGCTGGTTT GGTGGGATCC 
CTTCAGGAGG TTACAATCCA TGAAAAACAG AAGGAAAGCT GGCATTTAAG AAAAGGTATA 
AGTGATGTTG GAGAAGAAGT TGAATATGAT GAAGAATTGT ATGTGGCTGG TAATATGGTC 
ATCTGGAGCA AAGGAAGTAA AAGTCAGGCT TCTACTGTTT ATAAAGCTTT TACAGTGGAT 
AGTCCTGTTC TTCAGGCACT CTGGTGTGAC TTCATTATAT CACAGGATAA ATCTGAAAAA 
TATGATAATA GCAATGAAGT AGAAAAATGC ATATGCATAT TACAAAGTTC TTGCATAAAT 
GTGCATAGCA TGGAGGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTC 
TGGCCTACAA AATATGGTTT ATTATTTGAA AGAAGCAGTT CTGCACATGA AGTACCGCCA 
AGTCCACCCA GAGAACCTTT ACCCACTATG TTCAGTATGC TACATCCACT GGATGAAATA 
ACCCCTGTTG TTTGTAAATC TGGAGGTCTT TTTGGAACAT CAAGGGTACA GTATGTTGCT 
GATAGTGCAA TGAAAATTGT TTTTCTAAAT GCTGAGCCTT CTATCATAAT GACATATGAT 
GCTATTCAGG GTTTACATTC TCTCTGGATT CTGCGCAGAG TGAAACCTGA GGAACAGAAT 
GTGGTTTTAA AGTTCTCAGA GCAAGGAGGT ACCCCACAGC ATGTGGCTAC CAGCAGTTCC 
CTTACTGCCC ATCTCAGAAG TCTCTCAAAA GGAGATTCAC CTGTAACTTC ACCTTTTCAG 
AATTATTCTT CCATTCACAG TCAGTCTCGG TCAACATCCT CGCCCAGTTT ACATTCTCGT 
TCACCTTCTA TTTCTAACAT GGCAGCTCTT AGTCGTTCTC ATTCTCCTGC CTTAGGAGTA 
CACTCATTCT CAGGGGTGCA GAGATTCAAC TTTTCAAGCC ATAATCAATC TCCAAAGAGG 
CAGAGTGTTT CTCATTCTCC AAACAGTACT TCTAATGACT CCTTTCTTGC ACCAGAAGTG 
GAGCCAATTG TTCCAGAACT TTGTATTGAC CACTTATGGA CAGAAGCAAT TACCACTATG 
AGAGAGAAAA ATTCTCCAGC TTCAAAAGTA TTTATTACAT CTGACCTCTG TGGCCAGAAG 
TTTTTGTGTT TTTTAGTAGA ATCCCAACTA CAGCTTCGAT GTGTAAAGTT TCAAGAGAGT 
AATGATAAAA CCCAGTTGAT CTTTGGTTCT GTCACTAACT TACAGGCAAA GGATGCAGCA 
CCAGTAGAGA AAATTAATAC CATGCTGGTC CTAGAAGGTA ATGGAAACTT GGTACTATAC 
ACTGGATTGG TTCGGGTAGG GAAAGTTTTC ATTCCTGGAC TACCAGTTCC TTCTCTGACA 
ATGTCAAACA CAATGCCAAG ACCTAGTACT CCCTTGGACA GTGTTAGTAC TCCTAAGCCT 
CTAAGCAAAC CACTTGGCTC CTTGGATGAG GTAGTACTGC TATCTCCAGT TCCAGAACTG 
AGAGATTCTT CAAAAGTTCA TGATTCGCCT TATATTGATG ACTGTACTTT TCAGCAACTT 
GGAAGTTACA TTCACTCTGT TAGAGATCCT GTCCATAACA GAGTAACACT TGAACTCAGT 
AATGGTTCAA TGGTTCGGAT CACCATTCCT GAAATTGCCA CCTCAGAATT AGTAAAAAAG 
TGTTTACAAG CTGTCAAGTC TATACTGCCA AAAGAGGTAG CTGTGCAGAT GCTTGTCAAA 
TGGTACAATG TTCACAATGC TCCAGGAGGA CCCAGTTATC ATTCAGAATG GAATTTATTC 
GTAACTTGTC TAATGAATAT GATGGGCTAT AACACAGACC GCTTATCATG GATCCGTAAT 
CTTGACTTTG AAGGATCTCT TTCTCCAGTC ATAGCACCCA AAAAAGCAAG GCCTTCTGAC 
ACTGGATCAG ATGAGGACTG GGAATATTTA CTAAATTCAG AGTACCACCA GAATGTTGAG 
TCTCATCTTT TGACTAGAGC TTTGCATTTG AATCCTTTGG AAGTTTCACT GATGAAGGAG 
GATGTTTTAC ATAATCTTAC TTTGGATTCT TCTACACTTC TCTTCACTCA TATACCTGCT 
ATCTTTTTTG TGCTTCATCT TGTTTATGAA GAATTAAAAT TGAATAGTTT AATGGGAGAA 
GGAATTCGCT CACTTGTTGT TCTTCTAGTT CAGTTGGCAA GGGATTTACA GTTGGAGCCA 
TATTTAGATT ATTACTATAG AGATTATCCA ACACTTGTGA AAACTTCCCG ACAGTCATGC 
ATAATTGATC AAGCCCAAAT AGGACTTATG CATCACCCGT CATTTTTTAC ACCTGAGCCT 
CCAAGTATAT ATCAGTGGCT GAGTTCTTGC CTAAAGGGTG AAGGAGCATC ACCTTATCCT 
TACCTGTCTG GAGTCTGTGA ACGAAGCAAA CTCATAGTGT TGAGTGTTGC ACTCTATATA 
CTTGGTGATG AGAGAGCTGT TTCTAATGAA GCATCACAAT ATTTATGCAA AGTAAACTCA 
GCACCAAAAA AGAGTCAAGT GGACCAAAAT GACAATAGAT TTAGTTTCAG ACATAGTACA 
CCTGTTAATA GCCTCGCTGA AAAATTAGTA CTCTGGATGA CTAATGTAGG CTTTACCTTG 
AGAGATTTGG AGTCTCTTCC TTTTGGAGTT GCTCTTCCTA TCAGAGATGC TATTTATCAC 
TGCCGAGAAC AGCCTGCTTC AGATTGGCCA GAAGCTGTCT GCCTCTTGAT TGGGCGTCAG 
GATCTATCCA AGCAAGCCTG TGAAGGGAAC TTACCAAAAG GGAAATCTTC TGTGGCTCAG 
GTGCTCTCAT CAGATGTGCC TTCAGGAACT GAAGCAGAGG AAGAAGATGA TGGCATGAAT 
GATATGAATC AGGAAGTCAT GTCACTAATA TGGAGTGAAG ATTTAAGAGT CCAGGAGGTT 
CGAAGACTTC TCCAAAGTGC TCATCCTGTT CGAGTCAATG TAGTACAGAT GCCAGAAGTT 
AATGACCATG AATACATTGA AGAAAAAGAA AACAGGTTGC TCCAGTTGTG TCAGCGAACT 
ATGGCACTTC CCTTGGGGCG TGGAATGTTT ACTTTGTTTT CATACCATCC AGTTCCGACA 
GAACCGTTGC CTGTTCCTAA ACTGAATCTA ACTGGACGGG CTCCTCCTAG GAACACAACT 
GTGGACCTTA ACAGTGGAAA TATTGATGTG CCTCCCAACA TGGCATGTTG GGCTAGTTTT 
CACAATGGTG TTGCTGCGGG GTTAAAAATA GCACCTGCTT CCCAGATTGA CTCAGCTTGG 
ATTGTCTACA ATAAACCAAA GAATGCAGAA TTGGCCAATG AATATGCAGG TTTTCTTATG 
GCCTTAGGCT TGAATGGGCA TCTTACAAAG CTAGCTACTC TTAATATACA TGACTATCTG 
ACCAAGGGCC ATGAGATGAC CAGCATTGGA CTGCTTCTTG GTGTTTCTGC TGCTAAGCTA 
GGCACAATGG ATATGTCAAT TACTCGACTT CTCAGCATCC ATATACCTGC TCTCTTACCT 
CCAACTTCAA CAGAACTTGA TGTCCCTCAC AATGTTCAGG TGGCTGCAGT GATAGGCATA 
GGCCTTGTCT ATCAAGGGAC AGCTCACAGG CATACTGCTG AAGTTCTGCT GGCTGAAATT 
GGACGTCCCC CAGGTCCAGA AATGGAATAT TGCACTGACA GGGAATCATA TTCTTTAGCA 
GCTGGTTTGG CACTGGGCAT GGTTTGCTTG GGGCATGGTA GCAACCTGAT AGGCATGTCT 
GATCTCAATG TACCTGAGCA GCTTTATCAG TATATGGTTG GTGGTCACAG GCGCTTCCAA 
GCAGGAATGC ATAGAGAAAA ACATAAATCT CCAAGTTATC AAATTAAAGA AGGTGACACA 
ATCAATGTAG ATGTGACTTG TCCTGGTGCA ACTTTAGCTT TGGCGATGAT CTACTTAAAA 
ACTAATAACA GATCTATTGC TGACTGGCTT CGAGCTCCTG ATACAATGTA TTTGTTGGAT 
TTTGTGAAAC CAGAATTTCT TCTGCTAAGG ACCCTTGCTC GATGCCTGAT TTTATGGGAT 
GACATTTTGC CTAATTCCAA GTGGGTTGAT AGTAATGTTC CACAAATCAT AAGGGAAAAT 
AGTATTTCTC TTAATGACAC TGAAATACCA TCCTCAGAGG ATCTGAACTT GGAAACTCTG 
GCGCAAGCAC ATGTCTATAT AATTGCTGGA GCTTGTTTGT CTCTGGGTTT CCGATTTGCT 
GGTTCAGAAA ACTTAGCAGC ATTTAATTGT TTGTATAAGT TTGCAAAAGA TTTTATGAAG 
TGTTTGTCTG CACCCACAGC TTCAGTGACA GGCCATTATA ACCTGGAAAC TTGCCTAAGT 
GTCGTGCTCC TCTCTCTTGC CATGGTGATG GCTGGCTCTG GAAATCTGAA GGTTCTCCAG 
CTTTGTCGCT TCATGCATAA GAAGACTGGT GGAGAAATGA ACTATGGTTT CCATTTGGCT 
CATCATATGG CTCTTGGACT TCTCTTTTTA GGAGGAGGCA GATATTCCTT GAGTACTTCA 
AATTCTTCAA TTGCTGCTCT TCTTTGTGCA CTTTATCCCC ACTTTCCAGT TCATAGCACT 
GACAATAGGC ACTTCTTTAA GACCATTAAA GTGAAAGGCC CACGATACTG GGAATTGCTT 
ATAGATTTAA GTAAAGGAAT TCATCACTTG AAATCAATTC TTTCCAAGGA TGGAGTTTTA 
TATGTGAAAC TCAGGGCTGG ACAGCTCTCC TACAAAGAAG ATCCGATGGG CTGGCAAAGT 
TTGTTGGCTC AAACAGTCAC TCATAGAAAT TCTGAAGCCA GAGCTTTCAA GCCAGAAGCT 
ATTTCAGCTT TCACCTCTGA TCCAGCACTT TTGTCATTTG CTGAATATTT CTGCAAACCA 
ACTGTGAACT TGGGCCAGAA ACAGGAAATT CTTGATCTCT TTTCTTCAAT ACTTTATGAA 
TGTGTCACGC AGGAGAATCC AGAAATGCTG CCTGCATATA TAGCAATAGA TCAGGCTGTC 
AGAAGACTAG AAAGAAGGGA AATGTCTGAG ACTTTTGAAC TTTGGCAAAT AAAATTGGTA 
TTAGAATTCT TTAGCTCTAG AAGCCACCAG GAGAGAATGC AGAAGATCCC TAGTCGAGGA 
CTTTTTATGA ACTCTGAATT CCTTCCTGTG ATGAAATGCA CTATTGATAA TGCTTTGGAT 
CAATGGTTAC AAGCTGGAGG TGATGTGTGC CTGCATTCCT ATCTAAGTGG CCAGCCCACA 
GAAGAGTCTC ACCTAAGCAT GTTGGCGTGC TTCCTGATTT ATCATTCTGT TCCTACCCCA 
GGGCAGCTGT CCGCTGGAGG ATTAGAAGGA AGCATGAGTT TTTCTGAGCT ACTTCTAAAA 
TTCAAACAAC TGAAGATGCC AGTTCGAGCA TTGCTAAGAT TAGCTCCTTT GCTTCTTGGA 
AGTCCACAAT CAATGGTACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_023494825.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023350593.1 Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), mRNA.

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CloneID	OSa150761
Clone ID Related Accession (Same CDS sequence)	XM_023494825.1 , XM_031950993.1
Accession Version	XM_023494825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	anaphase-promoting complex subunit 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816727.1) and transcript sequences (GEDN01133461.1 and GEDN01149911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 318 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGAACA TCTATGAAGA GAAGGTAACG ATGATTGCAG CAGGGGATTT GCAGGAGTTT 
GTTCCTTTTG GCCGTGATCA CTGCAAGCAC CACCCTAATG CGCTGAATCT TCAACTTCGG 
CAGCTGCAGC CTGCTTCTGA GCTGTGGTCT TCTGATGGAG CTGCTGGTTT GGTGGGATCC 
CTTCAGGAGG TTACAATCCA TGAAAAACAG AAGGAAAGCT GGCATTTAAG AAAAGGTATA 
AGTGATGTTG GAGAAGAAGT TGAATATGAT GAAGAATTGT ATGTGGCTGG TAATATGGTC 
ATCTGGAGCA AAGGAAGTAA AAGTCAGGCT TCTACTGTTT ATAAAGCTTT TACAGTGGAT 
AGTCCTGTTC TTCAGGCACT CTGGTGTGAC TTCATTATAT CACAGGATAA ATCTGAAAAA 
TATGATAATA GCAATGAAGT AGAAAAATGC ATATGCATAT TACAAAGTTC TTGCATAAAT 
GTGCATAGCA TGGAGGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTC 
TGGCCTACAA AATATGGTTT ATTATTTGAA AGAAGCAGTT CTGCACATGA AGTACCGCCA 
AGTCCACCCA GAGAACCTTT ACCCACTATG TTCAGTATGC TACATCCACT GGATGAAATA 
ACCCCTGTTG TTTGTAAATC TGGAGGTCTT TTTGGAACAT CAAGGGTACA GTATGTTGCT 
GATAGTGCAA TGAAAATTGT TTTTCTAAAT GCTGAGCCTT CTATCATAAT GACATATGAT 
GCTATTCAGG GTTTACATTC TCTCTGGATT CTGCGCAGAG TGAAACCTGA GGAACAGAAT 
GTGGTTTTAA AGTTCTCAGA GCAAGGAGGT ACCCCACAGC ATGTGGCTAC CAGCAGTTCC 
CTTACTGCCC ATCTCAGAAG TCTCTCAAAA GGAGATTCAC CTGTAACTTC ACCTTTTCAG 
AATTATTCTT CCATTCACAG TCAGTCTCGG TCAACATCCT CGCCCAGTTT ACATTCTCGT 
TCACCTTCTA TTTCTAACAT GGCAGCTCTT AGTCGTTCTC ATTCTCCTGC CTTAGGAGTA 
CACTCATTCT CAGGGGTGCA GAGATTCAAC TTTTCAAGCC ATAATCAATC TCCAAAGAGG 
CAGAGTGTTT CTCATTCTCC AAACAGTACT TCTAATGACT CCTTTCTTGC ACCAGAAGTG 
GAGCCAATTG TTCCAGAACT TTGTATTGAC CACTTATGGA CAGAAGCAAT TACCACTATG 
AGAGAGAAAA ATTCTCCAGC TTCAAAAGTA TTTATTACAT CTGACCTCTG TGGCCAGAAG 
TTTTTGTGTT TTTTAGTAGA ATCCCAACTA CAGCTTCGAT GTGTAAAGTT TCAAGAGAGT 
AATGATAAAA CCCAGTTGAT CTTTGGTTCT GTCACTAACT TACAGGCAAA GGATGCAGCA 
CCAGTAGAGA AAATTAATAC CATGCTGGTC CTAGAAGGTA ATGGAAACTT GGTACTATAC 
ACTGGATTGG TTCGGGTAGG GAAAGTTTTC ATTCCTGGAC TACCAGTTCC TTCTCTGACA 
ATGTCAAACA CAATGCCAAG ACCTAGTACT CCCTTGGACA GTGTTAGTAC TCCTAAGCCT 
CTAAGCAAAC CACTTGGCTC CTTGGATGAG GTAGTACTGC TATCTCCAGT TCCAGAACTG 
AGAGATTCTT CAAAAGTTCA TGATTCGCCT TATATTGATG ACTGTACTTT TCAGCAACTT 
GGAAGTTACA TTCACTCTGT TAGAGATCCT GTCCATAACA GAGTAACACT TGAACTCAGT 
AATGGTTCAA TGGTTCGGAT CACCATTCCT GAAATTGCCA CCTCAGAATT AGTAAAAAAG 
TGTTTACAAG CTGTCAAGTC TATACTGCCA AAAGAGGTAG CTGTGCAGAT GCTTGTCAAA 
TGGTACAATG TTCACAATGC TCCAGGAGGA CCCAGTTATC ATTCAGAATG GAATTTATTC 
GTAACTTGTC TAATGAATAT GATGGGCTAT AACACAGACC GCTTATCATG GATCCGTAAT 
CTTGACTTTG AAGGATCTCT TTCTCCAGTC ATAGCACCCA AAAAAGCAAG GCCTTCTGAC 
ACTGGATCAG ATGAGGACTG GGAATATTTA CTAAATTCAG AGTACCACCA GAATGTTGAG 
TCTCATCTTT TGACTAGAGC TTTGCATTTG AATCCTTTGG AAGTTTCACT GATGAAGGAG 
GATGTTTTAC ATAATCTTAC TTTGGATTCT TCTACACTTC TCTTCACTCA TATACCTGCT 
ATCTTTTTTG TGCTTCATCT TGTTTATGAA GAATTAAAAT TGAATAGTTT AATGGGAGAA 
GGAATTCGCT CACTTGTTGT TCTTCTAGTT CAGTTGGCAA GGGATTTACA GTTGGAGCCA 
TATTTAGATT ATTACTATAG AGATTATCCA ACACTTGTGA AAACTTCCCG ACAGTCATGC 
ATAATTGATC AAGCCCAAAT AGGACTTATG CATCACCCGT CATTTTTTAC ACCTGAGCCT 
CCAAGTATAT ATCAGTGGCT GAGTTCTTGC CTAAAGGGTG AAGGAGCATC ACCTTATCCT 
TACCTGTCTG GAGTCTGTGA ACGAAGCAAA CTCATAGTGT TGAGTGTTGC ACTCTATATA 
CTTGGTGATG AGAGAGCTGT TTCTAATGAA GCATCACAAT ATTTATGCAA AGTAAACTCA 
GCACCAAAAA AGAGTCAAGT GGACCAAAAT GACAATAGAT TTAGTTTCAG ACATAGTACA 
CCTGTTAATA GCCTCGCTGA AAAATTAGTA CTCTGGATGA CTAATGTAGG CTTTACCTTG 
AGAGATTTGG AGTCTCTTCC TTTTGGAGTT GCTCTTCCTA TCAGAGATGC TATTTATCAC 
TGCCGAGAAC AGCCTGCTTC AGATTGGCCA GAAGCTGTCT GCCTCTTGAT TGGGCGTCAG 
GATCTATCCA AGCAAGCCTG TGAAGGGAAC TTACCAAAAG GGAAATCTTC TGTGGCTCAG 
GTGCTCTCAT CAGATGTGCC TTCAGGAACT GAAGCAGAGG AAGAAGATGA TGGCATGAAT 
GATATGAATC AGGAAGTCAT GTCACTAATA TGGAGTGAAG ATTTAAGAGT CCAGGAGGTT 
CGAAGACTTC TCCAAAGTGC TCATCCTGTT CGAGTCAATG TAGTACAGAT GCCAGAAGTT 
AATGACCATG AATACATTGA AGAAAAAGAA AACAGGTTGC TCCAGTTGTG TCAGCGAACT 
ATGGCACTTC CCTTGGGGCG TGGAATGTTT ACTTTGTTTT CATACCATCC AGTTCCGACA 
GAACCGTTGC CTGTTCCTAA ACTGAATCTA ACTGGACGGG CTCCTCCTAG GAACACAACT 
GTGGACCTTA ACAGTGGAAA TATTGATGTG CCTCCCAACA TGGCATGTTG GGCTAGTTTT 
CACAATGGTG TTGCTGCGGG GTTAAAAATA GCACCTGCTT CCCAGATTGA CTCAGCTTGG 
ATTGTCTACA ATAAACCAAA GAATGCAGAA TTGGCCAATG AATATGCAGG TTTTCTTATG 
GCCTTAGGCT TGAATGGGCA TCTTACAAAG CTAGCTACTC TTAATATACA TGACTATCTG 
ACCAAGGGCC ATGAGATGAC CAGCATTGGA CTGCTTCTTG GTGTTTCTGC TGCTAAGCTA 
GGCACAATGG ATATGTCAAT TACTCGACTT CTCAGCATCC ATATACCTGC TCTCTTACCT 
CCAACTTCAA CAGAACTTGA TGTCCCTCAC AATGTTCAGG TGGCTGCAGT GATAGGCATA 
GGCCTTGTCT ATCAAGGGAC AGCTCACAGG CATACTGCTG AAGTTCTGCT GGCTGAAATT 
GGACGTCCCC CAGGTCCAGA AATGGAATAT TGCACTGACA GGGAATCATA TTCTTTAGCA 
GCTGGTTTGG CACTGGGCAT GGTTTGCTTG GGGCATGGTA GCAACCTGAT AGGCATGTCT 
GATCTCAATG TACCTGAGCA GCTTTATCAG TATATGGTTG GTGGTCACAG GCGCTTCCAA 
GCAGGAATGC ATAGAGAAAA ACATAAATCT CCAAGTTATC AAATTAAAGA AGGTGACACA 
ATCAATGTAG ATGTGACTTG TCCTGGTGCA ACTTTAGCTT TGGCGATGAT CTACTTAAAA 
ACTAATAACA GATCTATTGC TGACTGGCTT CGAGCTCCTG ATACAATGTA TTTGTTGGAT 
TTTGTGAAAC CAGAATTTCT TCTGCTAAGG ACCCTTGCTC GATGCCTGAT TTTATGGGAT 
GACATTTTGC CTAATTCCAA GTGGGTTGAT AGTAATGTTC CACAAATCAT AAGGGAAAAT 
AGTATTTCTC TTAATGACAC TGAAATACCA TCCTCAGAGG ATCTGAACTT GGAAACTCTG 
GCGCAAGCAC ATGTCTATAT AATTGCTGGA GCTTGTTTGT CTCTGGGTTT CCGATTTGCT 
GGTTCAGAAA ACTTAGCAGC ATTTAATTGT TTGTATAAGT TTGCAAAAGA TTTTATGAAG 
TGTTTGTCTG CACCCACAGC TTCAGTGACA GGCCATTATA ACCTGGAAAC TTGCCTAAGT 
GTCGTGCTCC TCTCTCTTGC CATGGTGATG GCTGGCTCTG GAAATCTGAA GGTTCTCCAG 
CTTTGTCGCT TCATGCATAA GAAGACTGGT GGAGAAATGA ACTATGGTTT CCATTTGGCT 
CATCATATGG CTCTTGGACT TCTCTTTTTA GGAGGAGGCA GATATTCCTT GAGTACTTCA 
AATTCTTCAA TTGCTGCTCT TCTTTGTGCA CTTTATCCCC ACTTTCCAGT TCATAGCACT 
GACAATAGGT ATCATCTCCA GGCTCTTCGA CACTTGTATG TACTAGCAGC AGAACCAAGA 
CTTCTTGTTC CTGTTGATGT AGATACAAAT ACACCTTGCT ATGCATTATT AGAGGTTACA 
TACAAGGGTA CTCAGTGGTA TGAAGAAACA ACAGAAGAGC TAATGGCACC TACACTGCTT 
CCTGAGCTTC ACCTTTTAAA ACAGATTAAA GTGAAAGGCC CACGATACTG GGAATTGCTT 
ATAGATTTAA GTAAAGGAAT TCATCACTTG AAATCAATTC TTTCCAAGGA TGGAGTTTTA 
TATGTGAAAC TCAGGGCTGG ACAGCTCTCC TACAAAGAAG ATCCGATGGG CTGGCAAAGT 
TTGTTGGCTC AAACAGTCAC TCATAGAAAT TCTGAAGCCA GAGCTTTCAA GCCAGAAGCT 
ATTTCAGCTT TCACCTCTGA TCCAGCACTT TTGTCATTTG CTGAATATTT CTGCAAACCA 
ACTGTGAACT TGGGCCAGAA ACAGGAAATT CTTGATCTCT TTTCTTCAAT ACTTTATGAA 
TGTGTCACGC AGGAGAATCC AGAAATGCTG CCTGCATATA TAGCAATAGA TCAGGCTGTC 
AGAAGACTAG AAAGAAGGGA AATGTCTGAG ACTTTTGAAC TTTGGCAAAT AAAATTGGTA 
TTAGAATTCT TTAGCTCTAG AAGCCACCAG GAGAGAATGC AGAAGATCCC TAGTCGAGGA 
CTTTTTATGA ACTCTGAATT CCTTCCTGTG ATGAAATGCA CTATTGATAA TGCTTTGGAT 
CAATGGTTAC AAGCTGGAGG TGATGTGTGC CTGCATTCCT ATCTAAGTGG CCAGCCCACA 
GAAGAGTCTC ACCTAAGCAT GTTGGCGTGC TTCCTGATTT ATCATTCTGT TCCTACCCCA 
GGGCAGCTGT CCGCTGGAGG ATTAGAAGGA AGCATGAGTT TTTCTGAGCT ACTTCTAAAA 
TTCAAACAAC TGAAGATGCC AGTTCGAGCA TTGCTAAGAT TAGCTCCTTT GCTTCTTGGA 
AGTCCACAAT CAATGGTACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023350593.1
CDS	558..6401
Translation	MSNIYEEKVTMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWHLRKGISDVGEEVEYDEELYVAGNMVIWSKGSKSQASTVYKAFTVDSPVLQALWCDFIISQDKSEKYDNSNEVEKCICILQSSCINVHSMEGKDYIASLPFQVANVWPTKYGLLFERSSSAHEVPPSPPREPLPTMFSMLHPLDEITPVVCKSGGLFGTSRVQYVADSAMKIVFLNAEPSIIMTYDAIQGLHSLWILRRVKPEEQNVVLKFSEQGGTPQHVATSSSLTAHLRSLSKGDSPVTSPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRSHSPALGVHSFSGVQRFNFSSHNQSPKRQSVSHSPNSTSNDSFLAPEVEPIVPELCIDHLWTEAITTMREKNSPASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSVTNLQAKDAAPVEKINTMLVLEGNGNLVLYTGLVRVGKVFIPGLPVPSLTMSNTMPRPSTPLDSVSTPKPLSKPLGSLDEVVLLSPVPELRDSSKVHDSPYIDDCTFQQLGSYIHSVRDPVHNRVTLELSNGSMVRITIPEIATSELVKKCLQAVKSILPKEVAVQMLVKWYNVHNAPGGPSYHSEWNLFVTCLMNMMGYNTDRLSWIRNLDFEGSLSPVIAPKKARPSDTGSDEDWEYLLNSEYHQNVESHLLTRALHLNPLEVSLMKEDVLHNLTLDSSTLLFTHIPAIFFVLHLVYEELKLNSLMGEGIRSLVVLLVQLARDLQLEPYLDYYYRDYPTLVKTSRQSCIIDQAQIGLMHHPSFFTPEPPSIYQWLSSCLKGEGASPYPYLSGVCERSKLIVLSVALYILGDERAVSNEASQYLCKVNSAPKKSQVDQNDNRFSFRHSTPVNSLAEKLVLWMTNVGFTLRDLESLPFGVALPIRDAIYHCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSSVAQVLSSDVPSGTEAEEEDDGMNDMNQEVMSLIWSEDLRVQEVRRLLQSAHPVRVNVVQMPEVNDHEYIEEKENRLLQLCQRTMALPLGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMACWASFHNGVAAGLKIAPASQIDSAWIVYNKPKNAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHNVQVAAVIGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRFQAGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLNDTEIPSSEDLNLETLAQAHVYIIAGACLSLGFRFAGSENLAAFNCLYKFAKDFMKCLSAPTASVTGHYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFMHKKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPVHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALLEVTYKGTQWYEETTEELMAPTLLPELHLLKQIKVKGPRYWELLIDLSKGIHHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVTHRNSEARAFKPEAISAFTSDPALLSFAEYFCKPTVNLGQKQEILDLFSSILYECVTQENPEMLPAYIAIDQAVRRLERREMSETFELWQIKLVLEFFSSRSHQERMQKIPSRGLFMNSEFLPVMKCTIDNALDQWLQAGGDVCLHSYLSGQPTEESHLSMLACFLIYHSVPTPGQLSAGGLEGSMSFSELLLKFKQLKMPVRALLRLAPLLLGSPQSMVL

Target ORF information:

RefSeq Version	XM_023494825.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494825.1

ORF Insert Sequence:

ATGTCGAACA TCTATGAAGA GAAGGTAACG ATGATTGCAG CAGGGGATTT GCAGGAGTTT 
GTTCCTTTTG GCCGTGATCA CTGCAAGCAC CACCCTAATG CGCTGAATCT TCAACTTCGG 
CAGCTGCAGC CTGCTTCTGA GCTGTGGTCT TCTGATGGAG CTGCTGGTTT GGTGGGATCC 
CTTCAGGAGG TTACAATCCA TGAAAAACAG AAGGAAAGCT GGCATTTAAG AAAAGGTATA 
AGTGATGTTG GAGAAGAAGT TGAATATGAT GAAGAATTGT ATGTGGCTGG TAATATGGTC 
ATCTGGAGCA AAGGAAGTAA AAGTCAGGCT TCTACTGTTT ATAAAGCTTT TACAGTGGAT 
AGTCCTGTTC TTCAGGCACT CTGGTGTGAC TTCATTATAT CACAGGATAA ATCTGAAAAA 
TATGATAATA GCAATGAAGT AGAAAAATGC ATATGCATAT TACAAAGTTC TTGCATAAAT 
GTGCATAGCA TGGAGGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTC 
TGGCCTACAA AATATGGTTT ATTATTTGAA AGAAGCAGTT CTGCACATGA AGTACCGCCA 
AGTCCACCCA GAGAACCTTT ACCCACTATG TTCAGTATGC TACATCCACT GGATGAAATA 
ACCCCTGTTG TTTGTAAATC TGGAGGTCTT TTTGGAACAT CAAGGGTACA GTATGTTGCT 
GATAGTGCAA TGAAAATTGT TTTTCTAAAT GCTGAGCCTT CTATCATAAT GACATATGAT 
GCTATTCAGG GTTTACATTC TCTCTGGATT CTGCGCAGAG TGAAACCTGA GGAACAGAAT 
GTGGTTTTAA AGTTCTCAGA GCAAGGAGGT ACCCCACAGC ATGTGGCTAC CAGCAGTTCC 
CTTACTGCCC ATCTCAGAAG TCTCTCAAAA GGAGATTCAC CTGTAACTTC ACCTTTTCAG 
AATTATTCTT CCATTCACAG TCAGTCTCGG TCAACATCCT CGCCCAGTTT ACATTCTCGT 
TCACCTTCTA TTTCTAACAT GGCAGCTCTT AGTCGTTCTC ATTCTCCTGC CTTAGGAGTA 
CACTCATTCT CAGGGGTGCA GAGATTCAAC TTTTCAAGCC ATAATCAATC TCCAAAGAGG 
CAGAGTGTTT CTCATTCTCC AAACAGTACT TCTAATGACT CCTTTCTTGC ACCAGAAGTG 
GAGCCAATTG TTCCAGAACT TTGTATTGAC CACTTATGGA CAGAAGCAAT TACCACTATG 
AGAGAGAAAA ATTCTCCAGC TTCAAAAGTA TTTATTACAT CTGACCTCTG TGGCCAGAAG 
TTTTTGTGTT TTTTAGTAGA ATCCCAACTA CAGCTTCGAT GTGTAAAGTT TCAAGAGAGT 
AATGATAAAA CCCAGTTGAT CTTTGGTTCT GTCACTAACT TACAGGCAAA GGATGCAGCA 
CCAGTAGAGA AAATTAATAC CATGCTGGTC CTAGAAGGTA ATGGAAACTT GGTACTATAC 
ACTGGATTGG TTCGGGTAGG GAAAGTTTTC ATTCCTGGAC TACCAGTTCC TTCTCTGACA 
ATGTCAAACA CAATGCCAAG ACCTAGTACT CCCTTGGACA GTGTTAGTAC TCCTAAGCCT 
CTAAGCAAAC CACTTGGCTC CTTGGATGAG GTAGTACTGC TATCTCCAGT TCCAGAACTG 
AGAGATTCTT CAAAAGTTCA TGATTCGCCT TATATTGATG ACTGTACTTT TCAGCAACTT 
GGAAGTTACA TTCACTCTGT TAGAGATCCT GTCCATAACA GAGTAACACT TGAACTCAGT 
AATGGTTCAA TGGTTCGGAT CACCATTCCT GAAATTGCCA CCTCAGAATT AGTAAAAAAG 
TGTTTACAAG CTGTCAAGTC TATACTGCCA AAAGAGGTAG CTGTGCAGAT GCTTGTCAAA 
TGGTACAATG TTCACAATGC TCCAGGAGGA CCCAGTTATC ATTCAGAATG GAATTTATTC 
GTAACTTGTC TAATGAATAT GATGGGCTAT AACACAGACC GCTTATCATG GATCCGTAAT 
CTTGACTTTG AAGGATCTCT TTCTCCAGTC ATAGCACCCA AAAAAGCAAG GCCTTCTGAC 
ACTGGATCAG ATGAGGACTG GGAATATTTA CTAAATTCAG AGTACCACCA GAATGTTGAG 
TCTCATCTTT TGACTAGAGC TTTGCATTTG AATCCTTTGG AAGTTTCACT GATGAAGGAG 
GATGTTTTAC ATAATCTTAC TTTGGATTCT TCTACACTTC TCTTCACTCA TATACCTGCT 
ATCTTTTTTG TGCTTCATCT TGTTTATGAA GAATTAAAAT TGAATAGTTT AATGGGAGAA 
GGAATTCGCT CACTTGTTGT TCTTCTAGTT CAGTTGGCAA GGGATTTACA GTTGGAGCCA 
TATTTAGATT ATTACTATAG AGATTATCCA ACACTTGTGA AAACTTCCCG ACAGTCATGC 
ATAATTGATC AAGCCCAAAT AGGACTTATG CATCACCCGT CATTTTTTAC ACCTGAGCCT 
CCAAGTATAT ATCAGTGGCT GAGTTCTTGC CTAAAGGGTG AAGGAGCATC ACCTTATCCT 
TACCTGTCTG GAGTCTGTGA ACGAAGCAAA CTCATAGTGT TGAGTGTTGC ACTCTATATA 
CTTGGTGATG AGAGAGCTGT TTCTAATGAA GCATCACAAT ATTTATGCAA AGTAAACTCA 
GCACCAAAAA AGAGTCAAGT GGACCAAAAT GACAATAGAT TTAGTTTCAG ACATAGTACA 
CCTGTTAATA GCCTCGCTGA AAAATTAGTA CTCTGGATGA CTAATGTAGG CTTTACCTTG 
AGAGATTTGG AGTCTCTTCC TTTTGGAGTT GCTCTTCCTA TCAGAGATGC TATTTATCAC 
TGCCGAGAAC AGCCTGCTTC AGATTGGCCA GAAGCTGTCT GCCTCTTGAT TGGGCGTCAG 
GATCTATCCA AGCAAGCCTG TGAAGGGAAC TTACCAAAAG GGAAATCTTC TGTGGCTCAG 
GTGCTCTCAT CAGATGTGCC TTCAGGAACT GAAGCAGAGG AAGAAGATGA TGGCATGAAT 
GATATGAATC AGGAAGTCAT GTCACTAATA TGGAGTGAAG ATTTAAGAGT CCAGGAGGTT 
CGAAGACTTC TCCAAAGTGC TCATCCTGTT CGAGTCAATG TAGTACAGAT GCCAGAAGTT 
AATGACCATG AATACATTGA AGAAAAAGAA AACAGGTTGC TCCAGTTGTG TCAGCGAACT 
ATGGCACTTC CCTTGGGGCG TGGAATGTTT ACTTTGTTTT CATACCATCC AGTTCCGACA 
GAACCGTTGC CTGTTCCTAA ACTGAATCTA ACTGGACGGG CTCCTCCTAG GAACACAACT 
GTGGACCTTA ACAGTGGAAA TATTGATGTG CCTCCCAACA TGGCATGTTG GGCTAGTTTT 
CACAATGGTG TTGCTGCGGG GTTAAAAATA GCACCTGCTT CCCAGATTGA CTCAGCTTGG 
ATTGTCTACA ATAAACCAAA GAATGCAGAA TTGGCCAATG AATATGCAGG TTTTCTTATG 
GCCTTAGGCT TGAATGGGCA TCTTACAAAG CTAGCTACTC TTAATATACA TGACTATCTG 
ACCAAGGGCC ATGAGATGAC CAGCATTGGA CTGCTTCTTG GTGTTTCTGC TGCTAAGCTA 
GGCACAATGG ATATGTCAAT TACTCGACTT CTCAGCATCC ATATACCTGC TCTCTTACCT 
CCAACTTCAA CAGAACTTGA TGTCCCTCAC AATGTTCAGG TGGCTGCAGT GATAGGCATA 
GGCCTTGTCT ATCAAGGGAC AGCTCACAGG CATACTGCTG AAGTTCTGCT GGCTGAAATT 
GGACGTCCCC CAGGTCCAGA AATGGAATAT TGCACTGACA GGGAATCATA TTCTTTAGCA 
GCTGGTTTGG CACTGGGCAT GGTTTGCTTG GGGCATGGTA GCAACCTGAT AGGCATGTCT 
GATCTCAATG TACCTGAGCA GCTTTATCAG TATATGGTTG GTGGTCACAG GCGCTTCCAA 
GCAGGAATGC ATAGAGAAAA ACATAAATCT CCAAGTTATC AAATTAAAGA AGGTGACACA 
ATCAATGTAG ATGTGACTTG TCCTGGTGCA ACTTTAGCTT TGGCGATGAT CTACTTAAAA 
ACTAATAACA GATCTATTGC TGACTGGCTT CGAGCTCCTG ATACAATGTA TTTGTTGGAT 
TTTGTGAAAC CAGAATTTCT TCTGCTAAGG ACCCTTGCTC GATGCCTGAT TTTATGGGAT 
GACATTTTGC CTAATTCCAA GTGGGTTGAT AGTAATGTTC CACAAATCAT AAGGGAAAAT 
AGTATTTCTC TTAATGACAC TGAAATACCA TCCTCAGAGG ATCTGAACTT GGAAACTCTG 
GCGCAAGCAC ATGTCTATAT AATTGCTGGA GCTTGTTTGT CTCTGGGTTT CCGATTTGCT 
GGTTCAGAAA ACTTAGCAGC ATTTAATTGT TTGTATAAGT TTGCAAAAGA TTTTATGAAG 
TGTTTGTCTG CACCCACAGC TTCAGTGACA GGCCATTATA ACCTGGAAAC TTGCCTAAGT 
GTCGTGCTCC TCTCTCTTGC CATGGTGATG GCTGGCTCTG GAAATCTGAA GGTTCTCCAG 
CTTTGTCGCT TCATGCATAA GAAGACTGGT GGAGAAATGA ACTATGGTTT CCATTTGGCT 
CATCATATGG CTCTTGGACT TCTCTTTTTA GGAGGAGGCA GATATTCCTT GAGTACTTCA 
AATTCTTCAA TTGCTGCTCT TCTTTGTGCA CTTTATCCCC ACTTTCCAGT TCATAGCACT 
GACAATAGGT ATCATCTCCA GGCTCTTCGA CACTTGTATG TACTAGCAGC AGAACCAAGA 
CTTCTTGTTC CTGTTGATGT AGATACAAAT ACACCTTGCT ATGCATTATT AGAGGTTACA 
TACAAGGGTA CTCAGTGGTA TGAAGAAACA ACAGAAGAGC TAATGGCACC TACACTGCTT 
CCTGAGCTTC ACCTTTTAAA ACAGATTAAA GTGAAAGGCC CACGATACTG GGAATTGCTT 
ATAGATTTAA GTAAAGGAAT TCATCACTTG AAATCAATTC TTTCCAAGGA TGGAGTTTTA 
TATGTGAAAC TCAGGGCTGG ACAGCTCTCC TACAAAGAAG ATCCGATGGG CTGGCAAAGT 
TTGTTGGCTC AAACAGTCAC TCATAGAAAT TCTGAAGCCA GAGCTTTCAA GCCAGAAGCT 
ATTTCAGCTT TCACCTCTGA TCCAGCACTT TTGTCATTTG CTGAATATTT CTGCAAACCA 
ACTGTGAACT TGGGCCAGAA ACAGGAAATT CTTGATCTCT TTTCTTCAAT ACTTTATGAA 
TGTGTCACGC AGGAGAATCC AGAAATGCTG CCTGCATATA TAGCAATAGA TCAGGCTGTC 
AGAAGACTAG AAAGAAGGGA AATGTCTGAG ACTTTTGAAC TTTGGCAAAT AAAATTGGTA 
TTAGAATTCT TTAGCTCTAG AAGCCACCAG GAGAGAATGC AGAAGATCCC TAGTCGAGGA 
CTTTTTATGA ACTCTGAATT CCTTCCTGTG ATGAAATGCA CTATTGATAA TGCTTTGGAT 
CAATGGTTAC AAGCTGGAGG TGATGTGTGC CTGCATTCCT ATCTAAGTGG CCAGCCCACA 
GAAGAGTCTC ACCTAAGCAT GTTGGCGTGC TTCCTGATTT ATCATTCTGT TCCTACCCCA 
GGGCAGCTGT CCGCTGGAGG ATTAGAAGGA AGCATGAGTT TTTCTGAGCT ACTTCTAAAA 
TTCAAACAAC TGAAGATGCC AGTTCGAGCA TTGCTAAGAT TAGCTCCTTT GCTTCTTGGA 
AGTCCACAAT CAATGGTACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_031950993.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031806853.1 Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), transcript variant X3, mRNA.

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CloneID	OSa150761
Clone ID Related Accession (Same CDS sequence)	XM_023494825.1 , XM_031950993.1
Accession Version	XM_031950993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	anaphase-promoting complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACA TCTATGAAGA GAAGGTAACG ATGATTGCAG CAGGGGATTT GCAGGAGTTT 
GTTCCTTTTG GCCGTGATCA CTGCAAGCAC CACCCTAATG CGCTGAATCT TCAACTTCGG 
CAGCTGCAGC CTGCTTCTGA GCTGTGGTCT TCTGATGGAG CTGCTGGTTT GGTGGGATCC 
CTTCAGGAGG TTACAATCCA TGAAAAACAG AAGGAAAGCT GGCATTTAAG AAAAGGTATA 
AGTGATGTTG GAGAAGAAGT TGAATATGAT GAAGAATTGT ATGTGGCTGG TAATATGGTC 
ATCTGGAGCA AAGGAAGTAA AAGTCAGGCT TCTACTGTTT ATAAAGCTTT TACAGTGGAT 
AGTCCTGTTC TTCAGGCACT CTGGTGTGAC TTCATTATAT CACAGGATAA ATCTGAAAAA 
TATGATAATA GCAATGAAGT AGAAAAATGC ATATGCATAT TACAAAGTTC TTGCATAAAT 
GTGCATAGCA TGGAGGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTC 
TGGCCTACAA AATATGGTTT ATTATTTGAA AGAAGCAGTT CTGCACATGA AGTACCGCCA 
AGTCCACCCA GAGAACCTTT ACCCACTATG TTCAGTATGC TACATCCACT GGATGAAATA 
ACCCCTGTTG TTTGTAAATC TGGAGGTCTT TTTGGAACAT CAAGGGTACA GTATGTTGCT 
GATAGTGCAA TGAAAATTGT TTTTCTAAAT GCTGAGCCTT CTATCATAAT GACATATGAT 
GCTATTCAGG GTTTACATTC TCTCTGGATT CTGCGCAGAG TGAAACCTGA GGAACAGAAT 
GTGGTTTTAA AGTTCTCAGA GCAAGGAGGT ACCCCACAGC ATGTGGCTAC CAGCAGTTCC 
CTTACTGCCC ATCTCAGAAG TCTCTCAAAA GGAGATTCAC CTGTAACTTC ACCTTTTCAG 
AATTATTCTT CCATTCACAG TCAGTCTCGG TCAACATCCT CGCCCAGTTT ACATTCTCGT 
TCACCTTCTA TTTCTAACAT GGCAGCTCTT AGTCGTTCTC ATTCTCCTGC CTTAGGAGTA 
CACTCATTCT CAGGGGTGCA GAGATTCAAC TTTTCAAGCC ATAATCAATC TCCAAAGAGG 
CAGAGTGTTT CTCATTCTCC AAACAGTACT TCTAATGACT CCTTTCTTGC ACCAGAAGTG 
GAGCCAATTG TTCCAGAACT TTGTATTGAC CACTTATGGA CAGAAGCAAT TACCACTATG 
AGAGAGAAAA ATTCTCCAGC TTCAAAAGTA TTTATTACAT CTGACCTCTG TGGCCAGAAG 
TTTTTGTGTT TTTTAGTAGA ATCCCAACTA CAGCTTCGAT GTGTAAAGTT TCAAGAGAGT 
AATGATAAAA CCCAGTTGAT CTTTGGTTCT GTCACTAACT TACAGGCAAA GGATGCAGCA 
CCAGTAGAGA AAATTAATAC CATGCTGGTC CTAGAAGGTA ATGGAAACTT GGTACTATAC 
ACTGGATTGG TTCGGGTAGG GAAAGTTTTC ATTCCTGGAC TACCAGTTCC TTCTCTGACA 
ATGTCAAACA CAATGCCAAG ACCTAGTACT CCCTTGGACA GTGTTAGTAC TCCTAAGCCT 
CTAAGCAAAC CACTTGGCTC CTTGGATGAG GTAGTACTGC TATCTCCAGT TCCAGAACTG 
AGAGATTCTT CAAAAGTTCA TGATTCGCCT TATATTGATG ACTGTACTTT TCAGCAACTT 
GGAAGTTACA TTCACTCTGT TAGAGATCCT GTCCATAACA GAGTAACACT TGAACTCAGT 
AATGGTTCAA TGGTTCGGAT CACCATTCCT GAAATTGCCA CCTCAGAATT AGTAAAAAAG 
TGTTTACAAG CTGTCAAGTC TATACTGCCA AAAGAGGTAG CTGTGCAGAT GCTTGTCAAA 
TGGTACAATG TTCACAATGC TCCAGGAGGA CCCAGTTATC ATTCAGAATG GAATTTATTC 
GTAACTTGTC TAATGAATAT GATGGGCTAT AACACAGACC GCTTATCATG GATCCGTAAT 
CTTGACTTTG AAGGATCTCT TTCTCCAGTC ATAGCACCCA AAAAAGCAAG GCCTTCTGAC 
ACTGGATCAG ATGAGGACTG GGAATATTTA CTAAATTCAG AGTACCACCA GAATGTTGAG 
TCTCATCTTT TGACTAGAGC TTTGCATTTG AATCCTTTGG AAGTTTCACT GATGAAGGAG 
GATGTTTTAC ATAATCTTAC TTTGGATTCT TCTACACTTC TCTTCACTCA TATACCTGCT 
ATCTTTTTTG TGCTTCATCT TGTTTATGAA GAATTAAAAT TGAATAGTTT AATGGGAGAA 
GGAATTCGCT CACTTGTTGT TCTTCTAGTT CAGTTGGCAA GGGATTTACA GTTGGAGCCA 
TATTTAGATT ATTACTATAG AGATTATCCA ACACTTGTGA AAACTTCCCG ACAGTCATGC 
ATAATTGATC AAGCCCAAAT AGGACTTATG CATCACCCGT CATTTTTTAC ACCTGAGCCT 
CCAAGTATAT ATCAGTGGCT GAGTTCTTGC CTAAAGGGTG AAGGAGCATC ACCTTATCCT 
TACCTGTCTG GAGTCTGTGA ACGAAGCAAA CTCATAGTGT TGAGTGTTGC ACTCTATATA 
CTTGGTGATG AGAGAGCTGT TTCTAATGAA GCATCACAAT ATTTATGCAA AGTAAACTCA 
GCACCAAAAA AGAGTCAAGT GGACCAAAAT GACAATAGAT TTAGTTTCAG ACATAGTACA 
CCTGTTAATA GCCTCGCTGA AAAATTAGTA CTCTGGATGA CTAATGTAGG CTTTACCTTG 
AGAGATTTGG AGTCTCTTCC TTTTGGAGTT GCTCTTCCTA TCAGAGATGC TATTTATCAC 
TGCCGAGAAC AGCCTGCTTC AGATTGGCCA GAAGCTGTCT GCCTCTTGAT TGGGCGTCAG 
GATCTATCCA AGCAAGCCTG TGAAGGGAAC TTACCAAAAG GGAAATCTTC TGTGGCTCAG 
GTGCTCTCAT CAGATGTGCC TTCAGGAACT GAAGCAGAGG AAGAAGATGA TGGCATGAAT 
GATATGAATC AGGAAGTCAT GTCACTAATA TGGAGTGAAG ATTTAAGAGT CCAGGAGGTT 
CGAAGACTTC TCCAAAGTGC TCATCCTGTT CGAGTCAATG TAGTACAGAT GCCAGAAGTT 
AATGACCATG AATACATTGA AGAAAAAGAA AACAGGTTGC TCCAGTTGTG TCAGCGAACT 
ATGGCACTTC CCTTGGGGCG TGGAATGTTT ACTTTGTTTT CATACCATCC AGTTCCGACA 
GAACCGTTGC CTGTTCCTAA ACTGAATCTA ACTGGACGGG CTCCTCCTAG GAACACAACT 
GTGGACCTTA ACAGTGGAAA TATTGATGTG CCTCCCAACA TGGCATGTTG GGCTAGTTTT 
CACAATGGTG TTGCTGCGGG GTTAAAAATA GCACCTGCTT CCCAGATTGA CTCAGCTTGG 
ATTGTCTACA ATAAACCAAA GAATGCAGAA TTGGCCAATG AATATGCAGG TTTTCTTATG 
GCCTTAGGCT TGAATGGGCA TCTTACAAAG CTAGCTACTC TTAATATACA TGACTATCTG 
ACCAAGGGCC ATGAGATGAC CAGCATTGGA CTGCTTCTTG GTGTTTCTGC TGCTAAGCTA 
GGCACAATGG ATATGTCAAT TACTCGACTT CTCAGCATCC ATATACCTGC TCTCTTACCT 
CCAACTTCAA CAGAACTTGA TGTCCCTCAC AATGTTCAGG TGGCTGCAGT GATAGGCATA 
GGCCTTGTCT ATCAAGGGAC AGCTCACAGG CATACTGCTG AAGTTCTGCT GGCTGAAATT 
GGACGTCCCC CAGGTCCAGA AATGGAATAT TGCACTGACA GGGAATCATA TTCTTTAGCA 
GCTGGTTTGG CACTGGGCAT GGTTTGCTTG GGGCATGGTA GCAACCTGAT AGGCATGTCT 
GATCTCAATG TACCTGAGCA GCTTTATCAG TATATGGTTG GTGGTCACAG GCGCTTCCAA 
GCAGGAATGC ATAGAGAAAA ACATAAATCT CCAAGTTATC AAATTAAAGA AGGTGACACA 
ATCAATGTAG ATGTGACTTG TCCTGGTGCA ACTTTAGCTT TGGCGATGAT CTACTTAAAA 
ACTAATAACA GATCTATTGC TGACTGGCTT CGAGCTCCTG ATACAATGTA TTTGTTGGAT 
TTTGTGAAAC CAGAATTTCT TCTGCTAAGG ACCCTTGCTC GATGCCTGAT TTTATGGGAT 
GACATTTTGC CTAATTCCAA GTGGGTTGAT AGTAATGTTC CACAAATCAT AAGGGAAAAT 
AGTATTTCTC TTAATGACAC TGAAATACCA TCCTCAGAGG ATCTGAACTT GGAAACTCTG 
GCGCAAGCAC ATGTCTATAT AATTGCTGGA GCTTGTTTGT CTCTGGGTTT CCGATTTGCT 
GGTTCAGAAA ACTTAGCAGC ATTTAATTGT TTGTATAAGT TTGCAAAAGA TTTTATGAAG 
TGTTTGTCTG CACCCACAGC TTCAGTGACA GGCCATTATA ACCTGGAAAC TTGCCTAAGT 
GTCGTGCTCC TCTCTCTTGC CATGGTGATG GCTGGCTCTG GAAATCTGAA GGTTCTCCAG 
CTTTGTCGCT TCATGCATAA GAAGACTGGT GGAGAAATGA ACTATGGTTT CCATTTGGCT 
CATCATATGG CTCTTGGACT TCTCTTTTTA GGAGGAGGCA GATATTCCTT GAGTACTTCA 
AATTCTTCAA TTGCTGCTCT TCTTTGTGCA CTTTATCCCC ACTTTCCAGT TCATAGCACT 
GACAATAGGT ATCATCTCCA GGCTCTTCGA CACTTGTATG TACTAGCAGC AGAACCAAGA 
CTTCTTGTTC CTGTTGATGT AGATACAAAT ACACCTTGCT ATGCATTATT AGAGGTTACA 
TACAAGGGTA CTCAGTGGTA TGAAGAAACA ACAGAAGAGC TAATGGCACC TACACTGCTT 
CCTGAGCTTC ACCTTTTAAA ACAGATTAAA GTGAAAGGCC CACGATACTG GGAATTGCTT 
ATAGATTTAA GTAAAGGAAT TCATCACTTG AAATCAATTC TTTCCAAGGA TGGAGTTTTA 
TATGTGAAAC TCAGGGCTGG ACAGCTCTCC TACAAAGAAG ATCCGATGGG CTGGCAAAGT 
TTGTTGGCTC AAACAGTCAC TCATAGAAAT TCTGAAGCCA GAGCTTTCAA GCCAGAAGCT 
ATTTCAGCTT TCACCTCTGA TCCAGCACTT TTGTCATTTG CTGAATATTT CTGCAAACCA 
ACTGTGAACT TGGGCCAGAA ACAGGAAATT CTTGATCTCT TTTCTTCAAT ACTTTATGAA 
TGTGTCACGC AGGAGAATCC AGAAATGCTG CCTGCATATA TAGCAATAGA TCAGGCTGTC 
AGAAGACTAG AAAGAAGGGA AATGTCTGAG ACTTTTGAAC TTTGGCAAAT AAAATTGGTA 
TTAGAATTCT TTAGCTCTAG AAGCCACCAG GAGAGAATGC AGAAGATCCC TAGTCGAGGA 
CTTTTTATGA ACTCTGAATT CCTTCCTGTG ATGAAATGCA CTATTGATAA TGCTTTGGAT 
CAATGGTTAC AAGCTGGAGG TGATGTGTGC CTGCATTCCT ATCTAAGTGG CCAGCCCACA 
GAAGAGTCTC ACCTAAGCAT GTTGGCGTGC TTCCTGATTT ATCATTCTGT TCCTACCCCA 
GGGCAGCTGT CCGCTGGAGG ATTAGAAGGA AGCATGAGTT TTTCTGAGCT ACTTCTAAAA 
TTCAAACAAC TGAAGATGCC AGTTCGAGCA TTGCTAAGAT TAGCTCCTTT GCTTCTTGGA 
AGTCCACAAT CAATGGTACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031806853.1
CDS	361..6204
Translation	MSNIYEEKVTMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWHLRKGISDVGEEVEYDEELYVAGNMVIWSKGSKSQASTVYKAFTVDSPVLQALWCDFIISQDKSEKYDNSNEVEKCICILQSSCINVHSMEGKDYIASLPFQVANVWPTKYGLLFERSSSAHEVPPSPPREPLPTMFSMLHPLDEITPVVCKSGGLFGTSRVQYVADSAMKIVFLNAEPSIIMTYDAIQGLHSLWILRRVKPEEQNVVLKFSEQGGTPQHVATSSSLTAHLRSLSKGDSPVTSPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRSHSPALGVHSFSGVQRFNFSSHNQSPKRQSVSHSPNSTSNDSFLAPEVEPIVPELCIDHLWTEAITTMREKNSPASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSVTNLQAKDAAPVEKINTMLVLEGNGNLVLYTGLVRVGKVFIPGLPVPSLTMSNTMPRPSTPLDSVSTPKPLSKPLGSLDEVVLLSPVPELRDSSKVHDSPYIDDCTFQQLGSYIHSVRDPVHNRVTLELSNGSMVRITIPEIATSELVKKCLQAVKSILPKEVAVQMLVKWYNVHNAPGGPSYHSEWNLFVTCLMNMMGYNTDRLSWIRNLDFEGSLSPVIAPKKARPSDTGSDEDWEYLLNSEYHQNVESHLLTRALHLNPLEVSLMKEDVLHNLTLDSSTLLFTHIPAIFFVLHLVYEELKLNSLMGEGIRSLVVLLVQLARDLQLEPYLDYYYRDYPTLVKTSRQSCIIDQAQIGLMHHPSFFTPEPPSIYQWLSSCLKGEGASPYPYLSGVCERSKLIVLSVALYILGDERAVSNEASQYLCKVNSAPKKSQVDQNDNRFSFRHSTPVNSLAEKLVLWMTNVGFTLRDLESLPFGVALPIRDAIYHCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSSVAQVLSSDVPSGTEAEEEDDGMNDMNQEVMSLIWSEDLRVQEVRRLLQSAHPVRVNVVQMPEVNDHEYIEEKENRLLQLCQRTMALPLGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMACWASFHNGVAAGLKIAPASQIDSAWIVYNKPKNAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHNVQVAAVIGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRFQAGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLNDTEIPSSEDLNLETLAQAHVYIIAGACLSLGFRFAGSENLAAFNCLYKFAKDFMKCLSAPTASVTGHYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFMHKKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPVHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALLEVTYKGTQWYEETTEELMAPTLLPELHLLKQIKVKGPRYWELLIDLSKGIHHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVTHRNSEARAFKPEAISAFTSDPALLSFAEYFCKPTVNLGQKQEILDLFSSILYECVTQENPEMLPAYIAIDQAVRRLERREMSETFELWQIKLVLEFFSSRSHQERMQKIPSRGLFMNSEFLPVMKCTIDNALDQWLQAGGDVCLHSYLSGQPTEESHLSMLACFLIYHSVPTPGQLSAGGLEGSMSFSELLLKFKQLKMPVRALLRLAPLLLGSPQSMVL

Target ORF information:

RefSeq Version	XM_031950993.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031950993.1

ORF Insert Sequence:

ATGTCGAACA TCTATGAAGA GAAGGTAACG ATGATTGCAG CAGGGGATTT GCAGGAGTTT 
GTTCCTTTTG GCCGTGATCA CTGCAAGCAC CACCCTAATG CGCTGAATCT TCAACTTCGG 
CAGCTGCAGC CTGCTTCTGA GCTGTGGTCT TCTGATGGAG CTGCTGGTTT GGTGGGATCC 
CTTCAGGAGG TTACAATCCA TGAAAAACAG AAGGAAAGCT GGCATTTAAG AAAAGGTATA 
AGTGATGTTG GAGAAGAAGT TGAATATGAT GAAGAATTGT ATGTGGCTGG TAATATGGTC 
ATCTGGAGCA AAGGAAGTAA AAGTCAGGCT TCTACTGTTT ATAAAGCTTT TACAGTGGAT 
AGTCCTGTTC TTCAGGCACT CTGGTGTGAC TTCATTATAT CACAGGATAA ATCTGAAAAA 
TATGATAATA GCAATGAAGT AGAAAAATGC ATATGCATAT TACAAAGTTC TTGCATAAAT 
GTGCATAGCA TGGAGGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTC 
TGGCCTACAA AATATGGTTT ATTATTTGAA AGAAGCAGTT CTGCACATGA AGTACCGCCA 
AGTCCACCCA GAGAACCTTT ACCCACTATG TTCAGTATGC TACATCCACT GGATGAAATA 
ACCCCTGTTG TTTGTAAATC TGGAGGTCTT TTTGGAACAT CAAGGGTACA GTATGTTGCT 
GATAGTGCAA TGAAAATTGT TTTTCTAAAT GCTGAGCCTT CTATCATAAT GACATATGAT 
GCTATTCAGG GTTTACATTC TCTCTGGATT CTGCGCAGAG TGAAACCTGA GGAACAGAAT 
GTGGTTTTAA AGTTCTCAGA GCAAGGAGGT ACCCCACAGC ATGTGGCTAC CAGCAGTTCC 
CTTACTGCCC ATCTCAGAAG TCTCTCAAAA GGAGATTCAC CTGTAACTTC ACCTTTTCAG 
AATTATTCTT CCATTCACAG TCAGTCTCGG TCAACATCCT CGCCCAGTTT ACATTCTCGT 
TCACCTTCTA TTTCTAACAT GGCAGCTCTT AGTCGTTCTC ATTCTCCTGC CTTAGGAGTA 
CACTCATTCT CAGGGGTGCA GAGATTCAAC TTTTCAAGCC ATAATCAATC TCCAAAGAGG 
CAGAGTGTTT CTCATTCTCC AAACAGTACT TCTAATGACT CCTTTCTTGC ACCAGAAGTG 
GAGCCAATTG TTCCAGAACT TTGTATTGAC CACTTATGGA CAGAAGCAAT TACCACTATG 
AGAGAGAAAA ATTCTCCAGC TTCAAAAGTA TTTATTACAT CTGACCTCTG TGGCCAGAAG 
TTTTTGTGTT TTTTAGTAGA ATCCCAACTA CAGCTTCGAT GTGTAAAGTT TCAAGAGAGT 
AATGATAAAA CCCAGTTGAT CTTTGGTTCT GTCACTAACT TACAGGCAAA GGATGCAGCA 
CCAGTAGAGA AAATTAATAC CATGCTGGTC CTAGAAGGTA ATGGAAACTT GGTACTATAC 
ACTGGATTGG TTCGGGTAGG GAAAGTTTTC ATTCCTGGAC TACCAGTTCC TTCTCTGACA 
ATGTCAAACA CAATGCCAAG ACCTAGTACT CCCTTGGACA GTGTTAGTAC TCCTAAGCCT 
CTAAGCAAAC CACTTGGCTC CTTGGATGAG GTAGTACTGC TATCTCCAGT TCCAGAACTG 
AGAGATTCTT CAAAAGTTCA TGATTCGCCT TATATTGATG ACTGTACTTT TCAGCAACTT 
GGAAGTTACA TTCACTCTGT TAGAGATCCT GTCCATAACA GAGTAACACT TGAACTCAGT 
AATGGTTCAA TGGTTCGGAT CACCATTCCT GAAATTGCCA CCTCAGAATT AGTAAAAAAG 
TGTTTACAAG CTGTCAAGTC TATACTGCCA AAAGAGGTAG CTGTGCAGAT GCTTGTCAAA 
TGGTACAATG TTCACAATGC TCCAGGAGGA CCCAGTTATC ATTCAGAATG GAATTTATTC 
GTAACTTGTC TAATGAATAT GATGGGCTAT AACACAGACC GCTTATCATG GATCCGTAAT 
CTTGACTTTG AAGGATCTCT TTCTCCAGTC ATAGCACCCA AAAAAGCAAG GCCTTCTGAC 
ACTGGATCAG ATGAGGACTG GGAATATTTA CTAAATTCAG AGTACCACCA GAATGTTGAG 
TCTCATCTTT TGACTAGAGC TTTGCATTTG AATCCTTTGG AAGTTTCACT GATGAAGGAG 
GATGTTTTAC ATAATCTTAC TTTGGATTCT TCTACACTTC TCTTCACTCA TATACCTGCT 
ATCTTTTTTG TGCTTCATCT TGTTTATGAA GAATTAAAAT TGAATAGTTT AATGGGAGAA 
GGAATTCGCT CACTTGTTGT TCTTCTAGTT CAGTTGGCAA GGGATTTACA GTTGGAGCCA 
TATTTAGATT ATTACTATAG AGATTATCCA ACACTTGTGA AAACTTCCCG ACAGTCATGC 
ATAATTGATC AAGCCCAAAT AGGACTTATG CATCACCCGT CATTTTTTAC ACCTGAGCCT 
CCAAGTATAT ATCAGTGGCT GAGTTCTTGC CTAAAGGGTG AAGGAGCATC ACCTTATCCT 
TACCTGTCTG GAGTCTGTGA ACGAAGCAAA CTCATAGTGT TGAGTGTTGC ACTCTATATA 
CTTGGTGATG AGAGAGCTGT TTCTAATGAA GCATCACAAT ATTTATGCAA AGTAAACTCA 
GCACCAAAAA AGAGTCAAGT GGACCAAAAT GACAATAGAT TTAGTTTCAG ACATAGTACA 
CCTGTTAATA GCCTCGCTGA AAAATTAGTA CTCTGGATGA CTAATGTAGG CTTTACCTTG 
AGAGATTTGG AGTCTCTTCC TTTTGGAGTT GCTCTTCCTA TCAGAGATGC TATTTATCAC 
TGCCGAGAAC AGCCTGCTTC AGATTGGCCA GAAGCTGTCT GCCTCTTGAT TGGGCGTCAG 
GATCTATCCA AGCAAGCCTG TGAAGGGAAC TTACCAAAAG GGAAATCTTC TGTGGCTCAG 
GTGCTCTCAT CAGATGTGCC TTCAGGAACT GAAGCAGAGG AAGAAGATGA TGGCATGAAT 
GATATGAATC AGGAAGTCAT GTCACTAATA TGGAGTGAAG ATTTAAGAGT CCAGGAGGTT 
CGAAGACTTC TCCAAAGTGC TCATCCTGTT CGAGTCAATG TAGTACAGAT GCCAGAAGTT 
AATGACCATG AATACATTGA AGAAAAAGAA AACAGGTTGC TCCAGTTGTG TCAGCGAACT 
ATGGCACTTC CCTTGGGGCG TGGAATGTTT ACTTTGTTTT CATACCATCC AGTTCCGACA 
GAACCGTTGC CTGTTCCTAA ACTGAATCTA ACTGGACGGG CTCCTCCTAG GAACACAACT 
GTGGACCTTA ACAGTGGAAA TATTGATGTG CCTCCCAACA TGGCATGTTG GGCTAGTTTT 
CACAATGGTG TTGCTGCGGG GTTAAAAATA GCACCTGCTT CCCAGATTGA CTCAGCTTGG 
ATTGTCTACA ATAAACCAAA GAATGCAGAA TTGGCCAATG AATATGCAGG TTTTCTTATG 
GCCTTAGGCT TGAATGGGCA TCTTACAAAG CTAGCTACTC TTAATATACA TGACTATCTG 
ACCAAGGGCC ATGAGATGAC CAGCATTGGA CTGCTTCTTG GTGTTTCTGC TGCTAAGCTA 
GGCACAATGG ATATGTCAAT TACTCGACTT CTCAGCATCC ATATACCTGC TCTCTTACCT 
CCAACTTCAA CAGAACTTGA TGTCCCTCAC AATGTTCAGG TGGCTGCAGT GATAGGCATA 
GGCCTTGTCT ATCAAGGGAC AGCTCACAGG CATACTGCTG AAGTTCTGCT GGCTGAAATT 
GGACGTCCCC CAGGTCCAGA AATGGAATAT TGCACTGACA GGGAATCATA TTCTTTAGCA 
GCTGGTTTGG CACTGGGCAT GGTTTGCTTG GGGCATGGTA GCAACCTGAT AGGCATGTCT 
GATCTCAATG TACCTGAGCA GCTTTATCAG TATATGGTTG GTGGTCACAG GCGCTTCCAA 
GCAGGAATGC ATAGAGAAAA ACATAAATCT CCAAGTTATC AAATTAAAGA AGGTGACACA 
ATCAATGTAG ATGTGACTTG TCCTGGTGCA ACTTTAGCTT TGGCGATGAT CTACTTAAAA 
ACTAATAACA GATCTATTGC TGACTGGCTT CGAGCTCCTG ATACAATGTA TTTGTTGGAT 
TTTGTGAAAC CAGAATTTCT TCTGCTAAGG ACCCTTGCTC GATGCCTGAT TTTATGGGAT 
GACATTTTGC CTAATTCCAA GTGGGTTGAT AGTAATGTTC CACAAATCAT AAGGGAAAAT 
AGTATTTCTC TTAATGACAC TGAAATACCA TCCTCAGAGG ATCTGAACTT GGAAACTCTG 
GCGCAAGCAC ATGTCTATAT AATTGCTGGA GCTTGTTTGT CTCTGGGTTT CCGATTTGCT 
GGTTCAGAAA ACTTAGCAGC ATTTAATTGT TTGTATAAGT TTGCAAAAGA TTTTATGAAG 
TGTTTGTCTG CACCCACAGC TTCAGTGACA GGCCATTATA ACCTGGAAAC TTGCCTAAGT 
GTCGTGCTCC TCTCTCTTGC CATGGTGATG GCTGGCTCTG GAAATCTGAA GGTTCTCCAG 
CTTTGTCGCT TCATGCATAA GAAGACTGGT GGAGAAATGA ACTATGGTTT CCATTTGGCT 
CATCATATGG CTCTTGGACT TCTCTTTTTA GGAGGAGGCA GATATTCCTT GAGTACTTCA 
AATTCTTCAA TTGCTGCTCT TCTTTGTGCA CTTTATCCCC ACTTTCCAGT TCATAGCACT 
GACAATAGGT ATCATCTCCA GGCTCTTCGA CACTTGTATG TACTAGCAGC AGAACCAAGA 
CTTCTTGTTC CTGTTGATGT AGATACAAAT ACACCTTGCT ATGCATTATT AGAGGTTACA 
TACAAGGGTA CTCAGTGGTA TGAAGAAACA ACAGAAGAGC TAATGGCACC TACACTGCTT 
CCTGAGCTTC ACCTTTTAAA ACAGATTAAA GTGAAAGGCC CACGATACTG GGAATTGCTT 
ATAGATTTAA GTAAAGGAAT TCATCACTTG AAATCAATTC TTTCCAAGGA TGGAGTTTTA 
TATGTGAAAC TCAGGGCTGG ACAGCTCTCC TACAAAGAAG ATCCGATGGG CTGGCAAAGT 
TTGTTGGCTC AAACAGTCAC TCATAGAAAT TCTGAAGCCA GAGCTTTCAA GCCAGAAGCT 
ATTTCAGCTT TCACCTCTGA TCCAGCACTT TTGTCATTTG CTGAATATTT CTGCAAACCA 
ACTGTGAACT TGGGCCAGAA ACAGGAAATT CTTGATCTCT TTTCTTCAAT ACTTTATGAA 
TGTGTCACGC AGGAGAATCC AGAAATGCTG CCTGCATATA TAGCAATAGA TCAGGCTGTC 
AGAAGACTAG AAAGAAGGGA AATGTCTGAG ACTTTTGAAC TTTGGCAAAT AAAATTGGTA 
TTAGAATTCT TTAGCTCTAG AAGCCACCAG GAGAGAATGC AGAAGATCCC TAGTCGAGGA 
CTTTTTATGA ACTCTGAATT CCTTCCTGTG ATGAAATGCA CTATTGATAA TGCTTTGGAT 
CAATGGTTAC AAGCTGGAGG TGATGTGTGC CTGCATTCCT ATCTAAGTGG CCAGCCCACA 
GAAGAGTCTC ACCTAAGCAT GTTGGCGTGC TTCCTGATTT ATCATTCTGT TCCTACCCCA 
GGGCAGCTGT CCGCTGGAGG ATTAGAAGGA AGCATGAGTT TTTCTGAGCT ACTTCTAAAA 
TTCAAACAAC TGAAGATGCC AGTTCGAGCA TTGCTAAGAT TAGCTCCTTT GCTTCTTGGA 
AGTCCACAAT CAATGGTACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_031950991.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031806851.1 Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa224312
Clone ID Related Accession (Same CDS sequence)	XM_031950991.1 , XM_031950992.1
Accession Version	XM_031950991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	anaphase-promoting complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACA TCTATGAAGA GAAGGTAACG ATGATTGCAG CAGGGGATTT GCAGGAGTTT 
GTTCCTTTTG GCCGTGATCA CTGCAAGCAC CACCCTAATG CGCTGAATCT TCAACTTCGG 
CAGCTGCAGC CTGCTTCTGA GCTGTGGTCT TCTGATGGAG CTGCTGGTTT GGTGGGATCC 
CTTCAGGAGG TTACAATCCA TGAAAAACAG AAGGAAAGCT GGCATTTAAG AAAAGGTATA 
AGTGATGTTG GAGAAGAAGT TGAATATGAT GAAGAATTGT ATGTGGCTGG TAATATGGTC 
ATCTGGAGCA AAGGAAGTAA AAGTCAGGCT TCTACTGTTT ATAAAGCTTT TACAGTGGAT 
AGTCCTGTTC TTCAGGCACT CTGGTGTGAC TTCATTATAT CACAGGATAA ATCTGAAAAA 
TATGATAATA GCAATGAAGT AGAAAAATGC ATATGCATAT TACAAAGTTC TTGCATAAAT 
GTGCATAGCA TGGAGGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTC 
TGGCCTACAA AATATGGTTT ATTATTTGAA AGAAGCAGTT CTGCACATGA AGTACCGCCA 
AGTCCACCCA GAGAACCTTT ACCCACTATG TTCAGTATGC TACATCCACT GGATGAAATA 
ACCCCTGTTG TTTGTAAATC TGGAGGTCTT TTTGGAACAT CAAGGGTACA GTATGTTGCT 
GATAGTGCAA TGAAAATTGT TTTTCTAAAT GCTGAGCCTT CTATCATAAT GACATATGAT 
GCTATTCAGG GTTTACATTC TCTCTGGATT CTGCGCAGAG TGAAACCTGA GGAACAGAAT 
GTGGTTTTAA AGTTCTCAGA GCAAGGAGGT ACCCCACAGC ATGTGGCTAC CAGCAGTTCC 
CTTACTGCCC ATCTCAGAAG TCTCTCAAAA GGAGATTCAC CTGTAACTTC ACCTTTTCAG 
AATTATTCTT CCATTCACAG TCAGTCTCGG TCAACATCCT CGCCCAGTTT ACATTCTCGT 
TCACCTTCTA TTTCTAACAT GGCAGCTCTT AGTCGTTCTC ATTCTCCTGC CTTAGGAGTA 
CACTCATTCT CAGGGGTGCA GAGATTCAAC TTTTCAAGCC ATAATCAATC TCCAAAGAGG 
CAGAGTGTTT CTCATTCTCC AAACAGTACT TCTAATGACT CCTTTCTTGC ACCAGAAGTG 
GAGCCAATTG TTCCAGAACT TTGTATTGAC CACTTATGGA CAGAAGCAAT TACCACTATG 
AGAGAGAAAA ATTCTCCAGC TTCAAAAGTA TTTATTACAT CTGACCTCTG TGGCCAGAAG 
TTTTTGTGTT TTTTAGTAGA ATCCCAACTA CAGCTTCGAT GTGTAAAGTT TCAAGAGAGT 
AATGATAAAA CCCAGTTGAT CTTTGGTTCT GTCACTAACT TACAGGCAAA GGATGCAGCA 
CCAGTAGAGA AAATTAATAC CATGCTGGTC CTAGAAGGTA ATGGAAACTT GGTACTATAC 
ACTGGATTGG TTCGGGTAGG GAAAGTTTTC ATTCCTGGAC TACCAGTTCC TTCTCTGACA 
ATGTCAAACA CAATGCCAAG ACCTAGTACT CCCTTGGACA GTGTTAGTAC TCCTAAGCCT 
CTAAGCAAAC CACTTGGCTC CTTGGATGAG GTAGTACTGC TATCTCCAGT TCCAGAACTG 
AGAGATTCTT CAAAAGTTCA TGATTCGCCT TATATTGATG ACTGTACTTT TCAGCAACTT 
GGAAGTTACA TTCACTCTGT TAGAGATCCT GTCCATAACA GAGTAACACT TGAACTCAGT 
AATGGTTCAA TGGTTCGGAT CACCATTCCT GAAATTGCCA CCTCAGAATT AGTAAAAAAG 
TGTTTACAAG CTGTCAAGTC TATACTGCCA AAAGAGGTAG CTGTGCAGAT GCTTGTCAAA 
TGGTACAATG TTCACAATGC TCCAGGAGGA CCCAGTTATC ATTCAGAATG GAATTTATTC 
GTAACTTGTC TAATGAATAT GATGGGCTAT AACACAGACC GCTTATCATG GATCCGTAAT 
CTTGACTTTG AAGGATCTCT TTCTCCAGTC ATAGCACCCA AAAAAGCAAG GCCTTCTGAC 
ACTGGATCAG ATGAGGACTG GGAATATTTA CTAAATTCAG AGTACCACCA GAATGTTGAG 
TCTCATCTTT TGACTAGAGC TTTGCATTTG AATCCTTTGG AAGTTTCACT GATGAAGGAG 
GATGTTTTAC ATAATCTTAC TTTGGATTCT TCTACACTTC TCTTCACTCA TATACCTGCT 
ATCTTTTTTG TGCTTCATCT TGTTTATGAA GAATTAAAAT TGAATAGTTT AATGGGAGAA 
GGAATTCGCT CACTTGTTGT TCTTCTAGTT CAGTTGGCAA GGGATTTACA GTTGGAGCCA 
TATTTAGATT ATTACTATAG AGATTATCCA ACACTTGTGA AAACTTCCCG ACAGTCATGC 
ATAATTGATC AAGCCCAAAT AGGACTTATG CATCACCCGT CATTTTTTAC ACCTGAGCCT 
CCAAGTATAT ATCAGTGGCT GAGTTCTTGC CTAAAGGGTG AAGGAGCATC ACCTTATCCT 
TACCTGTCTG GAGTCTGTGA ACGAAGCAAA CTCATAGTGT TGAGTGTTGC ACTCTATATA 
CTTGGTGATG AGAGAGCTGT TTCTAATGAA GCATCACAAT ATTTATGCAA AGTAAACTCA 
GCACCAAAAA AGAGTCAAGT GGACCAAAAT GACAATAGAT TTAGTTTCAG ACATAGTACA 
CCTGTTAATA GCCTCGCTGA AAAATTAGTA CTCTGGATGA CTAATGTAGG CTTTACCTTG 
AGAGATTTGG AGTCTCTTCC TTTTGGAGTT GCTCTTCCTA TCAGAGATGC TATTTATCAC 
TGCCGAGAAC AGCCTGCTTC AGATTGGCCA GAAGCTGTCT GCCTCTTGAT TGGGCGTCAG 
GATCTATCCA AGCAAGCCTG TGAAGGGAAC TTACCAAAAG GGAAATCTTC TGTGGCTCAG 
GTGCTCTCAT CAGATGTGCC TTCAGGAACT GAAGCAGAGG AAGAAGATGA TGGCATGAAT 
GATATGAATC AGGAAGTCAT GTCACTAATA TGGAGTGAAG ATTTAAGAGT CCAGGAGGTT 
CGAAGACTTC TCCAAAGTGC TCATCCTGTT CGAGTCAATG TAGTACAGAT GCCAGAAGTT 
AATGACCATG AATACATTGA AGAAAAAGAA AACAGGTTGC TCCAGTTGTG TCAGCGAACT 
ATGGCACTTC CCTTGGGGCG TGGAATGTTT ACTTTGTTTT CATACCATCC AGTTCCGACA 
GAACCGTTGC CTGTTCCTAA ACTGAATCTA ACTGGACGGG CTCCTCCTAG GAACACAACT 
GTGGACCTTA ACAGTGGAAA TATTGATGTG CCTCCCAACA TGGCATGTTG GGCTAGTTTT 
CACAATGGTG TTGCTGCGGG GTTAAAAATA GCACCTGCTT CCCAGATTGA CTCAGCTTGG 
ATTGTCTACA ATAAACCAAA GAATGCAGAA TTGGCCAATG AATATGCAGG TTTTCTTATG 
GCCTTAGGCT TGAATGGGCA TCTTACAAAG CTAGCTACTC TTAATATACA TGACTATCTG 
ACCAAGGGCC ATGAGATGAC CAGCATTGGA CTGCTTCTTG GTGTTTCTGC TGCTAAGCTA 
GGCACAATGG ATATGTCAAT TACTCGACTT CTCAGCATCC ATATACCTGC TCTCTTACCT 
CCAACTTCAA CAGAACTTGA TGTCCCTCAC AATGTTCAGG TGGCTGCAGT GATAGGCATA 
GGCCTTGTCT ATCAAGGGAC AGCTCACAGG CATACTGCTG AAGTTCTGCT GGCTGAAATT 
GGACGTCCCC CAGGTCCAGA AATGGAATAT TGCACTGACA GGGAATCATA TTCTTTAGCA 
GCTGGTTTGG CACTGGGCAT GGTTTGCTTG GGGCATGGTA GCAACCTGAT AGGCATGTCT 
GATCTCAATG TACCTGAGCA GCTTTATCAG TATATGGTTG GTGGTCACAG GCGCTTCCAA 
GCAGGAATGC ATAGAGAAAA ACATAAATCT CCAAGTTATC AAATTAAAGA AGGTGACACA 
ATCAATGTAG ATGTGACTTG TCCTGGTGCA ACTTTAGCTT TGGCGATGAT CTACTTAAAA 
ACTAATAACA GATCTATTGC TGACTGGCTT CGAGCTCCTG ATACAATGTA TTTGTTGGAT 
TTTGTGAAAC CAGAATTTCT TCTGCTAAGG ACCCTTGCTC GATGCCTGAT TTTATGGGAT 
GACATTTTGC CTAATTCCAA GTGGGTTGAT AGTAATGTTC CACAAATCAT AAGGGAAAAT 
AGTATTTCTC TTAATGACAC TGAAATACCA TCCTCAGAGG ATCTGAACTT GGAAACTCTG 
GCGCAAGCAC ATGTCTATAT AATTGCTGGA GCTTGTTTGT CTCTGGGTTT CCGATTTGCT 
GGTTCAGAAA ACTTAGCAGC ATTTAATTGT TTGTATAAGT TTGCAAAAGA TTTTATGAAG 
TGTTTGTCTG CACCCACAGC TTCAGTGACA GGCCATTATA ACCTGGAAAC TTGCCTAAGT 
GTCGTGCTCC TCTCTCTTGC CATGGTGATG GCTGGCTCTG GAAATCTGAA GGTTCTCCAG 
CTTTGTCGCT TCATGCATAA GAAGACTGGT GGAGAAATGA ACTATGGTTT CCATTTGGCT 
CATCATATGG CTCTTGGACT TCTCTTTTTA GGAGGAGGCA GATATTCCTT GAGTACTTCA 
AATTCTTCAA TTGCTGCTCT TCTTTGTGCA CTTTATCCCC ACTTTCCAGT TCATAGCACT 
GACAATAGGT ATCATCTCCA GGCTCTTCGA CACTTGTATG TACTAGCAGC AGAACCAAGA 
CTTCTTGTTC CTGTTGATGT AGATACAAAT ACACCTTGCT ATGCATTATT AGAGGTTACA 
TACAAGCACA CTGTACTGAG CTCTTCTTCC TTTGATGATA CAAAGGGTAC TCAGTGGTAT 
GAAGAAACAA CAGAAGAGCT AATGGCACCT ACACTGCTTC CTGAGCTTCA CCTTTTAAAA 
CAGATTAAAG TGAAAGGCCC ACGATACTGG GAATTGCTTA TAGATTTAAG TAAAGGAATT 
CATCACTTGA AATCAATTCT TTCCAAGGAT GGAGTTTTAT ATGTGAAACT CAGGGCTGGA 
CAGCTCTCCT ACAAAGAAGA TCCGATGGGC TGGCAAAGTT TGTTGGCTCA AACAGTCACT 
CATAGAAATT CTGAAGCCAG AGCTTTCAAG CCAGAAGCTA TTTCAGCTTT CACCTCTGAT 
CCAGCACTTT TGTCATTTGC TGAATATTTC TGCAAACCAA CTGTGAACTT GGGCCAGAAA 
CAGGAAATTC TTGATCTCTT TTCTTCAATA CTTTATGAAT GTGTCACGCA GGAGAATCCA 
GAAATGCTGC CTGCATATAT AGCAATAGAT CAGGCTGTCA GAAGACTAGA AAGAAGGGAA 
ATGTCTGAGA CTTTTGAACT TTGGCAAATA AAATTGGTAT TAGAATTCTT TAGCTCTAGA 
AGCCACCAGG AGAGAATGCA GAAGATCCCT AGTCGAGGAC TTTTTATGAA CTCTGAATTC 
CTTCCTGTGA TGAAATGCAC TATTGATAAT GCTTTGGATC AATGGTTACA AGCTGGAGGT 
GATGTGTGCC TGCATTCCTA TCTAAGTGGC CAGCCCACAG AAGAGTCTCA CCTAAGCATG 
TTGGCGTGCT TCCTGATTTA TCATTCTGTT CCTACCCCAG GGCAGCTGTC CGCTGGAGGA 
TTAGAAGGAA GCATGAGTTT TTCTGAGCTA CTTCTAAAAT TCAAACAACT GAAGATGCCA 
GTTCGAGCAT TGCTAAGATT AGCTCCTTTG CTTCTTGGAA GTCCACAATC AATGGTACTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031806851.1
CDS	97..5979
Translation	MSNIYEEKVTMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWHLRKGISDVGEEVEYDEELYVAGNMVIWSKGSKSQASTVYKAFTVDSPVLQALWCDFIISQDKSEKYDNSNEVEKCICILQSSCINVHSMEGKDYIASLPFQVANVWPTKYGLLFERSSSAHEVPPSPPREPLPTMFSMLHPLDEITPVVCKSGGLFGTSRVQYVADSAMKIVFLNAEPSIIMTYDAIQGLHSLWILRRVKPEEQNVVLKFSEQGGTPQHVATSSSLTAHLRSLSKGDSPVTSPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRSHSPALGVHSFSGVQRFNFSSHNQSPKRQSVSHSPNSTSNDSFLAPEVEPIVPELCIDHLWTEAITTMREKNSPASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSVTNLQAKDAAPVEKINTMLVLEGNGNLVLYTGLVRVGKVFIPGLPVPSLTMSNTMPRPSTPLDSVSTPKPLSKPLGSLDEVVLLSPVPELRDSSKVHDSPYIDDCTFQQLGSYIHSVRDPVHNRVTLELSNGSMVRITIPEIATSELVKKCLQAVKSILPKEVAVQMLVKWYNVHNAPGGPSYHSEWNLFVTCLMNMMGYNTDRLSWIRNLDFEGSLSPVIAPKKARPSDTGSDEDWEYLLNSEYHQNVESHLLTRALHLNPLEVSLMKEDVLHNLTLDSSTLLFTHIPAIFFVLHLVYEELKLNSLMGEGIRSLVVLLVQLARDLQLEPYLDYYYRDYPTLVKTSRQSCIIDQAQIGLMHHPSFFTPEPPSIYQWLSSCLKGEGASPYPYLSGVCERSKLIVLSVALYILGDERAVSNEASQYLCKVNSAPKKSQVDQNDNRFSFRHSTPVNSLAEKLVLWMTNVGFTLRDLESLPFGVALPIRDAIYHCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSSVAQVLSSDVPSGTEAEEEDDGMNDMNQEVMSLIWSEDLRVQEVRRLLQSAHPVRVNVVQMPEVNDHEYIEEKENRLLQLCQRTMALPLGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMACWASFHNGVAAGLKIAPASQIDSAWIVYNKPKNAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHNVQVAAVIGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRFQAGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLNDTEIPSSEDLNLETLAQAHVYIIAGACLSLGFRFAGSENLAAFNCLYKFAKDFMKCLSAPTASVTGHYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFMHKKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPVHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALLEVTYKHTVLSSSSFDDTKGTQWYEETTEELMAPTLLPELHLLKQIKVKGPRYWELLIDLSKGIHHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVTHRNSEARAFKPEAISAFTSDPALLSFAEYFCKPTVNLGQKQEILDLFSSILYECVTQENPEMLPAYIAIDQAVRRLERREMSETFELWQIKLVLEFFSSRSHQERMQKIPSRGLFMNSEFLPVMKCTIDNALDQWLQAGGDVCLHSYLSGQPTEESHLSMLACFLIYHSVPTPGQLSAGGLEGSMSFSELLLKFKQLKMPVRALLRLAPLLLGSPQSMVL

Target ORF information:

RefSeq Version	XM_031950991.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031950991.1

ORF Insert Sequence:

ATGTCGAACA TCTATGAAGA GAAGGTAACG ATGATTGCAG CAGGGGATTT GCAGGAGTTT 
GTTCCTTTTG GCCGTGATCA CTGCAAGCAC CACCCTAATG CGCTGAATCT TCAACTTCGG 
CAGCTGCAGC CTGCTTCTGA GCTGTGGTCT TCTGATGGAG CTGCTGGTTT GGTGGGATCC 
CTTCAGGAGG TTACAATCCA TGAAAAACAG AAGGAAAGCT GGCATTTAAG AAAAGGTATA 
AGTGATGTTG GAGAAGAAGT TGAATATGAT GAAGAATTGT ATGTGGCTGG TAATATGGTC 
ATCTGGAGCA AAGGAAGTAA AAGTCAGGCT TCTACTGTTT ATAAAGCTTT TACAGTGGAT 
AGTCCTGTTC TTCAGGCACT CTGGTGTGAC TTCATTATAT CACAGGATAA ATCTGAAAAA 
TATGATAATA GCAATGAAGT AGAAAAATGC ATATGCATAT TACAAAGTTC TTGCATAAAT 
GTGCATAGCA TGGAGGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTC 
TGGCCTACAA AATATGGTTT ATTATTTGAA AGAAGCAGTT CTGCACATGA AGTACCGCCA 
AGTCCACCCA GAGAACCTTT ACCCACTATG TTCAGTATGC TACATCCACT GGATGAAATA 
ACCCCTGTTG TTTGTAAATC TGGAGGTCTT TTTGGAACAT CAAGGGTACA GTATGTTGCT 
GATAGTGCAA TGAAAATTGT TTTTCTAAAT GCTGAGCCTT CTATCATAAT GACATATGAT 
GCTATTCAGG GTTTACATTC TCTCTGGATT CTGCGCAGAG TGAAACCTGA GGAACAGAAT 
GTGGTTTTAA AGTTCTCAGA GCAAGGAGGT ACCCCACAGC ATGTGGCTAC CAGCAGTTCC 
CTTACTGCCC ATCTCAGAAG TCTCTCAAAA GGAGATTCAC CTGTAACTTC ACCTTTTCAG 
AATTATTCTT CCATTCACAG TCAGTCTCGG TCAACATCCT CGCCCAGTTT ACATTCTCGT 
TCACCTTCTA TTTCTAACAT GGCAGCTCTT AGTCGTTCTC ATTCTCCTGC CTTAGGAGTA 
CACTCATTCT CAGGGGTGCA GAGATTCAAC TTTTCAAGCC ATAATCAATC TCCAAAGAGG 
CAGAGTGTTT CTCATTCTCC AAACAGTACT TCTAATGACT CCTTTCTTGC ACCAGAAGTG 
GAGCCAATTG TTCCAGAACT TTGTATTGAC CACTTATGGA CAGAAGCAAT TACCACTATG 
AGAGAGAAAA ATTCTCCAGC TTCAAAAGTA TTTATTACAT CTGACCTCTG TGGCCAGAAG 
TTTTTGTGTT TTTTAGTAGA ATCCCAACTA CAGCTTCGAT GTGTAAAGTT TCAAGAGAGT 
AATGATAAAA CCCAGTTGAT CTTTGGTTCT GTCACTAACT TACAGGCAAA GGATGCAGCA 
CCAGTAGAGA AAATTAATAC CATGCTGGTC CTAGAAGGTA ATGGAAACTT GGTACTATAC 
ACTGGATTGG TTCGGGTAGG GAAAGTTTTC ATTCCTGGAC TACCAGTTCC TTCTCTGACA 
ATGTCAAACA CAATGCCAAG ACCTAGTACT CCCTTGGACA GTGTTAGTAC TCCTAAGCCT 
CTAAGCAAAC CACTTGGCTC CTTGGATGAG GTAGTACTGC TATCTCCAGT TCCAGAACTG 
AGAGATTCTT CAAAAGTTCA TGATTCGCCT TATATTGATG ACTGTACTTT TCAGCAACTT 
GGAAGTTACA TTCACTCTGT TAGAGATCCT GTCCATAACA GAGTAACACT TGAACTCAGT 
AATGGTTCAA TGGTTCGGAT CACCATTCCT GAAATTGCCA CCTCAGAATT AGTAAAAAAG 
TGTTTACAAG CTGTCAAGTC TATACTGCCA AAAGAGGTAG CTGTGCAGAT GCTTGTCAAA 
TGGTACAATG TTCACAATGC TCCAGGAGGA CCCAGTTATC ATTCAGAATG GAATTTATTC 
GTAACTTGTC TAATGAATAT GATGGGCTAT AACACAGACC GCTTATCATG GATCCGTAAT 
CTTGACTTTG AAGGATCTCT TTCTCCAGTC ATAGCACCCA AAAAAGCAAG GCCTTCTGAC 
ACTGGATCAG ATGAGGACTG GGAATATTTA CTAAATTCAG AGTACCACCA GAATGTTGAG 
TCTCATCTTT TGACTAGAGC TTTGCATTTG AATCCTTTGG AAGTTTCACT GATGAAGGAG 
GATGTTTTAC ATAATCTTAC TTTGGATTCT TCTACACTTC TCTTCACTCA TATACCTGCT 
ATCTTTTTTG TGCTTCATCT TGTTTATGAA GAATTAAAAT TGAATAGTTT AATGGGAGAA 
GGAATTCGCT CACTTGTTGT TCTTCTAGTT CAGTTGGCAA GGGATTTACA GTTGGAGCCA 
TATTTAGATT ATTACTATAG AGATTATCCA ACACTTGTGA AAACTTCCCG ACAGTCATGC 
ATAATTGATC AAGCCCAAAT AGGACTTATG CATCACCCGT CATTTTTTAC ACCTGAGCCT 
CCAAGTATAT ATCAGTGGCT GAGTTCTTGC CTAAAGGGTG AAGGAGCATC ACCTTATCCT 
TACCTGTCTG GAGTCTGTGA ACGAAGCAAA CTCATAGTGT TGAGTGTTGC ACTCTATATA 
CTTGGTGATG AGAGAGCTGT TTCTAATGAA GCATCACAAT ATTTATGCAA AGTAAACTCA 
GCACCAAAAA AGAGTCAAGT GGACCAAAAT GACAATAGAT TTAGTTTCAG ACATAGTACA 
CCTGTTAATA GCCTCGCTGA AAAATTAGTA CTCTGGATGA CTAATGTAGG CTTTACCTTG 
AGAGATTTGG AGTCTCTTCC TTTTGGAGTT GCTCTTCCTA TCAGAGATGC TATTTATCAC 
TGCCGAGAAC AGCCTGCTTC AGATTGGCCA GAAGCTGTCT GCCTCTTGAT TGGGCGTCAG 
GATCTATCCA AGCAAGCCTG TGAAGGGAAC TTACCAAAAG GGAAATCTTC TGTGGCTCAG 
GTGCTCTCAT CAGATGTGCC TTCAGGAACT GAAGCAGAGG AAGAAGATGA TGGCATGAAT 
GATATGAATC AGGAAGTCAT GTCACTAATA TGGAGTGAAG ATTTAAGAGT CCAGGAGGTT 
CGAAGACTTC TCCAAAGTGC TCATCCTGTT CGAGTCAATG TAGTACAGAT GCCAGAAGTT 
AATGACCATG AATACATTGA AGAAAAAGAA AACAGGTTGC TCCAGTTGTG TCAGCGAACT 
ATGGCACTTC CCTTGGGGCG TGGAATGTTT ACTTTGTTTT CATACCATCC AGTTCCGACA 
GAACCGTTGC CTGTTCCTAA ACTGAATCTA ACTGGACGGG CTCCTCCTAG GAACACAACT 
GTGGACCTTA ACAGTGGAAA TATTGATGTG CCTCCCAACA TGGCATGTTG GGCTAGTTTT 
CACAATGGTG TTGCTGCGGG GTTAAAAATA GCACCTGCTT CCCAGATTGA CTCAGCTTGG 
ATTGTCTACA ATAAACCAAA GAATGCAGAA TTGGCCAATG AATATGCAGG TTTTCTTATG 
GCCTTAGGCT TGAATGGGCA TCTTACAAAG CTAGCTACTC TTAATATACA TGACTATCTG 
ACCAAGGGCC ATGAGATGAC CAGCATTGGA CTGCTTCTTG GTGTTTCTGC TGCTAAGCTA 
GGCACAATGG ATATGTCAAT TACTCGACTT CTCAGCATCC ATATACCTGC TCTCTTACCT 
CCAACTTCAA CAGAACTTGA TGTCCCTCAC AATGTTCAGG TGGCTGCAGT GATAGGCATA 
GGCCTTGTCT ATCAAGGGAC AGCTCACAGG CATACTGCTG AAGTTCTGCT GGCTGAAATT 
GGACGTCCCC CAGGTCCAGA AATGGAATAT TGCACTGACA GGGAATCATA TTCTTTAGCA 
GCTGGTTTGG CACTGGGCAT GGTTTGCTTG GGGCATGGTA GCAACCTGAT AGGCATGTCT 
GATCTCAATG TACCTGAGCA GCTTTATCAG TATATGGTTG GTGGTCACAG GCGCTTCCAA 
GCAGGAATGC ATAGAGAAAA ACATAAATCT CCAAGTTATC AAATTAAAGA AGGTGACACA 
ATCAATGTAG ATGTGACTTG TCCTGGTGCA ACTTTAGCTT TGGCGATGAT CTACTTAAAA 
ACTAATAACA GATCTATTGC TGACTGGCTT CGAGCTCCTG ATACAATGTA TTTGTTGGAT 
TTTGTGAAAC CAGAATTTCT TCTGCTAAGG ACCCTTGCTC GATGCCTGAT TTTATGGGAT 
GACATTTTGC CTAATTCCAA GTGGGTTGAT AGTAATGTTC CACAAATCAT AAGGGAAAAT 
AGTATTTCTC TTAATGACAC TGAAATACCA TCCTCAGAGG ATCTGAACTT GGAAACTCTG 
GCGCAAGCAC ATGTCTATAT AATTGCTGGA GCTTGTTTGT CTCTGGGTTT CCGATTTGCT 
GGTTCAGAAA ACTTAGCAGC ATTTAATTGT TTGTATAAGT TTGCAAAAGA TTTTATGAAG 
TGTTTGTCTG CACCCACAGC TTCAGTGACA GGCCATTATA ACCTGGAAAC TTGCCTAAGT 
GTCGTGCTCC TCTCTCTTGC CATGGTGATG GCTGGCTCTG GAAATCTGAA GGTTCTCCAG 
CTTTGTCGCT TCATGCATAA GAAGACTGGT GGAGAAATGA ACTATGGTTT CCATTTGGCT 
CATCATATGG CTCTTGGACT TCTCTTTTTA GGAGGAGGCA GATATTCCTT GAGTACTTCA 
AATTCTTCAA TTGCTGCTCT TCTTTGTGCA CTTTATCCCC ACTTTCCAGT TCATAGCACT 
GACAATAGGT ATCATCTCCA GGCTCTTCGA CACTTGTATG TACTAGCAGC AGAACCAAGA 
CTTCTTGTTC CTGTTGATGT AGATACAAAT ACACCTTGCT ATGCATTATT AGAGGTTACA 
TACAAGCACA CTGTACTGAG CTCTTCTTCC TTTGATGATA CAAAGGGTAC TCAGTGGTAT 
GAAGAAACAA CAGAAGAGCT AATGGCACCT ACACTGCTTC CTGAGCTTCA CCTTTTAAAA 
CAGATTAAAG TGAAAGGCCC ACGATACTGG GAATTGCTTA TAGATTTAAG TAAAGGAATT 
CATCACTTGA AATCAATTCT TTCCAAGGAT GGAGTTTTAT ATGTGAAACT CAGGGCTGGA 
CAGCTCTCCT ACAAAGAAGA TCCGATGGGC TGGCAAAGTT TGTTGGCTCA AACAGTCACT 
CATAGAAATT CTGAAGCCAG AGCTTTCAAG CCAGAAGCTA TTTCAGCTTT CACCTCTGAT 
CCAGCACTTT TGTCATTTGC TGAATATTTC TGCAAACCAA CTGTGAACTT GGGCCAGAAA 
CAGGAAATTC TTGATCTCTT TTCTTCAATA CTTTATGAAT GTGTCACGCA GGAGAATCCA 
GAAATGCTGC CTGCATATAT AGCAATAGAT CAGGCTGTCA GAAGACTAGA AAGAAGGGAA 
ATGTCTGAGA CTTTTGAACT TTGGCAAATA AAATTGGTAT TAGAATTCTT TAGCTCTAGA 
AGCCACCAGG AGAGAATGCA GAAGATCCCT AGTCGAGGAC TTTTTATGAA CTCTGAATTC 
CTTCCTGTGA TGAAATGCAC TATTGATAAT GCTTTGGATC AATGGTTACA AGCTGGAGGT 
GATGTGTGCC TGCATTCCTA TCTAAGTGGC CAGCCCACAG AAGAGTCTCA CCTAAGCATG 
TTGGCGTGCT TCCTGATTTA TCATTCTGTT CCTACCCCAG GGCAGCTGTC CGCTGGAGGA 
TTAGAAGGAA GCATGAGTTT TTCTGAGCTA CTTCTAAAAT TCAAACAACT GAAGATGCCA 
GTTCGAGCAT TGCTAAGATT AGCTCCTTTG CTTCTTGGAA GTCCACAATC AATGGTACTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_031950992.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031806852.1 Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa224312
Clone ID Related Accession (Same CDS sequence)	XM_031950991.1 , XM_031950992.1
Accession Version	XM_031950992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	anaphase-promoting complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACA TCTATGAAGA GAAGGTAACG ATGATTGCAG CAGGGGATTT GCAGGAGTTT 
GTTCCTTTTG GCCGTGATCA CTGCAAGCAC CACCCTAATG CGCTGAATCT TCAACTTCGG 
CAGCTGCAGC CTGCTTCTGA GCTGTGGTCT TCTGATGGAG CTGCTGGTTT GGTGGGATCC 
CTTCAGGAGG TTACAATCCA TGAAAAACAG AAGGAAAGCT GGCATTTAAG AAAAGGTATA 
AGTGATGTTG GAGAAGAAGT TGAATATGAT GAAGAATTGT ATGTGGCTGG TAATATGGTC 
ATCTGGAGCA AAGGAAGTAA AAGTCAGGCT TCTACTGTTT ATAAAGCTTT TACAGTGGAT 
AGTCCTGTTC TTCAGGCACT CTGGTGTGAC TTCATTATAT CACAGGATAA ATCTGAAAAA 
TATGATAATA GCAATGAAGT AGAAAAATGC ATATGCATAT TACAAAGTTC TTGCATAAAT 
GTGCATAGCA TGGAGGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTC 
TGGCCTACAA AATATGGTTT ATTATTTGAA AGAAGCAGTT CTGCACATGA AGTACCGCCA 
AGTCCACCCA GAGAACCTTT ACCCACTATG TTCAGTATGC TACATCCACT GGATGAAATA 
ACCCCTGTTG TTTGTAAATC TGGAGGTCTT TTTGGAACAT CAAGGGTACA GTATGTTGCT 
GATAGTGCAA TGAAAATTGT TTTTCTAAAT GCTGAGCCTT CTATCATAAT GACATATGAT 
GCTATTCAGG GTTTACATTC TCTCTGGATT CTGCGCAGAG TGAAACCTGA GGAACAGAAT 
GTGGTTTTAA AGTTCTCAGA GCAAGGAGGT ACCCCACAGC ATGTGGCTAC CAGCAGTTCC 
CTTACTGCCC ATCTCAGAAG TCTCTCAAAA GGAGATTCAC CTGTAACTTC ACCTTTTCAG 
AATTATTCTT CCATTCACAG TCAGTCTCGG TCAACATCCT CGCCCAGTTT ACATTCTCGT 
TCACCTTCTA TTTCTAACAT GGCAGCTCTT AGTCGTTCTC ATTCTCCTGC CTTAGGAGTA 
CACTCATTCT CAGGGGTGCA GAGATTCAAC TTTTCAAGCC ATAATCAATC TCCAAAGAGG 
CAGAGTGTTT CTCATTCTCC AAACAGTACT TCTAATGACT CCTTTCTTGC ACCAGAAGTG 
GAGCCAATTG TTCCAGAACT TTGTATTGAC CACTTATGGA CAGAAGCAAT TACCACTATG 
AGAGAGAAAA ATTCTCCAGC TTCAAAAGTA TTTATTACAT CTGACCTCTG TGGCCAGAAG 
TTTTTGTGTT TTTTAGTAGA ATCCCAACTA CAGCTTCGAT GTGTAAAGTT TCAAGAGAGT 
AATGATAAAA CCCAGTTGAT CTTTGGTTCT GTCACTAACT TACAGGCAAA GGATGCAGCA 
CCAGTAGAGA AAATTAATAC CATGCTGGTC CTAGAAGGTA ATGGAAACTT GGTACTATAC 
ACTGGATTGG TTCGGGTAGG GAAAGTTTTC ATTCCTGGAC TACCAGTTCC TTCTCTGACA 
ATGTCAAACA CAATGCCAAG ACCTAGTACT CCCTTGGACA GTGTTAGTAC TCCTAAGCCT 
CTAAGCAAAC CACTTGGCTC CTTGGATGAG GTAGTACTGC TATCTCCAGT TCCAGAACTG 
AGAGATTCTT CAAAAGTTCA TGATTCGCCT TATATTGATG ACTGTACTTT TCAGCAACTT 
GGAAGTTACA TTCACTCTGT TAGAGATCCT GTCCATAACA GAGTAACACT TGAACTCAGT 
AATGGTTCAA TGGTTCGGAT CACCATTCCT GAAATTGCCA CCTCAGAATT AGTAAAAAAG 
TGTTTACAAG CTGTCAAGTC TATACTGCCA AAAGAGGTAG CTGTGCAGAT GCTTGTCAAA 
TGGTACAATG TTCACAATGC TCCAGGAGGA CCCAGTTATC ATTCAGAATG GAATTTATTC 
GTAACTTGTC TAATGAATAT GATGGGCTAT AACACAGACC GCTTATCATG GATCCGTAAT 
CTTGACTTTG AAGGATCTCT TTCTCCAGTC ATAGCACCCA AAAAAGCAAG GCCTTCTGAC 
ACTGGATCAG ATGAGGACTG GGAATATTTA CTAAATTCAG AGTACCACCA GAATGTTGAG 
TCTCATCTTT TGACTAGAGC TTTGCATTTG AATCCTTTGG AAGTTTCACT GATGAAGGAG 
GATGTTTTAC ATAATCTTAC TTTGGATTCT TCTACACTTC TCTTCACTCA TATACCTGCT 
ATCTTTTTTG TGCTTCATCT TGTTTATGAA GAATTAAAAT TGAATAGTTT AATGGGAGAA 
GGAATTCGCT CACTTGTTGT TCTTCTAGTT CAGTTGGCAA GGGATTTACA GTTGGAGCCA 
TATTTAGATT ATTACTATAG AGATTATCCA ACACTTGTGA AAACTTCCCG ACAGTCATGC 
ATAATTGATC AAGCCCAAAT AGGACTTATG CATCACCCGT CATTTTTTAC ACCTGAGCCT 
CCAAGTATAT ATCAGTGGCT GAGTTCTTGC CTAAAGGGTG AAGGAGCATC ACCTTATCCT 
TACCTGTCTG GAGTCTGTGA ACGAAGCAAA CTCATAGTGT TGAGTGTTGC ACTCTATATA 
CTTGGTGATG AGAGAGCTGT TTCTAATGAA GCATCACAAT ATTTATGCAA AGTAAACTCA 
GCACCAAAAA AGAGTCAAGT GGACCAAAAT GACAATAGAT TTAGTTTCAG ACATAGTACA 
CCTGTTAATA GCCTCGCTGA AAAATTAGTA CTCTGGATGA CTAATGTAGG CTTTACCTTG 
AGAGATTTGG AGTCTCTTCC TTTTGGAGTT GCTCTTCCTA TCAGAGATGC TATTTATCAC 
TGCCGAGAAC AGCCTGCTTC AGATTGGCCA GAAGCTGTCT GCCTCTTGAT TGGGCGTCAG 
GATCTATCCA AGCAAGCCTG TGAAGGGAAC TTACCAAAAG GGAAATCTTC TGTGGCTCAG 
GTGCTCTCAT CAGATGTGCC TTCAGGAACT GAAGCAGAGG AAGAAGATGA TGGCATGAAT 
GATATGAATC AGGAAGTCAT GTCACTAATA TGGAGTGAAG ATTTAAGAGT CCAGGAGGTT 
CGAAGACTTC TCCAAAGTGC TCATCCTGTT CGAGTCAATG TAGTACAGAT GCCAGAAGTT 
AATGACCATG AATACATTGA AGAAAAAGAA AACAGGTTGC TCCAGTTGTG TCAGCGAACT 
ATGGCACTTC CCTTGGGGCG TGGAATGTTT ACTTTGTTTT CATACCATCC AGTTCCGACA 
GAACCGTTGC CTGTTCCTAA ACTGAATCTA ACTGGACGGG CTCCTCCTAG GAACACAACT 
GTGGACCTTA ACAGTGGAAA TATTGATGTG CCTCCCAACA TGGCATGTTG GGCTAGTTTT 
CACAATGGTG TTGCTGCGGG GTTAAAAATA GCACCTGCTT CCCAGATTGA CTCAGCTTGG 
ATTGTCTACA ATAAACCAAA GAATGCAGAA TTGGCCAATG AATATGCAGG TTTTCTTATG 
GCCTTAGGCT TGAATGGGCA TCTTACAAAG CTAGCTACTC TTAATATACA TGACTATCTG 
ACCAAGGGCC ATGAGATGAC CAGCATTGGA CTGCTTCTTG GTGTTTCTGC TGCTAAGCTA 
GGCACAATGG ATATGTCAAT TACTCGACTT CTCAGCATCC ATATACCTGC TCTCTTACCT 
CCAACTTCAA CAGAACTTGA TGTCCCTCAC AATGTTCAGG TGGCTGCAGT GATAGGCATA 
GGCCTTGTCT ATCAAGGGAC AGCTCACAGG CATACTGCTG AAGTTCTGCT GGCTGAAATT 
GGACGTCCCC CAGGTCCAGA AATGGAATAT TGCACTGACA GGGAATCATA TTCTTTAGCA 
GCTGGTTTGG CACTGGGCAT GGTTTGCTTG GGGCATGGTA GCAACCTGAT AGGCATGTCT 
GATCTCAATG TACCTGAGCA GCTTTATCAG TATATGGTTG GTGGTCACAG GCGCTTCCAA 
GCAGGAATGC ATAGAGAAAA ACATAAATCT CCAAGTTATC AAATTAAAGA AGGTGACACA 
ATCAATGTAG ATGTGACTTG TCCTGGTGCA ACTTTAGCTT TGGCGATGAT CTACTTAAAA 
ACTAATAACA GATCTATTGC TGACTGGCTT CGAGCTCCTG ATACAATGTA TTTGTTGGAT 
TTTGTGAAAC CAGAATTTCT TCTGCTAAGG ACCCTTGCTC GATGCCTGAT TTTATGGGAT 
GACATTTTGC CTAATTCCAA GTGGGTTGAT AGTAATGTTC CACAAATCAT AAGGGAAAAT 
AGTATTTCTC TTAATGACAC TGAAATACCA TCCTCAGAGG ATCTGAACTT GGAAACTCTG 
GCGCAAGCAC ATGTCTATAT AATTGCTGGA GCTTGTTTGT CTCTGGGTTT CCGATTTGCT 
GGTTCAGAAA ACTTAGCAGC ATTTAATTGT TTGTATAAGT TTGCAAAAGA TTTTATGAAG 
TGTTTGTCTG CACCCACAGC TTCAGTGACA GGCCATTATA ACCTGGAAAC TTGCCTAAGT 
GTCGTGCTCC TCTCTCTTGC CATGGTGATG GCTGGCTCTG GAAATCTGAA GGTTCTCCAG 
CTTTGTCGCT TCATGCATAA GAAGACTGGT GGAGAAATGA ACTATGGTTT CCATTTGGCT 
CATCATATGG CTCTTGGACT TCTCTTTTTA GGAGGAGGCA GATATTCCTT GAGTACTTCA 
AATTCTTCAA TTGCTGCTCT TCTTTGTGCA CTTTATCCCC ACTTTCCAGT TCATAGCACT 
GACAATAGGT ATCATCTCCA GGCTCTTCGA CACTTGTATG TACTAGCAGC AGAACCAAGA 
CTTCTTGTTC CTGTTGATGT AGATACAAAT ACACCTTGCT ATGCATTATT AGAGGTTACA 
TACAAGCACA CTGTACTGAG CTCTTCTTCC TTTGATGATA CAAAGGGTAC TCAGTGGTAT 
GAAGAAACAA CAGAAGAGCT AATGGCACCT ACACTGCTTC CTGAGCTTCA CCTTTTAAAA 
CAGATTAAAG TGAAAGGCCC ACGATACTGG GAATTGCTTA TAGATTTAAG TAAAGGAATT 
CATCACTTGA AATCAATTCT TTCCAAGGAT GGAGTTTTAT ATGTGAAACT CAGGGCTGGA 
CAGCTCTCCT ACAAAGAAGA TCCGATGGGC TGGCAAAGTT TGTTGGCTCA AACAGTCACT 
CATAGAAATT CTGAAGCCAG AGCTTTCAAG CCAGAAGCTA TTTCAGCTTT CACCTCTGAT 
CCAGCACTTT TGTCATTTGC TGAATATTTC TGCAAACCAA CTGTGAACTT GGGCCAGAAA 
CAGGAAATTC TTGATCTCTT TTCTTCAATA CTTTATGAAT GTGTCACGCA GGAGAATCCA 
GAAATGCTGC CTGCATATAT AGCAATAGAT CAGGCTGTCA GAAGACTAGA AAGAAGGGAA 
ATGTCTGAGA CTTTTGAACT TTGGCAAATA AAATTGGTAT TAGAATTCTT TAGCTCTAGA 
AGCCACCAGG AGAGAATGCA GAAGATCCCT AGTCGAGGAC TTTTTATGAA CTCTGAATTC 
CTTCCTGTGA TGAAATGCAC TATTGATAAT GCTTTGGATC AATGGTTACA AGCTGGAGGT 
GATGTGTGCC TGCATTCCTA TCTAAGTGGC CAGCCCACAG AAGAGTCTCA CCTAAGCATG 
TTGGCGTGCT TCCTGATTTA TCATTCTGTT CCTACCCCAG GGCAGCTGTC CGCTGGAGGA 
TTAGAAGGAA GCATGAGTTT TTCTGAGCTA CTTCTAAAAT TCAAACAACT GAAGATGCCA 
GTTCGAGCAT TGCTAAGATT AGCTCCTTTG CTTCTTGGAA GTCCACAATC AATGGTACTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031806852.1
CDS	362..6244
Translation	MSNIYEEKVTMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWHLRKGISDVGEEVEYDEELYVAGNMVIWSKGSKSQASTVYKAFTVDSPVLQALWCDFIISQDKSEKYDNSNEVEKCICILQSSCINVHSMEGKDYIASLPFQVANVWPTKYGLLFERSSSAHEVPPSPPREPLPTMFSMLHPLDEITPVVCKSGGLFGTSRVQYVADSAMKIVFLNAEPSIIMTYDAIQGLHSLWILRRVKPEEQNVVLKFSEQGGTPQHVATSSSLTAHLRSLSKGDSPVTSPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRSHSPALGVHSFSGVQRFNFSSHNQSPKRQSVSHSPNSTSNDSFLAPEVEPIVPELCIDHLWTEAITTMREKNSPASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSVTNLQAKDAAPVEKINTMLVLEGNGNLVLYTGLVRVGKVFIPGLPVPSLTMSNTMPRPSTPLDSVSTPKPLSKPLGSLDEVVLLSPVPELRDSSKVHDSPYIDDCTFQQLGSYIHSVRDPVHNRVTLELSNGSMVRITIPEIATSELVKKCLQAVKSILPKEVAVQMLVKWYNVHNAPGGPSYHSEWNLFVTCLMNMMGYNTDRLSWIRNLDFEGSLSPVIAPKKARPSDTGSDEDWEYLLNSEYHQNVESHLLTRALHLNPLEVSLMKEDVLHNLTLDSSTLLFTHIPAIFFVLHLVYEELKLNSLMGEGIRSLVVLLVQLARDLQLEPYLDYYYRDYPTLVKTSRQSCIIDQAQIGLMHHPSFFTPEPPSIYQWLSSCLKGEGASPYPYLSGVCERSKLIVLSVALYILGDERAVSNEASQYLCKVNSAPKKSQVDQNDNRFSFRHSTPVNSLAEKLVLWMTNVGFTLRDLESLPFGVALPIRDAIYHCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSSVAQVLSSDVPSGTEAEEEDDGMNDMNQEVMSLIWSEDLRVQEVRRLLQSAHPVRVNVVQMPEVNDHEYIEEKENRLLQLCQRTMALPLGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMACWASFHNGVAAGLKIAPASQIDSAWIVYNKPKNAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHNVQVAAVIGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRFQAGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLNDTEIPSSEDLNLETLAQAHVYIIAGACLSLGFRFAGSENLAAFNCLYKFAKDFMKCLSAPTASVTGHYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFMHKKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPVHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALLEVTYKHTVLSSSSFDDTKGTQWYEETTEELMAPTLLPELHLLKQIKVKGPRYWELLIDLSKGIHHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVTHRNSEARAFKPEAISAFTSDPALLSFAEYFCKPTVNLGQKQEILDLFSSILYECVTQENPEMLPAYIAIDQAVRRLERREMSETFELWQIKLVLEFFSSRSHQERMQKIPSRGLFMNSEFLPVMKCTIDNALDQWLQAGGDVCLHSYLSGQPTEESHLSMLACFLIYHSVPTPGQLSAGGLEGSMSFSELLLKFKQLKMPVRALLRLAPLLLGSPQSMVL

Target ORF information:

RefSeq Version	XM_031950992.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii anaphase promoting complex subunit 1 (ANAPC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031950992.1

ORF Insert Sequence:

ATGTCGAACA TCTATGAAGA GAAGGTAACG ATGATTGCAG CAGGGGATTT GCAGGAGTTT 
GTTCCTTTTG GCCGTGATCA CTGCAAGCAC CACCCTAATG CGCTGAATCT TCAACTTCGG 
CAGCTGCAGC CTGCTTCTGA GCTGTGGTCT TCTGATGGAG CTGCTGGTTT GGTGGGATCC 
CTTCAGGAGG TTACAATCCA TGAAAAACAG AAGGAAAGCT GGCATTTAAG AAAAGGTATA 
AGTGATGTTG GAGAAGAAGT TGAATATGAT GAAGAATTGT ATGTGGCTGG TAATATGGTC 
ATCTGGAGCA AAGGAAGTAA AAGTCAGGCT TCTACTGTTT ATAAAGCTTT TACAGTGGAT 
AGTCCTGTTC TTCAGGCACT CTGGTGTGAC TTCATTATAT CACAGGATAA ATCTGAAAAA 
TATGATAATA GCAATGAAGT AGAAAAATGC ATATGCATAT TACAAAGTTC TTGCATAAAT 
GTGCATAGCA TGGAGGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTC 
TGGCCTACAA AATATGGTTT ATTATTTGAA AGAAGCAGTT CTGCACATGA AGTACCGCCA 
AGTCCACCCA GAGAACCTTT ACCCACTATG TTCAGTATGC TACATCCACT GGATGAAATA 
ACCCCTGTTG TTTGTAAATC TGGAGGTCTT TTTGGAACAT CAAGGGTACA GTATGTTGCT 
GATAGTGCAA TGAAAATTGT TTTTCTAAAT GCTGAGCCTT CTATCATAAT GACATATGAT 
GCTATTCAGG GTTTACATTC TCTCTGGATT CTGCGCAGAG TGAAACCTGA GGAACAGAAT 
GTGGTTTTAA AGTTCTCAGA GCAAGGAGGT ACCCCACAGC ATGTGGCTAC CAGCAGTTCC 
CTTACTGCCC ATCTCAGAAG TCTCTCAAAA GGAGATTCAC CTGTAACTTC ACCTTTTCAG 
AATTATTCTT CCATTCACAG TCAGTCTCGG TCAACATCCT CGCCCAGTTT ACATTCTCGT 
TCACCTTCTA TTTCTAACAT GGCAGCTCTT AGTCGTTCTC ATTCTCCTGC CTTAGGAGTA 
CACTCATTCT CAGGGGTGCA GAGATTCAAC TTTTCAAGCC ATAATCAATC TCCAAAGAGG 
CAGAGTGTTT CTCATTCTCC AAACAGTACT TCTAATGACT CCTTTCTTGC ACCAGAAGTG 
GAGCCAATTG TTCCAGAACT TTGTATTGAC CACTTATGGA CAGAAGCAAT TACCACTATG 
AGAGAGAAAA ATTCTCCAGC TTCAAAAGTA TTTATTACAT CTGACCTCTG TGGCCAGAAG 
TTTTTGTGTT TTTTAGTAGA ATCCCAACTA CAGCTTCGAT GTGTAAAGTT TCAAGAGAGT 
AATGATAAAA CCCAGTTGAT CTTTGGTTCT GTCACTAACT TACAGGCAAA GGATGCAGCA 
CCAGTAGAGA AAATTAATAC CATGCTGGTC CTAGAAGGTA ATGGAAACTT GGTACTATAC 
ACTGGATTGG TTCGGGTAGG GAAAGTTTTC ATTCCTGGAC TACCAGTTCC TTCTCTGACA 
ATGTCAAACA CAATGCCAAG ACCTAGTACT CCCTTGGACA GTGTTAGTAC TCCTAAGCCT 
CTAAGCAAAC CACTTGGCTC CTTGGATGAG GTAGTACTGC TATCTCCAGT TCCAGAACTG 
AGAGATTCTT CAAAAGTTCA TGATTCGCCT TATATTGATG ACTGTACTTT TCAGCAACTT 
GGAAGTTACA TTCACTCTGT TAGAGATCCT GTCCATAACA GAGTAACACT TGAACTCAGT 
AATGGTTCAA TGGTTCGGAT CACCATTCCT GAAATTGCCA CCTCAGAATT AGTAAAAAAG 
TGTTTACAAG CTGTCAAGTC TATACTGCCA AAAGAGGTAG CTGTGCAGAT GCTTGTCAAA 
TGGTACAATG TTCACAATGC TCCAGGAGGA CCCAGTTATC ATTCAGAATG GAATTTATTC 
GTAACTTGTC TAATGAATAT GATGGGCTAT AACACAGACC GCTTATCATG GATCCGTAAT 
CTTGACTTTG AAGGATCTCT TTCTCCAGTC ATAGCACCCA AAAAAGCAAG GCCTTCTGAC 
ACTGGATCAG ATGAGGACTG GGAATATTTA CTAAATTCAG AGTACCACCA GAATGTTGAG 
TCTCATCTTT TGACTAGAGC TTTGCATTTG AATCCTTTGG AAGTTTCACT GATGAAGGAG 
GATGTTTTAC ATAATCTTAC TTTGGATTCT TCTACACTTC TCTTCACTCA TATACCTGCT 
ATCTTTTTTG TGCTTCATCT TGTTTATGAA GAATTAAAAT TGAATAGTTT AATGGGAGAA 
GGAATTCGCT CACTTGTTGT TCTTCTAGTT CAGTTGGCAA GGGATTTACA GTTGGAGCCA 
TATTTAGATT ATTACTATAG AGATTATCCA ACACTTGTGA AAACTTCCCG ACAGTCATGC 
ATAATTGATC AAGCCCAAAT AGGACTTATG CATCACCCGT CATTTTTTAC ACCTGAGCCT 
CCAAGTATAT ATCAGTGGCT GAGTTCTTGC CTAAAGGGTG AAGGAGCATC ACCTTATCCT 
TACCTGTCTG GAGTCTGTGA ACGAAGCAAA CTCATAGTGT TGAGTGTTGC ACTCTATATA 
CTTGGTGATG AGAGAGCTGT TTCTAATGAA GCATCACAAT ATTTATGCAA AGTAAACTCA 
GCACCAAAAA AGAGTCAAGT GGACCAAAAT GACAATAGAT TTAGTTTCAG ACATAGTACA 
CCTGTTAATA GCCTCGCTGA AAAATTAGTA CTCTGGATGA CTAATGTAGG CTTTACCTTG 
AGAGATTTGG AGTCTCTTCC TTTTGGAGTT GCTCTTCCTA TCAGAGATGC TATTTATCAC 
TGCCGAGAAC AGCCTGCTTC AGATTGGCCA GAAGCTGTCT GCCTCTTGAT TGGGCGTCAG 
GATCTATCCA AGCAAGCCTG TGAAGGGAAC TTACCAAAAG GGAAATCTTC TGTGGCTCAG 
GTGCTCTCAT CAGATGTGCC TTCAGGAACT GAAGCAGAGG AAGAAGATGA TGGCATGAAT 
GATATGAATC AGGAAGTCAT GTCACTAATA TGGAGTGAAG ATTTAAGAGT CCAGGAGGTT 
CGAAGACTTC TCCAAAGTGC TCATCCTGTT CGAGTCAATG TAGTACAGAT GCCAGAAGTT 
AATGACCATG AATACATTGA AGAAAAAGAA AACAGGTTGC TCCAGTTGTG TCAGCGAACT 
ATGGCACTTC CCTTGGGGCG TGGAATGTTT ACTTTGTTTT CATACCATCC AGTTCCGACA 
GAACCGTTGC CTGTTCCTAA ACTGAATCTA ACTGGACGGG CTCCTCCTAG GAACACAACT 
GTGGACCTTA ACAGTGGAAA TATTGATGTG CCTCCCAACA TGGCATGTTG GGCTAGTTTT 
CACAATGGTG TTGCTGCGGG GTTAAAAATA GCACCTGCTT CCCAGATTGA CTCAGCTTGG 
ATTGTCTACA ATAAACCAAA GAATGCAGAA TTGGCCAATG AATATGCAGG TTTTCTTATG 
GCCTTAGGCT TGAATGGGCA TCTTACAAAG CTAGCTACTC TTAATATACA TGACTATCTG 
ACCAAGGGCC ATGAGATGAC CAGCATTGGA CTGCTTCTTG GTGTTTCTGC TGCTAAGCTA 
GGCACAATGG ATATGTCAAT TACTCGACTT CTCAGCATCC ATATACCTGC TCTCTTACCT 
CCAACTTCAA CAGAACTTGA TGTCCCTCAC AATGTTCAGG TGGCTGCAGT GATAGGCATA 
GGCCTTGTCT ATCAAGGGAC AGCTCACAGG CATACTGCTG AAGTTCTGCT GGCTGAAATT 
GGACGTCCCC CAGGTCCAGA AATGGAATAT TGCACTGACA GGGAATCATA TTCTTTAGCA 
GCTGGTTTGG CACTGGGCAT GGTTTGCTTG GGGCATGGTA GCAACCTGAT AGGCATGTCT 
GATCTCAATG TACCTGAGCA GCTTTATCAG TATATGGTTG GTGGTCACAG GCGCTTCCAA 
GCAGGAATGC ATAGAGAAAA ACATAAATCT CCAAGTTATC AAATTAAAGA AGGTGACACA 
ATCAATGTAG ATGTGACTTG TCCTGGTGCA ACTTTAGCTT TGGCGATGAT CTACTTAAAA 
ACTAATAACA GATCTATTGC TGACTGGCTT CGAGCTCCTG ATACAATGTA TTTGTTGGAT 
TTTGTGAAAC CAGAATTTCT TCTGCTAAGG ACCCTTGCTC GATGCCTGAT TTTATGGGAT 
GACATTTTGC CTAATTCCAA GTGGGTTGAT AGTAATGTTC CACAAATCAT AAGGGAAAAT 
AGTATTTCTC TTAATGACAC TGAAATACCA TCCTCAGAGG ATCTGAACTT GGAAACTCTG 
GCGCAAGCAC ATGTCTATAT AATTGCTGGA GCTTGTTTGT CTCTGGGTTT CCGATTTGCT 
GGTTCAGAAA ACTTAGCAGC ATTTAATTGT TTGTATAAGT TTGCAAAAGA TTTTATGAAG 
TGTTTGTCTG CACCCACAGC TTCAGTGACA GGCCATTATA ACCTGGAAAC TTGCCTAAGT 
GTCGTGCTCC TCTCTCTTGC CATGGTGATG GCTGGCTCTG GAAATCTGAA GGTTCTCCAG 
CTTTGTCGCT TCATGCATAA GAAGACTGGT GGAGAAATGA ACTATGGTTT CCATTTGGCT 
CATCATATGG CTCTTGGACT TCTCTTTTTA GGAGGAGGCA GATATTCCTT GAGTACTTCA 
AATTCTTCAA TTGCTGCTCT TCTTTGTGCA CTTTATCCCC ACTTTCCAGT TCATAGCACT 
GACAATAGGT ATCATCTCCA GGCTCTTCGA CACTTGTATG TACTAGCAGC AGAACCAAGA 
CTTCTTGTTC CTGTTGATGT AGATACAAAT ACACCTTGCT ATGCATTATT AGAGGTTACA 
TACAAGCACA CTGTACTGAG CTCTTCTTCC TTTGATGATA CAAAGGGTAC TCAGTGGTAT 
GAAGAAACAA CAGAAGAGCT AATGGCACCT ACACTGCTTC CTGAGCTTCA CCTTTTAAAA 
CAGATTAAAG TGAAAGGCCC ACGATACTGG GAATTGCTTA TAGATTTAAG TAAAGGAATT 
CATCACTTGA AATCAATTCT TTCCAAGGAT GGAGTTTTAT ATGTGAAACT CAGGGCTGGA 
CAGCTCTCCT ACAAAGAAGA TCCGATGGGC TGGCAAAGTT TGTTGGCTCA AACAGTCACT 
CATAGAAATT CTGAAGCCAG AGCTTTCAAG CCAGAAGCTA TTTCAGCTTT CACCTCTGAT 
CCAGCACTTT TGTCATTTGC TGAATATTTC TGCAAACCAA CTGTGAACTT GGGCCAGAAA 
CAGGAAATTC TTGATCTCTT TTCTTCAATA CTTTATGAAT GTGTCACGCA GGAGAATCCA 
GAAATGCTGC CTGCATATAT AGCAATAGAT CAGGCTGTCA GAAGACTAGA AAGAAGGGAA 
ATGTCTGAGA CTTTTGAACT TTGGCAAATA AAATTGGTAT TAGAATTCTT TAGCTCTAGA 
AGCCACCAGG AGAGAATGCA GAAGATCCCT AGTCGAGGAC TTTTTATGAA CTCTGAATTC 
CTTCCTGTGA TGAAATGCAC TATTGATAAT GCTTTGGATC AATGGTTACA AGCTGGAGGT 
GATGTGTGCC TGCATTCCTA TCTAAGTGGC CAGCCCACAG AAGAGTCTCA CCTAAGCATG 
TTGGCGTGCT TCCTGATTTA TCATTCTGTT CCTACCCCAG GGCAGCTGTC CGCTGGAGGA 
TTAGAAGGAA GCATGAGTTT TTCTGAGCTA CTTCTAAAAT TCAAACAACT GAAGATGCCA 
GTTCGAGCAT TGCTAAGATT AGCTCCTTTG CTTCTTGGAA GTCCACAATC AATGGTACTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ANAPC1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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