LOC100932280 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100932280
Entrez Gene ID	100932280
Full Name	cullin-7-like
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031968620.1	XP_031824480.1	cullin-7
XM_023503012.1	XP_023358780.1	cullin-7-like
XM_012549054.1	XP_012404508.1	cullin-7-like

Pathways from BioSystems
Gene Ontology

The following LOC100932280 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100932280 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa131207	XM_012549054.1 Latest version!	Sarcophilus harrisii cullin-7-like (LOC100932280), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSa154826	XM_023503012.1 Latest version!	Sarcophilus harrisii cullin-7-like (LOC100932280), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa235352	XM_031968620.1 Latest version!	Sarcophilus harrisii cullin 7 (CUL7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100932280 ( XM_012549054.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012404508.1 Sarcophilus harrisii cullin-7-like (LOC100932280), mRNA.

Price & Availability↑

CloneID	OSa131207
Clone ID Related Accession (Same CDS sequence)	XM_012549054.1
Accession Version	XM_012549054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	cullin-7-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838897.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

ATGGGTTCGC CCCATCTGCT CCTTTCACCC CCACCTAAGA CCTTCGGCTT TCCAGCGGTT 
AAACGCGGTC TGGACGAAGT GCTCTTCCAG TCCGGGATCA TTCCGAGTCC TCTCCTCCCC 
GTGGCAGAGC TATCCCAGAT CTGGGGATCT GACCCTCGCG CCCAACCCCG GTCCAGTCAG 
TACACACCTC CTCCTGGTCC GGGAGCGTCT CGGCGCCGGA GTGGCATTAC GCAGGTGCTG 
TGGCAGTCGA CAGGCCGCAC ATACTGGGTG CACTGGCACA TGGTGGAGAT TCTGGGTCCT 
GTAACTGAGG ACAAAGTTAC TATTGAGCCA GAGAAAGGAA CTGGGGGCAG CAGGTGCCTG 
GCCCCAAGTA TAGCACGTCC ATTCTTAGAG TGGAAACCTA TTGTGGGACT GTATCCTCTG 
CCGTACCTTA AACCTGAGGC TCAGAGCAAC ACAGAGTCTG GGTGCCTGAG CCAGGCCGAA 
TGGTGGGAAC TTCTTTTCTT CATCAAAAAG CTGGATGGGC CTGAGCAACA GGAGTTTCTC 
CGGCTCCTCC AGGAGAACCC GGATGGGGAG CCTGTGGAAG AAGAAGCCCT TGGTGAACTC 
AAAGTATCTG TGGAGTTGGC AGAGAAACTG GTCCTGGCAC TCAGCAGGCA GGTTCAAGGC 
AGCATCCACA ATGACCTGCT TAGTTCGATT GTTTACAACA AATTCAGGTT ACAGCAGGTG 
GAGGATTTGG CCAACCCTAC TCCTCCAGAG CCTGGTCAGG ACACCTGCAG CCCTGAGGCC 
CCATTTCCAG ACACCAAATC AGAAGTGGAG GTGAAAGGGC AGATTAAATC ACTAGGGGTG 
GAGGAGTCAT CAACAGCACC GGAAGTTGAG CTGCTGTTTC GGAGCCTCGT GGGGGCTGCA 
GAGCCCTCTG GACAGCTGCT CCTGGAGCTG GAGCTAAGCA TATGCCAGGA GGGCACTGGG 
GAGGGTCAAG TGAGCCAGCT CCTGGGGCAG CTCCAACACC AGCCCCAAGC CTTCCTGCTG 
CTACTACAGA GCCTGGAGAC CCCCGAAAAC AAGGCCCTTC AAATGACTGG GCTGAGAATA 
CTGAGCTGTC TCCTGGATGT CTCTGTGGAC TCAAGTCTTC CTTGGCACAA AGTGGCCTCT 
TCCTGTTTGG CCAGCCTGAG AACACTCAGC AAAGACCATG AGGTGGTCCA AGGGTTAATC 
TGTTTCCTGC ATCGCCTGAT CACACTACAC AAGGACTGCG CAGTGGTTCT GTACCAGCTC 
GGAGCTCAAG AGATTCTCAC CAAGGCCCTG GACAAGCACA AGGAACAACT GTTTCTGGGC 
TCTGAGCTCC AAGATCTAGT GGGAGAGTAT GAGAAGCACG CCCAGCTTTA CAGCAACCTC 
ACCAAAAGCA TCTTGGCCGG CTGCATCCAG ATGGTGCTCG GCCAGATTGA AGAGCACCGA 
AGAACCCACC GGCCCATTAA CATCCCTTTC TTTGACGTCT TCCTCAGGCA TCTTTGCCAG 
GGCTCTGATG TGGAGATGAA GGACAAGTGT TGGGAGAAGA TGGAGGTGTC GTCTAATTCT 
CTCGGGGCCA ACAAACTGAC TGACCGAAAC CCCAAGACTT ACTGGGAATC CAGCGGCACT 
ACAGGGTCTC ACTACATCAC ATTACACATG CATCAAGGGG CCATTGTCAG ACAGCTGACT 
CTCCTGGTGT CTAAGGAAGA CTCAAGCTAC ATGCCGGCCC GGGTGGTGGT GCTGGGGGGA 
GACCATGCCC GCTCTGTCAG CACAAAGCTC AGCACTGTAA ATGTCATGCC TTCTGACACG 
CGGGTGCTTC TTCTGGAGAA CATGACCCGC TTCTGGCCCA TCCTACAGAT CCATATCAAG 
CGCTGCCAGC AGGGTGGCAT TGACACCCGT GTGCGAGGGG TGGAGGTTCT GGGTCCCAAA 
CCCACTTTCT GGCCACTGTT CCGTGAGCAG CTGTGTCGCC GCACTCACCT TTTCTACACC 
ATACGAGCCC AAGCCTGGAG TCAGGATATT GCTCAGGACC GGAAGCGCCT GCTGCAGCTC 
TGCCCTAGAC TGAACGGGGC TTTGCGCCAT GAGCAGAGTT TTGCAGATCG ATTTCTTCCT 
GATGATGAGG CTGCCCAGGC ACTGGGAAGG ACCTGCTGGG AAGCCTTGGT CAGCCCCTTG 
GTGCAGAGCA TCACTGCCCC TGATGGCAGT GGGGTAAGCC CCCTGGCCTG GCTGCTGAGC 
CAGTATCTGG AGCACAGAGA GAAGGTCCAG CACAAACAGG AACAAGCAGA TGCTTTTACC 
TCACGTGTCC GATGTCTCAC TCACCTGCTG GTGCATGTGG AGCCTCCCTC GGGTCTCCAG 
TCCAAGTTAC TCACCTGGCC TAATGGCAAG AATAGTAAGA GTCGGGAGCT GAACTCGGGG 
GCTGGACTGG GACCTCCTGG TGGCAGCCTG AGGGGTATCA CCCAGTGTTG GAGGGGAGTG 
GTACAAGAGC AGGTGAGCAG GTTTCTGACT GCGGCCTGGC AGGCCCCGGA CCTGGTGCCC 
AGCTACTGCG ACATCTACGA ACGATTTTAT TATGTGTTTG TTAAATTGGA CCGACTTGAA 
GGAGGAAGGT CCCTGAGAGA CATTTGTCCC CACAGCCAGT GGAGGAGCTG GTGGCTGAGG 
AGCCAGTCCC TGAAGTGTCT GTGCTGGTCC TGTCTCCCCG CTGCTGGCCC GTGTCCCCCC 
TCTGCTACCT GCACGAGCCT ACCAAGTACT TCCCCCCGGC TCTCAGCACC AGCCTCACCC 
GGTTTTCCAA CTTCTACAGT CAGAGAGGTG GCCTTGGAGA CGCTGCTGCA GACCTCAGAC 
CTGCCCTCAG AGCTTCTGAT GCAAGCACTT AAGCCCCTCA CTTCAGGGCT TGGCCCCCTG 
ACCCTCCAGG AAGGCCAGGG TCTCCCACCC GGGGGTGTGC TGCGGCTTCC GGAGGTGGAG 
GAGGAGCCTG GGGGGGAGGC CCTGTGGGGC GGGGCCGCGG GACCAGGTAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012404508.1
CDS	1..2997
Translation	MGSPHLLLSPPPKTFGFPAVKRGLDEVLFQSGIIPSPLLPVAELSQIWGSDPRAQPRSSQYTPPPGPGASRRRSGITQVLWQSTGRTYWVHWHMVEILGPVTEDKVTIEPEKGTGGSRCLAPSIARPFLEWKPIVGLYPLPYLKPEAQSNTESGCLSQAEWWELLFFIKKLDGPEQQEFLRLLQENPDGEPVEEEALGELKVSVELAEKLVLALSRQVQGSIHNDLLSSIVYNKFRLQQVEDLANPTPPEPGQDTCSPEAPFPDTKSEVEVKGQIKSLGVEESSTAPEVELLFRSLVGAAEPSGQLLLELELSICQEGTGEGQVSQLLGQLQHQPQAFLLLLQSLETPENKALQMTGLRILSCLLDVSVDSSLPWHKVASSCLASLRTLSKDHEVVQGLICFLHRLITLHKDCAVVLYQLGAQEILTKALDKHKEQLFLGSELQDLVGEYEKHAQLYSNLTKSILAGCIQMVLGQIEEHRRTHRPINIPFFDVFLRHLCQGSDVEMKDKCWEKMEVSSNSLGANKLTDRNPKTYWESSGTTGSHYITLHMHQGAIVRQLTLLVSKEDSSYMPARVVVLGGDHARSVSTKLSTVNVMPSDTRVLLLENMTRFWPILQIHIKRCQQGGIDTRVRGVEVLGPKPTFWPLFREQLCRRTHLFYTIRAQAWSQDIAQDRKRLLQLCPRLNGALRHEQSFADRFLPDDEAAQALGRTCWEALVSPLVQSITAPDGSGVSPLAWLLSQYLEHREKVQHKQEQADAFTSRVRCLTHLLVHVEPPSGLQSKLLTWPNGKNSKSRELNSGAGLGPPGGSLRGITQCWRGVVQEQVSRFLTAAWQAPDLVPSYCDIYERFYYVFVKLDRLEGGRSLRDICPHSQWRSWWLRSQSLKCLCWSCLPAAGPCPPSATCTSLPSTSPRLSAPASPGFPTSTVREVALETLLQTSDLPSELLMQALKPLTSGLGPLTLQEGQGLPPGGVLRLPEVEEEPGGEALWGGAAGPGIS

Target ORF information:

RefSeq Version	XM_012549054.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii cullin-7-like (LOC100932280), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012549054.1

ORF Insert Sequence:

ATGGGTTCGC CCCATCTGCT CCTTTCACCC CCACCTAAGA CCTTCGGCTT TCCAGCGGTT 
AAACGCGGTC TGGACGAAGT GCTCTTCCAG TCCGGGATCA TTCCGAGTCC TCTCCTCCCC 
GTGGCAGAGC TATCCCAGAT CTGGGGATCT GACCCTCGCG CCCAACCCCG GTCCAGTCAG 
TACACACCTC CTCCTGGTCC GGGAGCGTCT CGGCGCCGGA GTGGCATTAC GCAGGTGCTG 
TGGCAGTCGA CAGGCCGCAC ATACTGGGTG CACTGGCACA TGGTGGAGAT TCTGGGTCCT 
GTAACTGAGG ACAAAGTTAC TATTGAGCCA GAGAAAGGAA CTGGGGGCAG CAGGTGCCTG 
GCCCCAAGTA TAGCACGTCC ATTCTTAGAG TGGAAACCTA TTGTGGGACT GTATCCTCTG 
CCGTACCTTA AACCTGAGGC TCAGAGCAAC ACAGAGTCTG GGTGCCTGAG CCAGGCCGAA 
TGGTGGGAAC TTCTTTTCTT CATCAAAAAG CTGGATGGGC CTGAGCAACA GGAGTTTCTC 
CGGCTCCTCC AGGAGAACCC GGATGGGGAG CCTGTGGAAG AAGAAGCCCT TGGTGAACTC 
AAAGTATCTG TGGAGTTGGC AGAGAAACTG GTCCTGGCAC TCAGCAGGCA GGTTCAAGGC 
AGCATCCACA ATGACCTGCT TAGTTCGATT GTTTACAACA AATTCAGGTT ACAGCAGGTG 
GAGGATTTGG CCAACCCTAC TCCTCCAGAG CCTGGTCAGG ACACCTGCAG CCCTGAGGCC 
CCATTTCCAG ACACCAAATC AGAAGTGGAG GTGAAAGGGC AGATTAAATC ACTAGGGGTG 
GAGGAGTCAT CAACAGCACC GGAAGTTGAG CTGCTGTTTC GGAGCCTCGT GGGGGCTGCA 
GAGCCCTCTG GACAGCTGCT CCTGGAGCTG GAGCTAAGCA TATGCCAGGA GGGCACTGGG 
GAGGGTCAAG TGAGCCAGCT CCTGGGGCAG CTCCAACACC AGCCCCAAGC CTTCCTGCTG 
CTACTACAGA GCCTGGAGAC CCCCGAAAAC AAGGCCCTTC AAATGACTGG GCTGAGAATA 
CTGAGCTGTC TCCTGGATGT CTCTGTGGAC TCAAGTCTTC CTTGGCACAA AGTGGCCTCT 
TCCTGTTTGG CCAGCCTGAG AACACTCAGC AAAGACCATG AGGTGGTCCA AGGGTTAATC 
TGTTTCCTGC ATCGCCTGAT CACACTACAC AAGGACTGCG CAGTGGTTCT GTACCAGCTC 
GGAGCTCAAG AGATTCTCAC CAAGGCCCTG GACAAGCACA AGGAACAACT GTTTCTGGGC 
TCTGAGCTCC AAGATCTAGT GGGAGAGTAT GAGAAGCACG CCCAGCTTTA CAGCAACCTC 
ACCAAAAGCA TCTTGGCCGG CTGCATCCAG ATGGTGCTCG GCCAGATTGA AGAGCACCGA 
AGAACCCACC GGCCCATTAA CATCCCTTTC TTTGACGTCT TCCTCAGGCA TCTTTGCCAG 
GGCTCTGATG TGGAGATGAA GGACAAGTGT TGGGAGAAGA TGGAGGTGTC GTCTAATTCT 
CTCGGGGCCA ACAAACTGAC TGACCGAAAC CCCAAGACTT ACTGGGAATC CAGCGGCACT 
ACAGGGTCTC ACTACATCAC ATTACACATG CATCAAGGGG CCATTGTCAG ACAGCTGACT 
CTCCTGGTGT CTAAGGAAGA CTCAAGCTAC ATGCCGGCCC GGGTGGTGGT GCTGGGGGGA 
GACCATGCCC GCTCTGTCAG CACAAAGCTC AGCACTGTAA ATGTCATGCC TTCTGACACG 
CGGGTGCTTC TTCTGGAGAA CATGACCCGC TTCTGGCCCA TCCTACAGAT CCATATCAAG 
CGCTGCCAGC AGGGTGGCAT TGACACCCGT GTGCGAGGGG TGGAGGTTCT GGGTCCCAAA 
CCCACTTTCT GGCCACTGTT CCGTGAGCAG CTGTGTCGCC GCACTCACCT TTTCTACACC 
ATACGAGCCC AAGCCTGGAG TCAGGATATT GCTCAGGACC GGAAGCGCCT GCTGCAGCTC 
TGCCCTAGAC TGAACGGGGC TTTGCGCCAT GAGCAGAGTT TTGCAGATCG ATTTCTTCCT 
GATGATGAGG CTGCCCAGGC ACTGGGAAGG ACCTGCTGGG AAGCCTTGGT CAGCCCCTTG 
GTGCAGAGCA TCACTGCCCC TGATGGCAGT GGGGTAAGCC CCCTGGCCTG GCTGCTGAGC 
CAGTATCTGG AGCACAGAGA GAAGGTCCAG CACAAACAGG AACAAGCAGA TGCTTTTACC 
TCACGTGTCC GATGTCTCAC TCACCTGCTG GTGCATGTGG AGCCTCCCTC GGGTCTCCAG 
TCCAAGTTAC TCACCTGGCC TAATGGCAAG AATAGTAAGA GTCGGGAGCT GAACTCGGGG 
GCTGGACTGG GACCTCCTGG TGGCAGCCTG AGGGGTATCA CCCAGTGTTG GAGGGGAGTG 
GTACAAGAGC AGGTGAGCAG GTTTCTGACT GCGGCCTGGC AGGCCCCGGA CCTGGTGCCC 
AGCTACTGCG ACATCTACGA ACGATTTTAT TATGTGTTTG TTAAATTGGA CCGACTTGAA 
GGAGGAAGGT CCCTGAGAGA CATTTGTCCC CACAGCCAGT GGAGGAGCTG GTGGCTGAGG 
AGCCAGTCCC TGAAGTGTCT GTGCTGGTCC TGTCTCCCCG CTGCTGGCCC GTGTCCCCCC 
TCTGCTACCT GCACGAGCCT ACCAAGTACT TCCCCCCGGC TCTCAGCACC AGCCTCACCC 
GGTTTTCCAA CTTCTACAGT CAGAGAGGTG GCCTTGGAGA CGCTGCTGCA GACCTCAGAC 
CTGCCCTCAG AGCTTCTGAT GCAAGCACTT AAGCCCCTCA CTTCAGGGCT TGGCCCCCTG 
ACCCTCCAGG AAGGCCAGGG TCTCCCACCC GGGGGTGTGC TGCGGCTTCC GGAGGTGGAG 
GAGGAGCCTG GGGGGGAGGC CCTGTGGGGC GGGGCCGCGG GACCAGGTAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100932280 ( XM_023503012.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023358780.1 Sarcophilus harrisii cullin-7-like (LOC100932280), mRNA.

Price & Availability↑

CloneID	OSa154826
Clone ID Related Accession (Same CDS sequence)	XM_023503012.1
Accession Version	XM_023503012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	cullin-7-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838897.1) and transcript sequence (GEDN01156573.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 11% of CDS bases assembly gap :: added 506 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGTTCGC CCCATCTGCT CCTTTCACCC CCACCTAAGA CCTTCGGCTT TCCAGCGGTT 
AAACGCGGTC TGGACGAAGT GCTCTTCCAG TCCGGGATCA TTCCGAGTCC TCTCCTCCCC 
GTGGCAGAGC TATCCCAGAT CTGGGGATCT GACCCTCGCG CCCAACCCCG GTCCAGTCAG 
TACACACCTC CTCCTGGTCC GGGAGCGTCT CGGCGCCGGA GTGGCATTAC GCAGGTCTGG 
TCCGGGCGAG TGTCTTCCCC TGCGACCCTA CTCCCTCCTG TGGGCTTCTG CATCCGCCAC 
ACCCTGCTTC CCTCCTTCCC TGCCGCGACT AAACTTCCTC CACTCTTCAC ACTCCAGGTG 
CTGTGGCAGT CGACAGGCCG CACATACTGG GTGCACTGGC ACATGGTGGA GATTCTGGGT 
CCTGTAACTG AGGACAAAGT TACTATTGAG CCAGAGAAAG GAACTGGGGG CAGCAGGTGC 
CTGGCCCCAA GTATAGCACG TCCATTCTTA GAGTGGAAAC CTATTGTGGG ACTGTATCCT 
CTGCCGTACC TTAAACCTGA GGCTCAGAGC AACACAGAGT CTGGGTGCCT GAGCCAGGCC 
GAATGGTGGG AACTTCTTTT CTTCATCAAA AAGCTGGATG GGCCTGAGCA ACAGGAGTTT 
CTCCGGCTCC TCCAGGAGAA CCCGGATGGG GAGCCTGTGG AAGAAGAAGC CCTTGGTGAA 
CTCAAAGTAT CTGTGGAGTT GGCAGAGAAA CTGGTCCTGG CACTCAGCAG GCAGGTTCAA 
GGCAGCATCC ACAATGACCT GCTTAGTTCG ATTGTTTACA ACAAATTCAG GTTACAGCAG 
GTGGAGGATT TGGCCAACCC TACTCCTCCA GAGCCTGGTC AGGACACCTG CAGCCCTGAG 
GCCCCATTTC CAGACACCAA ATCAGAAGTG GAGGTGAAAG GGCAGATTAA ATCACTAGGG 
GTGGAGGAGT CATCAACAGC ACCGGAAGTT GAGCTGCTGT TTCGGAGCCT CGTGGGGGCT 
GCAGAGCCCT CTGGACAGCT GCTCCTGGAG CTGGAGCTAA GCATATGCCA GGAGGGCACT 
GGGGAGGGTC AAGTGAGCCA GCTCCTGGGG CAGCTCCAAC ACCAGCCCCA AGCCTTCCTG 
CTGCTACTAC AGAGCCTGGA GACCCCCGAA AACAAGGCCC TTCAAATGAC TGGGCTGAGA 
ATACTGAGCT GTCTCCTGGA TGTCTCTGTG GACTCAAGTC TTCCTTGGCA CAAAGTGGCC 
TCTTCCTGTT TGGCCAGCCT GAGAACACTC AGCAAAGACC ATGAGGTGGT CCAAGGGTTA 
ATCTGTTTCC TGCATCGCCT GATCACACTA CACAAGGACT GCGCAGTGGT TCTGTACCAG 
CTCGGAGCTC AAGAGATTCT CACCAAGGCC CTGGACAAGC ACAAGGAACA ACTGTTTCTG 
GGCTCTGAGC TCCAAGATCT AGTGGGAGAG TATGAGAAGC ACGCCCAGCT TTACAGCAAC 
CTCACCAAAA GCATCTTGGC CGGCTGCATC CAGATGGTGC TCGGCCAGAT TGAAGAGCAC 
CGAAGAACCC ACCGGCCCAT TAACATCCCT TTCTTTGACG TCTTCCTCAG GCATCTTTGC 
CAGGGCTCTG ATGTGGAGAT GAAGGACAAG TGTTGGGAGA AGATGGAGGT GTCGTCTAAT 
TCTCTCGGGG CCAACAAACT GACTGACCGA AACCCCAAGA CTTACTGGGA ATCCAGCGGC 
ACTACAGGGT CTCACTACAT CACATTACAC ATGCATCAAG GGGCCATTGT CAGACAGCTG 
ACTCTCCTGG TGTCTAAGGA AGACTCAAGC TACATGCCGG CCCGGGTGGT GGTGCTGGGG 
GGAGACCATG CCCGCTCTGT CAGCACAAAG CTCAGCACTG TAAATGTCAT GCCTTCTGAC 
ACGCGGGTGC TTCTTCTGGA GAACATGACC CGCTTCTGGC CCATCCTACA GATCCATATC 
AAGCGCTGCC AGCAGGGTGG CATTGACACC CGTGTGCGAG GGGTGGAGGT TCTGGGTCCC 
AAACCCACTT TCTGGCCACT GTTCCGTGAG CAGCTGTGTC GCCGCACTCA CCTTTTCTAC 
ACCATACGAG CCCAAGCCTG GAGTCAGGAT ATTGCTCAGG ACCGGAAGCG CCTGCTGCAG 
CTCTGCCCTA GACTGAACGG GGCTTTGCGC CATGAGCAGA GTTTTGCAGA TCGATTTCTT 
CCTGATGATG AGGCTGCCCA GGCACTGGGA AGGACCTGCT GGGAAGCCTT GGTCAGCCCC 
TTGGTGCAGA GCATCACTGC CCCTGATGGC AGTGGGGTAA GCCCCCTGGC CTGGCTGCTG 
AGCCAGTATC TGGAGCACAG AGAGAAGGTC CAGCACAAAC AGGAACAAGC AGATGCTTTT 
ACCTCACGTG TCCGATGTCT CACTCACCTG CTGGTGCATG TGGAGCCTCC CTCGGGTCTC 
CAGTCCAAGT TACTCACCTG GCCTAATGGC AAGAATAGTA AGAGTCGGGA GCTGAACTCG 
GGGGCTGGAC TGGGACCTCC TGGTGGCAGC CTGAGGGGTA TCACCCAGTG TTGGAGGGGA 
GTGGTACAAG AGCAGGTGAG CAGGTTTCTG ACTGCGGCCT GGCAGGCCCC GGACCTGGTG 
CCCAGCTACT GCGACATCTA CGAACGGCTC CAGAGCGCTG GGGCGGAGCT CTTTGGGCCG 
CGGGCAGCCT TCACGCTGGC CCTGCGCCAG GGCTTCTCTG GGGCTCTGCT GCAGCTCTCC 
TTCCTCACCG CTGCCCACGT GAGCGAGCAG TTCGCCCGGC ACATCGACCA GCAGATCCAG 
AGGAGCCGTG TAGGTGGCCC TCAGCGGGAA GAGATGCTGG GCCAGCTGCA GCGGAGCCTG 
GAGCCCATGA TGGTCCTGTC TGGCCTAGAG CTGGCCACCA CCTTTGAGCA CTTTTACCAG 
TACTATCTGG CAGACCGCCT CCTGAGCCTG GGCCCGAGCT GGCTGGAGCG GGCCGTGCTG 
GAGCAGATTG GGCTCTGCTT CCCCAACCGA CTACCCCAGC AGATGCTGAG CAGCCTGAGC 
ACGTCGGAGG AGCTGCAGCA CCAGTTCCAC CTCTTCCAGC TTGAGCAGAT CGACCAGCAA 
TTCCTGGAAC AGGAAGAGGA TGAAGAGCAA GGGCTGGAGG CCTATCCTCA GGAAGAGCCA 
GTGGAGGAGC TGGTGGCTGA GGAGCCAGTC CCTGAAGTGT CTGTGCTGGT CCTGTCTCCC 
CGCTGCTGGC CCGTGTCCCC CCTCTGCTAC CTGCACGAGC CTACCAAGTA CTTCCCCCCG 
GCTCTCAGCA CCAGCCTCAC CCGGTTTTCC AACTTCTACA GTCAGAGTCA GAATCGGCCA 
GTCTTGGGGG CACAGCGCCT ACTGCAGTGG TCATGGTTGG GCAGGGCCGA ACTGTCATTT 
GGAGCTCAGA CTCTTCATGT CTCTACTTTA CAGATGTGGC TTCTTCTGCA TTTTAACCAA 
ATGGAGGAGG TGGCCTTGGA GACGCTGCTG CAGACCTCAG ACCTGCCCTC AGAGCTTCTG 
ATGCAAGCAC TTAAGCCCCT CACTTCAGGG CTTGGCCCCC TGACCCTCCA GGAAGGCCAG 
GGTCTCCCAC CCGGGGGTGT GCTGCGGCTT CCGGAGGTGG AGGAGGAGCC TGGGGGGGAG 
GCCCTGTGGG GCGGGGCCGC GGGACCAGGT ATCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023358780.1
CDS	1..3699
Translation	MGSPHLLLSPPPKTFGFPAVKRGLDEVLFQSGIIPSPLLPVAELSQIWGSDPRAQPRSSQYTPPPGPGASRRRSGITQVWSGRVSSPATLLPPVGFCIRHTLLPSFPAATKLPPLFTLQVLWQSTGRTYWVHWHMVEILGPVTEDKVTIEPEKGTGGSRCLAPSIARPFLEWKPIVGLYPLPYLKPEAQSNTESGCLSQAEWWELLFFIKKLDGPEQQEFLRLLQENPDGEPVEEEALGELKVSVELAEKLVLALSRQVQGSIHNDLLSSIVYNKFRLQQVEDLANPTPPEPGQDTCSPEAPFPDTKSEVEVKGQIKSLGVEESSTAPEVELLFRSLVGAAEPSGQLLLELELSICQEGTGEGQVSQLLGQLQHQPQAFLLLLQSLETPENKALQMTGLRILSCLLDVSVDSSLPWHKVASSCLASLRTLSKDHEVVQGLICFLHRLITLHKDCAVVLYQLGAQEILTKALDKHKEQLFLGSELQDLVGEYEKHAQLYSNLTKSILAGCIQMVLGQIEEHRRTHRPINIPFFDVFLRHLCQGSDVEMKDKCWEKMEVSSNSLGANKLTDRNPKTYWESSGTTGSHYITLHMHQGAIVRQLTLLVSKEDSSYMPARVVVLGGDHARSVSTKLSTVNVMPSDTRVLLLENMTRFWPILQIHIKRCQQGGIDTRVRGVEVLGPKPTFWPLFREQLCRRTHLFYTIRAQAWSQDIAQDRKRLLQLCPRLNGALRHEQSFADRFLPDDEAAQALGRTCWEALVSPLVQSITAPDGSGVSPLAWLLSQYLEHREKVQHKQEQADAFTSRVRCLTHLLVHVEPPSGLQSKLLTWPNGKNSKSRELNSGAGLGPPGGSLRGITQCWRGVVQEQVSRFLTAAWQAPDLVPSYCDIYERLQSAGAELFGPRAAFTLALRQGFSGALLQLSFLTAAHVSEQFARHIDQQIQRSRVGGPQREEMLGQLQRSLEPMMVLSGLELATTFEHFYQYYLADRLLSLGPSWLERAVLEQIGLCFPNRLPQQMLSSLSTSEELQHQFHLFQLEQIDQQFLEQEEDEEQGLEAYPQEEPVEELVAEEPVPEVSVLVLSPRCWPVSPLCYLHEPTKYFPPALSTSLTRFSNFYSQSQNRPVLGAQRLLQWSWLGRAELSFGAQTLHVSTLQMWLLLHFNQMEEVALETLLQTSDLPSELLMQALKPLTSGLGPLTLQEGQGLPPGGVLRLPEVEEEPGGEALWGGAAGPGIS

Target ORF information:

RefSeq Version	XM_023503012.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii cullin-7-like (LOC100932280), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023503012.1

ORF Insert Sequence:

ATGGGTTCGC CCCATCTGCT CCTTTCACCC CCACCTAAGA CCTTCGGCTT TCCAGCGGTT 
AAACGCGGTC TGGACGAAGT GCTCTTCCAG TCCGGGATCA TTCCGAGTCC TCTCCTCCCC 
GTGGCAGAGC TATCCCAGAT CTGGGGATCT GACCCTCGCG CCCAACCCCG GTCCAGTCAG 
TACACACCTC CTCCTGGTCC GGGAGCGTCT CGGCGCCGGA GTGGCATTAC GCAGGTCTGG 
TCCGGGCGAG TGTCTTCCCC TGCGACCCTA CTCCCTCCTG TGGGCTTCTG CATCCGCCAC 
ACCCTGCTTC CCTCCTTCCC TGCCGCGACT AAACTTCCTC CACTCTTCAC ACTCCAGGTG 
CTGTGGCAGT CGACAGGCCG CACATACTGG GTGCACTGGC ACATGGTGGA GATTCTGGGT 
CCTGTAACTG AGGACAAAGT TACTATTGAG CCAGAGAAAG GAACTGGGGG CAGCAGGTGC 
CTGGCCCCAA GTATAGCACG TCCATTCTTA GAGTGGAAAC CTATTGTGGG ACTGTATCCT 
CTGCCGTACC TTAAACCTGA GGCTCAGAGC AACACAGAGT CTGGGTGCCT GAGCCAGGCC 
GAATGGTGGG AACTTCTTTT CTTCATCAAA AAGCTGGATG GGCCTGAGCA ACAGGAGTTT 
CTCCGGCTCC TCCAGGAGAA CCCGGATGGG GAGCCTGTGG AAGAAGAAGC CCTTGGTGAA 
CTCAAAGTAT CTGTGGAGTT GGCAGAGAAA CTGGTCCTGG CACTCAGCAG GCAGGTTCAA 
GGCAGCATCC ACAATGACCT GCTTAGTTCG ATTGTTTACA ACAAATTCAG GTTACAGCAG 
GTGGAGGATT TGGCCAACCC TACTCCTCCA GAGCCTGGTC AGGACACCTG CAGCCCTGAG 
GCCCCATTTC CAGACACCAA ATCAGAAGTG GAGGTGAAAG GGCAGATTAA ATCACTAGGG 
GTGGAGGAGT CATCAACAGC ACCGGAAGTT GAGCTGCTGT TTCGGAGCCT CGTGGGGGCT 
GCAGAGCCCT CTGGACAGCT GCTCCTGGAG CTGGAGCTAA GCATATGCCA GGAGGGCACT 
GGGGAGGGTC AAGTGAGCCA GCTCCTGGGG CAGCTCCAAC ACCAGCCCCA AGCCTTCCTG 
CTGCTACTAC AGAGCCTGGA GACCCCCGAA AACAAGGCCC TTCAAATGAC TGGGCTGAGA 
ATACTGAGCT GTCTCCTGGA TGTCTCTGTG GACTCAAGTC TTCCTTGGCA CAAAGTGGCC 
TCTTCCTGTT TGGCCAGCCT GAGAACACTC AGCAAAGACC ATGAGGTGGT CCAAGGGTTA 
ATCTGTTTCC TGCATCGCCT GATCACACTA CACAAGGACT GCGCAGTGGT TCTGTACCAG 
CTCGGAGCTC AAGAGATTCT CACCAAGGCC CTGGACAAGC ACAAGGAACA ACTGTTTCTG 
GGCTCTGAGC TCCAAGATCT AGTGGGAGAG TATGAGAAGC ACGCCCAGCT TTACAGCAAC 
CTCACCAAAA GCATCTTGGC CGGCTGCATC CAGATGGTGC TCGGCCAGAT TGAAGAGCAC 
CGAAGAACCC ACCGGCCCAT TAACATCCCT TTCTTTGACG TCTTCCTCAG GCATCTTTGC 
CAGGGCTCTG ATGTGGAGAT GAAGGACAAG TGTTGGGAGA AGATGGAGGT GTCGTCTAAT 
TCTCTCGGGG CCAACAAACT GACTGACCGA AACCCCAAGA CTTACTGGGA ATCCAGCGGC 
ACTACAGGGT CTCACTACAT CACATTACAC ATGCATCAAG GGGCCATTGT CAGACAGCTG 
ACTCTCCTGG TGTCTAAGGA AGACTCAAGC TACATGCCGG CCCGGGTGGT GGTGCTGGGG 
GGAGACCATG CCCGCTCTGT CAGCACAAAG CTCAGCACTG TAAATGTCAT GCCTTCTGAC 
ACGCGGGTGC TTCTTCTGGA GAACATGACC CGCTTCTGGC CCATCCTACA GATCCATATC 
AAGCGCTGCC AGCAGGGTGG CATTGACACC CGTGTGCGAG GGGTGGAGGT TCTGGGTCCC 
AAACCCACTT TCTGGCCACT GTTCCGTGAG CAGCTGTGTC GCCGCACTCA CCTTTTCTAC 
ACCATACGAG CCCAAGCCTG GAGTCAGGAT ATTGCTCAGG ACCGGAAGCG CCTGCTGCAG 
CTCTGCCCTA GACTGAACGG GGCTTTGCGC CATGAGCAGA GTTTTGCAGA TCGATTTCTT 
CCTGATGATG AGGCTGCCCA GGCACTGGGA AGGACCTGCT GGGAAGCCTT GGTCAGCCCC 
TTGGTGCAGA GCATCACTGC CCCTGATGGC AGTGGGGTAA GCCCCCTGGC CTGGCTGCTG 
AGCCAGTATC TGGAGCACAG AGAGAAGGTC CAGCACAAAC AGGAACAAGC AGATGCTTTT 
ACCTCACGTG TCCGATGTCT CACTCACCTG CTGGTGCATG TGGAGCCTCC CTCGGGTCTC 
CAGTCCAAGT TACTCACCTG GCCTAATGGC AAGAATAGTA AGAGTCGGGA GCTGAACTCG 
GGGGCTGGAC TGGGACCTCC TGGTGGCAGC CTGAGGGGTA TCACCCAGTG TTGGAGGGGA 
GTGGTACAAG AGCAGGTGAG CAGGTTTCTG ACTGCGGCCT GGCAGGCCCC GGACCTGGTG 
CCCAGCTACT GCGACATCTA CGAACGGCTC CAGAGCGCTG GGGCGGAGCT CTTTGGGCCG 
CGGGCAGCCT TCACGCTGGC CCTGCGCCAG GGCTTCTCTG GGGCTCTGCT GCAGCTCTCC 
TTCCTCACCG CTGCCCACGT GAGCGAGCAG TTCGCCCGGC ACATCGACCA GCAGATCCAG 
AGGAGCCGTG TAGGTGGCCC TCAGCGGGAA GAGATGCTGG GCCAGCTGCA GCGGAGCCTG 
GAGCCCATGA TGGTCCTGTC TGGCCTAGAG CTGGCCACCA CCTTTGAGCA CTTTTACCAG 
TACTATCTGG CAGACCGCCT CCTGAGCCTG GGCCCGAGCT GGCTGGAGCG GGCCGTGCTG 
GAGCAGATTG GGCTCTGCTT CCCCAACCGA CTACCCCAGC AGATGCTGAG CAGCCTGAGC 
ACGTCGGAGG AGCTGCAGCA CCAGTTCCAC CTCTTCCAGC TTGAGCAGAT CGACCAGCAA 
TTCCTGGAAC AGGAAGAGGA TGAAGAGCAA GGGCTGGAGG CCTATCCTCA GGAAGAGCCA 
GTGGAGGAGC TGGTGGCTGA GGAGCCAGTC CCTGAAGTGT CTGTGCTGGT CCTGTCTCCC 
CGCTGCTGGC CCGTGTCCCC CCTCTGCTAC CTGCACGAGC CTACCAAGTA CTTCCCCCCG 
GCTCTCAGCA CCAGCCTCAC CCGGTTTTCC AACTTCTACA GTCAGAGTCA GAATCGGCCA 
GTCTTGGGGG CACAGCGCCT ACTGCAGTGG TCATGGTTGG GCAGGGCCGA ACTGTCATTT 
GGAGCTCAGA CTCTTCATGT CTCTACTTTA CAGATGTGGC TTCTTCTGCA TTTTAACCAA 
ATGGAGGAGG TGGCCTTGGA GACGCTGCTG CAGACCTCAG ACCTGCCCTC AGAGCTTCTG 
ATGCAAGCAC TTAAGCCCCT CACTTCAGGG CTTGGCCCCC TGACCCTCCA GGAAGGCCAG 
GGTCTCCCAC CCGGGGGTGT GCTGCGGCTT CCGGAGGTGG AGGAGGAGCC TGGGGGGGAG 
GCCCTGTGGG GCGGGGCCGC GGGACCAGGT ATCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100932280 ( XM_031968620.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031824480.1 Sarcophilus harrisii cullin 7 (CUL7), mRNA.

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CloneID	OSa235352
Clone ID Related Accession (Same CDS sequence)	XM_031968620.1
Accession Version	XM_031968620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	cullin-7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGTGGGAG AACGCAGAAG CAGGGACCTG CTGGTGCCCC TGGGCCCACG GCTGCAGGCG 
TACCCCGAGG AACTGCTGCG GCAGCGCCAG GGCCACGATG GGCATCCAGA GTACTTGATC 
CGTTGGACCG TCCTTCAGCG CGGGAAGGAG GGCGGGGTGG GCAGCGGCAG CTCCATAGAG 
GGCAAGGCGG AGCACATCCT CATGTGGCTC TCTGCCCCCG AGGTCTACGC CAACTGCCCC 
ATGCTCCTTG GCGAGCGCGC GCCAGCCAAG GGGCCCCCGC ACGAACCCGT GGGCGGCGTG 
GGCGCCTTTC CCCGGGATGC CGGGGGGCTG GACGAAGCCT CGCTGGGGGA GATGGCCGCA 
GACGTGCGAG GACTGGTGCG TCGAGCCGCC CGGCAGCTGG ATGGGGGGGG GGGCTCGAGT 
CCCGCGGCCT CGGTGCTACA CACCATCCAC GTGCTCAGCG CCTACGCCAG CATCGGACCC 
CTGGCTGGCG TCTTCCGGGA GACGGGAGCC TTGGACCTGC TCATGCACAT GCTGGGCAAC 
CCCGAGTCCC AGATCCGCAG GAGTGCCGGG AAGATGCTGC AGGCCTTGGC AGCCCACGAC 
GCTGGGAGCA GGGCCCATGT GCTCCTGTCA CTGAGCCAGC AGGATGGCAT CGAGCAGCAC 
ATGGACTTTG ACAGCCGCTC CACTCTGTTG GAGCTGTTTG CAGAGACCAC ATCCTCCGAA 
GAGCACTGCA TGGCCTTTGA AGGGATCCAC CTGCCCCAGA TCCCCGGAAA GCTGCTCTTC 
TCCTTAGTGA AGCGTTACTT GTGTGTCACG TCACTCCTGG ACCAGCTAAG TAGCAGCGTG 
GAGCCAGGGT CTGGGGAGTG GGGAAACCGG TGCTCCCCTA ACAAGTCCTC CAGTGTGGAG 
AAGAGCCGTG GCCAGAGGGA ACTGGAGTTT AGCATGGCCA TGGGCAACCT GATTTCAGAA 
TTGGTTCGGG GCATGGGATG GGACCGGGGC CCGGGGAGGC AGCCTGCGTC TCCCCCTCGG 
CCCGCCCGGT CTATCTTTCA GCCCAGGTCC TCTGTGCCGA GCACCATCCT CCCCACCCTG 
CTTCCCCCTG CTTCCCCCAG GAGGCCGAGC CGGGCCTTCA GGCCTCGGTC CGAGTTCTCG 
AGCCTGAGTG GCTATGCGGA CTACGTCCAG GAGACCCTGC AGCCAGGGAT GCGCGTTCGA 
ATGCTGGAGG ATTATGAGGA GATCAGTGCC GGGGACCAAG GCGAGTTTCG GCAGAGCAAC 
AATGGAATGC CCCCTGTGCA GGTGCTGTGG CAGTCGACAG GCCGCACATA CTGGGTGCAC 
TGGCACATGG TGGAGATTCT GGGTCCTGTA ACTGAGGACA AAGTTACTAT TGAGCCAGAG 
AAAGGAACTG GGGGCAGCAG GTGCCTGGCC CCAAGTATAG CACGTCCATT CTTAGAGTGG 
AAACCTATTG TGGGACTGTA TCCTCTGCCG TACCTTAAAC CTGAGGCTCA GAGCAACACA 
GAGTCTGGGT GCCTGAGCCA GGCCGAATGG TGGGAACTTC TTTTCTTCAT CAAAAAGCTG 
GATGGGCCTG AGCAACAGGA GTTTCTCCGG CTCCTCCAGG AGAACCCGGA TGGGGAGCCT 
GTGGAAGAAG AAGCCCTTGG TGAACTCAAA GTATCTGTGG AGTTGGCAGA GAAACTGGTC 
CTGGCACTCA GCAGGCAGGT TCAAGGCAGC ATCCACAATG ACCTGCTTAG TTCGATTGTT 
TACAACAAAT TCAGGTTACA GCAGGTGGAG GATTTGGCCA ACCCTACTCC TCCAGAGCCT 
GGTCAGGACA CCTGCAGCCC TGAGGCCCCA TTTCCAGACA CCAAATCAGA AGTGGAGGTG 
AAAGGGCAGA TTAAATCACT AGGGGTGGAG GAGTCATCAA CAGCACCGGA AGTTGAGCTG 
CTGTTTCGGA GCCTCGTGGG GGCTGCAGAG CCCTCTGGAC AGCTGCTCCT GGAGCTGGAG 
CTAAGCATAT GCCAGGAGGG CACTGGGGAG GGTCAAGTGA GCCAGCTCCT GGGGCAGCTC 
CAACACCAGC CCCAAGCCTT CCTGCTGCTA CTACAGAGCC TGGAGACCCC CGAAAACAAG 
GCCCTTCAAA TGACTGGGCT GAGAATACTG AGCTGTCTCC TGGATGTCTC TGTGGACTCA 
AGTCTTCCTT GGCACAAAGT GGCCTCTTCC TGTTTGGCCA GCCTGAGAAC ACTCAGCAAA 
GACCATGAGG TGGTCCAAGG GTTAATCTGT TTCCTGCATC GCCTGATCAC ACTACACAAG 
GACTGCGCAG TGGTTCTGTA CCAGCTCGGA GCTCAAGAGA TTCTCACCAA GGCCCTGGAC 
AAGCACAAGG AACAACTGTT TCTGGGCTCT GAGCTCCAAG ATCTAGTGGG AGAGTATGAG 
AAGCACGCCC AGCTTTACAG CAACCTCACC AAAAGCATCT TGGCCGGCTG CATCCAGATG 
GTGCTCGGCC AGATTGAAGA GCACCGAAGA ACCCACCGGC CCATTAACAT CCCTTTCTTT 
GACGTCTTCC TCAGGCATCT TTGCCAGGGC TCTGATGTGG AGATGAAGGA CAAGTGTTGG 
GAGAAGATGG AGGTGTCGTC TAATTCTCTC GGGGCCAACA AACTGACTGA CCGAAACCCC 
AAGACTTACT GGGAATCCAG CGGCACTACA GGGTCTCACT ACATCACATT ACACATGCAT 
CAAGGGGCCA TTGTCAGACA GCTGACTCTC CTGGTGTCTA AGGAAGACTC AAGCTACATG 
CCGGCCCGGG TGGTGGTGCT GGGGGGAGAC CATGCCCGCT CTGTCAGCAC AAAGCTCAGC 
ACTGTAAATG TCATGCCTTC TGACACGCGG GTGCTTCTTC TGGAGAACAT GACCCGCTTC 
TGGCCCATCC TACAGATCCA TATCAAGCGC TGCCAGCAGG GTGGCATTGA CACCCGTGTG 
CGAGGGGTGG AGGTTCTGGG TCCCAAACCC ACTTTCTGGC CACTGTTCCG TGAGCAGCTG 
TGTCGCCGCA CTCACCTTTT CTACACCATA CGAGCCCAAG CCTGGAGTCA GGATATTGCT 
CAGGACCGGA AGCGCCTGCT GCAGCTCTGC CCTAGACTGA ACGGGGCTTT GCGCCATGAG 
CAGAGTTTTG CAGATCGATT TCTTCCTGAT GATGAGGCTG CCCAGGCACT GGGAAGGACC 
TGCTGGGAAG CCTTGGTCAG CCCCTTGGTG CAGAGCATCA CTGCCCCTGA TGGCAGTGGG 
GTAAGCCCCC TGGCCTGGCT GCTGAGCCAG TATCTGGAGC ACAGAGAGAA GGTCCAGCAC 
AAACAGGAAC AAGCAGATGC TTTTACCTCA CGTGTCCGAT GTCTCACTCA CCTGCTGGTG 
CATGTGGAGC CTCCCTCGGG TCTCCAGTCC AAGTTACTCA CCTGGCCTAA TGGCAAGAAT 
AGTAAGAGTC GGGAGCTGAA CTCGGGGTTG GGATTGGGGA CCTGGGGGGT CCCGGCCCGA 
TGGACCGAAG GGAGCCTTGG GGGGGGGCAG CCTTCCGCTG GCCCTGCGCC AGGGCTTTCT 
CTGGGGTTTC TCAAGCCTTC CTTCCTCACC GGCCCACGTG AGGAGATGGG CCGGGCAATG 
CCACAGATCC AGAGGAGGTG GTGGCCCCTA GGGGAAGAGA TGGGCCAGCT CAGCGGAGCC 
TGGAGCCATG ATGGTCCTTT CGGCCTAGAG TGGAAGAAGG TCCCTGAGAG ACATTTGTCC 
CCACAGCCAG TGGAGGAGCT GGTGGCTGAG GAGCCAGTCC CTGAAGTGTC TGTGCTGGTC 
CTGTCTCCCC GCTGCTGGCC CGTGTCCCCC CTCTGCTACC TGCACGAGCC TACCAAGTAC 
TTCCCCCCGG CTCTCAGCAC CAGCCTCACC CGGTTTTCCA ACTTCTACAG TCAGAGTCAG 
AATCGGCCAG TCTTGGGGGC ACAGCGCCTA CTGCAGTGGT CATGGTTGGG CAGGGCCGAA 
CTGTCATTTG GAGCTCAGAC TCTTCATGTC TCTACTTTAC AGATGTGGCT TCTTCTGCAT 
TTTAACCAAA TGGAGGAGGT GGCCTTGGAG ACGCTGCTGC AGACCTCAGA CCTGCCCTCA 
GAGCTTCTGA TGCAAGCACT TAAGCCCCTC ACTTCAGGGC TTGGCCCCCT GACCCTCCAG 
GAAGGCCAGG GTCTCCCACC CGGGGGTGTG CTGCGGCTTC CGGAGGTGGA GGAGGAGCCT 
GGGGGGGAGG CCCTGTGGCT GCTCCCCCCC CAGACATACC TGAGTGTGGA GGAGGATGAA 
TGCCGAACCC TGGAGAGGAA GAGGAACCTC CTGAGCTGCC TCCTTGTCCG AATCCTCAAA 
GCGCACGGCG AGAAAGGCCT GCACATTGAC CAGCTTGTCT GTCTGGTGCT GGAGGCCTGG 
CAGAAGGGCC AGCCTTCCCC CAGAGACCCC GGCAACAGCA CGGGCGGTGA GGACGAGTGT 
AATGGGGCCG ACGTCCTCTC CTGCATCCTC CACCTCCTGG GCCAGGGCTA TGTTCGGAGG 
CAGGAGGACC GGCCCCAAGT CCTGGCATAT GCAGCCCCGG AGCCCGAGGG GCCTGCCCTT 
GCACCCCCCA ACCTGGTTGC CTTGGGGCCT GCCCCACCCT CTACCTTCCA CACCCTGGAG 
ATCCGAACCC GAACCACCGC CAGCCCCTCC CCTCCTGCTC GGACTTTTTC CACCTTCCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031824480.1
CDS	593..5275
Translation	MVGERRSRDLLVPLGPRLQAYPEELLRQRQGHDGHPEYLIRWTVLQRGKEGGVGSGSSIEGKAEHILMWLSAPEVYANCPMLLGERAPAKGPPHEPVGGVGAFPRDAGGLDEASLGEMAADVRGLVRRAARQLDGGGGSSPAASVLHTIHVLSAYASIGPLAGVFRETGALDLLMHMLGNPESQIRRSAGKMLQALAAHDAGSRAHVLLSLSQQDGIEQHMDFDSRSTLLELFAETTSSEEHCMAFEGIHLPQIPGKLLFSLVKRYLCVTSLLDQLSSSVEPGSGEWGNRCSPNKSSSVEKSRGQRELEFSMAMGNLISELVRGMGWDRGPGRQPASPPRPARSIFQPRSSVPSTILPTLLPPASPRRPSRAFRPRSEFSSLSGYADYVQETLQPGMRVRMLEDYEEISAGDQGEFRQSNNGMPPVQVLWQSTGRTYWVHWHMVEILGPVTEDKVTIEPEKGTGGSRCLAPSIARPFLEWKPIVGLYPLPYLKPEAQSNTESGCLSQAEWWELLFFIKKLDGPEQQEFLRLLQENPDGEPVEEEALGELKVSVELAEKLVLALSRQVQGSIHNDLLSSIVYNKFRLQQVEDLANPTPPEPGQDTCSPEAPFPDTKSEVEVKGQIKSLGVEESSTAPEVELLFRSLVGAAEPSGQLLLELELSICQEGTGEGQVSQLLGQLQHQPQAFLLLLQSLETPENKALQMTGLRILSCLLDVSVDSSLPWHKVASSCLASLRTLSKDHEVVQGLICFLHRLITLHKDCAVVLYQLGAQEILTKALDKHKEQLFLGSELQDLVGEYEKHAQLYSNLTKSILAGCIQMVLGQIEEHRRTHRPINIPFFDVFLRHLCQGSDVEMKDKCWEKMEVSSNSLGANKLTDRNPKTYWESSGTTGSHYITLHMHQGAIVRQLTLLVSKEDSSYMPARVVVLGGDHARSVSTKLSTVNVMPSDTRVLLLENMTRFWPILQIHIKRCQQGGIDTRVRGVEVLGPKPTFWPLFREQLCRRTHLFYTIRAQAWSQDIAQDRKRLLQLCPRLNGALRHEQSFADRFLPDDEAAQALGRTCWEALVSPLVQSITAPDGSGVSPLAWLLSQYLEHREKVQHKQEQADAFTSRVRCLTHLLVHVEPPSGLQSKLLTWPNGKNSKSRELNSGLGLGTWGVPARWTEGSLGGGQPSAGPAPGLSLGFLKPSFLTGPREEMGRAMPQIQRRWWPLGEEMGQLSGAWSHDGPFGLEWKKVPERHLSPQPVEELVAEEPVPEVSVLVLSPRCWPVSPLCYLHEPTKYFPPALSTSLTRFSNFYSQSQNRPVLGAQRLLQWSWLGRAELSFGAQTLHVSTLQMWLLLHFNQMEEVALETLLQTSDLPSELLMQALKPLTSGLGPLTLQEGQGLPPGGVLRLPEVEEEPGGEALWLLPPQTYLSVEEDECRTLERKRNLLSCLLVRILKAHGEKGLHIDQLVCLVLEAWQKGQPSPRDPGNSTGGEDECNGADVLSCILHLLGQGYVRRQEDRPQVLAYAAPEPEGPALAPPNLVALGPAPPSTFHTLEIRTRTTASPSPPARTFSTFR

Target ORF information:

RefSeq Version	XM_031968620.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii cullin 7 (CUL7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031968620.1

ORF Insert Sequence:

ATGGTGGGAG AACGCAGAAG CAGGGACCTG CTGGTGCCCC TGGGCCCACG GCTGCAGGCG 
TACCCCGAGG AACTGCTGCG GCAGCGCCAG GGCCACGATG GGCATCCAGA GTACTTGATC 
CGTTGGACCG TCCTTCAGCG CGGGAAGGAG GGCGGGGTGG GCAGCGGCAG CTCCATAGAG 
GGCAAGGCGG AGCACATCCT CATGTGGCTC TCTGCCCCCG AGGTCTACGC CAACTGCCCC 
ATGCTCCTTG GCGAGCGCGC GCCAGCCAAG GGGCCCCCGC ACGAACCCGT GGGCGGCGTG 
GGCGCCTTTC CCCGGGATGC CGGGGGGCTG GACGAAGCCT CGCTGGGGGA GATGGCCGCA 
GACGTGCGAG GACTGGTGCG TCGAGCCGCC CGGCAGCTGG ATGGGGGGGG GGGCTCGAGT 
CCCGCGGCCT CGGTGCTACA CACCATCCAC GTGCTCAGCG CCTACGCCAG CATCGGACCC 
CTGGCTGGCG TCTTCCGGGA GACGGGAGCC TTGGACCTGC TCATGCACAT GCTGGGCAAC 
CCCGAGTCCC AGATCCGCAG GAGTGCCGGG AAGATGCTGC AGGCCTTGGC AGCCCACGAC 
GCTGGGAGCA GGGCCCATGT GCTCCTGTCA CTGAGCCAGC AGGATGGCAT CGAGCAGCAC 
ATGGACTTTG ACAGCCGCTC CACTCTGTTG GAGCTGTTTG CAGAGACCAC ATCCTCCGAA 
GAGCACTGCA TGGCCTTTGA AGGGATCCAC CTGCCCCAGA TCCCCGGAAA GCTGCTCTTC 
TCCTTAGTGA AGCGTTACTT GTGTGTCACG TCACTCCTGG ACCAGCTAAG TAGCAGCGTG 
GAGCCAGGGT CTGGGGAGTG GGGAAACCGG TGCTCCCCTA ACAAGTCCTC CAGTGTGGAG 
AAGAGCCGTG GCCAGAGGGA ACTGGAGTTT AGCATGGCCA TGGGCAACCT GATTTCAGAA 
TTGGTTCGGG GCATGGGATG GGACCGGGGC CCGGGGAGGC AGCCTGCGTC TCCCCCTCGG 
CCCGCCCGGT CTATCTTTCA GCCCAGGTCC TCTGTGCCGA GCACCATCCT CCCCACCCTG 
CTTCCCCCTG CTTCCCCCAG GAGGCCGAGC CGGGCCTTCA GGCCTCGGTC CGAGTTCTCG 
AGCCTGAGTG GCTATGCGGA CTACGTCCAG GAGACCCTGC AGCCAGGGAT GCGCGTTCGA 
ATGCTGGAGG ATTATGAGGA GATCAGTGCC GGGGACCAAG GCGAGTTTCG GCAGAGCAAC 
AATGGAATGC CCCCTGTGCA GGTGCTGTGG CAGTCGACAG GCCGCACATA CTGGGTGCAC 
TGGCACATGG TGGAGATTCT GGGTCCTGTA ACTGAGGACA AAGTTACTAT TGAGCCAGAG 
AAAGGAACTG GGGGCAGCAG GTGCCTGGCC CCAAGTATAG CACGTCCATT CTTAGAGTGG 
AAACCTATTG TGGGACTGTA TCCTCTGCCG TACCTTAAAC CTGAGGCTCA GAGCAACACA 
GAGTCTGGGT GCCTGAGCCA GGCCGAATGG TGGGAACTTC TTTTCTTCAT CAAAAAGCTG 
GATGGGCCTG AGCAACAGGA GTTTCTCCGG CTCCTCCAGG AGAACCCGGA TGGGGAGCCT 
GTGGAAGAAG AAGCCCTTGG TGAACTCAAA GTATCTGTGG AGTTGGCAGA GAAACTGGTC 
CTGGCACTCA GCAGGCAGGT TCAAGGCAGC ATCCACAATG ACCTGCTTAG TTCGATTGTT 
TACAACAAAT TCAGGTTACA GCAGGTGGAG GATTTGGCCA ACCCTACTCC TCCAGAGCCT 
GGTCAGGACA CCTGCAGCCC TGAGGCCCCA TTTCCAGACA CCAAATCAGA AGTGGAGGTG 
AAAGGGCAGA TTAAATCACT AGGGGTGGAG GAGTCATCAA CAGCACCGGA AGTTGAGCTG 
CTGTTTCGGA GCCTCGTGGG GGCTGCAGAG CCCTCTGGAC AGCTGCTCCT GGAGCTGGAG 
CTAAGCATAT GCCAGGAGGG CACTGGGGAG GGTCAAGTGA GCCAGCTCCT GGGGCAGCTC 
CAACACCAGC CCCAAGCCTT CCTGCTGCTA CTACAGAGCC TGGAGACCCC CGAAAACAAG 
GCCCTTCAAA TGACTGGGCT GAGAATACTG AGCTGTCTCC TGGATGTCTC TGTGGACTCA 
AGTCTTCCTT GGCACAAAGT GGCCTCTTCC TGTTTGGCCA GCCTGAGAAC ACTCAGCAAA 
GACCATGAGG TGGTCCAAGG GTTAATCTGT TTCCTGCATC GCCTGATCAC ACTACACAAG 
GACTGCGCAG TGGTTCTGTA CCAGCTCGGA GCTCAAGAGA TTCTCACCAA GGCCCTGGAC 
AAGCACAAGG AACAACTGTT TCTGGGCTCT GAGCTCCAAG ATCTAGTGGG AGAGTATGAG 
AAGCACGCCC AGCTTTACAG CAACCTCACC AAAAGCATCT TGGCCGGCTG CATCCAGATG 
GTGCTCGGCC AGATTGAAGA GCACCGAAGA ACCCACCGGC CCATTAACAT CCCTTTCTTT 
GACGTCTTCC TCAGGCATCT TTGCCAGGGC TCTGATGTGG AGATGAAGGA CAAGTGTTGG 
GAGAAGATGG AGGTGTCGTC TAATTCTCTC GGGGCCAACA AACTGACTGA CCGAAACCCC 
AAGACTTACT GGGAATCCAG CGGCACTACA GGGTCTCACT ACATCACATT ACACATGCAT 
CAAGGGGCCA TTGTCAGACA GCTGACTCTC CTGGTGTCTA AGGAAGACTC AAGCTACATG 
CCGGCCCGGG TGGTGGTGCT GGGGGGAGAC CATGCCCGCT CTGTCAGCAC AAAGCTCAGC 
ACTGTAAATG TCATGCCTTC TGACACGCGG GTGCTTCTTC TGGAGAACAT GACCCGCTTC 
TGGCCCATCC TACAGATCCA TATCAAGCGC TGCCAGCAGG GTGGCATTGA CACCCGTGTG 
CGAGGGGTGG AGGTTCTGGG TCCCAAACCC ACTTTCTGGC CACTGTTCCG TGAGCAGCTG 
TGTCGCCGCA CTCACCTTTT CTACACCATA CGAGCCCAAG CCTGGAGTCA GGATATTGCT 
CAGGACCGGA AGCGCCTGCT GCAGCTCTGC CCTAGACTGA ACGGGGCTTT GCGCCATGAG 
CAGAGTTTTG CAGATCGATT TCTTCCTGAT GATGAGGCTG CCCAGGCACT GGGAAGGACC 
TGCTGGGAAG CCTTGGTCAG CCCCTTGGTG CAGAGCATCA CTGCCCCTGA TGGCAGTGGG 
GTAAGCCCCC TGGCCTGGCT GCTGAGCCAG TATCTGGAGC ACAGAGAGAA GGTCCAGCAC 
AAACAGGAAC AAGCAGATGC TTTTACCTCA CGTGTCCGAT GTCTCACTCA CCTGCTGGTG 
CATGTGGAGC CTCCCTCGGG TCTCCAGTCC AAGTTACTCA CCTGGCCTAA TGGCAAGAAT 
AGTAAGAGTC GGGAGCTGAA CTCGGGGTTG GGATTGGGGA CCTGGGGGGT CCCGGCCCGA 
TGGACCGAAG GGAGCCTTGG GGGGGGGCAG CCTTCCGCTG GCCCTGCGCC AGGGCTTTCT 
CTGGGGTTTC TCAAGCCTTC CTTCCTCACC GGCCCACGTG AGGAGATGGG CCGGGCAATG 
CCACAGATCC AGAGGAGGTG GTGGCCCCTA GGGGAAGAGA TGGGCCAGCT CAGCGGAGCC 
TGGAGCCATG ATGGTCCTTT CGGCCTAGAG TGGAAGAAGG TCCCTGAGAG ACATTTGTCC 
CCACAGCCAG TGGAGGAGCT GGTGGCTGAG GAGCCAGTCC CTGAAGTGTC TGTGCTGGTC 
CTGTCTCCCC GCTGCTGGCC CGTGTCCCCC CTCTGCTACC TGCACGAGCC TACCAAGTAC 
TTCCCCCCGG CTCTCAGCAC CAGCCTCACC CGGTTTTCCA ACTTCTACAG TCAGAGTCAG 
AATCGGCCAG TCTTGGGGGC ACAGCGCCTA CTGCAGTGGT CATGGTTGGG CAGGGCCGAA 
CTGTCATTTG GAGCTCAGAC TCTTCATGTC TCTACTTTAC AGATGTGGCT TCTTCTGCAT 
TTTAACCAAA TGGAGGAGGT GGCCTTGGAG ACGCTGCTGC AGACCTCAGA CCTGCCCTCA 
GAGCTTCTGA TGCAAGCACT TAAGCCCCTC ACTTCAGGGC TTGGCCCCCT GACCCTCCAG 
GAAGGCCAGG GTCTCCCACC CGGGGGTGTG CTGCGGCTTC CGGAGGTGGA GGAGGAGCCT 
GGGGGGGAGG CCCTGTGGCT GCTCCCCCCC CAGACATACC TGAGTGTGGA GGAGGATGAA 
TGCCGAACCC TGGAGAGGAA GAGGAACCTC CTGAGCTGCC TCCTTGTCCG AATCCTCAAA 
GCGCACGGCG AGAAAGGCCT GCACATTGAC CAGCTTGTCT GTCTGGTGCT GGAGGCCTGG 
CAGAAGGGCC AGCCTTCCCC CAGAGACCCC GGCAACAGCA CGGGCGGTGA GGACGAGTGT 
AATGGGGCCG ACGTCCTCTC CTGCATCCTC CACCTCCTGG GCCAGGGCTA TGTTCGGAGG 
CAGGAGGACC GGCCCCAAGT CCTGGCATAT GCAGCCCCGG AGCCCGAGGG GCCTGCCCTT 
GCACCCCCCA ACCTGGTTGC CTTGGGGCCT GCCCCACCCT CTACCTTCCA CACCCTGGAG 
ATCCGAACCC GAACCACCGC CAGCCCCTCC CCTCCTGCTC GGACTTTTTC CACCTTCCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100932280 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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