POLA1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLA1
Entrez Gene ID	100924638
Full Name	DNA polymerase alpha 1, catalytic subunit
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012544450.1	XP_012399904.1	DNA polymerase alpha catalytic subunit isoform X2
XM_031959461.1	XP_031815321.1	DNA polymerase alpha catalytic subunit isoform X2
XM_031959462.1	XP_031815322.1	DNA polymerase alpha catalytic subunit isoform X3
XM_012544449.1	XP_012399903.1	DNA polymerase alpha catalytic subunit isoform X1
XM_012544449.3	XP_012399903.1	DNA polymerase alpha catalytic subunit isoform X1
XM_012544450.2	XP_012399904.1	DNA polymerase alpha catalytic subunit isoform X2
XM_012544449.2	XP_012399903.1	DNA polymerase alpha catalytic subunit isoform X1

Pathways from BioSystems
Gene Ontology

The following POLA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa125289	XM_012544449.3 Latest version!	Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa125290	XM_012544450.2 Latest version!	Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa229420	XM_031959461.1 Latest version!	Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa229421	XM_031959462.1 Latest version!	Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OSa125289	XM_012544449.1	Sarcophilus harrisii polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa125290	XM_012544450.1	Sarcophilus harrisii polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa125289	XM_012544449.2	Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLA1 ( XM_012544449.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012399903.1 Sarcophilus harrisii polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa125289
Clone ID Related Accession (Same CDS sequence)	XM_012544449.1 , XM_012544449.3 , XM_012544449.2
Accession Version	XM_012544449.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGCCA TATTGAAGAA GAAAGAATTC CTTTCCAAGA AATACCTTGA AGCATACAAA 
ATACTAAAGA GCACAGCAGA GAAGTTCTTG TCCTGGAGTG GCTATTCGGA AGTGTGTCTC 
AGCAAACTCT TCTCAGATTG CACTGTGAAG CCCTTTAGGG AATCCCAGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012399903.1
CDS	40..4533
Translation	MAPVRSAEPEPGSSGSSIASRSRREKKSAKGRQEALERLKKTKAGEKFKYEVEDFTSVYDEVDEDQYSRMVQARQDDDWIVDDDGIGYVEDGREIFDEELDDDAFDVSKKGKDDKAFQDKKNIRKAVVSKPNNIKTMFMASAGKKTADKVVDLSKDDLLGDILQDLNATTSQITPPPVMKLKKKRPTGASLNPFSVQIPKATPLGKNDVSVSRKESPVTAIHLKHDYMAKAGVVKQKDIELTQSMVVNSSPVEVQQEDASEGEETMKEEEEEQEEQKGLMDFEDGDFDEPMEDEEADIPPKVVKIQEKDKEPKKSVEPELSHLDSFFPDVSCWDKIDQEGESSIQTQEIQVDSNRLPLVKGTDGDQVFLFYWLDAYEDQYNQPGVVFLFGKVWIEPAETHVSCCVLVKNIERTLYLLPREEQIDLTTGEKKGIPVTMKDVYQEFDEKTATKYKIMKFKSKKVEKNYAFEIPDVPEKSEYLEVRYSAELPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEIKNPQLLNQPISWCKVEAMALKPELVNVVKDIAPPPLVIMSFSLKTMQNTKTHQNEVIALAALVHHSFPLDKAAPQPPFQSHFCVVSKPNDCIFPYDFKEVINKKNVKIEIAATERTLLGFFLAKVHKIDPDVIVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLKRSNMPKLGGRGGFAERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKAERVTIPSENIRNMYSDSPHLMYLLENTWKDAKFILQIMCELNVLPLALQITNIAGNVMSRTMMGGRSERNEFLLLHAFYENNYIVPDKQNFRKPQQRLGDEDEDIDGDANKSKKGRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEDQKITEDEEQENIPDLPDPDLEMGILPKEIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNRLYKMLEIDIDGIFKSLLLLKKKKYAALVVEPTSDGKYITKQELKGLDIVRRDWCDLAKETGNFVIGQILSDQNRDTIVDNIQKRLIEIGESVMNGSVPISQFEINKALTKDPQDYPDKKSLPHVHVAIWINSHGGKKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQVHPVVARICEPIDGVDAALIATWLGLDPTQFRVHQHYHKDEENDALLGGPAQLSDEEKFRDCERFKFSCPKCGTENICDSVFDGSGTQMEPSLFRCRNLDCDASPITFVIQLNNKLILDIRRYIKKYYNGWLICEEPTCQARTRRLPLQFSRSGPLCQTCMKATLKQEYSDKSLYTQLCFYRHMFDMDHALEKLITDHEKAILKKKEFLSKKYLEAYKILKSTAEKFLSWSGYSEVCLSKLFSDCTVKPFRESQN

Target ORF information:

RefSeq Version	XM_012544449.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012544449.1

ORF Insert Sequence:

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGCCA TATTGAAGAA GAAAGAATTC CTTTCCAAGA AATACCTTGA AGCATACAAA 
ATACTAAAGA GCACAGCAGA GAAGTTCTTG TCCTGGAGTG GCTATTCGGA AGTGTGTCTC 
AGCAAACTCT TCTCAGATTG CACTGTGAAG CCCTTTAGGG AATCCCAGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_012544449.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012399903.1 Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa125289
Clone ID Related Accession (Same CDS sequence)	XM_012544449.1 , XM_012544449.3 , XM_012544449.2
Accession Version	XM_012544449.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_012544449.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGCCA TATTGAAGAA GAAAGAATTC CTTTCCAAGA AATACCTTGA AGCATACAAA 
ATACTAAAGA GCACAGCAGA GAAGTTCTTG TCCTGGAGTG GCTATTCGGA AGTGTGTCTC 
AGCAAACTCT TCTCAGATTG CACTGTGAAG CCCTTTAGGG AATCCCAGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012399903.1
CDS	40..4533
Translation	MAPVRSAEPEPGSSGSSIASRSRREKKSAKGRQEALERLKKTKAGEKFKYEVEDFTSVYDEVDEDQYSRMVQARQDDDWIVDDDGIGYVEDGREIFDEELDDDAFDVSKKGKDDKAFQDKKNIRKAVVSKPNNIKTMFMASAGKKTADKVVDLSKDDLLGDILQDLNATTSQITPPPVMKLKKKRPTGASLNPFSVQIPKATPLGKNDVSVSRKESPVTAIHLKHDYMAKAGVVKQKDIELTQSMVVNSSPVEVQQEDASEGEETMKEEEEEQEEQKGLMDFEDGDFDEPMEDEEADIPPKVVKIQEKDKEPKKSVEPELSHLDSFFPDVSCWDKIDQEGESSIQTQEIQVDSNRLPLVKGTDGDQVFLFYWLDAYEDQYNQPGVVFLFGKVWIEPAETHVSCCVLVKNIERTLYLLPREEQIDLTTGEKKGIPVTMKDVYQEFDEKTATKYKIMKFKSKKVEKNYAFEIPDVPEKSEYLEVRYSAELPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEIKNPQLLNQPISWCKVEAMALKPELVNVVKDIAPPPLVIMSFSLKTMQNTKTHQNEVIALAALVHHSFPLDKAAPQPPFQSHFCVVSKPNDCIFPYDFKEVINKKNVKIEIAATERTLLGFFLAKVHKIDPDVIVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLKRSNMPKLGGRGGFAERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKAERVTIPSENIRNMYSDSPHLMYLLENTWKDAKFILQIMCELNVLPLALQITNIAGNVMSRTMMGGRSERNEFLLLHAFYENNYIVPDKQNFRKPQQRLGDEDEDIDGDANKSKKGRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEDQKITEDEEQENIPDLPDPDLEMGILPKEIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNRLYKMLEIDIDGIFKSLLLLKKKKYAALVVEPTSDGKYITKQELKGLDIVRRDWCDLAKETGNFVIGQILSDQNRDTIVDNIQKRLIEIGESVMNGSVPISQFEINKALTKDPQDYPDKKSLPHVHVAIWINSHGGKKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQVHPVVARICEPIDGVDAALIATWLGLDPTQFRVHQHYHKDEENDALLGGPAQLSDEEKFRDCERFKFSCPKCGTENICDSVFDGSGTQMEPSLFRCRNLDCDASPITFVIQLNNKLILDIRRYIKKYYNGWLICEEPTCQARTRRLPLQFSRSGPLCQTCMKATLKQEYSDKSLYTQLCFYRHMFDMDHALEKLITDHEKAILKKKEFLSKKYLEAYKILKSTAEKFLSWSGYSEVCLSKLFSDCTVKPFRESQN

Target ORF information:

RefSeq Version	XM_012544449.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012544449.3

ORF Insert Sequence:

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGCCA TATTGAAGAA GAAAGAATTC CTTTCCAAGA AATACCTTGA AGCATACAAA 
ATACTAAAGA GCACAGCAGA GAAGTTCTTG TCCTGGAGTG GCTATTCGGA AGTGTGTCTC 
AGCAAACTCT TCTCAGATTG CACTGTGAAG CCCTTTAGGG AATCCCAGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_012544449.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012399903.1 Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

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CloneID	OSa125289
Clone ID Related Accession (Same CDS sequence)	XM_012544449.1 , XM_012544449.3 , XM_012544449.2
Accession Version	XM_012544449.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012544449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGCCA TATTGAAGAA GAAAGAATTC CTTTCCAAGA AATACCTTGA AGCATACAAA 
ATACTAAAGA GCACAGCAGA GAAGTTCTTG TCCTGGAGTG GCTATTCGGA AGTGTGTCTC 
AGCAAACTCT TCTCAGATTG CACTGTGAAG CCCTTTAGGG AATCCCAGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012399903.1
CDS	71..4564
Translation	MAPVRSAEPEPGSSGSSIASRSRREKKSAKGRQEALERLKKTKAGEKFKYEVEDFTSVYDEVDEDQYSRMVQARQDDDWIVDDDGIGYVEDGREIFDEELDDDAFDVSKKGKDDKAFQDKKNIRKAVVSKPNNIKTMFMASAGKKTADKVVDLSKDDLLGDILQDLNATTSQITPPPVMKLKKKRPTGASLNPFSVQIPKATPLGKNDVSVSRKESPVTAIHLKHDYMAKAGVVKQKDIELTQSMVVNSSPVEVQQEDASEGEETMKEEEEEQEEQKGLMDFEDGDFDEPMEDEEADIPPKVVKIQEKDKEPKKSVEPELSHLDSFFPDVSCWDKIDQEGESSIQTQEIQVDSNRLPLVKGTDGDQVFLFYWLDAYEDQYNQPGVVFLFGKVWIEPAETHVSCCVLVKNIERTLYLLPREEQIDLTTGEKKGIPVTMKDVYQEFDEKTATKYKIMKFKSKKVEKNYAFEIPDVPEKSEYLEVRYSAELPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEIKNPQLLNQPISWCKVEAMALKPELVNVVKDIAPPPLVIMSFSLKTMQNTKTHQNEVIALAALVHHSFPLDKAAPQPPFQSHFCVVSKPNDCIFPYDFKEVINKKNVKIEIAATERTLLGFFLAKVHKIDPDVIVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLKRSNMPKLGGRGGFAERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKAERVTIPSENIRNMYSDSPHLMYLLENTWKDAKFILQIMCELNVLPLALQITNIAGNVMSRTMMGGRSERNEFLLLHAFYENNYIVPDKQNFRKPQQRLGDEDEDIDGDANKSKKGRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEDQKITEDEEQENIPDLPDPDLEMGILPKEIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNRLYKMLEIDIDGIFKSLLLLKKKKYAALVVEPTSDGKYITKQELKGLDIVRRDWCDLAKETGNFVIGQILSDQNRDTIVDNIQKRLIEIGESVMNGSVPISQFEINKALTKDPQDYPDKKSLPHVHVAIWINSHGGKKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQVHPVVARICEPIDGVDAALIATWLGLDPTQFRVHQHYHKDEENDALLGGPAQLSDEEKFRDCERFKFSCPKCGTENICDSVFDGSGTQMEPSLFRCRNLDCDASPITFVIQLNNKLILDIRRYIKKYYNGWLICEEPTCQARTRRLPLQFSRSGPLCQTCMKATLKQEYSDKSLYTQLCFYRHMFDMDHALEKLITDHEKAILKKKEFLSKKYLEAYKILKSTAEKFLSWSGYSEVCLSKLFSDCTVKPFRESQN

Target ORF information:

RefSeq Version	XM_012544449.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012544449.2

ORF Insert Sequence:

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGCCA TATTGAAGAA GAAAGAATTC CTTTCCAAGA AATACCTTGA AGCATACAAA 
ATACTAAAGA GCACAGCAGA GAAGTTCTTG TCCTGGAGTG GCTATTCGGA AGTGTGTCTC 
AGCAAACTCT TCTCAGATTG CACTGTGAAG CCCTTTAGGG AATCCCAGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_012544450.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012399904.1 Sarcophilus harrisii polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

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CloneID	OSa125290
Clone ID Related Accession (Same CDS sequence)	XM_012544450.1 , XM_012544450.2
Accession Version	XM_012544450.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DNA polymerase alpha catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGGAC TTCCTGCTTG GAAAAACCAT GGAAGTATTT TCCAGATCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012399904.1
CDS	40..4413
Translation	MAPVRSAEPEPGSSGSSIASRSRREKKSAKGRQEALERLKKTKAGEKFKYEVEDFTSVYDEVDEDQYSRMVQARQDDDWIVDDDGIGYVEDGREIFDEELDDDAFDVSKKGKDDKAFQDKKNIRKAVVSKPNNIKTMFMASAGKKTADKVVDLSKDDLLGDILQDLNATTSQITPPPVMKLKKKRPTGASLNPFSVQIPKATPLGKNDVSVSRKESPVTAIHLKHDYMAKAGVVKQKDIELTQSMVVNSSPVEVQQEDASEGEETMKEEEEEQEEQKGLMDFEDGDFDEPMEDEEADIPPKVVKIQEKDKEPKKSVEPELSHLDSFFPDVSCWDKIDQEGESSIQTQEIQVDSNRLPLVKGTDGDQVFLFYWLDAYEDQYNQPGVVFLFGKVWIEPAETHVSCCVLVKNIERTLYLLPREEQIDLTTGEKKGIPVTMKDVYQEFDEKTATKYKIMKFKSKKVEKNYAFEIPDVPEKSEYLEVRYSAELPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEIKNPQLLNQPISWCKVEAMALKPELVNVVKDIAPPPLVIMSFSLKTMQNTKTHQNEVIALAALVHHSFPLDKAAPQPPFQSHFCVVSKPNDCIFPYDFKEVINKKNVKIEIAATERTLLGFFLAKVHKIDPDVIVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLKRSNMPKLGGRGGFAERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKAERVTIPSENIRNMYSDSPHLMYLLENTWKDAKFILQIMCELNVLPLALQITNIAGNVMSRTMMGGRSERNEFLLLHAFYENNYIVPDKQNFRKPQQRLGDEDEDIDGDANKSKKGRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEDQKITEDEEQENIPDLPDPDLEMGILPKEIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNRLYKMLEIDIDGIFKSLLLLKKKKYAALVVEPTSDGKYITKQELKGLDIVRRDWCDLAKETGNFVIGQILSDQNRDTIVDNIQKRLIEIGESVMNGSVPISQFEINKALTKDPQDYPDKKSLPHVHVAIWINSHGGKKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQVHPVVARICEPIDGVDAALIATWLGLDPTQFRVHQHYHKDEENDALLGGPAQLSDEEKFRDCERFKFSCPKCGTENICDSVFDGSGTQMEPSLFRCRNLDCDASPITFVIQLNNKLILDIRRYIKKYYNGWLICEEPTCQARTRRLPLQFSRSGPLCQTCMKATLKQEYSDKSLYTQLCFYRHMFDMDHALEKLITDHEKGLPAWKNHGSIFQIT

Target ORF information:

RefSeq Version	XM_012544450.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012544450.1

ORF Insert Sequence:

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGGAC TTCCTGCTTG GAAAAACCAT GGAAGTATTT TCCAGATCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_012544450.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012399904.1 Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa125290
Clone ID Related Accession (Same CDS sequence)	XM_012544450.1 , XM_012544450.2
Accession Version	XM_012544450.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DNA polymerase alpha catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012544450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGGAC TTCCTGCTTG GAAAAACCAT GGAAGTATTT TCCAGATCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012399904.1
CDS	72..4445
Translation	MAPVRSAEPEPGSSGSSIASRSRREKKSAKGRQEALERLKKTKAGEKFKYEVEDFTSVYDEVDEDQYSRMVQARQDDDWIVDDDGIGYVEDGREIFDEELDDDAFDVSKKGKDDKAFQDKKNIRKAVVSKPNNIKTMFMASAGKKTADKVVDLSKDDLLGDILQDLNATTSQITPPPVMKLKKKRPTGASLNPFSVQIPKATPLGKNDVSVSRKESPVTAIHLKHDYMAKAGVVKQKDIELTQSMVVNSSPVEVQQEDASEGEETMKEEEEEQEEQKGLMDFEDGDFDEPMEDEEADIPPKVVKIQEKDKEPKKSVEPELSHLDSFFPDVSCWDKIDQEGESSIQTQEIQVDSNRLPLVKGTDGDQVFLFYWLDAYEDQYNQPGVVFLFGKVWIEPAETHVSCCVLVKNIERTLYLLPREEQIDLTTGEKKGIPVTMKDVYQEFDEKTATKYKIMKFKSKKVEKNYAFEIPDVPEKSEYLEVRYSAELPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEIKNPQLLNQPISWCKVEAMALKPELVNVVKDIAPPPLVIMSFSLKTMQNTKTHQNEVIALAALVHHSFPLDKAAPQPPFQSHFCVVSKPNDCIFPYDFKEVINKKNVKIEIAATERTLLGFFLAKVHKIDPDVIVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLKRSNMPKLGGRGGFAERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKAERVTIPSENIRNMYSDSPHLMYLLENTWKDAKFILQIMCELNVLPLALQITNIAGNVMSRTMMGGRSERNEFLLLHAFYENNYIVPDKQNFRKPQQRLGDEDEDIDGDANKSKKGRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEDQKITEDEEQENIPDLPDPDLEMGILPKEIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNRLYKMLEIDIDGIFKSLLLLKKKKYAALVVEPTSDGKYITKQELKGLDIVRRDWCDLAKETGNFVIGQILSDQNRDTIVDNIQKRLIEIGESVMNGSVPISQFEINKALTKDPQDYPDKKSLPHVHVAIWINSHGGKKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQVHPVVARICEPIDGVDAALIATWLGLDPTQFRVHQHYHKDEENDALLGGPAQLSDEEKFRDCERFKFSCPKCGTENICDSVFDGSGTQMEPSLFRCRNLDCDASPITFVIQLNNKLILDIRRYIKKYYNGWLICEEPTCQARTRRLPLQFSRSGPLCQTCMKATLKQEYSDKSLYTQLCFYRHMFDMDHALEKLITDHEKGLPAWKNHGSIFQIT

Target ORF information:

RefSeq Version	XM_012544450.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012544450.2

ORF Insert Sequence:

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGGAC TTCCTGCTTG GAAAAACCAT GGAAGTATTT TCCAGATCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_031959461.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031815321.1 Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa229420
Clone ID Related Accession (Same CDS sequence)	XM_031959461.1
Accession Version	XM_031959461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DNA polymerase alpha catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGGGA GAGAGAGACC GACACAGAGA GCATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031815321.1
CDS	40..4398
Translation	MAPVRSAEPEPGSSGSSIASRSRREKKSAKGRQEALERLKKTKAGEKFKYEVEDFTSVYDEVDEDQYSRMVQARQDDDWIVDDDGIGYVEDGREIFDEELDDDAFDVSKKGKDDKAFQDKKNIRKAVVSKPNNIKTMFMASAGKKTADKVVDLSKDDLLGDILQDLNATTSQITPPPVMKLKKKRPTGASLNPFSVQIPKATPLGKNDVSVSRKESPVTAIHLKHDYMAKAGVVKQKDIELTQSMVVNSSPVEVQQEDASEGEETMKEEEEEQEEQKGLMDFEDGDFDEPMEDEEADIPPKVVKIQEKDKEPKKSVEPELSHLDSFFPDVSCWDKIDQEGESSIQTQEIQVDSNRLPLVKGTDGDQVFLFYWLDAYEDQYNQPGVVFLFGKVWIEPAETHVSCCVLVKNIERTLYLLPREEQIDLTTGEKKGIPVTMKDVYQEFDEKTATKYKIMKFKSKKVEKNYAFEIPDVPEKSEYLEVRYSAELPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEIKNPQLLNQPISWCKVEAMALKPELVNVVKDIAPPPLVIMSFSLKTMQNTKTHQNEVIALAALVHHSFPLDKAAPQPPFQSHFCVVSKPNDCIFPYDFKEVINKKNVKIEIAATERTLLGFFLAKVHKIDPDVIVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLKRSNMPKLGGRGGFAERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKAERVTIPSENIRNMYSDSPHLMYLLENTWKDAKFILQIMCELNVLPLALQITNIAGNVMSRTMMGGRSERNEFLLLHAFYENNYIVPDKQNFRKPQQRLGDEDEDIDGDANKSKKGRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEDQKITEDEEQENIPDLPDPDLEMGILPKEIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNRLYKMLEIDIDGIFKSLLLLKKKKYAALVVEPTSDGKYITKQELKGLDIVRRDWCDLAKETGNFVIGQILSDQNRDTIVDNIQKRLIEIGESVMNGSVPISQFEINKALTKDPQDYPDKKSLPHVHVAIWINSHGGKKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQVHPVVARICEPIDGVDAALIATWLGLDPTQFRVHQHYHKDEENDALLGGPAQLSDEEKFRDCERFKFSCPKCGTENICDSVFDGSGTQMEPSLFRCRNLDCDASPITFVIQLNNKLILDIRRYIKKYYNGWLICEEPTCQARTRRLPLQFSRSGPLCQTCMKATLKQEYSDKSLYTQLCFYRHMFDMDHALEKLITDHEKGRERPTQRAY

Target ORF information:

RefSeq Version	XM_031959461.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031959461.1

ORF Insert Sequence:

ATGGCGCCGG TGCGCAGCGC GGAGCCCGAG CCTGGGAGCA GCGGGAGTTC CATAGCTTCC 
CGTTCTCGGA GGGAAAAAAA ATCAGCAAAA GGACGTCAGG AAGCACTAGA ACGACTGAAG 
AAGACAAAAG CTGGTGAAAA ATTTAAATAC GAGGTAGAGG ACTTTACAAG TGTTTATGAT 
GAAGTTGATG AAGATCAGTA CTCCAGAATG GTCCAGGCAC GGCAGGATGA TGACTGGATT 
GTGGATGATG ATGGCATTGG CTATGTGGAG GATGGCCGGG AGATTTTTGA TGAGGAACTT 
GACGATGATG CCTTTGATGT CAGTAAGAAA GGAAAAGATG ATAAAGCATT CCAAGACAAG 
AAGAACATAA GGAAGGCTGT GGTATCAAAG CCAAATAATA TCAAGACAAT GTTTATGGCA 
AGTGCTGGAA AGAAAACTGC AGATAAAGTT GTTGATTTGT CAAAGGATGA TTTACTAGGG 
GATATTCTGC AGGATCTTAA TGCTACGACA TCTCAGATAA CTCCACCACC TGTAATGAAG 
CTAAAGAAGA AAAGACCCAC TGGAGCTTCA CTGAATCCTT TTTCTGTGCA GATACCCAAG 
GCAACTCCTT TGGGGAAAAA TGATGTTTCT GTTTCAAGAA AGGAATCTCC TGTGACTGCT 
ATTCATCTTA AACATGATTA TATGGCCAAG GCTGGTGTTG TAAAGCAGAA GGACATTGAA 
CTGACACAAA GTATGGTAGT AAATAGTAGT CCTGTGGAGG TGCAACAGGA AGATGCTTCT 
GAAGGAGAGG AGACAATGAA AGAGGAGGAA GAGGAACAAG AGGAGCAAAA GGGTTTGATG 
GATTTTGAAG ATGGAGACTT TGATGAACCT ATGGAAGATG AAGAAGCTGA CATTCCCCCC 
AAAGTTGTCA AGATCCAGGA AAAAGATAAG GAACCAAAAA AAAGTGTGGA GCCAGAGTTG 
TCACATTTAG ACAGTTTTTT CCCAGATGTT TCTTGTTGGG ACAAAATAGA TCAGGAAGGT 
GAAAGCAGTA TACAAACTCA AGAAATCCAA GTAGATTCAA ATCGCCTTCC ATTGGTTAAA 
GGGACAGATG GTGATCAAGT CTTTTTGTTT TATTGGCTGG ATGCTTATGA AGATCAGTAT 
AATCAACCAG GTGTGGTATT TCTGTTTGGC AAAGTTTGGA TTGAGCCAGC AGAGACCCAT 
GTAAGCTGTT GTGTCTTGGT AAAAAACATC GAACGGACTC TCTATCTCCT GCCCCGTGAA 
GAGCAAATAG ATCTAACTAC TGGAGAGAAG AAAGGGATTC CAGTTACAAT GAAAGATGTT 
TATCAAGAAT TTGATGAAAA GACTGCAACA AAATATAAGA TTATGAAATT TAAGTCCAAG 
AAAGTTGAAA AGAACTATGC TTTTGAAATC CCAGATGTTC CAGAAAAATC TGAATACTTG 
GAAGTAAGAT ATTCGGCTGA ATTACCACAA CTTCCTCAAG ATTTGAAAGG AGAGACATTT 
TCTCATGTAT TTGGGACTAA TACATCTAGT TTGGAACTTT TCTTGATGAA CAGAAAGATC 
AAAGGTCCTT GCTGGCTGGA AATAAAAAAT CCACAGCTCT TGAATCAACC AATCAGTTGG 
TGTAAAGTGG AGGCAATGGC TTTAAAACCA GAGTTGGTGA ATGTTGTTAA AGATATTGCT 
CCCCCTCCCC TTGTGATCAT GTCGTTCAGC TTGAAAACTA TGCAGAATAC AAAGACTCAT 
CAGAATGAGG TTATTGCTTT GGCAGCTTTG GTACATCACA GTTTTCCTTT GGATAAAGCA 
GCACCTCAGC CTCCTTTTCA GTCACACTTC TGTGTTGTGT CCAAACCAAA TGACTGTATC 
TTCCCATATG ATTTTAAAGA AGTTATTAAT AAAAAGAATG TGAAGATCGA GATTGCTGCG 
ACAGAGAGAA CATTACTGGG TTTTTTCTTG GCAAAGGTTC ACAAAATTGA CCCAGATGTC 
ATTGTGGGTC ATAATATTTA TGGATTTGAT CTGGAAGTGC TACTGCAGAG AATTAATGTG 
TGCAAAGTTC CTCACTGGTC TAAGATAGGT CGCCTAAAGA GATCCAACAT GCCAAAGCTT 
GGGGGCAGGG GTGGCTTTGC AGAAAGGAAT GCGGCCTGTG GCCGCATGAT CTGTGATGTG 
GAAATTTCAG CAAAGGAATT GATTCGTTGC AAGAGTTACC ATTTATCTGA ATTGGTTCAC 
CAGATCCTAA AAGCTGAAAG GGTAACAATT CCATCAGAAA ATATACGAAA TATGTATAGT 
GACTCTCCTC ATCTAATGTA TCTATTGGAA AATACCTGGA AAGATGCCAA GTTCATTCTG 
CAAATTATGT GTGAGCTCAA TGTCCTCCCA TTAGCACTGC AGATCACAAA TATTGCTGGG 
AATGTTATGT CAAGGACTAT GATGGGTGGT AGGTCTGAGC GTAATGAGTT CTTGCTGCTT 
CATGCATTTT ATGAGAACAA CTATATTGTG CCTGACAAAC AGAATTTCAG GAAACCTCAA 
CAAAGACTGG GAGATGAAGA CGAAGATATT GATGGAGATG CAAATAAATC CAAAAAAGGA 
CGAAAGAAAG CAGCATATGC TGGTGGCTTG GTATTGGATC CTAAAGTTGG TTTTTATGAT 
AAATTTATCT TACTTCTGGA CTTCAACAGC CTATATCCTT CAATCATTCA GGAATTTAAC 
ATTTGTTTTA CTACTGTGCA AAGAGTTGCT TCAGAGGACC AAAAAATCAC AGAGGATGAA 
GAGCAAGAAA ATATTCCTGA TTTGCCGGAT CCAGATTTAG AAATGGGCAT TTTACCCAAA 
GAAATCAGGA AACTTGTTGA GAGGAGAAAA CAAGTCAAAC AGCTCATGAA ACAGCAAGAT 
TTAAATCCAG ACCTGGTTTT ACAGTATGAC ATTAGACAGA AGGCTTTGAA GCTCACAGCA 
AATAGTATGT ACGGCTGTCT TGGATTCTCC TTTAGCAGAT TTTATGCTAA ACCATTAGCT 
GCTTTGGTGA CTTACAAAGG AAGGGAGATT TTGATGCACA CCAAGGAGAT GGTTCAAAAG 
ATGAACCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATAAATAC TAATAGCACA 
AACCTGGAAG AAGTCTTCAA GTTGGGGAAC AAGGTTAAAA GTGAGGTAAA CAGGTTGTAC 
AAGATGCTAG AGATTGATAT TGATGGCATT TTCAAGTCTT TGCTTCTGCT AAAGAAAAAG 
AAATATGCTG CTTTGGTGGT AGAGCCAACT TCAGATGGGA AATATATTAC CAAACAAGAG 
CTGAAAGGAC TGGACATAGT GAGAAGAGAT TGGTGTGATC TTGCCAAGGA AACGGGAAAC 
TTTGTCATTG GTCAGATTCT TTCTGATCAG AATAGGGACA CAATTGTGGA CAACATTCAG 
AAACGGTTAA TAGAAATTGG AGAAAGTGTG ATGAATGGTA GTGTCCCAAT AAGCCAATTT 
GAAATTAACA AGGCATTGAC AAAGGATCCT CAGGATTACC CTGACAAGAA AAGCCTACCA 
CATGTTCATG TTGCTATATG GATAAACTCT CATGGAGGCA AAAAAGTGAA AGCTGGAGAT 
ACTGTGTCAT ATATTATTTG TCAGGATGGA TCCAACCTAA GTGCCAGTCA AAGAGCTTAT 
GCCCCTGAGC AGCTGCAAAA ACAAGACAAT CTGACCATCG ATACTCAGTA CTACCTGTCA 
CAACAGGTCC ACCCTGTTGT GGCTCGAATC TGTGAGCCAA TAGATGGTGT TGATGCTGCT 
CTTATTGCAA CGTGGTTAGG ACTGGATCCC ACTCAATTCA GAGTTCATCA ACATTACCAT 
AAAGATGAAG AGAATGATGC CTTACTTGGT GGCCCAGCAC AACTCAGTGA TGAAGAAAAA 
TTTAGGGATT GTGAAAGATT CAAATTTTCT TGTCCTAAAT GTGGAACTGA AAACATTTGT 
GATAGTGTTT TTGATGGTTC GGGAACCCAG ATGGAACCCA GCTTGTTTCG TTGCCGTAAT 
TTAGATTGTG ATGCCTCACC TATAACCTTT GTCATCCAGC TGAACAATAA ATTAATCCTG 
GATATCAGGC GATACATTAA AAAATACTAC AATGGTTGGT TGATATGTGA GGAGCCAACA 
TGTCAGGCTA GAACTCGTAG ACTTCCCCTT CAGTTCTCCC GAAGTGGACC TCTCTGTCAG 
ACATGCATGA AAGCTACCCT CAAACAAGAG TATTCCGACA AGTCTCTGTA TACCCAGCTG 
TGCTTTTATC GGCACATGTT TGATATGGAC CATGCACTGG AGAAACTTAT TACTGATCAT 
GAGAAAGGGA GAGAGAGACC GACACAGAGA GCATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_031959462.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031815322.1 Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

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CloneID	OSa229421
Clone ID Related Accession (Same CDS sequence)	XM_031959462.1
Accession Version	XM_031959462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DNA polymerase alpha catalytic subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CACGGCAGGA TGATGACTGG ATTGTGGATG ATGATGGCAT TGGCTATGTG 
GAGGATGGCC GGGAGATTTT TGATGAGGAA CTTGACGATG ATGCCTTTGA TGTCAGTAAG 
AAAGGAAAAG ATGATAAAGC ATTCCAAGAC AAGAAGAACA TAAGGAAGGC TGTGGTATCA 
AAGCCAAATA ATATCAAGAC AATGTTTATG GCAAGTGCTG GAAAGAAAAC TGCAGATAAA 
GTTGTTGATT TGTCAAAGGA TGATTTACTA GGGGATATTC TGCAGGATCT TAATGCTACG 
ACATCTCAGA TAACTCCACC ACCTGTAATG AAGCTAAAGA AGAAAAGACC CACTGGAGCT 
TCACTGAATC CTTTTTCTGT GCAGATACCC AAGGCAACTC CTTTGGGGAA AAATGATGTT 
TCTGTTTCAA GAAAGGAATC TCCTGTGACT GCTATTCATC TTAAACATGA TTATATGGCC 
AAGGCTGGTG TTGTAAAGCA GAAGGACATT GAACTGACAC AAAGTATGGT AGTAAATAGT 
AGTCCTGTGG AGGTGCAACA GGAAGATGCT TCTGAAGGAG AGGAGACAAT GAAAGAGGAG 
GAAGAGGAAC AAGAGGAGCA AAAGGGTTTG ATGGATTTTG AAGATGGAGA CTTTGATGAA 
CCTATGGAAG ATGAAGAAGC TGACATTCCC CCCAAAGTTG TCAAGATCCA GGAAAAAGAT 
AAGGAACCAA AAAAAAGTGT GGAGCCAGAG TTGTCACATT TAGACAGTTT TTTCCCAGAT 
GTTTCTTGTT GGGACAAAAT AGATCAGGAA GGTGAAAGCA GTATACAAAC TCAAGAAATC 
CAAGTAGATT CAAATCGCCT TCCATTGGTT AAAGGGACAG ATGGTGATCA AGTCTTTTTG 
TTTTATTGGC TGGATGCTTA TGAAGATCAG TATAATCAAC CAGGTGTGGT ATTTCTGTTT 
GGCAAAGTTT GGATTGAGCC AGCAGAGACC CATGTAAGCT GTTGTGTCTT GGTAAAAAAC 
ATCGAACGGA CTCTCTATCT CCTGCCCCGT GAAGAGCAAA TAGATCTAAC TACTGGAGAG 
AAGAAAGGGA TTCCAGTTAC AATGAAAGAT GTTTATCAAG AATTTGATGA AAAGACTGCA 
ACAAAATATA AGATTATGAA ATTTAAGTCC AAGAAAGTTG AAAAGAACTA TGCTTTTGAA 
ATCCCAGATG TTCCAGAAAA ATCTGAATAC TTGGAAGTAA GATATTCGGC TGAATTACCA 
CAACTTCCTC AAGATTTGAA AGGAGAGACA TTTTCTCATG TATTTGGGAC TAATACATCT 
AGTTTGGAAC TTTTCTTGAT GAACAGAAAG ATCAAAGGTC CTTGCTGGCT GGAAATAAAA 
AATCCACAGC TCTTGAATCA ACCAATCAGT TGGTGTAAAG TGGAGGCAAT GGCTTTAAAA 
CCAGAGTTGG TGAATGTTGT TAAAGATATT GCTCCCCCTC CCCTTGTGAT CATGTCGTTC 
AGCTTGAAAA CTATGCAGAA TACAAAGACT CATCAGAATG AGGTTATTGC TTTGGCAGCT 
TTGGTACATC ACAGTTTTCC TTTGGATAAA GCAGCACCTC AGCCTCCTTT TCAGTCACAC 
TTCTGTGTTG TGTCCAAACC AAATGACTGT ATCTTCCCAT ATGATTTTAA AGAAGTTATT 
AATAAAAAGA ATGTGAAGAT CGAGATTGCT GCGACAGAGA GAACATTACT GGGTTTTTTC 
TTGGCAAAGG TTCACAAAAT TGACCCAGAT GTCATTGTGG GTCATAATAT TTATGGATTT 
GATCTGGAAG TGCTACTGCA GAGAATTAAT GTGTGCAAAG TTCCTCACTG GTCTAAGATA 
GGTCGCCTAA AGAGATCCAA CATGCCAAAG CTTGGGGGCA GGGGTGGCTT TGCAGAAAGG 
AATGCGGCCT GTGGCCGCAT GATCTGTGAT GTGGAAATTT CAGCAAAGGA ATTGATTCGT 
TGCAAGAGTT ACCATTTATC TGAATTGGTT CACCAGATCC TAAAAGCTGA AAGGGTAACA 
ATTCCATCAG AAAATATACG AAATATGTAT AGTGACTCTC CTCATCTAAT GTATCTATTG 
GAAAATACCT GGAAAGATGC CAAGTTCATT CTGCAAATTA TGTGTGAGCT CAATGTCCTC 
CCATTAGCAC TGCAGATCAC AAATATTGCT GGGAATGTTA TGTCAAGGAC TATGATGGGT 
GGTAGGTCTG AGCGTAATGA GTTCTTGCTG CTTCATGCAT TTTATGAGAA CAACTATATT 
GTGCCTGACA AACAGAATTT CAGGAAACCT CAACAAAGAC TGGGAGATGA AGACGAAGAT 
ATTGATGGAG ATGCAAATAA ATCCAAAAAA GGACGAAAGA AAGCAGCATA TGCTGGTGGC 
TTGGTATTGG ATCCTAAAGT TGGTTTTTAT GATAAATTTA TCTTACTTCT GGACTTCAAC 
AGCCTATATC CTTCAATCAT TCAGGAATTT AACATTTGTT TTACTACTGT GCAAAGAGTT 
GCTTCAGAGG ACCAAAAAAT CACAGAGGAT GAAGAGCAAG AAAATATTCC TGATTTGCCG 
GATCCAGATT TAGAAATGGG CATTTTACCC AAAGAAATCA GGAAACTTGT TGAGAGGAGA 
AAACAAGTCA AACAGCTCAT GAAACAGCAA GATTTAAATC CAGACCTGGT TTTACAGTAT 
GACATTAGAC AGAAGGCTTT GAAGCTCACA GCAAATAGTA TGTACGGCTG TCTTGGATTC 
TCCTTTAGCA GATTTTATGC TAAACCATTA GCTGCTTTGG TGACTTACAA AGGAAGGGAG 
ATTTTGATGC ACACCAAGGA GATGGTTCAA AAGATGAACC TTGAAGTTAT TTATGGAGAT 
ACAGATTCAA TTATGATAAA TACTAATAGC ACAAACCTGG AAGAAGTCTT CAAGTTGGGG 
AACAAGGTTA AAAGTGAGGT AAACAGGTTG TACAAGATGC TAGAGATTGA TATTGATGGC 
ATTTTCAAGT CTTTGCTTCT GCTAAAGAAA AAGAAATATG CTGCTTTGGT GGTAGAGCCA 
ACTTCAGATG GGAAATATAT TACCAAACAA GAGCTGAAAG GACTGGACAT AGTGAGAAGA 
GATTGGTGTG ATCTTGCCAA GGAAACGGGA AACTTTGTCA TTGGTCAGAT TCTTTCTGAT 
CAGAATAGGG ACACAATTGT GGACAACATT CAGAAACGGT TAATAGAAAT TGGAGAAAGT 
GTGATGAATG GTAGTGTCCC AATAAGCCAA TTTGAAATTA ACAAGGCATT GACAAAGGAT 
CCTCAGGATT ACCCTGACAA GAAAAGCCTA CCACATGTTC ATGTTGCTAT ATGGATAAAC 
TCTCATGGAG GCAAAAAAGT GAAAGCTGGA GATACTGTGT CATATATTAT TTGTCAGGAT 
GGATCCAACC TAAGTGCCAG TCAAAGAGCT TATGCCCCTG AGCAGCTGCA AAAACAAGAC 
AATCTGACCA TCGATACTCA GTACTACCTG TCACAACAGG TCCACCCTGT TGTGGCTCGA 
ATCTGTGAGC CAATAGATGG TGTTGATGCT GCTCTTATTG CAACGTGGTT AGGACTGGAT 
CCCACTCAAT TCAGAGTTCA TCAACATTAC CATAAAGATG AAGAGAATGA TGCCTTACTT 
GGTGGCCCAG CACAACTCAG TGATGAAGAA AAATTTAGGG ATTGTGAAAG ATTCAAATTT 
TCTTGTCCTA AATGTGGAAC TGAAAACATT TGTGATAGTG TTTTTGATGG TTCGGGAACC 
CAGATGGAAC CCAGCTTGTT TCGTTGCCGT AATTTAGATT GTGATGCCTC ACCTATAACC 
TTTGTCATCC AGCTGAACAA TAAATTAATC CTGGATATCA GGCGATACAT TAAAAAATAC 
TACAATGGTT GGTTGATATG TGAGGAGCCA ACATGTCAGG CTAGAACTCG TAGACTTCCC 
CTTCAGTTCT CCCGAAGTGG ACCTCTCTGT CAGACATGCA TGAAAGCTAC CCTCAAACAA 
GAGTATTCCG ACAAGTCTCT GTATACCCAG CTGTGCTTTT ATCGGCACAT GTTTGATATG 
GACCATGCAC TGGAGAAACT TATTACTGAT CATGAGAAAG CCATATTGAA GAAGAAAGAA 
TTCCTTTCCA AGAAATACCT TGAAGCATAC AAAATACTAA AGAGCACAGC AGAGAAGTTC 
TTGTCCTGGA GTGGCTATTC GGAAGTGTGT CTCAGCAAAC TCTTCTCAGA TTGCACTGTG 
AAGCCCTTTA GGGAATCCCA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031815322.1
CDS	408..4694
Translation	MVQARQDDDWIVDDDGIGYVEDGREIFDEELDDDAFDVSKKGKDDKAFQDKKNIRKAVVSKPNNIKTMFMASAGKKTADKVVDLSKDDLLGDILQDLNATTSQITPPPVMKLKKKRPTGASLNPFSVQIPKATPLGKNDVSVSRKESPVTAIHLKHDYMAKAGVVKQKDIELTQSMVVNSSPVEVQQEDASEGEETMKEEEEEQEEQKGLMDFEDGDFDEPMEDEEADIPPKVVKIQEKDKEPKKSVEPELSHLDSFFPDVSCWDKIDQEGESSIQTQEIQVDSNRLPLVKGTDGDQVFLFYWLDAYEDQYNQPGVVFLFGKVWIEPAETHVSCCVLVKNIERTLYLLPREEQIDLTTGEKKGIPVTMKDVYQEFDEKTATKYKIMKFKSKKVEKNYAFEIPDVPEKSEYLEVRYSAELPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEIKNPQLLNQPISWCKVEAMALKPELVNVVKDIAPPPLVIMSFSLKTMQNTKTHQNEVIALAALVHHSFPLDKAAPQPPFQSHFCVVSKPNDCIFPYDFKEVINKKNVKIEIAATERTLLGFFLAKVHKIDPDVIVGHNIYGFDLEVLLQRINVCKVPHWSKIGRLKRSNMPKLGGRGGFAERNAACGRMICDVEISAKELIRCKSYHLSELVHQILKAERVTIPSENIRNMYSDSPHLMYLLENTWKDAKFILQIMCELNVLPLALQITNIAGNVMSRTMMGGRSERNEFLLLHAFYENNYIVPDKQNFRKPQQRLGDEDEDIDGDANKSKKGRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEDQKITEDEEQENIPDLPDPDLEMGILPKEIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNRLYKMLEIDIDGIFKSLLLLKKKKYAALVVEPTSDGKYITKQELKGLDIVRRDWCDLAKETGNFVIGQILSDQNRDTIVDNIQKRLIEIGESVMNGSVPISQFEINKALTKDPQDYPDKKSLPHVHVAIWINSHGGKKVKAGDTVSYIICQDGSNLSASQRAYAPEQLQKQDNLTIDTQYYLSQQVHPVVARICEPIDGVDAALIATWLGLDPTQFRVHQHYHKDEENDALLGGPAQLSDEEKFRDCERFKFSCPKCGTENICDSVFDGSGTQMEPSLFRCRNLDCDASPITFVIQLNNKLILDIRRYIKKYYNGWLICEEPTCQARTRRLPLQFSRSGPLCQTCMKATLKQEYSDKSLYTQLCFYRHMFDMDHALEKLITDHEKAILKKKEFLSKKYLEAYKILKSTAEKFLSWSGYSEVCLSKLFSDCTVKPFRESQN

Target ORF information:

RefSeq Version	XM_031959462.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031959462.1

ORF Insert Sequence:

ATGGTCCAGG CACGGCAGGA TGATGACTGG ATTGTGGATG ATGATGGCAT TGGCTATGTG 
GAGGATGGCC GGGAGATTTT TGATGAGGAA CTTGACGATG ATGCCTTTGA TGTCAGTAAG 
AAAGGAAAAG ATGATAAAGC ATTCCAAGAC AAGAAGAACA TAAGGAAGGC TGTGGTATCA 
AAGCCAAATA ATATCAAGAC AATGTTTATG GCAAGTGCTG GAAAGAAAAC TGCAGATAAA 
GTTGTTGATT TGTCAAAGGA TGATTTACTA GGGGATATTC TGCAGGATCT TAATGCTACG 
ACATCTCAGA TAACTCCACC ACCTGTAATG AAGCTAAAGA AGAAAAGACC CACTGGAGCT 
TCACTGAATC CTTTTTCTGT GCAGATACCC AAGGCAACTC CTTTGGGGAA AAATGATGTT 
TCTGTTTCAA GAAAGGAATC TCCTGTGACT GCTATTCATC TTAAACATGA TTATATGGCC 
AAGGCTGGTG TTGTAAAGCA GAAGGACATT GAACTGACAC AAAGTATGGT AGTAAATAGT 
AGTCCTGTGG AGGTGCAACA GGAAGATGCT TCTGAAGGAG AGGAGACAAT GAAAGAGGAG 
GAAGAGGAAC AAGAGGAGCA AAAGGGTTTG ATGGATTTTG AAGATGGAGA CTTTGATGAA 
CCTATGGAAG ATGAAGAAGC TGACATTCCC CCCAAAGTTG TCAAGATCCA GGAAAAAGAT 
AAGGAACCAA AAAAAAGTGT GGAGCCAGAG TTGTCACATT TAGACAGTTT TTTCCCAGAT 
GTTTCTTGTT GGGACAAAAT AGATCAGGAA GGTGAAAGCA GTATACAAAC TCAAGAAATC 
CAAGTAGATT CAAATCGCCT TCCATTGGTT AAAGGGACAG ATGGTGATCA AGTCTTTTTG 
TTTTATTGGC TGGATGCTTA TGAAGATCAG TATAATCAAC CAGGTGTGGT ATTTCTGTTT 
GGCAAAGTTT GGATTGAGCC AGCAGAGACC CATGTAAGCT GTTGTGTCTT GGTAAAAAAC 
ATCGAACGGA CTCTCTATCT CCTGCCCCGT GAAGAGCAAA TAGATCTAAC TACTGGAGAG 
AAGAAAGGGA TTCCAGTTAC AATGAAAGAT GTTTATCAAG AATTTGATGA AAAGACTGCA 
ACAAAATATA AGATTATGAA ATTTAAGTCC AAGAAAGTTG AAAAGAACTA TGCTTTTGAA 
ATCCCAGATG TTCCAGAAAA ATCTGAATAC TTGGAAGTAA GATATTCGGC TGAATTACCA 
CAACTTCCTC AAGATTTGAA AGGAGAGACA TTTTCTCATG TATTTGGGAC TAATACATCT 
AGTTTGGAAC TTTTCTTGAT GAACAGAAAG ATCAAAGGTC CTTGCTGGCT GGAAATAAAA 
AATCCACAGC TCTTGAATCA ACCAATCAGT TGGTGTAAAG TGGAGGCAAT GGCTTTAAAA 
CCAGAGTTGG TGAATGTTGT TAAAGATATT GCTCCCCCTC CCCTTGTGAT CATGTCGTTC 
AGCTTGAAAA CTATGCAGAA TACAAAGACT CATCAGAATG AGGTTATTGC TTTGGCAGCT 
TTGGTACATC ACAGTTTTCC TTTGGATAAA GCAGCACCTC AGCCTCCTTT TCAGTCACAC 
TTCTGTGTTG TGTCCAAACC AAATGACTGT ATCTTCCCAT ATGATTTTAA AGAAGTTATT 
AATAAAAAGA ATGTGAAGAT CGAGATTGCT GCGACAGAGA GAACATTACT GGGTTTTTTC 
TTGGCAAAGG TTCACAAAAT TGACCCAGAT GTCATTGTGG GTCATAATAT TTATGGATTT 
GATCTGGAAG TGCTACTGCA GAGAATTAAT GTGTGCAAAG TTCCTCACTG GTCTAAGATA 
GGTCGCCTAA AGAGATCCAA CATGCCAAAG CTTGGGGGCA GGGGTGGCTT TGCAGAAAGG 
AATGCGGCCT GTGGCCGCAT GATCTGTGAT GTGGAAATTT CAGCAAAGGA ATTGATTCGT 
TGCAAGAGTT ACCATTTATC TGAATTGGTT CACCAGATCC TAAAAGCTGA AAGGGTAACA 
ATTCCATCAG AAAATATACG AAATATGTAT AGTGACTCTC CTCATCTAAT GTATCTATTG 
GAAAATACCT GGAAAGATGC CAAGTTCATT CTGCAAATTA TGTGTGAGCT CAATGTCCTC 
CCATTAGCAC TGCAGATCAC AAATATTGCT GGGAATGTTA TGTCAAGGAC TATGATGGGT 
GGTAGGTCTG AGCGTAATGA GTTCTTGCTG CTTCATGCAT TTTATGAGAA CAACTATATT 
GTGCCTGACA AACAGAATTT CAGGAAACCT CAACAAAGAC TGGGAGATGA AGACGAAGAT 
ATTGATGGAG ATGCAAATAA ATCCAAAAAA GGACGAAAGA AAGCAGCATA TGCTGGTGGC 
TTGGTATTGG ATCCTAAAGT TGGTTTTTAT GATAAATTTA TCTTACTTCT GGACTTCAAC 
AGCCTATATC CTTCAATCAT TCAGGAATTT AACATTTGTT TTACTACTGT GCAAAGAGTT 
GCTTCAGAGG ACCAAAAAAT CACAGAGGAT GAAGAGCAAG AAAATATTCC TGATTTGCCG 
GATCCAGATT TAGAAATGGG CATTTTACCC AAAGAAATCA GGAAACTTGT TGAGAGGAGA 
AAACAAGTCA AACAGCTCAT GAAACAGCAA GATTTAAATC CAGACCTGGT TTTACAGTAT 
GACATTAGAC AGAAGGCTTT GAAGCTCACA GCAAATAGTA TGTACGGCTG TCTTGGATTC 
TCCTTTAGCA GATTTTATGC TAAACCATTA GCTGCTTTGG TGACTTACAA AGGAAGGGAG 
ATTTTGATGC ACACCAAGGA GATGGTTCAA AAGATGAACC TTGAAGTTAT TTATGGAGAT 
ACAGATTCAA TTATGATAAA TACTAATAGC ACAAACCTGG AAGAAGTCTT CAAGTTGGGG 
AACAAGGTTA AAAGTGAGGT AAACAGGTTG TACAAGATGC TAGAGATTGA TATTGATGGC 
ATTTTCAAGT CTTTGCTTCT GCTAAAGAAA AAGAAATATG CTGCTTTGGT GGTAGAGCCA 
ACTTCAGATG GGAAATATAT TACCAAACAA GAGCTGAAAG GACTGGACAT AGTGAGAAGA 
GATTGGTGTG ATCTTGCCAA GGAAACGGGA AACTTTGTCA TTGGTCAGAT TCTTTCTGAT 
CAGAATAGGG ACACAATTGT GGACAACATT CAGAAACGGT TAATAGAAAT TGGAGAAAGT 
GTGATGAATG GTAGTGTCCC AATAAGCCAA TTTGAAATTA ACAAGGCATT GACAAAGGAT 
CCTCAGGATT ACCCTGACAA GAAAAGCCTA CCACATGTTC ATGTTGCTAT ATGGATAAAC 
TCTCATGGAG GCAAAAAAGT GAAAGCTGGA GATACTGTGT CATATATTAT TTGTCAGGAT 
GGATCCAACC TAAGTGCCAG TCAAAGAGCT TATGCCCCTG AGCAGCTGCA AAAACAAGAC 
AATCTGACCA TCGATACTCA GTACTACCTG TCACAACAGG TCCACCCTGT TGTGGCTCGA 
ATCTGTGAGC CAATAGATGG TGTTGATGCT GCTCTTATTG CAACGTGGTT AGGACTGGAT 
CCCACTCAAT TCAGAGTTCA TCAACATTAC CATAAAGATG AAGAGAATGA TGCCTTACTT 
GGTGGCCCAG CACAACTCAG TGATGAAGAA AAATTTAGGG ATTGTGAAAG ATTCAAATTT 
TCTTGTCCTA AATGTGGAAC TGAAAACATT TGTGATAGTG TTTTTGATGG TTCGGGAACC 
CAGATGGAAC CCAGCTTGTT TCGTTGCCGT AATTTAGATT GTGATGCCTC ACCTATAACC 
TTTGTCATCC AGCTGAACAA TAAATTAATC CTGGATATCA GGCGATACAT TAAAAAATAC 
TACAATGGTT GGTTGATATG TGAGGAGCCA ACATGTCAGG CTAGAACTCG TAGACTTCCC 
CTTCAGTTCT CCCGAAGTGG ACCTCTCTGT CAGACATGCA TGAAAGCTAC CCTCAAACAA 
GAGTATTCCG ACAAGTCTCT GTATACCCAG CTGTGCTTTT ATCGGCACAT GTTTGATATG 
GACCATGCAC TGGAGAAACT TATTACTGAT CATGAGAAAG CCATATTGAA GAAGAAAGAA 
TTCCTTTCCA AGAAATACCT TGAAGCATAC AAAATACTAA AGAGCACAGC AGAGAAGTTC 
TTGTCCTGGA GTGGCTATTC GGAAGTGTGT CTCAGCAAAC TCTTCTCAGA TTGCACTGTG 
AAGCCCTTTA GGGAATCCCA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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POLA1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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