SKIV2L cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	SKIV2L
Entrez Gene ID	100923697
Full Name	Ski2 like RNA helicase
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003768913.3	XP_003768961.1	helicase SKI2W
XM_003768913.2	XP_003768961.1	helicase SKI2W
XM_003768913.4	XP_003768961.1	helicase SKI2W isoform X1
XM_031965145.1	XP_031821005.1	helicase SKI2W isoform X2

Pathways from BioSystems
Gene Ontology

The following SKIV2L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SKIV2L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa131050	XM_003768913.4 Latest version!	Sarcophilus harrisii Ski2 like RNA helicase (SKIV2L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa233174	XM_031965145.1 Latest version!	Sarcophilus harrisii Ski2 like RNA helicase (SKIV2L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OSa131050	XM_003768913.2	Sarcophilus harrisii superkiller viralicidic activity 2-like (S. cerevisiae) (SKIV2L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa131050	XM_003768913.3	Sarcophilus harrisii Ski2 like RNA helicase (SKIV2L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SKIV2L ( XM_003768913.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003768961.1 Sarcophilus harrisii Ski2 like RNA helicase (SKIV2L), mRNA.

Price & Availability↑

CloneID	OSa131050
Clone ID Related Accession (Same CDS sequence)	XM_003768913.3 , XM_003768913.2 , XM_003768913.4
Accession Version	XM_003768913.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	helicase SKI2W
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_003768913.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGA CGGAGCGTCT GGTACTGCCC CCTCGGGGGC CCCTGGATTT GCCTTTGTGT 
GCGCTGGAGC TCGGATGCAC TGGGCGCTGG GAGCTTTTGA GCCAGCCTCA GGTCTCGGAT 
TCGCCGGCGG GCACCCTCTC TCATGGGCTA CCACCCTGTG CCCCAGAACT GCAAGGAGAG 
ACTGAGCAAT TGTTTCTGTC ATCCCCTGAA TGGCTGCCCC TGCATGGTGT GGAACGTGTG 
GCCTGGAAAT GGCAGAGAAA GACAGATCCC TGGTCTCTCC TGGGGCTGCC AGGAGCACCT 
GTCCCATCAG ATCTGCAGGC TCAAAGACAC CCAACCACCG GCAGAGTCCT GGGCTACAGA 
GAGGTCCTGC TGGAGGACAC AAACCTTTCG GCCACAACTT CCCTGTCTCT ACGCCGGCCC 
CCAGGACCCT CATCCCAGGC TTTATGGGGC AACCCAACAC ACTATCCATT TTGGCCAGGT 
GGGATGGATG AACCTACCAT AGCAGATCTG AGCATTTGTG GGGACTCTGA GGAAGAGATT 
GACTTTGAGA AAGATCTCCT TACCATTCCC CCAGGCTTCA AGAAAGGTGT AGGCTTCACT 
CCAAAGAATA ACTCAACCCC TGCTTCTGGC CTTCTCAGTC TTAGCCATCT TCTCGAACCC 
CTGGAACTTG GTGGAGGGGA GGAAGAGGAA GAAGAGACTT CAGGAAGATT GGGGGGTCCT 
GGAGGGGACA GTGTTACAGC CTCCACTTCT GAGCCCCCAC TGGTTCATGC TAGTAGCCTG 
GAGGATTTAG TCCTAAAGGA TGCATCAGCC CCCTTGTCAC CTCCAGAACC TCCAAAGCCC 
CCAGTCTTGG AACAGTGGGC TATTCCTGTG GATATTACTT CACCTGTGGG AGATTTCTAC 
CGGATTATCC CCCAACCTGC CTTCCAGTGG CCGTTTGAAC CAGATGTATT TCAGAAACAG 
GCTATTCTGC ACCTGGAGAG ACATGACTCT GTATTTGTTG CTGCTCACAC ATCTGCAGGA 
AAGACTGTTG TGGCTGAATA TGCCATTGCC CTTGCCCAGA AACATATGAC CCGTACCATC 
TATACATCAC CTATCAAGGC CCTGAGTAAT CAGAAATTTA GAGATTTCCG AAACACCTTT 
GGTGATGTGG GACTCCTCAC AGGGGATGTC CAGCTACATC CTGAAGCTTC CTGCCTCATT 
ATGACCACAG AGATCCTTCG CTCCATGCTG TATAGTGGTT CAGATGTGAT TCGAGACCTT 
GAATGGGTGA TATTTGATGA GGTCCATTAC ATCAATGATG CTGAGAGAGG AGTAGTGTGG 
GAAGAAGTCC TCATCATGCT TCCTGATCAT GTTTCCATCA TTCTCCTCAG TGCTACTGTG 
CCCAATGCCC TTGAATTTGC AGATTGGATT GGGAGGCTGA AGCGTCGGCA TCTCTATGTG 
ATCAGCACAG CTGCCCGACC AGTTCCCTTG GAACATTTTC TATTCACTGG GAATAGTCCA 
AAAACCCAAG GAGAGCTTTT TCTGCTTCTG GATTCACGAG GAGTTTTTCA CACTAAGGGA 
TACTATGCAG CTGTGGAGGC CAAGAAAGAA AGGACTAGCA AACATGCACA GACCTTTGGG 
GCAAAGCAGC CAATGCACCA AGGTGGGCCT GGACAGGACC GAGGCATATA CCTGTCCCTC 
TTGGCCTCCC TGCGGAGCCG TGCCCAGCTC CCTGTGGTGG TGTTCACCTT CTCCCGGGGC 
CGCTGTGATG AGCAGGCGTC AGGTCTCAGC TCCCTTGACC TTACCACCAG CTCTGAGAAG 
AGTGAGATCC ACCTTTTCCT GCAGCGATGC CTTGCACGAC TTCGAGGCTC CGACCGCCAG 
CTACCTCAGG TACTGCATAT GTCAGAACTC CTTCGCCGGG GTCTAGGTGT ACACCACAGC 
GGTATATTGC CCATTCTCAA GGAGATTGTG GAAATGCTCT TCAGTCGTGG TTTGGTCAAG 
GTCCTATTTG CTACAGAAAC CTTTGCCATG GGTGTGAACA TGCCAGCACG GACTGTGGTG 
TTTGATTCTA TGCGCAAGCA TGATGGTGCA ACTTTCCGTG ACTTGCTCCC TGGGGAATAT 
GTACAGATGG CAGGGCGGGC AGGGCGTCGT GGTCTGGACC CTACAGGCAC TGTCATCCTA 
CTCTGTAAGG GACGTGTGCC TGAGATGGCT GATCTGCATA GGATGATGTT GGGAAAGCCT 
TCCCAGCTAC AGTCCCAATT TCGCCTCACA TATACAATGA TTCTTAACTT GCTTCGGGTT 
GATGCCCTCA GAGTGGAAGA TATGATGAAG AGAAGCTTTT CTGAGTTCCC TTCAAGAAAG 
GACAGCAAGG CACATGAGCA GACTTTGGCA GAATTGACAA AGAGACTTGG GGACCTAGAG 
GAACCAGATA CCTCAGGGCA GCTTGCTGAT CTTCCTGAGT ATTACAGCTG GGGGGAAGAG 
CTGACAGAGA CTCGGGGCCT AATCCAGCGA CGGATCATGG AATCAGTGAA TGGGTTGAAA 
TCTCTGTCAG TGGGAAGAGT GGTAGTGGTT AAGAATCAGG AGCATCGGAA TGCACTTGGA 
GTGATCTTGC AGGTCTCCTC AGATACCACA AACCGAGTTT TCACAACCTT GATTCTCTGT 
GAGAAGCAGC CCCCTGAGGG GCCACTGCCT GGCAGTTCCA GATCCCTTGA TGTTCCCTAT 
CCTGATGATT TAGTGGGTCA CAAGCTATTC CTCCCTGAAG GGCCTTGTGA TCATACTGTG 
GCAAAACTTC AGCCAAGTGA CATCATTGCT ATCACAACAA AGATGCTACG GGTGAATGGG 
GAACGAATTT TGGAAGATTT TAGCAAGAGG CAGCAGCCAA GATTCAAGAA GGACCCTCCC 
TCTCCATTGG TGACCTCTGC TGTTCAGGAG ATGCTCCGAT TGGCTCAGGC ACATCCAGCG 
GGACCCCCCA CTCTGGATCC AGTCAATGAT TTACAACTGA AGGAGGTGTT AGTAGTTGAG 
GGTGGGCTCC GTGCTCGGAA GCTAGAAGAA TTGATTCGAG GTGCCCAGTG TGTACATAGT 
CCCAGGTTTC CAGCCCAGTA CCTGAGACTG CAGGAACGTG TGCAGATCCA GAAGGAGATG 
GAAAGATTAC GGTTTCTACT TTCAGACCAG TCATTGCTCC TGCTGCCAGA GTATCATCAG 
CGAGTGGAGA TCCTGAGGAC ATTGGGCTAT GTGGATGGGG CAGGGACCGT GAAGCTGGCA 
GGAAGGGTGG CATGTGCCAT GAGTAGTCAT GAACTGCTTC TGACAGAGCT CATGTTTGAC 
AATGCGCTGA GTGCCCTTCG GCCAGAAGAG ATTGCTGCTC TGCTATCTGG CCTTGTCTGC 
CAAAGTCCTG GAGATCCTGG AGACCAATTG CCAAGCACTC TTAAACAGGG CATAGAGCGT 
GTCAAGGATG TGGCCCGGCG GATTGGTGAA GTTCAAGTAT CCTGTGGCCT AAACCAGACT 
GTGGAAGAGT TTGTGGGAGA ACTACACTTT GGGCTGGTAG AAGTTGTCTA TGAGTGGGCA 
CGAGGCATGC CTTTCTCTGA GCTGGCAGGG CTATCAGGGA CCCCAGAAGG TTTGATTGTC 
AGATGTATCC AGCGCCTGGC AGAGATGTGT CGTTCATTGC GAGGGGCTGC CCGGCTGGTG 
GGAGAACCTG TGCTAGGTGC TAAGATGGAG GCAGCTGCCA CAATGCTCCG TCGTGACATA 
GTTTTTGCTG CTAGTCTCTA TACCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003768961.1
CDS	62..3811
Translation	MLETERLVLPPRGPLDLPLCALELGCTGRWELLSQPQVSDSPAGTLSHGLPPCAPELQGETEQLFLSSPEWLPLHGVERVAWKWQRKTDPWSLLGLPGAPVPSDLQAQRHPTTGRVLGYREVLLEDTNLSATTSLSLRRPPGPSSQALWGNPTHYPFWPGGMDEPTIADLSICGDSEEEIDFEKDLLTIPPGFKKGVGFTPKNNSTPASGLLSLSHLLEPLELGGGEEEEEETSGRLGGPGGDSVTASTSEPPLVHASSLEDLVLKDASAPLSPPEPPKPPVLEQWAIPVDITSPVGDFYRIIPQPAFQWPFEPDVFQKQAILHLERHDSVFVAAHTSAGKTVVAEYAIALAQKHMTRTIYTSPIKALSNQKFRDFRNTFGDVGLLTGDVQLHPEASCLIMTTEILRSMLYSGSDVIRDLEWVIFDEVHYINDAERGVVWEEVLIMLPDHVSIILLSATVPNALEFADWIGRLKRRHLYVISTAARPVPLEHFLFTGNSPKTQGELFLLLDSRGVFHTKGYYAAVEAKKERTSKHAQTFGAKQPMHQGGPGQDRGIYLSLLASLRSRAQLPVVVFTFSRGRCDEQASGLSSLDLTTSSEKSEIHLFLQRCLARLRGSDRQLPQVLHMSELLRRGLGVHHSGILPILKEIVEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSMRKHDGATFRDLLPGEYVQMAGRAGRRGLDPTGTVILLCKGRVPEMADLHRMMLGKPSQLQSQFRLTYTMILNLLRVDALRVEDMMKRSFSEFPSRKDSKAHEQTLAELTKRLGDLEEPDTSGQLADLPEYYSWGEELTETRGLIQRRIMESVNGLKSLSVGRVVVVKNQEHRNALGVILQVSSDTTNRVFTTLILCEKQPPEGPLPGSSRSLDVPYPDDLVGHKLFLPEGPCDHTVAKLQPSDIIAITTKMLRVNGERILEDFSKRQQPRFKKDPPSPLVTSAVQEMLRLAQAHPAGPPTLDPVNDLQLKEVLVVEGGLRARKLEELIRGAQCVHSPRFPAQYLRLQERVQIQKEMERLRFLLSDQSLLLLPEYHQRVEILRTLGYVDGAGTVKLAGRVACAMSSHELLLTELMFDNALSALRPEEIAALLSGLVCQSPGDPGDQLPSTLKQGIERVKDVARRIGEVQVSCGLNQTVEEFVGELHFGLVEVVYEWARGMPFSELAGLSGTPEGLIVRCIQRLAEMCRSLRGAARLVGEPVLGAKMEAAATMLRRDIVFAASLYTQ

Target ORF information:

RefSeq Version	XM_003768913.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Ski2 like RNA helicase (SKIV2L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003768913.3

ORF Insert Sequence:

ATGCTGGAGA CGGAGCGTCT GGTACTGCCC CCTCGGGGGC CCCTGGATTT GCCTTTGTGT 
GCGCTGGAGC TCGGATGCAC TGGGCGCTGG GAGCTTTTGA GCCAGCCTCA GGTCTCGGAT 
TCGCCGGCGG GCACCCTCTC TCATGGGCTA CCACCCTGTG CCCCAGAACT GCAAGGAGAG 
ACTGAGCAAT TGTTTCTGTC ATCCCCTGAA TGGCTGCCCC TGCATGGTGT GGAACGTGTG 
GCCTGGAAAT GGCAGAGAAA GACAGATCCC TGGTCTCTCC TGGGGCTGCC AGGAGCACCT 
GTCCCATCAG ATCTGCAGGC TCAAAGACAC CCAACCACCG GCAGAGTCCT GGGCTACAGA 
GAGGTCCTGC TGGAGGACAC AAACCTTTCG GCCACAACTT CCCTGTCTCT ACGCCGGCCC 
CCAGGACCCT CATCCCAGGC TTTATGGGGC AACCCAACAC ACTATCCATT TTGGCCAGGT 
GGGATGGATG AACCTACCAT AGCAGATCTG AGCATTTGTG GGGACTCTGA GGAAGAGATT 
GACTTTGAGA AAGATCTCCT TACCATTCCC CCAGGCTTCA AGAAAGGTGT AGGCTTCACT 
CCAAAGAATA ACTCAACCCC TGCTTCTGGC CTTCTCAGTC TTAGCCATCT TCTCGAACCC 
CTGGAACTTG GTGGAGGGGA GGAAGAGGAA GAAGAGACTT CAGGAAGATT GGGGGGTCCT 
GGAGGGGACA GTGTTACAGC CTCCACTTCT GAGCCCCCAC TGGTTCATGC TAGTAGCCTG 
GAGGATTTAG TCCTAAAGGA TGCATCAGCC CCCTTGTCAC CTCCAGAACC TCCAAAGCCC 
CCAGTCTTGG AACAGTGGGC TATTCCTGTG GATATTACTT CACCTGTGGG AGATTTCTAC 
CGGATTATCC CCCAACCTGC CTTCCAGTGG CCGTTTGAAC CAGATGTATT TCAGAAACAG 
GCTATTCTGC ACCTGGAGAG ACATGACTCT GTATTTGTTG CTGCTCACAC ATCTGCAGGA 
AAGACTGTTG TGGCTGAATA TGCCATTGCC CTTGCCCAGA AACATATGAC CCGTACCATC 
TATACATCAC CTATCAAGGC CCTGAGTAAT CAGAAATTTA GAGATTTCCG AAACACCTTT 
GGTGATGTGG GACTCCTCAC AGGGGATGTC CAGCTACATC CTGAAGCTTC CTGCCTCATT 
ATGACCACAG AGATCCTTCG CTCCATGCTG TATAGTGGTT CAGATGTGAT TCGAGACCTT 
GAATGGGTGA TATTTGATGA GGTCCATTAC ATCAATGATG CTGAGAGAGG AGTAGTGTGG 
GAAGAAGTCC TCATCATGCT TCCTGATCAT GTTTCCATCA TTCTCCTCAG TGCTACTGTG 
CCCAATGCCC TTGAATTTGC AGATTGGATT GGGAGGCTGA AGCGTCGGCA TCTCTATGTG 
ATCAGCACAG CTGCCCGACC AGTTCCCTTG GAACATTTTC TATTCACTGG GAATAGTCCA 
AAAACCCAAG GAGAGCTTTT TCTGCTTCTG GATTCACGAG GAGTTTTTCA CACTAAGGGA 
TACTATGCAG CTGTGGAGGC CAAGAAAGAA AGGACTAGCA AACATGCACA GACCTTTGGG 
GCAAAGCAGC CAATGCACCA AGGTGGGCCT GGACAGGACC GAGGCATATA CCTGTCCCTC 
TTGGCCTCCC TGCGGAGCCG TGCCCAGCTC CCTGTGGTGG TGTTCACCTT CTCCCGGGGC 
CGCTGTGATG AGCAGGCGTC AGGTCTCAGC TCCCTTGACC TTACCACCAG CTCTGAGAAG 
AGTGAGATCC ACCTTTTCCT GCAGCGATGC CTTGCACGAC TTCGAGGCTC CGACCGCCAG 
CTACCTCAGG TACTGCATAT GTCAGAACTC CTTCGCCGGG GTCTAGGTGT ACACCACAGC 
GGTATATTGC CCATTCTCAA GGAGATTGTG GAAATGCTCT TCAGTCGTGG TTTGGTCAAG 
GTCCTATTTG CTACAGAAAC CTTTGCCATG GGTGTGAACA TGCCAGCACG GACTGTGGTG 
TTTGATTCTA TGCGCAAGCA TGATGGTGCA ACTTTCCGTG ACTTGCTCCC TGGGGAATAT 
GTACAGATGG CAGGGCGGGC AGGGCGTCGT GGTCTGGACC CTACAGGCAC TGTCATCCTA 
CTCTGTAAGG GACGTGTGCC TGAGATGGCT GATCTGCATA GGATGATGTT GGGAAAGCCT 
TCCCAGCTAC AGTCCCAATT TCGCCTCACA TATACAATGA TTCTTAACTT GCTTCGGGTT 
GATGCCCTCA GAGTGGAAGA TATGATGAAG AGAAGCTTTT CTGAGTTCCC TTCAAGAAAG 
GACAGCAAGG CACATGAGCA GACTTTGGCA GAATTGACAA AGAGACTTGG GGACCTAGAG 
GAACCAGATA CCTCAGGGCA GCTTGCTGAT CTTCCTGAGT ATTACAGCTG GGGGGAAGAG 
CTGACAGAGA CTCGGGGCCT AATCCAGCGA CGGATCATGG AATCAGTGAA TGGGTTGAAA 
TCTCTGTCAG TGGGAAGAGT GGTAGTGGTT AAGAATCAGG AGCATCGGAA TGCACTTGGA 
GTGATCTTGC AGGTCTCCTC AGATACCACA AACCGAGTTT TCACAACCTT GATTCTCTGT 
GAGAAGCAGC CCCCTGAGGG GCCACTGCCT GGCAGTTCCA GATCCCTTGA TGTTCCCTAT 
CCTGATGATT TAGTGGGTCA CAAGCTATTC CTCCCTGAAG GGCCTTGTGA TCATACTGTG 
GCAAAACTTC AGCCAAGTGA CATCATTGCT ATCACAACAA AGATGCTACG GGTGAATGGG 
GAACGAATTT TGGAAGATTT TAGCAAGAGG CAGCAGCCAA GATTCAAGAA GGACCCTCCC 
TCTCCATTGG TGACCTCTGC TGTTCAGGAG ATGCTCCGAT TGGCTCAGGC ACATCCAGCG 
GGACCCCCCA CTCTGGATCC AGTCAATGAT TTACAACTGA AGGAGGTGTT AGTAGTTGAG 
GGTGGGCTCC GTGCTCGGAA GCTAGAAGAA TTGATTCGAG GTGCCCAGTG TGTACATAGT 
CCCAGGTTTC CAGCCCAGTA CCTGAGACTG CAGGAACGTG TGCAGATCCA GAAGGAGATG 
GAAAGATTAC GGTTTCTACT TTCAGACCAG TCATTGCTCC TGCTGCCAGA GTATCATCAG 
CGAGTGGAGA TCCTGAGGAC ATTGGGCTAT GTGGATGGGG CAGGGACCGT GAAGCTGGCA 
GGAAGGGTGG CATGTGCCAT GAGTAGTCAT GAACTGCTTC TGACAGAGCT CATGTTTGAC 
AATGCGCTGA GTGCCCTTCG GCCAGAAGAG ATTGCTGCTC TGCTATCTGG CCTTGTCTGC 
CAAAGTCCTG GAGATCCTGG AGACCAATTG CCAAGCACTC TTAAACAGGG CATAGAGCGT 
GTCAAGGATG TGGCCCGGCG GATTGGTGAA GTTCAAGTAT CCTGTGGCCT AAACCAGACT 
GTGGAAGAGT TTGTGGGAGA ACTACACTTT GGGCTGGTAG AAGTTGTCTA TGAGTGGGCA 
CGAGGCATGC CTTTCTCTGA GCTGGCAGGG CTATCAGGGA CCCCAGAAGG TTTGATTGTC 
AGATGTATCC AGCGCCTGGC AGAGATGTGT CGTTCATTGC GAGGGGCTGC CCGGCTGGTG 
GGAGAACCTG TGCTAGGTGC TAAGATGGAG GCAGCTGCCA CAATGCTCCG TCGTGACATA 
GTTTTTGCTG CTAGTCTCTA TACCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIV2L ( XM_003768913.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003768961.1 Sarcophilus harrisii superkiller viralicidic activity 2-like (S. cerevisiae) (SKIV2L), mRNA.

Price & Availability↑

CloneID	OSa131050
Clone ID Related Accession (Same CDS sequence)	XM_003768913.3 , XM_003768913.2 , XM_003768913.4
Accession Version	XM_003768913.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	helicase SKI2W
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003768913.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGA CGGAGCGTCT GGTACTGCCC CCTCGGGGGC CCCTGGATTT GCCTTTGTGT 
GCGCTGGAGC TCGGATGCAC TGGGCGCTGG GAGCTTTTGA GCCAGCCTCA GGTCTCGGAT 
TCGCCGGCGG GCACCCTCTC TCATGGGCTA CCACCCTGTG CCCCAGAACT GCAAGGAGAG 
ACTGAGCAAT TGTTTCTGTC ATCCCCTGAA TGGCTGCCCC TGCATGGTGT GGAACGTGTG 
GCCTGGAAAT GGCAGAGAAA GACAGATCCC TGGTCTCTCC TGGGGCTGCC AGGAGCACCT 
GTCCCATCAG ATCTGCAGGC TCAAAGACAC CCAACCACCG GCAGAGTCCT GGGCTACAGA 
GAGGTCCTGC TGGAGGACAC AAACCTTTCG GCCACAACTT CCCTGTCTCT ACGCCGGCCC 
CCAGGACCCT CATCCCAGGC TTTATGGGGC AACCCAACAC ACTATCCATT TTGGCCAGGT 
GGGATGGATG AACCTACCAT AGCAGATCTG AGCATTTGTG GGGACTCTGA GGAAGAGATT 
GACTTTGAGA AAGATCTCCT TACCATTCCC CCAGGCTTCA AGAAAGGTGT AGGCTTCACT 
CCAAAGAATA ACTCAACCCC TGCTTCTGGC CTTCTCAGTC TTAGCCATCT TCTCGAACCC 
CTGGAACTTG GTGGAGGGGA GGAAGAGGAA GAAGAGACTT CAGGAAGATT GGGGGGTCCT 
GGAGGGGACA GTGTTACAGC CTCCACTTCT GAGCCCCCAC TGGTTCATGC TAGTAGCCTG 
GAGGATTTAG TCCTAAAGGA TGCATCAGCC CCCTTGTCAC CTCCAGAACC TCCAAAGCCC 
CCAGTCTTGG AACAGTGGGC TATTCCTGTG GATATTACTT CACCTGTGGG AGATTTCTAC 
CGGATTATCC CCCAACCTGC CTTCCAGTGG CCGTTTGAAC CAGATGTATT TCAGAAACAG 
GCTATTCTGC ACCTGGAGAG ACATGACTCT GTATTTGTTG CTGCTCACAC ATCTGCAGGA 
AAGACTGTTG TGGCTGAATA TGCCATTGCC CTTGCCCAGA AACATATGAC CCGTACCATC 
TATACATCAC CTATCAAGGC CCTGAGTAAT CAGAAATTTA GAGATTTCCG AAACACCTTT 
GGTGATGTGG GACTCCTCAC AGGGGATGTC CAGCTACATC CTGAAGCTTC CTGCCTCATT 
ATGACCACAG AGATCCTTCG CTCCATGCTG TATAGTGGTT CAGATGTGAT TCGAGACCTT 
GAATGGGTGA TATTTGATGA GGTCCATTAC ATCAATGATG CTGAGAGAGG AGTAGTGTGG 
GAAGAAGTCC TCATCATGCT TCCTGATCAT GTTTCCATCA TTCTCCTCAG TGCTACTGTG 
CCCAATGCCC TTGAATTTGC AGATTGGATT GGGAGGCTGA AGCGTCGGCA TCTCTATGTG 
ATCAGCACAG CTGCCCGACC AGTTCCCTTG GAACATTTTC TATTCACTGG GAATAGTCCA 
AAAACCCAAG GAGAGCTTTT TCTGCTTCTG GATTCACGAG GAGTTTTTCA CACTAAGGGA 
TACTATGCAG CTGTGGAGGC CAAGAAAGAA AGGACTAGCA AACATGCACA GACCTTTGGG 
GCAAAGCAGC CAATGCACCA AGGTGGGCCT GGACAGGACC GAGGCATATA CCTGTCCCTC 
TTGGCCTCCC TGCGGAGCCG TGCCCAGCTC CCTGTGGTGG TGTTCACCTT CTCCCGGGGC 
CGCTGTGATG AGCAGGCGTC AGGTCTCAGC TCCCTTGACC TTACCACCAG CTCTGAGAAG 
AGTGAGATCC ACCTTTTCCT GCAGCGATGC CTTGCACGAC TTCGAGGCTC CGACCGCCAG 
CTACCTCAGG TACTGCATAT GTCAGAACTC CTTCGCCGGG GTCTAGGTGT ACACCACAGC 
GGTATATTGC CCATTCTCAA GGAGATTGTG GAAATGCTCT TCAGTCGTGG TTTGGTCAAG 
GTCCTATTTG CTACAGAAAC CTTTGCCATG GGTGTGAACA TGCCAGCACG GACTGTGGTG 
TTTGATTCTA TGCGCAAGCA TGATGGTGCA ACTTTCCGTG ACTTGCTCCC TGGGGAATAT 
GTACAGATGG CAGGGCGGGC AGGGCGTCGT GGTCTGGACC CTACAGGCAC TGTCATCCTA 
CTCTGTAAGG GACGTGTGCC TGAGATGGCT GATCTGCATA GGATGATGTT GGGAAAGCCT 
TCCCAGCTAC AGTCCCAATT TCGCCTCACA TATACAATGA TTCTTAACTT GCTTCGGGTT 
GATGCCCTCA GAGTGGAAGA TATGATGAAG AGAAGCTTTT CTGAGTTCCC TTCAAGAAAG 
GACAGCAAGG CACATGAGCA GACTTTGGCA GAATTGACAA AGAGACTTGG GGACCTAGAG 
GAACCAGATA CCTCAGGGCA GCTTGCTGAT CTTCCTGAGT ATTACAGCTG GGGGGAAGAG 
CTGACAGAGA CTCGGGGCCT AATCCAGCGA CGGATCATGG AATCAGTGAA TGGGTTGAAA 
TCTCTGTCAG TGGGAAGAGT GGTAGTGGTT AAGAATCAGG AGCATCGGAA TGCACTTGGA 
GTGATCTTGC AGGTCTCCTC AGATACCACA AACCGAGTTT TCACAACCTT GATTCTCTGT 
GAGAAGCAGC CCCCTGAGGG GCCACTGCCT GGCAGTTCCA GATCCCTTGA TGTTCCCTAT 
CCTGATGATT TAGTGGGTCA CAAGCTATTC CTCCCTGAAG GGCCTTGTGA TCATACTGTG 
GCAAAACTTC AGCCAAGTGA CATCATTGCT ATCACAACAA AGATGCTACG GGTGAATGGG 
GAACGAATTT TGGAAGATTT TAGCAAGAGG CAGCAGCCAA GATTCAAGAA GGACCCTCCC 
TCTCCATTGG TGACCTCTGC TGTTCAGGAG ATGCTCCGAT TGGCTCAGGC ACATCCAGCG 
GGACCCCCCA CTCTGGATCC AGTCAATGAT TTACAACTGA AGGAGGTGTT AGTAGTTGAG 
GGTGGGCTCC GTGCTCGGAA GCTAGAAGAA TTGATTCGAG GTGCCCAGTG TGTACATAGT 
CCCAGGTTTC CAGCCCAGTA CCTGAGACTG CAGGAACGTG TGCAGATCCA GAAGGAGATG 
GAAAGATTAC GGTTTCTACT TTCAGACCAG TCATTGCTCC TGCTGCCAGA GTATCATCAG 
CGAGTGGAGA TCCTGAGGAC ATTGGGCTAT GTGGATGGGG CAGGGACCGT GAAGCTGGCA 
GGAAGGGTGG CATGTGCCAT GAGTAGTCAT GAACTGCTTC TGACAGAGCT CATGTTTGAC 
AATGCGCTGA GTGCCCTTCG GCCAGAAGAG ATTGCTGCTC TGCTATCTGG CCTTGTCTGC 
CAAAGTCCTG GAGATCCTGG AGACCAATTG CCAAGCACTC TTAAACAGGG CATAGAGCGT 
GTCAAGGATG TGGCCCGGCG GATTGGTGAA GTTCAAGTAT CCTGTGGCCT AAACCAGACT 
GTGGAAGAGT TTGTGGGAGA ACTACACTTT GGGCTGGTAG AAGTTGTCTA TGAGTGGGCA 
CGAGGCATGC CTTTCTCTGA GCTGGCAGGG CTATCAGGGA CCCCAGAAGG TTTGATTGTC 
AGATGTATCC AGCGCCTGGC AGAGATGTGT CGTTCATTGC GAGGGGCTGC CCGGCTGGTG 
GGAGAACCTG TGCTAGGTGC TAAGATGGAG GCAGCTGCCA CAATGCTCCG TCGTGACATA 
GTTTTTGCTG CTAGTCTCTA TACCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003768961.1
CDS	18..3767
Translation	MLETERLVLPPRGPLDLPLCALELGCTGRWELLSQPQVSDSPAGTLSHGLPPCAPELQGETEQLFLSSPEWLPLHGVERVAWKWQRKTDPWSLLGLPGAPVPSDLQAQRHPTTGRVLGYREVLLEDTNLSATTSLSLRRPPGPSSQALWGNPTHYPFWPGGMDEPTIADLSICGDSEEEIDFEKDLLTIPPGFKKGVGFTPKNNSTPASGLLSLSHLLEPLELGGGEEEEEETSGRLGGPGGDSVTASTSEPPLVHASSLEDLVLKDASAPLSPPEPPKPPVLEQWAIPVDITSPVGDFYRIIPQPAFQWPFEPDVFQKQAILHLERHDSVFVAAHTSAGKTVVAEYAIALAQKHMTRTIYTSPIKALSNQKFRDFRNTFGDVGLLTGDVQLHPEASCLIMTTEILRSMLYSGSDVIRDLEWVIFDEVHYINDAERGVVWEEVLIMLPDHVSIILLSATVPNALEFADWIGRLKRRHLYVISTAARPVPLEHFLFTGNSPKTQGELFLLLDSRGVFHTKGYYAAVEAKKERTSKHAQTFGAKQPMHQGGPGQDRGIYLSLLASLRSRAQLPVVVFTFSRGRCDEQASGLSSLDLTTSSEKSEIHLFLQRCLARLRGSDRQLPQVLHMSELLRRGLGVHHSGILPILKEIVEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSMRKHDGATFRDLLPGEYVQMAGRAGRRGLDPTGTVILLCKGRVPEMADLHRMMLGKPSQLQSQFRLTYTMILNLLRVDALRVEDMMKRSFSEFPSRKDSKAHEQTLAELTKRLGDLEEPDTSGQLADLPEYYSWGEELTETRGLIQRRIMESVNGLKSLSVGRVVVVKNQEHRNALGVILQVSSDTTNRVFTTLILCEKQPPEGPLPGSSRSLDVPYPDDLVGHKLFLPEGPCDHTVAKLQPSDIIAITTKMLRVNGERILEDFSKRQQPRFKKDPPSPLVTSAVQEMLRLAQAHPAGPPTLDPVNDLQLKEVLVVEGGLRARKLEELIRGAQCVHSPRFPAQYLRLQERVQIQKEMERLRFLLSDQSLLLLPEYHQRVEILRTLGYVDGAGTVKLAGRVACAMSSHELLLTELMFDNALSALRPEEIAALLSGLVCQSPGDPGDQLPSTLKQGIERVKDVARRIGEVQVSCGLNQTVEEFVGELHFGLVEVVYEWARGMPFSELAGLSGTPEGLIVRCIQRLAEMCRSLRGAARLVGEPVLGAKMEAAATMLRRDIVFAASLYTQ

Target ORF information:

RefSeq Version	XM_003768913.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii superkiller viralicidic activity 2-like (S. cerevisiae) (SKIV2L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003768913.2

ORF Insert Sequence:

ATGCTGGAGA CGGAGCGTCT GGTACTGCCC CCTCGGGGGC CCCTGGATTT GCCTTTGTGT 
GCGCTGGAGC TCGGATGCAC TGGGCGCTGG GAGCTTTTGA GCCAGCCTCA GGTCTCGGAT 
TCGCCGGCGG GCACCCTCTC TCATGGGCTA CCACCCTGTG CCCCAGAACT GCAAGGAGAG 
ACTGAGCAAT TGTTTCTGTC ATCCCCTGAA TGGCTGCCCC TGCATGGTGT GGAACGTGTG 
GCCTGGAAAT GGCAGAGAAA GACAGATCCC TGGTCTCTCC TGGGGCTGCC AGGAGCACCT 
GTCCCATCAG ATCTGCAGGC TCAAAGACAC CCAACCACCG GCAGAGTCCT GGGCTACAGA 
GAGGTCCTGC TGGAGGACAC AAACCTTTCG GCCACAACTT CCCTGTCTCT ACGCCGGCCC 
CCAGGACCCT CATCCCAGGC TTTATGGGGC AACCCAACAC ACTATCCATT TTGGCCAGGT 
GGGATGGATG AACCTACCAT AGCAGATCTG AGCATTTGTG GGGACTCTGA GGAAGAGATT 
GACTTTGAGA AAGATCTCCT TACCATTCCC CCAGGCTTCA AGAAAGGTGT AGGCTTCACT 
CCAAAGAATA ACTCAACCCC TGCTTCTGGC CTTCTCAGTC TTAGCCATCT TCTCGAACCC 
CTGGAACTTG GTGGAGGGGA GGAAGAGGAA GAAGAGACTT CAGGAAGATT GGGGGGTCCT 
GGAGGGGACA GTGTTACAGC CTCCACTTCT GAGCCCCCAC TGGTTCATGC TAGTAGCCTG 
GAGGATTTAG TCCTAAAGGA TGCATCAGCC CCCTTGTCAC CTCCAGAACC TCCAAAGCCC 
CCAGTCTTGG AACAGTGGGC TATTCCTGTG GATATTACTT CACCTGTGGG AGATTTCTAC 
CGGATTATCC CCCAACCTGC CTTCCAGTGG CCGTTTGAAC CAGATGTATT TCAGAAACAG 
GCTATTCTGC ACCTGGAGAG ACATGACTCT GTATTTGTTG CTGCTCACAC ATCTGCAGGA 
AAGACTGTTG TGGCTGAATA TGCCATTGCC CTTGCCCAGA AACATATGAC CCGTACCATC 
TATACATCAC CTATCAAGGC CCTGAGTAAT CAGAAATTTA GAGATTTCCG AAACACCTTT 
GGTGATGTGG GACTCCTCAC AGGGGATGTC CAGCTACATC CTGAAGCTTC CTGCCTCATT 
ATGACCACAG AGATCCTTCG CTCCATGCTG TATAGTGGTT CAGATGTGAT TCGAGACCTT 
GAATGGGTGA TATTTGATGA GGTCCATTAC ATCAATGATG CTGAGAGAGG AGTAGTGTGG 
GAAGAAGTCC TCATCATGCT TCCTGATCAT GTTTCCATCA TTCTCCTCAG TGCTACTGTG 
CCCAATGCCC TTGAATTTGC AGATTGGATT GGGAGGCTGA AGCGTCGGCA TCTCTATGTG 
ATCAGCACAG CTGCCCGACC AGTTCCCTTG GAACATTTTC TATTCACTGG GAATAGTCCA 
AAAACCCAAG GAGAGCTTTT TCTGCTTCTG GATTCACGAG GAGTTTTTCA CACTAAGGGA 
TACTATGCAG CTGTGGAGGC CAAGAAAGAA AGGACTAGCA AACATGCACA GACCTTTGGG 
GCAAAGCAGC CAATGCACCA AGGTGGGCCT GGACAGGACC GAGGCATATA CCTGTCCCTC 
TTGGCCTCCC TGCGGAGCCG TGCCCAGCTC CCTGTGGTGG TGTTCACCTT CTCCCGGGGC 
CGCTGTGATG AGCAGGCGTC AGGTCTCAGC TCCCTTGACC TTACCACCAG CTCTGAGAAG 
AGTGAGATCC ACCTTTTCCT GCAGCGATGC CTTGCACGAC TTCGAGGCTC CGACCGCCAG 
CTACCTCAGG TACTGCATAT GTCAGAACTC CTTCGCCGGG GTCTAGGTGT ACACCACAGC 
GGTATATTGC CCATTCTCAA GGAGATTGTG GAAATGCTCT TCAGTCGTGG TTTGGTCAAG 
GTCCTATTTG CTACAGAAAC CTTTGCCATG GGTGTGAACA TGCCAGCACG GACTGTGGTG 
TTTGATTCTA TGCGCAAGCA TGATGGTGCA ACTTTCCGTG ACTTGCTCCC TGGGGAATAT 
GTACAGATGG CAGGGCGGGC AGGGCGTCGT GGTCTGGACC CTACAGGCAC TGTCATCCTA 
CTCTGTAAGG GACGTGTGCC TGAGATGGCT GATCTGCATA GGATGATGTT GGGAAAGCCT 
TCCCAGCTAC AGTCCCAATT TCGCCTCACA TATACAATGA TTCTTAACTT GCTTCGGGTT 
GATGCCCTCA GAGTGGAAGA TATGATGAAG AGAAGCTTTT CTGAGTTCCC TTCAAGAAAG 
GACAGCAAGG CACATGAGCA GACTTTGGCA GAATTGACAA AGAGACTTGG GGACCTAGAG 
GAACCAGATA CCTCAGGGCA GCTTGCTGAT CTTCCTGAGT ATTACAGCTG GGGGGAAGAG 
CTGACAGAGA CTCGGGGCCT AATCCAGCGA CGGATCATGG AATCAGTGAA TGGGTTGAAA 
TCTCTGTCAG TGGGAAGAGT GGTAGTGGTT AAGAATCAGG AGCATCGGAA TGCACTTGGA 
GTGATCTTGC AGGTCTCCTC AGATACCACA AACCGAGTTT TCACAACCTT GATTCTCTGT 
GAGAAGCAGC CCCCTGAGGG GCCACTGCCT GGCAGTTCCA GATCCCTTGA TGTTCCCTAT 
CCTGATGATT TAGTGGGTCA CAAGCTATTC CTCCCTGAAG GGCCTTGTGA TCATACTGTG 
GCAAAACTTC AGCCAAGTGA CATCATTGCT ATCACAACAA AGATGCTACG GGTGAATGGG 
GAACGAATTT TGGAAGATTT TAGCAAGAGG CAGCAGCCAA GATTCAAGAA GGACCCTCCC 
TCTCCATTGG TGACCTCTGC TGTTCAGGAG ATGCTCCGAT TGGCTCAGGC ACATCCAGCG 
GGACCCCCCA CTCTGGATCC AGTCAATGAT TTACAACTGA AGGAGGTGTT AGTAGTTGAG 
GGTGGGCTCC GTGCTCGGAA GCTAGAAGAA TTGATTCGAG GTGCCCAGTG TGTACATAGT 
CCCAGGTTTC CAGCCCAGTA CCTGAGACTG CAGGAACGTG TGCAGATCCA GAAGGAGATG 
GAAAGATTAC GGTTTCTACT TTCAGACCAG TCATTGCTCC TGCTGCCAGA GTATCATCAG 
CGAGTGGAGA TCCTGAGGAC ATTGGGCTAT GTGGATGGGG CAGGGACCGT GAAGCTGGCA 
GGAAGGGTGG CATGTGCCAT GAGTAGTCAT GAACTGCTTC TGACAGAGCT CATGTTTGAC 
AATGCGCTGA GTGCCCTTCG GCCAGAAGAG ATTGCTGCTC TGCTATCTGG CCTTGTCTGC 
CAAAGTCCTG GAGATCCTGG AGACCAATTG CCAAGCACTC TTAAACAGGG CATAGAGCGT 
GTCAAGGATG TGGCCCGGCG GATTGGTGAA GTTCAAGTAT CCTGTGGCCT AAACCAGACT 
GTGGAAGAGT TTGTGGGAGA ACTACACTTT GGGCTGGTAG AAGTTGTCTA TGAGTGGGCA 
CGAGGCATGC CTTTCTCTGA GCTGGCAGGG CTATCAGGGA CCCCAGAAGG TTTGATTGTC 
AGATGTATCC AGCGCCTGGC AGAGATGTGT CGTTCATTGC GAGGGGCTGC CCGGCTGGTG 
GGAGAACCTG TGCTAGGTGC TAAGATGGAG GCAGCTGCCA CAATGCTCCG TCGTGACATA 
GTTTTTGCTG CTAGTCTCTA TACCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIV2L ( XM_003768913.4 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003768961.1 Sarcophilus harrisii Ski2 like RNA helicase (SKIV2L), transcript variant X1, mRNA.

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CloneID	OSa131050
Clone ID Related Accession (Same CDS sequence)	XM_003768913.3 , XM_003768913.2 , XM_003768913.4
Accession Version	XM_003768913.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	helicase SKI2W isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_003768913.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGA CGGAGCGTCT GGTACTGCCC CCTCGGGGGC CCCTGGATTT GCCTTTGTGT 
GCGCTGGAGC TCGGATGCAC TGGGCGCTGG GAGCTTTTGA GCCAGCCTCA GGTCTCGGAT 
TCGCCGGCGG GCACCCTCTC TCATGGGCTA CCACCCTGTG CCCCAGAACT GCAAGGAGAG 
ACTGAGCAAT TGTTTCTGTC ATCCCCTGAA TGGCTGCCCC TGCATGGTGT GGAACGTGTG 
GCCTGGAAAT GGCAGAGAAA GACAGATCCC TGGTCTCTCC TGGGGCTGCC AGGAGCACCT 
GTCCCATCAG ATCTGCAGGC TCAAAGACAC CCAACCACCG GCAGAGTCCT GGGCTACAGA 
GAGGTCCTGC TGGAGGACAC AAACCTTTCG GCCACAACTT CCCTGTCTCT ACGCCGGCCC 
CCAGGACCCT CATCCCAGGC TTTATGGGGC AACCCAACAC ACTATCCATT TTGGCCAGGT 
GGGATGGATG AACCTACCAT AGCAGATCTG AGCATTTGTG GGGACTCTGA GGAAGAGATT 
GACTTTGAGA AAGATCTCCT TACCATTCCC CCAGGCTTCA AGAAAGGTGT AGGCTTCACT 
CCAAAGAATA ACTCAACCCC TGCTTCTGGC CTTCTCAGTC TTAGCCATCT TCTCGAACCC 
CTGGAACTTG GTGGAGGGGA GGAAGAGGAA GAAGAGACTT CAGGAAGATT GGGGGGTCCT 
GGAGGGGACA GTGTTACAGC CTCCACTTCT GAGCCCCCAC TGGTTCATGC TAGTAGCCTG 
GAGGATTTAG TCCTAAAGGA TGCATCAGCC CCCTTGTCAC CTCCAGAACC TCCAAAGCCC 
CCAGTCTTGG AACAGTGGGC TATTCCTGTG GATATTACTT CACCTGTGGG AGATTTCTAC 
CGGATTATCC CCCAACCTGC CTTCCAGTGG CCGTTTGAAC CAGATGTATT TCAGAAACAG 
GCTATTCTGC ACCTGGAGAG ACATGACTCT GTATTTGTTG CTGCTCACAC ATCTGCAGGA 
AAGACTGTTG TGGCTGAATA TGCCATTGCC CTTGCCCAGA AACATATGAC CCGTACCATC 
TATACATCAC CTATCAAGGC CCTGAGTAAT CAGAAATTTA GAGATTTCCG AAACACCTTT 
GGTGATGTGG GACTCCTCAC AGGGGATGTC CAGCTACATC CTGAAGCTTC CTGCCTCATT 
ATGACCACAG AGATCCTTCG CTCCATGCTG TATAGTGGTT CAGATGTGAT TCGAGACCTT 
GAATGGGTGA TATTTGATGA GGTCCATTAC ATCAATGATG CTGAGAGAGG AGTAGTGTGG 
GAAGAAGTCC TCATCATGCT TCCTGATCAT GTTTCCATCA TTCTCCTCAG TGCTACTGTG 
CCCAATGCCC TTGAATTTGC AGATTGGATT GGGAGGCTGA AGCGTCGGCA TCTCTATGTG 
ATCAGCACAG CTGCCCGACC AGTTCCCTTG GAACATTTTC TATTCACTGG GAATAGTCCA 
AAAACCCAAG GAGAGCTTTT TCTGCTTCTG GATTCACGAG GAGTTTTTCA CACTAAGGGA 
TACTATGCAG CTGTGGAGGC CAAGAAAGAA AGGACTAGCA AACATGCACA GACCTTTGGG 
GCAAAGCAGC CAATGCACCA AGGTGGGCCT GGACAGGACC GAGGCATATA CCTGTCCCTC 
TTGGCCTCCC TGCGGAGCCG TGCCCAGCTC CCTGTGGTGG TGTTCACCTT CTCCCGGGGC 
CGCTGTGATG AGCAGGCGTC AGGTCTCAGC TCCCTTGACC TTACCACCAG CTCTGAGAAG 
AGTGAGATCC ACCTTTTCCT GCAGCGATGC CTTGCACGAC TTCGAGGCTC CGACCGCCAG 
CTACCTCAGG TACTGCATAT GTCAGAACTC CTTCGCCGGG GTCTAGGTGT ACACCACAGC 
GGTATATTGC CCATTCTCAA GGAGATTGTG GAAATGCTCT TCAGTCGTGG TTTGGTCAAG 
GTCCTATTTG CTACAGAAAC CTTTGCCATG GGTGTGAACA TGCCAGCACG GACTGTGGTG 
TTTGATTCTA TGCGCAAGCA TGATGGTGCA ACTTTCCGTG ACTTGCTCCC TGGGGAATAT 
GTACAGATGG CAGGGCGGGC AGGGCGTCGT GGTCTGGACC CTACAGGCAC TGTCATCCTA 
CTCTGTAAGG GACGTGTGCC TGAGATGGCT GATCTGCATA GGATGATGTT GGGAAAGCCT 
TCCCAGCTAC AGTCCCAATT TCGCCTCACA TATACAATGA TTCTTAACTT GCTTCGGGTT 
GATGCCCTCA GAGTGGAAGA TATGATGAAG AGAAGCTTTT CTGAGTTCCC TTCAAGAAAG 
GACAGCAAGG CACATGAGCA GACTTTGGCA GAATTGACAA AGAGACTTGG GGACCTAGAG 
GAACCAGATA CCTCAGGGCA GCTTGCTGAT CTTCCTGAGT ATTACAGCTG GGGGGAAGAG 
CTGACAGAGA CTCGGGGCCT AATCCAGCGA CGGATCATGG AATCAGTGAA TGGGTTGAAA 
TCTCTGTCAG TGGGAAGAGT GGTAGTGGTT AAGAATCAGG AGCATCGGAA TGCACTTGGA 
GTGATCTTGC AGGTCTCCTC AGATACCACA AACCGAGTTT TCACAACCTT GATTCTCTGT 
GAGAAGCAGC CCCCTGAGGG GCCACTGCCT GGCAGTTCCA GATCCCTTGA TGTTCCCTAT 
CCTGATGATT TAGTGGGTCA CAAGCTATTC CTCCCTGAAG GGCCTTGTGA TCATACTGTG 
GCAAAACTTC AGCCAAGTGA CATCATTGCT ATCACAACAA AGATGCTACG GGTGAATGGG 
GAACGAATTT TGGAAGATTT TAGCAAGAGG CAGCAGCCAA GATTCAAGAA GGACCCTCCC 
TCTCCATTGG TGACCTCTGC TGTTCAGGAG ATGCTCCGAT TGGCTCAGGC ACATCCAGCG 
GGACCCCCCA CTCTGGATCC AGTCAATGAT TTACAACTGA AGGAGGTGTT AGTAGTTGAG 
GGTGGGCTCC GTGCTCGGAA GCTAGAAGAA TTGATTCGAG GTGCCCAGTG TGTACATAGT 
CCCAGGTTTC CAGCCCAGTA CCTGAGACTG CAGGAACGTG TGCAGATCCA GAAGGAGATG 
GAAAGATTAC GGTTTCTACT TTCAGACCAG TCATTGCTCC TGCTGCCAGA GTATCATCAG 
CGAGTGGAGA TCCTGAGGAC ATTGGGCTAT GTGGATGGGG CAGGGACCGT GAAGCTGGCA 
GGAAGGGTGG CATGTGCCAT GAGTAGTCAT GAACTGCTTC TGACAGAGCT CATGTTTGAC 
AATGCGCTGA GTGCCCTTCG GCCAGAAGAG ATTGCTGCTC TGCTATCTGG CCTTGTCTGC 
CAAAGTCCTG GAGATCCTGG AGACCAATTG CCAAGCACTC TTAAACAGGG CATAGAGCGT 
GTCAAGGATG TGGCCCGGCG GATTGGTGAA GTTCAAGTAT CCTGTGGCCT AAACCAGACT 
GTGGAAGAGT TTGTGGGAGA ACTACACTTT GGGCTGGTAG AAGTTGTCTA TGAGTGGGCA 
CGAGGCATGC CTTTCTCTGA GCTGGCAGGG CTATCAGGGA CCCCAGAAGG TTTGATTGTC 
AGATGTATCC AGCGCCTGGC AGAGATGTGT CGTTCATTGC GAGGGGCTGC CCGGCTGGTG 
GGAGAACCTG TGCTAGGTGC TAAGATGGAG GCAGCTGCCA CAATGCTCCG TCGTGACATA 
GTTTTTGCTG CTAGTCTCTA TACCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003768961.1
CDS	85..3834
Translation	MLETERLVLPPRGPLDLPLCALELGCTGRWELLSQPQVSDSPAGTLSHGLPPCAPELQGETEQLFLSSPEWLPLHGVERVAWKWQRKTDPWSLLGLPGAPVPSDLQAQRHPTTGRVLGYREVLLEDTNLSATTSLSLRRPPGPSSQALWGNPTHYPFWPGGMDEPTIADLSICGDSEEEIDFEKDLLTIPPGFKKGVGFTPKNNSTPASGLLSLSHLLEPLELGGGEEEEEETSGRLGGPGGDSVTASTSEPPLVHASSLEDLVLKDASAPLSPPEPPKPPVLEQWAIPVDITSPVGDFYRIIPQPAFQWPFEPDVFQKQAILHLERHDSVFVAAHTSAGKTVVAEYAIALAQKHMTRTIYTSPIKALSNQKFRDFRNTFGDVGLLTGDVQLHPEASCLIMTTEILRSMLYSGSDVIRDLEWVIFDEVHYINDAERGVVWEEVLIMLPDHVSIILLSATVPNALEFADWIGRLKRRHLYVISTAARPVPLEHFLFTGNSPKTQGELFLLLDSRGVFHTKGYYAAVEAKKERTSKHAQTFGAKQPMHQGGPGQDRGIYLSLLASLRSRAQLPVVVFTFSRGRCDEQASGLSSLDLTTSSEKSEIHLFLQRCLARLRGSDRQLPQVLHMSELLRRGLGVHHSGILPILKEIVEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSMRKHDGATFRDLLPGEYVQMAGRAGRRGLDPTGTVILLCKGRVPEMADLHRMMLGKPSQLQSQFRLTYTMILNLLRVDALRVEDMMKRSFSEFPSRKDSKAHEQTLAELTKRLGDLEEPDTSGQLADLPEYYSWGEELTETRGLIQRRIMESVNGLKSLSVGRVVVVKNQEHRNALGVILQVSSDTTNRVFTTLILCEKQPPEGPLPGSSRSLDVPYPDDLVGHKLFLPEGPCDHTVAKLQPSDIIAITTKMLRVNGERILEDFSKRQQPRFKKDPPSPLVTSAVQEMLRLAQAHPAGPPTLDPVNDLQLKEVLVVEGGLRARKLEELIRGAQCVHSPRFPAQYLRLQERVQIQKEMERLRFLLSDQSLLLLPEYHQRVEILRTLGYVDGAGTVKLAGRVACAMSSHELLLTELMFDNALSALRPEEIAALLSGLVCQSPGDPGDQLPSTLKQGIERVKDVARRIGEVQVSCGLNQTVEEFVGELHFGLVEVVYEWARGMPFSELAGLSGTPEGLIVRCIQRLAEMCRSLRGAARLVGEPVLGAKMEAAATMLRRDIVFAASLYTQ

Target ORF information:

RefSeq Version	XM_003768913.4
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Ski2 like RNA helicase (SKIV2L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003768913.4

ORF Insert Sequence:

ATGCTGGAGA CGGAGCGTCT GGTACTGCCC CCTCGGGGGC CCCTGGATTT GCCTTTGTGT 
GCGCTGGAGC TCGGATGCAC TGGGCGCTGG GAGCTTTTGA GCCAGCCTCA GGTCTCGGAT 
TCGCCGGCGG GCACCCTCTC TCATGGGCTA CCACCCTGTG CCCCAGAACT GCAAGGAGAG 
ACTGAGCAAT TGTTTCTGTC ATCCCCTGAA TGGCTGCCCC TGCATGGTGT GGAACGTGTG 
GCCTGGAAAT GGCAGAGAAA GACAGATCCC TGGTCTCTCC TGGGGCTGCC AGGAGCACCT 
GTCCCATCAG ATCTGCAGGC TCAAAGACAC CCAACCACCG GCAGAGTCCT GGGCTACAGA 
GAGGTCCTGC TGGAGGACAC AAACCTTTCG GCCACAACTT CCCTGTCTCT ACGCCGGCCC 
CCAGGACCCT CATCCCAGGC TTTATGGGGC AACCCAACAC ACTATCCATT TTGGCCAGGT 
GGGATGGATG AACCTACCAT AGCAGATCTG AGCATTTGTG GGGACTCTGA GGAAGAGATT 
GACTTTGAGA AAGATCTCCT TACCATTCCC CCAGGCTTCA AGAAAGGTGT AGGCTTCACT 
CCAAAGAATA ACTCAACCCC TGCTTCTGGC CTTCTCAGTC TTAGCCATCT TCTCGAACCC 
CTGGAACTTG GTGGAGGGGA GGAAGAGGAA GAAGAGACTT CAGGAAGATT GGGGGGTCCT 
GGAGGGGACA GTGTTACAGC CTCCACTTCT GAGCCCCCAC TGGTTCATGC TAGTAGCCTG 
GAGGATTTAG TCCTAAAGGA TGCATCAGCC CCCTTGTCAC CTCCAGAACC TCCAAAGCCC 
CCAGTCTTGG AACAGTGGGC TATTCCTGTG GATATTACTT CACCTGTGGG AGATTTCTAC 
CGGATTATCC CCCAACCTGC CTTCCAGTGG CCGTTTGAAC CAGATGTATT TCAGAAACAG 
GCTATTCTGC ACCTGGAGAG ACATGACTCT GTATTTGTTG CTGCTCACAC ATCTGCAGGA 
AAGACTGTTG TGGCTGAATA TGCCATTGCC CTTGCCCAGA AACATATGAC CCGTACCATC 
TATACATCAC CTATCAAGGC CCTGAGTAAT CAGAAATTTA GAGATTTCCG AAACACCTTT 
GGTGATGTGG GACTCCTCAC AGGGGATGTC CAGCTACATC CTGAAGCTTC CTGCCTCATT 
ATGACCACAG AGATCCTTCG CTCCATGCTG TATAGTGGTT CAGATGTGAT TCGAGACCTT 
GAATGGGTGA TATTTGATGA GGTCCATTAC ATCAATGATG CTGAGAGAGG AGTAGTGTGG 
GAAGAAGTCC TCATCATGCT TCCTGATCAT GTTTCCATCA TTCTCCTCAG TGCTACTGTG 
CCCAATGCCC TTGAATTTGC AGATTGGATT GGGAGGCTGA AGCGTCGGCA TCTCTATGTG 
ATCAGCACAG CTGCCCGACC AGTTCCCTTG GAACATTTTC TATTCACTGG GAATAGTCCA 
AAAACCCAAG GAGAGCTTTT TCTGCTTCTG GATTCACGAG GAGTTTTTCA CACTAAGGGA 
TACTATGCAG CTGTGGAGGC CAAGAAAGAA AGGACTAGCA AACATGCACA GACCTTTGGG 
GCAAAGCAGC CAATGCACCA AGGTGGGCCT GGACAGGACC GAGGCATATA CCTGTCCCTC 
TTGGCCTCCC TGCGGAGCCG TGCCCAGCTC CCTGTGGTGG TGTTCACCTT CTCCCGGGGC 
CGCTGTGATG AGCAGGCGTC AGGTCTCAGC TCCCTTGACC TTACCACCAG CTCTGAGAAG 
AGTGAGATCC ACCTTTTCCT GCAGCGATGC CTTGCACGAC TTCGAGGCTC CGACCGCCAG 
CTACCTCAGG TACTGCATAT GTCAGAACTC CTTCGCCGGG GTCTAGGTGT ACACCACAGC 
GGTATATTGC CCATTCTCAA GGAGATTGTG GAAATGCTCT TCAGTCGTGG TTTGGTCAAG 
GTCCTATTTG CTACAGAAAC CTTTGCCATG GGTGTGAACA TGCCAGCACG GACTGTGGTG 
TTTGATTCTA TGCGCAAGCA TGATGGTGCA ACTTTCCGTG ACTTGCTCCC TGGGGAATAT 
GTACAGATGG CAGGGCGGGC AGGGCGTCGT GGTCTGGACC CTACAGGCAC TGTCATCCTA 
CTCTGTAAGG GACGTGTGCC TGAGATGGCT GATCTGCATA GGATGATGTT GGGAAAGCCT 
TCCCAGCTAC AGTCCCAATT TCGCCTCACA TATACAATGA TTCTTAACTT GCTTCGGGTT 
GATGCCCTCA GAGTGGAAGA TATGATGAAG AGAAGCTTTT CTGAGTTCCC TTCAAGAAAG 
GACAGCAAGG CACATGAGCA GACTTTGGCA GAATTGACAA AGAGACTTGG GGACCTAGAG 
GAACCAGATA CCTCAGGGCA GCTTGCTGAT CTTCCTGAGT ATTACAGCTG GGGGGAAGAG 
CTGACAGAGA CTCGGGGCCT AATCCAGCGA CGGATCATGG AATCAGTGAA TGGGTTGAAA 
TCTCTGTCAG TGGGAAGAGT GGTAGTGGTT AAGAATCAGG AGCATCGGAA TGCACTTGGA 
GTGATCTTGC AGGTCTCCTC AGATACCACA AACCGAGTTT TCACAACCTT GATTCTCTGT 
GAGAAGCAGC CCCCTGAGGG GCCACTGCCT GGCAGTTCCA GATCCCTTGA TGTTCCCTAT 
CCTGATGATT TAGTGGGTCA CAAGCTATTC CTCCCTGAAG GGCCTTGTGA TCATACTGTG 
GCAAAACTTC AGCCAAGTGA CATCATTGCT ATCACAACAA AGATGCTACG GGTGAATGGG 
GAACGAATTT TGGAAGATTT TAGCAAGAGG CAGCAGCCAA GATTCAAGAA GGACCCTCCC 
TCTCCATTGG TGACCTCTGC TGTTCAGGAG ATGCTCCGAT TGGCTCAGGC ACATCCAGCG 
GGACCCCCCA CTCTGGATCC AGTCAATGAT TTACAACTGA AGGAGGTGTT AGTAGTTGAG 
GGTGGGCTCC GTGCTCGGAA GCTAGAAGAA TTGATTCGAG GTGCCCAGTG TGTACATAGT 
CCCAGGTTTC CAGCCCAGTA CCTGAGACTG CAGGAACGTG TGCAGATCCA GAAGGAGATG 
GAAAGATTAC GGTTTCTACT TTCAGACCAG TCATTGCTCC TGCTGCCAGA GTATCATCAG 
CGAGTGGAGA TCCTGAGGAC ATTGGGCTAT GTGGATGGGG CAGGGACCGT GAAGCTGGCA 
GGAAGGGTGG CATGTGCCAT GAGTAGTCAT GAACTGCTTC TGACAGAGCT CATGTTTGAC 
AATGCGCTGA GTGCCCTTCG GCCAGAAGAG ATTGCTGCTC TGCTATCTGG CCTTGTCTGC 
CAAAGTCCTG GAGATCCTGG AGACCAATTG CCAAGCACTC TTAAACAGGG CATAGAGCGT 
GTCAAGGATG TGGCCCGGCG GATTGGTGAA GTTCAAGTAT CCTGTGGCCT AAACCAGACT 
GTGGAAGAGT TTGTGGGAGA ACTACACTTT GGGCTGGTAG AAGTTGTCTA TGAGTGGGCA 
CGAGGCATGC CTTTCTCTGA GCTGGCAGGG CTATCAGGGA CCCCAGAAGG TTTGATTGTC 
AGATGTATCC AGCGCCTGGC AGAGATGTGT CGTTCATTGC GAGGGGCTGC CCGGCTGGTG 
GGAGAACCTG TGCTAGGTGC TAAGATGGAG GCAGCTGCCA CAATGCTCCG TCGTGACATA 
GTTTTTGCTG CTAGTCTCTA TACCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIV2L ( XM_031965145.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031821005.1 Sarcophilus harrisii Ski2 like RNA helicase (SKIV2L), transcript variant X2, mRNA.

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CloneID	OSa233174
Clone ID Related Accession (Same CDS sequence)	XM_031965145.1
Accession Version	XM_031965145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	helicase SKI2W isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTTTA GGGGAGGGAT CTGGAACTGG ACGCGGCAAC TTCCGCCATG CCAGGGGCGG 
GACTTCCAGA GCTTCGGTCT TCTAAGCACT TCCGGCTTCG CGAAGACTGT GGGAAGCTGC 
GTTATGCTGG AGACGGAGCG TCTGGTACTG CCCCCTCGGG GGCCCCTGGA TTTGCCTTTG 
TGTGCGCTGG AGCTCGGATG CACTGGGCGC TGGGAGCTTT TGAGCCAGCC TCAGGTCTCG 
GATTCGCCGG CGGGCACCCT CTCTCATGGG CTACCACCCT GTGCCCCAGA ACTGCAAGGA 
GAGACTGAGC AATTGTTTCT GTCATCCCCT GAATGGCTGC CCCTGCATGG TGTGGAACGT 
GTGGCCTGGA AATGGCAGAG AAAGACAGAT CCCTGGTCTC TCCTGGGGCT GCCAGGAGCA 
CCTGTCCCAT CAGATCTGCA GGCTCAAAGA CACCCAACCA CCGGCAGAGT CCTGGGCTAC 
AGAGAGGTCC TGCTGGAGGA CACAAACCTT TCGGCCACAA CTTCCCTGTC TCTACGCCGG 
CCCCCAGGAC CCTCATCCCA GGCTTTATGG GGCAACCCAA CACACTATCC ATTTTGGCCA 
GGTGGGATGG ATGAACCTAC CATAGCAGAT CTGAGCATTT GTGGGGACTC TGAGGAAGAG 
ATTGACTTTG AGAAAGATCT CCTTACCATT CCCCCAGGCT TCAAGAAAGG TGTAGGCTTC 
ACTCCAAAGA ATAACTCAAC CCCTGCTTCT GGCCTTCTCA GTCTTAGCCA TCTTCTCGAA 
CCCCTGGAAC TTGGTGGAGG GGAGGAAGAG GAAGAAGAGA CTTCAGGAAG ATTGGGGGGT 
CCTGGAGGGG ACAGTGTTAC AGCCTCCACT TCTGAGCCCC CACTGGTTCA TGCTAGTAGC 
CTGGAGGATT TAGTCCTAAA GGATGCATCA GCCCCCTTGT CACCTCCAGA ACCTCCAAAG 
CCCCCAGTCT TGGAACAGTG GGCTATTCCT GTGGATATTA CTTCACCTGT GGGAGATTTC 
TACCGGATTA TCCCCCAACC TGCCTTCCAG TGGCCGTTTG AACCAGATGT ATTTCAGAAA 
CAGGCTATTC TGCACCTGGA GAGACATGAC TCTGTATTTG TTGCTGCTCA CACATCTGCA 
GGAAAGACTG TTGTGGCTGA ATATGCCATT GCCCTTGCCC AGAAACATAT GACCCGTACC 
ATCTATACAT CACCTATCAA GGCCCTGAGT AATCAGAAAT TTAGAGATTT CCGAAACACC 
TTTGGTGATG TGGGACTCCT CACAGGGGAT GTCCAGCTAC ATCCTGAAGC TTCCTGCCTC 
ATTATGACCA CAGAGATCCT TCGCTCCATG CTGTATAGTG GTTCAGATGT GATTCGAGAC 
CTTGAATGGG TGATATTTGA TGAGGTCCAT TACATCAATG ATGCTGAGAG AGGAGTAGTG 
TGGGAAGAAG TCCTCATCAT GCTTCCTGAT CATGTTTCCA TCATTCTCCT CAGTGCTACT 
GTGCCCAATG CCCTTGAATT TGCAGATTGG ATTGGGAGGC TGAAGCGTCG GCATCTCTAT 
GTGATCAGCA CAGCTGCCCG ACCAGTTCCC TTGGAACATT TTCTATTCAC TGGGAATAGT 
CCAAAAACCC AAGGAGAGCT TTTTCTGCTT CTGGATTCAC GAGGAGTTTT TCACACTAAG 
GGATACTATG CAGCTGTGGA GGCCAAGAAA GAAAGGACTA GCAAACATGC ACAGACCTTT 
GGGGCAAAGC AGCCAATGCA CCAAGGTGGG CCTGGACAGG ACCGAGGCAT ATACCTGTCC 
CTCTTGGCCT CCCTGCGGAG CCGTGCCCAG CTCCCTGTGG TGGTGTTCAC CTTCTCCCGG 
GGCCGCTGTG ATGAGCAGGC GTCAGGTCTC AGCTCCCTTG ACCTTACCAC CAGCTCTGAG 
AAGAGTGAGA TCCACCTTTT CCTGCAGCGA TGCCTTGCAC GACTTCGAGG CTCCGACCGC 
CAGCTACCTC AGGTACTGCA TATGTCAGAA CTCCTTCGCC GGGGTCTAGG TGTACACCAC 
AGCGGTATAT TGCCCATTCT CAAGGAGATT GTGGAAATGC TCTTCAGTCG TGGTTTGGTC 
AAGGTCCTAT TTGCTACAGA AACCTTTGCC ATGGGTGTGA ACATGCCAGC ACGGACTGTG 
GTGTTTGATT CTATGCGCAA GCATGATGGT GCAACTTTCC GTGACTTGCT CCCTGGGGAA 
TATGTACAGA TGGCAGGGCG GGCAGGGCGT CGTGGTCTGG ACCCTACAGG CACTGTCATC 
CTACTCTGTA AGGGACGTGT GCCTGAGATG GCTGATCTGC ATAGGATGAT GTTGGGAAAG 
CCTTCCCAGC TACAGTCCCA ATTTCGCCTC ACATATACAA TGATTCTTAA CTTGCTTCGG 
GTTGATGCCC TCAGAGTGGA AGATATGATG AAGAGAAGCT TTTCTGAGTT CCCTTCAAGA 
AAGGACAGCA AGGCACATGA GCAGACTTTG GCAGAATTGA CAAAGAGACT TGGGGACCTA 
GAGGAACCAG ATACCTCAGG GCAGCTTGCT GATCTTCCTG AGTATTACAG CTGGGGGGAA 
GAGCTGACAG AGACTCGGGG CCTAATCCAG CGACGGATCA TGGAATCAGT GAATGGGTTG 
AAATCTCTGT CAGTGGGAAG AGTGGTAGTG GTTAAGAATC AGGAGCATCG GAATGCACTT 
GGAGTGATCT TGCAGGTCTC CTCAGATACC ACAAACCGAG TTTTCACAAC CTTGATTCTC 
TGTGAGAAGC AGCCCCCTGA GGGGCCACTG CCTGGCAGTT CCAGATCCCT TGATGTTCCC 
TATCCTGATG ATTTAGTGGG TCACAAGCTA TTCCTCCCTG AAGGGCCTTG TGATCATACT 
GTGGCAAAAC TTCAGCCAAG TGACATCATT GCTATCACAA CAAAGATGCT ACGGGTGAAT 
GGGGAACGAA TTTTGGAAGA TTTTAGCAAG AGGCAGCAGC CAAGATTCAA GTTAGTAGCT 
TTGGGAAGGA CCCTCCCTCT CCATTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031821005.1
CDS	42..3071
Translation	MTFRGGIWNWTRQLPPCQGRDFQSFGLLSTSGFAKTVGSCVMLETERLVLPPRGPLDLPLCALELGCTGRWELLSQPQVSDSPAGTLSHGLPPCAPELQGETEQLFLSSPEWLPLHGVERVAWKWQRKTDPWSLLGLPGAPVPSDLQAQRHPTTGRVLGYREVLLEDTNLSATTSLSLRRPPGPSSQALWGNPTHYPFWPGGMDEPTIADLSICGDSEEEIDFEKDLLTIPPGFKKGVGFTPKNNSTPASGLLSLSHLLEPLELGGGEEEEEETSGRLGGPGGDSVTASTSEPPLVHASSLEDLVLKDASAPLSPPEPPKPPVLEQWAIPVDITSPVGDFYRIIPQPAFQWPFEPDVFQKQAILHLERHDSVFVAAHTSAGKTVVAEYAIALAQKHMTRTIYTSPIKALSNQKFRDFRNTFGDVGLLTGDVQLHPEASCLIMTTEILRSMLYSGSDVIRDLEWVIFDEVHYINDAERGVVWEEVLIMLPDHVSIILLSATVPNALEFADWIGRLKRRHLYVISTAARPVPLEHFLFTGNSPKTQGELFLLLDSRGVFHTKGYYAAVEAKKERTSKHAQTFGAKQPMHQGGPGQDRGIYLSLLASLRSRAQLPVVVFTFSRGRCDEQASGLSSLDLTTSSEKSEIHLFLQRCLARLRGSDRQLPQVLHMSELLRRGLGVHHSGILPILKEIVEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSMRKHDGATFRDLLPGEYVQMAGRAGRRGLDPTGTVILLCKGRVPEMADLHRMMLGKPSQLQSQFRLTYTMILNLLRVDALRVEDMMKRSFSEFPSRKDSKAHEQTLAELTKRLGDLEEPDTSGQLADLPEYYSWGEELTETRGLIQRRIMESVNGLKSLSVGRVVVVKNQEHRNALGVILQVSSDTTNRVFTTLILCEKQPPEGPLPGSSRSLDVPYPDDLVGHKLFLPEGPCDHTVAKLQPSDIIAITTKMLRVNGERILEDFSKRQQPRFKLVALGRTLPLHW

Target ORF information:

RefSeq Version	XM_031965145.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii Ski2 like RNA helicase (SKIV2L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031965145.1

ORF Insert Sequence:

ATGACGTTTA GGGGAGGGAT CTGGAACTGG ACGCGGCAAC TTCCGCCATG CCAGGGGCGG 
GACTTCCAGA GCTTCGGTCT TCTAAGCACT TCCGGCTTCG CGAAGACTGT GGGAAGCTGC 
GTTATGCTGG AGACGGAGCG TCTGGTACTG CCCCCTCGGG GGCCCCTGGA TTTGCCTTTG 
TGTGCGCTGG AGCTCGGATG CACTGGGCGC TGGGAGCTTT TGAGCCAGCC TCAGGTCTCG 
GATTCGCCGG CGGGCACCCT CTCTCATGGG CTACCACCCT GTGCCCCAGA ACTGCAAGGA 
GAGACTGAGC AATTGTTTCT GTCATCCCCT GAATGGCTGC CCCTGCATGG TGTGGAACGT 
GTGGCCTGGA AATGGCAGAG AAAGACAGAT CCCTGGTCTC TCCTGGGGCT GCCAGGAGCA 
CCTGTCCCAT CAGATCTGCA GGCTCAAAGA CACCCAACCA CCGGCAGAGT CCTGGGCTAC 
AGAGAGGTCC TGCTGGAGGA CACAAACCTT TCGGCCACAA CTTCCCTGTC TCTACGCCGG 
CCCCCAGGAC CCTCATCCCA GGCTTTATGG GGCAACCCAA CACACTATCC ATTTTGGCCA 
GGTGGGATGG ATGAACCTAC CATAGCAGAT CTGAGCATTT GTGGGGACTC TGAGGAAGAG 
ATTGACTTTG AGAAAGATCT CCTTACCATT CCCCCAGGCT TCAAGAAAGG TGTAGGCTTC 
ACTCCAAAGA ATAACTCAAC CCCTGCTTCT GGCCTTCTCA GTCTTAGCCA TCTTCTCGAA 
CCCCTGGAAC TTGGTGGAGG GGAGGAAGAG GAAGAAGAGA CTTCAGGAAG ATTGGGGGGT 
CCTGGAGGGG ACAGTGTTAC AGCCTCCACT TCTGAGCCCC CACTGGTTCA TGCTAGTAGC 
CTGGAGGATT TAGTCCTAAA GGATGCATCA GCCCCCTTGT CACCTCCAGA ACCTCCAAAG 
CCCCCAGTCT TGGAACAGTG GGCTATTCCT GTGGATATTA CTTCACCTGT GGGAGATTTC 
TACCGGATTA TCCCCCAACC TGCCTTCCAG TGGCCGTTTG AACCAGATGT ATTTCAGAAA 
CAGGCTATTC TGCACCTGGA GAGACATGAC TCTGTATTTG TTGCTGCTCA CACATCTGCA 
GGAAAGACTG TTGTGGCTGA ATATGCCATT GCCCTTGCCC AGAAACATAT GACCCGTACC 
ATCTATACAT CACCTATCAA GGCCCTGAGT AATCAGAAAT TTAGAGATTT CCGAAACACC 
TTTGGTGATG TGGGACTCCT CACAGGGGAT GTCCAGCTAC ATCCTGAAGC TTCCTGCCTC 
ATTATGACCA CAGAGATCCT TCGCTCCATG CTGTATAGTG GTTCAGATGT GATTCGAGAC 
CTTGAATGGG TGATATTTGA TGAGGTCCAT TACATCAATG ATGCTGAGAG AGGAGTAGTG 
TGGGAAGAAG TCCTCATCAT GCTTCCTGAT CATGTTTCCA TCATTCTCCT CAGTGCTACT 
GTGCCCAATG CCCTTGAATT TGCAGATTGG ATTGGGAGGC TGAAGCGTCG GCATCTCTAT 
GTGATCAGCA CAGCTGCCCG ACCAGTTCCC TTGGAACATT TTCTATTCAC TGGGAATAGT 
CCAAAAACCC AAGGAGAGCT TTTTCTGCTT CTGGATTCAC GAGGAGTTTT TCACACTAAG 
GGATACTATG CAGCTGTGGA GGCCAAGAAA GAAAGGACTA GCAAACATGC ACAGACCTTT 
GGGGCAAAGC AGCCAATGCA CCAAGGTGGG CCTGGACAGG ACCGAGGCAT ATACCTGTCC 
CTCTTGGCCT CCCTGCGGAG CCGTGCCCAG CTCCCTGTGG TGGTGTTCAC CTTCTCCCGG 
GGCCGCTGTG ATGAGCAGGC GTCAGGTCTC AGCTCCCTTG ACCTTACCAC CAGCTCTGAG 
AAGAGTGAGA TCCACCTTTT CCTGCAGCGA TGCCTTGCAC GACTTCGAGG CTCCGACCGC 
CAGCTACCTC AGGTACTGCA TATGTCAGAA CTCCTTCGCC GGGGTCTAGG TGTACACCAC 
AGCGGTATAT TGCCCATTCT CAAGGAGATT GTGGAAATGC TCTTCAGTCG TGGTTTGGTC 
AAGGTCCTAT TTGCTACAGA AACCTTTGCC ATGGGTGTGA ACATGCCAGC ACGGACTGTG 
GTGTTTGATT CTATGCGCAA GCATGATGGT GCAACTTTCC GTGACTTGCT CCCTGGGGAA 
TATGTACAGA TGGCAGGGCG GGCAGGGCGT CGTGGTCTGG ACCCTACAGG CACTGTCATC 
CTACTCTGTA AGGGACGTGT GCCTGAGATG GCTGATCTGC ATAGGATGAT GTTGGGAAAG 
CCTTCCCAGC TACAGTCCCA ATTTCGCCTC ACATATACAA TGATTCTTAA CTTGCTTCGG 
GTTGATGCCC TCAGAGTGGA AGATATGATG AAGAGAAGCT TTTCTGAGTT CCCTTCAAGA 
AAGGACAGCA AGGCACATGA GCAGACTTTG GCAGAATTGA CAAAGAGACT TGGGGACCTA 
GAGGAACCAG ATACCTCAGG GCAGCTTGCT GATCTTCCTG AGTATTACAG CTGGGGGGAA 
GAGCTGACAG AGACTCGGGG CCTAATCCAG CGACGGATCA TGGAATCAGT GAATGGGTTG 
AAATCTCTGT CAGTGGGAAG AGTGGTAGTG GTTAAGAATC AGGAGCATCG GAATGCACTT 
GGAGTGATCT TGCAGGTCTC CTCAGATACC ACAAACCGAG TTTTCACAAC CTTGATTCTC 
TGTGAGAAGC AGCCCCCTGA GGGGCCACTG CCTGGCAGTT CCAGATCCCT TGATGTTCCC 
TATCCTGATG ATTTAGTGGG TCACAAGCTA TTCCTCCCTG AAGGGCCTTG TGATCATACT 
GTGGCAAAAC TTCAGCCAAG TGACATCATT GCTATCACAA CAAAGATGCT ACGGGTGAAT 
GGGGAACGAA TTTTGGAAGA TTTTAGCAAG AGGCAGCAGC CAAGATTCAA GTTAGTAGCT 
TTGGGAAGGA CCCTCCCTCT CCATTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SKIV2L cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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