LOC8269491 cDNA ORF clone, Ricinus communis(castor bean)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8269491
Entrez Gene ID	8269491
Full Name	phosphoenolpyruvate carboxylase 4
Synonyms	PPC4
Gene Type	protein-coding
Organism	Ricinus communis(castor bean)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015722511.1	XP_015577997.1	phosphoenolpyruvate carboxylase 4
NM_001323722.1	NP_001310651.1	phosphoenolpyruvate carboxylase 4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Regulatory monoubiquitination of phosphoenolpyruvate carboxylase in germinating castor oil seeds.
Uhrig RG, She YM, Leach CA, Plaxton WC
The Journal of biological chemistry283(44)29650-7(2008 Oct)

Bacterial- and plant-type phosphoenolpyruvate carboxylase polypeptides interact in the hetero-oligomeric Class-2 PEPC complex of developing castor oil seeds.
Gennidakis S, Rao S, Greenham K, Uhrig RG, O'Leary B, Snedden WA, Lu C, Plaxton WC
The Plant journal : for cell and molecular biology52(5)839-49(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC8269491 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8269491 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORb40812	NM_001323722.1 Latest version!	Ricinus communis phosphoenolpyruvate carboxylase 4 (LOC8269491), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORb33267	XM_015722511.1 Latest version!	Ricinus communis phosphoenolpyruvate carboxylase 4 (LOC8269491), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8269491 ( NM_001323722.1 ) cDNA ORF clone, Ricinus communis(castor bean) -> NP_001310651.1 Ricinus communis phosphoenolpyruvate carboxylase 4 (LOC8269491), mRNA.

Price & Availability↑

CloneID	ORb40812
Clone ID Related Accession (Same CDS sequence)	NM_001323722.1
Accession Version	NM_001323722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1483372800000
Organism	Ricinus communis(castor bean)
Product	phosphoenolpyruvate carboxylase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF634318.1. ##Evidence-Data-START## Transcript exon combination :: EF634318.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1034165, SAMEA1034167 [ECO:0000350] ##Evidence-Data-END##

ATGACGGACA CCACAGATGA TATTGCAGAG GAAATCTCAT TTCAGAGTTT TGATGATGAT 
TGTAAGCTTC TTGGCAATTT GTTAAACGAT GTTTTGCAGA GAGAAGTTGG TTCTAAATTC 
ATGGAGAAGC TTGAACGCAA TCGCATCCTA GCTCAGAGTG CTTGTAATAT GAGATTGGCG 
GGGATAGAAG ATACCGCTGA ATTGCTGGAG AAGCAGCTGG CATTGGAGAT ATCAAGGATG 
ACATTAGAGG AAGCATTGAC ACTTGCTCGT GCCTTTAGTC ATTACCTTAA TTTGATGGGC 
ATTGCTGAGA CCCATCACAG GGTTCGTAAG GCACGGAGTA TGACACATCT ATCAAAATCT 
TGTGATGACA TATTTAATCA GCTGCTGCAG AGTGGCATAT CTGCAGAGGA GCTTTATGAC 
ACAGTTTGCA AGCAGGAGGT TGAAATTGTG CTCACTGCAC ATCCTACTCA AATTAATCGT 
CGTACCTTGC AATATAAGCA CATCAGAATT GCTCATCTTT TAGATTATAA TGACCGGCCT 
GATCTTACTC ATGAAGATCG AGAAATGCTG ATTGAAGATC TGGTGAGAGA AATTACTTCA 
ATATGGCAGA CAGATGAGCT TAGGCGCCAC AAGCCTACAC CAGTAGATGA AGCTAGGGCT 
GGCTTGAACA TTGTGGAGCA GTCCCTGTGG AAAGCTCTTC CCCATTATTT ACGTCGTGTC 
AGTACTGCCC TAAAGAAGCA TACAGGAAAG CCACTTCCTT TGACTTGCAC GCCAATAAGG 
TTTGGGTCTT GGATGGGGGG TGATAGAGAT GGTAATCCGA ATGTAACAGC AAAGGTCACA 
AGAGATGTTT CTCTTTTATC TAGGTGGATG GCTGTTGACC TTTACATTCG AGAAGTTGAT 
AGCCTGAGAT TTGAACTATC CATGGTTCAA TGCAGTGATA GATTGTTGAA AGTGGCAAAT 
GACATTTTAA TAGAAGAAAC TTCATCTGAA GATCACCATG AGAGTTGGAA TCAACCTGCA 
AGCAGAAGTC AAACAAAGTT TCCTAGAAAA TCTCTTCCTA CACAACTTCC ACCTAGAGCT 
GATCTTCCTG CTTGCACTGA ATGTAATGAT GGTGAATCTC AGTATCCCAA ACTAGAACTT 
CCAGGAACTG ATTACATGCC CTTTAATCGC CAGGAAGCTC TAGGTTCTTC ATATTCAGAA 
TCTTCATCCC AGGATATCAA TCATGGTTTA CCTAAAACAA CTGGAAATGG AAGTGTTGCT 
AATTCTAGTG GATCTCCGCG GGCATCTTTC AGCTCTGCTC AACTGGTTGC ACAGAGGAAA 
CTATTTGCAG AATCCAAGAT AGGAAGATCT AGCTTCCAGA AGCTTCTAGA ACCAAGTCTG 
CCTCAACGTC CTGGAATTGC TCCTTATAGA ATTGTTCTTG GAAATGTAAA AGATAAGCTT 
ATGAGGACAA GAAGACGTCT GGAACTTCTT CTAGAGGATC TTCCTTGTGA ATATGATCAA 
TGGGATTACT ATGAGACAAC AGATCAATTA TTGGATCCAC TGCTTCTGTG CTACGAGTCT 
CTTCAATCAT GTGGAGCTGG GGTTCTAGCT GATGGTCGGC TAGCTGACCT GATAAGAAGA 
GTTGCTACAT TTGGGATGGT GTTAATGAAG CTAGACTTGC GCCAGGAATC TGGTAGACAT 
GCTGATACTC TTGATGCAAT CACCAAATAT TTGGAAATGG GCACGTATAG TGAGTGGGAT 
GAAGAAAAGA AGCTGGAATT TCTGACAAGA GAACTGAAAG GAAAACGGCC ACTGGTTCCT 
CCTACTATTG AGGTTGCTCC TGATGTTAAA GAAGTCTTGG ATGCTTTCCG TGTTGCTGCT 
GAGCTGGGGA GTGATTCACT TGGAGCTTAT GTGATTTCTA TGGCATCCAA TGCAAGTGAT 
GTCCTTGCTG TGGAGCTTCT GCAGAAGGAT GCCCGGCTTG CTGTTAGTGG GGAGCTAGGA 
AGACCATGCC CTGGTGGAAC GTTGCGAGTA GTTCCGTTGT TTGAAACTGT GAAAGACCTG 
AGAGGTGCTG GTTCGGTGAT CAGAAAACTG TTATCAATTG ACTGGTATAG AGAACACATA 
ATTAAGAACC ATAACGGGCA TCAGGAGGTG ATGGTTGGCT ATTCTGATTC TGGTAAAGAT 
GCTGGACGCT TTACTGCTGC ATGGGAACTT TACAAAGCCC AAGAGGATGT TGTGGCAGCC 
TGTAATGATT TTGGTATCAA AGTAACACTA TTCCATGGGC GTGGAGGGAG TATTGGTCGT 
GGTGGTGGCC CCACATATCT TGCCATTCAA TCCCAACCAC CTGGTTCAGT TATGGGTACT 
CTTCGGTCAA CTGAGCAAGG AGAAATGGTG CAGGCCAAGT TTGGGCTGCC ACATACTGCC 
ATCAGACAAC TAGAGATATA CACAACTGCT GTGCTGCTTG CAACCCTTCG TCCTCCACAT 
CCACCTCGAG AAGAACAATG GCGCAATGTC ATGGAGGAGA TTTCAAAAAT CAGTTGCCAG 
AATTACCGGA GCACAGTCTA TGAAAACCCA GAATTCCTCG CCTACTTCCA TGAGGCTACT 
CCCCAGGCTG AGCTTGGCTT TCTTAACATA GGAAGCCGCC CCACAAGAAG AAAGAGCTCA 
ACTGGTATTG GACATCTTCG TGCCATTCCA TGGGTATTCG CATGGACCCA GACCAGATTT 
GTTCTTCCTG CTTGGCTTGG AGTTGGAGCA GGTTTAAAGG GTGCTTGTGA GAAGGGATTT 
ACTGAAGACT TGAAAGCAAT GTACAAAGAA TGGCCTTTCT TCCAATCTAC TATTGATCTT 
ATAGAGATGG TACTAGGGAA GGCAGACATT CCTATAGCCA AGCACTATGA TGAAGTTCTT 
GTATCTGAGA GCAGGCGAGA GCTTGGAGCT GAGCTTAGGA GTGAGCTCTT AACAACAGAA 
AAGTATGTGT TGGTGGTTAG TGGGCATGAG AAACTATCTC AGAACAACCG CAGCCTGCGG 
AGGCTAATAG AGAGCAGGCT GCCTTATCTC AATCCTATGA ATATGTTGCA GGTGGAGGTT 
CTAAAGAGGT TGAGGCGGGA CGACGACAAC AATAAGCTCA GAGATGCCCT GCTTATTACC 
ATAAATGGGA TTGCTGCTGG AATGAGGAAC ACAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310651.1
CDS	1..3159
Translation	MTDTTDDIAEEISFQSFDDDCKLLGNLLNDVLQREVGSKFMEKLERNRILAQSACNMRLAGIEDTAELLEKQLALEISRMTLEEALTLARAFSHYLNLMGIAETHHRVRKARSMTHLSKSCDDIFNQLLQSGISAEELYDTVCKQEVEIVLTAHPTQINRRTLQYKHIRIAHLLDYNDRPDLTHEDREMLIEDLVREITSIWQTDELRRHKPTPVDEARAGLNIVEQSLWKALPHYLRRVSTALKKHTGKPLPLTCTPIRFGSWMGGDRDGNPNVTAKVTRDVSLLSRWMAVDLYIREVDSLRFELSMVQCSDRLLKVANDILIEETSSEDHHESWNQPASRSQTKFPRKSLPTQLPPRADLPACTECNDGESQYPKLELPGTDYMPFNRQEALGSSYSESSSQDINHGLPKTTGNGSVANSSGSPRASFSSAQLVAQRKLFAESKIGRSSFQKLLEPSLPQRPGIAPYRIVLGNVKDKLMRTRRRLELLLEDLPCEYDQWDYYETTDQLLDPLLLCYESLQSCGAGVLADGRLADLIRRVATFGMVLMKLDLRQESGRHADTLDAITKYLEMGTYSEWDEEKKLEFLTRELKGKRPLVPPTIEVAPDVKEVLDAFRVAAELGSDSLGAYVISMASNASDVLAVELLQKDARLAVSGELGRPCPGGTLRVVPLFETVKDLRGAGSVIRKLLSIDWYREHIIKNHNGHQEVMVGYSDSGKDAGRFTAAWELYKAQEDVVAACNDFGIKVTLFHGRGGSIGRGGGPTYLAIQSQPPGSVMGTLRSTEQGEMVQAKFGLPHTAIRQLEIYTTAVLLATLRPPHPPREEQWRNVMEEISKISCQNYRSTVYENPEFLAYFHEATPQAELGFLNIGSRPTRRKSSTGIGHLRAIPWVFAWTQTRFVLPAWLGVGAGLKGACEKGFTEDLKAMYKEWPFFQSTIDLIEMVLGKADIPIAKHYDEVLVSESRRELGAELRSELLTTEKYVLVVSGHEKLSQNNRSLRRLIESRLPYLNPMNMLQVEVLKRLRRDDDNNKLRDALLITINGIAAGMRNTG

Target ORF information:

RefSeq Version	NM_001323722.1
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis phosphoenolpyruvate carboxylase 4 (LOC8269491), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323722.1

ORF Insert Sequence:

ATGACGGACA CCACAGATGA TATTGCAGAG GAAATCTCAT TTCAGAGTTT TGATGATGAT 
TGTAAGCTTC TTGGCAATTT GTTAAACGAT GTTTTGCAGA GAGAAGTTGG TTCTAAATTC 
ATGGAGAAGC TTGAACGCAA TCGCATCCTA GCTCAGAGTG CTTGTAATAT GAGATTGGCG 
GGGATAGAAG ATACCGCTGA ATTGCTGGAG AAGCAGCTGG CATTGGAGAT ATCAAGGATG 
ACATTAGAGG AAGCATTGAC ACTTGCTCGT GCCTTTAGTC ATTACCTTAA TTTGATGGGC 
ATTGCTGAGA CCCATCACAG GGTTCGTAAG GCACGGAGTA TGACACATCT ATCAAAATCT 
TGTGATGACA TATTTAATCA GCTGCTGCAG AGTGGCATAT CTGCAGAGGA GCTTTATGAC 
ACAGTTTGCA AGCAGGAGGT TGAAATTGTG CTCACTGCAC ATCCTACTCA AATTAATCGT 
CGTACCTTGC AATATAAGCA CATCAGAATT GCTCATCTTT TAGATTATAA TGACCGGCCT 
GATCTTACTC ATGAAGATCG AGAAATGCTG ATTGAAGATC TGGTGAGAGA AATTACTTCA 
ATATGGCAGA CAGATGAGCT TAGGCGCCAC AAGCCTACAC CAGTAGATGA AGCTAGGGCT 
GGCTTGAACA TTGTGGAGCA GTCCCTGTGG AAAGCTCTTC CCCATTATTT ACGTCGTGTC 
AGTACTGCCC TAAAGAAGCA TACAGGAAAG CCACTTCCTT TGACTTGCAC GCCAATAAGG 
TTTGGGTCTT GGATGGGGGG TGATAGAGAT GGTAATCCGA ATGTAACAGC AAAGGTCACA 
AGAGATGTTT CTCTTTTATC TAGGTGGATG GCTGTTGACC TTTACATTCG AGAAGTTGAT 
AGCCTGAGAT TTGAACTATC CATGGTTCAA TGCAGTGATA GATTGTTGAA AGTGGCAAAT 
GACATTTTAA TAGAAGAAAC TTCATCTGAA GATCACCATG AGAGTTGGAA TCAACCTGCA 
AGCAGAAGTC AAACAAAGTT TCCTAGAAAA TCTCTTCCTA CACAACTTCC ACCTAGAGCT 
GATCTTCCTG CTTGCACTGA ATGTAATGAT GGTGAATCTC AGTATCCCAA ACTAGAACTT 
CCAGGAACTG ATTACATGCC CTTTAATCGC CAGGAAGCTC TAGGTTCTTC ATATTCAGAA 
TCTTCATCCC AGGATATCAA TCATGGTTTA CCTAAAACAA CTGGAAATGG AAGTGTTGCT 
AATTCTAGTG GATCTCCGCG GGCATCTTTC AGCTCTGCTC AACTGGTTGC ACAGAGGAAA 
CTATTTGCAG AATCCAAGAT AGGAAGATCT AGCTTCCAGA AGCTTCTAGA ACCAAGTCTG 
CCTCAACGTC CTGGAATTGC TCCTTATAGA ATTGTTCTTG GAAATGTAAA AGATAAGCTT 
ATGAGGACAA GAAGACGTCT GGAACTTCTT CTAGAGGATC TTCCTTGTGA ATATGATCAA 
TGGGATTACT ATGAGACAAC AGATCAATTA TTGGATCCAC TGCTTCTGTG CTACGAGTCT 
CTTCAATCAT GTGGAGCTGG GGTTCTAGCT GATGGTCGGC TAGCTGACCT GATAAGAAGA 
GTTGCTACAT TTGGGATGGT GTTAATGAAG CTAGACTTGC GCCAGGAATC TGGTAGACAT 
GCTGATACTC TTGATGCAAT CACCAAATAT TTGGAAATGG GCACGTATAG TGAGTGGGAT 
GAAGAAAAGA AGCTGGAATT TCTGACAAGA GAACTGAAAG GAAAACGGCC ACTGGTTCCT 
CCTACTATTG AGGTTGCTCC TGATGTTAAA GAAGTCTTGG ATGCTTTCCG TGTTGCTGCT 
GAGCTGGGGA GTGATTCACT TGGAGCTTAT GTGATTTCTA TGGCATCCAA TGCAAGTGAT 
GTCCTTGCTG TGGAGCTTCT GCAGAAGGAT GCCCGGCTTG CTGTTAGTGG GGAGCTAGGA 
AGACCATGCC CTGGTGGAAC GTTGCGAGTA GTTCCGTTGT TTGAAACTGT GAAAGACCTG 
AGAGGTGCTG GTTCGGTGAT CAGAAAACTG TTATCAATTG ACTGGTATAG AGAACACATA 
ATTAAGAACC ATAACGGGCA TCAGGAGGTG ATGGTTGGCT ATTCTGATTC TGGTAAAGAT 
GCTGGACGCT TTACTGCTGC ATGGGAACTT TACAAAGCCC AAGAGGATGT TGTGGCAGCC 
TGTAATGATT TTGGTATCAA AGTAACACTA TTCCATGGGC GTGGAGGGAG TATTGGTCGT 
GGTGGTGGCC CCACATATCT TGCCATTCAA TCCCAACCAC CTGGTTCAGT TATGGGTACT 
CTTCGGTCAA CTGAGCAAGG AGAAATGGTG CAGGCCAAGT TTGGGCTGCC ACATACTGCC 
ATCAGACAAC TAGAGATATA CACAACTGCT GTGCTGCTTG CAACCCTTCG TCCTCCACAT 
CCACCTCGAG AAGAACAATG GCGCAATGTC ATGGAGGAGA TTTCAAAAAT CAGTTGCCAG 
AATTACCGGA GCACAGTCTA TGAAAACCCA GAATTCCTCG CCTACTTCCA TGAGGCTACT 
CCCCAGGCTG AGCTTGGCTT TCTTAACATA GGAAGCCGCC CCACAAGAAG AAAGAGCTCA 
ACTGGTATTG GACATCTTCG TGCCATTCCA TGGGTATTCG CATGGACCCA GACCAGATTT 
GTTCTTCCTG CTTGGCTTGG AGTTGGAGCA GGTTTAAAGG GTGCTTGTGA GAAGGGATTT 
ACTGAAGACT TGAAAGCAAT GTACAAAGAA TGGCCTTTCT TCCAATCTAC TATTGATCTT 
ATAGAGATGG TACTAGGGAA GGCAGACATT CCTATAGCCA AGCACTATGA TGAAGTTCTT 
GTATCTGAGA GCAGGCGAGA GCTTGGAGCT GAGCTTAGGA GTGAGCTCTT AACAACAGAA 
AAGTATGTGT TGGTGGTTAG TGGGCATGAG AAACTATCTC AGAACAACCG CAGCCTGCGG 
AGGCTAATAG AGAGCAGGCT GCCTTATCTC AATCCTATGA ATATGTTGCA GGTGGAGGTT 
CTAAAGAGGT TGAGGCGGGA CGACGACAAC AATAAGCTCA GAGATGCCCT GCTTATTACC 
ATAAATGGGA TTGCTGCTGG AATGAGGAAC ACAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8269491 ( XM_015722511.1 ) cDNA ORF clone, Ricinus communis(castor bean) -> XP_015577997.1 Ricinus communis phosphoenolpyruvate carboxylase 4 (LOC8269491), mRNA.

Price & Availability↑

CloneID	ORb33267
Clone ID Related Accession (Same CDS sequence)	XM_015722511.1
Accession Version	XM_015722511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1456243200000
Organism	Ricinus communis(castor bean)
Product	phosphoenolpyruvate carboxylase 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002994440.1) and transcript sequence (EF634318.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ricinus communis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 279 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGACGGACA CAACAGATGA TATAGCAGAG GAAATCTCAT TTCAGAGTTT TGATGATGAT 
TGTAAGCTTC TTGGCAATTT GTTAAACGAT GTTTTGCAGA GAGAAGTTGG TTCTAAATTC 
ATGGAGAAGC TTGAACGCAA TCGCATCCTA GCTCAGAGTG CTTGTAATAT GAGATTGGCG 
GGGATAGAAG ATACCGCTGA ATTGCTGGAG AAGCAGCTGG CATTGGAGAT ATCAAGGATG 
ACATTAGAGG AAGCATTGAC ACTTGCTCGT GCCTTTAGTC ATTACCTTAA TTTGATGGGC 
ATTGCTGAGA CCCATCACAG GGTTCGTAAG GCACGGAGTA TGACACATCT ATCAAAATCT 
TGTGATGACA TATTTAATCA GCTGCTGCAG AGTGGCATAT CTGCAGAGGA GCTTTATGAC 
ACAGTTTGCA AGCAGGAGGT TGAAATTGTG CTCACTGCAC ATCCTACTCA AATTAATCGT 
CGTACCTTGC AATATAAGCA CATCAGAATT GCTCATCTTT TAGATTATAA TGACCGGCCT 
GATCTTACTC ATGAAGATCG AGAAATGCTG ATTGAAGATC TGGTGAGAGA AATTACTTCA 
ATATGGCAGA CAGATGAGCT TAGGCGCCAC AAGCCTACAC CAGTAGATGA AGCTAGGGCT 
GGCTTGAACA TTGTGGAGCA GTCCCTGTGG AAAGCTCTTC CCCATTATTT ACGTCGTGTC 
AGTACTGCCC TAAAGAAGCA TACAGGAAAG CCACTTCCTT TGACTTGCAT GCCAATAAGG 
TTTGGGTCTT GGATGGGGGG TGATAGAGAT GGTAATCCGA ATGTAACAGC AAAGGTCACA 
AGAGATGTTT CTCTTTTATC TAGGTGGATG GCTGTTGACC TTTACATTCG AGAAGTTGAT 
AGCCTGAGAT TTGAACTATC CATGGTTCAA TGCAGTGATA GATTGTTGAA AGTGGCAAAT 
GACATTTTAA TAGAAGAAAC TTCATCTGAA GATCACCATG AGAGTTGGAA TCAACCTGCA 
AGCAGAAGTC AAACAAAGTT TCCTAGAAAA TCTCTTCCTA CACAACTTCC ACCTAGAGCT 
GATCTTCCTG CTTGCACTGA ATGTAATGAT GGTGAATCTC AGTATCCCAA ACTAGAACTT 
CCAGGAACTG ATTACATGCC CTTTAATCGC CAGGAAGCTC TAGGTTCTTC ATATTCAGAA 
TCTTCATCCC AGGATATCAA TCATGGTTTA CCTAAAACAA CTGGAAATGG AAGTGTTGCT 
AATTCTAGTG GATCTCCGCG GGCATCTTTC AGCTCTGCTC AACTGGTTGC ACAGAGGAAA 
CTATTTGCAG AATCCAAGAT AGGAAGATCT AGCTTCCAGA AGCTTCTAGA ACCAAGTCTG 
CCTCAACGTC CTGGAATTGC TCCTTATAGA ATTGTTCTTG GAAATGTAAA AGATAAGCTT 
ATGAGGACAA GAAGACGTCT GGAACTTCTT CTAGAGGATC TTCCTTGTGA ATATGATCAA 
TGGGATTACT ATGAGACAAC AGATCAATTA TTGGATCCAC TGCTTCTGTG CTACGAGTCT 
CTTCAATCAT GTGGAGCTGG GGTTCTAGCT GATGGTCGGC TAGCTGACCT GATAAGAAGA 
GTTGCTACAT TTGGGATGGT GTTAATGAAG CTAGACTTGC GCCAGGAATC TGGTAGACAT 
GCTGATACTC TTGATGCAAT CACCAAATAT TTGGAAATGG GCACGTATAG TGAGTGGGAT 
GAAGAAAAGA AGCTGGAATT TCTGACAAGA GAACTGAAAG GAAAACGGCC ACTGGTTCCT 
CCTACTATTG AGGTTGCTCC TGATGTTAAA GAAGTCTTGG ATGCTTTCCG TGTTGCTGCT 
GAGCTGGGGA GTGATTCACT TGGAGCTTAT GTGATTTCTA TGGCATCCAA TGCAAGTGAT 
GTCCTTGCTG TGGAGCTTCT GCAGAAGGAT GCCCGGCTTG CTGTTAGTGG GGAGCTAGGA 
AGACCATGCC CTGGTGGAAC GTTGCGAGTA GTTCCGTTGT TTGAAACTGT GAAAGACCTG 
AGAGGTGCTG GTTCGGTGAT CAGAAAACTG TTATCAATTG ACTGGTATAG AGAACACATA 
ATTAAGAACC ATAACGGGCA TCAGGAGGTG ATGGTTGGCT ATTCTGATTC TGGTAAAGAT 
GCTGGACGCT TTACTGCTGC ATGGGAACTT TACAAAGCCC AAGAGGATGT TGTGGCAGCC 
TGTAATGATT TTGGTATCAA AGTAACACTA TTCCATGGGC GTGGAGGGAG TATTGGTCGT 
GGTGGTGGCC CCACATATCT TGCCATTCAA TCCCAACCAC CTGGTTCAGT TATGGGTACT 
CTTCGGTCAA CTGAGCAAGG AGAAATGGTG CAGGCCAAGT TTGGGCTGCC ACATACTGCC 
ATCAGACAAC TAGAGATATA CACAACTGCT GTGCTGCTTG CAACCCTTCG TCCTCCACAT 
CCACCTCGAG AAGAAAAATG GCGCAATGTC ATGGAGGAGA TTTCAAAAAT CAGTTGCCAG 
AATTACCGGA GCACAGTCTA TGAAAACCCA GAATTCCTCG CCTACTTCCA TGAGGCTACT 
CCCCAGGCTG AGCTTGGCTT TCTTAACATA GGAAGCCGCC CCACAAGAAG AAAGAGCTCA 
ACTGGTATTG GACATCTTCG TGCCATTCCA TGGGTATTCG CATGGACCCA GACCAGATTT 
GTTCTTCCTG CTTGGCTTGG AGTTGGAGCA GGTTTAAAGG GTGCTTGTGA GAAGGGATTT 
ACTGAAGACT TGAAAGCAAT GTACAAAGAA TGGCCTTTCT TCCAATCTAC TATTGATCTT 
ATAGAGATGG TACTAGGGAA GGCAGACATT CCTATAGCCA AGCACTATGA TGAAGTTCTT 
GTATCTGAGA GCAGGCGAGA GCTTGGAGCT GAGCTTAGGA GTGAGCTCTT AACAACAGAA 
AAGTATGTGT TGGTGGTTAG TGGGCATGAG AAACTATCTC AGAACAACCG CAGCCTGCGG 
AGGCTAATAG AGAGCAGGCT GCCTTATCTC AATCCTATGA ATATGTTGCA GGTGGAGGTT 
CTAAAGAGGT TGAGGCGGGA CGACGACAAC AATAAGCTCA GAGATGCCCT GCTTATTACC 
ATAAATGGGA TTGCTGCTGG AATGAGGAAC ACAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015577997.1
CDS	1..3159
Translation	MTDTTDDIAEEISFQSFDDDCKLLGNLLNDVLQREVGSKFMEKLERNRILAQSACNMRLAGIEDTAELLEKQLALEISRMTLEEALTLARAFSHYLNLMGIAETHHRVRKARSMTHLSKSCDDIFNQLLQSGISAEELYDTVCKQEVEIVLTAHPTQINRRTLQYKHIRIAHLLDYNDRPDLTHEDREMLIEDLVREITSIWQTDELRRHKPTPVDEARAGLNIVEQSLWKALPHYLRRVSTALKKHTGKPLPLTCMPIRFGSWMGGDRDGNPNVTAKVTRDVSLLSRWMAVDLYIREVDSLRFELSMVQCSDRLLKVANDILIEETSSEDHHESWNQPASRSQTKFPRKSLPTQLPPRADLPACTECNDGESQYPKLELPGTDYMPFNRQEALGSSYSESSSQDINHGLPKTTGNGSVANSSGSPRASFSSAQLVAQRKLFAESKIGRSSFQKLLEPSLPQRPGIAPYRIVLGNVKDKLMRTRRRLELLLEDLPCEYDQWDYYETTDQLLDPLLLCYESLQSCGAGVLADGRLADLIRRVATFGMVLMKLDLRQESGRHADTLDAITKYLEMGTYSEWDEEKKLEFLTRELKGKRPLVPPTIEVAPDVKEVLDAFRVAAELGSDSLGAYVISMASNASDVLAVELLQKDARLAVSGELGRPCPGGTLRVVPLFETVKDLRGAGSVIRKLLSIDWYREHIIKNHNGHQEVMVGYSDSGKDAGRFTAAWELYKAQEDVVAACNDFGIKVTLFHGRGGSIGRGGGPTYLAIQSQPPGSVMGTLRSTEQGEMVQAKFGLPHTAIRQLEIYTTAVLLATLRPPHPPREEKWRNVMEEISKISCQNYRSTVYENPEFLAYFHEATPQAELGFLNIGSRPTRRKSSTGIGHLRAIPWVFAWTQTRFVLPAWLGVGAGLKGACEKGFTEDLKAMYKEWPFFQSTIDLIEMVLGKADIPIAKHYDEVLVSESRRELGAELRSELLTTEKYVLVVSGHEKLSQNNRSLRRLIESRLPYLNPMNMLQVEVLKRLRRDDDNNKLRDALLITINGIAAGMRNTG

Target ORF information:

RefSeq Version	XM_015722511.1
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis phosphoenolpyruvate carboxylase 4 (LOC8269491), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015722511.1

ORF Insert Sequence:

ATGACGGACA CAACAGATGA TATAGCAGAG GAAATCTCAT TTCAGAGTTT TGATGATGAT 
TGTAAGCTTC TTGGCAATTT GTTAAACGAT GTTTTGCAGA GAGAAGTTGG TTCTAAATTC 
ATGGAGAAGC TTGAACGCAA TCGCATCCTA GCTCAGAGTG CTTGTAATAT GAGATTGGCG 
GGGATAGAAG ATACCGCTGA ATTGCTGGAG AAGCAGCTGG CATTGGAGAT ATCAAGGATG 
ACATTAGAGG AAGCATTGAC ACTTGCTCGT GCCTTTAGTC ATTACCTTAA TTTGATGGGC 
ATTGCTGAGA CCCATCACAG GGTTCGTAAG GCACGGAGTA TGACACATCT ATCAAAATCT 
TGTGATGACA TATTTAATCA GCTGCTGCAG AGTGGCATAT CTGCAGAGGA GCTTTATGAC 
ACAGTTTGCA AGCAGGAGGT TGAAATTGTG CTCACTGCAC ATCCTACTCA AATTAATCGT 
CGTACCTTGC AATATAAGCA CATCAGAATT GCTCATCTTT TAGATTATAA TGACCGGCCT 
GATCTTACTC ATGAAGATCG AGAAATGCTG ATTGAAGATC TGGTGAGAGA AATTACTTCA 
ATATGGCAGA CAGATGAGCT TAGGCGCCAC AAGCCTACAC CAGTAGATGA AGCTAGGGCT 
GGCTTGAACA TTGTGGAGCA GTCCCTGTGG AAAGCTCTTC CCCATTATTT ACGTCGTGTC 
AGTACTGCCC TAAAGAAGCA TACAGGAAAG CCACTTCCTT TGACTTGCAT GCCAATAAGG 
TTTGGGTCTT GGATGGGGGG TGATAGAGAT GGTAATCCGA ATGTAACAGC AAAGGTCACA 
AGAGATGTTT CTCTTTTATC TAGGTGGATG GCTGTTGACC TTTACATTCG AGAAGTTGAT 
AGCCTGAGAT TTGAACTATC CATGGTTCAA TGCAGTGATA GATTGTTGAA AGTGGCAAAT 
GACATTTTAA TAGAAGAAAC TTCATCTGAA GATCACCATG AGAGTTGGAA TCAACCTGCA 
AGCAGAAGTC AAACAAAGTT TCCTAGAAAA TCTCTTCCTA CACAACTTCC ACCTAGAGCT 
GATCTTCCTG CTTGCACTGA ATGTAATGAT GGTGAATCTC AGTATCCCAA ACTAGAACTT 
CCAGGAACTG ATTACATGCC CTTTAATCGC CAGGAAGCTC TAGGTTCTTC ATATTCAGAA 
TCTTCATCCC AGGATATCAA TCATGGTTTA CCTAAAACAA CTGGAAATGG AAGTGTTGCT 
AATTCTAGTG GATCTCCGCG GGCATCTTTC AGCTCTGCTC AACTGGTTGC ACAGAGGAAA 
CTATTTGCAG AATCCAAGAT AGGAAGATCT AGCTTCCAGA AGCTTCTAGA ACCAAGTCTG 
CCTCAACGTC CTGGAATTGC TCCTTATAGA ATTGTTCTTG GAAATGTAAA AGATAAGCTT 
ATGAGGACAA GAAGACGTCT GGAACTTCTT CTAGAGGATC TTCCTTGTGA ATATGATCAA 
TGGGATTACT ATGAGACAAC AGATCAATTA TTGGATCCAC TGCTTCTGTG CTACGAGTCT 
CTTCAATCAT GTGGAGCTGG GGTTCTAGCT GATGGTCGGC TAGCTGACCT GATAAGAAGA 
GTTGCTACAT TTGGGATGGT GTTAATGAAG CTAGACTTGC GCCAGGAATC TGGTAGACAT 
GCTGATACTC TTGATGCAAT CACCAAATAT TTGGAAATGG GCACGTATAG TGAGTGGGAT 
GAAGAAAAGA AGCTGGAATT TCTGACAAGA GAACTGAAAG GAAAACGGCC ACTGGTTCCT 
CCTACTATTG AGGTTGCTCC TGATGTTAAA GAAGTCTTGG ATGCTTTCCG TGTTGCTGCT 
GAGCTGGGGA GTGATTCACT TGGAGCTTAT GTGATTTCTA TGGCATCCAA TGCAAGTGAT 
GTCCTTGCTG TGGAGCTTCT GCAGAAGGAT GCCCGGCTTG CTGTTAGTGG GGAGCTAGGA 
AGACCATGCC CTGGTGGAAC GTTGCGAGTA GTTCCGTTGT TTGAAACTGT GAAAGACCTG 
AGAGGTGCTG GTTCGGTGAT CAGAAAACTG TTATCAATTG ACTGGTATAG AGAACACATA 
ATTAAGAACC ATAACGGGCA TCAGGAGGTG ATGGTTGGCT ATTCTGATTC TGGTAAAGAT 
GCTGGACGCT TTACTGCTGC ATGGGAACTT TACAAAGCCC AAGAGGATGT TGTGGCAGCC 
TGTAATGATT TTGGTATCAA AGTAACACTA TTCCATGGGC GTGGAGGGAG TATTGGTCGT 
GGTGGTGGCC CCACATATCT TGCCATTCAA TCCCAACCAC CTGGTTCAGT TATGGGTACT 
CTTCGGTCAA CTGAGCAAGG AGAAATGGTG CAGGCCAAGT TTGGGCTGCC ACATACTGCC 
ATCAGACAAC TAGAGATATA CACAACTGCT GTGCTGCTTG CAACCCTTCG TCCTCCACAT 
CCACCTCGAG AAGAAAAATG GCGCAATGTC ATGGAGGAGA TTTCAAAAAT CAGTTGCCAG 
AATTACCGGA GCACAGTCTA TGAAAACCCA GAATTCCTCG CCTACTTCCA TGAGGCTACT 
CCCCAGGCTG AGCTTGGCTT TCTTAACATA GGAAGCCGCC CCACAAGAAG AAAGAGCTCA 
ACTGGTATTG GACATCTTCG TGCCATTCCA TGGGTATTCG CATGGACCCA GACCAGATTT 
GTTCTTCCTG CTTGGCTTGG AGTTGGAGCA GGTTTAAAGG GTGCTTGTGA GAAGGGATTT 
ACTGAAGACT TGAAAGCAAT GTACAAAGAA TGGCCTTTCT TCCAATCTAC TATTGATCTT 
ATAGAGATGG TACTAGGGAA GGCAGACATT CCTATAGCCA AGCACTATGA TGAAGTTCTT 
GTATCTGAGA GCAGGCGAGA GCTTGGAGCT GAGCTTAGGA GTGAGCTCTT AACAACAGAA 
AAGTATGTGT TGGTGGTTAG TGGGCATGAG AAACTATCTC AGAACAACCG CAGCCTGCGG 
AGGCTAATAG AGAGCAGGCT GCCTTATCTC AATCCTATGA ATATGTTGCA GGTGGAGGTT 
CTAAAGAGGT TGAGGCGGGA CGACGACAAC AATAAGCTCA GAGATGCCCT GCTTATTACC 
ATAAATGGGA TTGCTGCTGG AATGAGGAAC ACAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulatory monoubiquitination of phosphoenolpyruvate carboxylase in germinating castor oil seeds.
The Journal of biological chemistry283(44)29650-7(2008 Oct)
Uhrig RG,She YM,Leach CA,Plaxton WC

Bacterial- and plant-type phosphoenolpyruvate carboxylase polypeptides interact in the hetero-oligomeric Class-2 PEPC complex of developing castor oil seeds.
The Plant journal : for cell and molecular biology52(5)839-49(2007 Dec)
Gennidakis S,Rao S,Greenham K,Uhrig RG,O'Leary B,Snedden WA,Lu C,Plaxton WC

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LOC8269491 cDNA ORF clone, Ricinus communis(castor bean)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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