LOC8259784 cDNA ORF clone, Ricinus communis(castor bean)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8259784
Entrez Gene ID	8259784
Full Name	putative pentatricopeptide repeat-containing protein At5g06400, mitochondrial
Gene Type	protein-coding
Organism	Ricinus communis(castor bean)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002524043.3	XP_002524089.1	putative pentatricopeptide repeat-containing protein At5g06400, mitochondrial
XM_002524043.2	XP_002524089.1	putative pentatricopeptide repeat-containing protein At5g06400, mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC8259784 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8259784 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORb01656	XM_002524043.3 Latest version!	Ricinus communis putative pentatricopeptide repeat-containing protein At5g06400, mitochondrial (LOC8259784), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
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ORb01656	XM_002524043.2	Ricinus communis putative pentatricopeptide repeat-containing protein At5g06400, mitochondrial (LOC8259784), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8259784 ( XM_002524043.3 ) cDNA ORF clone, Ricinus communis(castor bean) -> XP_002524089.1 Ricinus communis putative pentatricopeptide repeat-containing protein At5g06400, mitochondrial (LOC8259784), mRNA.

Price & Availability↑

CloneID	ORb01656
Clone ID Related Accession (Same CDS sequence)	XM_002524043.3 , XM_002524043.2
Accession Version	XM_002524043.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526918400000
Organism	Ricinus communis(castor bean)
Product	putative pentatricopeptide repeat-containing protein At5g06400, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002994432.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 23, 2018 this sequence version replaced XM_002524043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ricinus communis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCATA TAATCAAACT CCACTTACTG GGTTCAAACT CAAGCTATAA GCTTTACAGT 
TCTCAGCTCT CCATACATCA GAGTTTTTGC CTTTCTAGCT TCTCTAAATC GTCTAAACTT 
TCACATAAGC AATCCCAAAA AACCGAATTG CAAGGCCAAT CAGAAATAAG TACTACTGTT 
ACTATCAATT CACTCTTTAA GGAAATAACT GAGATTTTAG GAGCTGATAA TTTTATCCCA 
GATAAACACC CATCTGGTTT TCTAATATCA AAAGAAAATC ACCAGAAGCA TGATGAGTTA 
AATGCGGAAT TAAAGACTGG CACACGAGGT ATTTATAAAA ATGTCGAGCC GAACAATGTG 
TTGCGAGAGG AGGAGGTTTT TCTGGGTTTG GATGATACCC AGATAAGGGA TTTGAATGAA 
ATTGATGTTA GCCCTGTCGT TCGTGAGATT ACTGGGATTG TTCGCGCAGA AAATGACCTG 
GTTGCGATGG AGGAGAGGTT AGAGAATCTG GGGTTTTATT TTGAACCTGA GATCGTTGAA 
AAAGTATTGA AAAGATGCTT TAAAGTGCCA CATTTAGCTT TTAGGTTTTT CAGTTGGGTG 
AAGATGAGAG ATGGGTTTTG TCATACAACC AAGACTTATT ATACCATGCT GTATATAGCT 
GGGGAAGCTA AAGAATTTAA GGTAGTGGAT GATTTGGTAG AGGAAATGGA AAAGAACTCT 
TGCGAAAAGG ATATCAAGAC ATGGAGTATC CTTATCTCAC AATTTGGAAA GGCAAAGTTG 
ATTGGCAAAG CCTTGTTGTT TTTTGAGAAG ATGAAGACAT CTGGGTGTGA ACCCGATGAA 
AAAATTTATA AAATGATGGT GCATTCTCTT TGTAATGCTG GGAAAGGTGA GGTTGCATTG 
GCATTTTACA AGGAGATGGT ACAAAAGGAC ATGAGACTGG ATTTAAGTTT GTACAAAATA 
TTACTCATTA GCATGGCAAA GTCGGGAGAT GTTGGCGCTG TTCACTTGGT TGCAAATGAC 
ATGAGTAGGC TTTCACAGAT TCCGGAACAT GATGTTAATG TTTGCATACT GAAGAGTTTT 
TGTGTGGCTG GGAAAATTAG AGAAGCTCTA GAATTGATTC GTGACCTCAA GAACAAAGAT 
ATACTAATTG ACTATGAATA TCTTGGGACT TTAGTGAAAG GTCTGTGCAG GGGTGATAGG 
ATTACTGATG CTGTGGAAAT TGTTGAAATT ATGAAGAAAA GAAATCTTAT TGATGCAAAG 
ATTTATGGGA TTATCATAAA TGGATACTTG AGAAAAAAAG ACCTTTCAAA GGCAATTGAG 
CTATTTCAGA GAATGAAAGA ATCTGGTATT CAACCAATTA CTTCCACCTA TACAGAGTTA 
ATGCAATGCC TGTTCAATTT GAACCAGTAT GACAAAGGCT TTGAGCTATT TAACGAGATG 
CTGGAAAGAG GAATTAGGGT TGATAGTGTG GCAACTATGG CAATTGTTGC CGCTCATGTT 
CGTCAAAACC ATATATCAGA AGCATGGGAA GTGTTCAATA CCATGAAGGA TAAGGGAGCC 
AATCCTACTT GGAAATCTTA TTCCATATTC ATTAAGGAGC TTTGCAGAGT TTCAAGAACA 
GATGAGATTT TGAAAGTATT GTACAAAATG CAGGCCTCCA AGATATTCAT TAATAATGAT 
ATATTCAACT TAGCGATAGC TTTTATGGAG AAGAAGGGAG AGGTGGACAA TGTTCAAAAA 
GTGAAGCAGA TGCAAAGAAT CTGTAGACTT CACTCTCTAC AAGATGAGGG ATCTGGGGAA 
CAGGAGCTCC TGGTAGAACA GAACTGCAAC CAATCAGAGC AAGGAAAGTG GAATTGTCAC 
TTAACGAAGC CTCATTCAAT GAGTAATAAC GAGACAAATT CAGAGCAAGG ATTAACAAAC 
TGTCAGTTGG CAAAGCCACA TCCAAAGAGT TCTAATGAGC AGGATTTAAA AGAAATTTGT 
AAGATATTAT CATCCTCGAA GGACTGGTGC ATCATGCAAG AAGCTCTAGA GAAATGCACA 
CTTCAGTTTA CACCAGGACT TGTGTTGGAA ATTTTGCACA ATTGCAGTAT GCATGGTAAT 
GCTGCATTGA AGTTTTTTTC TTGGTTAGGA GTGCAAACTG GTTATTGCCA CACTAAAGAA 
ACATACAACA TGGCTATGAA AATTTCAGGA CGTGAGAAGG ATTTCAAGCA CATGAGAAGC 
CTTTTTTATG AGATGAGAAG AAAGGGCTGC TTGATAACAC CAGACACATG GGCAATTATG 
ATAATGCAAT ATGGTCGAAC AGGTTTAACA GAGATTTCTC TAAAGACTTT CACTGAAATG 
AAAGATAATG GTTACATTCC AAATGACAGT ACCTACAAGT ATTTGCTCAT CAGTCTTTGT 
GGGAAAAAAG GCAGGAAGGT TGACGAAGCC ATAAAGATAT TCCATGAGAT GATTCGCACA 
AGATATATCC CCGACAGAGA AGTGGTTGGA ACTTATCTCA GTTGTTTATG TGAAGCTGGT 
AGACTATCAG AAGCTAGAAA GAGCACAGAT TCGCTTTGCA GAATTGGTTA CACAATCCCC 
CTGAGTTATT CCTTGTACAT TAGAGCACTT TGTCGATCAG GAAGGTTGGA AGAAGCTTTG 
TCATTGCTGG ATGAAGTAGG GACAGAGCGA TCTACCTTGG ATCAATACAC ATACGGAACT 
CTTGTTCATG GACTACTACG AAAGGGGCGA CAAGAAGAGG CATTGGCTAA AATAGAGTCC 
ATGAAAGAGG CAGGCATTAA TCCAACCGTC CATGTATACA CATCCTTAAT AGTTCATTTC 
TTCAAGGAAA AGCAGATTGA GAAAGCTATG CAAATTTTTG AGAAAATGCA ACAAGATGGC 
TGCGAACCAA CGGTCGTTAC ATACTCGGCG TTGATCCGGG GTTACATGAA CATGGAGAGA 
GCTGATGATG CTTGGAGTGT ATTGAATCAT CTGAAACTAA AAGGACCAAA ACCCGATTTC 
AAGACTTATT CTATGTTCAT TAGTTGTCTA TGTAAGGCTG GGAAATCTGA AGAAGCCTTG 
CAGCTTTTAT CCAGAATGTT GGAGGATGGG ATAGTTCCTA GTACTATCAA CTTCAGAACT 
GTGTTTTTCG GGTTAAATTG TGAAGGTAAA AATGATTTAG CACGGACTGT TATGCAACAG 
AAGTTGGCTT TAAAAAGCAA ACGCAAGTTC TTAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002524089.1
CDS	52..3270
Translation	MRHIIKLHLLGSNSSYKLYSSQLSIHQSFCLSSFSKSSKLSHKQSQKTELQGQSEISTTVTINSLFKEITEILGADNFIPDKHPSGFLISKENHQKHDELNAELKTGTRGIYKNVEPNNVLREEEVFLGLDDTQIRDLNEIDVSPVVREITGIVRAENDLVAMEERLENLGFYFEPEIVEKVLKRCFKVPHLAFRFFSWVKMRDGFCHTTKTYYTMLYIAGEAKEFKVVDDLVEEMEKNSCEKDIKTWSILISQFGKAKLIGKALLFFEKMKTSGCEPDEKIYKMMVHSLCNAGKGEVALAFYKEMVQKDMRLDLSLYKILLISMAKSGDVGAVHLVANDMSRLSQIPEHDVNVCILKSFCVAGKIREALELIRDLKNKDILIDYEYLGTLVKGLCRGDRITDAVEIVEIMKKRNLIDAKIYGIIINGYLRKKDLSKAIELFQRMKESGIQPITSTYTELMQCLFNLNQYDKGFELFNEMLERGIRVDSVATMAIVAAHVRQNHISEAWEVFNTMKDKGANPTWKSYSIFIKELCRVSRTDEILKVLYKMQASKIFINNDIFNLAIAFMEKKGEVDNVQKVKQMQRICRLHSLQDEGSGEQELLVEQNCNQSEQGKWNCHLTKPHSMSNNETNSEQGLTNCQLAKPHPKSSNEQDLKEICKILSSSKDWCIMQEALEKCTLQFTPGLVLEILHNCSMHGNAALKFFSWLGVQTGYCHTKETYNMAMKISGREKDFKHMRSLFYEMRRKGCLITPDTWAIMIMQYGRTGLTEISLKTFTEMKDNGYIPNDSTYKYLLISLCGKKGRKVDEAIKIFHEMIRTRYIPDREVVGTYLSCLCEAGRLSEARKSTDSLCRIGYTIPLSYSLYIRALCRSGRLEEALSLLDEVGTERSTLDQYTYGTLVHGLLRKGRQEEALAKIESMKEAGINPTVHVYTSLIVHFFKEKQIEKAMQIFEKMQQDGCEPTVVTYSALIRGYMNMERADDAWSVLNHLKLKGPKPDFKTYSMFISCLCKAGKSEEALQLLSRMLEDGIVPSTINFRTVFFGLNCEGKNDLARTVMQQKLALKSKRKFLT

Target ORF information:

RefSeq Version	XM_002524043.3
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis putative pentatricopeptide repeat-containing protein At5g06400, mitochondrial (LOC8259784), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002524043.3

ORF Insert Sequence:

ATGAGGCATA TAATCAAACT CCACTTACTG GGTTCAAACT CAAGCTATAA GCTTTACAGT 
TCTCAGCTCT CCATACATCA GAGTTTTTGC CTTTCTAGCT TCTCTAAATC GTCTAAACTT 
TCACATAAGC AATCCCAAAA AACCGAATTG CAAGGCCAAT CAGAAATAAG TACTACTGTT 
ACTATCAATT CACTCTTTAA GGAAATAACT GAGATTTTAG GAGCTGATAA TTTTATCCCA 
GATAAACACC CATCTGGTTT TCTAATATCA AAAGAAAATC ACCAGAAGCA TGATGAGTTA 
AATGCGGAAT TAAAGACTGG CACACGAGGT ATTTATAAAA ATGTCGAGCC GAACAATGTG 
TTGCGAGAGG AGGAGGTTTT TCTGGGTTTG GATGATACCC AGATAAGGGA TTTGAATGAA 
ATTGATGTTA GCCCTGTCGT TCGTGAGATT ACTGGGATTG TTCGCGCAGA AAATGACCTG 
GTTGCGATGG AGGAGAGGTT AGAGAATCTG GGGTTTTATT TTGAACCTGA GATCGTTGAA 
AAAGTATTGA AAAGATGCTT TAAAGTGCCA CATTTAGCTT TTAGGTTTTT CAGTTGGGTG 
AAGATGAGAG ATGGGTTTTG TCATACAACC AAGACTTATT ATACCATGCT GTATATAGCT 
GGGGAAGCTA AAGAATTTAA GGTAGTGGAT GATTTGGTAG AGGAAATGGA AAAGAACTCT 
TGCGAAAAGG ATATCAAGAC ATGGAGTATC CTTATCTCAC AATTTGGAAA GGCAAAGTTG 
ATTGGCAAAG CCTTGTTGTT TTTTGAGAAG ATGAAGACAT CTGGGTGTGA ACCCGATGAA 
AAAATTTATA AAATGATGGT GCATTCTCTT TGTAATGCTG GGAAAGGTGA GGTTGCATTG 
GCATTTTACA AGGAGATGGT ACAAAAGGAC ATGAGACTGG ATTTAAGTTT GTACAAAATA 
TTACTCATTA GCATGGCAAA GTCGGGAGAT GTTGGCGCTG TTCACTTGGT TGCAAATGAC 
ATGAGTAGGC TTTCACAGAT TCCGGAACAT GATGTTAATG TTTGCATACT GAAGAGTTTT 
TGTGTGGCTG GGAAAATTAG AGAAGCTCTA GAATTGATTC GTGACCTCAA GAACAAAGAT 
ATACTAATTG ACTATGAATA TCTTGGGACT TTAGTGAAAG GTCTGTGCAG GGGTGATAGG 
ATTACTGATG CTGTGGAAAT TGTTGAAATT ATGAAGAAAA GAAATCTTAT TGATGCAAAG 
ATTTATGGGA TTATCATAAA TGGATACTTG AGAAAAAAAG ACCTTTCAAA GGCAATTGAG 
CTATTTCAGA GAATGAAAGA ATCTGGTATT CAACCAATTA CTTCCACCTA TACAGAGTTA 
ATGCAATGCC TGTTCAATTT GAACCAGTAT GACAAAGGCT TTGAGCTATT TAACGAGATG 
CTGGAAAGAG GAATTAGGGT TGATAGTGTG GCAACTATGG CAATTGTTGC CGCTCATGTT 
CGTCAAAACC ATATATCAGA AGCATGGGAA GTGTTCAATA CCATGAAGGA TAAGGGAGCC 
AATCCTACTT GGAAATCTTA TTCCATATTC ATTAAGGAGC TTTGCAGAGT TTCAAGAACA 
GATGAGATTT TGAAAGTATT GTACAAAATG CAGGCCTCCA AGATATTCAT TAATAATGAT 
ATATTCAACT TAGCGATAGC TTTTATGGAG AAGAAGGGAG AGGTGGACAA TGTTCAAAAA 
GTGAAGCAGA TGCAAAGAAT CTGTAGACTT CACTCTCTAC AAGATGAGGG ATCTGGGGAA 
CAGGAGCTCC TGGTAGAACA GAACTGCAAC CAATCAGAGC AAGGAAAGTG GAATTGTCAC 
TTAACGAAGC CTCATTCAAT GAGTAATAAC GAGACAAATT CAGAGCAAGG ATTAACAAAC 
TGTCAGTTGG CAAAGCCACA TCCAAAGAGT TCTAATGAGC AGGATTTAAA AGAAATTTGT 
AAGATATTAT CATCCTCGAA GGACTGGTGC ATCATGCAAG AAGCTCTAGA GAAATGCACA 
CTTCAGTTTA CACCAGGACT TGTGTTGGAA ATTTTGCACA ATTGCAGTAT GCATGGTAAT 
GCTGCATTGA AGTTTTTTTC TTGGTTAGGA GTGCAAACTG GTTATTGCCA CACTAAAGAA 
ACATACAACA TGGCTATGAA AATTTCAGGA CGTGAGAAGG ATTTCAAGCA CATGAGAAGC 
CTTTTTTATG AGATGAGAAG AAAGGGCTGC TTGATAACAC CAGACACATG GGCAATTATG 
ATAATGCAAT ATGGTCGAAC AGGTTTAACA GAGATTTCTC TAAAGACTTT CACTGAAATG 
AAAGATAATG GTTACATTCC AAATGACAGT ACCTACAAGT ATTTGCTCAT CAGTCTTTGT 
GGGAAAAAAG GCAGGAAGGT TGACGAAGCC ATAAAGATAT TCCATGAGAT GATTCGCACA 
AGATATATCC CCGACAGAGA AGTGGTTGGA ACTTATCTCA GTTGTTTATG TGAAGCTGGT 
AGACTATCAG AAGCTAGAAA GAGCACAGAT TCGCTTTGCA GAATTGGTTA CACAATCCCC 
CTGAGTTATT CCTTGTACAT TAGAGCACTT TGTCGATCAG GAAGGTTGGA AGAAGCTTTG 
TCATTGCTGG ATGAAGTAGG GACAGAGCGA TCTACCTTGG ATCAATACAC ATACGGAACT 
CTTGTTCATG GACTACTACG AAAGGGGCGA CAAGAAGAGG CATTGGCTAA AATAGAGTCC 
ATGAAAGAGG CAGGCATTAA TCCAACCGTC CATGTATACA CATCCTTAAT AGTTCATTTC 
TTCAAGGAAA AGCAGATTGA GAAAGCTATG CAAATTTTTG AGAAAATGCA ACAAGATGGC 
TGCGAACCAA CGGTCGTTAC ATACTCGGCG TTGATCCGGG GTTACATGAA CATGGAGAGA 
GCTGATGATG CTTGGAGTGT ATTGAATCAT CTGAAACTAA AAGGACCAAA ACCCGATTTC 
AAGACTTATT CTATGTTCAT TAGTTGTCTA TGTAAGGCTG GGAAATCTGA AGAAGCCTTG 
CAGCTTTTAT CCAGAATGTT GGAGGATGGG ATAGTTCCTA GTACTATCAA CTTCAGAACT 
GTGTTTTTCG GGTTAAATTG TGAAGGTAAA AATGATTTAG CACGGACTGT TATGCAACAG 
AAGTTGGCTT TAAAAAGCAA ACGCAAGTTC TTAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8259784 ( XM_002524043.2 ) cDNA ORF clone, Ricinus communis(castor bean) -> XP_002524089.1 Ricinus communis putative pentatricopeptide repeat-containing protein At5g06400, mitochondrial (LOC8259784), mRNA.

Price & Availability↑

CloneID	ORb01656
Clone ID Related Accession (Same CDS sequence)	XM_002524043.3 , XM_002524043.2
Accession Version	XM_002524043.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1456243200000
Organism	Ricinus communis(castor bean)
Product	putative pentatricopeptide repeat-containing protein At5g06400, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002994432.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 25, 2016 this sequence version replaced XM_002524043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ricinus communis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCATA TAATCAAACT CCACTTACTG GGTTCAAACT CAAGCTATAA GCTTTACAGT 
TCTCAGCTCT CCATACATCA GAGTTTTTGC CTTTCTAGCT TCTCTAAATC GTCTAAACTT 
TCACATAAGC AATCCCAAAA AACCGAATTG CAAGGCCAAT CAGAAATAAG TACTACTGTT 
ACTATCAATT CACTCTTTAA GGAAATAACT GAGATTTTAG GAGCTGATAA TTTTATCCCA 
GATAAACACC CATCTGGTTT TCTAATATCA AAAGAAAATC ACCAGAAGCA TGATGAGTTA 
AATGCGGAAT TAAAGACTGG CACACGAGGT ATTTATAAAA ATGTCGAGCC GAACAATGTG 
TTGCGAGAGG AGGAGGTTTT TCTGGGTTTG GATGATACCC AGATAAGGGA TTTGAATGAA 
ATTGATGTTA GCCCTGTCGT TCGTGAGATT ACTGGGATTG TTCGCGCAGA AAATGACCTG 
GTTGCGATGG AGGAGAGGTT AGAGAATCTG GGGTTTTATT TTGAACCTGA GATCGTTGAA 
AAAGTATTGA AAAGATGCTT TAAAGTGCCA CATTTAGCTT TTAGGTTTTT CAGTTGGGTG 
AAGATGAGAG ATGGGTTTTG TCATACAACC AAGACTTATT ATACCATGCT GTATATAGCT 
GGGGAAGCTA AAGAATTTAA GGTAGTGGAT GATTTGGTAG AGGAAATGGA AAAGAACTCT 
TGCGAAAAGG ATATCAAGAC ATGGAGTATC CTTATCTCAC AATTTGGAAA GGCAAAGTTG 
ATTGGCAAAG CCTTGTTGTT TTTTGAGAAG ATGAAGACAT CTGGGTGTGA ACCCGATGAA 
AAAATTTATA AAATGATGGT GCATTCTCTT TGTAATGCTG GGAAAGGTGA GGTTGCATTG 
GCATTTTACA AGGAGATGGT ACAAAAGGAC ATGAGACTGG ATTTAAGTTT GTACAAAATA 
TTACTCATTA GCATGGCAAA GTCGGGAGAT GTTGGCGCTG TTCACTTGGT TGCAAATGAC 
ATGAGTAGGC TTTCACAGAT TCCGGAACAT GATGTTAATG TTTGCATACT GAAGAGTTTT 
TGTGTGGCTG GGAAAATTAG AGAAGCTCTA GAATTGATTC GTGACCTCAA GAACAAAGAT 
ATACTAATTG ACTATGAATA TCTTGGGACT TTAGTGAAAG GTCTGTGCAG GGGTGATAGG 
ATTACTGATG CTGTGGAAAT TGTTGAAATT ATGAAGAAAA GAAATCTTAT TGATGCAAAG 
ATTTATGGGA TTATCATAAA TGGATACTTG AGAAAAAAAG ACCTTTCAAA GGCAATTGAG 
CTATTTCAGA GAATGAAAGA ATCTGGTATT CAACCAATTA CTTCCACCTA TACAGAGTTA 
ATGCAATGCC TGTTCAATTT GAACCAGTAT GACAAAGGCT TTGAGCTATT TAACGAGATG 
CTGGAAAGAG GAATTAGGGT TGATAGTGTG GCAACTATGG CAATTGTTGC CGCTCATGTT 
CGTCAAAACC ATATATCAGA AGCATGGGAA GTGTTCAATA CCATGAAGGA TAAGGGAGCC 
AATCCTACTT GGAAATCTTA TTCCATATTC ATTAAGGAGC TTTGCAGAGT TTCAAGAACA 
GATGAGATTT TGAAAGTATT GTACAAAATG CAGGCCTCCA AGATATTCAT TAATAATGAT 
ATATTCAACT TAGCGATAGC TTTTATGGAG AAGAAGGGAG AGGTGGACAA TGTTCAAAAA 
GTGAAGCAGA TGCAAAGAAT CTGTAGACTT CACTCTCTAC AAGATGAGGG ATCTGGGGAA 
CAGGAGCTCC TGGTAGAACA GAACTGCAAC CAATCAGAGC AAGGAAAGTG GAATTGTCAC 
TTAACGAAGC CTCATTCAAT GAGTAATAAC GAGACAAATT CAGAGCAAGG ATTAACAAAC 
TGTCAGTTGG CAAAGCCACA TCCAAAGAGT TCTAATGAGC AGGATTTAAA AGAAATTTGT 
AAGATATTAT CATCCTCGAA GGACTGGTGC ATCATGCAAG AAGCTCTAGA GAAATGCACA 
CTTCAGTTTA CACCAGGACT TGTGTTGGAA ATTTTGCACA ATTGCAGTAT GCATGGTAAT 
GCTGCATTGA AGTTTTTTTC TTGGTTAGGA GTGCAAACTG GTTATTGCCA CACTAAAGAA 
ACATACAACA TGGCTATGAA AATTTCAGGA CGTGAGAAGG ATTTCAAGCA CATGAGAAGC 
CTTTTTTATG AGATGAGAAG AAAGGGCTGC TTGATAACAC CAGACACATG GGCAATTATG 
ATAATGCAAT ATGGTCGAAC AGGTTTAACA GAGATTTCTC TAAAGACTTT CACTGAAATG 
AAAGATAATG GTTACATTCC AAATGACAGT ACCTACAAGT ATTTGCTCAT CAGTCTTTGT 
GGGAAAAAAG GCAGGAAGGT TGACGAAGCC ATAAAGATAT TCCATGAGAT GATTCGCACA 
AGATATATCC CCGACAGAGA AGTGGTTGGA ACTTATCTCA GTTGTTTATG TGAAGCTGGT 
AGACTATCAG AAGCTAGAAA GAGCACAGAT TCGCTTTGCA GAATTGGTTA CACAATCCCC 
CTGAGTTATT CCTTGTACAT TAGAGCACTT TGTCGATCAG GAAGGTTGGA AGAAGCTTTG 
TCATTGCTGG ATGAAGTAGG GACAGAGCGA TCTACCTTGG ATCAATACAC ATACGGAACT 
CTTGTTCATG GACTACTACG AAAGGGGCGA CAAGAAGAGG CATTGGCTAA AATAGAGTCC 
ATGAAAGAGG CAGGCATTAA TCCAACCGTC CATGTATACA CATCCTTAAT AGTTCATTTC 
TTCAAGGAAA AGCAGATTGA GAAAGCTATG CAAATTTTTG AGAAAATGCA ACAAGATGGC 
TGCGAACCAA CGGTCGTTAC ATACTCGGCG TTGATCCGGG GTTACATGAA CATGGAGAGA 
GCTGATGATG CTTGGAGTGT ATTGAATCAT CTGAAACTAA AAGGACCAAA ACCCGATTTC 
AAGACTTATT CTATGTTCAT TAGTTGTCTA TGTAAGGCTG GGAAATCTGA AGAAGCCTTG 
CAGCTTTTAT CCAGAATGTT GGAGGATGGG ATAGTTCCTA GTACTATCAA CTTCAGAACT 
GTGTTTTTCG GGTTAAATTG TGAAGGTAAA AATGATTTAG CACGGACTGT TATGCAACAG 
AAGTTGGCTT TAAAAAGCAA ACGCAAGTTC TTAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002524089.1
CDS	36..3254
Translation	MRHIIKLHLLGSNSSYKLYSSQLSIHQSFCLSSFSKSSKLSHKQSQKTELQGQSEISTTVTINSLFKEITEILGADNFIPDKHPSGFLISKENHQKHDELNAELKTGTRGIYKNVEPNNVLREEEVFLGLDDTQIRDLNEIDVSPVVREITGIVRAENDLVAMEERLENLGFYFEPEIVEKVLKRCFKVPHLAFRFFSWVKMRDGFCHTTKTYYTMLYIAGEAKEFKVVDDLVEEMEKNSCEKDIKTWSILISQFGKAKLIGKALLFFEKMKTSGCEPDEKIYKMMVHSLCNAGKGEVALAFYKEMVQKDMRLDLSLYKILLISMAKSGDVGAVHLVANDMSRLSQIPEHDVNVCILKSFCVAGKIREALELIRDLKNKDILIDYEYLGTLVKGLCRGDRITDAVEIVEIMKKRNLIDAKIYGIIINGYLRKKDLSKAIELFQRMKESGIQPITSTYTELMQCLFNLNQYDKGFELFNEMLERGIRVDSVATMAIVAAHVRQNHISEAWEVFNTMKDKGANPTWKSYSIFIKELCRVSRTDEILKVLYKMQASKIFINNDIFNLAIAFMEKKGEVDNVQKVKQMQRICRLHSLQDEGSGEQELLVEQNCNQSEQGKWNCHLTKPHSMSNNETNSEQGLTNCQLAKPHPKSSNEQDLKEICKILSSSKDWCIMQEALEKCTLQFTPGLVLEILHNCSMHGNAALKFFSWLGVQTGYCHTKETYNMAMKISGREKDFKHMRSLFYEMRRKGCLITPDTWAIMIMQYGRTGLTEISLKTFTEMKDNGYIPNDSTYKYLLISLCGKKGRKVDEAIKIFHEMIRTRYIPDREVVGTYLSCLCEAGRLSEARKSTDSLCRIGYTIPLSYSLYIRALCRSGRLEEALSLLDEVGTERSTLDQYTYGTLVHGLLRKGRQEEALAKIESMKEAGINPTVHVYTSLIVHFFKEKQIEKAMQIFEKMQQDGCEPTVVTYSALIRGYMNMERADDAWSVLNHLKLKGPKPDFKTYSMFISCLCKAGKSEEALQLLSRMLEDGIVPSTINFRTVFFGLNCEGKNDLARTVMQQKLALKSKRKFLT

Target ORF information:

RefSeq Version	XM_002524043.2
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis putative pentatricopeptide repeat-containing protein At5g06400, mitochondrial (LOC8259784), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002524043.2

ORF Insert Sequence:

ATGAGGCATA TAATCAAACT CCACTTACTG GGTTCAAACT CAAGCTATAA GCTTTACAGT 
TCTCAGCTCT CCATACATCA GAGTTTTTGC CTTTCTAGCT TCTCTAAATC GTCTAAACTT 
TCACATAAGC AATCCCAAAA AACCGAATTG CAAGGCCAAT CAGAAATAAG TACTACTGTT 
ACTATCAATT CACTCTTTAA GGAAATAACT GAGATTTTAG GAGCTGATAA TTTTATCCCA 
GATAAACACC CATCTGGTTT TCTAATATCA AAAGAAAATC ACCAGAAGCA TGATGAGTTA 
AATGCGGAAT TAAAGACTGG CACACGAGGT ATTTATAAAA ATGTCGAGCC GAACAATGTG 
TTGCGAGAGG AGGAGGTTTT TCTGGGTTTG GATGATACCC AGATAAGGGA TTTGAATGAA 
ATTGATGTTA GCCCTGTCGT TCGTGAGATT ACTGGGATTG TTCGCGCAGA AAATGACCTG 
GTTGCGATGG AGGAGAGGTT AGAGAATCTG GGGTTTTATT TTGAACCTGA GATCGTTGAA 
AAAGTATTGA AAAGATGCTT TAAAGTGCCA CATTTAGCTT TTAGGTTTTT CAGTTGGGTG 
AAGATGAGAG ATGGGTTTTG TCATACAACC AAGACTTATT ATACCATGCT GTATATAGCT 
GGGGAAGCTA AAGAATTTAA GGTAGTGGAT GATTTGGTAG AGGAAATGGA AAAGAACTCT 
TGCGAAAAGG ATATCAAGAC ATGGAGTATC CTTATCTCAC AATTTGGAAA GGCAAAGTTG 
ATTGGCAAAG CCTTGTTGTT TTTTGAGAAG ATGAAGACAT CTGGGTGTGA ACCCGATGAA 
AAAATTTATA AAATGATGGT GCATTCTCTT TGTAATGCTG GGAAAGGTGA GGTTGCATTG 
GCATTTTACA AGGAGATGGT ACAAAAGGAC ATGAGACTGG ATTTAAGTTT GTACAAAATA 
TTACTCATTA GCATGGCAAA GTCGGGAGAT GTTGGCGCTG TTCACTTGGT TGCAAATGAC 
ATGAGTAGGC TTTCACAGAT TCCGGAACAT GATGTTAATG TTTGCATACT GAAGAGTTTT 
TGTGTGGCTG GGAAAATTAG AGAAGCTCTA GAATTGATTC GTGACCTCAA GAACAAAGAT 
ATACTAATTG ACTATGAATA TCTTGGGACT TTAGTGAAAG GTCTGTGCAG GGGTGATAGG 
ATTACTGATG CTGTGGAAAT TGTTGAAATT ATGAAGAAAA GAAATCTTAT TGATGCAAAG 
ATTTATGGGA TTATCATAAA TGGATACTTG AGAAAAAAAG ACCTTTCAAA GGCAATTGAG 
CTATTTCAGA GAATGAAAGA ATCTGGTATT CAACCAATTA CTTCCACCTA TACAGAGTTA 
ATGCAATGCC TGTTCAATTT GAACCAGTAT GACAAAGGCT TTGAGCTATT TAACGAGATG 
CTGGAAAGAG GAATTAGGGT TGATAGTGTG GCAACTATGG CAATTGTTGC CGCTCATGTT 
CGTCAAAACC ATATATCAGA AGCATGGGAA GTGTTCAATA CCATGAAGGA TAAGGGAGCC 
AATCCTACTT GGAAATCTTA TTCCATATTC ATTAAGGAGC TTTGCAGAGT TTCAAGAACA 
GATGAGATTT TGAAAGTATT GTACAAAATG CAGGCCTCCA AGATATTCAT TAATAATGAT 
ATATTCAACT TAGCGATAGC TTTTATGGAG AAGAAGGGAG AGGTGGACAA TGTTCAAAAA 
GTGAAGCAGA TGCAAAGAAT CTGTAGACTT CACTCTCTAC AAGATGAGGG ATCTGGGGAA 
CAGGAGCTCC TGGTAGAACA GAACTGCAAC CAATCAGAGC AAGGAAAGTG GAATTGTCAC 
TTAACGAAGC CTCATTCAAT GAGTAATAAC GAGACAAATT CAGAGCAAGG ATTAACAAAC 
TGTCAGTTGG CAAAGCCACA TCCAAAGAGT TCTAATGAGC AGGATTTAAA AGAAATTTGT 
AAGATATTAT CATCCTCGAA GGACTGGTGC ATCATGCAAG AAGCTCTAGA GAAATGCACA 
CTTCAGTTTA CACCAGGACT TGTGTTGGAA ATTTTGCACA ATTGCAGTAT GCATGGTAAT 
GCTGCATTGA AGTTTTTTTC TTGGTTAGGA GTGCAAACTG GTTATTGCCA CACTAAAGAA 
ACATACAACA TGGCTATGAA AATTTCAGGA CGTGAGAAGG ATTTCAAGCA CATGAGAAGC 
CTTTTTTATG AGATGAGAAG AAAGGGCTGC TTGATAACAC CAGACACATG GGCAATTATG 
ATAATGCAAT ATGGTCGAAC AGGTTTAACA GAGATTTCTC TAAAGACTTT CACTGAAATG 
AAAGATAATG GTTACATTCC AAATGACAGT ACCTACAAGT ATTTGCTCAT CAGTCTTTGT 
GGGAAAAAAG GCAGGAAGGT TGACGAAGCC ATAAAGATAT TCCATGAGAT GATTCGCACA 
AGATATATCC CCGACAGAGA AGTGGTTGGA ACTTATCTCA GTTGTTTATG TGAAGCTGGT 
AGACTATCAG AAGCTAGAAA GAGCACAGAT TCGCTTTGCA GAATTGGTTA CACAATCCCC 
CTGAGTTATT CCTTGTACAT TAGAGCACTT TGTCGATCAG GAAGGTTGGA AGAAGCTTTG 
TCATTGCTGG ATGAAGTAGG GACAGAGCGA TCTACCTTGG ATCAATACAC ATACGGAACT 
CTTGTTCATG GACTACTACG AAAGGGGCGA CAAGAAGAGG CATTGGCTAA AATAGAGTCC 
ATGAAAGAGG CAGGCATTAA TCCAACCGTC CATGTATACA CATCCTTAAT AGTTCATTTC 
TTCAAGGAAA AGCAGATTGA GAAAGCTATG CAAATTTTTG AGAAAATGCA ACAAGATGGC 
TGCGAACCAA CGGTCGTTAC ATACTCGGCG TTGATCCGGG GTTACATGAA CATGGAGAGA 
GCTGATGATG CTTGGAGTGT ATTGAATCAT CTGAAACTAA AAGGACCAAA ACCCGATTTC 
AAGACTTATT CTATGTTCAT TAGTTGTCTA TGTAAGGCTG GGAAATCTGA AGAAGCCTTG 
CAGCTTTTAT CCAGAATGTT GGAGGATGGG ATAGTTCCTA GTACTATCAA CTTCAGAACT 
GTGTTTTTCG GGTTAAATTG TGAAGGTAAA AATGATTTAG CACGGACTGT TATGCAACAG 
AAGTTGGCTT TAAAAAGCAA ACGCAAGTTC TTAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8259784 cDNA ORF clone, Ricinus communis(castor bean)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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