LOC685590 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC685590
Entrez Gene ID	685590
Full Name	similar to Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PI3-kinase p110 subunit alpha) (PtdIns-3-kinase p110) (PI3K)
General protein information	Preferred Names similar to Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PI3-kinase p110 subunit alpha) (PtdIns-3-kinase p110) (PI3K) Names LOW QUALITY PROTEIN: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq13

mRNA and Protein(s)

mRNA	Protein	Name
XM_017602181.1	XP_017457670.1	LOW QUALITY PROTEIN: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like
XM_017588100.1	XP_017443589.1	LOW QUALITY PROTEIN: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like

Pathways from BioSystems
Gene Ontology

The following LOC685590 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC685590 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa56111	XM_017588100.1 Latest version!	Rattus norvegicus similar to Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PI3-kinase p110 subunit alpha) (PtdIns-3-kinase p110) (PI3K) (LOC685590), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa56111	XM_017602181.1 Latest version!	Rattus norvegicus similar to Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PI3-kinase p110 subunit alpha) (PtdIns-3-kinase p110) (PI3K) (LOC685590), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC685590 ( XM_017602181.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457670.1 Rattus norvegicus similar to Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PI3-kinase p110 subunit alpha) (PtdIns-3-kinase p110) (PI3K) (LOC685590), mRNA.

Price & Availability↑

CloneID	ORa56111
Clone ID Related Accession (Same CDS sequence)	XM_017602181.1 , XM_017588100.1
Accession Version	XM_017602181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	LOW QUALITY PROTEIN: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases frameshifts :: corrected 2 indels internal stop codons :: corrected 1 genomic stop codons ##RefSeq-Attributes-END##

ATGTGCATGC AGATGTCCAT GGAAAAGTTT CAGGCAGTTG TGAATTGCCC AGTTTTGCAG 
AGGGTCAGAA CAATTCCTCC ACGACCATCT TCGGGTGAAC TGTGGGGCAT CCACTTGATG 
CCCCCACGAA TCCTAGTGGA ATGTTTACTC CCAAATGGAA TGATAGTGAC TTTAGAATGC 
CTCCGTGAGG CCACACTAGT CACCATCAAG CATGAACTGT TCAAAGAGGC CAGGAAATAC 
CCTCTCCATC AGCTTCTGCA AGATGAATCA TCTTACATTT TCGTAAGTGT TACCCAAGAA 
GCAGAAAGGG AAGAATTTTT CGATGAAACA AGACGGCTTT GTGACCTTCG GCTTTTTCAA 
CCCTTTTTAA AAGTAATTGA GCCAGTAGGC AACCGTGAAG AAAAGATCCT CAACCGAGAA 
ATTGGTTTTG TTATTGGCAT GCCAGTGTGT GAATTTGATA TGGTTAAAGA TCTAGAAGTC 
CAAGACTTCC GANAGAACAT TCTGAATGTT TGCAAAGAAG CCGTGGGCCT GCGGGATCTC 
AACTCGCCTC ATAGCAGAGC AATGTATGTC TACCCTCCAA ATGTCGAGTC TTCCCCAGAA 
CTGCCAAAGC ACATCTACAA CAAGTTAGAT AAAGGACAAA TCATAGTGGT GATTTGGGTG 
ATAGTCTCTC CAAACAACGA CAAGCAGAAG TACACTCTGA AGATCAACCA TGACTGCGTG 
CCAGAGCAAG TCATTGCTGA GGCCATCAGG AAGAAGACCC GGAGCATGTT GCTGTCCTCG 
GAGCAGCTGA AACTCTGTGT CTTAGAATAC CAGGGCAAGT ACATTCTCAA AGTGTGTGGC 
TGTGATGAGT ACTTCCTAGA GAAGTACCCT CTGAGTCAGT ACAAGTACAT AAGAAGCTGT 
ATAATGCTGG GGAGGATGCC CAACTTGATG CTGATGGCCA AGGAGAGCCT GCACTCTCAG 
CTGCCGATCG ATAGCTTCAC AATGCCATCC TACTCCAGGC GCATTTCCAC AGCGACACCC 
TATATGAACG GGGAGACTGC TACGAAATCT CTCTGGGTTA TAAATAGCGC GCTCAGAATA 
AAAATTCTGT GTGCAACCTA TGTAAATGTA AATATTCGAG ACATTGATAA GATCTATGTT 
CGAACAGGTA TCTACCATGG AGGAGAATCC TTATGTGACA ATGTGAATAC TCAAAGAGTC 
CCTTGTTCCA ATCCTAGGTG GAATGAATGG CTGAATTATG ATATATACAT TCCTGATCTT 
CCTCGTGCTG CCCGCCTTTG CCTTTCAATC TGCTCTGTTA AAGGCCGAAA GGGTGCTAAG 
GAGGAGCACT GTCCGTTGGC CTGGGGAAAC ATAAACTTGT TTGATTATAC AGACACCCTA 
GTGTCCGGGA AAATGGCTTT GAATCTCTGG CCTGTACCAC ATGGGTTGGA AGATCTGCTG 
AACCCTATTG GTGTTACTGG GTCAAATCCA AATAAAGAAA CTCCATGCTT AGAGTTGGAG 
TTTGATTGGT TCAGCAGTGT GGTGAAGTTT CCAGATATGT CTGTGATCGA AGAGCATGCC 
AATTGGTCTG TGTCCCGAGA AGCCGGATTC AGTTACTCTC ATACAGGACT GAGTAACAGA 
CTAGCCAGAG ACAATGAGTT AAGAGAAAAT GACAAGGAAC AGCTCTGAGC ACTTTGTACC 
CGGGACCCAC TGTCTGAAAT CACTGAACAA GAGAAAGACT TCCTATGGAG CCACAGACAC 
TACTGTGTAA CTATTCCTGA AATCCTACCC AAATTGCTTC TGTCTGTCAA GTGGAATTCC 
AGAGATGAAG TGGCCCAGAT GTACTGCTTA GTAAAAGATT GGCCTCCAAT CAAACCAGAG 
CAAGCCATGG AGCTCCTGGA CTGTAACTAC CCAGACCCCA TGGTTCGGAG CTTTGCTGTC 
CGGTGCTTGG AAAAATACTT AACAGATGAC AAACTTTCTC AGTACCTCAT CCAGCTTGTA 
CAGGTCTTAA AATATGAACA GTATTTGGAT AACCTGCTTG TGAGATTTTT ACTCAAGAAA 
GCACTGACAA ATCAAAGGAT TGGCCATTTT TTCTTTTGGC ATTTAAAATC TGAGATGCAC 
AATAAGACTG TCAGTCAGAG GTTCAGCCTG CTGTTGGAGT CCTACTGCCG TGCCTGTGGG 
ATGTATCTGA AGCACCTGAA CAGACAGGTA GAGGCCATGG AGAAGCTCAT CAATCTAACT 
GACATCCTCA AGCAAGAGAA GAAGGATGAG ACACAGAAGG TACAGATGAA GTTCTTGGTT 
GAACAGATGA GACAGCCAGA TTTCATGGAT GCTTTGCAGG GTTTTCTGTC CCCTCTAAAT 
CCTGCTCATC AACTAGGAAA CCTCAGGCTT GAAGAGTGTC GAATTATGTC CTCTGCAAAA 
AGGCCACTGT GGTTGAATTG GGAGAACCCA GACATCATGT CAGAGCTACT GTTTCAGAAC 
AATGAGATCA TCTTTAAAAA TGGCGATGAC TTACGGCAAG ACATGTTAAC CCTTNNCAGA 
ATCATGGAGA ACATCTGGCA AAACCAAGGC CTTGACCTTC GCATGCTACC TTATGCCTGT 
CTATCCATTG GGGACTGTGT GGGTCTCATC GAGGTGGTGA GAAACTCTCA CACCATCATG 
CAGATTCAGT GCAAAGGAGG CCTGAAGGGG GCACTGCAGT TCAACAGGCA CACGCTGCAT 
CAGTGGCTCA AGGACAAGAA CAAGGGCAAG ATATATGACG CAGCCATTGA CCTGTTCACT 
TGGTCCTGCG CTGGGTACTG CGTGGCAACC TTTATCTTGG GAATTGGAGA CCGGCACAAC 
AGCAACATCA TGGTGAAAGA TGACGGACAG CTGTTTCATA TAGATTTTGG GCACTTTTTG 
GATCACAAGA AGAAAAAATT TGGCTATAAA CGGGAACGCG TGCCGTTTGT TTTGACGCAG 
GATTTCTTAA TAGTGATTAG TAAAGGAGCA CAAGAGTACA CAAAGACCAG AGAGTTTGAG 
AGGTTTCAGG AGATGTGTTA CAAGGCGTAC CTAGCAATTC GGCAGCATGC CAATCTCTTC 
ATCAACCTTT TCTCCATGAT GCTTGGCTCC GGAATGCCAG AACTGCAGTC TTTTGATGAT 
ATTGCATATA TTCGAAAGAC TCTAGCCTTA GACAAAACTG AGCAAGAGGC TCTGGAGTAT 
TTCACAAAGC AAATGAATGA CGCACATCAT GGTGGCTGGA CAACAAAAAT GGACTGGATC 
TTCCACACCA TCAAGCAGCA TGCATTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457670.1
CDS	1..3273
Translation	MCMQMSMEKFQAVVNCPVLQRVRTIPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLVTIKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFVIGMPVCEFDMVKDLEVQDFRnullNILNVCKEAVGLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLHSQLPIDSFTMPSYSRRISTATPYMNGETATKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGESLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHTGLSNRLARDNELRENDKEQL*ALCTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRSFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFSLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRQPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLnullRIMENIWQNQGLDLRMLPYACLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNRHTLHQWLKDKNKGKIYDAAIDLFTWSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQEYTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFTKQMNDAHHGGWTTKMDWIFHTIKQHALN

Target ORF information:

RefSeq Version	XM_017602181.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus similar to Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PI3-kinase p110 subunit alpha) (PtdIns-3-kinase p110) (PI3K) (LOC685590), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017602181.1

ORF Insert Sequence:

ATGTGCATGC AGATGTCCAT GGAAAAGTTT CAGGCAGTTG TGAATTGCCC AGTTTTGCAG 
AGGGTCAGAA CAATTCCTCC ACGACCATCT TCGGGTGAAC TGTGGGGCAT CCACTTGATG 
CCCCCACGAA TCCTAGTGGA ATGTTTACTC CCAAATGGAA TGATAGTGAC TTTAGAATGC 
CTCCGTGAGG CCACACTAGT CACCATCAAG CATGAACTGT TCAAAGAGGC CAGGAAATAC 
CCTCTCCATC AGCTTCTGCA AGATGAATCA TCTTACATTT TCGTAAGTGT TACCCAAGAA 
GCAGAAAGGG AAGAATTTTT CGATGAAACA AGACGGCTTT GTGACCTTCG GCTTTTTCAA 
CCCTTTTTAA AAGTAATTGA GCCAGTAGGC AACCGTGAAG AAAAGATCCT CAACCGAGAA 
ATTGGTTTTG TTATTGGCAT GCCAGTGTGT GAATTTGATA TGGTTAAAGA TCTAGAAGTC 
CAAGACTTCC GANAGAACAT TCTGAATGTT TGCAAAGAAG CCGTGGGCCT GCGGGATCTC 
AACTCGCCTC ATAGCAGAGC AATGTATGTC TACCCTCCAA ATGTCGAGTC TTCCCCAGAA 
CTGCCAAAGC ACATCTACAA CAAGTTAGAT AAAGGACAAA TCATAGTGGT GATTTGGGTG 
ATAGTCTCTC CAAACAACGA CAAGCAGAAG TACACTCTGA AGATCAACCA TGACTGCGTG 
CCAGAGCAAG TCATTGCTGA GGCCATCAGG AAGAAGACCC GGAGCATGTT GCTGTCCTCG 
GAGCAGCTGA AACTCTGTGT CTTAGAATAC CAGGGCAAGT ACATTCTCAA AGTGTGTGGC 
TGTGATGAGT ACTTCCTAGA GAAGTACCCT CTGAGTCAGT ACAAGTACAT AAGAAGCTGT 
ATAATGCTGG GGAGGATGCC CAACTTGATG CTGATGGCCA AGGAGAGCCT GCACTCTCAG 
CTGCCGATCG ATAGCTTCAC AATGCCATCC TACTCCAGGC GCATTTCCAC AGCGACACCC 
TATATGAACG GGGAGACTGC TACGAAATCT CTCTGGGTTA TAAATAGCGC GCTCAGAATA 
AAAATTCTGT GTGCAACCTA TGTAAATGTA AATATTCGAG ACATTGATAA GATCTATGTT 
CGAACAGGTA TCTACCATGG AGGAGAATCC TTATGTGACA ATGTGAATAC TCAAAGAGTC 
CCTTGTTCCA ATCCTAGGTG GAATGAATGG CTGAATTATG ATATATACAT TCCTGATCTT 
CCTCGTGCTG CCCGCCTTTG CCTTTCAATC TGCTCTGTTA AAGGCCGAAA GGGTGCTAAG 
GAGGAGCACT GTCCGTTGGC CTGGGGAAAC ATAAACTTGT TTGATTATAC AGACACCCTA 
GTGTCCGGGA AAATGGCTTT GAATCTCTGG CCTGTACCAC ATGGGTTGGA AGATCTGCTG 
AACCCTATTG GTGTTACTGG GTCAAATCCA AATAAAGAAA CTCCATGCTT AGAGTTGGAG 
TTTGATTGGT TCAGCAGTGT GGTGAAGTTT CCAGATATGT CTGTGATCGA AGAGCATGCC 
AATTGGTCTG TGTCCCGAGA AGCCGGATTC AGTTACTCTC ATACAGGACT GAGTAACAGA 
CTAGCCAGAG ACAATGAGTT AAGAGAAAAT GACAAGGAAC AGCTCTGAGC ACTTTGTACC 
CGGGACCCAC TGTCTGAAAT CACTGAACAA GAGAAAGACT TCCTATGGAG CCACAGACAC 
TACTGTGTAA CTATTCCTGA AATCCTACCC AAATTGCTTC TGTCTGTCAA GTGGAATTCC 
AGAGATGAAG TGGCCCAGAT GTACTGCTTA GTAAAAGATT GGCCTCCAAT CAAACCAGAG 
CAAGCCATGG AGCTCCTGGA CTGTAACTAC CCAGACCCCA TGGTTCGGAG CTTTGCTGTC 
CGGTGCTTGG AAAAATACTT AACAGATGAC AAACTTTCTC AGTACCTCAT CCAGCTTGTA 
CAGGTCTTAA AATATGAACA GTATTTGGAT AACCTGCTTG TGAGATTTTT ACTCAAGAAA 
GCACTGACAA ATCAAAGGAT TGGCCATTTT TTCTTTTGGC ATTTAAAATC TGAGATGCAC 
AATAAGACTG TCAGTCAGAG GTTCAGCCTG CTGTTGGAGT CCTACTGCCG TGCCTGTGGG 
ATGTATCTGA AGCACCTGAA CAGACAGGTA GAGGCCATGG AGAAGCTCAT CAATCTAACT 
GACATCCTCA AGCAAGAGAA GAAGGATGAG ACACAGAAGG TACAGATGAA GTTCTTGGTT 
GAACAGATGA GACAGCCAGA TTTCATGGAT GCTTTGCAGG GTTTTCTGTC CCCTCTAAAT 
CCTGCTCATC AACTAGGAAA CCTCAGGCTT GAAGAGTGTC GAATTATGTC CTCTGCAAAA 
AGGCCACTGT GGTTGAATTG GGAGAACCCA GACATCATGT CAGAGCTACT GTTTCAGAAC 
AATGAGATCA TCTTTAAAAA TGGCGATGAC TTACGGCAAG ACATGTTAAC CCTTNNCAGA 
ATCATGGAGA ACATCTGGCA AAACCAAGGC CTTGACCTTC GCATGCTACC TTATGCCTGT 
CTATCCATTG GGGACTGTGT GGGTCTCATC GAGGTGGTGA GAAACTCTCA CACCATCATG 
CAGATTCAGT GCAAAGGAGG CCTGAAGGGG GCACTGCAGT TCAACAGGCA CACGCTGCAT 
CAGTGGCTCA AGGACAAGAA CAAGGGCAAG ATATATGACG CAGCCATTGA CCTGTTCACT 
TGGTCCTGCG CTGGGTACTG CGTGGCAACC TTTATCTTGG GAATTGGAGA CCGGCACAAC 
AGCAACATCA TGGTGAAAGA TGACGGACAG CTGTTTCATA TAGATTTTGG GCACTTTTTG 
GATCACAAGA AGAAAAAATT TGGCTATAAA CGGGAACGCG TGCCGTTTGT TTTGACGCAG 
GATTTCTTAA TAGTGATTAG TAAAGGAGCA CAAGAGTACA CAAAGACCAG AGAGTTTGAG 
AGGTTTCAGG AGATGTGTTA CAAGGCGTAC CTAGCAATTC GGCAGCATGC CAATCTCTTC 
ATCAACCTTT TCTCCATGAT GCTTGGCTCC GGAATGCCAG AACTGCAGTC TTTTGATGAT 
ATTGCATATA TTCGAAAGAC TCTAGCCTTA GACAAAACTG AGCAAGAGGC TCTGGAGTAT 
TTCACAAAGC AAATGAATGA CGCACATCAT GGTGGCTGGA CAACAAAAAT GGACTGGATC 
TTCCACACCA TCAAGCAGCA TGCATTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC685590 ( XM_017588100.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017443589.1 Rattus norvegicus similar to Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PI3-kinase p110 subunit alpha) (PtdIns-3-kinase p110) (PI3K) (LOC685590), mRNA.

Price & Availability↑

CloneID	ORa56111
Clone ID Related Accession (Same CDS sequence)	XM_017602181.1 , XM_017588100.1
Accession Version	XM_017588100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	LOW QUALITY PROTEIN: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases frameshifts :: corrected 2 indels internal stop codons :: corrected 1 genomic stop codons ##RefSeq-Attributes-END##

ATGTGCATGC AGATGTCCAT GGAAAAGTTT CAGGCAGTTG TGAATTGCCC AGTTTTGCAG 
AGGGTCAGAA CAATTCCTCC ACGACCATCT TCGGGTGAAC TGTGGGGCAT CCACTTGATG 
CCCCCACGAA TCCTAGTGGA ATGTTTACTC CCAAATGGAA TGATAGTGAC TTTAGAATGC 
CTCCGTGAGG CCACACTAGT CACCATCAAG CATGAACTGT TCAAAGAGGC CAGGAAATAC 
CCTCTCCATC AGCTTCTGCA AGATGAATCA TCTTACATTT TCGTAAGTGT TACCCAAGAA 
GCAGAAAGGG AAGAATTTTT CGATGAAACA AGACGGCTTT GTGACCTTCG GCTTTTTCAA 
CCCTTTTTAA AAGTAATTGA GCCAGTAGGC AACCGTGAAG AAAAGATCCT CAACCGAGAA 
ATTGGTTTTG TTATTGGCAT GCCAGTGTGT GAATTTGATA TGGTTAAAGA TCTAGAAGTC 
CAAGACTTCC GANAGAACAT TCTGAATGTT TGCAAAGAAG CCGTGGGCCT GCGGGATCTC 
AACTCGCCTC ATAGCAGAGC AATGTATGTC TACCCTCCAA ATGTCGAGTC TTCCCCAGAA 
CTGCCAAAGC ACATCTACAA CAAGTTAGAT AAAGGACAAA TCATAGTGGT GATTTGGGTG 
ATAGTCTCTC CAAACAACGA CAAGCAGAAG TACACTCTGA AGATCAACCA TGACTGCGTG 
CCAGAGCAAG TCATTGCTGA GGCCATCAGG AAGAAGACCC GGAGCATGTT GCTGTCCTCG 
GAGCAGCTGA AACTCTGTGT CTTAGAATAC CAGGGCAAGT ACATTCTCAA AGTGTGTGGC 
TGTGATGAGT ACTTCCTAGA GAAGTACCCT CTGAGTCAGT ACAAGTACAT AAGAAGCTGT 
ATAATGCTGG GGAGGATGCC CAACTTGATG CTGATGGCCA AGGAGAGCCT GCACTCTCAG 
CTGCCGATCG ATAGCTTCAC AATGCCATCC TACTCCAGGC GCATTTCCAC AGCGACACCC 
TATATGAACG GGGAGACTGC TACGAAATCT CTCTGGGTTA TAAATAGCGC GCTCAGAATA 
AAAATTCTGT GTGCAACCTA TGTAAATGTA AATATTCGAG ACATTGATAA GATCTATGTT 
CGAACAGGTA TCTACCATGG AGGAGAATCC TTATGTGACA ATGTGAATAC TCAAAGAGTC 
CCTTGTTCCA ATCCTAGGTG GAATGAATGG CTGAATTATG ATATATACAT TCCTGATCTT 
CCTCGTGCTG CCCGCCTTTG CCTTTCAATC TGCTCTGTTA AAGGCCGAAA GGGTGCTAAG 
GAGGAGCACT GTCCGTTGGC CTGGGGAAAC ATAAACTTGT TTGATTATAC AGACACCCTA 
GTGTCCGGGA AAATGGCTTT GAATCTCTGG CCTGTACCAC ATGGGTTGGA AGATCTGCTG 
AACCCTATTG GTGTTACTGG GTCAAATCCA AATAAAGAAA CTCCATGCTT AGAGTTGGAG 
TTTGATTGGT TCAGCAGTGT GGTGAAGTTT CCAGATATGT CTGTGATCGA AGAGCATGCC 
AATTGGTCTG TGTCCCGAGA AGCCGGATTC AGTTACTCTC ATACAGGACT GAGTAACAGA 
CTAGCCAGAG ACAATGAGTT AAGAGAAAAT GACAAGGAAC AGCTCTGAGC ACTTTGTACC 
CGGGACCCAC TGTCTGAAAT CACTGAACAA GAGAAAGACT TCCTATGGAG CCACAGACAC 
TACTGTGTAA CTATTCCTGA AATCCTACCC AAATTGCTTC TGTCTGTCAA GTGGAATTCC 
AGAGATGAAG TGGCCCAGAT GTACTGCTTA GTAAAAGATT GGCCTCCAAT CAAACCAGAG 
CAAGCCATGG AGCTCCTGGA CTGTAACTAC CCAGACCCCA TGGTTCGGAG CTTTGCTGTC 
CGGTGCTTGG AAAAATACTT AACAGATGAC AAACTTTCTC AGTACCTCAT CCAGCTTGTA 
CAGGTCTTAA AATATGAACA GTATTTGGAT AACCTGCTTG TGAGATTTTT ACTCAAGAAA 
GCACTGACAA ATCAAAGGAT TGGCCATTTT TTCTTTTGGC ATTTAAAATC TGAGATGCAC 
AATAAGACTG TCAGTCAGAG GTTCAGCCTG CTGTTGGAGT CCTACTGCCG TGCCTGTGGG 
ATGTATCTGA AGCACCTGAA CAGACAGGTA GAGGCCATGG AGAAGCTCAT CAATCTAACT 
GACATCCTCA AGCAAGAGAA GAAGGATGAG ACACAGAAGG TACAGATGAA GTTCTTGGTT 
GAACAGATGA GACAGCCAGA TTTCATGGAT GCTTTGCAGG GTTTTCTGTC CCCTCTAAAT 
CCTGCTCATC AACTAGGAAA CCTCAGGCTT GAAGAGTGTC GAATTATGTC CTCTGCAAAA 
AGGCCACTGT GGTTGAATTG GGAGAACCCA GACATCATGT CAGAGCTACT GTTTCAGAAC 
AATGAGATCA TCTTTAAAAA TGGCGATGAC TTACGGCAAG ACATGTTAAC CCTTNNCAGA 
ATCATGGAGA ACATCTGGCA AAACCAAGGC CTTGACCTTC GCATGCTACC TTATGCCTGT 
CTATCCATTG GGGACTGTGT GGGTCTCATC GAGGTGGTGA GAAACTCTCA CACCATCATG 
CAGATTCAGT GCAAAGGAGG CCTGAAGGGG GCACTGCAGT TCAACAGGCA CACGCTGCAT 
CAGTGGCTCA AGGACAAGAA CAAGGGCAAG ATATATGACG CAGCCATTGA CCTGTTCACT 
TGGTCCTGCG CTGGGTACTG CGTGGCAACC TTTATCTTGG GAATTGGAGA CCGGCACAAC 
AGCAACATCA TGGTGAAAGA TGACGGACAG CTGTTTCATA TAGATTTTGG GCACTTTTTG 
GATCACAAGA AGAAAAAATT TGGCTATAAA CGGGAACGCG TGCCGTTTGT TTTGACGCAG 
GATTTCTTAA TAGTGATTAG TAAAGGAGCA CAAGAGTACA CAAAGACCAG AGAGTTTGAG 
AGGTTTCAGG AGATGTGTTA CAAGGCGTAC CTAGCAATTC GGCAGCATGC CAATCTCTTC 
ATCAACCTTT TCTCCATGAT GCTTGGCTCC GGAATGCCAG AACTGCAGTC TTTTGATGAT 
ATTGCATATA TTCGAAAGAC TCTAGCCTTA GACAAAACTG AGCAAGAGGC TCTGGAGTAT 
TTCACAAAGC AAATGAATGA CGCACATCAT GGTGGCTGGA CAACAAAAAT GGACTGGATC 
TTCCACACCA TCAAGCAGCA TGCATTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017443589.1
CDS	1..3273
Translation	MCMQMSMEKFQAVVNCPVLQRVRTIPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLVTIKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFVIGMPVCEFDMVKDLEVQDFRnullNILNVCKEAVGLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLHSQLPIDSFTMPSYSRRISTATPYMNGETATKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGESLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHTGLSNRLARDNELRENDKEQL*ALCTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRSFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFSLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRQPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLnullRIMENIWQNQGLDLRMLPYACLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNRHTLHQWLKDKNKGKIYDAAIDLFTWSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQEYTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFTKQMNDAHHGGWTTKMDWIFHTIKQHALN

Target ORF information:

RefSeq Version	XM_017588100.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus similar to Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PI3-kinase p110 subunit alpha) (PtdIns-3-kinase p110) (PI3K) (LOC685590), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588100.1

ORF Insert Sequence:

ATGTGCATGC AGATGTCCAT GGAAAAGTTT CAGGCAGTTG TGAATTGCCC AGTTTTGCAG 
AGGGTCAGAA CAATTCCTCC ACGACCATCT TCGGGTGAAC TGTGGGGCAT CCACTTGATG 
CCCCCACGAA TCCTAGTGGA ATGTTTACTC CCAAATGGAA TGATAGTGAC TTTAGAATGC 
CTCCGTGAGG CCACACTAGT CACCATCAAG CATGAACTGT TCAAAGAGGC CAGGAAATAC 
CCTCTCCATC AGCTTCTGCA AGATGAATCA TCTTACATTT TCGTAAGTGT TACCCAAGAA 
GCAGAAAGGG AAGAATTTTT CGATGAAACA AGACGGCTTT GTGACCTTCG GCTTTTTCAA 
CCCTTTTTAA AAGTAATTGA GCCAGTAGGC AACCGTGAAG AAAAGATCCT CAACCGAGAA 
ATTGGTTTTG TTATTGGCAT GCCAGTGTGT GAATTTGATA TGGTTAAAGA TCTAGAAGTC 
CAAGACTTCC GANAGAACAT TCTGAATGTT TGCAAAGAAG CCGTGGGCCT GCGGGATCTC 
AACTCGCCTC ATAGCAGAGC AATGTATGTC TACCCTCCAA ATGTCGAGTC TTCCCCAGAA 
CTGCCAAAGC ACATCTACAA CAAGTTAGAT AAAGGACAAA TCATAGTGGT GATTTGGGTG 
ATAGTCTCTC CAAACAACGA CAAGCAGAAG TACACTCTGA AGATCAACCA TGACTGCGTG 
CCAGAGCAAG TCATTGCTGA GGCCATCAGG AAGAAGACCC GGAGCATGTT GCTGTCCTCG 
GAGCAGCTGA AACTCTGTGT CTTAGAATAC CAGGGCAAGT ACATTCTCAA AGTGTGTGGC 
TGTGATGAGT ACTTCCTAGA GAAGTACCCT CTGAGTCAGT ACAAGTACAT AAGAAGCTGT 
ATAATGCTGG GGAGGATGCC CAACTTGATG CTGATGGCCA AGGAGAGCCT GCACTCTCAG 
CTGCCGATCG ATAGCTTCAC AATGCCATCC TACTCCAGGC GCATTTCCAC AGCGACACCC 
TATATGAACG GGGAGACTGC TACGAAATCT CTCTGGGTTA TAAATAGCGC GCTCAGAATA 
AAAATTCTGT GTGCAACCTA TGTAAATGTA AATATTCGAG ACATTGATAA GATCTATGTT 
CGAACAGGTA TCTACCATGG AGGAGAATCC TTATGTGACA ATGTGAATAC TCAAAGAGTC 
CCTTGTTCCA ATCCTAGGTG GAATGAATGG CTGAATTATG ATATATACAT TCCTGATCTT 
CCTCGTGCTG CCCGCCTTTG CCTTTCAATC TGCTCTGTTA AAGGCCGAAA GGGTGCTAAG 
GAGGAGCACT GTCCGTTGGC CTGGGGAAAC ATAAACTTGT TTGATTATAC AGACACCCTA 
GTGTCCGGGA AAATGGCTTT GAATCTCTGG CCTGTACCAC ATGGGTTGGA AGATCTGCTG 
AACCCTATTG GTGTTACTGG GTCAAATCCA AATAAAGAAA CTCCATGCTT AGAGTTGGAG 
TTTGATTGGT TCAGCAGTGT GGTGAAGTTT CCAGATATGT CTGTGATCGA AGAGCATGCC 
AATTGGTCTG TGTCCCGAGA AGCCGGATTC AGTTACTCTC ATACAGGACT GAGTAACAGA 
CTAGCCAGAG ACAATGAGTT AAGAGAAAAT GACAAGGAAC AGCTCTGAGC ACTTTGTACC 
CGGGACCCAC TGTCTGAAAT CACTGAACAA GAGAAAGACT TCCTATGGAG CCACAGACAC 
TACTGTGTAA CTATTCCTGA AATCCTACCC AAATTGCTTC TGTCTGTCAA GTGGAATTCC 
AGAGATGAAG TGGCCCAGAT GTACTGCTTA GTAAAAGATT GGCCTCCAAT CAAACCAGAG 
CAAGCCATGG AGCTCCTGGA CTGTAACTAC CCAGACCCCA TGGTTCGGAG CTTTGCTGTC 
CGGTGCTTGG AAAAATACTT AACAGATGAC AAACTTTCTC AGTACCTCAT CCAGCTTGTA 
CAGGTCTTAA AATATGAACA GTATTTGGAT AACCTGCTTG TGAGATTTTT ACTCAAGAAA 
GCACTGACAA ATCAAAGGAT TGGCCATTTT TTCTTTTGGC ATTTAAAATC TGAGATGCAC 
AATAAGACTG TCAGTCAGAG GTTCAGCCTG CTGTTGGAGT CCTACTGCCG TGCCTGTGGG 
ATGTATCTGA AGCACCTGAA CAGACAGGTA GAGGCCATGG AGAAGCTCAT CAATCTAACT 
GACATCCTCA AGCAAGAGAA GAAGGATGAG ACACAGAAGG TACAGATGAA GTTCTTGGTT 
GAACAGATGA GACAGCCAGA TTTCATGGAT GCTTTGCAGG GTTTTCTGTC CCCTCTAAAT 
CCTGCTCATC AACTAGGAAA CCTCAGGCTT GAAGAGTGTC GAATTATGTC CTCTGCAAAA 
AGGCCACTGT GGTTGAATTG GGAGAACCCA GACATCATGT CAGAGCTACT GTTTCAGAAC 
AATGAGATCA TCTTTAAAAA TGGCGATGAC TTACGGCAAG ACATGTTAAC CCTTNNCAGA 
ATCATGGAGA ACATCTGGCA AAACCAAGGC CTTGACCTTC GCATGCTACC TTATGCCTGT 
CTATCCATTG GGGACTGTGT GGGTCTCATC GAGGTGGTGA GAAACTCTCA CACCATCATG 
CAGATTCAGT GCAAAGGAGG CCTGAAGGGG GCACTGCAGT TCAACAGGCA CACGCTGCAT 
CAGTGGCTCA AGGACAAGAA CAAGGGCAAG ATATATGACG CAGCCATTGA CCTGTTCACT 
TGGTCCTGCG CTGGGTACTG CGTGGCAACC TTTATCTTGG GAATTGGAGA CCGGCACAAC 
AGCAACATCA TGGTGAAAGA TGACGGACAG CTGTTTCATA TAGATTTTGG GCACTTTTTG 
GATCACAAGA AGAAAAAATT TGGCTATAAA CGGGAACGCG TGCCGTTTGT TTTGACGCAG 
GATTTCTTAA TAGTGATTAG TAAAGGAGCA CAAGAGTACA CAAAGACCAG AGAGTTTGAG 
AGGTTTCAGG AGATGTGTTA CAAGGCGTAC CTAGCAATTC GGCAGCATGC CAATCTCTTC 
ATCAACCTTT TCTCCATGAT GCTTGGCTCC GGAATGCCAG AACTGCAGTC TTTTGATGAT 
ATTGCATATA TTCGAAAGAC TCTAGCCTTA GACAAAACTG AGCAAGAGGC TCTGGAGTAT 
TTCACAAAGC AAATGAATGA CGCACATCAT GGTGGCTGGA CAACAAAAAT GGACTGGATC 
TTCCACACCA TCAAGCAGCA TGCATTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC685590 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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