Olah cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Olah
Entrez Gene ID	64669
Full Name	oleoyl-ACP hydrolase
Synonyms	Mch,Thedc1
General protein information	Preferred Names oleoyl-ACP hydrolase Names S-acyl fatty acid synthase thioesterase, medium chain medium-chain S-acyl fatty acid synthetase thio ester hydrolase (MCH) thioesterase II thioesterase domain containing 1 thioesterase domain-containing protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.3
Summary	expressed in mammary gland [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017600660.1	XP_017456149.1	S-acyl fatty acid synthase thioesterase, medium chain isoform X1
NM_022705.1	NP_073196.1	S-acyl fatty acid synthase thioesterase, medium chain
XM_008771887.2	XP_008770109.1	S-acyl fatty acid synthase thioesterase, medium chain isoform X1
XM_008771886.2	XP_008770108.1	S-acyl fatty acid synthase thioesterase, medium chain isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Olah	NP_666033.1
Gallus gallus (chicken)	OLAH	XP_004939320.1
Xenopus tropicalis (tropical clawed frog)	olah	NP_001001207.1
Rattus norvegicus (Norway rat)	Olah	NP_073196.1
Pan troglodytes (chimpanzee)	OLAH	XP_003312512.1
Danio rerio (zebrafish)	olah	NP_001035021.1
Homo sapiens (human)	OLAH	NP_001034791.1

Gene Ontology
Bibliography

Related articles in PubMed

Crystal Structure and Substrate Specificity of Human Thioesterase 2: INSIGHTS INTO THE MOLECULAR BASIS FOR THE MODULATION OF FATTY ACID SYNTHASE.
Ritchie MK, Johnson LC, Clodfelter JE, Pemble CW 4th, Fulp BE, Furdui CM, Kridel SJ, Lowther WT
The Journal of biological chemistry291(7)3520-30(2016 Feb)

Sex difference in the response of melanin-concentrating hormone neurons in the lateral hypothalamic area to glucose, as revealed by the expression of phosphorylated cyclic adenosine 3',5'-monophosphate response element-binding protein.
Mogi K, Funabashi T, Mitsushima D, Hagiwara H, Kimura F
Endocrinology146(8)3325-33(2005 Aug)

Roles of Ser101, Asp236, and His237 in catalysis of thioesterase II and of the C-terminal region of the enzyme in its interaction with fatty acid synthase.
Tai MH, Chirala SS, Wakil SJ
Proceedings of the National Academy of Sciences of the United States of America90(5)1852-6(1993 Mar)

Cloning and sequencing of the medium-chain S-acyl fatty acid synthetase thioester hydrolase cDNA from rat mammary gland.
Naggert J, Williams B, Cashman DP, Smith S
The Biochemical journal243(2)597-601(1987 Apr)

Complete amino acid sequence of the medium-chain S-acyl fatty acid synthetase thio ester hydrolase from rat mammary gland.
Randhawa ZI, Smith S
Biochemistry26(5)1365-73(1987 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Olah gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Olah cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42486	NM_022705.1 Latest version!	Rattus norvegicus oleoyl-ACP hydrolase (Olah), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa38070	XM_008771887.2 Latest version!	Rattus norvegicus oleoyl-ACP hydrolase (Olah), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa38070	XM_017600660.1 Latest version!	Rattus norvegicus oleoyl-ACP hydrolase (Olah), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa38070	XM_008771886.2 Latest version!	Rattus norvegicus oleoyl-ACP hydrolase (Olah), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Olah ( NM_022705.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_073196.1 Rattus norvegicus oleoyl-ACP hydrolase (Olah), mRNA.

Price & Availability↑

CloneID	ORa42486
Clone ID Related Accession (Same CDS sequence)	NM_022705.1
Accession Version	NM_022705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Rattus norvegicus(Norway rat)
Product	S-acyl fatty acid synthase thioesterase, medium chain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M16200.1. ##Evidence-Data-START## Transcript exon combination :: M16200.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGGAGACAG CAGTCAATGC TAAGAGTCCC AGGAATGAAA AGGTTTTGAA CTGTTTGTAT 
CAAAATCCTG ATGCAGTTTT CAAGCTGATC TGCTTCCCTT GGGCAGGAGG CGGCTCCATC 
CATTTTGCCA AGTGGGGCCA AAAGATTAAC GACTCTCTGG AAGTGCATGC TGTAAGACTG 
GCTGGAAGAG AAACCCGACT TGGAGAACCT TTCGCAAATG ACATCTACCA GATAGCTGAT 
GAAATCGTGA CCGCCCTGTT GCCCATCATT CAGGATAAAG CTTTTGCGTT TTTTGGCCAC 
AGTTTTGGAT CCTACATTGC TCTTATTACT GCTCTGCTCC TAAAGGAGAA ATACAAAATG 
GAGCCGCTGC ATATTTTTGT ATCCGGTGCA TCCGCCCCTC ACTCAACATC CCGGCCTCAA 
GTTCCTGATC TTAACGAATT GACAGAAGAA CAAGTCAGAC ATCACCTTCT GGATTTCGGA 
GGCACGCCCA AGCATCTCAT AGAAGACCAG GATGTTCTGA GGATGTTCAT TCCTTTGCTG 
AAGGCAGATG CTGGCGTTGT GAAAAAATTC ATCTTTGACA AGCCCTCCAA AGCTCTTCTC 
TCTCTGGACA TAACGGGCTT CCTTGGATCT GAAGATACAA TAAAGGACAT AGAAGGCTGG 
CAAGACCTAA CCAGTGGGAA GTTTGATGTC CACATGCTGC CAGGCGACCA CTTTTATCTG 
ATGAAGCCCG ACAACGAGAA CTTTATCAAG AACTACATAG CCAAGTGCTT GGAACTCTCG 
TCACTCACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073196.1
CDS	317..1108
Translation	METAVNAKSPRNEKVLNCLYQNPDAVFKLICFPWAGGGSIHFAKWGQKINDSLEVHAVRLAGRETRLGEPFANDIYQIADEIVTALLPIIQDKAFAFFGHSFGSYIALITALLLKEKYKMEPLHIFVSGASAPHSTSRPQVPDLNELTEEQVRHHLLDFGGTPKHLIEDQDVLRMFIPLLKADAGVVKKFIFDKPSKALLSLDITGFLGSEDTIKDIEGWQDLTSGKFDVHMLPGDHFYLMKPDNENFIKNYIAKCLELSSLT

Target ORF information:

RefSeq Version	NM_022705.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus oleoyl-ACP hydrolase (Olah), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022705.1

ORF Insert Sequence:

ATGGAGACAG CAGTCAATGC TAAGAGTCCC AGGAATGAAA AGGTTTTGAA CTGTTTGTAT 
CAAAATCCTG ATGCAGTTTT CAAGCTGATC TGCTTCCCTT GGGCAGGAGG CGGCTCCATC 
CATTTTGCCA AGTGGGGCCA AAAGATTAAC GACTCTCTGG AAGTGCATGC TGTAAGACTG 
GCTGGAAGAG AAACCCGACT TGGAGAACCT TTCGCAAATG ACATCTACCA GATAGCTGAT 
GAAATCGTGA CCGCCCTGTT GCCCATCATT CAGGATAAAG CTTTTGCGTT TTTTGGCCAC 
AGTTTTGGAT CCTACATTGC TCTTATTACT GCTCTGCTCC TAAAGGAGAA ATACAAAATG 
GAGCCGCTGC ATATTTTTGT ATCCGGTGCA TCCGCCCCTC ACTCAACATC CCGGCCTCAA 
GTTCCTGATC TTAACGAATT GACAGAAGAA CAAGTCAGAC ATCACCTTCT GGATTTCGGA 
GGCACGCCCA AGCATCTCAT AGAAGACCAG GATGTTCTGA GGATGTTCAT TCCTTTGCTG 
AAGGCAGATG CTGGCGTTGT GAAAAAATTC ATCTTTGACA AGCCCTCCAA AGCTCTTCTC 
TCTCTGGACA TAACGGGCTT CCTTGGATCT GAAGATACAA TAAAGGACAT AGAAGGCTGG 
CAAGACCTAA CCAGTGGGAA GTTTGATGTC CACATGCTGC CAGGCGACCA CTTTTATCTG 
ATGAAGCCCG ACAACGAGAA CTTTATCAAG AACTACATAG CCAAGTGCTT GGAACTCTCG 
TCACTCACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Olah ( XM_017600660.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456149.1 Rattus norvegicus oleoyl-ACP hydrolase (Olah), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38070
Clone ID Related Accession (Same CDS sequence)	XM_017600660.1 , XM_008771887.2 , XM_008771886.2
Accession Version	XM_017600660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	S-acyl fatty acid synthase thioesterase, medium chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG CAGTCAATGC TAAGAGTCCC AGGAATGAAA AGGTTTTGAA CTGTTTGTAT 
CAAAATCCTG ATGCAGTTTT CAAGCTGATC TGCTTCCCTT GGGCAGGAGG CGGCTCCATC 
CATTTTGCCA AGTGGGGCCA AAAGATTAAC GACTCTCTGG AAGTGCATGC TGTAAGACTG 
GCTGGAAGAG AAACCCGACT TGGAGAACCT TTCGCAAATG ACATCTACCA GATAGCTGAT 
GAAATCGTGA CCGCCCTGTT GCCCATCATT CAGGATAAAG CTTTTGCGTT TTTTGGCCAC 
AGTTTTGGAT CCTACATTGC TCTTATTACT GCTCTGCTCC TAAAGGAGAA ATACAAAATG 
GAGCCACTGC ATATTTTTGT ATCCGGTGCA TCCGCCCCTC ACTCAACATC CCGGCCTCAA 
GTTCCTGATC TTAACGAATT GACAGAAGAA CAAGTCAGAC ATCACCTTCT GGATTTCGGA 
GGCACGCCCA AGCATCTCAT AGAAGACCAG GATGTTCTGA GGATGTTCAT TCCTTTGCTG 
AAGGCAGATG CTGGCGTTGT GAAAAAATTC ATCTTTGACA AGCCCTCCAA AGCTCTTCTC 
TCTCTGGACA TAACGGGCTT CCTTGGATCT GAAGATACAA TAAAGGACAT AGAAGGCTGG 
CAAGACCTAA CCAGTGGGAA GTTTGATGTC CACATGCTGC CAGGTGACCA CTTTTATCTG 
ATGAAGCCCG ACAACGAGAA CTTTATCAAG AACTACATAG CCAAGTGCTT GGAACTCTCG 
TCACTCACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456149.1
CDS	675..1466
Translation	METAVNAKSPRNEKVLNCLYQNPDAVFKLICFPWAGGGSIHFAKWGQKINDSLEVHAVRLAGRETRLGEPFANDIYQIADEIVTALLPIIQDKAFAFFGHSFGSYIALITALLLKEKYKMEPLHIFVSGASAPHSTSRPQVPDLNELTEEQVRHHLLDFGGTPKHLIEDQDVLRMFIPLLKADAGVVKKFIFDKPSKALLSLDITGFLGSEDTIKDIEGWQDLTSGKFDVHMLPGDHFYLMKPDNENFIKNYIAKCLELSSLT

Target ORF information:

RefSeq Version	XM_017600660.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus oleoyl-ACP hydrolase (Olah), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600660.1

ORF Insert Sequence:

ATGGAGACAG CAGTCAATGC TAAGAGTCCC AGGAATGAAA AGGTTTTGAA CTGTTTGTAT 
CAAAATCCTG ATGCAGTTTT CAAGCTGATC TGCTTCCCTT GGGCAGGAGG CGGCTCCATC 
CATTTTGCCA AGTGGGGCCA AAAGATTAAC GACTCTCTGG AAGTGCATGC TGTAAGACTG 
GCTGGAAGAG AAACCCGACT TGGAGAACCT TTCGCAAATG ACATCTACCA GATAGCTGAT 
GAAATCGTGA CCGCCCTGTT GCCCATCATT CAGGATAAAG CTTTTGCGTT TTTTGGCCAC 
AGTTTTGGAT CCTACATTGC TCTTATTACT GCTCTGCTCC TAAAGGAGAA ATACAAAATG 
GAGCCACTGC ATATTTTTGT ATCCGGTGCA TCCGCCCCTC ACTCAACATC CCGGCCTCAA 
GTTCCTGATC TTAACGAATT GACAGAAGAA CAAGTCAGAC ATCACCTTCT GGATTTCGGA 
GGCACGCCCA AGCATCTCAT AGAAGACCAG GATGTTCTGA GGATGTTCAT TCCTTTGCTG 
AAGGCAGATG CTGGCGTTGT GAAAAAATTC ATCTTTGACA AGCCCTCCAA AGCTCTTCTC 
TCTCTGGACA TAACGGGCTT CCTTGGATCT GAAGATACAA TAAAGGACAT AGAAGGCTGG 
CAAGACCTAA CCAGTGGGAA GTTTGATGTC CACATGCTGC CAGGTGACCA CTTTTATCTG 
ATGAAGCCCG ACAACGAGAA CTTTATCAAG AACTACATAG CCAAGTGCTT GGAACTCTCG 
TCACTCACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Olah ( XM_008771887.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770109.1 Rattus norvegicus oleoyl-ACP hydrolase (Olah), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa38070
Clone ID Related Accession (Same CDS sequence)	XM_017600660.1 , XM_008771887.2 , XM_008771886.2
Accession Version	XM_008771887.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	S-acyl fatty acid synthase thioesterase, medium chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG CAGTCAATGC TAAGAGTCCC AGGAATGAAA AGGTTTTGAA CTGTTTGTAT 
CAAAATCCTG ATGCAGTTTT CAAGCTGATC TGCTTCCCTT GGGCAGGAGG CGGCTCCATC 
CATTTTGCCA AGTGGGGCCA AAAGATTAAC GACTCTCTGG AAGTGCATGC TGTAAGACTG 
GCTGGAAGAG AAACCCGACT TGGAGAACCT TTCGCAAATG ACATCTACCA GATAGCTGAT 
GAAATCGTGA CCGCCCTGTT GCCCATCATT CAGGATAAAG CTTTTGCGTT TTTTGGCCAC 
AGTTTTGGAT CCTACATTGC TCTTATTACT GCTCTGCTCC TAAAGGAGAA ATACAAAATG 
GAGCCACTGC ATATTTTTGT ATCCGGTGCA TCCGCCCCTC ACTCAACATC CCGGCCTCAA 
GTTCCTGATC TTAACGAATT GACAGAAGAA CAAGTCAGAC ATCACCTTCT GGATTTCGGA 
GGCACGCCCA AGCATCTCAT AGAAGACCAG GATGTTCTGA GGATGTTCAT TCCTTTGCTG 
AAGGCAGATG CTGGCGTTGT GAAAAAATTC ATCTTTGACA AGCCCTCCAA AGCTCTTCTC 
TCTCTGGACA TAACGGGCTT CCTTGGATCT GAAGATACAA TAAAGGACAT AGAAGGCTGG 
CAAGACCTAA CCAGTGGGAA GTTTGATGTC CACATGCTGC CAGGTGACCA CTTTTATCTG 
ATGAAGCCCG ACAACGAGAA CTTTATCAAG AACTACATAG CCAAGTGCTT GGAACTCTCG 
TCACTCACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770109.1
CDS	560..1351
Translation	METAVNAKSPRNEKVLNCLYQNPDAVFKLICFPWAGGGSIHFAKWGQKINDSLEVHAVRLAGRETRLGEPFANDIYQIADEIVTALLPIIQDKAFAFFGHSFGSYIALITALLLKEKYKMEPLHIFVSGASAPHSTSRPQVPDLNELTEEQVRHHLLDFGGTPKHLIEDQDVLRMFIPLLKADAGVVKKFIFDKPSKALLSLDITGFLGSEDTIKDIEGWQDLTSGKFDVHMLPGDHFYLMKPDNENFIKNYIAKCLELSSLT

Target ORF information:

RefSeq Version	XM_008771887.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus oleoyl-ACP hydrolase (Olah), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771887.2

ORF Insert Sequence:

ATGGAGACAG CAGTCAATGC TAAGAGTCCC AGGAATGAAA AGGTTTTGAA CTGTTTGTAT 
CAAAATCCTG ATGCAGTTTT CAAGCTGATC TGCTTCCCTT GGGCAGGAGG CGGCTCCATC 
CATTTTGCCA AGTGGGGCCA AAAGATTAAC GACTCTCTGG AAGTGCATGC TGTAAGACTG 
GCTGGAAGAG AAACCCGACT TGGAGAACCT TTCGCAAATG ACATCTACCA GATAGCTGAT 
GAAATCGTGA CCGCCCTGTT GCCCATCATT CAGGATAAAG CTTTTGCGTT TTTTGGCCAC 
AGTTTTGGAT CCTACATTGC TCTTATTACT GCTCTGCTCC TAAAGGAGAA ATACAAAATG 
GAGCCACTGC ATATTTTTGT ATCCGGTGCA TCCGCCCCTC ACTCAACATC CCGGCCTCAA 
GTTCCTGATC TTAACGAATT GACAGAAGAA CAAGTCAGAC ATCACCTTCT GGATTTCGGA 
GGCACGCCCA AGCATCTCAT AGAAGACCAG GATGTTCTGA GGATGTTCAT TCCTTTGCTG 
AAGGCAGATG CTGGCGTTGT GAAAAAATTC ATCTTTGACA AGCCCTCCAA AGCTCTTCTC 
TCTCTGGACA TAACGGGCTT CCTTGGATCT GAAGATACAA TAAAGGACAT AGAAGGCTGG 
CAAGACCTAA CCAGTGGGAA GTTTGATGTC CACATGCTGC CAGGTGACCA CTTTTATCTG 
ATGAAGCCCG ACAACGAGAA CTTTATCAAG AACTACATAG CCAAGTGCTT GGAACTCTCG 
TCACTCACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Olah ( XM_008771886.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770108.1 Rattus norvegicus oleoyl-ACP hydrolase (Olah), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38070
Clone ID Related Accession (Same CDS sequence)	XM_017600660.1 , XM_008771887.2 , XM_008771886.2
Accession Version	XM_008771886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	S-acyl fatty acid synthase thioesterase, medium chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG CAGTCAATGC TAAGAGTCCC AGGAATGAAA AGGTTTTGAA CTGTTTGTAT 
CAAAATCCTG ATGCAGTTTT CAAGCTGATC TGCTTCCCTT GGGCAGGAGG CGGCTCCATC 
CATTTTGCCA AGTGGGGCCA AAAGATTAAC GACTCTCTGG AAGTGCATGC TGTAAGACTG 
GCTGGAAGAG AAACCCGACT TGGAGAACCT TTCGCAAATG ACATCTACCA GATAGCTGAT 
GAAATCGTGA CCGCCCTGTT GCCCATCATT CAGGATAAAG CTTTTGCGTT TTTTGGCCAC 
AGTTTTGGAT CCTACATTGC TCTTATTACT GCTCTGCTCC TAAAGGAGAA ATACAAAATG 
GAGCCACTGC ATATTTTTGT ATCCGGTGCA TCCGCCCCTC ACTCAACATC CCGGCCTCAA 
GTTCCTGATC TTAACGAATT GACAGAAGAA CAAGTCAGAC ATCACCTTCT GGATTTCGGA 
GGCACGCCCA AGCATCTCAT AGAAGACCAG GATGTTCTGA GGATGTTCAT TCCTTTGCTG 
AAGGCAGATG CTGGCGTTGT GAAAAAATTC ATCTTTGACA AGCCCTCCAA AGCTCTTCTC 
TCTCTGGACA TAACGGGCTT CCTTGGATCT GAAGATACAA TAAAGGACAT AGAAGGCTGG 
CAAGACCTAA CCAGTGGGAA GTTTGATGTC CACATGCTGC CAGGTGACCA CTTTTATCTG 
ATGAAGCCCG ACAACGAGAA CTTTATCAAG AACTACATAG CCAAGTGCTT GGAACTCTCG 
TCACTCACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770108.1
CDS	615..1406
Translation	METAVNAKSPRNEKVLNCLYQNPDAVFKLICFPWAGGGSIHFAKWGQKINDSLEVHAVRLAGRETRLGEPFANDIYQIADEIVTALLPIIQDKAFAFFGHSFGSYIALITALLLKEKYKMEPLHIFVSGASAPHSTSRPQVPDLNELTEEQVRHHLLDFGGTPKHLIEDQDVLRMFIPLLKADAGVVKKFIFDKPSKALLSLDITGFLGSEDTIKDIEGWQDLTSGKFDVHMLPGDHFYLMKPDNENFIKNYIAKCLELSSLT

Target ORF information:

RefSeq Version	XM_008771886.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus oleoyl-ACP hydrolase (Olah), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771886.2

ORF Insert Sequence:

ATGGAGACAG CAGTCAATGC TAAGAGTCCC AGGAATGAAA AGGTTTTGAA CTGTTTGTAT 
CAAAATCCTG ATGCAGTTTT CAAGCTGATC TGCTTCCCTT GGGCAGGAGG CGGCTCCATC 
CATTTTGCCA AGTGGGGCCA AAAGATTAAC GACTCTCTGG AAGTGCATGC TGTAAGACTG 
GCTGGAAGAG AAACCCGACT TGGAGAACCT TTCGCAAATG ACATCTACCA GATAGCTGAT 
GAAATCGTGA CCGCCCTGTT GCCCATCATT CAGGATAAAG CTTTTGCGTT TTTTGGCCAC 
AGTTTTGGAT CCTACATTGC TCTTATTACT GCTCTGCTCC TAAAGGAGAA ATACAAAATG 
GAGCCACTGC ATATTTTTGT ATCCGGTGCA TCCGCCCCTC ACTCAACATC CCGGCCTCAA 
GTTCCTGATC TTAACGAATT GACAGAAGAA CAAGTCAGAC ATCACCTTCT GGATTTCGGA 
GGCACGCCCA AGCATCTCAT AGAAGACCAG GATGTTCTGA GGATGTTCAT TCCTTTGCTG 
AAGGCAGATG CTGGCGTTGT GAAAAAATTC ATCTTTGACA AGCCCTCCAA AGCTCTTCTC 
TCTCTGGACA TAACGGGCTT CCTTGGATCT GAAGATACAA TAAAGGACAT AGAAGGCTGG 
CAAGACCTAA CCAGTGGGAA GTTTGATGTC CACATGCTGC CAGGTGACCA CTTTTATCTG 
ATGAAGCCCG ACAACGAGAA CTTTATCAAG AACTACATAG CCAAGTGCTT GGAACTCTCG 
TCACTCACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal Structure and Substrate Specificity of Human Thioesterase 2: INSIGHTS INTO THE MOLECULAR BASIS FOR THE MODULATION OF FATTY ACID SYNTHASE.
The Journal of biological chemistry291(7)3520-30(2016 Feb)
Ritchie MK,Johnson LC,Clodfelter JE,Pemble CW 4th,Fulp BE,Furdui CM,Kridel SJ,Lowther WT

Sex difference in the response of melanin-concentrating hormone neurons in the lateral hypothalamic area to glucose, as revealed by the expression of phosphorylated cyclic adenosine 3',5'-monophosphate response element-binding protein.
Endocrinology146(8)3325-33(2005 Aug)
Mogi K,Funabashi T,Mitsushima D,Hagiwara H,Kimura F

Roles of Ser101, Asp236, and His237 in catalysis of thioesterase II and of the C-terminal region of the enzyme in its interaction with fatty acid synthase.
Proceedings of the National Academy of Sciences of the United States of America90(5)1852-6(1993 Mar)
Tai MH,Chirala SS,Wakil SJ

Cloning and sequencing of the medium-chain S-acyl fatty acid synthetase thioester hydrolase cDNA from rat mammary gland.
The Biochemical journal243(2)597-601(1987 Apr)
Naggert J,Williams B,Cashman DP,Smith S

Complete amino acid sequence of the medium-chain S-acyl fatty acid synthetase thio ester hydrolase from rat mammary gland.
Biochemistry26(5)1365-73(1987 Mar)
Randhawa ZI,Smith S

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