Dis3l2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dis3l2
Entrez Gene ID	367307
Full Name	DIS3-like 3'-5' exoribonuclease 2
Synonyms	RGD1560168
General protein information	Preferred Names DIS3-like 3'-5' exoribonuclease 2 Names DIS3-like exonuclease 2 DIS3 mitotic control homolog-like 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q35

mRNA and Protein(s)

mRNA	Protein	Name
NM_001109007.2	NP_001102477.2	DIS3-like exonuclease 2 isoform 1
NM_001172159.1	NP_001165630.1	DIS3-like exonuclease 2 isoform 2

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0754100	NP_001048152.1
Homo sapiens (human)	DIS3L2	NP_689596.4
Mus musculus (house mouse)	Dis3l2	NP_705758.1
Danio rerio (zebrafish)	LOC566106	XP_005163305.1
Pan troglodytes (chimpanzee)	DIS3L2	XP_003309564.1
Canis lupus familiaris (dog)	DIS3L2	XP_852722.1
Bos taurus (cattle)	DIS3L2	NP_001179435.1
Arabidopsis thaliana (thale cress)	AT1G77680	NP_177891.1
Rattus norvegicus (Norway rat)	Dis3l2	NP_001102477.2
Gallus gallus (chicken)	DIS3L2	XP_422741.4
Caenorhabditis elegans (roundworm)	F48E8.6	NP_498160.2
Schizosaccharomyces pombe (fission yeast)	SPAC2C4.07c	NP_594510.1
Xenopus tropicalis (tropical clawed frog)	dis3l2	NP_001072804.1

Gene Ontology
Bibliography

Related articles in PubMed

Mechanism of Dis3l2 substrate recognition in the Lin28-let-7 pathway.
Faehnle CR, Walleshauser J, Joshua-Tor L
Nature514(7521)252-256(2014 Oct)

Mammalian DIS3L2 exoribonuclease targets the uridylated precursors of let-7 miRNAs.
Ustianenko D, Hrossova D, Potesil D, Chalupnikova K, Hrazdilova K, Pachernik J, Cetkovska K, Uldrijan S, Zdrahal Z, Vanacova S
RNA (New York, N.Y.)19(12)1632-8(2013 Dec)

Exonuclease hDIS3L2 specifies an exosome-independent 3'-5' degradation pathway of human cytoplasmic mRNA.
Lubas M, Damgaard CK, Tomecki R, Cysewski D, Jensen TH, Dziembowski A
The EMBO journal32(13)1855-68(2013 Jul)

A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.
Chang HM, Triboulet R, Thornton JE, Gregory RI
Nature497(7448)244-8(2013 May)

Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility.
Astuti D, Morris MR, Cooper WN, Staals RH, Wake NC, Fews GA, Gill H, Gentle D, Shuib S, Ricketts CJ, Cole T, van Essen AJ, van Lingen RA, Neri G, Opitz JM, Rump P, Stolte-Dijkstra I, Müller F, Pruijn GJ, Latif F, Maher ER
Nature genetics44(3)277-84(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dis3l2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dis3l2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13856	NM_001172159.1 Latest version!	Rattus norvegicus DIS3-like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa13870	NM_001109007.2 Latest version!	Rattus norvegicus DIS3-like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dis3l2 ( NM_001172159.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001165630.1 Rattus norvegicus DIS3-like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa13856
Clone ID Related Accession (Same CDS sequence)	NM_001172159.1
Accession Version	NM_001172159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527436800000
Organism	Rattus norvegicus(Norway rat)
Product	DIS3-like exonuclease 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CH474004.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCATC CTGACTACAA GCTGAACCTT CAGTCTCCAG GGACTCCCAG AGGTGTGTCC 
TCTGTGGTTG GCCCGAATGC TGTTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAAG 
TCCATGCGAG GGAAGAAAAA GAGCATATTT GAAACCTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAAAGGAAC GCTTATCCAG GGTGTATTGA GGATCAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC TCCGGATGGT GATCGGGACA TTTTTATTGA TGGAGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGACCTT GTGGTTGTAA AACTGCTTCC TGAGGACCAG 
TGGAAGGGAG TTAAACCAGA GAGCAACGAC AAAGAAACAG AAGCTACTAA TGAAACTGAC 
ATCTCTGAGG AGTCCTGTGG ACACCACCTT CTGCAGAAGT CCCCAAAAGG CTGGAATGGT 
CCTGATGTTA TTATAGAGGC TCAGTTTGAT GACAGCGACT CAGAAGATAG ACATGGCAAC 
ACCAGTGGGC TGGTTGATGG TATGAAGAAA TTGTCAGTCT GTACTCCTGA CAAAGGAAAA 
GAAGATTCTA GTGCTCCAGT TATGAAAGAC GAGAACACCC CCAAGTCACA GGACACAAGA 
GCTTTATCAG AGAAGTCACT CCAGAAATCA GCAAAGGTGG TTTACATCTT GGAGAAAAAG 
CATTCCCGAG CAGCAACCGG CATCCTGAAA CTCTTGGCTG ACAAGAACAG TGACCTGTTT 
AAGAAATATG CCCTGTTTTC TCCTTCAGAC CACCGAGTAC CTAGAATTTA TGTACCACTC 
AAGGACTGTC CCCAGGACTT CATGACCCGA CCCAAAGACT ATGCCAACAC GCTGTTCATC 
TGCCGCATCA TAGACTGGAA GGAGGACTGT AATTTTGCCC TGGGGCAACT GGCTAAGAGT 
CTTGGGCAAG CTGGTGAAAT TGAACCTGAA ACAGAAGGGA TCCTGACAGA ATATGGTGTG 
GACTTCTCTG ATTTCTCTTC AGAAGTTCTC GAATGTCTCC CTCAAAGCCT GCCATGGACA 
ATTCCACCTG AGGAGGTGGG CAAGAGAAGA GACCTAAGAA AAGACTGTAT CTTCACCATT 
GACCCATCAA CTGCTCGAGA CCTTGATGAC GCCCTCTCCT GCAGGCAGCT CACTGACGGC 
ACCTTCGAAG TGGGCGTCCA CATAGCCGAT GTGAGTTACT TCGTTCCTGA GGGATCCTCT 
TTGGATAAGG TAGCTGCTGA GAGAGCCACA AGTGTCTACT TGGTCCAGAA GGTGGTCCCC 
ATGCTTCCCA GGCTTCTGTG TGAGGAACTC TGCAGCCTCA ACCCCATGAC TGACAAGCTG 
ACCTTCTCTG TGATCTGGAA GCTGACTCCT GAAGGCAAGA TCCTTGAGGA GTGGTTTGGC 
CGCACTATCA TCCGTTCTTG CACCAAATTG AGCTACGACC ATGCGCAGAG CATGATCGAA 
AATCCGACTG AGAAGATCCC CAAGGAAGAG CTTCCCCCTA TTTCTCCAGA GCACAGCATC 
GAGGAGGTGC ACCAGGCAGT CCTGAACCTG CACAGCATTG CAAAGCAACT CCGCCGCCAG 
CGCTTTGTAG ATGGTGCACT CCGTTTAGAT CAGCTGAAGC TTGCTTTCAC TCTGGACCAT 
GAGACTGGAT TGCCTCAAGG ATGTCATATC TATGAGTACC GAGACAGCAA CAAGCTTGTA 
GAGGAGTTCA TGCTCCTGGC CAACATGGCA GTGGCCCACA AGATCTTCCG CACCTTCCCT 
GAGCAGGCCC TGCTGCGCCG GCATCCCCCA CCGCAGACGA AGATGCTCAG TGACCTGGTG 
GAGTTCTGTG ACCAGATGGG GCTGCCCATG GATGTCAGCT CTGCAGGGGC CCTAAATAAA 
AGCCTGACCA ACACATTTGG AGATGACAAG TACTCTCTGG CCCGGAAAGA GGTGCTCACC 
AACATGTACT CCCGGCCCAT GCAGATGGCA CTGTACTTCT GCTCTGGGAT GCTGCAGGAC 
CAGGCGCAGT TCCGGCATTA TGCTCTCAAC GTTCCCCTCT ACACACACTT CACCTCTCCC 
ATCCGCCGCT TTGCTGACGT CATAGTGCAC CGCCTCCTGG CTGCTGCTCT GGGCTACAGT 
GAGCAGCCAG ATGTGGAGCC TGATGCCCTA CAGAAGCAAG CCGACCACTG CAATGACCGC 
CGCATGGCCT CTAAGCGTGT GCAGGAGCTC AGCATTGGCC TCTTCTTCTC AATTCTAGTA 
AAAGAGAGCG GCCCCCTGGA GTCTGAAGCC ATGGTGATGG GTGTCCTGAA CCAAGCTTTC 
GATGTGCTGG TACTGCGCTT TGGGGTACAA AAACGCATCT ACTGCAACGC ACTGGCCTTG 
AGGTCCTACC ACTTCCAGAA GGTGGGGAAG AAGCCAGAGC TCACTCTTGT TTGGGAGCCA 
GATGACCTTG AAGAGGAGCC AATACAGCAG GTCATCACCA TCTTCAGCCT GGTGGATGTG 
GTCCTGCAGG CAGAGGCCAC AGCCCTCAAG TACAGTGCTA TCCTGAAGCG ACCAGGCCCG 
GAGCATTCCC TGGGTCTGGA GAAGGAGTCT GATGAGGAGC CTGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165630.1
CDS	150..2780
Translation	MNHPDYKLNLQSPGTPRGVSSVVGPNAVGASPGDKKSKNKSMRGKKKSIFETYMSKEDVSEGLKKGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEDQWKGVKPESNDKETEATNETDISEESCGHHLLQKSPKGWNGPDVIIEAQFDDSDSEDRHGNTSGLVDGMKKLSVCTPDKGKEDSSAPVMKDENTPKSQDTRALSEKSLQKSAKVVYILEKKHSRAATGILKLLADKNSDLFKKYALFSPSDHRVPRIYVPLKDCPQDFMTRPKDYANTLFICRIIDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQSLPWTIPPEEVGKRRDLRKDCIFTIDPSTARDLDDALSCRQLTDGTFEVGVHIADVSYFVPEGSSLDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMTDKLTFSVIWKLTPEGKILEEWFGRTIIRSCTKLSYDHAQSMIENPTEKIPKEELPPISPEHSIEEVHQAVLNLHSIAKQLRRQRFVDGALRLDQLKLAFTLDHETGLPQGCHIYEYRDSNKLVEEFMLLANMAVAHKIFRTFPEQALLRRHPPPQTKMLSDLVEFCDQMGLPMDVSSAGALNKSLTNTFGDDKYSLARKEVLTNMYSRPMQMALYFCSGMLQDQAQFRHYALNVPLYTHFTSPIRRFADVIVHRLLAAALGYSEQPDVEPDALQKQADHCNDRRMASKRVQELSIGLFFSILVKESGPLESEAMVMGVLNQAFDVLVLRFGVQKRIYCNALALRSYHFQKVGKKPELTLVWEPDDLEEEPIQQVITIFSLVDVVLQAEATALKYSAILKRPGPEHSLGLEKESDEEPED

Target ORF information:

RefSeq Version	NM_001172159.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DIS3-like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172159.1

ORF Insert Sequence:

ATGAACCATC CTGACTACAA GCTGAACCTT CAGTCTCCAG GGACTCCCAG AGGTGTGTCC 
TCTGTGGTTG GCCCGAATGC TGTTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAAG 
TCCATGCGAG GGAAGAAAAA GAGCATATTT GAAACCTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAAAGGAAC GCTTATCCAG GGTGTATTGA GGATCAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC TCCGGATGGT GATCGGGACA TTTTTATTGA TGGAGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGACCTT GTGGTTGTAA AACTGCTTCC TGAGGACCAG 
TGGAAGGGAG TTAAACCAGA GAGCAACGAC AAAGAAACAG AAGCTACTAA TGAAACTGAC 
ATCTCTGAGG AGTCCTGTGG ACACCACCTT CTGCAGAAGT CCCCAAAAGG CTGGAATGGT 
CCTGATGTTA TTATAGAGGC TCAGTTTGAT GACAGCGACT CAGAAGATAG ACATGGCAAC 
ACCAGTGGGC TGGTTGATGG TATGAAGAAA TTGTCAGTCT GTACTCCTGA CAAAGGAAAA 
GAAGATTCTA GTGCTCCAGT TATGAAAGAC GAGAACACCC CCAAGTCACA GGACACAAGA 
GCTTTATCAG AGAAGTCACT CCAGAAATCA GCAAAGGTGG TTTACATCTT GGAGAAAAAG 
CATTCCCGAG CAGCAACCGG CATCCTGAAA CTCTTGGCTG ACAAGAACAG TGACCTGTTT 
AAGAAATATG CCCTGTTTTC TCCTTCAGAC CACCGAGTAC CTAGAATTTA TGTACCACTC 
AAGGACTGTC CCCAGGACTT CATGACCCGA CCCAAAGACT ATGCCAACAC GCTGTTCATC 
TGCCGCATCA TAGACTGGAA GGAGGACTGT AATTTTGCCC TGGGGCAACT GGCTAAGAGT 
CTTGGGCAAG CTGGTGAAAT TGAACCTGAA ACAGAAGGGA TCCTGACAGA ATATGGTGTG 
GACTTCTCTG ATTTCTCTTC AGAAGTTCTC GAATGTCTCC CTCAAAGCCT GCCATGGACA 
ATTCCACCTG AGGAGGTGGG CAAGAGAAGA GACCTAAGAA AAGACTGTAT CTTCACCATT 
GACCCATCAA CTGCTCGAGA CCTTGATGAC GCCCTCTCCT GCAGGCAGCT CACTGACGGC 
ACCTTCGAAG TGGGCGTCCA CATAGCCGAT GTGAGTTACT TCGTTCCTGA GGGATCCTCT 
TTGGATAAGG TAGCTGCTGA GAGAGCCACA AGTGTCTACT TGGTCCAGAA GGTGGTCCCC 
ATGCTTCCCA GGCTTCTGTG TGAGGAACTC TGCAGCCTCA ACCCCATGAC TGACAAGCTG 
ACCTTCTCTG TGATCTGGAA GCTGACTCCT GAAGGCAAGA TCCTTGAGGA GTGGTTTGGC 
CGCACTATCA TCCGTTCTTG CACCAAATTG AGCTACGACC ATGCGCAGAG CATGATCGAA 
AATCCGACTG AGAAGATCCC CAAGGAAGAG CTTCCCCCTA TTTCTCCAGA GCACAGCATC 
GAGGAGGTGC ACCAGGCAGT CCTGAACCTG CACAGCATTG CAAAGCAACT CCGCCGCCAG 
CGCTTTGTAG ATGGTGCACT CCGTTTAGAT CAGCTGAAGC TTGCTTTCAC TCTGGACCAT 
GAGACTGGAT TGCCTCAAGG ATGTCATATC TATGAGTACC GAGACAGCAA CAAGCTTGTA 
GAGGAGTTCA TGCTCCTGGC CAACATGGCA GTGGCCCACA AGATCTTCCG CACCTTCCCT 
GAGCAGGCCC TGCTGCGCCG GCATCCCCCA CCGCAGACGA AGATGCTCAG TGACCTGGTG 
GAGTTCTGTG ACCAGATGGG GCTGCCCATG GATGTCAGCT CTGCAGGGGC CCTAAATAAA 
AGCCTGACCA ACACATTTGG AGATGACAAG TACTCTCTGG CCCGGAAAGA GGTGCTCACC 
AACATGTACT CCCGGCCCAT GCAGATGGCA CTGTACTTCT GCTCTGGGAT GCTGCAGGAC 
CAGGCGCAGT TCCGGCATTA TGCTCTCAAC GTTCCCCTCT ACACACACTT CACCTCTCCC 
ATCCGCCGCT TTGCTGACGT CATAGTGCAC CGCCTCCTGG CTGCTGCTCT GGGCTACAGT 
GAGCAGCCAG ATGTGGAGCC TGATGCCCTA CAGAAGCAAG CCGACCACTG CAATGACCGC 
CGCATGGCCT CTAAGCGTGT GCAGGAGCTC AGCATTGGCC TCTTCTTCTC AATTCTAGTA 
AAAGAGAGCG GCCCCCTGGA GTCTGAAGCC ATGGTGATGG GTGTCCTGAA CCAAGCTTTC 
GATGTGCTGG TACTGCGCTT TGGGGTACAA AAACGCATCT ACTGCAACGC ACTGGCCTTG 
AGGTCCTACC ACTTCCAGAA GGTGGGGAAG AAGCCAGAGC TCACTCTTGT TTGGGAGCCA 
GATGACCTTG AAGAGGAGCC AATACAGCAG GTCATCACCA TCTTCAGCCT GGTGGATGTG 
GTCCTGCAGG CAGAGGCCAC AGCCCTCAAG TACAGTGCTA TCCTGAAGCG ACCAGGCCCG 
GAGCATTCCC TGGGTCTGGA GAAGGAGTCT GATGAGGAGC CTGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dis3l2 ( NM_001109007.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102477.2 Rattus norvegicus DIS3-like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa13870
Clone ID Related Accession (Same CDS sequence)	NM_001109007.2
Accession Version	NM_001109007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527436800000
Organism	Rattus norvegicus(Norway rat)
Product	DIS3-like exonuclease 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CH474004.1. On Feb 5, 2010 this sequence version replaced NM_001109007.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCATC CTGACTACAA GCTGAACCTT CAGTCTCCAG GGACTCCCAG AGGTGTGTCC 
TCTGTGGTTG GCCCGAATGC TGTTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAAG 
TCCATGCGAG GGAAGAAAAA GAGCATATTT GAAACCTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAAAGGAAC GCTTATCCAG GGTGTATTGA GGATCAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC TCCGGATGGT GATCGGGACA TTTTTATTGA TGGAGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGACCTT GTGGTTGTAA AACTGCTTCC TGAGGACCAG 
TGGAAGCCAA GGATAACCTT GTCTCTGCCT GGAGTGCTGG GATTACAGGG AGTTAAACCA 
GAGAGCAACG ACAAAGAAAC AGAAGCTACT AATGAAACTG ACATCTCTGA GGAGTCCTGT 
GGACACCACC TTCTGCAGAA GTCCCCAAAA GGCTGGAATG GTCCTGATGT TATTATAGAG 
GCTCAGTTTG ATGACAGCGA CTCAGAAGAT AGACATGGCA ACACCAGTGG GCTGGTTGAT 
GGTATGAAGA AATTGTCAGT CTGTACTCCT GACAAAGGAA AAGAAGATTC TAGTGCTCCA 
GTTATGAAAG ACGAGAACAC CCCCAAGTCA CAGGACACAA GAGCTTTATC AGAGAAGTCA 
CTCCAGAAAT CAGCAAAGGT GGTTTACATC TTGGAGAAAA AGCATTCCCG AGCAGCAACC 
GGCATCCTGA AACTCTTGGC TGACAAGAAC AGTGACCTGT TTAAGAAATA TGCCCTGTTT 
TCTCCTTCAG ACCACCGAGT ACCTAGAATT TATGTACCAC TCAAGGACTG TCCCCAGGAC 
TTCATGACCC GACCCAAAGA CTATGCCAAC ACGCTGTTCA TCTGCCGCAT CATAGACTGG 
AAGGAGGACT GTAATTTTGC CCTGGGGCAA CTGGCTAAGA GTCTTGGGCA AGCTGGTGAA 
ATTGAACCTG AAACAGAAGG GATCCTGACA GAATATGGTG TGGACTTCTC TGATTTCTCT 
TCAGAAGTTC TCGAATGTCT CCCTCAAAGC CTGCCATGGA CAATTCCACC TGAGGAGGTG 
GGCAAGAGAA GAGACCTAAG AAAAGACTGT ATCTTCACCA TTGACCCATC AACTGCTCGA 
GACCTTGATG ACGCCCTCTC CTGCAGGCAG CTCACTGACG GCACCTTCGA AGTGGGCGTC 
CACATAGCCG ATGTGAGTTA CTTCGTTCCT GAGGGATCCT CTTTGGATAA GGTAGCTGCT 
GAGAGAGCCA CAAGTGTCTA CTTGGTCCAG AAGGTGGTCC CCATGCTTCC CAGGCTTCTG 
TGTGAGGAAC TCTGCAGCCT CAACCCCATG ACTGACAAGC TGACCTTCTC TGTGATCTGG 
AAGCTGACTC CTGAAGGCAA GATCCTTGAG GAGTGGTTTG GCCGCACTAT CATCCGTTCT 
TGCACCAAAT TGAGCTACGA CCATGCGCAG AGCATGATCG AAAATCCGAC TGAGAAGATC 
CCCAAGGAAG AGCTTCCCCC TATTTCTCCA GAGCACAGCA TCGAGGAGGT GCACCAGGCA 
GTCCTGAACC TGCACAGCAT TGCAAAGCAA CTCCGCCGCC AGCGCTTTGT AGATGGTGCA 
CTCCGTTTAG ATCAGCTGAA GCTTGCTTTC ACTCTGGACC ATGAGACTGG ATTGCCTCAA 
GGATGTCATA TCTATGAGTA CCGAGACAGC AACAAGCTTG TAGAGGAGTT CATGCTCCTG 
GCCAACATGG CAGTGGCCCA CAAGATCTTC CGCACCTTCC CTGAGCAGGC CCTGCTGCGC 
CGGCATCCCC CACCGCAGAC GAAGATGCTC AGTGACCTGG TGGAGTTCTG TGACCAGATG 
GGGCTGCCCA TGGATGTCAG CTCTGCAGGG GCCCTAAATA AAAGCCTGAC CAACACATTT 
GGAGATGACA AGTACTCTCT GGCCCGGAAA GAGGTGCTCA CCAACATGTA CTCCCGGCCC 
ATGCAGATGG CACTGTACTT CTGCTCTGGG ATGCTGCAGG ACCAGGCGCA GTTCCGGCAT 
TATGCTCTCA ACGTTCCCCT CTACACACAC TTCACCTCTC CCATCCGCCG CTTTGCTGAC 
GTCATAGTGC ACCGCCTCCT GGCTGCTGCT CTGGGCTACA GTGAGCAGCC AGATGTGGAG 
CCTGATGCCC TACAGAAGCA AGCCGACCAC TGCAATGACC GCCGCATGGC CTCTAAGCGT 
GTGCAGGAGC TCAGCATTGG CCTCTTCTTC TCAATTCTAG TAAAAGAGAG CGGCCCCCTG 
GAGTCTGAAG CCATGGTGAT GGGTGTCCTG AACCAAGCTT TCGATGTGCT GGTACTGCGC 
TTTGGGGTAC AAAAACGCAT CTACTGCAAC GCACTGGCCT TGAGGTCCTA CCACTTCCAG 
AAGGTGGGGA AGAAGCCAGA GCTCACTCTT GTTTGGGAGC CAGATGACCT TGAAGAGGAG 
CCAATACAGC AGGTCATCAC CATCTTCAGC CTGGTGGATG TGGTCCTGCA GGCAGAGGCC 
ACAGCCCTCA AGTACAGTGC TATCCTGAAG CGACCAGGCC CGGAGCATTC CCTGGGTCTG 
GAGAAGGAGT CTGATGAGGA GCCTGAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102477.2
CDS	150..2822
Translation	MNHPDYKLNLQSPGTPRGVSSVVGPNAVGASPGDKKSKNKSMRGKKKSIFETYMSKEDVSEGLKKGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEDQWKPRITLSLPGVLGLQGVKPESNDKETEATNETDISEESCGHHLLQKSPKGWNGPDVIIEAQFDDSDSEDRHGNTSGLVDGMKKLSVCTPDKGKEDSSAPVMKDENTPKSQDTRALSEKSLQKSAKVVYILEKKHSRAATGILKLLADKNSDLFKKYALFSPSDHRVPRIYVPLKDCPQDFMTRPKDYANTLFICRIIDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQSLPWTIPPEEVGKRRDLRKDCIFTIDPSTARDLDDALSCRQLTDGTFEVGVHIADVSYFVPEGSSLDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMTDKLTFSVIWKLTPEGKILEEWFGRTIIRSCTKLSYDHAQSMIENPTEKIPKEELPPISPEHSIEEVHQAVLNLHSIAKQLRRQRFVDGALRLDQLKLAFTLDHETGLPQGCHIYEYRDSNKLVEEFMLLANMAVAHKIFRTFPEQALLRRHPPPQTKMLSDLVEFCDQMGLPMDVSSAGALNKSLTNTFGDDKYSLARKEVLTNMYSRPMQMALYFCSGMLQDQAQFRHYALNVPLYTHFTSPIRRFADVIVHRLLAAALGYSEQPDVEPDALQKQADHCNDRRMASKRVQELSIGLFFSILVKESGPLESEAMVMGVLNQAFDVLVLRFGVQKRIYCNALALRSYHFQKVGKKPELTLVWEPDDLEEEPIQQVITIFSLVDVVLQAEATALKYSAILKRPGPEHSLGLEKESDEEPED

Target ORF information:

RefSeq Version	NM_001109007.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DIS3-like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109007.2

ORF Insert Sequence:

ATGAACCATC CTGACTACAA GCTGAACCTT CAGTCTCCAG GGACTCCCAG AGGTGTGTCC 
TCTGTGGTTG GCCCGAATGC TGTTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAAG 
TCCATGCGAG GGAAGAAAAA GAGCATATTT GAAACCTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAAAGGAAC GCTTATCCAG GGTGTATTGA GGATCAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC TCCGGATGGT GATCGGGACA TTTTTATTGA TGGAGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGACCTT GTGGTTGTAA AACTGCTTCC TGAGGACCAG 
TGGAAGCCAA GGATAACCTT GTCTCTGCCT GGAGTGCTGG GATTACAGGG AGTTAAACCA 
GAGAGCAACG ACAAAGAAAC AGAAGCTACT AATGAAACTG ACATCTCTGA GGAGTCCTGT 
GGACACCACC TTCTGCAGAA GTCCCCAAAA GGCTGGAATG GTCCTGATGT TATTATAGAG 
GCTCAGTTTG ATGACAGCGA CTCAGAAGAT AGACATGGCA ACACCAGTGG GCTGGTTGAT 
GGTATGAAGA AATTGTCAGT CTGTACTCCT GACAAAGGAA AAGAAGATTC TAGTGCTCCA 
GTTATGAAAG ACGAGAACAC CCCCAAGTCA CAGGACACAA GAGCTTTATC AGAGAAGTCA 
CTCCAGAAAT CAGCAAAGGT GGTTTACATC TTGGAGAAAA AGCATTCCCG AGCAGCAACC 
GGCATCCTGA AACTCTTGGC TGACAAGAAC AGTGACCTGT TTAAGAAATA TGCCCTGTTT 
TCTCCTTCAG ACCACCGAGT ACCTAGAATT TATGTACCAC TCAAGGACTG TCCCCAGGAC 
TTCATGACCC GACCCAAAGA CTATGCCAAC ACGCTGTTCA TCTGCCGCAT CATAGACTGG 
AAGGAGGACT GTAATTTTGC CCTGGGGCAA CTGGCTAAGA GTCTTGGGCA AGCTGGTGAA 
ATTGAACCTG AAACAGAAGG GATCCTGACA GAATATGGTG TGGACTTCTC TGATTTCTCT 
TCAGAAGTTC TCGAATGTCT CCCTCAAAGC CTGCCATGGA CAATTCCACC TGAGGAGGTG 
GGCAAGAGAA GAGACCTAAG AAAAGACTGT ATCTTCACCA TTGACCCATC AACTGCTCGA 
GACCTTGATG ACGCCCTCTC CTGCAGGCAG CTCACTGACG GCACCTTCGA AGTGGGCGTC 
CACATAGCCG ATGTGAGTTA CTTCGTTCCT GAGGGATCCT CTTTGGATAA GGTAGCTGCT 
GAGAGAGCCA CAAGTGTCTA CTTGGTCCAG AAGGTGGTCC CCATGCTTCC CAGGCTTCTG 
TGTGAGGAAC TCTGCAGCCT CAACCCCATG ACTGACAAGC TGACCTTCTC TGTGATCTGG 
AAGCTGACTC CTGAAGGCAA GATCCTTGAG GAGTGGTTTG GCCGCACTAT CATCCGTTCT 
TGCACCAAAT TGAGCTACGA CCATGCGCAG AGCATGATCG AAAATCCGAC TGAGAAGATC 
CCCAAGGAAG AGCTTCCCCC TATTTCTCCA GAGCACAGCA TCGAGGAGGT GCACCAGGCA 
GTCCTGAACC TGCACAGCAT TGCAAAGCAA CTCCGCCGCC AGCGCTTTGT AGATGGTGCA 
CTCCGTTTAG ATCAGCTGAA GCTTGCTTTC ACTCTGGACC ATGAGACTGG ATTGCCTCAA 
GGATGTCATA TCTATGAGTA CCGAGACAGC AACAAGCTTG TAGAGGAGTT CATGCTCCTG 
GCCAACATGG CAGTGGCCCA CAAGATCTTC CGCACCTTCC CTGAGCAGGC CCTGCTGCGC 
CGGCATCCCC CACCGCAGAC GAAGATGCTC AGTGACCTGG TGGAGTTCTG TGACCAGATG 
GGGCTGCCCA TGGATGTCAG CTCTGCAGGG GCCCTAAATA AAAGCCTGAC CAACACATTT 
GGAGATGACA AGTACTCTCT GGCCCGGAAA GAGGTGCTCA CCAACATGTA CTCCCGGCCC 
ATGCAGATGG CACTGTACTT CTGCTCTGGG ATGCTGCAGG ACCAGGCGCA GTTCCGGCAT 
TATGCTCTCA ACGTTCCCCT CTACACACAC TTCACCTCTC CCATCCGCCG CTTTGCTGAC 
GTCATAGTGC ACCGCCTCCT GGCTGCTGCT CTGGGCTACA GTGAGCAGCC AGATGTGGAG 
CCTGATGCCC TACAGAAGCA AGCCGACCAC TGCAATGACC GCCGCATGGC CTCTAAGCGT 
GTGCAGGAGC TCAGCATTGG CCTCTTCTTC TCAATTCTAG TAAAAGAGAG CGGCCCCCTG 
GAGTCTGAAG CCATGGTGAT GGGTGTCCTG AACCAAGCTT TCGATGTGCT GGTACTGCGC 
TTTGGGGTAC AAAAACGCAT CTACTGCAAC GCACTGGCCT TGAGGTCCTA CCACTTCCAG 
AAGGTGGGGA AGAAGCCAGA GCTCACTCTT GTTTGGGAGC CAGATGACCT TGAAGAGGAG 
CCAATACAGC AGGTCATCAC CATCTTCAGC CTGGTGGATG TGGTCCTGCA GGCAGAGGCC 
ACAGCCCTCA AGTACAGTGC TATCCTGAAG CGACCAGGCC CGGAGCATTC CCTGGGTCTG 
GAGAAGGAGT CTGATGAGGA GCCTGAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanism of Dis3l2 substrate recognition in the Lin28-let-7 pathway.
Nature514(7521)252-256(2014 Oct)
Faehnle CR,Walleshauser J,Joshua-Tor L

Mammalian DIS3L2 exoribonuclease targets the uridylated precursors of let-7 miRNAs.
RNA (New York, N.Y.)19(12)1632-8(2013 Dec)
Ustianenko D,Hrossova D,Potesil D,Chalupnikova K,Hrazdilova K,Pachernik J,Cetkovska K,Uldrijan S,Zdrahal Z,Vanacova S

Exonuclease hDIS3L2 specifies an exosome-independent 3'-5' degradation pathway of human cytoplasmic mRNA.
The EMBO journal32(13)1855-68(2013 Jul)
Lubas M,Damgaard CK,Tomecki R,Cysewski D,Jensen TH,Dziembowski A

A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.
Nature497(7448)244-8(2013 May)
Chang HM,Triboulet R,Thornton JE,Gregory RI

Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility.
Nature genetics44(3)277-84(2012 Feb)
Astuti D,Morris MR,Cooper WN,Staals RH,Wake NC,Fews GA,Gill H,Gentle D,Shuib S,Ricketts CJ,Cole T,van Essen AJ,van Lingen RA,Neri G,Opitz JM,Rump P,Stolte-Dijkstra I,Müller F,Pruijn GJ,Latif F,Maher ER

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Dis3l2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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