Pcif1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcif1
Entrez Gene ID	362269
Full Name	PDX1 C-terminal inhibiting factor 1
Synonyms	RGD1310800
General protein information	Preferred Names PDX1 C-terminal inhibiting factor 1 Names phosphorylated CTD-interacting factor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42

mRNA and Protein(s)

mRNA	Protein	Name
NM_001108605.1	NP_001102075.1	phosphorylated CTD-interacting factor 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PCIF1	NP_071387.1
Canis lupus familiaris (dog)	PCIF1	XP_534442.2
Rattus norvegicus (Norway rat)	Pcif1	NP_001102075.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011933	XP_320600.4
Mus musculus (house mouse)	Pcif1	NP_666241.1
Bos taurus (cattle)	PCIF1	NP_001096569.1
Danio rerio (zebrafish)	pcif1	XP_688220.3
Gallus gallus (chicken)	PCIF1	XP_001234172.2
Drosophila melanogaster (fruit fly)	CG11399	NP_649254.2
Xenopus tropicalis (tropical clawed frog)	pcif1	NP_001017341.1
Pan troglodytes (chimpanzee)	PCIF1	XP_514687.2

Gene Ontology
Bibliography

Related articles in PubMed

Identification of the m
Boulias K, Toczydłowska-Socha D, Hawley BR, Liberman N, Takashima K, Zaccara S, Guez T, Vasseur JJ, Debart F, Aravind L, Jaffrey SR, Greer EL
Molecular cell75(3)631-643.e8(2019 08)

PCIF1 Catalyzes m6Am mRNA Methylation to Regulate Gene Expression.
Sendinc E, Valle-Garcia D, Dhall A, Chen H, Henriques T, Navarrete-Perea J, Sheng W, Gygi SP, Adelman K, Shi Y
Molecular cell75(3)620-630.e9(2019 08)

Cap-specific, terminal N
Sun H, Zhang M, Li K, Bai D, Yi C
Cell research29(1)80-82(2019 01)

Cap-specific terminal
Akichika S, Hirano S, Shichino Y, Suzuki T, Nishimasu H, Ishitani R, Sugita A, Hirose Y, Iwasaki S, Nureki O, Suzuki T
Science (New York, N.Y.)363(6423)(2019 01)

Docking motif-guided mapping of the interactome of protein phosphatase-1.
Hendrickx A, Beullens M, Ceulemans H, Den Abt T, Van Eynde A, Nicolaescu E, Lesage B, Bollen M
Chemistry & biology16(4)365-71(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pcif1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcif1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07903	NM_001108605.1 Latest version!	Rattus norvegicus PDX1 C-terminal inhibiting factor 1 (Pcif1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcif1 ( NM_001108605.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102075.1 Rattus norvegicus PDX1 C-terminal inhibiting factor 1 (Pcif1), mRNA.

Price & Availability↑

CloneID	ORa07903
Clone ID Related Accession (Same CDS sequence)	NM_001108605.1
Accession Version	NM_001108605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570636800000
Organism	Rattus norvegicus(Norway rat)
Product	phosphorylated CTD-interacting factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474005.2. On or before Oct 4, 2007 this sequence version replaced XM_342577.3, XM_001070779.1. ##Evidence-Data-START## Transcript exon combination :: FQ225793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAATG AGAATCACGG CAGCCCCCGG GAAGAAGCAT CCCTTCTAAG TCACTCTCCA 
GGCACCTCCA ATCAGAACCA GCCCTGTTCT CCAAAGCCAG TTCGCCTGGT ACAGGACCTC 
CCAGAGGAAC TGGTACATGC AGGCTGGGAG AAGTGCTGGA GCCGAAGGGA GAGCCGTCCC 
TACTACTTCA ACCGATTCAC CAACCAGTCC CTGTGGGAGA TGCCTGTGCT GGGTCAGCAC 
GATGTGCTTT CGGACCCTTT GGGGCTAAAT GCAACCCCAC TGCCCCAAGA CTCAACCTTA 
GTGGAAACTC CCCCAGTAGA GAACAAGCCC AGAAAGCGAC AGCTCTCAGA AGAGCAGCCA 
AGTGGCAATG GAGTGAAGAA GCCCAAGATT GAAATACCTG TGACACCCAC AAGCCAGTCG 
GTGCCCAGTT CCCCCAGCAT CCCAGGAACC CCAATACTAA AGATTTGGGG TGCATCCACT 
GAAGATAAAC AAGCAGCTCT CCTCCGACCC ACTGAGGTGT ACTGGGACCT GGACATCCAG 
ACCAATGCTG TCATCAAGCA CAGGGGTCCT TCTGAGGTCC TGCCCCCACA CCCTGAGGTT 
GAGCTGCTCC GCTCTCAGCT CATCTTGAAG CTGCGCCAGC ACTACCGGGA ACTGTGCCAG 
CAGCGGGAGG GCATTGAGCC ACCCCGGGAA TCTTTCAACC GCTGGATGCT AGAGCGCAAA 
GTGGTGGACA AAGGCTGTGA TCCTCTGTTG CCAAGCAACT GTGAACCAGT TGTGTCTCCG 
TCCATGTTTC GTGAAATTAT GAATGACATT CCCATCAGGT TATCTCGAAT CAAGTTCCGG 
GAGGAAGCCA AACGCCTGCT CTTTAAATAT GCAGAAGCTG CCAGGCGGCT CATTGAGTCC 
AGGAGTGCAT CCCCTGACAG CAGAAAAGTG GTCAAGTGGA ATGTGGAGGA CACCTTTAGC 
TGGCTGCGGA AGGAGCACTC AGCCTCGAAG GAGGACTACA TGGACCGCCT GGAGCACCTG 
CGGAGGCAGT GTGGGCCCCA TGTCTCTGCT GCAGCCAAGG ACTCTGTGGA GGGCATCTGC 
AGCAAGATCT ACCACATCTC CCTGGAATAT GTTAAACGGA TCCGAGAGAA GCACCTTGCC 
ATCCTCAAGG AGAACAACAT CCCAGAGGAA GTAGAGGCCT CAGAGTTGGA GCCCCGCCTG 
GTATACTGCT ACCCAGTGCG CCTGGCCGTG TCTGCACCCC CCATGCCTAG TGTGGAGATG 
CACATGGAGA ACAGTGTAGT CTGCATCCGC TACAAGGGAG AGATGGTCAA GGTCAGCCGC 
AGCTACTTCA GCAAACTGTG GCTCCTCTAC CGCTACAGCT GTGTGGATGA CTCTGCCTTT 
GAGAGGTTCC TGCCTAGAGT CTGGTGTCTT CTCCGTCGAT ATCAGATGAT GTTTGGTGTG 
GGCCTCTATG AGGGGACTGG CCTGCAGGGG TCACTGCCAG TGCACGTCTT TGAGGCCCTC 
CACAGACTCT TTGGTGTCAG TTTCGAGTGC TTCGCCTCAC CCCTCAACTG CTACTTCCGC 
CAGTACTGTT CTGCCTTCCC TGACACAGAT GGCTACTTTG GCTCCCGCGG GCCCTGCCTG 
GACTTCACCC CGCTGAGTGG TTCCTTTGAG GCCAACCCTC CGTTCTGCGA GGAGCTCATG 
GATGCTATGG TCTCTCACTT CGAGAAACTG CTGGAGAGCT CACCAGAACC CCTGTCCTTC 
ATTGTGTTCA TCCCGGAGTG GCGGGAGCCC CCAACACCAG CGCTCACCCG AATGGAGCAG 
AGCCGCTTCA AACGCCACCA GCTGGTCTTG TCTGCCTTTG AGCACGAGTA CCGCAGTGGC 
TCCCAGCACA TCTGCAAGAA GGAGGAAATG CACTACAAGG CCGTCCACAA CACGGCAGTG 
CTCTTCTTGC AGAATGACCC CGGGTTCGCC AAGTGGGGGC CGACGCCAGA GCGGCTGCAG 
GAGCTCACCG CTGCTTATAA GCAGTCGGGC CGCAGCCATG GCTCCAGCTC TTCCTCCTCC 
TCCTCCTCCT CAGAGGCCAA GGACCGGGAC TCCGGCCGGG AGCAGGGCCC TAGTCGAGAG 
CCTCACCCCA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102075.1
CDS	1515..3629
Translation	MANENHGSPREEASLLSHSPGTSNQNQPCSPKPVRLVQDLPEELVHAGWEKCWSRRESRPYYFNRFTNQSLWEMPVLGQHDVLSDPLGLNATPLPQDSTLVETPPVENKPRKRQLSEEQPSGNGVKKPKIEIPVTPTSQSVPSSPSIPGTPILKIWGASTEDKQAALLRPTEVYWDLDIQTNAVIKHRGPSEVLPPHPEVELLRSQLILKLRQHYRELCQQREGIEPPRESFNRWMLERKVVDKGCDPLLPSNCEPVVSPSMFREIMNDIPIRLSRIKFREEAKRLLFKYAEAARRLIESRSASPDSRKVVKWNVEDTFSWLRKEHSASKEDYMDRLEHLRRQCGPHVSAAAKDSVEGICSKIYHISLEYVKRIREKHLAILKENNIPEEVEASELEPRLVYCYPVRLAVSAPPMPSVEMHMENSVVCIRYKGEMVKVSRSYFSKLWLLYRYSCVDDSAFERFLPRVWCLLRRYQMMFGVGLYEGTGLQGSLPVHVFEALHRLFGVSFECFASPLNCYFRQYCSAFPDTDGYFGSRGPCLDFTPLSGSFEANPPFCEELMDAMVSHFEKLLESSPEPLSFIVFIPEWREPPTPALTRMEQSRFKRHQLVLSAFEHEYRSGSQHICKKEEMHYKAVHNTAVLFLQNDPGFAKWGPTPERLQELTAAYKQSGRSHGSSSSSSSSSSEAKDRDSGREQGPSREPHPT

Target ORF information:

RefSeq Version	NM_001108605.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PDX1 C-terminal inhibiting factor 1 (Pcif1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108605.1

ORF Insert Sequence:

ATGGCCAATG AGAATCACGG CAGCCCCCGG GAAGAAGCAT CCCTTCTAAG TCACTCTCCA 
GGCACCTCCA ATCAGAACCA GCCCTGTTCT CCAAAGCCAG TTCGCCTGGT ACAGGACCTC 
CCAGAGGAAC TGGTACATGC AGGCTGGGAG AAGTGCTGGA GCCGAAGGGA GAGCCGTCCC 
TACTACTTCA ACCGATTCAC CAACCAGTCC CTGTGGGAGA TGCCTGTGCT GGGTCAGCAC 
GATGTGCTTT CGGACCCTTT GGGGCTAAAT GCAACCCCAC TGCCCCAAGA CTCAACCTTA 
GTGGAAACTC CCCCAGTAGA GAACAAGCCC AGAAAGCGAC AGCTCTCAGA AGAGCAGCCA 
AGTGGCAATG GAGTGAAGAA GCCCAAGATT GAAATACCTG TGACACCCAC AAGCCAGTCG 
GTGCCCAGTT CCCCCAGCAT CCCAGGAACC CCAATACTAA AGATTTGGGG TGCATCCACT 
GAAGATAAAC AAGCAGCTCT CCTCCGACCC ACTGAGGTGT ACTGGGACCT GGACATCCAG 
ACCAATGCTG TCATCAAGCA CAGGGGTCCT TCTGAGGTCC TGCCCCCACA CCCTGAGGTT 
GAGCTGCTCC GCTCTCAGCT CATCTTGAAG CTGCGCCAGC ACTACCGGGA ACTGTGCCAG 
CAGCGGGAGG GCATTGAGCC ACCCCGGGAA TCTTTCAACC GCTGGATGCT AGAGCGCAAA 
GTGGTGGACA AAGGCTGTGA TCCTCTGTTG CCAAGCAACT GTGAACCAGT TGTGTCTCCG 
TCCATGTTTC GTGAAATTAT GAATGACATT CCCATCAGGT TATCTCGAAT CAAGTTCCGG 
GAGGAAGCCA AACGCCTGCT CTTTAAATAT GCAGAAGCTG CCAGGCGGCT CATTGAGTCC 
AGGAGTGCAT CCCCTGACAG CAGAAAAGTG GTCAAGTGGA ATGTGGAGGA CACCTTTAGC 
TGGCTGCGGA AGGAGCACTC AGCCTCGAAG GAGGACTACA TGGACCGCCT GGAGCACCTG 
CGGAGGCAGT GTGGGCCCCA TGTCTCTGCT GCAGCCAAGG ACTCTGTGGA GGGCATCTGC 
AGCAAGATCT ACCACATCTC CCTGGAATAT GTTAAACGGA TCCGAGAGAA GCACCTTGCC 
ATCCTCAAGG AGAACAACAT CCCAGAGGAA GTAGAGGCCT CAGAGTTGGA GCCCCGCCTG 
GTATACTGCT ACCCAGTGCG CCTGGCCGTG TCTGCACCCC CCATGCCTAG TGTGGAGATG 
CACATGGAGA ACAGTGTAGT CTGCATCCGC TACAAGGGAG AGATGGTCAA GGTCAGCCGC 
AGCTACTTCA GCAAACTGTG GCTCCTCTAC CGCTACAGCT GTGTGGATGA CTCTGCCTTT 
GAGAGGTTCC TGCCTAGAGT CTGGTGTCTT CTCCGTCGAT ATCAGATGAT GTTTGGTGTG 
GGCCTCTATG AGGGGACTGG CCTGCAGGGG TCACTGCCAG TGCACGTCTT TGAGGCCCTC 
CACAGACTCT TTGGTGTCAG TTTCGAGTGC TTCGCCTCAC CCCTCAACTG CTACTTCCGC 
CAGTACTGTT CTGCCTTCCC TGACACAGAT GGCTACTTTG GCTCCCGCGG GCCCTGCCTG 
GACTTCACCC CGCTGAGTGG TTCCTTTGAG GCCAACCCTC CGTTCTGCGA GGAGCTCATG 
GATGCTATGG TCTCTCACTT CGAGAAACTG CTGGAGAGCT CACCAGAACC CCTGTCCTTC 
ATTGTGTTCA TCCCGGAGTG GCGGGAGCCC CCAACACCAG CGCTCACCCG AATGGAGCAG 
AGCCGCTTCA AACGCCACCA GCTGGTCTTG TCTGCCTTTG AGCACGAGTA CCGCAGTGGC 
TCCCAGCACA TCTGCAAGAA GGAGGAAATG CACTACAAGG CCGTCCACAA CACGGCAGTG 
CTCTTCTTGC AGAATGACCC CGGGTTCGCC AAGTGGGGGC CGACGCCAGA GCGGCTGCAG 
GAGCTCACCG CTGCTTATAA GCAGTCGGGC CGCAGCCATG GCTCCAGCTC TTCCTCCTCC 
TCCTCCTCCT CAGAGGCCAA GGACCGGGAC TCCGGCCGGG AGCAGGGCCC TAGTCGAGAG 
CCTCACCCCA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of the m
Molecular cell75(3)631-643.e8(2019 08)
Boulias K,Toczydłowska-Socha D,Hawley BR,Liberman N,Takashima K,Zaccara S,Guez T,Vasseur JJ,Debart F,Aravind L,Jaffrey SR,Greer EL

PCIF1 Catalyzes m6Am mRNA Methylation to Regulate Gene Expression.
Molecular cell75(3)620-630.e9(2019 08)
Sendinc E,Valle-Garcia D,Dhall A,Chen H,Henriques T,Navarrete-Perea J,Sheng W,Gygi SP,Adelman K,Shi Y

Cap-specific, terminal N
Cell research29(1)80-82(2019 01)
Sun H,Zhang M,Li K,Bai D,Yi C

Cap-specific terminal
Science (New York, N.Y.)363(6423)(2019 01)
Akichika S,Hirano S,Shichino Y,Suzuki T,Nishimasu H,Ishitani R,Sugita A,Hirose Y,Iwasaki S,Nureki O,Suzuki T

Docking motif-guided mapping of the interactome of protein phosphatase-1.
Chemistry & biology16(4)365-71(2009 Apr)
Hendrickx A,Beullens M,Ceulemans H,Den Abt T,Van Eynde A,Nicolaescu E,Lesage B,Bollen M

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Pcif1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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