Ankrd31 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ankrd31
Entrez Gene ID	100359720
Full Name	ankyrin repeat domain 31
General protein information	Preferred Names ankyrin repeat domain 31 Names LOW QUALITY PROTEIN: putative ankyrin repeat domain-containing protein 31 putative ankyrin repeat domain-containing protein 31 rCG64386-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_017591363.1	XP_017446852.1	LOW QUALITY PROTEIN: putative ankyrin repeat domain-containing protein 31 isoform X1
XM_008775069.2	XP_008773291.1	putative ankyrin repeat domain-containing protein 31 isoform X2

Pathways from BioSystems
Gene Ontology

The following Ankrd31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ankrd31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa50710	XM_008775069.2 Latest version!	Rattus norvegicus ankyrin repeat domain 31 (Ankrd31), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa59980	XM_017591363.1 Latest version!	Rattus norvegicus ankyrin repeat domain 31 (Ankrd31), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ankrd31 ( XM_008775069.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008773291.1 Rattus norvegicus ankyrin repeat domain 31 (Ankrd31), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa50710
Clone ID Related Accession (Same CDS sequence)	XM_008775069.2
Accession Version	XM_008775069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	putative ankyrin repeat domain-containing protein 31 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000070.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008775069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAGAAAG GTGACCAGGC TTCCGACTGT GACAGTGATG AAACAGTGAT TGAGGGGTCG 
GTGACAGAGA ACGAACTGGA AGATGATGAG CTTCCCTGGA GAAGGTTCTT GAGTCAAGAT 
ACAACTTTTA CATCTGAGTT CTGTTTTCAT TCTGGTATAA ATGGAACACA GAATGGTATG 
CATTCTCCTG AGATTCAACT TGGACTGAAA CTCAGAAAGG ATTCACAAGA GCAAAATGAT 
AAAAATGAGG TGTTGCGGGC TCTTAGTGAG GACCTAGTGT TACAGGATCC TCAGGATGAA 
ACAACACAAA ATCAAGTTCT GTTACAAACT AGAAAAGAAT TGCCAGTATT CTCTGTGTCA 
GTTCCTCACC CCGAAGTTTC ATGGAGTCAC CAAACTACTG GAGGGCCTGG AGCTGAAATC 
TGTGAACATT TGCCACACTC AGAAAAGGAA CTAAGTGAAA ACTCAGATTC CCCTGAAGTT 
AGTCTTCTCT CAGGGACTTC TCTTATGGCA TCTGATTTGG TTACCTTAAA GGAACAATTA 
ACAGAGCCAG TGAAGACCTT AGCAGTACCA AACACATTTT CTGAGCCTGG AAAGAAAGTA 
ACACTGACCT TGACTTCTAT AGAAACCAAA GATGAAGAAA GCTCTCTTGA AACATTTGTG 
TCTGCATTAG AAAAGTTACT AGAATCACCA GAATATACCC AAGAAGAAAG ACTGCTTGAA 
ATAATGAATG ACTTCAGTCC TCAAGAGTTC TTGACCTCAT TGAGCAATTC CATGAGTTCT 
GTGTCTGTCC CTTCGAGTGC CTTGTCAGCA GGTGATAGAG ATGAACTAGG AAACAAGGCT 
GGTGCTGTGT TGCCAGCTCA ATTGCTGGCA GCCATCAACA CATTGCCAGG TGCTGATGTG 
GGACCCATCT GTCAAGGACA AGAGAGAAGC AGCAGTGTTA GTGGTGGAAA TGGATGTTTA 
GAAGTGCAGC CTGCCATGTC TCAGAAAGAT GAAGATTGTA CACACATAGC ACAGAATATA 
GAAGACCCAA AACCATTTAG ATTACAAACA TTGACTCATG AAAATGCCAC CTCTTATGAG 
CAACTAAACA AAAAGGAAAA TTCAGATTCC ATGAAAAACA CCTCTACCCA GAAAACACCT 
CGTGTACTAA GAAGATCATC TAGACTGGAA AAACTAAGAG CAAGCAGAGA TGTGCATACA 
GATGTCATAC TGGAGAGGCC AGAGAGGATT TTATCAGAAG CACTTAGCTG TAAGGATCGA 
ATAAATAGCA TATTTACAAC TGAGAATTTC AGTAAAAGGA AGAACATGCA TTCATCCAGA 
TTCAAGAATG AACAGATAAG GAAAAATGAA CAACTTAGAA TAAAAAATGA AGTGAACACA 
GCTGGAATCA ATAAGAGAAA TGCCAAAGGA GAAAGCCGGC TCCATGTGGC TTCCAAAGGA 
GGAAATCTTT CCCTAGTGAA AATGCTAATA GAATCTGGAG CAGATGTGAA TCTCAAAGAT 
AATGCAGGTT GGACACCCCT TCATAAGGCA TCAAGTGGGG GATTTGATGA TATAATTATA 
GAGTTATTAA AAGCTGGTGC AAATGTGAAC TGTGAAAATG TAGATGGGAT TTTGCCTTTA 
CATGGTGCTT CTGCAGGGAA CCATTTAAAG GCAGCTGAGA TACTCCTAGA ACATGGAGCA 
AACCCTAGTC AGAAAGATCA GAAACAGAGG ACTGCTTTGG ATGAAGCAGA TGATGAAAAA 
ATGAAAGAAC TACTAAAATC TCATGGTGCT ATTGAGACCG CTCATGGAGA AGAGAGGAGC 
TCCATAGTTC CAGTAAAAAT TCCTGCTATT CGCCCAAAAA GATATAAGCC TTTTATCTGT 
GACAATGACA AAGCAGTTGG TTCTCCTGCT CCCTCACATA AAGCAAAGAG GAGTGAGAGC 
CGACCTGTGC ATCAGACCAT CAGTGCCATT CTAAAAGATC TAGAAGAAAA ACAGGAAACC 
TTACTGAAGT TTGAGATAAG AAACTCTGAA GATGAAGAAC AATACATTGA AAAGATGTTA 
GACATAAAAG AAGTTATGGA TAATATACTT GCTCAACAGA AAACAGAAAG GGATGATCTA 
GCTAAAAAAT ACAGGGTATC CATGGAGTCA TTTAAACATG GGGCCCTGAG AGAACAGCTG 
GCTAACTTGG CCACAAGACA AAAGAGTCTT TTAGTTGTAG CAAAGAAACA AAAAAAGATA 
AGACTGAAAA TTCAAAACTA TAAGACTGCC ACACCATCAT CTGGTAATAA TTTAAGGAAG 
CTGCCATGTA ACTCAGACAT CTCCAGTGAC AAGACCAGCC AATTTGATGC TCCTTCACTA 
ACAGGCACCA TAAATATTTC AGAAGATAAA AGTATATTTT CCCAAAATGA TGTCTGTCTA 
CCGGCTTTTC CTCAGAATCA GGGCCTTTCC CGTTGTAAAC CAAAAAGAAG AAACAAGAGA 
ACAGGTTCCC AACAACCATC TGAGGGAGCA TCTAAGCCTC TGGCGCAGAC TGTTGCTATC 
TTAGATACCT ATACTGCGCG TCAGACAAAG CCGTGTCTTA AGAAATCAGC TTCTGTTGCT 
TTGAATGCAG ACAGCATGCA AATCTCTCCT CCCTCTGTGT CTGCCCATCA ACCTTCTTCT 
AAAAAGGCTC CACACCACAG CACAACTCCC AGAAAGAAAA CTGTGCAGCT GAAAGACTTG 
ATTTTACTTG GAAGAATTAA TCCAGGAAAT AACATTCTGG AATTTAAAAC CCAGGAGACA 
ACCCACAGAG CCAGTGTTTT ACTGAGTGGT AAACTGAAGG TAGAAAATGG TCAGATTTAC 
CAGAACCCAG TTACCTGGCT CAAGGATGTC CTGGGAGGTG GCAGTTATGT GACCTGGAAT 
TACGCTTGGA ATAAGGTAAC ATACCTTGGA AAGGAGCTTT TGAAGTATGT TTCTGAAGCA 
CCTCCTGTTT CTGCAGAACC GAGTAGCACA CCTCAGCAGC ACCAGCCTTG CCTCTCAGCA 
GGCACTTCCA GAGAGTCGAT GCAAATCATC CCTCATTATC TACAGATAAA AGAAATACTT 
CTAATCAGCG ATCAAGAGCT TCTGCCGTGC CATGTAATGG AGCAGCATTG GAAGTTCTAT 
GTGGAATGTA AGAAGCTATC GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008773291.1
CDS	114..3260
Translation	MEKGDQASDCDSDETVIEGSVTENELEDDELPWRRFLSQDTTFTSEFCFHSGINGTQNGMHSPEIQLGLKLRKDSQEQNDKNEVLRALSEDLVLQDPQDETTQNQVLLQTRKELPVFSVSVPHPEVSWSHQTTGGPGAEICEHLPHSEKELSENSDSPEVSLLSGTSLMASDLVTLKEQLTEPVKTLAVPNTFSEPGKKVTLTLTSIETKDEESSLETFVSALEKLLESPEYTQEERLLEIMNDFSPQEFLTSLSNSMSSVSVPSSALSAGDRDELGNKAGAVLPAQLLAAINTLPGADVGPICQGQERSSSVSGGNGCLEVQPAMSQKDEDCTHIAQNIEDPKPFRLQTLTHENATSYEQLNKKENSDSMKNTSTQKTPRVLRRSSRLEKLRASRDVHTDVILERPERILSEALSCKDRINSIFTTENFSKRKNMHSSRFKNEQIRKNEQLRIKNEVNTAGINKRNAKGESRLHVASKGGNLSLVKMLIESGADVNLKDNAGWTPLHKASSGGFDDIIIELLKAGANVNCENVDGILPLHGASAGNHLKAAEILLEHGANPSQKDQKQRTALDEADDEKMKELLKSHGAIETAHGEERSSIVPVKIPAIRPKRYKPFICDNDKAVGSPAPSHKAKRSESRPVHQTISAILKDLEEKQETLLKFEIRNSEDEEQYIEKMLDIKEVMDNILAQQKTERDDLAKKYRVSMESFKHGALREQLANLATRQKSLLVVAKKQKKIRLKIQNYKTATPSSGNNLRKLPCNSDISSDKTSQFDAPSLTGTINISEDKSIFSQNDVCLPAFPQNQGLSRCKPKRRNKRTGSQQPSEGASKPLAQTVAILDTYTARQTKPCLKKSASVALNADSMQISPPSVSAHQPSSKKAPHHSTTPRKKTVQLKDLILLGRINPGNNILEFKTQETTHRASVLLSGKLKVENGQIYQNPVTWLKDVLGGGSYVTWNYAWNKVTYLGKELLKYVSEAPPVSAEPSSTPQQHQPCLSAGTSRESMQIIPHYLQIKEILLISDQELLPCHVMEQHWKFYVECKKLSF

Target ORF information:

RefSeq Version	XM_008775069.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat domain 31 (Ankrd31), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008775069.2

ORF Insert Sequence:

ATGGAGAAAG GTGACCAGGC TTCCGACTGT GACAGTGATG AAACAGTGAT TGAGGGGTCG 
GTGACAGAGA ACGAACTGGA AGATGATGAG CTTCCCTGGA GAAGGTTCTT GAGTCAAGAT 
ACAACTTTTA CATCTGAGTT CTGTTTTCAT TCTGGTATAA ATGGAACACA GAATGGTATG 
CATTCTCCTG AGATTCAACT TGGACTGAAA CTCAGAAAGG ATTCACAAGA GCAAAATGAT 
AAAAATGAGG TGTTGCGGGC TCTTAGTGAG GACCTAGTGT TACAGGATCC TCAGGATGAA 
ACAACACAAA ATCAAGTTCT GTTACAAACT AGAAAAGAAT TGCCAGTATT CTCTGTGTCA 
GTTCCTCACC CCGAAGTTTC ATGGAGTCAC CAAACTACTG GAGGGCCTGG AGCTGAAATC 
TGTGAACATT TGCCACACTC AGAAAAGGAA CTAAGTGAAA ACTCAGATTC CCCTGAAGTT 
AGTCTTCTCT CAGGGACTTC TCTTATGGCA TCTGATTTGG TTACCTTAAA GGAACAATTA 
ACAGAGCCAG TGAAGACCTT AGCAGTACCA AACACATTTT CTGAGCCTGG AAAGAAAGTA 
ACACTGACCT TGACTTCTAT AGAAACCAAA GATGAAGAAA GCTCTCTTGA AACATTTGTG 
TCTGCATTAG AAAAGTTACT AGAATCACCA GAATATACCC AAGAAGAAAG ACTGCTTGAA 
ATAATGAATG ACTTCAGTCC TCAAGAGTTC TTGACCTCAT TGAGCAATTC CATGAGTTCT 
GTGTCTGTCC CTTCGAGTGC CTTGTCAGCA GGTGATAGAG ATGAACTAGG AAACAAGGCT 
GGTGCTGTGT TGCCAGCTCA ATTGCTGGCA GCCATCAACA CATTGCCAGG TGCTGATGTG 
GGACCCATCT GTCAAGGACA AGAGAGAAGC AGCAGTGTTA GTGGTGGAAA TGGATGTTTA 
GAAGTGCAGC CTGCCATGTC TCAGAAAGAT GAAGATTGTA CACACATAGC ACAGAATATA 
GAAGACCCAA AACCATTTAG ATTACAAACA TTGACTCATG AAAATGCCAC CTCTTATGAG 
CAACTAAACA AAAAGGAAAA TTCAGATTCC ATGAAAAACA CCTCTACCCA GAAAACACCT 
CGTGTACTAA GAAGATCATC TAGACTGGAA AAACTAAGAG CAAGCAGAGA TGTGCATACA 
GATGTCATAC TGGAGAGGCC AGAGAGGATT TTATCAGAAG CACTTAGCTG TAAGGATCGA 
ATAAATAGCA TATTTACAAC TGAGAATTTC AGTAAAAGGA AGAACATGCA TTCATCCAGA 
TTCAAGAATG AACAGATAAG GAAAAATGAA CAACTTAGAA TAAAAAATGA AGTGAACACA 
GCTGGAATCA ATAAGAGAAA TGCCAAAGGA GAAAGCCGGC TCCATGTGGC TTCCAAAGGA 
GGAAATCTTT CCCTAGTGAA AATGCTAATA GAATCTGGAG CAGATGTGAA TCTCAAAGAT 
AATGCAGGTT GGACACCCCT TCATAAGGCA TCAAGTGGGG GATTTGATGA TATAATTATA 
GAGTTATTAA AAGCTGGTGC AAATGTGAAC TGTGAAAATG TAGATGGGAT TTTGCCTTTA 
CATGGTGCTT CTGCAGGGAA CCATTTAAAG GCAGCTGAGA TACTCCTAGA ACATGGAGCA 
AACCCTAGTC AGAAAGATCA GAAACAGAGG ACTGCTTTGG ATGAAGCAGA TGATGAAAAA 
ATGAAAGAAC TACTAAAATC TCATGGTGCT ATTGAGACCG CTCATGGAGA AGAGAGGAGC 
TCCATAGTTC CAGTAAAAAT TCCTGCTATT CGCCCAAAAA GATATAAGCC TTTTATCTGT 
GACAATGACA AAGCAGTTGG TTCTCCTGCT CCCTCACATA AAGCAAAGAG GAGTGAGAGC 
CGACCTGTGC ATCAGACCAT CAGTGCCATT CTAAAAGATC TAGAAGAAAA ACAGGAAACC 
TTACTGAAGT TTGAGATAAG AAACTCTGAA GATGAAGAAC AATACATTGA AAAGATGTTA 
GACATAAAAG AAGTTATGGA TAATATACTT GCTCAACAGA AAACAGAAAG GGATGATCTA 
GCTAAAAAAT ACAGGGTATC CATGGAGTCA TTTAAACATG GGGCCCTGAG AGAACAGCTG 
GCTAACTTGG CCACAAGACA AAAGAGTCTT TTAGTTGTAG CAAAGAAACA AAAAAAGATA 
AGACTGAAAA TTCAAAACTA TAAGACTGCC ACACCATCAT CTGGTAATAA TTTAAGGAAG 
CTGCCATGTA ACTCAGACAT CTCCAGTGAC AAGACCAGCC AATTTGATGC TCCTTCACTA 
ACAGGCACCA TAAATATTTC AGAAGATAAA AGTATATTTT CCCAAAATGA TGTCTGTCTA 
CCGGCTTTTC CTCAGAATCA GGGCCTTTCC CGTTGTAAAC CAAAAAGAAG AAACAAGAGA 
ACAGGTTCCC AACAACCATC TGAGGGAGCA TCTAAGCCTC TGGCGCAGAC TGTTGCTATC 
TTAGATACCT ATACTGCGCG TCAGACAAAG CCGTGTCTTA AGAAATCAGC TTCTGTTGCT 
TTGAATGCAG ACAGCATGCA AATCTCTCCT CCCTCTGTGT CTGCCCATCA ACCTTCTTCT 
AAAAAGGCTC CACACCACAG CACAACTCCC AGAAAGAAAA CTGTGCAGCT GAAAGACTTG 
ATTTTACTTG GAAGAATTAA TCCAGGAAAT AACATTCTGG AATTTAAAAC CCAGGAGACA 
ACCCACAGAG CCAGTGTTTT ACTGAGTGGT AAACTGAAGG TAGAAAATGG TCAGATTTAC 
CAGAACCCAG TTACCTGGCT CAAGGATGTC CTGGGAGGTG GCAGTTATGT GACCTGGAAT 
TACGCTTGGA ATAAGGTAAC ATACCTTGGA AAGGAGCTTT TGAAGTATGT TTCTGAAGCA 
CCTCCTGTTT CTGCAGAACC GAGTAGCACA CCTCAGCAGC ACCAGCCTTG CCTCTCAGCA 
GGCACTTCCA GAGAGTCGAT GCAAATCATC CCTCATTATC TACAGATAAA AGAAATACTT 
CTAATCAGCG ATCAAGAGCT TCTGCCGTGC CATGTAATGG AGCAGCATTG GAAGTTCTAT 
GTGGAATGTA AGAAGCTATC GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd31 ( XM_017591363.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446852.1 Rattus norvegicus ankyrin repeat domain 31 (Ankrd31), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa59980
Clone ID Related Accession (Same CDS sequence)	XM_017591363.1
Accession Version	XM_017591363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	LOW QUALITY PROTEIN: putative ankyrin repeat domain-containing protein 31 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAGAAAG GTGACCAGGC TTCCGACTGT GACAGTGATG AAACAGTGAT TGAGGGGTCG 
GTGACAGAGA ACGAACTGGA AGATGATGAG CTTCCCTGGA GAAGGTTCTT GAGTCAAGAT 
ACAACTTTTA CATCTGAGTT CTGTTTTCAT TCTGGTATAA ATGGAACACA GAATGGTATG 
CATTCTCCTG AGATTCAACT TGGACTGAAA CTCAGAAAGG ATTCACAAGA GCAAAATGAT 
AAAAATGAGG TGTTGCGGGC TCTTAGTGAG GACCTAGTGT TACAGGATCC TCAGGATGAA 
ACAACACAAA ATCAAGTTCT GTTACAAACT AGAAAAGAAT TGCCAGTATT TTCTCTGTGT 
CAGTTCCTCA CCGAGTTTCA ATGGGTCACC AAAACTACTG GAGATACATA TTGTTCTATT 
CTAGGGCCTG GAGCTGAAAT CTGTGAACAT TTGCCACACT CAGAAAAGGA ACTAAGTGAA 
AACTCAGATT CCCCTGAAGT TAGTCTTCTC TCAGGGACTT CTCTTATGGC ATCTGATTTG 
GTTACCTTAA AGGAACAATT AACAGAGCCA GTGAAGACCT TAGCAGTACC AAACACATTT 
TCTGAGCCTG GAAAGAAAGT AACACTGACC TTGACTTCTA TAGAAACCAA AGATGAAGAA 
AGCTCTCTTG AAACATTTGT GTCTGCATTA GAAAAGTTAC TAGAATCACC AGAATATACC 
CAAGAAGAAA GACTGCTTGA AATAATGAAT GACTTCAGTC CTCAAGAGTT CTTGACCTCA 
TTGAGCAATT CCATGAGTTC TGTGTCTGTC CCTTCGAGTG CCTTGTCAGC AGGTGATAGA 
GATGAACTAG GAAACAAGGC TGGTGCTGTG TTGCCAGCTC AATTGCTGGC AGCCATCAAC 
ACATTGCCAG GTGCTGATGT GGGACCCATC TGTCAAGGAC AAGAGAGAAG CAGCAGTGTT 
AGTGGTGGAA ATGGATGTTT AGAAGTGCAG CCTGCCATGT CTCAGAAAGA TGAAGATTGT 
ACACACATAG CACAGAATAT AGAAGACCCA AAACCATTTA GATTACAAAC ATTGACTCAT 
GAAAATGCCA CCTCTTATGA GCAACTAAAC AAAAAGGAAA ATTCAGATTC CATGAAAAAC 
ACCTCTACCC AGAAAACACC TCGTGTACTA AGAAGATCAT CTAGACTGGA AAAACTAAGA 
GCAAGCAGAG ATGTGCATAC AGATGTCATA CTGGAGAGGC CAGAGAGGAT TTTATCAGAA 
GCACTTAGCT GTAAGGATCG AATAAATAGC ATATTTACAA CTGAGAATTT CAGTAAAAGG 
AAGAACATGC ATTCATCCAG ATTCAAGAAT GAACAGATAA GGAAAAATGA ACAACTTAGA 
ATAAAAAATG AAGTGAACAC AGCTGGAATC AATAAGAGAA ATGCCAAAGG AGAAAGCCGG 
CTCCATGTGG CTTCCAAAGG AGGAAATCTT TCCCTAGTGA AAATGCTAAT AGAATCTGGA 
GCAGATGTGA ATCTCAAAGA TAATGCAGGT TGGACACCCC TTCATAAGGC ATCAAGTGGG 
GGATTTGATG ATATAATTAT AGAGTTATTA AAAGCTGGTG CAAATGTGAA CTGTGAAAAT 
GTAGATGGGA TTTTGCCTTT ACATGGTGCT TCTGCAGGGA ACCATTTAAA GGCAGCTGAG 
ATACTCCTAG AACATGGAGC AAACCCTAGT CAGAAAGATC AGAAACAGAG GACTGCTTTG 
GATGAAGCAG ATGATGAAAA AATGAAAGAA CTACTAAAAT CTCATGGTGC TATTGAGACC 
GCTCATGGAG AAGAGAGGAG CTCCATAGTT CCAGTAAAAA TTCCTGCTAT TCGCCCAAAA 
AGATATAAGC CTTTTATCTG TGACAATGAC AAAGCAGTTG GTTCTCCTGC TCCCTCACAT 
AAAGCAAAGA GGAGTGAGAG CCGACCTGTG CATCAGACCA TCAGTGCCAT TCTAAAAGAT 
CTAGAAGAAA AACAGGAAAC CTTACTGAAG TTTGAGATAA GAAACTCTGA AGATGAAGAA 
CAATACATTG AAAAGATGTT AGACATAAAA GAAGTTATGG ATAATATACT TGCTCAACAG 
AAAACAGAAA GGGATGATCT AGCTAAAAAA TACAGGGTAT CCATGGAGTC ATTTAAACAT 
GGGGCCCTGA GAGAACAGCT GGCTAACTTG GCCACAAGAC AAAAGAGTCT TTTAGTTGTA 
GCAAAGAAAC AAAAAAAGAT AAGACTGAAA ATTCAAAACT ATAAGACTGC CACACCATCA 
TCTGGTAATA ATTTAAGGAA GCTGCCATGT AACTCAGACA TCTCCAGTGA CAAGACCAGC 
CAAGATCCTC CTGGAGTGGG AAACTCAGCT CTTGCCCAGT CAGGCTCACT TTCTCCTAAC 
AATCTTGCTT ATAGAAGTAT GCAAGAAATG CCATTTTCTC CAGAAATACA GAGTGACAGC 
CAGAATACTA ACATTTGTTT GAATGCAGAG GCAATAAGAG GAGAAGAATT CCCTGGAAGT 
GACACAAATT CTAAACAAAA TGTCCAGGAT TGTGCCTCAG GTGGGTTATT AAAATCCACC 
TCAGATGACA CTGAGAAAAT GACATCATCA TCCCAACCTG TTGCTCTTAC TTCTCAAGCA 
GAGTATTCAC AAGCAGAGAA TTTTTTTCCA GAAACTTCAG TCAAAGGCTA TGGATTTGAT 
GCTCCTTCAC TAACAGGCAC CATAAATATT TCAGAAGATA AAAGTATATT TTCCCAAAAT 
GATGTCTGTC TACCGGCTTT TCCTCAGAAT NNCAGGGNNG TCNGCCTTTC CCGTTGTAAA 
CCAAAAAGAA GAAACAAGAG AACAGGTTCC CAACAACCAT CTGAGGGAGC ATCTAAGCCT 
CTGGCGCAGA CTGTTGCTAT CTTAGATACC TATACTGCGC GTCAGACAAA GCCGTGTCTT 
AAGAAATCAG CTTCTGTTGC TTTGAATGCA GACAGCATGC AAATCTCTCC TCCCTCTGTG 
TCTGCCCATC AACCTTCTTC TAAAAAGGCT CCACACCACA GCACAACTCC CAGAAAGAAA 
ACTGTGCAGC TGAAAGACTT GATTTTACTT GGAAGAATTA ATCCAGGAAA TAACATTCTG 
GAATTTAAAA CCCAGGAGAC AACCCACAGA GCCAGTGTTT TACTGAGTGG TAAACTGAAG 
GTAGAAAATG GTCAGATTTA CCAGAACCCA GTTACCTGGC TCAAGGATGT CCTGGGAGGT 
GGCAGTTATG TGACCTGGAA TTACGCTTGG AATAAGGTAA CATACCTTGG AAAGGAGCTT 
TTGAAGTATG TTTCTGAAGC ACCTCCTGTT TCTGCAGAAC CGAGTAGCAC ACCTCAGCAG 
CACCAGCCTT GCCTCTCAGC AGGCACTTCC AGAGAGTCGA TGCAAATCAT CCCTCATTAT 
CTACAGATAA AAGAAATACT TCTAATCAGC GATCAAGAGC TTCTGCCGTG CCATGTAATG 
GAGCAGCATT GGAAGTTCTA TGTGGAATGT AAGAAGCTAT CGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446852.1
CDS	114..3641
Translation	MEKGDQASDCDSDETVIEGSVTENELEDDELPWRRFLSQDTTFTSEFCFHSGINGTQNGMHSPEIQLGLKLRKDSQEQNDKNEVLRALSEDLVLQDPQDETTQNQVLLQTRKELPVFSLCQFLTEFQWVTKTTGDTYCSILGPGAEICEHLPHSEKELSENSDSPEVSLLSGTSLMASDLVTLKEQLTEPVKTLAVPNTFSEPGKKVTLTLTSIETKDEESSLETFVSALEKLLESPEYTQEERLLEIMNDFSPQEFLTSLSNSMSSVSVPSSALSAGDRDELGNKAGAVLPAQLLAAINTLPGADVGPICQGQERSSSVSGGNGCLEVQPAMSQKDEDCTHIAQNIEDPKPFRLQTLTHENATSYEQLNKKENSDSMKNTSTQKTPRVLRRSSRLEKLRASRDVHTDVILERPERILSEALSCKDRINSIFTTENFSKRKNMHSSRFKNEQIRKNEQLRIKNEVNTAGINKRNAKGESRLHVASKGGNLSLVKMLIESGADVNLKDNAGWTPLHKASSGGFDDIIIELLKAGANVNCENVDGILPLHGASAGNHLKAAEILLEHGANPSQKDQKQRTALDEADDEKMKELLKSHGAIETAHGEERSSIVPVKIPAIRPKRYKPFICDNDKAVGSPAPSHKAKRSESRPVHQTISAILKDLEEKQETLLKFEIRNSEDEEQYIEKMLDIKEVMDNILAQQKTERDDLAKKYRVSMESFKHGALREQLANLATRQKSLLVVAKKQKKIRLKIQNYKTATPSSGNNLRKLPCNSDISSDKTSQDPPGVGNSALAQSGSLSPNNLAYRSMQEMPFSPEIQSDSQNTNICLNAEAIRGEEFPGSDTNSKQNVQDCASGGLLKSTSDDTEKMTSSSQPVALTSQAEYSQAENFFPETSVKGYGFDAPSLTGTINISEDKSIFSQNDVCLPAFPQNnullRnullVnullLSRCKPKRRNKRTGSQQPSEGASKPLAQTVAILDTYTARQTKPCLKKSASVALNADSMQISPPSVSAHQPSSKKAPHHSTTPRKKTVQLKDLILLGRINPGNNILEFKTQETTHRASVLLSGKLKVENGQIYQNPVTWLKDVLGGGSYVTWNYAWNKVTYLGKELLKYVSEAPPVSAEPSSTPQQHQPCLSAGTSRESMQIIPHYLQIKEILLISDQELLPCHVMEQHWKFYVECKKLSF

Target ORF information:

RefSeq Version	XM_017591363.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat domain 31 (Ankrd31), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591363.1

ORF Insert Sequence:

ATGGAGAAAG GTGACCAGGC TTCCGACTGT GACAGTGATG AAACAGTGAT TGAGGGGTCG 
GTGACAGAGA ACGAACTGGA AGATGATGAG CTTCCCTGGA GAAGGTTCTT GAGTCAAGAT 
ACAACTTTTA CATCTGAGTT CTGTTTTCAT TCTGGTATAA ATGGAACACA GAATGGTATG 
CATTCTCCTG AGATTCAACT TGGACTGAAA CTCAGAAAGG ATTCACAAGA GCAAAATGAT 
AAAAATGAGG TGTTGCGGGC TCTTAGTGAG GACCTAGTGT TACAGGATCC TCAGGATGAA 
ACAACACAAA ATCAAGTTCT GTTACAAACT AGAAAAGAAT TGCCAGTATT TTCTCTGTGT 
CAGTTCCTCA CCGAGTTTCA ATGGGTCACC AAAACTACTG GAGATACATA TTGTTCTATT 
CTAGGGCCTG GAGCTGAAAT CTGTGAACAT TTGCCACACT CAGAAAAGGA ACTAAGTGAA 
AACTCAGATT CCCCTGAAGT TAGTCTTCTC TCAGGGACTT CTCTTATGGC ATCTGATTTG 
GTTACCTTAA AGGAACAATT AACAGAGCCA GTGAAGACCT TAGCAGTACC AAACACATTT 
TCTGAGCCTG GAAAGAAAGT AACACTGACC TTGACTTCTA TAGAAACCAA AGATGAAGAA 
AGCTCTCTTG AAACATTTGT GTCTGCATTA GAAAAGTTAC TAGAATCACC AGAATATACC 
CAAGAAGAAA GACTGCTTGA AATAATGAAT GACTTCAGTC CTCAAGAGTT CTTGACCTCA 
TTGAGCAATT CCATGAGTTC TGTGTCTGTC CCTTCGAGTG CCTTGTCAGC AGGTGATAGA 
GATGAACTAG GAAACAAGGC TGGTGCTGTG TTGCCAGCTC AATTGCTGGC AGCCATCAAC 
ACATTGCCAG GTGCTGATGT GGGACCCATC TGTCAAGGAC AAGAGAGAAG CAGCAGTGTT 
AGTGGTGGAA ATGGATGTTT AGAAGTGCAG CCTGCCATGT CTCAGAAAGA TGAAGATTGT 
ACACACATAG CACAGAATAT AGAAGACCCA AAACCATTTA GATTACAAAC ATTGACTCAT 
GAAAATGCCA CCTCTTATGA GCAACTAAAC AAAAAGGAAA ATTCAGATTC CATGAAAAAC 
ACCTCTACCC AGAAAACACC TCGTGTACTA AGAAGATCAT CTAGACTGGA AAAACTAAGA 
GCAAGCAGAG ATGTGCATAC AGATGTCATA CTGGAGAGGC CAGAGAGGAT TTTATCAGAA 
GCACTTAGCT GTAAGGATCG AATAAATAGC ATATTTACAA CTGAGAATTT CAGTAAAAGG 
AAGAACATGC ATTCATCCAG ATTCAAGAAT GAACAGATAA GGAAAAATGA ACAACTTAGA 
ATAAAAAATG AAGTGAACAC AGCTGGAATC AATAAGAGAA ATGCCAAAGG AGAAAGCCGG 
CTCCATGTGG CTTCCAAAGG AGGAAATCTT TCCCTAGTGA AAATGCTAAT AGAATCTGGA 
GCAGATGTGA ATCTCAAAGA TAATGCAGGT TGGACACCCC TTCATAAGGC ATCAAGTGGG 
GGATTTGATG ATATAATTAT AGAGTTATTA AAAGCTGGTG CAAATGTGAA CTGTGAAAAT 
GTAGATGGGA TTTTGCCTTT ACATGGTGCT TCTGCAGGGA ACCATTTAAA GGCAGCTGAG 
ATACTCCTAG AACATGGAGC AAACCCTAGT CAGAAAGATC AGAAACAGAG GACTGCTTTG 
GATGAAGCAG ATGATGAAAA AATGAAAGAA CTACTAAAAT CTCATGGTGC TATTGAGACC 
GCTCATGGAG AAGAGAGGAG CTCCATAGTT CCAGTAAAAA TTCCTGCTAT TCGCCCAAAA 
AGATATAAGC CTTTTATCTG TGACAATGAC AAAGCAGTTG GTTCTCCTGC TCCCTCACAT 
AAAGCAAAGA GGAGTGAGAG CCGACCTGTG CATCAGACCA TCAGTGCCAT TCTAAAAGAT 
CTAGAAGAAA AACAGGAAAC CTTACTGAAG TTTGAGATAA GAAACTCTGA AGATGAAGAA 
CAATACATTG AAAAGATGTT AGACATAAAA GAAGTTATGG ATAATATACT TGCTCAACAG 
AAAACAGAAA GGGATGATCT AGCTAAAAAA TACAGGGTAT CCATGGAGTC ATTTAAACAT 
GGGGCCCTGA GAGAACAGCT GGCTAACTTG GCCACAAGAC AAAAGAGTCT TTTAGTTGTA 
GCAAAGAAAC AAAAAAAGAT AAGACTGAAA ATTCAAAACT ATAAGACTGC CACACCATCA 
TCTGGTAATA ATTTAAGGAA GCTGCCATGT AACTCAGACA TCTCCAGTGA CAAGACCAGC 
CAAGATCCTC CTGGAGTGGG AAACTCAGCT CTTGCCCAGT CAGGCTCACT TTCTCCTAAC 
AATCTTGCTT ATAGAAGTAT GCAAGAAATG CCATTTTCTC CAGAAATACA GAGTGACAGC 
CAGAATACTA ACATTTGTTT GAATGCAGAG GCAATAAGAG GAGAAGAATT CCCTGGAAGT 
GACACAAATT CTAAACAAAA TGTCCAGGAT TGTGCCTCAG GTGGGTTATT AAAATCCACC 
TCAGATGACA CTGAGAAAAT GACATCATCA TCCCAACCTG TTGCTCTTAC TTCTCAAGCA 
GAGTATTCAC AAGCAGAGAA TTTTTTTCCA GAAACTTCAG TCAAAGGCTA TGGATTTGAT 
GCTCCTTCAC TAACAGGCAC CATAAATATT TCAGAAGATA AAAGTATATT TTCCCAAAAT 
GATGTCTGTC TACCGGCTTT TCCTCAGAAT NNCAGGGNNG TCNGCCTTTC CCGTTGTAAA 
CCAAAAAGAA GAAACAAGAG AACAGGTTCC CAACAACCAT CTGAGGGAGC ATCTAAGCCT 
CTGGCGCAGA CTGTTGCTAT CTTAGATACC TATACTGCGC GTCAGACAAA GCCGTGTCTT 
AAGAAATCAG CTTCTGTTGC TTTGAATGCA GACAGCATGC AAATCTCTCC TCCCTCTGTG 
TCTGCCCATC AACCTTCTTC TAAAAAGGCT CCACACCACA GCACAACTCC CAGAAAGAAA 
ACTGTGCAGC TGAAAGACTT GATTTTACTT GGAAGAATTA ATCCAGGAAA TAACATTCTG 
GAATTTAAAA CCCAGGAGAC AACCCACAGA GCCAGTGTTT TACTGAGTGG TAAACTGAAG 
GTAGAAAATG GTCAGATTTA CCAGAACCCA GTTACCTGGC TCAAGGATGT CCTGGGAGGT 
GGCAGTTATG TGACCTGGAA TTACGCTTGG AATAAGGTAA CATACCTTGG AAAGGAGCTT 
TTGAAGTATG TTTCTGAAGC ACCTCCTGTT TCTGCAGAAC CGAGTAGCAC ACCTCAGCAG 
CACCAGCCTT GCCTCTCAGC AGGCACTTCC AGAGAGTCGA TGCAAATCAT CCCTCATTAT 
CTACAGATAA AAGAAATACT TCTAATCAGC GATCAAGAGC TTCTGCCGTG CCATGTAATG 
GAGCAGCATT GGAAGTTCTA TGTGGAATGT AAGAAGCTAT CGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Ankrd31 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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