PLEKHG4 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLEKHG4
Entrez Gene ID	105305771
Full Name	pleckstrin homology and RhoGEF domain containing G4
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011380573.2	XP_011378875.2	puratrophin-1
XM_011380573.1	XP_011378875.1	puratrophin-1

Pathways from BioSystems
Gene Ontology

The following PLEKHG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPt61503	XM_011380573.1 Latest version!	Pteropus vampyrus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt77165	XM_011380573.2 Latest version!	Pteropus vampyrus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLEKHG4 ( XM_011380573.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011378875.1 Pteropus vampyrus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), mRNA.

Price & Availability↑

CloneID	OPt61503
Clone ID Related Accession (Same CDS sequence)	XM_011380573.1
Accession Version	XM_011380573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	puratrophin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011889175.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAAGGGT CCCCAGACTC CCAGGGCCAT GCTATAGACT GGAGGTTTGC TGTGTGCAGT 
TTCAGAGATG CCTGGGATGA GGAGGTTAAG GACCCCAGTC CTCCAAGACC ATCAGCAGGG 
GCAGCCCAGG GTGACCCGTT ACAGGGCAGC CTCCTGCCTG GGGAGCTTCA ATCACCCCCA 
GGTCAGAAAG CTGCAGATGG GTCGGGGGAT GGCCAGAGGG GCACATTAGG AGGTTCCTCG 
GCCAAATCAG AGGAACCAGT CCCCTCTGGA GAGGAGACCC TCCTATGCCC AGTGTCCTCC 
CATCTCAGCC TGGCACAGGG TGAGAGTGCC AGCCACGGGG GAGGCTTGGC AAGAGACCCA 
GTTCCAGGCA GGGTAGTGTC AGCTGGCTTG GGTTCTGGGG CGTTGGACAG TGACTCTGTG 
GACTTTGGTG ACCCTTTATT TGAGACATCA GAGCTGCTGG AAGCAGACCC CAGTGGATCC 
AGCCTCCCTA AGCCCGCTGA CTACCTCCTG GCCCAAGACC TCACCTGGGA GCTGTTAGCC 
AGTGGCATGG CTGCCCTGCC AGGGACTCGG GATGTAGACG GTCGGGCAGT GCTGCTTCTG 
TGTGCCCATA GCCCGGCTTG GCTTCACCCC AAGTGCAGCA GCCATGAATT GATCCGCCTT 
CTGCTCTACC TGCGAAGCAT CCCCAGGTCT GAAGTACAGG CCTTGGGACT GACTGTGCTA 
GTGGACGCCC GAATTTGTCC CCCAAACTCT TCTCTCTTCT GGGGGCTCAG CCAGTTGCAA 
GAAATAGCCC CAGGATCAGT GCACCAGGTG CTGCTAGTGG GAAAAATGCC TGAGGAGGTA 
CACACAGGGC TGCAGTTAGA ACAGTTGCCC TCTCATCAGA GCATGCTGAC CCACATCTCC 
ACCTCAGGAT TACCCGCTTC GTTGGGAGGA GGCCTGCCTT ACTGCCACCA GGCCTGGTTG 
GACTTCCGGA TGCGTCTGGA AGCCCTGCAA CAGAGCTGCC AGGTGGTTTG TGCCCTGCTC 
CAGGGGGCCA TTGAGAGCAT GAAGGCTGTG CCTCAGCCCA TGGAGTCTGG GGAAGTCAGT 
CGGCTGCTAC GGCAGGCACG GGTTCTTATG CAACATGTGC TAGACTCTCC ACGGCTGGCA 
TGGCTGCAAG GCCAGGGGGC CTTGGAGCTG GCATGGCTGA AACAGAAGGG CCCAGAGGTG 
ACCCAGAGCC CAGATTACAG GCCTGCGGTG GACAAGGTTG ATGAGCTATA TGGCCGTGTG 
GATGGTCTGC TGCACCAACT GACCCTGCAG TGTAACCAGC GAATACAGGC ACTGGAGTTG 
GTCCAGACGC TGGAGGCCCA GGAGGGCGGG CTGCACCAGA TTGAAGTGTG GCTACAGCAG 
GTGGGCTGGC CAGCACTGGA GGAACCAAGG GAGCTCTCAC TGGACATTCT GCTCCAGGCC 
CAAGGCCCTT TTCGGGAGCT GGACCGGATT GCCCAGGAGC AGCTTAGGCA AGGAGAGAAA 
TTTCTGCAGC CCCTGGCTGG CTGGGAGGCA GTTGAACTGG GCCCCCATGG GGCCCGCTTT 
CTGGCCCTGC AAGCCCAGCT GACTAAATTC TCTAGGGCTT TGGCCCAGCG GCGGCAGCGG 
CTGACAGATG CCGAGATGCT GTTGCGGCTC TTCAAGCAGG CCTCGACATG GGCTGAGGAG 
GGGCAGCGGG TTTTGGCAGA GCTGGAGCAG GAGCGCCCAG CAGTCGTGCT GCAACGGCTG 
CAGCTGCACT GGACCAAGCA CCCTGACTTG CCTCCTGCTC ACTTCAGAAA AATGTGGGCT 
TTGGCCACGG GGCTGGGTTC CGAAGGCATC CGCCAGGAGT GCCGCTGTGC CTGGGCGCAA 
TGCCAGGACA CCTGGCTGGC GCTGGACCAG AAGTTAGAGG CTACACTGAA GCCACCACCA 
ACTGGCAGCA CAGCTAGCCT GTGTGTCAGC CAGGTCCCAG CTGCACCTGC CACCCCTCCC 
CTGAGAAAGG CCTACAGCCT TGATCGGAAT CTGGGGCAGA GTCTCAGAGA GCCTGCCCAC 
CATGGCCACC ATGCGGCCAT TATACCTGCC TGCCACGGAC CAGAAGCTGG ATATGGTGCT 
CTGCCAGAGT CCTCCCCAGC CTTGCCTCCA CCAGGCAGCT CTGACCCTAG GAGCCTCAAC 
AGGCTCCAGC TGGTGCTGGC AGAGATGGTG GCAACAGAAC GGGAGTATGT CCGTGCCCTT 
GACTATACCA TGGAGAACTA CTTCCCTGAG CTGGATCGCC CTGATGTGCC CCAGGGCCTC 
CGTGGCCAGC GCGCCCACCT CTTTGGCAAC CTGGAGAAGC TGCGAGACTT CCACTACCAT 
TTCTTCCTGC GTGAGCTGGA GGCTTGCACG CGACACCCAC CTCGGGTGGC CTATGCCTTC 
CTACGCCATA GGGTGCAATT TGGGATGTAT GCGCTGTACA GCAAGAATAA ACCTCGCTCC 
GACGCCCTGA TGACCAGCTA TGGTCACGCC TTCTTCAAGG ACAAACAGCA GGTGCTGGGG 
GACCACCTGG ACTTGGCCTC CTACCTGCTG AAGCCTATCC AGCGCATGAG CAAGTACGCA 
CTGCTGCTGC AGGAGCTGGC GCGGGCCTGC GGGGGGCCCA TGCAGGAGCT GAGTGCCCTG 
CGGGCTGCCC AGAGCCTCGT GCGCTTCCAG CTGCGACACG GCAATGACCT GCTGGCCATG 
GACGCCATCC AGGGCTGTGA CGTTAATCTC AAGGAACAGG GGCAGCTGGT ACGACAAGAT 
GAGTTCACAG TACGCACCGG GCGCCACAAG TCCCTGCGCC GTGTTTTCCT CTTTGAGGAG 
CTGCTGCTTT TCAGCAAGCC TCGCAGAGGA CCCACAGGCA TCGACACATT TGCCTACAAG 
CGCTCCTTCA AGATGGCGGA CCTTGGCCTC ACTGAGTGCT GTGAGGATAA CAACCTGCGC 
TTTGAGATCT GGTTCCGCCG TCGCAAGGCC AGGGATACCT TTGTGCTGCA GGCCGCCAGC 
TTGGCCACCA AGCAGGCTTG GACGGCTGAC ATCTCCCGCC TGCTCTGGAA GCAGGCTGTC 
CACAACAAGG AGGTGCGCAT GGCTGAGATG GTGTCCATGG GTGTGGGGAA CAAAACCTTC 
CGGGACATCG CCCCCAATGA GGAAGCTATC AATGACCGCA CCATCAACTA CGTTTTAAAG 
TGCCGAGATG TTCGCTCTCG GGCCTCCATT GCTGTGGTTC CGTTTGACTA TGACAGCCCC 
TACCTGGGAG CCTCAAGCTC CCTTCCTGCA GACCCTGCCT CTCACTCCGT ACTGGGGTCC 
CTCAACCTGC ACCTGTACAG AGATCCAGCT CTTCTGGGTC TCCGCTGGCC CTTGTATTCT 
ACCAGTTTAC CAGAGGAAGC AGCACTGGAG GCTGAAATGG AGCTGGGCAG CCAGCCATCT 
TTAACTCCTG AGGACTCAGA AGTCTCATCC CAGTGCCCAT CAGCCAGTGG CTCCAGCGGA 
TCTGACAGCA GCTTTGTGTC AGGGCAGGCC CTGGGCAGGG GCCTTGAGGA CTTGTCCTAT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011378875.1
CDS	1..3546
Translation	MEGSPDSQGHAIDWRFAVCSFRDAWDEEVKDPSPPRPSAGAAQGDPLQGSLLPGELQSPPGQKAADGSGDGQRGTLGGSSAKSEEPVPSGEETLLCPVSSHLSLAQGESASHGGGLARDPVPGRVVSAGLGSGALDSDSVDFGDPLFETSELLEADPSGSSLPKPADYLLAQDLTWELLASGMAALPGTRDVDGRAVLLLCAHSPAWLHPKCSSHELIRLLLYLRSIPRSEVQALGLTVLVDARICPPNSSLFWGLSQLQEIAPGSVHQVLLVGKMPEEVHTGLQLEQLPSHQSMLTHISTSGLPASLGGGLPYCHQAWLDFRMRLEALQQSCQVVCALLQGAIESMKAVPQPMESGEVSRLLRQARVLMQHVLDSPRLAWLQGQGALELAWLKQKGPEVTQSPDYRPAVDKVDELYGRVDGLLHQLTLQCNQRIQALELVQTLEAQEGGLHQIEVWLQQVGWPALEEPRELSLDILLQAQGPFRELDRIAQEQLRQGEKFLQPLAGWEAVELGPHGARFLALQAQLTKFSRALAQRRQRLTDAEMLLRLFKQASTWAEEGQRVLAELEQERPAVVLQRLQLHWTKHPDLPPAHFRKMWALATGLGSEGIRQECRCAWAQCQDTWLALDQKLEATLKPPPTGSTASLCVSQVPAAPATPPLRKAYSLDRNLGQSLREPAHHGHHAAIIPACHGPEAGYGALPESSPALPPPGSSDPRSLNRLQLVLAEMVATEREYVRALDYTMENYFPELDRPDVPQGLRGQRAHLFGNLEKLRDFHYHFFLRELEACTRHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMTSYGHAFFKDKQQVLGDHLDLASYLLKPIQRMSKYALLLQELARACGGPMQELSALRAAQSLVRFQLRHGNDLLAMDAIQGCDVNLKEQGQLVRQDEFTVRTGRHKSLRRVFLFEELLLFSKPRRGPTGIDTFAYKRSFKMADLGLTECCEDNNLRFEIWFRRRKARDTFVLQAASLATKQAWTADISRLLWKQAVHNKEVRMAEMVSMGVGNKTFRDIAPNEEAINDRTINYVLKCRDVRSRASIAVVPFDYDSPYLGASSSLPADPASHSVLGSLNLHLYRDPALLGLRWPLYSTSLPEEAALEAEMELGSQPSLTPEDSEVSSQCPSASGSSGSDSSFVSGQALGRGLEDLSYV

Target ORF information:

RefSeq Version	XM_011380573.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011380573.1

ORF Insert Sequence:

ATGGAAGGGT CCCCAGACTC CCAGGGCCAT GCTATAGACT GGAGGTTTGC TGTGTGCAGT 
TTCAGAGATG CCTGGGATGA GGAGGTTAAG GACCCCAGTC CTCCAAGACC ATCAGCAGGG 
GCAGCCCAGG GTGACCCGTT ACAGGGCAGC CTCCTGCCTG GGGAGCTTCA ATCACCCCCA 
GGTCAGAAAG CTGCAGATGG GTCGGGGGAT GGCCAGAGGG GCACATTAGG AGGTTCCTCG 
GCCAAATCAG AGGAACCAGT CCCCTCTGGA GAGGAGACCC TCCTATGCCC AGTGTCCTCC 
CATCTCAGCC TGGCACAGGG TGAGAGTGCC AGCCACGGGG GAGGCTTGGC AAGAGACCCA 
GTTCCAGGCA GGGTAGTGTC AGCTGGCTTG GGTTCTGGGG CGTTGGACAG TGACTCTGTG 
GACTTTGGTG ACCCTTTATT TGAGACATCA GAGCTGCTGG AAGCAGACCC CAGTGGATCC 
AGCCTCCCTA AGCCCGCTGA CTACCTCCTG GCCCAAGACC TCACCTGGGA GCTGTTAGCC 
AGTGGCATGG CTGCCCTGCC AGGGACTCGG GATGTAGACG GTCGGGCAGT GCTGCTTCTG 
TGTGCCCATA GCCCGGCTTG GCTTCACCCC AAGTGCAGCA GCCATGAATT GATCCGCCTT 
CTGCTCTACC TGCGAAGCAT CCCCAGGTCT GAAGTACAGG CCTTGGGACT GACTGTGCTA 
GTGGACGCCC GAATTTGTCC CCCAAACTCT TCTCTCTTCT GGGGGCTCAG CCAGTTGCAA 
GAAATAGCCC CAGGATCAGT GCACCAGGTG CTGCTAGTGG GAAAAATGCC TGAGGAGGTA 
CACACAGGGC TGCAGTTAGA ACAGTTGCCC TCTCATCAGA GCATGCTGAC CCACATCTCC 
ACCTCAGGAT TACCCGCTTC GTTGGGAGGA GGCCTGCCTT ACTGCCACCA GGCCTGGTTG 
GACTTCCGGA TGCGTCTGGA AGCCCTGCAA CAGAGCTGCC AGGTGGTTTG TGCCCTGCTC 
CAGGGGGCCA TTGAGAGCAT GAAGGCTGTG CCTCAGCCCA TGGAGTCTGG GGAAGTCAGT 
CGGCTGCTAC GGCAGGCACG GGTTCTTATG CAACATGTGC TAGACTCTCC ACGGCTGGCA 
TGGCTGCAAG GCCAGGGGGC CTTGGAGCTG GCATGGCTGA AACAGAAGGG CCCAGAGGTG 
ACCCAGAGCC CAGATTACAG GCCTGCGGTG GACAAGGTTG ATGAGCTATA TGGCCGTGTG 
GATGGTCTGC TGCACCAACT GACCCTGCAG TGTAACCAGC GAATACAGGC ACTGGAGTTG 
GTCCAGACGC TGGAGGCCCA GGAGGGCGGG CTGCACCAGA TTGAAGTGTG GCTACAGCAG 
GTGGGCTGGC CAGCACTGGA GGAACCAAGG GAGCTCTCAC TGGACATTCT GCTCCAGGCC 
CAAGGCCCTT TTCGGGAGCT GGACCGGATT GCCCAGGAGC AGCTTAGGCA AGGAGAGAAA 
TTTCTGCAGC CCCTGGCTGG CTGGGAGGCA GTTGAACTGG GCCCCCATGG GGCCCGCTTT 
CTGGCCCTGC AAGCCCAGCT GACTAAATTC TCTAGGGCTT TGGCCCAGCG GCGGCAGCGG 
CTGACAGATG CCGAGATGCT GTTGCGGCTC TTCAAGCAGG CCTCGACATG GGCTGAGGAG 
GGGCAGCGGG TTTTGGCAGA GCTGGAGCAG GAGCGCCCAG CAGTCGTGCT GCAACGGCTG 
CAGCTGCACT GGACCAAGCA CCCTGACTTG CCTCCTGCTC ACTTCAGAAA AATGTGGGCT 
TTGGCCACGG GGCTGGGTTC CGAAGGCATC CGCCAGGAGT GCCGCTGTGC CTGGGCGCAA 
TGCCAGGACA CCTGGCTGGC GCTGGACCAG AAGTTAGAGG CTACACTGAA GCCACCACCA 
ACTGGCAGCA CAGCTAGCCT GTGTGTCAGC CAGGTCCCAG CTGCACCTGC CACCCCTCCC 
CTGAGAAAGG CCTACAGCCT TGATCGGAAT CTGGGGCAGA GTCTCAGAGA GCCTGCCCAC 
CATGGCCACC ATGCGGCCAT TATACCTGCC TGCCACGGAC CAGAAGCTGG ATATGGTGCT 
CTGCCAGAGT CCTCCCCAGC CTTGCCTCCA CCAGGCAGCT CTGACCCTAG GAGCCTCAAC 
AGGCTCCAGC TGGTGCTGGC AGAGATGGTG GCAACAGAAC GGGAGTATGT CCGTGCCCTT 
GACTATACCA TGGAGAACTA CTTCCCTGAG CTGGATCGCC CTGATGTGCC CCAGGGCCTC 
CGTGGCCAGC GCGCCCACCT CTTTGGCAAC CTGGAGAAGC TGCGAGACTT CCACTACCAT 
TTCTTCCTGC GTGAGCTGGA GGCTTGCACG CGACACCCAC CTCGGGTGGC CTATGCCTTC 
CTACGCCATA GGGTGCAATT TGGGATGTAT GCGCTGTACA GCAAGAATAA ACCTCGCTCC 
GACGCCCTGA TGACCAGCTA TGGTCACGCC TTCTTCAAGG ACAAACAGCA GGTGCTGGGG 
GACCACCTGG ACTTGGCCTC CTACCTGCTG AAGCCTATCC AGCGCATGAG CAAGTACGCA 
CTGCTGCTGC AGGAGCTGGC GCGGGCCTGC GGGGGGCCCA TGCAGGAGCT GAGTGCCCTG 
CGGGCTGCCC AGAGCCTCGT GCGCTTCCAG CTGCGACACG GCAATGACCT GCTGGCCATG 
GACGCCATCC AGGGCTGTGA CGTTAATCTC AAGGAACAGG GGCAGCTGGT ACGACAAGAT 
GAGTTCACAG TACGCACCGG GCGCCACAAG TCCCTGCGCC GTGTTTTCCT CTTTGAGGAG 
CTGCTGCTTT TCAGCAAGCC TCGCAGAGGA CCCACAGGCA TCGACACATT TGCCTACAAG 
CGCTCCTTCA AGATGGCGGA CCTTGGCCTC ACTGAGTGCT GTGAGGATAA CAACCTGCGC 
TTTGAGATCT GGTTCCGCCG TCGCAAGGCC AGGGATACCT TTGTGCTGCA GGCCGCCAGC 
TTGGCCACCA AGCAGGCTTG GACGGCTGAC ATCTCCCGCC TGCTCTGGAA GCAGGCTGTC 
CACAACAAGG AGGTGCGCAT GGCTGAGATG GTGTCCATGG GTGTGGGGAA CAAAACCTTC 
CGGGACATCG CCCCCAATGA GGAAGCTATC AATGACCGCA CCATCAACTA CGTTTTAAAG 
TGCCGAGATG TTCGCTCTCG GGCCTCCATT GCTGTGGTTC CGTTTGACTA TGACAGCCCC 
TACCTGGGAG CCTCAAGCTC CCTTCCTGCA GACCCTGCCT CTCACTCCGT ACTGGGGTCC 
CTCAACCTGC ACCTGTACAG AGATCCAGCT CTTCTGGGTC TCCGCTGGCC CTTGTATTCT 
ACCAGTTTAC CAGAGGAAGC AGCACTGGAG GCTGAAATGG AGCTGGGCAG CCAGCCATCT 
TTAACTCCTG AGGACTCAGA AGTCTCATCC CAGTGCCCAT CAGCCAGTGG CTCCAGCGGA 
TCTGACAGCA GCTTTGTGTC AGGGCAGGCC CTGGGCAGGG GCCTTGAGGA CTTGTCCTAT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHG4 ( XM_011380573.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011378875.2 Pteropus vampyrus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), mRNA.

Price & Availability↑

CloneID	OPt77165
Clone ID Related Accession (Same CDS sequence)	XM_011380573.2
Accession Version	XM_011380573.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	puratrophin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011889175.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011380573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG GGTCCCCAGA CTCCCAGGGC CATGCTATAG ACTGGAGGTT TGCTGTGTGC 
AGTTTCAGAG ATGCCTGGGA TGAGGAGGTT AAGGACCCCA GTCCTCCAAG ACCATCAGCA 
GGGGCAGCCC AGGGTGACCC GTTACAGGGC AGCCTCCTGC CTGGGGAGCT TCAATCACCC 
CCAGGTCAGA AAGCTGCAGA TGGGTCGGGG GATGGCCAGA GGGGCACATT AGGAGGTTCC 
TCGGCCAAAT CAGAGGAACC AGTCCCCTCT GGAGAGGAGA CCCTCCTATG CCCAGTGTCC 
TCCCATCTCA GCCTGGCACA GGGTGAGAGT GCCAGCCACG GGGGAGGCTT GGCAAGAGAC 
CCAGTTCCAG GCAGGGTAGT GTCAGCTGGC TTGGGTTCTG GGGCGTTGGA CAGTGACTCT 
GTGGACTTTG GTGACCCTTT ATTTGAGACA TCAGAGCTGC TGGAAGCAGA CCCCAGTGGA 
TCCAGCCTCC CTAAGCCCGC TGACTACCTC CTGGCCCAAG ACCTCACCTG GGAGCTGTTA 
GCCAGTGGCA TGGCTGCCCT GCCAGGGACT CGGGATGTAG ACGGTCGGGC AGTGCTGCTT 
CTGTGTGCCC ATAGCCCGGC TTGGCTTCAC CCCAAGTGCA GCAGCCATGA ATTGATCCGC 
CTTCTGCTCT ACCTGCGAAG CATCCCCAGG TCTGAAGTAC AGGCCTTGGG ACTGACTGTG 
CTAGTGGACG CCCGAATTTG TCCCCCAAAC TCTTCTCTCT TCTGGGGGCT CAGCCAGTTG 
CAAGAAATAG CCCCAGGATC AGTGCACCAG GTGCTGCTAG TGGGAAAAAT GCCTGAGGAG 
GTACACACAG GGCTGCAGTT AGAACAGTTG CCCTCTCATC AGAGCATGCT GACCCACATC 
TCCACCTCAG GATTACCCGC TTCGTTGGGA GGAGGCCTGC CTTACTGCCA CCAGGCCTGG 
TTGGACTTCC GGATGCGTCT GGAAGCCCTG CAACAGAGCT GCCAGGTGGT TTGTGCCCTG 
CTCCAGGGGG CCATTGAGAG CATGAAGGCT GTGCCTCAGC CCATGGAGTC TGGGGAAGTC 
AGTCGGCTGC TACGGCAGGC ACGGGTTCTT ATGCAACATG TGCTAGACTC TCCACGGCTG 
GCATGGCTGC AAGGCCAGGG GGCCTTGGAG CTGGCATGGC TGAAACAGAA GGGCCCAGAG 
GTGACCCAGA GCCCAGATTA CAGGCCTGCG GTGGACAAGG TTGATGAGCT ATATGGCCGT 
GTGGATGGTC TGCTGCACCA ACTGACCCTG CAGTGTAACC AGCGAATACA GGCACTGGAG 
TTGGTCCAGA CGCTGGAGGC CCAGGAGGGC GGGCTGCACC AGATTGAAGT GTGGCTACAG 
CAGGTGGGCT GGCCAGCACT GGAGGAACCA AGGGAGCTCT CACTGGACAT TCTGCTCCAG 
GCCCAAGGCC CTTTTCGGGA GCTGGACCGG ATTGCCCAGG AGCAGCTTAG GCAAGGAGAG 
AAATTTCTGC AGCCCCTGGC TGGCTGGGAG GCAGTTGAAC TGGGCCCCCA TGGGGCCCGC 
TTTCTGGCCC TGCAAGCCCA GCTGACTAAA TTCTCTAGGG CTTTGGCCCA GCGGCGGCAG 
CGGCTGACAG ATGCCGAGAT GCTGTTGCGG CTCTTCAAGC AGGCCTCGAC ATGGGCTGAG 
GAGGGGCAGC GGGTTTTGGC AGAGCTGGAG CAGGAGCGCC CAGCAGTCGT GCTGCAACGG 
CTGCAGCTGC ACTGGACCAA GCACCCTGAC TTGCCTCCTG CTCACTTCAG AAAAATGTGG 
GCTTTGGCCA CGGGGCTGGG TTCCGAAGGC ATCCGCCAGG AGTGCCGCTG TGCCTGGGCG 
CAATGCCAGG ACACCTGGCT GGCGCTGGAC CAGAAGTTAG AGGCTACACT GAAGCCACCA 
CCAACTGGCA GCACAGCTAG CCTGTGTGTC AGCCAGGTCC CAGCTGCACC TGCCACCCCT 
CCCCTGAGAA AGGCCTACAG CCTTGATCGG AATCTGGGGC AGAGTCTCAG AGAGCCTGCC 
CACCATGGCC ACCATGCGGC CATTATACCT GCCTGCCACG GACCAGAAGC TGGATATGGT 
GCTCTGCCAG AGTCCTCCCC AGCCTTGCCT CCACCAGGCA GCTCTGACCC TAGGAGCCTC 
AACAGGCTCC AGCTGGTGCT GGCAGAGATG GTGGCAACAG AACGGGAGTA TGTCCGTGCC 
CTTGACTATA CCATGGAGAA CTACTTCCCT GAGCTGGATC GCCCTGATGT GCCCCAGGGC 
CTCCGTGGCC AGCGCGCCCA CCTCTTTGGC AACCTGGAGA AGCTGCGAGA CTTCCACTAC 
CATTTCTTCC TGCGTGAGCT GGAGGCTTGC ACGCGACACC CACCTCGGGT GGCCTATGCC 
TTCCTACGCC ATAGGGTGCA ATTTGGGATG TATGCGCTGT ACAGCAAGAA TAAACCTCGC 
TCCGACGCCC TGATGACCAG CTATGGTCAC GCCTTCTTCA AGGACAAACA GCAGGTGCTG 
GGGGACCACC TGGACTTGGC CTCCTACCTG CTGAAGCCTA TCCAGCGCAT GAGCAAGTAC 
GCACTGCTGC TGCAGGAGCT GGCGCGGGCC TGCGGGGGGC CCATGCAGGA GCTGAGTGCC 
CTGCGGGCTG CCCAGAGCCT CGTGCGCTTC CAGCTGCGAC ACGGCAATGA CCTGCTGGCC 
ATGGACGCCA TCCAGGGCTG TGACGTTAAT CTCAAGGAAC AGGGGCAGCT GGTACGACAA 
GATGAGTTCA CAGTACGCAC CGGGCGCCAC AAGTCCCTGC GCCGTGTTTT CCTCTTTGAG 
GAGCTGCTGC TTTTCAGCAA GCCTCGCAGA GGACCCACAG GCATCGACAC ATTTGCCTAC 
AAGCGCTCCT TCAAGATGGC GGACCTTGGC CTCACTGAGT GCTGTGAGGA TAACAACCTG 
CGCTTTGAGA TCTGGTTCCG CCGTCGCAAG GCCAGGGATA CCTTTGTGCT GCAGGCCGCC 
AGCTTGGCCA CCAAGCAGGC TTGGACGGCT GACATCTCCC GCCTGCTCTG GAAGCAGGCT 
GTCCACAACA AGGAGGTGCG CATGGCTGAG ATGGTGTCCA TGGGTGTGGG GAACAAAACC 
TTCCGGGACA TCGCCCCCAA TGAGGAAGCT ATCAATGACC GCACCATCAA CTACGTTTTA 
AAGTGCCGAG ATGTTCGCTC TCGGGCCTCC ATTGCTGTGG TTCCGTTTGA CTATGACAGC 
CCCTACCTGG GAGCCTCAAG CTCCCTTCCT GCAGACCCTG CCTCTCACTC CGTACTGGGG 
TCCCTCAACC TGCACCTGTA CAGAGATCCA GCTCTTCTGG GTCTCCGCTG GCCCTTGTAT 
TCTACCAGTT TACCAGAGGA AGCAGCACTG GAGGCTGAAA TGGAGCTGGG CAGCCAGCCA 
TCTTTAACTC CTGAGGACTC AGAAGTCTCA TCCCAGTGCC CATCAGCCAG TGGCTCCAGC 
GGATCTGACA GCAGCTTTGT GTCAGGGCAG GCCCTGGGCA GGGGCCTTGA GGACTTGTCC 
TATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011378875.2
CDS	472..4020
Translation	MMEGSPDSQGHAIDWRFAVCSFRDAWDEEVKDPSPPRPSAGAAQGDPLQGSLLPGELQSPPGQKAADGSGDGQRGTLGGSSAKSEEPVPSGEETLLCPVSSHLSLAQGESASHGGGLARDPVPGRVVSAGLGSGALDSDSVDFGDPLFETSELLEADPSGSSLPKPADYLLAQDLTWELLASGMAALPGTRDVDGRAVLLLCAHSPAWLHPKCSSHELIRLLLYLRSIPRSEVQALGLTVLVDARICPPNSSLFWGLSQLQEIAPGSVHQVLLVGKMPEEVHTGLQLEQLPSHQSMLTHISTSGLPASLGGGLPYCHQAWLDFRMRLEALQQSCQVVCALLQGAIESMKAVPQPMESGEVSRLLRQARVLMQHVLDSPRLAWLQGQGALELAWLKQKGPEVTQSPDYRPAVDKVDELYGRVDGLLHQLTLQCNQRIQALELVQTLEAQEGGLHQIEVWLQQVGWPALEEPRELSLDILLQAQGPFRELDRIAQEQLRQGEKFLQPLAGWEAVELGPHGARFLALQAQLTKFSRALAQRRQRLTDAEMLLRLFKQASTWAEEGQRVLAELEQERPAVVLQRLQLHWTKHPDLPPAHFRKMWALATGLGSEGIRQECRCAWAQCQDTWLALDQKLEATLKPPPTGSTASLCVSQVPAAPATPPLRKAYSLDRNLGQSLREPAHHGHHAAIIPACHGPEAGYGALPESSPALPPPGSSDPRSLNRLQLVLAEMVATEREYVRALDYTMENYFPELDRPDVPQGLRGQRAHLFGNLEKLRDFHYHFFLRELEACTRHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMTSYGHAFFKDKQQVLGDHLDLASYLLKPIQRMSKYALLLQELARACGGPMQELSALRAAQSLVRFQLRHGNDLLAMDAIQGCDVNLKEQGQLVRQDEFTVRTGRHKSLRRVFLFEELLLFSKPRRGPTGIDTFAYKRSFKMADLGLTECCEDNNLRFEIWFRRRKARDTFVLQAASLATKQAWTADISRLLWKQAVHNKEVRMAEMVSMGVGNKTFRDIAPNEEAINDRTINYVLKCRDVRSRASIAVVPFDYDSPYLGASSSLPADPASHSVLGSLNLHLYRDPALLGLRWPLYSTSLPEEAALEAEMELGSQPSLTPEDSEVSSQCPSASGSSGSDSSFVSGQALGRGLEDLSYV

Target ORF information:

RefSeq Version	XM_011380573.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011380573.2

ORF Insert Sequence:

ATGATGGAAG GGTCCCCAGA CTCCCAGGGC CATGCTATAG ACTGGAGGTT TGCTGTGTGC 
AGTTTCAGAG ATGCCTGGGA TGAGGAGGTT AAGGACCCCA GTCCTCCAAG ACCATCAGCA 
GGGGCAGCCC AGGGTGACCC GTTACAGGGC AGCCTCCTGC CTGGGGAGCT TCAATCACCC 
CCAGGTCAGA AAGCTGCAGA TGGGTCGGGG GATGGCCAGA GGGGCACATT AGGAGGTTCC 
TCGGCCAAAT CAGAGGAACC AGTCCCCTCT GGAGAGGAGA CCCTCCTATG CCCAGTGTCC 
TCCCATCTCA GCCTGGCACA GGGTGAGAGT GCCAGCCACG GGGGAGGCTT GGCAAGAGAC 
CCAGTTCCAG GCAGGGTAGT GTCAGCTGGC TTGGGTTCTG GGGCGTTGGA CAGTGACTCT 
GTGGACTTTG GTGACCCTTT ATTTGAGACA TCAGAGCTGC TGGAAGCAGA CCCCAGTGGA 
TCCAGCCTCC CTAAGCCCGC TGACTACCTC CTGGCCCAAG ACCTCACCTG GGAGCTGTTA 
GCCAGTGGCA TGGCTGCCCT GCCAGGGACT CGGGATGTAG ACGGTCGGGC AGTGCTGCTT 
CTGTGTGCCC ATAGCCCGGC TTGGCTTCAC CCCAAGTGCA GCAGCCATGA ATTGATCCGC 
CTTCTGCTCT ACCTGCGAAG CATCCCCAGG TCTGAAGTAC AGGCCTTGGG ACTGACTGTG 
CTAGTGGACG CCCGAATTTG TCCCCCAAAC TCTTCTCTCT TCTGGGGGCT CAGCCAGTTG 
CAAGAAATAG CCCCAGGATC AGTGCACCAG GTGCTGCTAG TGGGAAAAAT GCCTGAGGAG 
GTACACACAG GGCTGCAGTT AGAACAGTTG CCCTCTCATC AGAGCATGCT GACCCACATC 
TCCACCTCAG GATTACCCGC TTCGTTGGGA GGAGGCCTGC CTTACTGCCA CCAGGCCTGG 
TTGGACTTCC GGATGCGTCT GGAAGCCCTG CAACAGAGCT GCCAGGTGGT TTGTGCCCTG 
CTCCAGGGGG CCATTGAGAG CATGAAGGCT GTGCCTCAGC CCATGGAGTC TGGGGAAGTC 
AGTCGGCTGC TACGGCAGGC ACGGGTTCTT ATGCAACATG TGCTAGACTC TCCACGGCTG 
GCATGGCTGC AAGGCCAGGG GGCCTTGGAG CTGGCATGGC TGAAACAGAA GGGCCCAGAG 
GTGACCCAGA GCCCAGATTA CAGGCCTGCG GTGGACAAGG TTGATGAGCT ATATGGCCGT 
GTGGATGGTC TGCTGCACCA ACTGACCCTG CAGTGTAACC AGCGAATACA GGCACTGGAG 
TTGGTCCAGA CGCTGGAGGC CCAGGAGGGC GGGCTGCACC AGATTGAAGT GTGGCTACAG 
CAGGTGGGCT GGCCAGCACT GGAGGAACCA AGGGAGCTCT CACTGGACAT TCTGCTCCAG 
GCCCAAGGCC CTTTTCGGGA GCTGGACCGG ATTGCCCAGG AGCAGCTTAG GCAAGGAGAG 
AAATTTCTGC AGCCCCTGGC TGGCTGGGAG GCAGTTGAAC TGGGCCCCCA TGGGGCCCGC 
TTTCTGGCCC TGCAAGCCCA GCTGACTAAA TTCTCTAGGG CTTTGGCCCA GCGGCGGCAG 
CGGCTGACAG ATGCCGAGAT GCTGTTGCGG CTCTTCAAGC AGGCCTCGAC ATGGGCTGAG 
GAGGGGCAGC GGGTTTTGGC AGAGCTGGAG CAGGAGCGCC CAGCAGTCGT GCTGCAACGG 
CTGCAGCTGC ACTGGACCAA GCACCCTGAC TTGCCTCCTG CTCACTTCAG AAAAATGTGG 
GCTTTGGCCA CGGGGCTGGG TTCCGAAGGC ATCCGCCAGG AGTGCCGCTG TGCCTGGGCG 
CAATGCCAGG ACACCTGGCT GGCGCTGGAC CAGAAGTTAG AGGCTACACT GAAGCCACCA 
CCAACTGGCA GCACAGCTAG CCTGTGTGTC AGCCAGGTCC CAGCTGCACC TGCCACCCCT 
CCCCTGAGAA AGGCCTACAG CCTTGATCGG AATCTGGGGC AGAGTCTCAG AGAGCCTGCC 
CACCATGGCC ACCATGCGGC CATTATACCT GCCTGCCACG GACCAGAAGC TGGATATGGT 
GCTCTGCCAG AGTCCTCCCC AGCCTTGCCT CCACCAGGCA GCTCTGACCC TAGGAGCCTC 
AACAGGCTCC AGCTGGTGCT GGCAGAGATG GTGGCAACAG AACGGGAGTA TGTCCGTGCC 
CTTGACTATA CCATGGAGAA CTACTTCCCT GAGCTGGATC GCCCTGATGT GCCCCAGGGC 
CTCCGTGGCC AGCGCGCCCA CCTCTTTGGC AACCTGGAGA AGCTGCGAGA CTTCCACTAC 
CATTTCTTCC TGCGTGAGCT GGAGGCTTGC ACGCGACACC CACCTCGGGT GGCCTATGCC 
TTCCTACGCC ATAGGGTGCA ATTTGGGATG TATGCGCTGT ACAGCAAGAA TAAACCTCGC 
TCCGACGCCC TGATGACCAG CTATGGTCAC GCCTTCTTCA AGGACAAACA GCAGGTGCTG 
GGGGACCACC TGGACTTGGC CTCCTACCTG CTGAAGCCTA TCCAGCGCAT GAGCAAGTAC 
GCACTGCTGC TGCAGGAGCT GGCGCGGGCC TGCGGGGGGC CCATGCAGGA GCTGAGTGCC 
CTGCGGGCTG CCCAGAGCCT CGTGCGCTTC CAGCTGCGAC ACGGCAATGA CCTGCTGGCC 
ATGGACGCCA TCCAGGGCTG TGACGTTAAT CTCAAGGAAC AGGGGCAGCT GGTACGACAA 
GATGAGTTCA CAGTACGCAC CGGGCGCCAC AAGTCCCTGC GCCGTGTTTT CCTCTTTGAG 
GAGCTGCTGC TTTTCAGCAA GCCTCGCAGA GGACCCACAG GCATCGACAC ATTTGCCTAC 
AAGCGCTCCT TCAAGATGGC GGACCTTGGC CTCACTGAGT GCTGTGAGGA TAACAACCTG 
CGCTTTGAGA TCTGGTTCCG CCGTCGCAAG GCCAGGGATA CCTTTGTGCT GCAGGCCGCC 
AGCTTGGCCA CCAAGCAGGC TTGGACGGCT GACATCTCCC GCCTGCTCTG GAAGCAGGCT 
GTCCACAACA AGGAGGTGCG CATGGCTGAG ATGGTGTCCA TGGGTGTGGG GAACAAAACC 
TTCCGGGACA TCGCCCCCAA TGAGGAAGCT ATCAATGACC GCACCATCAA CTACGTTTTA 
AAGTGCCGAG ATGTTCGCTC TCGGGCCTCC ATTGCTGTGG TTCCGTTTGA CTATGACAGC 
CCCTACCTGG GAGCCTCAAG CTCCCTTCCT GCAGACCCTG CCTCTCACTC CGTACTGGGG 
TCCCTCAACC TGCACCTGTA CAGAGATCCA GCTCTTCTGG GTCTCCGCTG GCCCTTGTAT 
TCTACCAGTT TACCAGAGGA AGCAGCACTG GAGGCTGAAA TGGAGCTGGG CAGCCAGCCA 
TCTTTAACTC CTGAGGACTC AGAAGTCTCA TCCCAGTGCC CATCAGCCAG TGGCTCCAGC 
GGATCTGACA GCAGCTTTGT GTCAGGGCAG GCCCTGGGCA GGGGCCTTGA GGACTTGTCC 
TATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PLEKHG4 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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