KIF27 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	KIF27
Entrez Gene ID	105300034
Full Name	kinesin family member 27
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023537757.1	XP_023393525.1	kinesin-like protein KIF27 isoform X3
XM_023537760.1	XP_023393528.1	kinesin-like protein KIF27 isoform X5
XM_023537758.1	XP_023393526.1	kinesin-like protein KIF27 isoform X4
XM_011372048.2	XP_011370350.1	kinesin-like protein KIF27 isoform X1
XM_011372048.1	XP_011370350.1	kinesin-like protein KIF27
XM_023537756.1	XP_023393524.1	kinesin-like protein KIF27 isoform X2

Pathways from BioSystems
Gene Ontology

The following KIF27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIF27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt73952	XM_023537760.1 Latest version!	Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPt55372	XM_011372048.2 Latest version!	Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPt73949	XM_023537756.1 Latest version!	Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPt73950	XM_023537757.1 Latest version!	Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPt73951	XM_023537758.1 Latest version!	Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OPt55372	XM_011372048.1	Pteropus vampyrus kinesin family member 27 (KIF27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIF27 ( XM_023537760.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023393528.1 Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPt73952
Clone ID Related Accession (Same CDS sequence)	XM_023537760.1
Accession Version	XM_023537760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	kinesin-like protein KIF27 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888980.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAACACAGTG TGCTCTCGCT GCTGATGAAG TAGTATTTAA TCAGAAGGAA 
CTGGAGGTGA AGGAACTAAA GAATCAAGTG CAAATGATGG TACAGGAAAA CAAAGGACAT 
GTTATATCTT TGAAAGAAGC GCAAAAAGTT AATAGATTAC AGAATGAAAA AATAATAGAA 
CAACAACTTC TTATGGATCA GCTGAATGAA GAACTAACAA AACTTAACCT GTCAATGACT 
TCTTCAGTTA AGGAAACTTG TGGAGATGGG CCAGATGCCA GGATACCTGA AAAGAGACCA 
TATACTGTAC CATTTGATAC TCACCTGGGG CATTATATTT ATGTCCCAGC AAGATCAGAT 
GCCAGGAAGG TCTACACAAG TCCTGCTGCG TACTCTCTGG ATCGCATAGT TGCTGGATTC 
CGAACACGAA GTCAGATGCT GCTGGGTCAT ATAGAAGACC AAGATGAAGT CATCCACTGC 
CAGTTTTCTG ATAACAGTGA TGAGGAAGAA TCAGAGGGCC AAGAGAAATC TGATATTAGA 
ACTAGAAGTC ACTCATGGAT TCAAAAGTCT GGCTCCCTTT GTTCTCTTGT TGAACTGAAT 
GATATTCATG ATCAAAAACA AAAGTCAAGT TTGGGGAATG AAGATTTAAA GATTGAATGT 
CTCCAGGAGA GTCAAGAGTT GAATTTGCAA AAATTAAGGA ATTCAGAACT CATACTTAAT 
GAAGCAAAAC AAAAAATGAG AGAACTTACA ATTAACATCA AGATGAAAGA AGATCTAATT 
AAAGAATTAA TGAAAACAGG TAATGATGCC AAGTCTGTAA GCAAACAGTA TTCTCTGAAA 
GTAACAAAAC TTGCACATGA AGCAGAACAG GCAAAAGTGG AATTAATTGA AACAGAAAAA 
CAACTACAGG AACTGGAAAA TAAAGATCTT TCTGATGTTG CTTTGAAAGT AAAATTACAG 
AGAGAGTTCC GTAAAAAGAT GGATGCCGCA AAGCTAAAAG TCCAGGTCTT ACAGAAGAAG 
CAACAAAATA GTAAGAAATT GGCATCGCTT TCGATCCAAA ATGAGAAACG TGCTAATGAA 
CTAGAGCAGA GTGTAGATCA CATGAAATAT CAAAAGGTAC AGCTGCAAAA AAGACTTCGA 
GAAGAAAATG AAAAAAGGAA GCAACTGGAT ACAGAAATTA AGCGGGATCA ACAAAAAATC 
AAAGAACTAA AGTTAAAAAC AGAACCAGAA GAAGGTTTAA AACTGAAAGC TGAGGACCTT 
GATGCATTGA AGATGAAAAG GAGAAAAGGT TCGTTTGGAA GTATAGACCA ACTCCAGAAA 
TTGGATGAGC AAAAGAAATG GTTAGATGAA GAAGTAGAAA AAGTTCTGAA CCAACGCCAA 
GAATTAGAGG AGCTGGAAGA AGACTTAAAG AAACGGGAGG CTATAGTTTC TAAAAAGGAG 
GCCCTGTTAC AGGAGAAGAG CCACCTGGAG AATAAGAAGT TGAGATCTAG TCAGGCCTTA 
AACACAGATA GTTTGAAAAT ATCAACTCGC CTGAACTTGC TGGACCAAGA GTTGTCTGAA 
AAGAATGTAC AGCTCCAGAG CAGCACAGCT GAGGAGAAAA TAAAGATTTC AGAACAAGTC 
CAAGCCCTCC AGAAAGAAAA AGATCAGCTA CAGAGAAGAA GAAACAGTGT GGATGAGAAA 
CTTAAAAATG GTAGCGTGTT ATCACCTGAA GAAGAACATA TTCTTTTCCA ACTTGAAGAA 
GGGATTGAAG CTTTGGAAGC TGCAATTGAA TACAAGAATG AAAGTATCCA GAGTCGCCAG 
AACTCGCTCA GAGCTTCATT CCAAAACCTC TCTCATAGTG AAGCAAATGT CTTGGAAAAA 
CTCATTGGCC TGAATCCTAT TGAGATTAGA GCTATTCTTT TCAGATACTT CAATAAGGTG 
GTTAATTTGC GAGAAGCTGA ACGGAAACTA CAGTTACAGA ATAAAGAAAT TAAAATGAAG 
ATTCTGGAAC GGGATAATGT GGTTCGTGAG TTAGAATCTG CACTGGAGCA TCTGAAATTG 
CAATGTGACC GAAGACTGAC CCTCCAGCAA AAGGAACATG AGCAAAAAAT GCAGTTACTA 
TTACATCATT TCCAAGAGCA AGATGGGGAA GGCATCGTGG AAACTTTAAA GACATATGAA 
GATAAAATCC AACAGCTAGA AAAAGATCTT TATTTCTATA AGAAAACTAG CAGAGATCTT 
AAGAAGAAAC TTAAGGAACT GTTAGGGGAA GCAATTCGAA GGCAACTAGT ACCATCTGAA 
CATCATTATG TTGGAGATGG AGTCCTGAAC CCAGAAGAAG CAGGCACGGT TTCAGAAGAA 
TTAAAATGGG CATCTAAAAC TGAAAGTATG AAGTTAAGTG GAAAAGAAAG AGAATTAAAC 
AGTTCAGCAA GTAGCTTAAG AACACAACGA AATCCTCAGA AGCTCTGGGA AGATGGCCCA 
GAATTACCTC CAATTTATAG CTCTTTAGCA CCCACTAGTG GGCATTTGTT AAGCAATGAG 
GATAAAACAG AAGCAGATGA AAATCAGATT ACAAAAACTC ACAGTTGCAC ATCACCCCAA 
ATTCAGCCAG TGGGAAATGT GGGACAGATT CATGGTGTCA CACCTGTAAA GTTGTGTCGC 
AAAGAGTTAC GTCAGATTTC TGCCGTGGAA CTCTCATTAC GACGTTCCAG TCTTGGAGTT 
GGGGTTGGAT CTATGGCTGC TGATTCCATT GAAGTAGCTA GAAAACAGAG TGACTTAAAA 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023393528.1
CDS	141..2906
Translation	MKKETQCALAADEVVFNQKELEVKELKNQVQMMVQENKGHVISLKEAQKVNRLQNEKIIEQQLLMDQLNEELTKLNLSMTSSVKETCGDGPDARIPEKRPYTVPFDTHLGHYIYVPARSDARKVYTSPAAYSLDRIVAGFRTRSQMLLGHIEDQDEVIHCQFSDNSDEEESEGQEKSDIRTRSHSWIQKSGSLCSLVELNDIHDQKQKSSLGNEDLKIECLQESQELNLQKLRNSELILNEAKQKMRELTINIKMKEDLIKELMKTGNDAKSVSKQYSLKVTKLAHEAEQAKVELIETEKQLQELENKDLSDVALKVKLQREFRKKMDAAKLKVQVLQKKQQNSKKLASLSIQNEKRANELEQSVDHMKYQKVQLQKRLREENEKRKQLDTEIKRDQQKIKELKLKTEPEEGLKLKAEDLDALKMKRRKGSFGSIDQLQKLDEQKKWLDEEVEKVLNQRQELEELEEDLKKREAIVSKKEALLQEKSHLENKKLRSSQALNTDSLKISTRLNLLDQELSEKNVQLQSSTAEEKIKISEQVQALQKEKDQLQRRRNSVDEKLKNGSVLSPEEEHILFQLEEGIEALEAAIEYKNESIQSRQNSLRASFQNLSHSEANVLEKLIGLNPIEIRAILFRYFNKVVNLREAERKLQLQNKEIKMKILERDNVVRELESALEHLKLQCDRRLTLQQKEHEQKMQLLLHHFQEQDGEGIVETLKTYEDKIQQLEKDLYFYKKTSRDLKKKLKELLGEAIRRQLVPSEHHYVGDGVLNPEEAGTVSEELKWASKTESMKLSGKERELNSSASSLRTQRNPQKLWEDGPELPPIYSSLAPTSGHLLSNEDKTEADENQITKTHSCTSPQIQPVGNVGQIHGVTPVKLCRKELRQISAVELSLRRSSLGVGVGSMAADSIEVARKQSDLKT

Target ORF information:

RefSeq Version	XM_023537760.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023537760.1

ORF Insert Sequence:

ATGAAAAAGG AAACACAGTG TGCTCTCGCT GCTGATGAAG TAGTATTTAA TCAGAAGGAA 
CTGGAGGTGA AGGAACTAAA GAATCAAGTG CAAATGATGG TACAGGAAAA CAAAGGACAT 
GTTATATCTT TGAAAGAAGC GCAAAAAGTT AATAGATTAC AGAATGAAAA AATAATAGAA 
CAACAACTTC TTATGGATCA GCTGAATGAA GAACTAACAA AACTTAACCT GTCAATGACT 
TCTTCAGTTA AGGAAACTTG TGGAGATGGG CCAGATGCCA GGATACCTGA AAAGAGACCA 
TATACTGTAC CATTTGATAC TCACCTGGGG CATTATATTT ATGTCCCAGC AAGATCAGAT 
GCCAGGAAGG TCTACACAAG TCCTGCTGCG TACTCTCTGG ATCGCATAGT TGCTGGATTC 
CGAACACGAA GTCAGATGCT GCTGGGTCAT ATAGAAGACC AAGATGAAGT CATCCACTGC 
CAGTTTTCTG ATAACAGTGA TGAGGAAGAA TCAGAGGGCC AAGAGAAATC TGATATTAGA 
ACTAGAAGTC ACTCATGGAT TCAAAAGTCT GGCTCCCTTT GTTCTCTTGT TGAACTGAAT 
GATATTCATG ATCAAAAACA AAAGTCAAGT TTGGGGAATG AAGATTTAAA GATTGAATGT 
CTCCAGGAGA GTCAAGAGTT GAATTTGCAA AAATTAAGGA ATTCAGAACT CATACTTAAT 
GAAGCAAAAC AAAAAATGAG AGAACTTACA ATTAACATCA AGATGAAAGA AGATCTAATT 
AAAGAATTAA TGAAAACAGG TAATGATGCC AAGTCTGTAA GCAAACAGTA TTCTCTGAAA 
GTAACAAAAC TTGCACATGA AGCAGAACAG GCAAAAGTGG AATTAATTGA AACAGAAAAA 
CAACTACAGG AACTGGAAAA TAAAGATCTT TCTGATGTTG CTTTGAAAGT AAAATTACAG 
AGAGAGTTCC GTAAAAAGAT GGATGCCGCA AAGCTAAAAG TCCAGGTCTT ACAGAAGAAG 
CAACAAAATA GTAAGAAATT GGCATCGCTT TCGATCCAAA ATGAGAAACG TGCTAATGAA 
CTAGAGCAGA GTGTAGATCA CATGAAATAT CAAAAGGTAC AGCTGCAAAA AAGACTTCGA 
GAAGAAAATG AAAAAAGGAA GCAACTGGAT ACAGAAATTA AGCGGGATCA ACAAAAAATC 
AAAGAACTAA AGTTAAAAAC AGAACCAGAA GAAGGTTTAA AACTGAAAGC TGAGGACCTT 
GATGCATTGA AGATGAAAAG GAGAAAAGGT TCGTTTGGAA GTATAGACCA ACTCCAGAAA 
TTGGATGAGC AAAAGAAATG GTTAGATGAA GAAGTAGAAA AAGTTCTGAA CCAACGCCAA 
GAATTAGAGG AGCTGGAAGA AGACTTAAAG AAACGGGAGG CTATAGTTTC TAAAAAGGAG 
GCCCTGTTAC AGGAGAAGAG CCACCTGGAG AATAAGAAGT TGAGATCTAG TCAGGCCTTA 
AACACAGATA GTTTGAAAAT ATCAACTCGC CTGAACTTGC TGGACCAAGA GTTGTCTGAA 
AAGAATGTAC AGCTCCAGAG CAGCACAGCT GAGGAGAAAA TAAAGATTTC AGAACAAGTC 
CAAGCCCTCC AGAAAGAAAA AGATCAGCTA CAGAGAAGAA GAAACAGTGT GGATGAGAAA 
CTTAAAAATG GTAGCGTGTT ATCACCTGAA GAAGAACATA TTCTTTTCCA ACTTGAAGAA 
GGGATTGAAG CTTTGGAAGC TGCAATTGAA TACAAGAATG AAAGTATCCA GAGTCGCCAG 
AACTCGCTCA GAGCTTCATT CCAAAACCTC TCTCATAGTG AAGCAAATGT CTTGGAAAAA 
CTCATTGGCC TGAATCCTAT TGAGATTAGA GCTATTCTTT TCAGATACTT CAATAAGGTG 
GTTAATTTGC GAGAAGCTGA ACGGAAACTA CAGTTACAGA ATAAAGAAAT TAAAATGAAG 
ATTCTGGAAC GGGATAATGT GGTTCGTGAG TTAGAATCTG CACTGGAGCA TCTGAAATTG 
CAATGTGACC GAAGACTGAC CCTCCAGCAA AAGGAACATG AGCAAAAAAT GCAGTTACTA 
TTACATCATT TCCAAGAGCA AGATGGGGAA GGCATCGTGG AAACTTTAAA GACATATGAA 
GATAAAATCC AACAGCTAGA AAAAGATCTT TATTTCTATA AGAAAACTAG CAGAGATCTT 
AAGAAGAAAC TTAAGGAACT GTTAGGGGAA GCAATTCGAA GGCAACTAGT ACCATCTGAA 
CATCATTATG TTGGAGATGG AGTCCTGAAC CCAGAAGAAG CAGGCACGGT TTCAGAAGAA 
TTAAAATGGG CATCTAAAAC TGAAAGTATG AAGTTAAGTG GAAAAGAAAG AGAATTAAAC 
AGTTCAGCAA GTAGCTTAAG AACACAACGA AATCCTCAGA AGCTCTGGGA AGATGGCCCA 
GAATTACCTC CAATTTATAG CTCTTTAGCA CCCACTAGTG GGCATTTGTT AAGCAATGAG 
GATAAAACAG AAGCAGATGA AAATCAGATT ACAAAAACTC ACAGTTGCAC ATCACCCCAA 
ATTCAGCCAG TGGGAAATGT GGGACAGATT CATGGTGTCA CACCTGTAAA GTTGTGTCGC 
AAAGAGTTAC GTCAGATTTC TGCCGTGGAA CTCTCATTAC GACGTTCCAG TCTTGGAGTT 
GGGGTTGGAT CTATGGCTGC TGATTCCATT GAAGTAGCTA GAAAACAGAG TGACTTAAAA 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF27 ( XM_011372048.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011370350.1 Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPt55372
Clone ID Related Accession (Same CDS sequence)	XM_011372048.2 , XM_011372048.1
Accession Version	XM_011372048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	kinesin-like protein KIF27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888980.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011372048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA TACCAGTAAA AGTTGCTGTA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
CTTCATAATC ATCAAGTTTG TGTGAGAGTT ATTCCAAACA CCCAGCAAAT TATTATTGGG 
AGAGATAGAA TCTTTACTTT CGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT 
TATAATATGT GCATAAAGCC ACTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT 
TTTGCCTACG GTCAAACTGG ATCTGGGAAG ACATATACCA TTGGAGGAGG CCATGTTGCC 
TCAGTTGCGG AAGGTCAAAA GGGTATTATT CCTAGAGCTA TCCAAGAAAT ATTTCAAAAC 
ATCTCTGAAA ACCTTAGCAT TGACTTTACA ATAAAAGTGT CTTATATAGA AGTATATAAA 
GAAGACTTAA GAGATCTTTT AGAATTGGAG ACATCTATGA AGGACCTTCA CATACGAGAA 
GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAGT GCCAGGTGGA GAGTGCAGAT 
GAAGTGATGA GCTTCTTGGA GATGGGGAAT GCAGCCAGGC ATACAGGTAC CACCCAAATG 
AACGAACACT CCAGTAGGTC ACATGCAATT TTTACAATCA GCATTTGTCA AGTTGAGAAA 
AATACAGAGA CAGTTGAAGA TGGATCATGG TGTTCCCATC GACGTATTGT CTCAAAGTTC 
CACTTTGTAG ATTTGGCTGG ATCAGAAAGA GTAACCAAAA CGGGTAACAC TGGTGAACGG 
TTCAAAGAAT CCATTCAAAT CAATAGTGGG TTGCTGGCTT TAGGAAACGT AATAAGTGCT 
CTTGGGGACC CACGCAGGAA GAGTTCACAT ATTCCGTATA GGGATGCTAA AATTACCCGA 
CTACTTAAAG ATTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA 
TCCTCCTCGG ATTTTGACGA ATCTTTAAAT TCTCTCAAGT ACGCCAACAG AGCACGCAAC 
ATCAGAAACA AACCCACCTT AAACTTCAGC CCGGAGTCAG ATCGGATGGA CGAAATGGAA 
TTTGAGATTA AGTTGCTTCG AGAGGCTTTG CAAAGCCAGC AGGCTAGTAG TATCAGCCAA 
AGCAGCCAGA TCCATCGAGA GGGGACTCCT GATAAAAATA AGATCCATTG TCTTGAGGAG 
CAAGTAGCTC AGCTCCAAGG AGAATGTCTG GGTTACCAAA ACTGTATCGA GGAAGCCTTC 
ACCTTCCTAG TGGAGTTAAA AGATGCTGTT AGACTAAATC AGAAGCAGCA ACACAAACTG 
CAAGAGTGGC TTAATATGAC CCAGGAGGTC AGGAAGGCCA TTCTCACTTC GTTCAGAGGA 
AGCCGGGGCA TGGGAAATTT GGAAGAAGGA CCACAGCGTA GCACAGTTCT CCAGCTGAAA 
CGAGAGCTGA AGAAATGCCA GTGTGCTCTC GCTGCTGATG AAGTAGTATT TAATCAGAAG 
GAACTGGAGG TGAAGGAACT AAAGAATCAA GTGCAAATGA TGGTACAGGA AAACAAAGGA 
CATGTTATAT CTTTGAAAGA AGCGCAAAAA GTTAATAGAT TACAGAATGA AAAAATAATA 
GAACAACAAC TTCTTATGGA TCAGCTGAAT GAAGAACTAA CAAAACTTAA CCTGTCAATG 
ACTTCTTCAG TTAAGGAAAC TTGTGGAGAT GGGCCAGATG CCAGGATACC TGAAAAGAGA 
CCATATACTG TACCATTTGA TACTCACCTG GGGCATTATA TTTATGTCCC AGCAAGATCA 
GATGCCAGGA AGGTCTACAC AAGTCCTGCT GCGTACTCTC TGGATCGCAT AGTTGCTGGA 
TTCCGAACAC GAAGTCAGAT GCTGCTGGGT CATATAGAAG ACCAAGATGA AGTCATCCAC 
TGCCAGTTTT CTGATAACAG TGATGAGGAA GAATCAGAGG GCCAAGAGAA ATCTGATATT 
AGAACTAGAA GTCACTCATG GATTCAAAAG TCTGGCTCCC TTTGTTCTCT TGTTGAACTG 
AATGATATTC ATGATCAAAA ACAAAAGTCA AGTTTGGGGA ATGAAGATTT AAAGATTGAA 
TGTCTCCAGG AGAGTCAAGA GTTGAATTTG CAAAAATTAA GGAATTCAGA ACTCATACTT 
AATGAAGCAA AACAAAAAAT GAGAGAACTT ACAATTAACA TCAAGATGAA AGAAGATCTA 
ATTAAAGAAT TAATGAAAAC AGGTAATGAT GCCAAGTCTG TAAGCAAACA GTATTCTCTG 
AAAGTAACAA AACTTGCACA TGAAGCAGAA CAGGCAAAAG TGGAATTAAT TGAAACAGAA 
AAACAACTAC AGGAACTGGA AAATAAAGAT CTTTCTGATG TTGCTTTGAA AGTAAAATTA 
CAGAGAGAGT TCCGTAAAAA GATGGATGCC GCAAAGCTAA AAGTCCAGGT CTTACAGAAG 
AAGCAACAAA ATAGTAAGAA ATTGGCATCG CTTTCGATCC AAAATGAGAA ACGTGCTAAT 
GAACTAGAGC AGAGTGTAGA TCACATGAAA TATCAAAAGG TACAGCTGCA AAAAAGACTT 
CGAGAAGAAA ATGAAAAAAG GAAGCAACTG GATACAGAAA TTAAGCGGGA TCAACAAAAA 
ATCAAAGAAC TAAAGTTAAA AACAGAACCA GAAGAAGGTT TAAAACTGAA AGCTGAGGAC 
CTTGATGCAT TGAAGATGAA AAGGAGAAAA GGTTCGTTTG GAAGTATAGA CCAACTCCAG 
AAATTGGATG AGCAAAAGAA ATGGTTAGAT GAAGAAGTAG AAAAAGTTCT GAACCAACGC 
CAAGAATTAG AGGAGCTGGA AGAAGACTTA AAGAAACGGG AGGCTATAGT TTCTAAAAAG 
GAGGCCCTGT TACAGGAGAA GAGCCACCTG GAGAATAAGA AGTTGAGATC TAGTCAGGCC 
TTAAACACAG ATAGTTTGAA AATATCAACT CGCCTGAACT TGCTGGACCA AGAGTTGTCT 
GAAAAGAATG TACAGCTCCA GAGCAGCACA GCTGAGGAGA AAATAAAGAT TTCAGAACAA 
GTCCAAGCCC TCCAGAAAGA AAAAGATCAG CTACAGAGAA GAAGAAACAG TGTGGATGAG 
AAACTTAAAA ATGGTAGCGT GTTATCACCT GAAGAAGAAC ATATTCTTTT CCAACTTGAA 
GAAGGGATTG AAGCTTTGGA AGCTGCAATT GAATACAAGA ATGAAAGTAT CCAGAGTCGC 
CAGAACTCGC TCAGAGCTTC ATTCCAAAAC CTCTCTCATA GTGAAGCAAA TGTCTTGGAA 
AAACTCATTG GCCTGAATCC TATTGAGATT AGAGCTATTC TTTTCAGATA CTTCAATAAG 
GTGGTTAATT TGCGAGAAGC TGAACGGAAA CTACAGTTAC AGAATAAAGA AATTAAAATG 
AAGATTCTGG AACGGGATAA TGTGGTTCGT GAGTTAGAAT CTGCACTGGA GCATCTGAAA 
TTGCAATGTG ACCGAAGACT GACCCTCCAG CAAAAGGAAC ATGAGCAAAA AATGCAGTTA 
CTATTACATC ATTTCCAAGA GCAAGATGGG GAAGGCATCG TGGAAACTTT AAAGACATAT 
GAAGATAAAA TCCAACAGCT AGAAAAAGAT CTTTATTTCT ATAAGAAAAC TAGCAGAGAT 
CTTAAGAAGA AACTTAAGGA ACTGTTAGGG GAAGCAATTC GAAGGCAACT AGTACCATCT 
GAACATCATT ATGTTGGAGA TGGAGTCCTG AACCCAGAAG AAGCAGGCAC GGTTTCAGAA 
GAATTAAAAT GGGCATCTAA AACTGAAAGT ATGAAGTTAA GTGGAAAAGA AAGAGAATTA 
AACAGTTCAG CAAGTAGCTT AAGAACACAA CGAAATCCTC AGAAGCTCTG GGAAGATGGC 
CCAGAATTAC CTCCAATTTA TAGCTCTTTA GCACCCACTA GTGGGCATTT GTTAAGCAAT 
GAGGATAAAA CAGAAGCAGA TGAAAATCAG ATTACAAAAA CTCACAGTTG CACATCACCC 
CAAATTCAGC CAGTGGGAAA TGTGGGACAG ATTCATGGTG TCACACCTGT AAAGTTGTGT 
CGCAAAGAGT TACGTCAGAT TTCTGCCGTG GAACTCTCAT TACGACGTTC CAGTCTTGGA 
GTTGGGGTTG GATCTATGGC TGCTGATTCC ATTGAAGTAG CTAGAAAACA GAGTGACTTA 
AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011370350.1
CDS	226..4434
Translation	MEEIPVKVAVRIRPLLCKEVLHNHQVCVRVIPNTQQIIIGRDRIFTFDFVFGKNSTQDEVYNMCIKPLVLSLIEGYNATVFAYGQTGSGKTYTIGGGHVASVAEGQKGIIPRAIQEIFQNISENLSIDFTIKVSYIEVYKEDLRDLLELETSMKDLHIREDEKGNTVIVGAKECQVESADEVMSFLEMGNAARHTGTTQMNEHSSRSHAIFTISICQVEKNTETVEDGSWCSHRRIVSKFHFVDLAGSERVTKTGNTGERFKESIQINSGLLALGNVISALGDPRRKSSHIPYRDAKITRLLKDSLGGSAKTVMITCVSPSSSDFDESLNSLKYANRARNIRNKPTLNFSPESDRMDEMEFEIKLLREALQSQQASSISQSSQIHREGTPDKNKIHCLEEQVAQLQGECLGYQNCIEEAFTFLVELKDAVRLNQKQQHKLQEWLNMTQEVRKAILTSFRGSRGMGNLEEGPQRSTVLQLKRELKKCQCALAADEVVFNQKELEVKELKNQVQMMVQENKGHVISLKEAQKVNRLQNEKIIEQQLLMDQLNEELTKLNLSMTSSVKETCGDGPDARIPEKRPYTVPFDTHLGHYIYVPARSDARKVYTSPAAYSLDRIVAGFRTRSQMLLGHIEDQDEVIHCQFSDNSDEEESEGQEKSDIRTRSHSWIQKSGSLCSLVELNDIHDQKQKSSLGNEDLKIECLQESQELNLQKLRNSELILNEAKQKMRELTINIKMKEDLIKELMKTGNDAKSVSKQYSLKVTKLAHEAEQAKVELIETEKQLQELENKDLSDVALKVKLQREFRKKMDAAKLKVQVLQKKQQNSKKLASLSIQNEKRANELEQSVDHMKYQKVQLQKRLREENEKRKQLDTEIKRDQQKIKELKLKTEPEEGLKLKAEDLDALKMKRRKGSFGSIDQLQKLDEQKKWLDEEVEKVLNQRQELEELEEDLKKREAIVSKKEALLQEKSHLENKKLRSSQALNTDSLKISTRLNLLDQELSEKNVQLQSSTAEEKIKISEQVQALQKEKDQLQRRRNSVDEKLKNGSVLSPEEEHILFQLEEGIEALEAAIEYKNESIQSRQNSLRASFQNLSHSEANVLEKLIGLNPIEIRAILFRYFNKVVNLREAERKLQLQNKEIKMKILERDNVVRELESALEHLKLQCDRRLTLQQKEHEQKMQLLLHHFQEQDGEGIVETLKTYEDKIQQLEKDLYFYKKTSRDLKKKLKELLGEAIRRQLVPSEHHYVGDGVLNPEEAGTVSEELKWASKTESMKLSGKERELNSSASSLRTQRNPQKLWEDGPELPPIYSSLAPTSGHLLSNEDKTEADENQITKTHSCTSPQIQPVGNVGQIHGVTPVKLCRKELRQISAVELSLRRSSLGVGVGSMAADSIEVARKQSDLKT

Target ORF information:

RefSeq Version	XM_011372048.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011372048.2

ORF Insert Sequence:

ATGGAAGAAA TACCAGTAAA AGTTGCTGTA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
CTTCATAATC ATCAAGTTTG TGTGAGAGTT ATTCCAAACA CCCAGCAAAT TATTATTGGG 
AGAGATAGAA TCTTTACTTT CGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT 
TATAATATGT GCATAAAGCC ACTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT 
TTTGCCTACG GTCAAACTGG ATCTGGGAAG ACATATACCA TTGGAGGAGG CCATGTTGCC 
TCAGTTGCGG AAGGTCAAAA GGGTATTATT CCTAGAGCTA TCCAAGAAAT ATTTCAAAAC 
ATCTCTGAAA ACCTTAGCAT TGACTTTACA ATAAAAGTGT CTTATATAGA AGTATATAAA 
GAAGACTTAA GAGATCTTTT AGAATTGGAG ACATCTATGA AGGACCTTCA CATACGAGAA 
GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAGT GCCAGGTGGA GAGTGCAGAT 
GAAGTGATGA GCTTCTTGGA GATGGGGAAT GCAGCCAGGC ATACAGGTAC CACCCAAATG 
AACGAACACT CCAGTAGGTC ACATGCAATT TTTACAATCA GCATTTGTCA AGTTGAGAAA 
AATACAGAGA CAGTTGAAGA TGGATCATGG TGTTCCCATC GACGTATTGT CTCAAAGTTC 
CACTTTGTAG ATTTGGCTGG ATCAGAAAGA GTAACCAAAA CGGGTAACAC TGGTGAACGG 
TTCAAAGAAT CCATTCAAAT CAATAGTGGG TTGCTGGCTT TAGGAAACGT AATAAGTGCT 
CTTGGGGACC CACGCAGGAA GAGTTCACAT ATTCCGTATA GGGATGCTAA AATTACCCGA 
CTACTTAAAG ATTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA 
TCCTCCTCGG ATTTTGACGA ATCTTTAAAT TCTCTCAAGT ACGCCAACAG AGCACGCAAC 
ATCAGAAACA AACCCACCTT AAACTTCAGC CCGGAGTCAG ATCGGATGGA CGAAATGGAA 
TTTGAGATTA AGTTGCTTCG AGAGGCTTTG CAAAGCCAGC AGGCTAGTAG TATCAGCCAA 
AGCAGCCAGA TCCATCGAGA GGGGACTCCT GATAAAAATA AGATCCATTG TCTTGAGGAG 
CAAGTAGCTC AGCTCCAAGG AGAATGTCTG GGTTACCAAA ACTGTATCGA GGAAGCCTTC 
ACCTTCCTAG TGGAGTTAAA AGATGCTGTT AGACTAAATC AGAAGCAGCA ACACAAACTG 
CAAGAGTGGC TTAATATGAC CCAGGAGGTC AGGAAGGCCA TTCTCACTTC GTTCAGAGGA 
AGCCGGGGCA TGGGAAATTT GGAAGAAGGA CCACAGCGTA GCACAGTTCT CCAGCTGAAA 
CGAGAGCTGA AGAAATGCCA GTGTGCTCTC GCTGCTGATG AAGTAGTATT TAATCAGAAG 
GAACTGGAGG TGAAGGAACT AAAGAATCAA GTGCAAATGA TGGTACAGGA AAACAAAGGA 
CATGTTATAT CTTTGAAAGA AGCGCAAAAA GTTAATAGAT TACAGAATGA AAAAATAATA 
GAACAACAAC TTCTTATGGA TCAGCTGAAT GAAGAACTAA CAAAACTTAA CCTGTCAATG 
ACTTCTTCAG TTAAGGAAAC TTGTGGAGAT GGGCCAGATG CCAGGATACC TGAAAAGAGA 
CCATATACTG TACCATTTGA TACTCACCTG GGGCATTATA TTTATGTCCC AGCAAGATCA 
GATGCCAGGA AGGTCTACAC AAGTCCTGCT GCGTACTCTC TGGATCGCAT AGTTGCTGGA 
TTCCGAACAC GAAGTCAGAT GCTGCTGGGT CATATAGAAG ACCAAGATGA AGTCATCCAC 
TGCCAGTTTT CTGATAACAG TGATGAGGAA GAATCAGAGG GCCAAGAGAA ATCTGATATT 
AGAACTAGAA GTCACTCATG GATTCAAAAG TCTGGCTCCC TTTGTTCTCT TGTTGAACTG 
AATGATATTC ATGATCAAAA ACAAAAGTCA AGTTTGGGGA ATGAAGATTT AAAGATTGAA 
TGTCTCCAGG AGAGTCAAGA GTTGAATTTG CAAAAATTAA GGAATTCAGA ACTCATACTT 
AATGAAGCAA AACAAAAAAT GAGAGAACTT ACAATTAACA TCAAGATGAA AGAAGATCTA 
ATTAAAGAAT TAATGAAAAC AGGTAATGAT GCCAAGTCTG TAAGCAAACA GTATTCTCTG 
AAAGTAACAA AACTTGCACA TGAAGCAGAA CAGGCAAAAG TGGAATTAAT TGAAACAGAA 
AAACAACTAC AGGAACTGGA AAATAAAGAT CTTTCTGATG TTGCTTTGAA AGTAAAATTA 
CAGAGAGAGT TCCGTAAAAA GATGGATGCC GCAAAGCTAA AAGTCCAGGT CTTACAGAAG 
AAGCAACAAA ATAGTAAGAA ATTGGCATCG CTTTCGATCC AAAATGAGAA ACGTGCTAAT 
GAACTAGAGC AGAGTGTAGA TCACATGAAA TATCAAAAGG TACAGCTGCA AAAAAGACTT 
CGAGAAGAAA ATGAAAAAAG GAAGCAACTG GATACAGAAA TTAAGCGGGA TCAACAAAAA 
ATCAAAGAAC TAAAGTTAAA AACAGAACCA GAAGAAGGTT TAAAACTGAA AGCTGAGGAC 
CTTGATGCAT TGAAGATGAA AAGGAGAAAA GGTTCGTTTG GAAGTATAGA CCAACTCCAG 
AAATTGGATG AGCAAAAGAA ATGGTTAGAT GAAGAAGTAG AAAAAGTTCT GAACCAACGC 
CAAGAATTAG AGGAGCTGGA AGAAGACTTA AAGAAACGGG AGGCTATAGT TTCTAAAAAG 
GAGGCCCTGT TACAGGAGAA GAGCCACCTG GAGAATAAGA AGTTGAGATC TAGTCAGGCC 
TTAAACACAG ATAGTTTGAA AATATCAACT CGCCTGAACT TGCTGGACCA AGAGTTGTCT 
GAAAAGAATG TACAGCTCCA GAGCAGCACA GCTGAGGAGA AAATAAAGAT TTCAGAACAA 
GTCCAAGCCC TCCAGAAAGA AAAAGATCAG CTACAGAGAA GAAGAAACAG TGTGGATGAG 
AAACTTAAAA ATGGTAGCGT GTTATCACCT GAAGAAGAAC ATATTCTTTT CCAACTTGAA 
GAAGGGATTG AAGCTTTGGA AGCTGCAATT GAATACAAGA ATGAAAGTAT CCAGAGTCGC 
CAGAACTCGC TCAGAGCTTC ATTCCAAAAC CTCTCTCATA GTGAAGCAAA TGTCTTGGAA 
AAACTCATTG GCCTGAATCC TATTGAGATT AGAGCTATTC TTTTCAGATA CTTCAATAAG 
GTGGTTAATT TGCGAGAAGC TGAACGGAAA CTACAGTTAC AGAATAAAGA AATTAAAATG 
AAGATTCTGG AACGGGATAA TGTGGTTCGT GAGTTAGAAT CTGCACTGGA GCATCTGAAA 
TTGCAATGTG ACCGAAGACT GACCCTCCAG CAAAAGGAAC ATGAGCAAAA AATGCAGTTA 
CTATTACATC ATTTCCAAGA GCAAGATGGG GAAGGCATCG TGGAAACTTT AAAGACATAT 
GAAGATAAAA TCCAACAGCT AGAAAAAGAT CTTTATTTCT ATAAGAAAAC TAGCAGAGAT 
CTTAAGAAGA AACTTAAGGA ACTGTTAGGG GAAGCAATTC GAAGGCAACT AGTACCATCT 
GAACATCATT ATGTTGGAGA TGGAGTCCTG AACCCAGAAG AAGCAGGCAC GGTTTCAGAA 
GAATTAAAAT GGGCATCTAA AACTGAAAGT ATGAAGTTAA GTGGAAAAGA AAGAGAATTA 
AACAGTTCAG CAAGTAGCTT AAGAACACAA CGAAATCCTC AGAAGCTCTG GGAAGATGGC 
CCAGAATTAC CTCCAATTTA TAGCTCTTTA GCACCCACTA GTGGGCATTT GTTAAGCAAT 
GAGGATAAAA CAGAAGCAGA TGAAAATCAG ATTACAAAAA CTCACAGTTG CACATCACCC 
CAAATTCAGC CAGTGGGAAA TGTGGGACAG ATTCATGGTG TCACACCTGT AAAGTTGTGT 
CGCAAAGAGT TACGTCAGAT TTCTGCCGTG GAACTCTCAT TACGACGTTC CAGTCTTGGA 
GTTGGGGTTG GATCTATGGC TGCTGATTCC ATTGAAGTAG CTAGAAAACA GAGTGACTTA 
AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF27 ( XM_011372048.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011370350.1 Pteropus vampyrus kinesin family member 27 (KIF27), mRNA.

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CloneID	OPt55372
Clone ID Related Accession (Same CDS sequence)	XM_011372048.2 , XM_011372048.1
Accession Version	XM_011372048.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	kinesin-like protein KIF27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888980.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA TACCAGTAAA AGTTGCTGTA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
CTTCATAATC ATCAAGTTTG TGTGAGAGTT ATTCCAAACA CCCAGCAAAT TATTATTGGG 
AGAGATAGAA TCTTTACTTT CGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT 
TATAATATGT GCATAAAGCC ACTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT 
TTTGCCTACG GTCAAACTGG ATCTGGGAAG ACATATACCA TTGGAGGAGG CCATGTTGCC 
TCAGTTGCGG AAGGTCAAAA GGGTATTATT CCTAGAGCTA TCCAAGAAAT ATTTCAAAAC 
ATCTCTGAAA ACCTTAGCAT TGACTTTACA ATAAAAGTGT CTTATATAGA AGTATATAAA 
GAAGACTTAA GAGATCTTTT AGAATTGGAG ACATCTATGA AGGACCTTCA CATACGAGAA 
GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAGT GCCAGGTGGA GAGTGCAGAT 
GAAGTGATGA GCTTCTTGGA GATGGGGAAT GCAGCCAGGC ATACAGGTAC CACCCAAATG 
AACGAACACT CCAGTAGGTC ACATGCAATT TTTACAATCA GCATTTGTCA AGTTGAGAAA 
AATACAGAGA CAGTTGAAGA TGGATCATGG TGTTCCCATC GACGTATTGT CTCAAAGTTC 
CACTTTGTAG ATTTGGCTGG ATCAGAAAGA GTAACCAAAA CGGGTAACAC TGGTGAACGG 
TTCAAAGAAT CCATTCAAAT CAATAGTGGG TTGCTGGCTT TAGGAAACGT AATAAGTGCT 
CTTGGGGACC CACGCAGGAA GAGTTCACAT ATTCCGTATA GGGATGCTAA AATTACCCGA 
CTACTTAAAG ATTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA 
TCCTCCTCGG ATTTTGACGA ATCTTTAAAT TCTCTCAAGT ACGCCAACAG AGCACGCAAC 
ATCAGAAACA AACCCACCTT AAACTTCAGC CCGGAGTCAG ATCGGATGGA CGAAATGGAA 
TTTGAGATTA AGTTGCTTCG AGAGGCTTTG CAAAGCCAGC AGGCTAGTAG TATCAGCCAA 
AGCAGCCAGA TCCATCGAGA GGGGACTCCT GATAAAAATA AGATCCATTG TCTTGAGGAG 
CAAGTAGCTC AGCTCCAAGG AGAATGTCTG GGTTACCAAA ACTGTATCGA GGAAGCCTTC 
ACCTTCCTAG TGGAGTTAAA AGATGCTGTT AGACTAAATC AGAAGCAGCA ACACAAACTG 
CAAGAGTGGC TTAATATGAC CCAGGAGGTC AGGAAGGCCA TTCTCACTTC GTTCAGAGGA 
AGCCGGGGCA TGGGAAATTT GGAAGAAGGA CCACAGCGTA GCACAGTTCT CCAGCTGAAA 
CGAGAGCTGA AGAAATGCCA GTGTGCTCTC GCTGCTGATG AAGTAGTATT TAATCAGAAG 
GAACTGGAGG TGAAGGAACT AAAGAATCAA GTGCAAATGA TGGTACAGGA AAACAAAGGA 
CATGTTATAT CTTTGAAAGA AGCGCAAAAA GTTAATAGAT TACAGAATGA AAAAATAATA 
GAACAACAAC TTCTTATGGA TCAGCTGAAT GAAGAACTAA CAAAACTTAA CCTGTCAATG 
ACTTCTTCAG TTAAGGAAAC TTGTGGAGAT GGGCCAGATG CCAGGATACC TGAAAAGAGA 
CCATATACTG TACCATTTGA TACTCACCTG GGGCATTATA TTTATGTCCC AGCAAGATCA 
GATGCCAGGA AGGTCTACAC AAGTCCTGCT GCGTACTCTC TGGATCGCAT AGTTGCTGGA 
TTCCGAACAC GAAGTCAGAT GCTGCTGGGT CATATAGAAG ACCAAGATGA AGTCATCCAC 
TGCCAGTTTT CTGATAACAG TGATGAGGAA GAATCAGAGG GCCAAGAGAA ATCTGATATT 
AGAACTAGAA GTCACTCATG GATTCAAAAG TCTGGCTCCC TTTGTTCTCT TGTTGAACTG 
AATGATATTC ATGATCAAAA ACAAAAGTCA AGTTTGGGGA ATGAAGATTT AAAGATTGAA 
TGTCTCCAGG AGAGTCAAGA GTTGAATTTG CAAAAATTAA GGAATTCAGA ACTCATACTT 
AATGAAGCAA AACAAAAAAT GAGAGAACTT ACAATTAACA TCAAGATGAA AGAAGATCTA 
ATTAAAGAAT TAATGAAAAC AGGTAATGAT GCCAAGTCTG TAAGCAAACA GTATTCTCTG 
AAAGTAACAA AACTTGCACA TGAAGCAGAA CAGGCAAAAG TGGAATTAAT TGAAACAGAA 
AAACAACTAC AGGAACTGGA AAATAAAGAT CTTTCTGATG TTGCTTTGAA AGTAAAATTA 
CAGAGAGAGT TCCGTAAAAA GATGGATGCC GCAAAGCTAA AAGTCCAGGT CTTACAGAAG 
AAGCAACAAA ATAGTAAGAA ATTGGCATCG CTTTCGATCC AAAATGAGAA ACGTGCTAAT 
GAACTAGAGC AGAGTGTAGA TCACATGAAA TATCAAAAGG TACAGCTGCA AAAAAGACTT 
CGAGAAGAAA ATGAAAAAAG GAAGCAACTG GATACAGAAA TTAAGCGGGA TCAACAAAAA 
ATCAAAGAAC TAAAGTTAAA AACAGAACCA GAAGAAGGTT TAAAACTGAA AGCTGAGGAC 
CTTGATGCAT TGAAGATGAA AAGGAGAAAA GGTTCGTTTG GAAGTATAGA CCAACTCCAG 
AAATTGGATG AGCAAAAGAA ATGGTTAGAT GAAGAAGTAG AAAAAGTTCT GAACCAACGC 
CAAGAATTAG AGGAGCTGGA AGAAGACTTA AAGAAACGGG AGGCTATAGT TTCTAAAAAG 
GAGGCCCTGT TACAGGAGAA GAGCCACCTG GAGAATAAGA AGTTGAGATC TAGTCAGGCC 
TTAAACACAG ATAGTTTGAA AATATCAACT CGCCTGAACT TGCTGGACCA AGAGTTGTCT 
GAAAAGAATG TACAGCTCCA GAGCAGCACA GCTGAGGAGA AAATAAAGAT TTCAGAACAA 
GTCCAAGCCC TCCAGAAAGA AAAAGATCAG CTACAGAGAA GAAGAAACAG TGTGGATGAG 
AAACTTAAAA ATGGTAGCGT GTTATCACCT GAAGAAGAAC ATATTCTTTT CCAACTTGAA 
GAAGGGATTG AAGCTTTGGA AGCTGCAATT GAATACAAGA ATGAAAGTAT CCAGAGTCGC 
CAGAACTCGC TCAGAGCTTC ATTCCAAAAC CTCTCTCATA GTGAAGCAAA TGTCTTGGAA 
AAACTCATTG GCCTGAATCC TATTGAGATT AGAGCTATTC TTTTCAGATA CTTCAATAAG 
GTGGTTAATT TGCGAGAAGC TGAACGGAAA CTACAGTTAC AGAATAAAGA AATTAAAATG 
AAGATTCTGG AACGGGATAA TGTGGTTCGT GAGTTAGAAT CTGCACTGGA GCATCTGAAA 
TTGCAATGTG ACCGAAGACT GACCCTCCAG CAAAAGGAAC ATGAGCAAAA AATGCAGTTA 
CTATTACATC ATTTCCAAGA GCAAGATGGG GAAGGCATCG TGGAAACTTT AAAGACATAT 
GAAGATAAAA TCCAACAGCT AGAAAAAGAT CTTTATTTCT ATAAGAAAAC TAGCAGAGAT 
CTTAAGAAGA AACTTAAGGA ACTGTTAGGG GAAGCAATTC GAAGGCAACT AGTACCATCT 
GAACATCATT ATGTTGGAGA TGGAGTCCTG AACCCAGAAG AAGCAGGCAC GGTTTCAGAA 
GAATTAAAAT GGGCATCTAA AACTGAAAGT ATGAAGTTAA GTGGAAAAGA AAGAGAATTA 
AACAGTTCAG CAAGTAGCTT AAGAACACAA CGAAATCCTC AGAAGCTCTG GGAAGATGGC 
CCAGAATTAC CTCCAATTTA TAGCTCTTTA GCACCCACTA GTGGGCATTT GTTAAGCAAT 
GAGGATAAAA CAGAAGCAGA TGAAAATCAG ATTACAAAAA CTCACAGTTG CACATCACCC 
CAAATTCAGC CAGTGGGAAA TGTGGGACAG ATTCATGGTG TCACACCTGT AAAGTTGTGT 
CGCAAAGAGT TACGTCAGAT TTCTGCCGTG GAACTCTCAT TACGACGTTC CAGTCTTGGA 
GTTGGGGTTG GATCTATGGC TGCTGATTCC ATTGAAGTAG CTAGAAAACA GAGTGACTTA 
AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011370350.1
CDS	231..4439
Translation	MEEIPVKVAVRIRPLLCKEVLHNHQVCVRVIPNTQQIIIGRDRIFTFDFVFGKNSTQDEVYNMCIKPLVLSLIEGYNATVFAYGQTGSGKTYTIGGGHVASVAEGQKGIIPRAIQEIFQNISENLSIDFTIKVSYIEVYKEDLRDLLELETSMKDLHIREDEKGNTVIVGAKECQVESADEVMSFLEMGNAARHTGTTQMNEHSSRSHAIFTISICQVEKNTETVEDGSWCSHRRIVSKFHFVDLAGSERVTKTGNTGERFKESIQINSGLLALGNVISALGDPRRKSSHIPYRDAKITRLLKDSLGGSAKTVMITCVSPSSSDFDESLNSLKYANRARNIRNKPTLNFSPESDRMDEMEFEIKLLREALQSQQASSISQSSQIHREGTPDKNKIHCLEEQVAQLQGECLGYQNCIEEAFTFLVELKDAVRLNQKQQHKLQEWLNMTQEVRKAILTSFRGSRGMGNLEEGPQRSTVLQLKRELKKCQCALAADEVVFNQKELEVKELKNQVQMMVQENKGHVISLKEAQKVNRLQNEKIIEQQLLMDQLNEELTKLNLSMTSSVKETCGDGPDARIPEKRPYTVPFDTHLGHYIYVPARSDARKVYTSPAAYSLDRIVAGFRTRSQMLLGHIEDQDEVIHCQFSDNSDEEESEGQEKSDIRTRSHSWIQKSGSLCSLVELNDIHDQKQKSSLGNEDLKIECLQESQELNLQKLRNSELILNEAKQKMRELTINIKMKEDLIKELMKTGNDAKSVSKQYSLKVTKLAHEAEQAKVELIETEKQLQELENKDLSDVALKVKLQREFRKKMDAAKLKVQVLQKKQQNSKKLASLSIQNEKRANELEQSVDHMKYQKVQLQKRLREENEKRKQLDTEIKRDQQKIKELKLKTEPEEGLKLKAEDLDALKMKRRKGSFGSIDQLQKLDEQKKWLDEEVEKVLNQRQELEELEEDLKKREAIVSKKEALLQEKSHLENKKLRSSQALNTDSLKISTRLNLLDQELSEKNVQLQSSTAEEKIKISEQVQALQKEKDQLQRRRNSVDEKLKNGSVLSPEEEHILFQLEEGIEALEAAIEYKNESIQSRQNSLRASFQNLSHSEANVLEKLIGLNPIEIRAILFRYFNKVVNLREAERKLQLQNKEIKMKILERDNVVRELESALEHLKLQCDRRLTLQQKEHEQKMQLLLHHFQEQDGEGIVETLKTYEDKIQQLEKDLYFYKKTSRDLKKKLKELLGEAIRRQLVPSEHHYVGDGVLNPEEAGTVSEELKWASKTESMKLSGKERELNSSASSLRTQRNPQKLWEDGPELPPIYSSLAPTSGHLLSNEDKTEADENQITKTHSCTSPQIQPVGNVGQIHGVTPVKLCRKELRQISAVELSLRRSSLGVGVGSMAADSIEVARKQSDLKT

Target ORF information:

RefSeq Version	XM_011372048.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus kinesin family member 27 (KIF27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011372048.1

ORF Insert Sequence:

ATGGAAGAAA TACCAGTAAA AGTTGCTGTA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
CTTCATAATC ATCAAGTTTG TGTGAGAGTT ATTCCAAACA CCCAGCAAAT TATTATTGGG 
AGAGATAGAA TCTTTACTTT CGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT 
TATAATATGT GCATAAAGCC ACTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT 
TTTGCCTACG GTCAAACTGG ATCTGGGAAG ACATATACCA TTGGAGGAGG CCATGTTGCC 
TCAGTTGCGG AAGGTCAAAA GGGTATTATT CCTAGAGCTA TCCAAGAAAT ATTTCAAAAC 
ATCTCTGAAA ACCTTAGCAT TGACTTTACA ATAAAAGTGT CTTATATAGA AGTATATAAA 
GAAGACTTAA GAGATCTTTT AGAATTGGAG ACATCTATGA AGGACCTTCA CATACGAGAA 
GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAGT GCCAGGTGGA GAGTGCAGAT 
GAAGTGATGA GCTTCTTGGA GATGGGGAAT GCAGCCAGGC ATACAGGTAC CACCCAAATG 
AACGAACACT CCAGTAGGTC ACATGCAATT TTTACAATCA GCATTTGTCA AGTTGAGAAA 
AATACAGAGA CAGTTGAAGA TGGATCATGG TGTTCCCATC GACGTATTGT CTCAAAGTTC 
CACTTTGTAG ATTTGGCTGG ATCAGAAAGA GTAACCAAAA CGGGTAACAC TGGTGAACGG 
TTCAAAGAAT CCATTCAAAT CAATAGTGGG TTGCTGGCTT TAGGAAACGT AATAAGTGCT 
CTTGGGGACC CACGCAGGAA GAGTTCACAT ATTCCGTATA GGGATGCTAA AATTACCCGA 
CTACTTAAAG ATTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA 
TCCTCCTCGG ATTTTGACGA ATCTTTAAAT TCTCTCAAGT ACGCCAACAG AGCACGCAAC 
ATCAGAAACA AACCCACCTT AAACTTCAGC CCGGAGTCAG ATCGGATGGA CGAAATGGAA 
TTTGAGATTA AGTTGCTTCG AGAGGCTTTG CAAAGCCAGC AGGCTAGTAG TATCAGCCAA 
AGCAGCCAGA TCCATCGAGA GGGGACTCCT GATAAAAATA AGATCCATTG TCTTGAGGAG 
CAAGTAGCTC AGCTCCAAGG AGAATGTCTG GGTTACCAAA ACTGTATCGA GGAAGCCTTC 
ACCTTCCTAG TGGAGTTAAA AGATGCTGTT AGACTAAATC AGAAGCAGCA ACACAAACTG 
CAAGAGTGGC TTAATATGAC CCAGGAGGTC AGGAAGGCCA TTCTCACTTC GTTCAGAGGA 
AGCCGGGGCA TGGGAAATTT GGAAGAAGGA CCACAGCGTA GCACAGTTCT CCAGCTGAAA 
CGAGAGCTGA AGAAATGCCA GTGTGCTCTC GCTGCTGATG AAGTAGTATT TAATCAGAAG 
GAACTGGAGG TGAAGGAACT AAAGAATCAA GTGCAAATGA TGGTACAGGA AAACAAAGGA 
CATGTTATAT CTTTGAAAGA AGCGCAAAAA GTTAATAGAT TACAGAATGA AAAAATAATA 
GAACAACAAC TTCTTATGGA TCAGCTGAAT GAAGAACTAA CAAAACTTAA CCTGTCAATG 
ACTTCTTCAG TTAAGGAAAC TTGTGGAGAT GGGCCAGATG CCAGGATACC TGAAAAGAGA 
CCATATACTG TACCATTTGA TACTCACCTG GGGCATTATA TTTATGTCCC AGCAAGATCA 
GATGCCAGGA AGGTCTACAC AAGTCCTGCT GCGTACTCTC TGGATCGCAT AGTTGCTGGA 
TTCCGAACAC GAAGTCAGAT GCTGCTGGGT CATATAGAAG ACCAAGATGA AGTCATCCAC 
TGCCAGTTTT CTGATAACAG TGATGAGGAA GAATCAGAGG GCCAAGAGAA ATCTGATATT 
AGAACTAGAA GTCACTCATG GATTCAAAAG TCTGGCTCCC TTTGTTCTCT TGTTGAACTG 
AATGATATTC ATGATCAAAA ACAAAAGTCA AGTTTGGGGA ATGAAGATTT AAAGATTGAA 
TGTCTCCAGG AGAGTCAAGA GTTGAATTTG CAAAAATTAA GGAATTCAGA ACTCATACTT 
AATGAAGCAA AACAAAAAAT GAGAGAACTT ACAATTAACA TCAAGATGAA AGAAGATCTA 
ATTAAAGAAT TAATGAAAAC AGGTAATGAT GCCAAGTCTG TAAGCAAACA GTATTCTCTG 
AAAGTAACAA AACTTGCACA TGAAGCAGAA CAGGCAAAAG TGGAATTAAT TGAAACAGAA 
AAACAACTAC AGGAACTGGA AAATAAAGAT CTTTCTGATG TTGCTTTGAA AGTAAAATTA 
CAGAGAGAGT TCCGTAAAAA GATGGATGCC GCAAAGCTAA AAGTCCAGGT CTTACAGAAG 
AAGCAACAAA ATAGTAAGAA ATTGGCATCG CTTTCGATCC AAAATGAGAA ACGTGCTAAT 
GAACTAGAGC AGAGTGTAGA TCACATGAAA TATCAAAAGG TACAGCTGCA AAAAAGACTT 
CGAGAAGAAA ATGAAAAAAG GAAGCAACTG GATACAGAAA TTAAGCGGGA TCAACAAAAA 
ATCAAAGAAC TAAAGTTAAA AACAGAACCA GAAGAAGGTT TAAAACTGAA AGCTGAGGAC 
CTTGATGCAT TGAAGATGAA AAGGAGAAAA GGTTCGTTTG GAAGTATAGA CCAACTCCAG 
AAATTGGATG AGCAAAAGAA ATGGTTAGAT GAAGAAGTAG AAAAAGTTCT GAACCAACGC 
CAAGAATTAG AGGAGCTGGA AGAAGACTTA AAGAAACGGG AGGCTATAGT TTCTAAAAAG 
GAGGCCCTGT TACAGGAGAA GAGCCACCTG GAGAATAAGA AGTTGAGATC TAGTCAGGCC 
TTAAACACAG ATAGTTTGAA AATATCAACT CGCCTGAACT TGCTGGACCA AGAGTTGTCT 
GAAAAGAATG TACAGCTCCA GAGCAGCACA GCTGAGGAGA AAATAAAGAT TTCAGAACAA 
GTCCAAGCCC TCCAGAAAGA AAAAGATCAG CTACAGAGAA GAAGAAACAG TGTGGATGAG 
AAACTTAAAA ATGGTAGCGT GTTATCACCT GAAGAAGAAC ATATTCTTTT CCAACTTGAA 
GAAGGGATTG AAGCTTTGGA AGCTGCAATT GAATACAAGA ATGAAAGTAT CCAGAGTCGC 
CAGAACTCGC TCAGAGCTTC ATTCCAAAAC CTCTCTCATA GTGAAGCAAA TGTCTTGGAA 
AAACTCATTG GCCTGAATCC TATTGAGATT AGAGCTATTC TTTTCAGATA CTTCAATAAG 
GTGGTTAATT TGCGAGAAGC TGAACGGAAA CTACAGTTAC AGAATAAAGA AATTAAAATG 
AAGATTCTGG AACGGGATAA TGTGGTTCGT GAGTTAGAAT CTGCACTGGA GCATCTGAAA 
TTGCAATGTG ACCGAAGACT GACCCTCCAG CAAAAGGAAC ATGAGCAAAA AATGCAGTTA 
CTATTACATC ATTTCCAAGA GCAAGATGGG GAAGGCATCG TGGAAACTTT AAAGACATAT 
GAAGATAAAA TCCAACAGCT AGAAAAAGAT CTTTATTTCT ATAAGAAAAC TAGCAGAGAT 
CTTAAGAAGA AACTTAAGGA ACTGTTAGGG GAAGCAATTC GAAGGCAACT AGTACCATCT 
GAACATCATT ATGTTGGAGA TGGAGTCCTG AACCCAGAAG AAGCAGGCAC GGTTTCAGAA 
GAATTAAAAT GGGCATCTAA AACTGAAAGT ATGAAGTTAA GTGGAAAAGA AAGAGAATTA 
AACAGTTCAG CAAGTAGCTT AAGAACACAA CGAAATCCTC AGAAGCTCTG GGAAGATGGC 
CCAGAATTAC CTCCAATTTA TAGCTCTTTA GCACCCACTA GTGGGCATTT GTTAAGCAAT 
GAGGATAAAA CAGAAGCAGA TGAAAATCAG ATTACAAAAA CTCACAGTTG CACATCACCC 
CAAATTCAGC CAGTGGGAAA TGTGGGACAG ATTCATGGTG TCACACCTGT AAAGTTGTGT 
CGCAAAGAGT TACGTCAGAT TTCTGCCGTG GAACTCTCAT TACGACGTTC CAGTCTTGGA 
GTTGGGGTTG GATCTATGGC TGCTGATTCC ATTGAAGTAG CTAGAAAACA GAGTGACTTA 
AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF27 ( XM_023537756.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023393524.1 Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPt73949
Clone ID Related Accession (Same CDS sequence)	XM_023537756.1
Accession Version	XM_023537756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	kinesin-like protein KIF27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888980.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGGG GCTTAACGCC AAGGGGCGGG GCTTGGGCGG TCGGACTCCC AGGCGGGAGC 
CGGCGCGTTG GCGCAGGGCT TTGCCGGTTG CCGGGAGAAC CCGTGACAAC AAAAGGCGCG 
GACGGCAGCG GCGGCGGCGG CGTGGCGGAG TTTAGCGGAG GAGTCCTGCG CTGCGCCCGC 
CGCCGGAGAC TGGGTTTTTC TTTAGCGGCC TCAGTTGCGG AAGGTCAAAA GGGTATTATT 
CCTAGAGCTA TCCAAGAAAT ATTTCAAAAC ATCTCTGAAA ACCTTAGCAT TGACTTTACA 
ATAAAAGTGT CTTATATAGA AGTATATAAA GAAGACTTAA GAGATCTTTT AGAATTGGAG 
ACATCTATGA AGGACCTTCA CATACGAGAA GATGAAAAAG GAAACACAGT GATTGTTGGG 
GCCAAGGAGT GCCAGGTGGA GAGTGCAGAT GAAGTGATGA GCTTCTTGGA GATGGGGAAT 
GCAGCCAGGC ATACAGGTAC CACCCAAATG AACGAACACT CCAGTAGGTC ACATGCAATT 
TTTACAATCA GCATTTGTCA AGTTGAGAAA AATACAGAGA CAGTTGAAGA TGGATCATGG 
TGTTCCCATC GACGTATTGT CTCAAAGTTC CACTTTGTAG ATTTGGCTGG ATCAGAAAGA 
GTAACCAAAA CGGGTAACAC TGGTGAACGG TTCAAAGAAT CCATTCAAAT CAATAGTGGG 
TTGCTGGCTT TAGGAAACGT AATAAGTGCT CTTGGGGACC CACGCAGGAA GAGTTCACAT 
ATTCCGTATA GGGATGCTAA AATTACCCGA CTACTTAAAG ATTCCCTGGG AGGCAGTGCC 
AAGACTGTCA TGATCACTTG TGTCAGCCCA TCCTCCTCGG ATTTTGACGA ATCTTTAAAT 
TCTCTCAAGT ACGCCAACAG AGCACGCAAC ATCAGAAACA AACCCACCTT AAACTTCAGC 
CCGGAGTCAG ATCGGATGGA CGAAATGGAA TTTGAGATTA AGTTGCTTCG AGAGGCTTTG 
CAAAGCCAGC AGGCTAGTAG TATCAGCCAA AGCAGCCAGA TCCATCGAGA GGGGACTCCT 
GATAAAAATA AGATCCATTG TCTTGAGGAG CAAGTAGCTC AGCTCCAAGG AGAATGTCTG 
GGTTACCAAA ACTGTATCGA GGAAGCCTTC ACCTTCCTAG TGGAGTTAAA AGATGCTGTT 
AGACTAAATC AGAAGCAGCA ACACAAACTG CAAGAGTGGC TTAATATGAC CCAGGAGGTC 
AGGAAGGCCA TTCTCACTTC GTTCAGAGGA AGCCGGGGCA TGGGAAATTT GGAAGAAGGA 
CCACAGCGTA GCACAGTTCT CCAGCTGAAA CGAGAGCTGA AGAAATGCCA GTGTGCTCTC 
GCTGCTGATG AAGTAGTATT TAATCAGAAG GAACTGGAGG TGAAGGAACT AAAGAATCAA 
GTGCAAATGA TGGTACAGGA AAACAAAGGA CATGTTATAT CTTTGAAAGA AGCGCAAAAA 
GTTAATAGAT TACAGAATGA AAAAATAATA GAACAACAAC TTCTTATGGA TCAGCTGAAT 
GAAGAACTAA CAAAACTTAA CCTGTCAATG ACTTCTTCAG TTAAGGAAAC TTGTGGAGAT 
GGGCCAGATG CCAGGATACC TGAAAAGAGA CCATATACTG TACCATTTGA TACTCACCTG 
GGGCATTATA TTTATGTCCC AGCAAGATCA GATGCCAGGA AGGTCTACAC AAGTCCTGCT 
GCGTACTCTC TGGATCGCAT AGTTGCTGGA TTCCGAACAC GAAGTCAGAT GCTGCTGGGT 
CATATAGAAG ACCAAGATGA AGTCATCCAC TGCCAGTTTT CTGATAACAG TGATGAGGAA 
GAATCAGAGG GCCAAGAGAA ATCTGATATT AGAACTAGAA GTCACTCATG GATTCAAAAG 
TCTGGCTCCC TTTGTTCTCT TGTTGAACTG AATGATATTC ATGATCAAAA ACAAAAGTCA 
AGTTTGGGGA ATGAAGATTT AAAGATTGAA TGTCTCCAGG AGAGTCAAGA GTTGAATTTG 
CAAAAATTAA GGAATTCAGA ACTCATACTT AATGAAGCAA AACAAAAAAT GAGAGAACTT 
ACAATTAACA TCAAGATGAA AGAAGATCTA ATTAAAGAAT TAATGAAAAC AGGTAATGAT 
GCCAAGTCTG TAAGCAAACA GTATTCTCTG AAAGTAACAA AACTTGCACA TGAAGCAGAA 
CAGGCAAAAG TGGAATTAAT TGAAACAGAA AAACAACTAC AGGAACTGGA AAATAAAGAT 
CTTTCTGATG TTGCTTTGAA AGTAAAATTA CAGAGAGAGT TCCGTAAAAA GATGGATGCC 
GCAAAGCTAA AAGTCCAGGT CTTACAGAAG AAGCAACAAA ATAGTAAGAA ATTGGCATCG 
CTTTCGATCC AAAATGAGAA ACGTGCTAAT GAACTAGAGC AGAGTGTAGA TCACATGAAA 
TATCAAAAGG TACAGCTGCA AAAAAGACTT CGAGAAGAAA ATGAAAAAAG GAAGCAACTG 
GATACAGAAA TTAAGCGGGA TCAACAAAAA ATCAAAGAAC TAAAGTTAAA AACAGAACCA 
GAAGAAGGTT TAAAACTGAA AGCTGAGGAC CTTGATGCAT TGAAGATGAA AAGGAGAAAA 
GGTTCGTTTG GAAGTATAGA CCAACTCCAG AAATTGGATG AGCAAAAGAA ATGGTTAGAT 
GAAGAAGTAG AAAAAGTTCT GAACCAACGC CAAGAATTAG AGGAGCTGGA AGAAGACTTA 
AAGAAACGGG AGGCTATAGT TTCTAAAAAG GAGGCCCTGT TACAGGAGAA GAGCCACCTG 
GAGAATAAGA AGTTGAGATC TAGTCAGGCC TTAAACACAG ATAGTTTGAA AATATCAACT 
CGCCTGAACT TGCTGGACCA AGAGTTGTCT GAAAAGAATG TACAGCTCCA GAGCAGCACA 
GCTGAGGAGA AAATAAAGAT TTCAGAACAA GTCCAAGCCC TCCAGAAAGA AAAAGATCAG 
CTACAGAGAA GAAGAAACAG TGTGGATGAG AAACTTAAAA ATGGTAGCGT GTTATCACCT 
GAAGAAGAAC ATATTCTTTT CCAACTTGAA GAAGGGATTG AAGCTTTGGA AGCTGCAATT 
GAATACAAGA ATGAAAGTAT CCAGAGTCGC CAGAACTCGC TCAGAGCTTC ATTCCAAAAC 
CTCTCTCATA GTGAAGCAAA TGTCTTGGAA AAACTCATTG GCCTGAATCC TATTGAGATT 
AGAGCTATTC TTTTCAGATA CTTCAATAAG GTGGTTAATT TGCGAGAAGC TGAACGGAAA 
CTACAGTTAC AGAATAAAGA AATTAAAATG AAGATTCTGG AACGGGATAA TGTGGTTCGT 
GAGTTAGAAT CTGCACTGGA GCATCTGAAA TTGCAATGTG ACCGAAGACT GACCCTCCAG 
CAAAAGGAAC ATGAGCAAAA AATGCAGTTA CTATTACATC ATTTCCAAGA GCAAGATGGG 
GAAGGCATCG TGGAAACTTT AAAGACATAT GAAGATAAAA TCCAACAGCT AGAAAAAGAT 
CTTTATTTCT ATAAGAAAAC TAGCAGAGAT CTTAAGAAGA AACTTAAGGA ACTGTTAGGG 
GAAGCAATTC GAAGGCAACT AGTACCATCT GAACATCATT ATGTTGGAGA TGGAGTCCTG 
AACCCAGAAG AAGCAGGCAC GGTTTCAGAA GAATTAAAAT GGGCATCTAA AACTGAAAGT 
ATGAAGTTAA GTGGAAAAGA AAGAGAATTA AACAGTTCAG CAAGTAGCTT AAGAACACAA 
CGAAATCCTC AGAAGCTCTG GGAAGATGGC CCAGAATTAC CTCCAATTTA TAGCTCTTTA 
GCACCCACTA GTGGGCATTT GTTAAGCAAT GAGGATAAAA CAGAAGCAGA TGAAAATCAG 
ATTACAAAAA CTCACAGTTG CACATCACCC CAAATTCAGC CAGTGGGAAA TGTGGGACAG 
ATTCATGGTG TCACACCTGT AAAGTTGTGT CGCAAAGAGT TACGTCAGAT TTCTGCCGTG 
GAACTCTCAT TACGACGTTC CAGTCTTGGA GTTGGGGTTG GATCTATGGC TGCTGATTCC 
ATTGAAGTAG CTAGAAAACA GAGTGACTTA AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023393524.1
CDS	5..4123
Translation	MGRGLTPRGGAWAVGLPGGSRRVGAGLCRLPGEPVTTKGADGSGGGGVAEFSGGVLRCARRRRLGFSLAASVAEGQKGIIPRAIQEIFQNISENLSIDFTIKVSYIEVYKEDLRDLLELETSMKDLHIREDEKGNTVIVGAKECQVESADEVMSFLEMGNAARHTGTTQMNEHSSRSHAIFTISICQVEKNTETVEDGSWCSHRRIVSKFHFVDLAGSERVTKTGNTGERFKESIQINSGLLALGNVISALGDPRRKSSHIPYRDAKITRLLKDSLGGSAKTVMITCVSPSSSDFDESLNSLKYANRARNIRNKPTLNFSPESDRMDEMEFEIKLLREALQSQQASSISQSSQIHREGTPDKNKIHCLEEQVAQLQGECLGYQNCIEEAFTFLVELKDAVRLNQKQQHKLQEWLNMTQEVRKAILTSFRGSRGMGNLEEGPQRSTVLQLKRELKKCQCALAADEVVFNQKELEVKELKNQVQMMVQENKGHVISLKEAQKVNRLQNEKIIEQQLLMDQLNEELTKLNLSMTSSVKETCGDGPDARIPEKRPYTVPFDTHLGHYIYVPARSDARKVYTSPAAYSLDRIVAGFRTRSQMLLGHIEDQDEVIHCQFSDNSDEEESEGQEKSDIRTRSHSWIQKSGSLCSLVELNDIHDQKQKSSLGNEDLKIECLQESQELNLQKLRNSELILNEAKQKMRELTINIKMKEDLIKELMKTGNDAKSVSKQYSLKVTKLAHEAEQAKVELIETEKQLQELENKDLSDVALKVKLQREFRKKMDAAKLKVQVLQKKQQNSKKLASLSIQNEKRANELEQSVDHMKYQKVQLQKRLREENEKRKQLDTEIKRDQQKIKELKLKTEPEEGLKLKAEDLDALKMKRRKGSFGSIDQLQKLDEQKKWLDEEVEKVLNQRQELEELEEDLKKREAIVSKKEALLQEKSHLENKKLRSSQALNTDSLKISTRLNLLDQELSEKNVQLQSSTAEEKIKISEQVQALQKEKDQLQRRRNSVDEKLKNGSVLSPEEEHILFQLEEGIEALEAAIEYKNESIQSRQNSLRASFQNLSHSEANVLEKLIGLNPIEIRAILFRYFNKVVNLREAERKLQLQNKEIKMKILERDNVVRELESALEHLKLQCDRRLTLQQKEHEQKMQLLLHHFQEQDGEGIVETLKTYEDKIQQLEKDLYFYKKTSRDLKKKLKELLGEAIRRQLVPSEHHYVGDGVLNPEEAGTVSEELKWASKTESMKLSGKERELNSSASSLRTQRNPQKLWEDGPELPPIYSSLAPTSGHLLSNEDKTEADENQITKTHSCTSPQIQPVGNVGQIHGVTPVKLCRKELRQISAVELSLRRSSLGVGVGSMAADSIEVARKQSDLKT

Target ORF information:

RefSeq Version	XM_023537756.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023537756.1

ORF Insert Sequence:

ATGGGGCGGG GCTTAACGCC AAGGGGCGGG GCTTGGGCGG TCGGACTCCC AGGCGGGAGC 
CGGCGCGTTG GCGCAGGGCT TTGCCGGTTG CCGGGAGAAC CCGTGACAAC AAAAGGCGCG 
GACGGCAGCG GCGGCGGCGG CGTGGCGGAG TTTAGCGGAG GAGTCCTGCG CTGCGCCCGC 
CGCCGGAGAC TGGGTTTTTC TTTAGCGGCC TCAGTTGCGG AAGGTCAAAA GGGTATTATT 
CCTAGAGCTA TCCAAGAAAT ATTTCAAAAC ATCTCTGAAA ACCTTAGCAT TGACTTTACA 
ATAAAAGTGT CTTATATAGA AGTATATAAA GAAGACTTAA GAGATCTTTT AGAATTGGAG 
ACATCTATGA AGGACCTTCA CATACGAGAA GATGAAAAAG GAAACACAGT GATTGTTGGG 
GCCAAGGAGT GCCAGGTGGA GAGTGCAGAT GAAGTGATGA GCTTCTTGGA GATGGGGAAT 
GCAGCCAGGC ATACAGGTAC CACCCAAATG AACGAACACT CCAGTAGGTC ACATGCAATT 
TTTACAATCA GCATTTGTCA AGTTGAGAAA AATACAGAGA CAGTTGAAGA TGGATCATGG 
TGTTCCCATC GACGTATTGT CTCAAAGTTC CACTTTGTAG ATTTGGCTGG ATCAGAAAGA 
GTAACCAAAA CGGGTAACAC TGGTGAACGG TTCAAAGAAT CCATTCAAAT CAATAGTGGG 
TTGCTGGCTT TAGGAAACGT AATAAGTGCT CTTGGGGACC CACGCAGGAA GAGTTCACAT 
ATTCCGTATA GGGATGCTAA AATTACCCGA CTACTTAAAG ATTCCCTGGG AGGCAGTGCC 
AAGACTGTCA TGATCACTTG TGTCAGCCCA TCCTCCTCGG ATTTTGACGA ATCTTTAAAT 
TCTCTCAAGT ACGCCAACAG AGCACGCAAC ATCAGAAACA AACCCACCTT AAACTTCAGC 
CCGGAGTCAG ATCGGATGGA CGAAATGGAA TTTGAGATTA AGTTGCTTCG AGAGGCTTTG 
CAAAGCCAGC AGGCTAGTAG TATCAGCCAA AGCAGCCAGA TCCATCGAGA GGGGACTCCT 
GATAAAAATA AGATCCATTG TCTTGAGGAG CAAGTAGCTC AGCTCCAAGG AGAATGTCTG 
GGTTACCAAA ACTGTATCGA GGAAGCCTTC ACCTTCCTAG TGGAGTTAAA AGATGCTGTT 
AGACTAAATC AGAAGCAGCA ACACAAACTG CAAGAGTGGC TTAATATGAC CCAGGAGGTC 
AGGAAGGCCA TTCTCACTTC GTTCAGAGGA AGCCGGGGCA TGGGAAATTT GGAAGAAGGA 
CCACAGCGTA GCACAGTTCT CCAGCTGAAA CGAGAGCTGA AGAAATGCCA GTGTGCTCTC 
GCTGCTGATG AAGTAGTATT TAATCAGAAG GAACTGGAGG TGAAGGAACT AAAGAATCAA 
GTGCAAATGA TGGTACAGGA AAACAAAGGA CATGTTATAT CTTTGAAAGA AGCGCAAAAA 
GTTAATAGAT TACAGAATGA AAAAATAATA GAACAACAAC TTCTTATGGA TCAGCTGAAT 
GAAGAACTAA CAAAACTTAA CCTGTCAATG ACTTCTTCAG TTAAGGAAAC TTGTGGAGAT 
GGGCCAGATG CCAGGATACC TGAAAAGAGA CCATATACTG TACCATTTGA TACTCACCTG 
GGGCATTATA TTTATGTCCC AGCAAGATCA GATGCCAGGA AGGTCTACAC AAGTCCTGCT 
GCGTACTCTC TGGATCGCAT AGTTGCTGGA TTCCGAACAC GAAGTCAGAT GCTGCTGGGT 
CATATAGAAG ACCAAGATGA AGTCATCCAC TGCCAGTTTT CTGATAACAG TGATGAGGAA 
GAATCAGAGG GCCAAGAGAA ATCTGATATT AGAACTAGAA GTCACTCATG GATTCAAAAG 
TCTGGCTCCC TTTGTTCTCT TGTTGAACTG AATGATATTC ATGATCAAAA ACAAAAGTCA 
AGTTTGGGGA ATGAAGATTT AAAGATTGAA TGTCTCCAGG AGAGTCAAGA GTTGAATTTG 
CAAAAATTAA GGAATTCAGA ACTCATACTT AATGAAGCAA AACAAAAAAT GAGAGAACTT 
ACAATTAACA TCAAGATGAA AGAAGATCTA ATTAAAGAAT TAATGAAAAC AGGTAATGAT 
GCCAAGTCTG TAAGCAAACA GTATTCTCTG AAAGTAACAA AACTTGCACA TGAAGCAGAA 
CAGGCAAAAG TGGAATTAAT TGAAACAGAA AAACAACTAC AGGAACTGGA AAATAAAGAT 
CTTTCTGATG TTGCTTTGAA AGTAAAATTA CAGAGAGAGT TCCGTAAAAA GATGGATGCC 
GCAAAGCTAA AAGTCCAGGT CTTACAGAAG AAGCAACAAA ATAGTAAGAA ATTGGCATCG 
CTTTCGATCC AAAATGAGAA ACGTGCTAAT GAACTAGAGC AGAGTGTAGA TCACATGAAA 
TATCAAAAGG TACAGCTGCA AAAAAGACTT CGAGAAGAAA ATGAAAAAAG GAAGCAACTG 
GATACAGAAA TTAAGCGGGA TCAACAAAAA ATCAAAGAAC TAAAGTTAAA AACAGAACCA 
GAAGAAGGTT TAAAACTGAA AGCTGAGGAC CTTGATGCAT TGAAGATGAA AAGGAGAAAA 
GGTTCGTTTG GAAGTATAGA CCAACTCCAG AAATTGGATG AGCAAAAGAA ATGGTTAGAT 
GAAGAAGTAG AAAAAGTTCT GAACCAACGC CAAGAATTAG AGGAGCTGGA AGAAGACTTA 
AAGAAACGGG AGGCTATAGT TTCTAAAAAG GAGGCCCTGT TACAGGAGAA GAGCCACCTG 
GAGAATAAGA AGTTGAGATC TAGTCAGGCC TTAAACACAG ATAGTTTGAA AATATCAACT 
CGCCTGAACT TGCTGGACCA AGAGTTGTCT GAAAAGAATG TACAGCTCCA GAGCAGCACA 
GCTGAGGAGA AAATAAAGAT TTCAGAACAA GTCCAAGCCC TCCAGAAAGA AAAAGATCAG 
CTACAGAGAA GAAGAAACAG TGTGGATGAG AAACTTAAAA ATGGTAGCGT GTTATCACCT 
GAAGAAGAAC ATATTCTTTT CCAACTTGAA GAAGGGATTG AAGCTTTGGA AGCTGCAATT 
GAATACAAGA ATGAAAGTAT CCAGAGTCGC CAGAACTCGC TCAGAGCTTC ATTCCAAAAC 
CTCTCTCATA GTGAAGCAAA TGTCTTGGAA AAACTCATTG GCCTGAATCC TATTGAGATT 
AGAGCTATTC TTTTCAGATA CTTCAATAAG GTGGTTAATT TGCGAGAAGC TGAACGGAAA 
CTACAGTTAC AGAATAAAGA AATTAAAATG AAGATTCTGG AACGGGATAA TGTGGTTCGT 
GAGTTAGAAT CTGCACTGGA GCATCTGAAA TTGCAATGTG ACCGAAGACT GACCCTCCAG 
CAAAAGGAAC ATGAGCAAAA AATGCAGTTA CTATTACATC ATTTCCAAGA GCAAGATGGG 
GAAGGCATCG TGGAAACTTT AAAGACATAT GAAGATAAAA TCCAACAGCT AGAAAAAGAT 
CTTTATTTCT ATAAGAAAAC TAGCAGAGAT CTTAAGAAGA AACTTAAGGA ACTGTTAGGG 
GAAGCAATTC GAAGGCAACT AGTACCATCT GAACATCATT ATGTTGGAGA TGGAGTCCTG 
AACCCAGAAG AAGCAGGCAC GGTTTCAGAA GAATTAAAAT GGGCATCTAA AACTGAAAGT 
ATGAAGTTAA GTGGAAAAGA AAGAGAATTA AACAGTTCAG CAAGTAGCTT AAGAACACAA 
CGAAATCCTC AGAAGCTCTG GGAAGATGGC CCAGAATTAC CTCCAATTTA TAGCTCTTTA 
GCACCCACTA GTGGGCATTT GTTAAGCAAT GAGGATAAAA CAGAAGCAGA TGAAAATCAG 
ATTACAAAAA CTCACAGTTG CACATCACCC CAAATTCAGC CAGTGGGAAA TGTGGGACAG 
ATTCATGGTG TCACACCTGT AAAGTTGTGT CGCAAAGAGT TACGTCAGAT TTCTGCCGTG 
GAACTCTCAT TACGACGTTC CAGTCTTGGA GTTGGGGTTG GATCTATGGC TGCTGATTCC 
ATTGAAGTAG CTAGAAAACA GAGTGACTTA AAAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF27 ( XM_023537757.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023393525.1 Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPt73950
Clone ID Related Accession (Same CDS sequence)	XM_023537757.1
Accession Version	XM_023537757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	kinesin-like protein KIF27 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888980.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA TACCAGTAAA AGTTGCTGTA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
CTTCATAATC ATCAAGTTTG TGTGAGAGTT ATTCCAAACA CCCAGCAAAT TATTATTGGG 
AGAGATAGAA TCTTTACTTT CGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT 
TATAATATGT GCATAAAGCC ACTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT 
TTTGCCTACG GTCAAACTGG ATCTGGGAAG ACATATACCA TTGGAGGAGG CCATGTTGCC 
TCAGTTGCGG AAGGTCAAAA GGGTATTATT CCTAGAGCTA TCCAAGAAAT ATTTCAAAAC 
ATCTCTGAAA ACCTTAGCAT TGACTTTACA ATAAAAGTGT CTTATATAGA AGTATATAAA 
GAAGACTTAA GAGATCTTTT AGAATTGGAG ACATCTATGA AGGACCTTCA CATACGAGAA 
GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAGT GCCAGGTGGA GAGTGCAGAT 
GAAGTGATGA GCTTCTTGGA GATGGGGAAT GCAGCCAGGC ATACAGGTAC CACCCAAATG 
AACGAACACT CCAGTAGGTC ACATGCAATT TTTACAATCA GCATTTGTCA AGTTGAGAAA 
AATACAGAGA CAGTTGAAGA TGGATCATGG TGTTCCCATC GACGTATTGT CTCAAAGTTC 
CACTTTGTAG ATTTGGCTGG ATCAGAAAGA GTAACCAAAA CGGGTAACAC TGGTGAACGG 
TTCAAAGAAT CCATTCAAAT CAATAGTGGG TTGCTGGCTT TAGGAAACGT AATAAGTGCT 
CTTGGGGACC CACGCAGGAA GAGTTCACAT ATTCCGTATA GGGATGCTAA AATTACCCGA 
CTACTTAAAG ATTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA 
TCCTCCTCGG ATTTTGACGA ATCTTTAAAT TCTCTCAAGT ACGCCAACAG AGCACGCAAC 
ATCAGAAACA AACCCACCTT AAACTTCAGC CCGGAGTCAG ATCGGATGGA CGAAATGGAA 
TTTGAGATTA AGTTGCTTCG AGAGGCTTTG CAAAGCCAGC AGGCTAGTAG TATCAGCCAA 
AGCAGCCAGA TCCATCGAGA GGGGACTCCT GATAAAAATA AGATCCATTG TCTTGAGGAG 
CAAGTAGCTC AGCTCCAAGG AGAATGTCTG GGTTACCAAA ACTGTATCGA GGAAGCCTTC 
ACCTTCCTAG TGGAGTTAAA AGATGCTGTT AGACTAAATC AGAAGCAGCA ACACAAACTG 
CAAGAGTGGC TTAATATGAC CCAGGAGGTC AGGAAGGCCA TTCTCACTTC GTTCAGAGGA 
AGCCGGGGCA TGGGAAATTT GGAAGAAGGA CCACAGCGTA GCACAGTTCT CCAGCTGAAA 
CGAGAGCTGA AGAAATGCCA GAATGAAAAA ATAATAGAAC AACAACTTCT TATGGATCAG 
CTGAATGAAG AACTAACAAA ACTTAACCTG TCAATGACTT CTTCAGTTAA GGAAACTTGT 
GGAGATGGGC CAGATGCCAG GATACCTGAA AAGAGACCAT ATACTGTACC ATTTGATACT 
CACCTGGGGC ATTATATTTA TGTCCCAGCA AGATCAGATG CCAGGAAGGT CTACACAAGT 
CCTGCTGCGT ACTCTCTGGA TCGCATAGTT GCTGGATTCC GAACACGAAG TCAGATGCTG 
CTGGGTCATA TAGAAGACCA AGATGAAGTC ATCCACTGCC AGTTTTCTGA TAACAGTGAT 
GAGGAAGAAT CAGAGGGCCA AGAGAAATCT GATATTAGAA CTAGAAGTCA CTCATGGATT 
CAAAAGTCTG GCTCCCTTTG TTCTCTTGTT GAACTGAATG ATATTCATGA TCAAAAACAA 
AAGTCAAGTT TGGGGAATGA AGATTTAAAG ATTGAATGTC TCCAGGAGAG TCAAGAGTTG 
AATTTGCAAA AATTAAGGAA TTCAGAACTC ATACTTAATG AAGCAAAACA AAAAATGAGA 
GAACTTACAA TTAACATCAA GATGAAAGAA GATCTAATTA AAGAATTAAT GAAAACAGGT 
AATGATGCCA AGTCTGTAAG CAAACAGTAT TCTCTGAAAG TAACAAAACT TGCACATGAA 
GCAGAACAGG CAAAAGTGGA ATTAATTGAA ACAGAAAAAC AACTACAGGA ACTGGAAAAT 
AAAGATCTTT CTGATGTTGC TTTGAAAGTA AAATTACAGA GAGAGTTCCG TAAAAAGATG 
GATGCCGCAA AGCTAAAAGT CCAGGTCTTA CAGAAGAAGC AACAAAATAG TAAGAAATTG 
GCATCGCTTT CGATCCAAAA TGAGAAACGT GCTAATGAAC TAGAGCAGAG TGTAGATCAC 
ATGAAATATC AAAAGGTACA GCTGCAAAAA AGACTTCGAG AAGAAAATGA AAAAAGGAAG 
CAACTGGATA CAGAAATTAA GCGGGATCAA CAAAAAATCA AAGAACTAAA GTTAAAAACA 
GAACCAGAAG AAGGTTTAAA ACTGAAAGCT GAGGACCTTG ATGCATTGAA GATGAAAAGG 
AGAAAAGGTT CGTTTGGAAG TATAGACCAA CTCCAGAAAT TGGATGAGCA AAAGAAATGG 
TTAGATGAAG AAGTAGAAAA AGTTCTGAAC CAACGCCAAG AATTAGAGGA GCTGGAAGAA 
GACTTAAAGA AACGGGAGGC TATAGTTTCT AAAAAGGAGG CCCTGTTACA GGAGAAGAGC 
CACCTGGAGA ATAAGAAGTT GAGATCTAGT CAGGCCTTAA ACACAGATAG TTTGAAAATA 
TCAACTCGCC TGAACTTGCT GGACCAAGAG TTGTCTGAAA AGAATGTACA GCTCCAGAGC 
AGCACAGCTG AGGAGAAAAT AAAGATTTCA GAACAAGTCC AAGCCCTCCA GAAAGAAAAA 
GATCAGCTAC AGAGAAGAAG AAACAGTGTG GATGAGAAAC TTAAAAATGG TAGCGTGTTA 
TCACCTGAAG AAGAACATAT TCTTTTCCAA CTTGAAGAAG GGATTGAAGC TTTGGAAGCT 
GCAATTGAAT ACAAGAATGA AAGTATCCAG AGTCGCCAGA ACTCGCTCAG AGCTTCATTC 
CAAAACCTCT CTCATAGTGA AGCAAATGTC TTGGAAAAAC TCATTGGCCT GAATCCTATT 
GAGATTAGAG CTATTCTTTT CAGATACTTC AATAAGGTGG TTAATTTGCG AGAAGCTGAA 
CGGAAACTAC AGTTACAGAA TAAAGAAATT AAAATGAAGA TTCTGGAACG GGATAATGTG 
GTTCGTGAGT TAGAATCTGC ACTGGAGCAT CTGAAATTGC AATGTGACCG AAGACTGACC 
CTCCAGCAAA AGGAACATGA GCAAAAAATG CAGTTACTAT TACATCATTT CCAAGAGCAA 
GATGGGGAAG GCATCGTGGA AACTTTAAAG ACATATGAAG ATAAAATCCA ACAGCTAGAA 
AAAGATCTTT ATTTCTATAA GAAAACTAGC AGAGATCTTA AGAAGAAACT TAAGGAACTG 
TTAGGGGAAG CAATTCGAAG GCAACTAGTA CCATCTGAAC ATCATTATGT TGGAGATGGA 
GTCCTGAACC CAGAAGAAGC AGGCACGGTT TCAGAAGAAT TAAAATGGGC ATCTAAAACT 
GAAAGTATGA AGTTAAGTGG AAAAGAAAGA GAATTAAACA GTTCAGCAAG TAGCTTAAGA 
ACACAACGAA ATCCTCAGAA GCTCTGGGAA GATGGCCCAG AATTACCTCC AATTTATAGC 
TCTTTAGCAC CCACTAGTGG GCATTTGTTA AGCAATGAGG ATAAAACAGA AGCAGATGAA 
AATCAGATTA CAAAAACTCA CAGTTGCACA TCACCCCAAA TTCAGCCAGT GGGAAATGTG 
GGACAGATTC ATGGTGTCAC ACCTGTAAAG TTGTGTCGCA AAGAGTTACG TCAGATTTCT 
GCCGTGGAAC TCTCATTACG ACGTTCCAGT CTTGGAGTTG GGGTTGGATC TATGGCTGCT 
GATTCCATTG AAGTAGCTAG AAAACAGAGT GACTTAAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023393525.1
CDS	226..4290
Translation	MEEIPVKVAVRIRPLLCKEVLHNHQVCVRVIPNTQQIIIGRDRIFTFDFVFGKNSTQDEVYNMCIKPLVLSLIEGYNATVFAYGQTGSGKTYTIGGGHVASVAEGQKGIIPRAIQEIFQNISENLSIDFTIKVSYIEVYKEDLRDLLELETSMKDLHIREDEKGNTVIVGAKECQVESADEVMSFLEMGNAARHTGTTQMNEHSSRSHAIFTISICQVEKNTETVEDGSWCSHRRIVSKFHFVDLAGSERVTKTGNTGERFKESIQINSGLLALGNVISALGDPRRKSSHIPYRDAKITRLLKDSLGGSAKTVMITCVSPSSSDFDESLNSLKYANRARNIRNKPTLNFSPESDRMDEMEFEIKLLREALQSQQASSISQSSQIHREGTPDKNKIHCLEEQVAQLQGECLGYQNCIEEAFTFLVELKDAVRLNQKQQHKLQEWLNMTQEVRKAILTSFRGSRGMGNLEEGPQRSTVLQLKRELKKCQNEKIIEQQLLMDQLNEELTKLNLSMTSSVKETCGDGPDARIPEKRPYTVPFDTHLGHYIYVPARSDARKVYTSPAAYSLDRIVAGFRTRSQMLLGHIEDQDEVIHCQFSDNSDEEESEGQEKSDIRTRSHSWIQKSGSLCSLVELNDIHDQKQKSSLGNEDLKIECLQESQELNLQKLRNSELILNEAKQKMRELTINIKMKEDLIKELMKTGNDAKSVSKQYSLKVTKLAHEAEQAKVELIETEKQLQELENKDLSDVALKVKLQREFRKKMDAAKLKVQVLQKKQQNSKKLASLSIQNEKRANELEQSVDHMKYQKVQLQKRLREENEKRKQLDTEIKRDQQKIKELKLKTEPEEGLKLKAEDLDALKMKRRKGSFGSIDQLQKLDEQKKWLDEEVEKVLNQRQELEELEEDLKKREAIVSKKEALLQEKSHLENKKLRSSQALNTDSLKISTRLNLLDQELSEKNVQLQSSTAEEKIKISEQVQALQKEKDQLQRRRNSVDEKLKNGSVLSPEEEHILFQLEEGIEALEAAIEYKNESIQSRQNSLRASFQNLSHSEANVLEKLIGLNPIEIRAILFRYFNKVVNLREAERKLQLQNKEIKMKILERDNVVRELESALEHLKLQCDRRLTLQQKEHEQKMQLLLHHFQEQDGEGIVETLKTYEDKIQQLEKDLYFYKKTSRDLKKKLKELLGEAIRRQLVPSEHHYVGDGVLNPEEAGTVSEELKWASKTESMKLSGKERELNSSASSLRTQRNPQKLWEDGPELPPIYSSLAPTSGHLLSNEDKTEADENQITKTHSCTSPQIQPVGNVGQIHGVTPVKLCRKELRQISAVELSLRRSSLGVGVGSMAADSIEVARKQSDLKT

Target ORF information:

RefSeq Version	XM_023537757.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023537757.1

ORF Insert Sequence:

ATGGAAGAAA TACCAGTAAA AGTTGCTGTA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
CTTCATAATC ATCAAGTTTG TGTGAGAGTT ATTCCAAACA CCCAGCAAAT TATTATTGGG 
AGAGATAGAA TCTTTACTTT CGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT 
TATAATATGT GCATAAAGCC ACTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT 
TTTGCCTACG GTCAAACTGG ATCTGGGAAG ACATATACCA TTGGAGGAGG CCATGTTGCC 
TCAGTTGCGG AAGGTCAAAA GGGTATTATT CCTAGAGCTA TCCAAGAAAT ATTTCAAAAC 
ATCTCTGAAA ACCTTAGCAT TGACTTTACA ATAAAAGTGT CTTATATAGA AGTATATAAA 
GAAGACTTAA GAGATCTTTT AGAATTGGAG ACATCTATGA AGGACCTTCA CATACGAGAA 
GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAGT GCCAGGTGGA GAGTGCAGAT 
GAAGTGATGA GCTTCTTGGA GATGGGGAAT GCAGCCAGGC ATACAGGTAC CACCCAAATG 
AACGAACACT CCAGTAGGTC ACATGCAATT TTTACAATCA GCATTTGTCA AGTTGAGAAA 
AATACAGAGA CAGTTGAAGA TGGATCATGG TGTTCCCATC GACGTATTGT CTCAAAGTTC 
CACTTTGTAG ATTTGGCTGG ATCAGAAAGA GTAACCAAAA CGGGTAACAC TGGTGAACGG 
TTCAAAGAAT CCATTCAAAT CAATAGTGGG TTGCTGGCTT TAGGAAACGT AATAAGTGCT 
CTTGGGGACC CACGCAGGAA GAGTTCACAT ATTCCGTATA GGGATGCTAA AATTACCCGA 
CTACTTAAAG ATTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA 
TCCTCCTCGG ATTTTGACGA ATCTTTAAAT TCTCTCAAGT ACGCCAACAG AGCACGCAAC 
ATCAGAAACA AACCCACCTT AAACTTCAGC CCGGAGTCAG ATCGGATGGA CGAAATGGAA 
TTTGAGATTA AGTTGCTTCG AGAGGCTTTG CAAAGCCAGC AGGCTAGTAG TATCAGCCAA 
AGCAGCCAGA TCCATCGAGA GGGGACTCCT GATAAAAATA AGATCCATTG TCTTGAGGAG 
CAAGTAGCTC AGCTCCAAGG AGAATGTCTG GGTTACCAAA ACTGTATCGA GGAAGCCTTC 
ACCTTCCTAG TGGAGTTAAA AGATGCTGTT AGACTAAATC AGAAGCAGCA ACACAAACTG 
CAAGAGTGGC TTAATATGAC CCAGGAGGTC AGGAAGGCCA TTCTCACTTC GTTCAGAGGA 
AGCCGGGGCA TGGGAAATTT GGAAGAAGGA CCACAGCGTA GCACAGTTCT CCAGCTGAAA 
CGAGAGCTGA AGAAATGCCA GAATGAAAAA ATAATAGAAC AACAACTTCT TATGGATCAG 
CTGAATGAAG AACTAACAAA ACTTAACCTG TCAATGACTT CTTCAGTTAA GGAAACTTGT 
GGAGATGGGC CAGATGCCAG GATACCTGAA AAGAGACCAT ATACTGTACC ATTTGATACT 
CACCTGGGGC ATTATATTTA TGTCCCAGCA AGATCAGATG CCAGGAAGGT CTACACAAGT 
CCTGCTGCGT ACTCTCTGGA TCGCATAGTT GCTGGATTCC GAACACGAAG TCAGATGCTG 
CTGGGTCATA TAGAAGACCA AGATGAAGTC ATCCACTGCC AGTTTTCTGA TAACAGTGAT 
GAGGAAGAAT CAGAGGGCCA AGAGAAATCT GATATTAGAA CTAGAAGTCA CTCATGGATT 
CAAAAGTCTG GCTCCCTTTG TTCTCTTGTT GAACTGAATG ATATTCATGA TCAAAAACAA 
AAGTCAAGTT TGGGGAATGA AGATTTAAAG ATTGAATGTC TCCAGGAGAG TCAAGAGTTG 
AATTTGCAAA AATTAAGGAA TTCAGAACTC ATACTTAATG AAGCAAAACA AAAAATGAGA 
GAACTTACAA TTAACATCAA GATGAAAGAA GATCTAATTA AAGAATTAAT GAAAACAGGT 
AATGATGCCA AGTCTGTAAG CAAACAGTAT TCTCTGAAAG TAACAAAACT TGCACATGAA 
GCAGAACAGG CAAAAGTGGA ATTAATTGAA ACAGAAAAAC AACTACAGGA ACTGGAAAAT 
AAAGATCTTT CTGATGTTGC TTTGAAAGTA AAATTACAGA GAGAGTTCCG TAAAAAGATG 
GATGCCGCAA AGCTAAAAGT CCAGGTCTTA CAGAAGAAGC AACAAAATAG TAAGAAATTG 
GCATCGCTTT CGATCCAAAA TGAGAAACGT GCTAATGAAC TAGAGCAGAG TGTAGATCAC 
ATGAAATATC AAAAGGTACA GCTGCAAAAA AGACTTCGAG AAGAAAATGA AAAAAGGAAG 
CAACTGGATA CAGAAATTAA GCGGGATCAA CAAAAAATCA AAGAACTAAA GTTAAAAACA 
GAACCAGAAG AAGGTTTAAA ACTGAAAGCT GAGGACCTTG ATGCATTGAA GATGAAAAGG 
AGAAAAGGTT CGTTTGGAAG TATAGACCAA CTCCAGAAAT TGGATGAGCA AAAGAAATGG 
TTAGATGAAG AAGTAGAAAA AGTTCTGAAC CAACGCCAAG AATTAGAGGA GCTGGAAGAA 
GACTTAAAGA AACGGGAGGC TATAGTTTCT AAAAAGGAGG CCCTGTTACA GGAGAAGAGC 
CACCTGGAGA ATAAGAAGTT GAGATCTAGT CAGGCCTTAA ACACAGATAG TTTGAAAATA 
TCAACTCGCC TGAACTTGCT GGACCAAGAG TTGTCTGAAA AGAATGTACA GCTCCAGAGC 
AGCACAGCTG AGGAGAAAAT AAAGATTTCA GAACAAGTCC AAGCCCTCCA GAAAGAAAAA 
GATCAGCTAC AGAGAAGAAG AAACAGTGTG GATGAGAAAC TTAAAAATGG TAGCGTGTTA 
TCACCTGAAG AAGAACATAT TCTTTTCCAA CTTGAAGAAG GGATTGAAGC TTTGGAAGCT 
GCAATTGAAT ACAAGAATGA AAGTATCCAG AGTCGCCAGA ACTCGCTCAG AGCTTCATTC 
CAAAACCTCT CTCATAGTGA AGCAAATGTC TTGGAAAAAC TCATTGGCCT GAATCCTATT 
GAGATTAGAG CTATTCTTTT CAGATACTTC AATAAGGTGG TTAATTTGCG AGAAGCTGAA 
CGGAAACTAC AGTTACAGAA TAAAGAAATT AAAATGAAGA TTCTGGAACG GGATAATGTG 
GTTCGTGAGT TAGAATCTGC ACTGGAGCAT CTGAAATTGC AATGTGACCG AAGACTGACC 
CTCCAGCAAA AGGAACATGA GCAAAAAATG CAGTTACTAT TACATCATTT CCAAGAGCAA 
GATGGGGAAG GCATCGTGGA AACTTTAAAG ACATATGAAG ATAAAATCCA ACAGCTAGAA 
AAAGATCTTT ATTTCTATAA GAAAACTAGC AGAGATCTTA AGAAGAAACT TAAGGAACTG 
TTAGGGGAAG CAATTCGAAG GCAACTAGTA CCATCTGAAC ATCATTATGT TGGAGATGGA 
GTCCTGAACC CAGAAGAAGC AGGCACGGTT TCAGAAGAAT TAAAATGGGC ATCTAAAACT 
GAAAGTATGA AGTTAAGTGG AAAAGAAAGA GAATTAAACA GTTCAGCAAG TAGCTTAAGA 
ACACAACGAA ATCCTCAGAA GCTCTGGGAA GATGGCCCAG AATTACCTCC AATTTATAGC 
TCTTTAGCAC CCACTAGTGG GCATTTGTTA AGCAATGAGG ATAAAACAGA AGCAGATGAA 
AATCAGATTA CAAAAACTCA CAGTTGCACA TCACCCCAAA TTCAGCCAGT GGGAAATGTG 
GGACAGATTC ATGGTGTCAC ACCTGTAAAG TTGTGTCGCA AAGAGTTACG TCAGATTTCT 
GCCGTGGAAC TCTCATTACG ACGTTCCAGT CTTGGAGTTG GGGTTGGATC TATGGCTGCT 
GATTCCATTG AAGTAGCTAG AAAACAGAGT GACTTAAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF27 ( XM_023537758.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023393526.1 Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X4, mRNA.

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CloneID	OPt73951
Clone ID Related Accession (Same CDS sequence)	XM_023537758.1
Accession Version	XM_023537758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	kinesin-like protein KIF27 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888980.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAACACAGTA CGCCAACAGA GCACGCAACA TCAGAAACAA ACCCACCTTA 
AACTTCAGCC CGGAGTCAGA TCGGATGGAC GAAATGGAAT TTGAGATTAA GTTGCTTCGA 
GAGGCTTTGC AAAGCCAGCA GGCTAGTAGT ATCAGCCAAA GCAGCCAGAT CCATCGAGAG 
GGGACTCCTG ATAAAAATAA GATCCATTGT CTTGAGGAGC AAGTAGCTCA GCTCCAAGGA 
GAATGTCTGG GTTACCAAAA CTGTATCGAG GAAGCCTTCA CCTTCCTAGT GGAGTTAAAA 
GATGCTGTTA GACTAAATCA GAAGCAGCAA CACAAACTGC AAGAGTGGCT TAATATGACC 
CAGGAGGTCA GGAAGGCCAT TCTCACTTCG TTCAGAGGAA GCCGGGGCAT GGGAAATTTG 
GAAGAAGGAC CACAGCGTAG CACAGTTCTC CAGCTGAAAC GAGAGCTGAA GAAATGCCAG 
TGTGCTCTCG CTGCTGATGA AGTAGTATTT AATCAGAAGG AACTGGAGGT GAAGGAACTA 
AAGAATCAAG TGCAAATGAT GGTACAGGAA AACAAAGGAC ATGTTATATC TTTGAAAGAA 
GCGCAAAAAG TTAATAGATT ACAGAATGAA AAAATAATAG AACAACAACT TCTTATGGAT 
CAGCTGAATG AAGAACTAAC AAAACTTAAC CTGTCAATGA CTTCTTCAGT TAAGGAAACT 
TGTGGAGATG GGCCAGATGC CAGGATACCT GAAAAGAGAC CATATACTGT ACCATTTGAT 
ACTCACCTGG GGCATTATAT TTATGTCCCA GCAAGATCAG ATGCCAGGAA GGTCTACACA 
AGTCCTGCTG CGTACTCTCT GGATCGCATA GTTGCTGGAT TCCGAACACG AAGTCAGATG 
CTGCTGGGTC ATATAGAAGA CCAAGATGAA GTCATCCACT GCCAGTTTTC TGATAACAGT 
GATGAGGAAG AATCAGAGGG CCAAGAGAAA TCTGATATTA GAACTAGAAG TCACTCATGG 
ATTCAAAAGT CTGGCTCCCT TTGTTCTCTT GTTGAACTGA ATGATATTCA TGATCAAAAA 
CAAAAGTCAA GTTTGGGGAA TGAAGATTTA AAGATTGAAT GTCTCCAGGA GAGTCAAGAG 
TTGAATTTGC AAAAATTAAG GAATTCAGAA CTCATACTTA ATGAAGCAAA ACAAAAAATG 
AGAGAACTTA CAATTAACAT CAAGATGAAA GAAGATCTAA TTAAAGAATT AATGAAAACA 
GGTAATGATG CCAAGTCTGT AAGCAAACAG TATTCTCTGA AAGTAACAAA ACTTGCACAT 
GAAGCAGAAC AGGCAAAAGT GGAATTAATT GAAACAGAAA AACAACTACA GGAACTGGAA 
AATAAAGATC TTTCTGATGT TGCTTTGAAA GTAAAATTAC AGAGAGAGTT CCGTAAAAAG 
ATGGATGCCG CAAAGCTAAA AGTCCAGGTC TTACAGAAGA AGCAACAAAA TAGTAAGAAA 
TTGGCATCGC TTTCGATCCA AAATGAGAAA CGTGCTAATG AACTAGAGCA GAGTGTAGAT 
CACATGAAAT ATCAAAAGGT ACAGCTGCAA AAAAGACTTC GAGAAGAAAA TGAAAAAAGG 
AAGCAACTGG ATACAGAAAT TAAGCGGGAT CAACAAAAAA TCAAAGAACT AAAGTTAAAA 
ACAGAACCAG AAGAAGGTTT AAAACTGAAA GCTGAGGACC TTGATGCATT GAAGATGAAA 
AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATGA GCAAAAGAAA 
TGGTTAGATG AAGAAGTAGA AAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA 
GAAGACTTAA AGAAACGGGA GGCTATAGTT TCTAAAAAGG AGGCCCTGTT ACAGGAGAAG 
AGCCACCTGG AGAATAAGAA GTTGAGATCT AGTCAGGCCT TAAACACAGA TAGTTTGAAA 
ATATCAACTC GCCTGAACTT GCTGGACCAA GAGTTGTCTG AAAAGAATGT ACAGCTCCAG 
AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TCCAAGCCCT CCAGAAAGAA 
AAAGATCAGC TACAGAGAAG AAGAAACAGT GTGGATGAGA AACTTAAAAA TGGTAGCGTG 
TTATCACCTG AAGAAGAACA TATTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA 
GCTGCAATTG AATACAAGAA TGAAAGTATC CAGAGTCGCC AGAACTCGCT CAGAGCTTCA 
TTCCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTGGAAA AACTCATTGG CCTGAATCCT 
ATTGAGATTA GAGCTATTCT TTTCAGATAC TTCAATAAGG TGGTTAATTT GCGAGAAGCT 
GAACGGAAAC TACAGTTACA GAATAAAGAA ATTAAAATGA AGATTCTGGA ACGGGATAAT 
GTGGTTCGTG AGTTAGAATC TGCACTGGAG CATCTGAAAT TGCAATGTGA CCGAAGACTG 
ACCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAGTTAC TATTACATCA TTTCCAAGAG 
CAAGATGGGG AAGGCATCGT GGAAACTTTA AAGACATATG AAGATAAAAT CCAACAGCTA 
GAAAAAGATC TTTATTTCTA TAAGAAAACT AGCAGAGATC TTAAGAAGAA ACTTAAGGAA 
CTGTTAGGGG AAGCAATTCG AAGGCAACTA GTACCATCTG AACATCATTA TGTTGGAGAT 
GGAGTCCTGA ACCCAGAAGA AGCAGGCACG GTTTCAGAAG AATTAAAATG GGCATCTAAA 
ACTGAAAGTA TGAAGTTAAG TGGAAAAGAA AGAGAATTAA ACAGTTCAGC AAGTAGCTTA 
AGAACACAAC GAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTACC TCCAATTTAT 
AGCTCTTTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGATAAAAC AGAAGCAGAT 
GAAAATCAGA TTACAAAAAC TCACAGTTGC ACATCACCCC AAATTCAGCC AGTGGGAAAT 
GTGGGACAGA TTCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGTCAGATT 
TCTGCCGTGG AACTCTCATT ACGACGTTCC AGTCTTGGAG TTGGGGTTGG ATCTATGGCT 
GCTGATTCCA TTGAAGTAGC TAGAAAACAG AGTGACTTAA AAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023393526.1
CDS	141..3368
Translation	MKKETQYANRARNIRNKPTLNFSPESDRMDEMEFEIKLLREALQSQQASSISQSSQIHREGTPDKNKIHCLEEQVAQLQGECLGYQNCIEEAFTFLVELKDAVRLNQKQQHKLQEWLNMTQEVRKAILTSFRGSRGMGNLEEGPQRSTVLQLKRELKKCQCALAADEVVFNQKELEVKELKNQVQMMVQENKGHVISLKEAQKVNRLQNEKIIEQQLLMDQLNEELTKLNLSMTSSVKETCGDGPDARIPEKRPYTVPFDTHLGHYIYVPARSDARKVYTSPAAYSLDRIVAGFRTRSQMLLGHIEDQDEVIHCQFSDNSDEEESEGQEKSDIRTRSHSWIQKSGSLCSLVELNDIHDQKQKSSLGNEDLKIECLQESQELNLQKLRNSELILNEAKQKMRELTINIKMKEDLIKELMKTGNDAKSVSKQYSLKVTKLAHEAEQAKVELIETEKQLQELENKDLSDVALKVKLQREFRKKMDAAKLKVQVLQKKQQNSKKLASLSIQNEKRANELEQSVDHMKYQKVQLQKRLREENEKRKQLDTEIKRDQQKIKELKLKTEPEEGLKLKAEDLDALKMKRRKGSFGSIDQLQKLDEQKKWLDEEVEKVLNQRQELEELEEDLKKREAIVSKKEALLQEKSHLENKKLRSSQALNTDSLKISTRLNLLDQELSEKNVQLQSSTAEEKIKISEQVQALQKEKDQLQRRRNSVDEKLKNGSVLSPEEEHILFQLEEGIEALEAAIEYKNESIQSRQNSLRASFQNLSHSEANVLEKLIGLNPIEIRAILFRYFNKVVNLREAERKLQLQNKEIKMKILERDNVVRELESALEHLKLQCDRRLTLQQKEHEQKMQLLLHHFQEQDGEGIVETLKTYEDKIQQLEKDLYFYKKTSRDLKKKLKELLGEAIRRQLVPSEHHYVGDGVLNPEEAGTVSEELKWASKTESMKLSGKERELNSSASSLRTQRNPQKLWEDGPELPPIYSSLAPTSGHLLSNEDKTEADENQITKTHSCTSPQIQPVGNVGQIHGVTPVKLCRKELRQISAVELSLRRSSLGVGVGSMAADSIEVARKQSDLKT

Target ORF information:

RefSeq Version	XM_023537758.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus kinesin family member 27 (KIF27), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023537758.1

ORF Insert Sequence:

ATGAAAAAGG AAACACAGTA CGCCAACAGA GCACGCAACA TCAGAAACAA ACCCACCTTA 
AACTTCAGCC CGGAGTCAGA TCGGATGGAC GAAATGGAAT TTGAGATTAA GTTGCTTCGA 
GAGGCTTTGC AAAGCCAGCA GGCTAGTAGT ATCAGCCAAA GCAGCCAGAT CCATCGAGAG 
GGGACTCCTG ATAAAAATAA GATCCATTGT CTTGAGGAGC AAGTAGCTCA GCTCCAAGGA 
GAATGTCTGG GTTACCAAAA CTGTATCGAG GAAGCCTTCA CCTTCCTAGT GGAGTTAAAA 
GATGCTGTTA GACTAAATCA GAAGCAGCAA CACAAACTGC AAGAGTGGCT TAATATGACC 
CAGGAGGTCA GGAAGGCCAT TCTCACTTCG TTCAGAGGAA GCCGGGGCAT GGGAAATTTG 
GAAGAAGGAC CACAGCGTAG CACAGTTCTC CAGCTGAAAC GAGAGCTGAA GAAATGCCAG 
TGTGCTCTCG CTGCTGATGA AGTAGTATTT AATCAGAAGG AACTGGAGGT GAAGGAACTA 
AAGAATCAAG TGCAAATGAT GGTACAGGAA AACAAAGGAC ATGTTATATC TTTGAAAGAA 
GCGCAAAAAG TTAATAGATT ACAGAATGAA AAAATAATAG AACAACAACT TCTTATGGAT 
CAGCTGAATG AAGAACTAAC AAAACTTAAC CTGTCAATGA CTTCTTCAGT TAAGGAAACT 
TGTGGAGATG GGCCAGATGC CAGGATACCT GAAAAGAGAC CATATACTGT ACCATTTGAT 
ACTCACCTGG GGCATTATAT TTATGTCCCA GCAAGATCAG ATGCCAGGAA GGTCTACACA 
AGTCCTGCTG CGTACTCTCT GGATCGCATA GTTGCTGGAT TCCGAACACG AAGTCAGATG 
CTGCTGGGTC ATATAGAAGA CCAAGATGAA GTCATCCACT GCCAGTTTTC TGATAACAGT 
GATGAGGAAG AATCAGAGGG CCAAGAGAAA TCTGATATTA GAACTAGAAG TCACTCATGG 
ATTCAAAAGT CTGGCTCCCT TTGTTCTCTT GTTGAACTGA ATGATATTCA TGATCAAAAA 
CAAAAGTCAA GTTTGGGGAA TGAAGATTTA AAGATTGAAT GTCTCCAGGA GAGTCAAGAG 
TTGAATTTGC AAAAATTAAG GAATTCAGAA CTCATACTTA ATGAAGCAAA ACAAAAAATG 
AGAGAACTTA CAATTAACAT CAAGATGAAA GAAGATCTAA TTAAAGAATT AATGAAAACA 
GGTAATGATG CCAAGTCTGT AAGCAAACAG TATTCTCTGA AAGTAACAAA ACTTGCACAT 
GAAGCAGAAC AGGCAAAAGT GGAATTAATT GAAACAGAAA AACAACTACA GGAACTGGAA 
AATAAAGATC TTTCTGATGT TGCTTTGAAA GTAAAATTAC AGAGAGAGTT CCGTAAAAAG 
ATGGATGCCG CAAAGCTAAA AGTCCAGGTC TTACAGAAGA AGCAACAAAA TAGTAAGAAA 
TTGGCATCGC TTTCGATCCA AAATGAGAAA CGTGCTAATG AACTAGAGCA GAGTGTAGAT 
CACATGAAAT ATCAAAAGGT ACAGCTGCAA AAAAGACTTC GAGAAGAAAA TGAAAAAAGG 
AAGCAACTGG ATACAGAAAT TAAGCGGGAT CAACAAAAAA TCAAAGAACT AAAGTTAAAA 
ACAGAACCAG AAGAAGGTTT AAAACTGAAA GCTGAGGACC TTGATGCATT GAAGATGAAA 
AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATGA GCAAAAGAAA 
TGGTTAGATG AAGAAGTAGA AAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA 
GAAGACTTAA AGAAACGGGA GGCTATAGTT TCTAAAAAGG AGGCCCTGTT ACAGGAGAAG 
AGCCACCTGG AGAATAAGAA GTTGAGATCT AGTCAGGCCT TAAACACAGA TAGTTTGAAA 
ATATCAACTC GCCTGAACTT GCTGGACCAA GAGTTGTCTG AAAAGAATGT ACAGCTCCAG 
AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TCCAAGCCCT CCAGAAAGAA 
AAAGATCAGC TACAGAGAAG AAGAAACAGT GTGGATGAGA AACTTAAAAA TGGTAGCGTG 
TTATCACCTG AAGAAGAACA TATTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA 
GCTGCAATTG AATACAAGAA TGAAAGTATC CAGAGTCGCC AGAACTCGCT CAGAGCTTCA 
TTCCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTGGAAA AACTCATTGG CCTGAATCCT 
ATTGAGATTA GAGCTATTCT TTTCAGATAC TTCAATAAGG TGGTTAATTT GCGAGAAGCT 
GAACGGAAAC TACAGTTACA GAATAAAGAA ATTAAAATGA AGATTCTGGA ACGGGATAAT 
GTGGTTCGTG AGTTAGAATC TGCACTGGAG CATCTGAAAT TGCAATGTGA CCGAAGACTG 
ACCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAGTTAC TATTACATCA TTTCCAAGAG 
CAAGATGGGG AAGGCATCGT GGAAACTTTA AAGACATATG AAGATAAAAT CCAACAGCTA 
GAAAAAGATC TTTATTTCTA TAAGAAAACT AGCAGAGATC TTAAGAAGAA ACTTAAGGAA 
CTGTTAGGGG AAGCAATTCG AAGGCAACTA GTACCATCTG AACATCATTA TGTTGGAGAT 
GGAGTCCTGA ACCCAGAAGA AGCAGGCACG GTTTCAGAAG AATTAAAATG GGCATCTAAA 
ACTGAAAGTA TGAAGTTAAG TGGAAAAGAA AGAGAATTAA ACAGTTCAGC AAGTAGCTTA 
AGAACACAAC GAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTACC TCCAATTTAT 
AGCTCTTTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGATAAAAC AGAAGCAGAT 
GAAAATCAGA TTACAAAAAC TCACAGTTGC ACATCACCCC AAATTCAGCC AGTGGGAAAT 
GTGGGACAGA TTCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGTCAGATT 
TCTGCCGTGG AACTCTCATT ACGACGTTCC AGTCTTGGAG TTGGGGTTGG ATCTATGGCT 
GCTGATTCCA TTGAAGTAGC TAGAAAACAG AGTGACTTAA AAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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KIF27 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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