SPAG5 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAG5
Entrez Gene ID	105291919
Full Name	sperm associated antigen 5
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011359970.2	XP_011358272.1	sperm-associated antigen 5 isoform X2
XM_011359971.2	XP_011358273.1	sperm-associated antigen 5 isoform X3
XM_011359971.1	XP_011358273.1	sperm-associated antigen 5 isoform X3
XM_011359970.1	XP_011358272.1	sperm-associated antigen 5 isoform X2
XM_011359969.1	XP_011358271.1	sperm-associated antigen 5 isoform X1
XM_011359969.2	XP_011358271.1	sperm-associated antigen 5 isoform X1

Pathways from BioSystems
Gene Ontology

The following SPAG5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAG5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt46672	XM_011359969.2 Latest version!	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46673	XM_011359970.2 Latest version!	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46674	XM_011359971.2 Latest version!	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OPt46674	XM_011359971.1	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46673	XM_011359970.1	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt46672	XM_011359969.1	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAG5 ( XM_011359969.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358271.1 Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X1, mRNA.

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CloneID	OPt46672
Clone ID Related Accession (Same CDS sequence)	XM_011359969.1 , XM_011359969.2
Accession Version	XM_011359969.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	sperm-associated antigen 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888831.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGATGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC TACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGGACTTT CTCTTTCAGA GATCCCAGAT 
ATGGAGAAGA GCCTGGCAGA AATGAGTACT ATCACTCTGG AGCTTCAGAG CCTGTGTTCC 
TTGTTGCAAG GGTCTAAAGA AGAAGCCGTC AGGAGTATGC AACAAAAGAT TTGTGATCTG 
CAGGCTAGGC TGCAGGCCCA GGAAGAACAG CATCAGGAAG CCCTGAAGGC AAAGGAAGCA 
GACATAGAGA AGCTGAACCA GGCCTTGTGC TTGCGCTACA AGAATGAAAA GGAGCTCCAG 
GAAGTGATAC AGCAACAGAA TGAGAAGATT CTAGAACAGA TAGACAAGAG TGGCGAGCTC 
ATAAGTCTTA GAGAGGAGGT GACCCAGCTT ACCCGCTCAC TTCGGCGTGC GGAGACAGAG 
ACTAAGGTGC TCCAAGAGAC CCTGGCAGGT CAGCTGGACC CCAACTCTCA GCCCATGGTT 
ACTAACTGGA TCCAGGAAAA AGTGTGGCTC TCCCAGGAGG TGGACAAACT GAGGGTGATG 
TTCCTGGAAA TGAAAAATGA GAAGGCAAAA CTCACAGTCA AGTTCCAGAG CCATAGAAAT 
ATCCTGGAGG AGAATCTTCG GCGTTCTGAC AAGGAGTTAA AGAAACTAGA CGACATCGTT 
CAGCACATTT ATGAGACTCT GTTGTCCATC CCAGAGGTCG TGAGGGGTTG CAAGGAGCTT 
CAAGGATTGC TGGAATTTCT GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358271.1
CDS	12..3698
Translation	MKTAGEQVVSFWVCQVVVVRSMLKMWRVKKVNLSLSLSPQPGKPAMRTPLRELNLHPDALTSSGKGPPMGYSLTPSLCKLELQECTNNSSLLGFENPKMTDSPSEQCSHPSKWLEACQHESDEQPLNLIPQTNSTPKTSEEAVDPLDNNVIKTMILVPSSPGGQQQDITLKAHLDTMAEINSFSLDKPLRPGDLLRKGETPCMEDSFSRVVPAMPEKPTFRNPPSHLLEYPSNSCSEQQLHCSRENLRDSSSEAVPEDLVASETNAILPSSMLCLCPPTALAADFLVSHVDPGEETVEHRAIEEGEMNFPTLTEEAELGDQARVLNMEDIPSSCLIPNPGEMESQAAPCPAVEDAGRILNSDTGPWMSPLAWLEKGVNTSVMLENLRQSLSFPSVLRDAATGTTPFSTCSVGTWFTPPVPQEKSTNTSQTGLVGTKDNTSEREHLLWGHPPDLTALSRHDLEDNLMNSLVILEVLSHQLQNWKSQLTIPHPEVQDSSTQTDTTDTSPSGISKKSQHLLESQEVGQALLQARNVLQSWALVSKELISLLHLSLLHLEEDKTTVSQESRRAETLISCCFDVLKKLKARLQSLKAEREEAKHREEMALRGKDAAETVLEAFCAHASQRISQLKQDLACMGEFRGLLKETQTQLVGLHTEKEELAQQTVSLISVLQQDWISMQLDYITWTALLSRSRQLTEKLTAKRQQTLQERDTAIEEKQQVSRELERVSTQLEDCKGQIGQLELENSRLAADLQAQLQILASMQSQLEELQSQHAHCTQDLATKDELLCQLTQSNEEQATQWQKEEMALKHMQAKLQQQHAVLAKEVQDLKETLEFADQENQVAHLELGQVECQLKTTLEVLRERSLQCEDLRDTVENLKAQLASTIAENQKKDLEKTRQYSQELRGLTKQLQSLTLFLQTKLKEKAEPETLLKSTACAPAQEHPLSTDSTFLGSISTAVADKEPESPPMSLLGSDKSAFTRVASMASLQPTGLSLSEIPDMEKSLAEMSTITLELQSLCSLLQGSKEEAVRSMQQKICDLQARLQAQEEQHQEALKAKEADIEKLNQALCLRYKNEKELQEVIQQQNEKILEQIDKSGELISLREEVTQLTRSLRRAETETKVLQETLAGQLDPNSQPMVTNWIQEKVWLSQEVDKLRVMFLEMKNEKAKLTVKFQSHRNILEENLRRSDKELKKLDDIVQHIYETLLSIPEVVRGCKELQGLLEFLS

Target ORF information:

RefSeq Version	XM_011359969.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011359969.1

ORF Insert Sequence:

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGATGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC TACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGGACTTT CTCTTTCAGA GATCCCAGAT 
ATGGAGAAGA GCCTGGCAGA AATGAGTACT ATCACTCTGG AGCTTCAGAG CCTGTGTTCC 
TTGTTGCAAG GGTCTAAAGA AGAAGCCGTC AGGAGTATGC AACAAAAGAT TTGTGATCTG 
CAGGCTAGGC TGCAGGCCCA GGAAGAACAG CATCAGGAAG CCCTGAAGGC AAAGGAAGCA 
GACATAGAGA AGCTGAACCA GGCCTTGTGC TTGCGCTACA AGAATGAAAA GGAGCTCCAG 
GAAGTGATAC AGCAACAGAA TGAGAAGATT CTAGAACAGA TAGACAAGAG TGGCGAGCTC 
ATAAGTCTTA GAGAGGAGGT GACCCAGCTT ACCCGCTCAC TTCGGCGTGC GGAGACAGAG 
ACTAAGGTGC TCCAAGAGAC CCTGGCAGGT CAGCTGGACC CCAACTCTCA GCCCATGGTT 
ACTAACTGGA TCCAGGAAAA AGTGTGGCTC TCCCAGGAGG TGGACAAACT GAGGGTGATG 
TTCCTGGAAA TGAAAAATGA GAAGGCAAAA CTCACAGTCA AGTTCCAGAG CCATAGAAAT 
ATCCTGGAGG AGAATCTTCG GCGTTCTGAC AAGGAGTTAA AGAAACTAGA CGACATCGTT 
CAGCACATTT ATGAGACTCT GTTGTCCATC CCAGAGGTCG TGAGGGGTTG CAAGGAGCTT 
CAAGGATTGC TGGAATTTCT GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG5 ( XM_011359969.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358271.1 Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPt46672
Clone ID Related Accession (Same CDS sequence)	XM_011359969.1 , XM_011359969.2
Accession Version	XM_011359969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	sperm-associated antigen 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888831.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011359969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGATGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC TACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGGACTTT CTCTTTCAGA GATCCCAGAT 
ATGGAGAAGA GCCTGGCAGA AATGAGTACT ATCACTCTGG AGCTTCAGAG CCTGTGTTCC 
TTGTTGCAAG GGTCTAAAGA AGAAGCCGTC AGGAGTATGC AACAAAAGAT TTGTGATCTG 
CAGGCTAGGC TGCAGGCCCA GGAAGAACAG CATCAGGAAG CCCTGAAGGC AAAGGAAGCA 
GACATAGAGA AGCTGAACCA GGCCTTGTGC TTGCGCTACA AGAATGAAAA GGAGCTCCAG 
GAAGTGATAC AGCAACAGAA TGAGAAGATT CTAGAACAGA TAGACAAGAG TGGCGAGCTC 
ATAAGTCTTA GAGAGGAGGT GACCCAGCTT ACCCGCTCAC TTCGGCGTGC GGAGACAGAG 
ACTAAGGTGC TCCAAGAGAC CCTGGCAGGT CAGCTGGACC CCAACTCTCA GCCCATGGTT 
ACTAACTGGA TCCAGGAAAA AGTGTGGCTC TCCCAGGAGG TGGACAAACT GAGGGTGATG 
TTCCTGGAAA TGAAAAATGA GAAGGCAAAA CTCACAGTCA AGTTCCAGAG CCATAGAAAT 
ATCCTGGAGG AGAATCTTCG GCGTTCTGAC AAGGAGTTAA AGAAACTAGA CGACATCGTT 
CAGCACATTT ATGAGACTCT GTTGTCCATC CCAGAGGTCG TGAGGGGTTG CAAGGAGCTT 
CAAGGATTGC TGGAATTTCT GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358271.1
CDS	23..3709
Translation	MKTAGEQVVSFWVCQVVVVRSMLKMWRVKKVNLSLSLSPQPGKPAMRTPLRELNLHPDALTSSGKGPPMGYSLTPSLCKLELQECTNNSSLLGFENPKMTDSPSEQCSHPSKWLEACQHESDEQPLNLIPQTNSTPKTSEEAVDPLDNNVIKTMILVPSSPGGQQQDITLKAHLDTMAEINSFSLDKPLRPGDLLRKGETPCMEDSFSRVVPAMPEKPTFRNPPSHLLEYPSNSCSEQQLHCSRENLRDSSSEAVPEDLVASETNAILPSSMLCLCPPTALAADFLVSHVDPGEETVEHRAIEEGEMNFPTLTEEAELGDQARVLNMEDIPSSCLIPNPGEMESQAAPCPAVEDAGRILNSDTGPWMSPLAWLEKGVNTSVMLENLRQSLSFPSVLRDAATGTTPFSTCSVGTWFTPPVPQEKSTNTSQTGLVGTKDNTSEREHLLWGHPPDLTALSRHDLEDNLMNSLVILEVLSHQLQNWKSQLTIPHPEVQDSSTQTDTTDTSPSGISKKSQHLLESQEVGQALLQARNVLQSWALVSKELISLLHLSLLHLEEDKTTVSQESRRAETLISCCFDVLKKLKARLQSLKAEREEAKHREEMALRGKDAAETVLEAFCAHASQRISQLKQDLACMGEFRGLLKETQTQLVGLHTEKEELAQQTVSLISVLQQDWISMQLDYITWTALLSRSRQLTEKLTAKRQQTLQERDTAIEEKQQVSRELERVSTQLEDCKGQIGQLELENSRLAADLQAQLQILASMQSQLEELQSQHAHCTQDLATKDELLCQLTQSNEEQATQWQKEEMALKHMQAKLQQQHAVLAKEVQDLKETLEFADQENQVAHLELGQVECQLKTTLEVLRERSLQCEDLRDTVENLKAQLASTIAENQKKDLEKTRQYSQELRGLTKQLQSLTLFLQTKLKEKAEPETLLKSTACAPAQEHPLSTDSTFLGSISTAVADKEPESPPMSLLGSDKSAFTRVASMASLQPTGLSLSEIPDMEKSLAEMSTITLELQSLCSLLQGSKEEAVRSMQQKICDLQARLQAQEEQHQEALKAKEADIEKLNQALCLRYKNEKELQEVIQQQNEKILEQIDKSGELISLREEVTQLTRSLRRAETETKVLQETLAGQLDPNSQPMVTNWIQEKVWLSQEVDKLRVMFLEMKNEKAKLTVKFQSHRNILEENLRRSDKELKKLDDIVQHIYETLLSIPEVVRGCKELQGLLEFLS

Target ORF information:

RefSeq Version	XM_011359969.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011359969.2

ORF Insert Sequence:

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGATGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC TACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGGACTTT CTCTTTCAGA GATCCCAGAT 
ATGGAGAAGA GCCTGGCAGA AATGAGTACT ATCACTCTGG AGCTTCAGAG CCTGTGTTCC 
TTGTTGCAAG GGTCTAAAGA AGAAGCCGTC AGGAGTATGC AACAAAAGAT TTGTGATCTG 
CAGGCTAGGC TGCAGGCCCA GGAAGAACAG CATCAGGAAG CCCTGAAGGC AAAGGAAGCA 
GACATAGAGA AGCTGAACCA GGCCTTGTGC TTGCGCTACA AGAATGAAAA GGAGCTCCAG 
GAAGTGATAC AGCAACAGAA TGAGAAGATT CTAGAACAGA TAGACAAGAG TGGCGAGCTC 
ATAAGTCTTA GAGAGGAGGT GACCCAGCTT ACCCGCTCAC TTCGGCGTGC GGAGACAGAG 
ACTAAGGTGC TCCAAGAGAC CCTGGCAGGT CAGCTGGACC CCAACTCTCA GCCCATGGTT 
ACTAACTGGA TCCAGGAAAA AGTGTGGCTC TCCCAGGAGG TGGACAAACT GAGGGTGATG 
TTCCTGGAAA TGAAAAATGA GAAGGCAAAA CTCACAGTCA AGTTCCAGAG CCATAGAAAT 
ATCCTGGAGG AGAATCTTCG GCGTTCTGAC AAGGAGTTAA AGAAACTAGA CGACATCGTT 
CAGCACATTT ATGAGACTCT GTTGTCCATC CCAGAGGTCG TGAGGGGTTG CAAGGAGCTT 
CAAGGATTGC TGGAATTTCT GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG5 ( XM_011359970.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358272.1 Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X2, mRNA.

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CloneID	OPt46673
Clone ID Related Accession (Same CDS sequence)	XM_011359970.2 , XM_011359970.1
Accession Version	XM_011359970.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	sperm-associated antigen 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888831.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011359970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGATGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC TACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGAGATCC CAGATATGGA GAAGAGCCTG 
GCAGAAATGA GTACTATCAC TCTGGAGCTT CAGAGCCTGT GTTCCTTGTT GCAAGGGTCT 
AAAGAAGAAG CCGTCAGGAG TATGCAACAA AAGATTTGTG ATCTGCAGGC TAGGCTGCAG 
GCCCAGGAAG AACAGCATCA GGAAGCCCTG AAGGCAAAGG AAGCAGACAT AGAGAAGCTG 
AACCAGGCCT TGTGCTTGCG CTACAAGAAT GAAAAGGAGC TCCAGGAAGT GATACAGCAA 
CAGAATGAGA AGATTCTAGA ACAGATAGAC AAGAGTGGCG AGCTCATAAG TCTTAGAGAG 
GAGGTGACCC AGCTTACCCG CTCACTTCGG CGTGCGGAGA CAGAGACTAA GGTGCTCCAA 
GAGACCCTGG CAGGTCAGCT GGACCCCAAC TCTCAGCCCA TGGTTACTAA CTGGATCCAG 
GAAAAAGTGT GGCTCTCCCA GGAGGTGGAC AAACTGAGGG TGATGTTCCT GGAAATGAAA 
AATGAGAAGG CAAAACTCAC AGTCAAGTTC CAGAGCCATA GAAATATCCT GGAGGAGAAT 
CTTCGGCGTT CTGACAAGGA GTTAAAGAAA CTAGACGACA TCGTTCAGCA CATTTATGAG 
ACTCTGTTGT CCATCCCAGA GGTCGTGAGG GGTTGCAAGG AGCTTCAAGG ATTGCTGGAA 
TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358272.1
CDS	23..3694
Translation	MKTAGEQVVSFWVCQVVVVRSMLKMWRVKKVNLSLSLSPQPGKPAMRTPLRELNLHPDALTSSGKGPPMGYSLTPSLCKLELQECTNNSSLLGFENPKMTDSPSEQCSHPSKWLEACQHESDEQPLNLIPQTNSTPKTSEEAVDPLDNNVIKTMILVPSSPGGQQQDITLKAHLDTMAEINSFSLDKPLRPGDLLRKGETPCMEDSFSRVVPAMPEKPTFRNPPSHLLEYPSNSCSEQQLHCSRENLRDSSSEAVPEDLVASETNAILPSSMLCLCPPTALAADFLVSHVDPGEETVEHRAIEEGEMNFPTLTEEAELGDQARVLNMEDIPSSCLIPNPGEMESQAAPCPAVEDAGRILNSDTGPWMSPLAWLEKGVNTSVMLENLRQSLSFPSVLRDAATGTTPFSTCSVGTWFTPPVPQEKSTNTSQTGLVGTKDNTSEREHLLWGHPPDLTALSRHDLEDNLMNSLVILEVLSHQLQNWKSQLTIPHPEVQDSSTQTDTTDTSPSGISKKSQHLLESQEVGQALLQARNVLQSWALVSKELISLLHLSLLHLEEDKTTVSQESRRAETLISCCFDVLKKLKARLQSLKAEREEAKHREEMALRGKDAAETVLEAFCAHASQRISQLKQDLACMGEFRGLLKETQTQLVGLHTEKEELAQQTVSLISVLQQDWISMQLDYITWTALLSRSRQLTEKLTAKRQQTLQERDTAIEEKQQVSRELERVSTQLEDCKGQIGQLELENSRLAADLQAQLQILASMQSQLEELQSQHAHCTQDLATKDELLCQLTQSNEEQATQWQKEEMALKHMQAKLQQQHAVLAKEVQDLKETLEFADQENQVAHLELGQVECQLKTTLEVLRERSLQCEDLRDTVENLKAQLASTIAENQKKDLEKTRQYSQELRGLTKQLQSLTLFLQTKLKEKAEPETLLKSTACAPAQEHPLSTDSTFLGSISTAVADKEPESPPMSLLGSDKSAFTRVASMASLQPTEIPDMEKSLAEMSTITLELQSLCSLLQGSKEEAVRSMQQKICDLQARLQAQEEQHQEALKAKEADIEKLNQALCLRYKNEKELQEVIQQQNEKILEQIDKSGELISLREEVTQLTRSLRRAETETKVLQETLAGQLDPNSQPMVTNWIQEKVWLSQEVDKLRVMFLEMKNEKAKLTVKFQSHRNILEENLRRSDKELKKLDDIVQHIYETLLSIPEVVRGCKELQGLLEFLS

Target ORF information:

RefSeq Version	XM_011359970.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011359970.2

ORF Insert Sequence:

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGATGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC TACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGAGATCC CAGATATGGA GAAGAGCCTG 
GCAGAAATGA GTACTATCAC TCTGGAGCTT CAGAGCCTGT GTTCCTTGTT GCAAGGGTCT 
AAAGAAGAAG CCGTCAGGAG TATGCAACAA AAGATTTGTG ATCTGCAGGC TAGGCTGCAG 
GCCCAGGAAG AACAGCATCA GGAAGCCCTG AAGGCAAAGG AAGCAGACAT AGAGAAGCTG 
AACCAGGCCT TGTGCTTGCG CTACAAGAAT GAAAAGGAGC TCCAGGAAGT GATACAGCAA 
CAGAATGAGA AGATTCTAGA ACAGATAGAC AAGAGTGGCG AGCTCATAAG TCTTAGAGAG 
GAGGTGACCC AGCTTACCCG CTCACTTCGG CGTGCGGAGA CAGAGACTAA GGTGCTCCAA 
GAGACCCTGG CAGGTCAGCT GGACCCCAAC TCTCAGCCCA TGGTTACTAA CTGGATCCAG 
GAAAAAGTGT GGCTCTCCCA GGAGGTGGAC AAACTGAGGG TGATGTTCCT GGAAATGAAA 
AATGAGAAGG CAAAACTCAC AGTCAAGTTC CAGAGCCATA GAAATATCCT GGAGGAGAAT 
CTTCGGCGTT CTGACAAGGA GTTAAAGAAA CTAGACGACA TCGTTCAGCA CATTTATGAG 
ACTCTGTTGT CCATCCCAGA GGTCGTGAGG GGTTGCAAGG AGCTTCAAGG ATTGCTGGAA 
TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG5 ( XM_011359970.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358272.1 Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPt46673
Clone ID Related Accession (Same CDS sequence)	XM_011359970.2 , XM_011359970.1
Accession Version	XM_011359970.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	sperm-associated antigen 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888831.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGATGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC TACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGAGATCC CAGATATGGA GAAGAGCCTG 
GCAGAAATGA GTACTATCAC TCTGGAGCTT CAGAGCCTGT GTTCCTTGTT GCAAGGGTCT 
AAAGAAGAAG CCGTCAGGAG TATGCAACAA AAGATTTGTG ATCTGCAGGC TAGGCTGCAG 
GCCCAGGAAG AACAGCATCA GGAAGCCCTG AAGGCAAAGG AAGCAGACAT AGAGAAGCTG 
AACCAGGCCT TGTGCTTGCG CTACAAGAAT GAAAAGGAGC TCCAGGAAGT GATACAGCAA 
CAGAATGAGA AGATTCTAGA ACAGATAGAC AAGAGTGGCG AGCTCATAAG TCTTAGAGAG 
GAGGTGACCC AGCTTACCCG CTCACTTCGG CGTGCGGAGA CAGAGACTAA GGTGCTCCAA 
GAGACCCTGG CAGGTCAGCT GGACCCCAAC TCTCAGCCCA TGGTTACTAA CTGGATCCAG 
GAAAAAGTGT GGCTCTCCCA GGAGGTGGAC AAACTGAGGG TGATGTTCCT GGAAATGAAA 
AATGAGAAGG CAAAACTCAC AGTCAAGTTC CAGAGCCATA GAAATATCCT GGAGGAGAAT 
CTTCGGCGTT CTGACAAGGA GTTAAAGAAA CTAGACGACA TCGTTCAGCA CATTTATGAG 
ACTCTGTTGT CCATCCCAGA GGTCGTGAGG GGTTGCAAGG AGCTTCAAGG ATTGCTGGAA 
TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358272.1
CDS	22..3693
Translation	MKTAGEQVVSFWVCQVVVVRSMLKMWRVKKVNLSLSLSPQPGKPAMRTPLRELNLHPDALTSSGKGPPMGYSLTPSLCKLELQECTNNSSLLGFENPKMTDSPSEQCSHPSKWLEACQHESDEQPLNLIPQTNSTPKTSEEAVDPLDNNVIKTMILVPSSPGGQQQDITLKAHLDTMAEINSFSLDKPLRPGDLLRKGETPCMEDSFSRVVPAMPEKPTFRNPPSHLLEYPSNSCSEQQLHCSRENLRDSSSEAVPEDLVASETNAILPSSMLCLCPPTALAADFLVSHVDPGEETVEHRAIEEGEMNFPTLTEEAELGDQARVLNMEDIPSSCLIPNPGEMESQAAPCPAVEDAGRILNSDTGPWMSPLAWLEKGVNTSVMLENLRQSLSFPSVLRDAATGTTPFSTCSVGTWFTPPVPQEKSTNTSQTGLVGTKDNTSEREHLLWGHPPDLTALSRHDLEDNLMNSLVILEVLSHQLQNWKSQLTIPHPEVQDSSTQTDTTDTSPSGISKKSQHLLESQEVGQALLQARNVLQSWALVSKELISLLHLSLLHLEEDKTTVSQESRRAETLISCCFDVLKKLKARLQSLKAEREEAKHREEMALRGKDAAETVLEAFCAHASQRISQLKQDLACMGEFRGLLKETQTQLVGLHTEKEELAQQTVSLISVLQQDWISMQLDYITWTALLSRSRQLTEKLTAKRQQTLQERDTAIEEKQQVSRELERVSTQLEDCKGQIGQLELENSRLAADLQAQLQILASMQSQLEELQSQHAHCTQDLATKDELLCQLTQSNEEQATQWQKEEMALKHMQAKLQQQHAVLAKEVQDLKETLEFADQENQVAHLELGQVECQLKTTLEVLRERSLQCEDLRDTVENLKAQLASTIAENQKKDLEKTRQYSQELRGLTKQLQSLTLFLQTKLKEKAEPETLLKSTACAPAQEHPLSTDSTFLGSISTAVADKEPESPPMSLLGSDKSAFTRVASMASLQPTEIPDMEKSLAEMSTITLELQSLCSLLQGSKEEAVRSMQQKICDLQARLQAQEEQHQEALKAKEADIEKLNQALCLRYKNEKELQEVIQQQNEKILEQIDKSGELISLREEVTQLTRSLRRAETETKVLQETLAGQLDPNSQPMVTNWIQEKVWLSQEVDKLRVMFLEMKNEKAKLTVKFQSHRNILEENLRRSDKELKKLDDIVQHIYETLLSIPEVVRGCKELQGLLEFLS

Target ORF information:

RefSeq Version	XM_011359970.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011359970.1

ORF Insert Sequence:

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGATGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC TACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGAGATCC CAGATATGGA GAAGAGCCTG 
GCAGAAATGA GTACTATCAC TCTGGAGCTT CAGAGCCTGT GTTCCTTGTT GCAAGGGTCT 
AAAGAAGAAG CCGTCAGGAG TATGCAACAA AAGATTTGTG ATCTGCAGGC TAGGCTGCAG 
GCCCAGGAAG AACAGCATCA GGAAGCCCTG AAGGCAAAGG AAGCAGACAT AGAGAAGCTG 
AACCAGGCCT TGTGCTTGCG CTACAAGAAT GAAAAGGAGC TCCAGGAAGT GATACAGCAA 
CAGAATGAGA AGATTCTAGA ACAGATAGAC AAGAGTGGCG AGCTCATAAG TCTTAGAGAG 
GAGGTGACCC AGCTTACCCG CTCACTTCGG CGTGCGGAGA CAGAGACTAA GGTGCTCCAA 
GAGACCCTGG CAGGTCAGCT GGACCCCAAC TCTCAGCCCA TGGTTACTAA CTGGATCCAG 
GAAAAAGTGT GGCTCTCCCA GGAGGTGGAC AAACTGAGGG TGATGTTCCT GGAAATGAAA 
AATGAGAAGG CAAAACTCAC AGTCAAGTTC CAGAGCCATA GAAATATCCT GGAGGAGAAT 
CTTCGGCGTT CTGACAAGGA GTTAAAGAAA CTAGACGACA TCGTTCAGCA CATTTATGAG 
ACTCTGTTGT CCATCCCAGA GGTCGTGAGG GGTTGCAAGG AGCTTCAAGG ATTGCTGGAA 
TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG5 ( XM_011359971.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358273.1 Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPt46674
Clone ID Related Accession (Same CDS sequence)	XM_011359971.2 , XM_011359971.1
Accession Version	XM_011359971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	sperm-associated antigen 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888831.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011359971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGGAGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC CACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGAGATCC CAGATATGGA GAAGAGCCTG 
GCAGAAATGA GTACTATCAC TCTGGAGCTT CAGAGCCTGT GTTCCTTGTT GCAAGGGTCT 
AAAGAAGAAG CCGTCAGGAG TATGCAACAA AAGATTTGTG ATCTGCAGGC TAGGCTGCAG 
GCCCAGGAAG AACAGCATCA GGAAGCCCTG AAGGCAAAGG AAGCAGACAT AGAGAAGCTG 
AACCAGGCCT TGTGCTTGCG CTACAAGAAT GAAAAGGAGC TCCAGGAAGT GATACAGCAA 
CAGAATGAGA AGATTCTAGA ACAGATAGAC AAGAGTGGCG AGCTCATAAG TCTTAGAGAG 
GAGGTGACCC AGCTTACCCG CTCACTTCGG CGTGCGGAGA CAGAGACTAA GGTGCTCCAA 
GAGACCCTGG CAGGTCAGCT GGACCCCAAC TCTCAGCCCA TGGTTACTAA CTGGATCCAG 
GAAAAAGTGT GGCTCTCCCA GGAGGTGGAC AAACTGAGGG TGATGTTCCT GGAAATGAAA 
AATGAGAAGG CAAAACTCAC AGTCAAGTTC CAGAGCCATA GAAATATCCT GGAGGAGAAT 
CTTCGGCGTT CTGACAAGGA GTTAAAGAAA CTAGACGACA TCGTTCAGCA CATTTATGAG 
ACTCTGTTGT CCATCCCAGA GGTCGTGAGG GGTTGCAAGG AGCTTCAAGG ATTGCTGGAA 
TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358273.1
CDS	38..3709
Translation	MKTAGEQVVSFWVCQVVVVRSMLKMWRVKKVNLSLSLSPQPGKPAMRTPLRELNLHPGALTSSGKGPPMGHSLTPSLCKLELQECTNNSSLLGFENPKMTDSPSEQCSHPSKWLEACQHESDEQPLNLIPQTNSTPKTSEEAVDPLDNNVIKTMILVPSSPGGQQQDITLKAHLDTMAEINSFSLDKPLRPGDLLRKGETPCMEDSFSRVVPAMPEKPTFRNPPSHLLEYPSNSCSEQQLHCSRENLRDSSSEAVPEDLVASETNAILPSSMLCLCPPTALAADFLVSHVDPGEETVEHRAIEEGEMNFPTLTEEAELGDQARVLNMEDIPSSCLIPNPGEMESQAAPCPAVEDAGRILNSDTGPWMSPLAWLEKGVNTSVMLENLRQSLSFPSVLRDAATGTTPFSTCSVGTWFTPPVPQEKSTNTSQTGLVGTKDNTSEREHLLWGHPPDLTALSRHDLEDNLMNSLVILEVLSHQLQNWKSQLTIPHPEVQDSSTQTDTTDTSPSGISKKSQHLLESQEVGQALLQARNVLQSWALVSKELISLLHLSLLHLEEDKTTVSQESRRAETLISCCFDVLKKLKARLQSLKAEREEAKHREEMALRGKDAAETVLEAFCAHASQRISQLKQDLACMGEFRGLLKETQTQLVGLHTEKEELAQQTVSLISVLQQDWISMQLDYITWTALLSRSRQLTEKLTAKRQQTLQERDTAIEEKQQVSRELERVSTQLEDCKGQIGQLELENSRLAADLQAQLQILASMQSQLEELQSQHAHCTQDLATKDELLCQLTQSNEEQATQWQKEEMALKHMQAKLQQQHAVLAKEVQDLKETLEFADQENQVAHLELGQVECQLKTTLEVLRERSLQCEDLRDTVENLKAQLASTIAENQKKDLEKTRQYSQELRGLTKQLQSLTLFLQTKLKEKAEPETLLKSTACAPAQEHPLSTDSTFLGSISTAVADKEPESPPMSLLGSDKSAFTRVASMASLQPTEIPDMEKSLAEMSTITLELQSLCSLLQGSKEEAVRSMQQKICDLQARLQAQEEQHQEALKAKEADIEKLNQALCLRYKNEKELQEVIQQQNEKILEQIDKSGELISLREEVTQLTRSLRRAETETKVLQETLAGQLDPNSQPMVTNWIQEKVWLSQEVDKLRVMFLEMKNEKAKLTVKFQSHRNILEENLRRSDKELKKLDDIVQHIYETLLSIPEVVRGCKELQGLLEFLS

Target ORF information:

RefSeq Version	XM_011359971.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011359971.2

ORF Insert Sequence:

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGGAGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC CACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGAGATCC CAGATATGGA GAAGAGCCTG 
GCAGAAATGA GTACTATCAC TCTGGAGCTT CAGAGCCTGT GTTCCTTGTT GCAAGGGTCT 
AAAGAAGAAG CCGTCAGGAG TATGCAACAA AAGATTTGTG ATCTGCAGGC TAGGCTGCAG 
GCCCAGGAAG AACAGCATCA GGAAGCCCTG AAGGCAAAGG AAGCAGACAT AGAGAAGCTG 
AACCAGGCCT TGTGCTTGCG CTACAAGAAT GAAAAGGAGC TCCAGGAAGT GATACAGCAA 
CAGAATGAGA AGATTCTAGA ACAGATAGAC AAGAGTGGCG AGCTCATAAG TCTTAGAGAG 
GAGGTGACCC AGCTTACCCG CTCACTTCGG CGTGCGGAGA CAGAGACTAA GGTGCTCCAA 
GAGACCCTGG CAGGTCAGCT GGACCCCAAC TCTCAGCCCA TGGTTACTAA CTGGATCCAG 
GAAAAAGTGT GGCTCTCCCA GGAGGTGGAC AAACTGAGGG TGATGTTCCT GGAAATGAAA 
AATGAGAAGG CAAAACTCAC AGTCAAGTTC CAGAGCCATA GAAATATCCT GGAGGAGAAT 
CTTCGGCGTT CTGACAAGGA GTTAAAGAAA CTAGACGACA TCGTTCAGCA CATTTATGAG 
ACTCTGTTGT CCATCCCAGA GGTCGTGAGG GGTTGCAAGG AGCTTCAAGG ATTGCTGGAA 
TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG5 ( XM_011359971.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011358273.1 Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X3, mRNA.

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CloneID	OPt46674
Clone ID Related Accession (Same CDS sequence)	XM_011359971.2 , XM_011359971.1
Accession Version	XM_011359971.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	sperm-associated antigen 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888831.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGGAGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC CACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGAGATCC CAGATATGGA GAAGAGCCTG 
GCAGAAATGA GTACTATCAC TCTGGAGCTT CAGAGCCTGT GTTCCTTGTT GCAAGGGTCT 
AAAGAAGAAG CCGTCAGGAG TATGCAACAA AAGATTTGTG ATCTGCAGGC TAGGCTGCAG 
GCCCAGGAAG AACAGCATCA GGAAGCCCTG AAGGCAAAGG AAGCAGACAT AGAGAAGCTG 
AACCAGGCCT TGTGCTTGCG CTACAAGAAT GAAAAGGAGC TCCAGGAAGT GATACAGCAA 
CAGAATGAGA AGATTCTAGA ACAGATAGAC AAGAGTGGCG AGCTCATAAG TCTTAGAGAG 
GAGGTGACCC AGCTTACCCG CTCACTTCGG CGTGCGGAGA CAGAGACTAA GGTGCTCCAA 
GAGACCCTGG CAGGTCAGCT GGACCCCAAC TCTCAGCCCA TGGTTACTAA CTGGATCCAG 
GAAAAAGTGT GGCTCTCCCA GGAGGTGGAC AAACTGAGGG TGATGTTCCT GGAAATGAAA 
AATGAGAAGG CAAAACTCAC AGTCAAGTTC CAGAGCCATA GAAATATCCT GGAGGAGAAT 
CTTCGGCGTT CTGACAAGGA GTTAAAGAAA CTAGACGACA TCGTTCAGCA CATTTATGAG 
ACTCTGTTGT CCATCCCAGA GGTCGTGAGG GGTTGCAAGG AGCTTCAAGG ATTGCTGGAA 
TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011358273.1
CDS	12..3683
Translation	MKTAGEQVVSFWVCQVVVVRSMLKMWRVKKVNLSLSLSPQPGKPAMRTPLRELNLHPGALTSSGKGPPMGHSLTPSLCKLELQECTNNSSLLGFENPKMTDSPSEQCSHPSKWLEACQHESDEQPLNLIPQTNSTPKTSEEAVDPLDNNVIKTMILVPSSPGGQQQDITLKAHLDTMAEINSFSLDKPLRPGDLLRKGETPCMEDSFSRVVPAMPEKPTFRNPPSHLLEYPSNSCSEQQLHCSRENLRDSSSEAVPEDLVASETNAILPSSMLCLCPPTALAADFLVSHVDPGEETVEHRAIEEGEMNFPTLTEEAELGDQARVLNMEDIPSSCLIPNPGEMESQAAPCPAVEDAGRILNSDTGPWMSPLAWLEKGVNTSVMLENLRQSLSFPSVLRDAATGTTPFSTCSVGTWFTPPVPQEKSTNTSQTGLVGTKDNTSEREHLLWGHPPDLTALSRHDLEDNLMNSLVILEVLSHQLQNWKSQLTIPHPEVQDSSTQTDTTDTSPSGISKKSQHLLESQEVGQALLQARNVLQSWALVSKELISLLHLSLLHLEEDKTTVSQESRRAETLISCCFDVLKKLKARLQSLKAEREEAKHREEMALRGKDAAETVLEAFCAHASQRISQLKQDLACMGEFRGLLKETQTQLVGLHTEKEELAQQTVSLISVLQQDWISMQLDYITWTALLSRSRQLTEKLTAKRQQTLQERDTAIEEKQQVSRELERVSTQLEDCKGQIGQLELENSRLAADLQAQLQILASMQSQLEELQSQHAHCTQDLATKDELLCQLTQSNEEQATQWQKEEMALKHMQAKLQQQHAVLAKEVQDLKETLEFADQENQVAHLELGQVECQLKTTLEVLRERSLQCEDLRDTVENLKAQLASTIAENQKKDLEKTRQYSQELRGLTKQLQSLTLFLQTKLKEKAEPETLLKSTACAPAQEHPLSTDSTFLGSISTAVADKEPESPPMSLLGSDKSAFTRVASMASLQPTEIPDMEKSLAEMSTITLELQSLCSLLQGSKEEAVRSMQQKICDLQARLQAQEEQHQEALKAKEADIEKLNQALCLRYKNEKELQEVIQQQNEKILEQIDKSGELISLREEVTQLTRSLRRAETETKVLQETLAGQLDPNSQPMVTNWIQEKVWLSQEVDKLRVMFLEMKNEKAKLTVKFQSHRNILEENLRRSDKELKKLDDIVQHIYETLLSIPEVVRGCKELQGLLEFLS

Target ORF information:

RefSeq Version	XM_011359971.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus sperm associated antigen 5 (SPAG5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011359971.1

ORF Insert Sequence:

ATGAAGACGG CGGGTGAACA AGTAGTCAGC TTTTGGGTCT GCCAGGTGGT CGTCGTACGT 
TCTATGTTGA AGATGTGGCG GGTGAAAAAA GTGAACCTTA GCCTGTCGCT TTCGCCCCAG 
CCGGGAAAAC CAGCTATGAG AACTCCTCTC CGAGAACTTA ACTTGCACCC CGGAGCCCTC 
ACCAGCTCTG GAAAAGGGCC CCCGATGGGC CACTCGCTGA CCCCATCACT GTGCAAACTG 
GAGCTGCAGG AATGTACCAA CAACTCATCT CTACTGGGTT TTGAAAATCC TAAGATGACA 
GACTCTCCAT CAGAACAGTG CAGCCATCCG TCAAAGTGGT TAGAAGCTTG TCAACATGAA 
TCAGATGAGC AGCCCCTAAA TCTGATTCCT CAAACCAACT CTACTCCCAA AACGTCTGAG 
GAAGCAGTAG ACCCACTGGA CAACAATGTG ATTAAAACTA TGATCCTTGT ACCCTCCTCC 
CCAGGAGGAC AGCAGCAAGA CATTACACTT AAAGCCCATT TAGATACTAT GGCAGAAATA 
AACAGTTTCT CTCTAGATAA GCCTTTGAGG CCAGGAGATC TGCTGAGAAA GGGGGAGACA 
CCGTGCATGG AAGACAGCTT TTCAAGAGTT GTTCCCGCTA TGCCTGAGAA ACCTACATTT 
CGGAACCCTC CATCCCATCT CTTAGAGTAT CCATCAAATT CCTGTTCTGA GCAACAACTA 
CACTGTTCCA GGGAAAACTT GAGGGACAGT AGTTCTGAGG CTGTGCCTGA GGACTTAGTT 
GCTTCTGAAA CTAATGCCAT TTTGCCTTCC TCCATGCTCT GTCTTTGCCC TCCAACTGCC 
TTAGCAGCAG ATTTCCTTGT CAGTCATGTG GACCCAGGGG AGGAAACTGT GGAGCACAGA 
GCTATAGAGG AAGGAGAAAT GAACTTTCCC ACACTCACTG AGGAGGCTGA ATTGGGAGAT 
CAAGCACGTG TCTTAAATAT GGAAGATATT CCATCCTCAT GCCTGATCCC AAATCCAGGA 
GAAATGGAAT CCCAGGCAGC TCCATGCCCA GCAGTAGAAG ATGCTGGTAG GATTCTGAAC 
TCTGATACAG GGCCTTGGAT GTCCCCACTG GCCTGGCTGG AAAAAGGTGT AAATACCTCA 
GTCATGCTAG AAAATCTCCG CCAGAGCTTA TCCTTTCCCT CTGTGCTTCG GGATGCTGCA 
ACTGGCACTA CCCCTTTCTC TACTTGCTCA GTAGGGACTT GGTTTACCCC TCCAGTGCCA 
CAGGAAAAGA GTACAAACAC ATCACAGACA GGCCTAGTAG GCACCAAGGA CAATACTTCT 
GAGAGGGAGC ATCTCCTATG GGGCCATCCT CCAGATCTGA CTGCCTTATC TCGACACGAC 
CTGGAAGATA ACCTGATGAA CTCTCTTGTC ATTCTGGAGG TTCTCTCCCA CCAGCTGCAG 
AACTGGAAGA GCCAGCTGAC TATTCCTCAC CCAGAAGTTC AGGACAGTAG CACACAGACT 
GACACAACTG ACACCTCTCC CAGTGGGATA AGTAAGAAAT CTCAGCATCT TCTGGAGAGC 
CAAGAGGTTG GACAGGCCCT GCTGCAGGCC AGAAATGTCC TGCAATCATG GGCGCTGGTC 
TCTAAAGAGC TGATATCCTT GCTTCACTTA TCTCTGTTGC ACCTAGAGGA AGATAAAACT 
ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA ACCTTGATCT CCTGCTGTTT TGATGTGTTG 
AAGAAATTGA AGGCAAGGCT CCAGAGCCTT AAAGCAGAAA GGGAAGAGGC AAAACACAGA 
GAGGAAATGG CCCTCAGAGG CAAGGATGCG GCAGAGACAG TGCTAGAGGC TTTCTGTGCA 
CATGCCAGCC AGCGCATCAG CCAGCTGAAA CAGGACCTGG CATGCATGGG GGAATTCAGA 
GGCCTCTTGA AGGAGACCCA GACGCAACTG GTAGGCCTTC ATACTGAGAA AGAAGAATTG 
GCTCAGCAGA CAGTGAGTCT TATTTCAGTC TTGCAACAGG ATTGGATTTC CATGCAACTG 
GATTATATAA CATGGACGGC TTTGCTGAGC CGGTCTCGAC AACTCACAGA GAAACTCACA 
GCCAAGAGAC AGCAGACCCT GCAGGAACGT GATACTGCAA TTGAGGAAAA ACAGCAGGTT 
TCCAGGGAGC TGGAACGAGT CTCTACCCAG TTAGAGGACT GCAAAGGCCA AATAGGACAA 
TTGGAGTTGG AAAACAGTCG CCTAGCAGCA GATCTCCAGG CTCAACTGCA GATTCTTGCC 
AGCATGCAGA GTCAGCTAGA AGAGCTACAG AGTCAGCATG CTCACTGTAC CCAGGACCTG 
GCCACGAAGG ATGAGTTGCT CTGCCAGCTT ACCCAGAGCA ATGAAGAGCA GGCTACTCAA 
TGGCAAAAGG AAGAGATGGC ACTAAAACAC ATGCAGGCAA AGCTGCAGCA ACAACATGCT 
GTCCTGGCCA AGGAGGTGCA GGACCTGAAG GAGACACTGG AGTTTGCAGA CCAAGAGAAT 
CAGGTTGCTC ACCTGGAGCT GGGCCAGGTT GAGTGTCAGT TGAAAACCAC ACTGGAAGTG 
CTCCGGGAGC GCAGCTTGCA GTGTGAGGAC CTCAGGGACA CCGTAGAGAA CCTGAAGGCT 
CAACTGGCTA GCACCATAGC AGAGAACCAG AAGAAAGACC TGGAGAAGAC ACGCCAGTAT 
TCCCAAGAGC TCAGAGGGCT GACTAAGCAA CTACAGAGCC TGACCCTCTT TCTACAGACA 
AAACTAAAGG AGAAGGCTGA ACCAGAGACC CTCCTGAAAA GCACAGCCTG TGCTCCTGCC 
CAGGAACACC CTCTATCCAC GGATAGCACC TTCTTGGGAA GCATCTCGAC AGCAGTGGCA 
GACAAAGAGC CAGAATCACC TCCCATGTCC TTGCTTGGAA GTGACAAGAG TGCTTTCACC 
CGAGTAGCAT CAATGGCTTC CCTTCAGCCT ACAGAGATCC CAGATATGGA GAAGAGCCTG 
GCAGAAATGA GTACTATCAC TCTGGAGCTT CAGAGCCTGT GTTCCTTGTT GCAAGGGTCT 
AAAGAAGAAG CCGTCAGGAG TATGCAACAA AAGATTTGTG ATCTGCAGGC TAGGCTGCAG 
GCCCAGGAAG AACAGCATCA GGAAGCCCTG AAGGCAAAGG AAGCAGACAT AGAGAAGCTG 
AACCAGGCCT TGTGCTTGCG CTACAAGAAT GAAAAGGAGC TCCAGGAAGT GATACAGCAA 
CAGAATGAGA AGATTCTAGA ACAGATAGAC AAGAGTGGCG AGCTCATAAG TCTTAGAGAG 
GAGGTGACCC AGCTTACCCG CTCACTTCGG CGTGCGGAGA CAGAGACTAA GGTGCTCCAA 
GAGACCCTGG CAGGTCAGCT GGACCCCAAC TCTCAGCCCA TGGTTACTAA CTGGATCCAG 
GAAAAAGTGT GGCTCTCCCA GGAGGTGGAC AAACTGAGGG TGATGTTCCT GGAAATGAAA 
AATGAGAAGG CAAAACTCAC AGTCAAGTTC CAGAGCCATA GAAATATCCT GGAGGAGAAT 
CTTCGGCGTT CTGACAAGGA GTTAAAGAAA CTAGACGACA TCGTTCAGCA CATTTATGAG 
ACTCTGTTGT CCATCCCAGA GGTCGTGAGG GGTTGCAAGG AGCTTCAAGG ATTGCTGGAA 
TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SPAG5 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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