EIF4G3 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF4G3
Entrez Gene ID	105290655
Full Name	eukaryotic translation initiation factor 4 gamma 3
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023529131.1	XP_023384899.1	eukaryotic translation initiation factor 4 gamma 3 isoform X2
XM_023529130.1	XP_023384898.1	eukaryotic translation initiation factor 4 gamma 3 isoform X1
XM_011358019.1	XP_011356321.1	eukaryotic translation initiation factor 4 gamma 3

Pathways from BioSystems
Gene Ontology

The following EIF4G3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF4G3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt68508	XM_023529131.1 Latest version!	Pteropus vampyrus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPt68507	XM_023529130.1 Latest version!	Pteropus vampyrus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPt45234	XM_011358019.1 Latest version!	Pteropus vampyrus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF4G3 ( XM_023529131.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023384899.1 Pteropus vampyrus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPt68508
Clone ID Related Accession (Same CDS sequence)	XM_023529131.1
Accession Version	XM_023529131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	eukaryotic translation initiation factor 4 gamma 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888817.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA 
AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT 
TTCCAGAGGC CTCAAATACA GCCTCCTAGA GCTACCATCC CGAACAGCAG TCCTTCTATT 
CGTCCTGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT 
AACCATCTTA CCATGCCGTA CCCAGTACCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC 
CGTCATAGTG GCCCTCCATA TGTCGGACCC CCACAACAGT ATCCAGTTCA ACCACCGGGG 
CCAGGTCCTT TTTATCCTGG ACCAGGACCT GGGGACTTCC CCAATGCTTA CGGAACGCCT 
TTTTACCCAA GTCAGCCAGT GTATCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG 
CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GAGATCCAAA CCAGGGGGGT 
AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCA GAAATCCCAC TCCACCCATA 
GGGAGGCCCA CATCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC 
AGCCCTGTGG TTTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT 
GCAGCTAGCG ACCAGAAGCA AGAGGAGAAG CCAAAACCAG ACCCAGTGTT AAAGTCTCCT 
TCCCCAGTGC TTAGGTTAGT CCTTAGTGGA GAGAAGAAAG AACAAGTGGG CCAGATGTCT 
GAGACTACTG CAGCAGAATC CATACCAGAG CTTCCTCTGC CTCCATCACC AACCGCTATT 
TCTCCAATTG CTCGAAGTAC AATTGCTTCC CCCACCGCTG CTGCTCGCAG TAGCCAGCCG 
ACATTCACCA CTGCTCTAGA CGACAGATGT GAACTCTCCT CTGCAAAAGA AGACACAGTT 
CCTATACCCA GCCCCACATC TTGCACAGAA ACATCAGATC CTTCACAAAC AGATGTTATC 
GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCTA ATGATATTCC ACTGATTTCT 
AGTACTAACC TAATTAATGA AATGAATGGA GTTAGTGAAA AATTACCAGC CACGGAGAGC 
ATTGTGGAAT TAGTAAAACA GGAGGTGTTG CCACTGACTC TTGAATTGGA GATTCTGGAA 
AATCCCCCAG AAGAAATGAA AGTGGAGTGC GTTTCAGCTC CCATCACCCC TTCCACAGTT 
CCTTCCTTTT CTCCATCTCC TCCAACTCCT CCAGCTTCTC CTCCTGCGCC AGTCATTGTC 
CCTGCTGCTG CCATGAACGT TAGTACTGCT AGTGCTCCCG CCACAGTCCA GAGAGTCTCA 
GAAGAGGATG AGAGTATAAG AACTTGCCTC AGTGAAGATG CAAAAGAGAT TCAGAACAAA 
ATAGAGGTAG AAGCAGATGG GCAAACAGAA GAGACTTTGG ATTCTCAGAA CTTAAGTTCA 
AGAAAGAGCC CTGTCCTAGC TCAGACAGCT ATAACTGCAC CAAAGATGTG GAAGAAACCA 
AAAGATCGGA CCCGAACCAC TGAAGAGGTA TTAGAAACAG AATTGGAGCC TAAAGCTGAA 
GAGGAGCTTT CAGGTGACAG AGTACTTGAA TCTGAACAGG ATAAAATGAG CCAAGGGTTT 
CATCCTGAAA GAGACTCCTC AGATCTAAAA AAAGTGAAAA CTGTGGAAGA AAATGGAGAA 
GAAGCTGAGC CTGTACATAA TGGTGCTGAG AGTGTTTCTG AGGGTGAAGC AATAGATGCT 
AATTCAGGCT ACACTGATAG TTCTGGTGAA GGGGTCACAT TCTCATTTAA ACCAGAATCC 
TGGAAGCCTG CTGATACTGA AGGCAAGAAG CAGTATGACA GGGAGTTTTT ATTGGACTTT 
CAGTTCATGC CAGCCTGTAT ACAGAAACCA GAGGGCCTGC CTCCCATCAC TGATGTGGTT 
CTTGACAAGA TCAACCAACC CAAATTGCCA ATGCGAACTC TGGATCCTCG GATTTTGCCT 
CGAGGACCAG ATTTTACTCC AGCCTTTGCA GATTTTGGAA GGCAAACTCC TGGTGGTAGA 
GGTGTACCTA TTTGCAAAGT ACAGAGCAGG CATGGATTGC CAATTCTGGA ACAGAGCAAA 
GCCCCAATTT GCACCTCACT GGCGATGTCG CACCCACCCC TGAAGAGCCT GCCTCTAGGG 
TTGTCAAACG TTGGACCACG GAGATCTCAA CCTGGCCAGA GGAGAGAGCC CAGGAAGATC 
ATCACAGTCT CTGTAAAAGA AGACGTGCAC CTGAAAAAGG CAGAGAATGC CTGGAAGCCC 
AGCCAAAAAC GAGACAGCCA AGCTGAGGAC CCCGAAAACA TTAAAACCCA GGAACTTTTT 
AGAAAAGTTC GAAGTATCTT AAATAAGTTG ACACCACAGA TGTTTAACCA GCTGATGAAG 
CAAGTGTCAG GTCTTACTGT TGACACAGAG GAGCGGCTGA AAGGAGTCAT TGACCTGGTC 
TTTGAGAAGG CTATTGATGA ACCCAGTTTC TCTGTGGCTT ACGCAAACAT GTGTCGATGT 
CTAGTAACGG TAAGAAGAGA GGAAGGAATA AAACCAGAAA TCTCCAAGAG CCATTTATTC 
TCCACAGCCT GTCAAATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023384899.1
CDS	724..3504
Translation	MNSQPQTRSPPSRTVPIHCTDNWKRRKVLEQTPVYRSLAGRGWIKYCIFAAGPRPAHHQFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLTMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIIVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQVGQMSETTAAESIPELPLPPSPTAISPIARSTIASPTAAARSSQPTFTTALDDRCELSSAKEDTVPIPSPTSCTETSDPSQTDVIDDDICKKSCSVAPNDIPLISSTNLINEMNGVSEKLPATESIVELVKQEVLPLTLELEILENPPEEMKVECVSAPITPSTVPSFSPSPPTPPASPPAPVIVPAAAMNVSTASAPATVQRVSEEDESIRTCLSEDAKEIQNKIEVEADGQTEETLDSQNLSSRKSPVLAQTAITAPKMWKKPKDRTRTTEEVLETELEPKAEEELSGDRVLESEQDKMSQGFHPERDSSDLKKVKTVEENGEEAEPVHNGAESVSEGEAIDANSGYTDSSGEGVTFSFKPESWKPADTEGKKQYDREFLLDFQFMPACIQKPEGLPPITDVVLDKINQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPICKVQSRHGLPILEQSKAPICTSLAMSHPPLKSLPLGLSNVGPRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQAEDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTVRREEGIKPEISKSHLFSTACQI

Target ORF information:

RefSeq Version	XM_023529131.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023529131.1

ORF Insert Sequence:

ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA 
AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT 
TTCCAGAGGC CTCAAATACA GCCTCCTAGA GCTACCATCC CGAACAGCAG TCCTTCTATT 
CGTCCTGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT 
AACCATCTTA CCATGCCGTA CCCAGTACCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC 
CGTCATAGTG GCCCTCCATA TGTCGGACCC CCACAACAGT ATCCAGTTCA ACCACCGGGG 
CCAGGTCCTT TTTATCCTGG ACCAGGACCT GGGGACTTCC CCAATGCTTA CGGAACGCCT 
TTTTACCCAA GTCAGCCAGT GTATCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG 
CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GAGATCCAAA CCAGGGGGGT 
AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCA GAAATCCCAC TCCACCCATA 
GGGAGGCCCA CATCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC 
AGCCCTGTGG TTTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT 
GCAGCTAGCG ACCAGAAGCA AGAGGAGAAG CCAAAACCAG ACCCAGTGTT AAAGTCTCCT 
TCCCCAGTGC TTAGGTTAGT CCTTAGTGGA GAGAAGAAAG AACAAGTGGG CCAGATGTCT 
GAGACTACTG CAGCAGAATC CATACCAGAG CTTCCTCTGC CTCCATCACC AACCGCTATT 
TCTCCAATTG CTCGAAGTAC AATTGCTTCC CCCACCGCTG CTGCTCGCAG TAGCCAGCCG 
ACATTCACCA CTGCTCTAGA CGACAGATGT GAACTCTCCT CTGCAAAAGA AGACACAGTT 
CCTATACCCA GCCCCACATC TTGCACAGAA ACATCAGATC CTTCACAAAC AGATGTTATC 
GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCTA ATGATATTCC ACTGATTTCT 
AGTACTAACC TAATTAATGA AATGAATGGA GTTAGTGAAA AATTACCAGC CACGGAGAGC 
ATTGTGGAAT TAGTAAAACA GGAGGTGTTG CCACTGACTC TTGAATTGGA GATTCTGGAA 
AATCCCCCAG AAGAAATGAA AGTGGAGTGC GTTTCAGCTC CCATCACCCC TTCCACAGTT 
CCTTCCTTTT CTCCATCTCC TCCAACTCCT CCAGCTTCTC CTCCTGCGCC AGTCATTGTC 
CCTGCTGCTG CCATGAACGT TAGTACTGCT AGTGCTCCCG CCACAGTCCA GAGAGTCTCA 
GAAGAGGATG AGAGTATAAG AACTTGCCTC AGTGAAGATG CAAAAGAGAT TCAGAACAAA 
ATAGAGGTAG AAGCAGATGG GCAAACAGAA GAGACTTTGG ATTCTCAGAA CTTAAGTTCA 
AGAAAGAGCC CTGTCCTAGC TCAGACAGCT ATAACTGCAC CAAAGATGTG GAAGAAACCA 
AAAGATCGGA CCCGAACCAC TGAAGAGGTA TTAGAAACAG AATTGGAGCC TAAAGCTGAA 
GAGGAGCTTT CAGGTGACAG AGTACTTGAA TCTGAACAGG ATAAAATGAG CCAAGGGTTT 
CATCCTGAAA GAGACTCCTC AGATCTAAAA AAAGTGAAAA CTGTGGAAGA AAATGGAGAA 
GAAGCTGAGC CTGTACATAA TGGTGCTGAG AGTGTTTCTG AGGGTGAAGC AATAGATGCT 
AATTCAGGCT ACACTGATAG TTCTGGTGAA GGGGTCACAT TCTCATTTAA ACCAGAATCC 
TGGAAGCCTG CTGATACTGA AGGCAAGAAG CAGTATGACA GGGAGTTTTT ATTGGACTTT 
CAGTTCATGC CAGCCTGTAT ACAGAAACCA GAGGGCCTGC CTCCCATCAC TGATGTGGTT 
CTTGACAAGA TCAACCAACC CAAATTGCCA ATGCGAACTC TGGATCCTCG GATTTTGCCT 
CGAGGACCAG ATTTTACTCC AGCCTTTGCA GATTTTGGAA GGCAAACTCC TGGTGGTAGA 
GGTGTACCTA TTTGCAAAGT ACAGAGCAGG CATGGATTGC CAATTCTGGA ACAGAGCAAA 
GCCCCAATTT GCACCTCACT GGCGATGTCG CACCCACCCC TGAAGAGCCT GCCTCTAGGG 
TTGTCAAACG TTGGACCACG GAGATCTCAA CCTGGCCAGA GGAGAGAGCC CAGGAAGATC 
ATCACAGTCT CTGTAAAAGA AGACGTGCAC CTGAAAAAGG CAGAGAATGC CTGGAAGCCC 
AGCCAAAAAC GAGACAGCCA AGCTGAGGAC CCCGAAAACA TTAAAACCCA GGAACTTTTT 
AGAAAAGTTC GAAGTATCTT AAATAAGTTG ACACCACAGA TGTTTAACCA GCTGATGAAG 
CAAGTGTCAG GTCTTACTGT TGACACAGAG GAGCGGCTGA AAGGAGTCAT TGACCTGGTC 
TTTGAGAAGG CTATTGATGA ACCCAGTTTC TCTGTGGCTT ACGCAAACAT GTGTCGATGT 
CTAGTAACGG TAAGAAGAGA GGAAGGAATA AAACCAGAAA TCTCCAAGAG CCATTTATTC 
TCCACAGCCT GTCAAATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G3 ( XM_023529130.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023384898.1 Pteropus vampyrus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPt68507
Clone ID Related Accession (Same CDS sequence)	XM_023529130.1
Accession Version	XM_023529130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	eukaryotic translation initiation factor 4 gamma 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888817.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA 
AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT 
TTCCAGAGGC CTCAAATACA GCCTCCTAGA GCTACCATCC CGAACAGCAG TCCTTCTATT 
CGTCCTGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT 
AACCATCTTA CCATGCCGTA CCCAGTACCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC 
CGTCATAGTG GCCCTCCATA TGTCGGACCC CCACAACAGT ATCCAGTTCA ACCACCGGGG 
CCAGGTCCTT TTTATCCTGG ACCAGGACCT GGGGACTTCC CCAATGCTTA CGGAACGCCT 
TTTTACCCAA GTCAGCCAGT GTATCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG 
CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GAGATCCAAA CCAGGGGGGT 
AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCA GAAATCCCAC TCCACCCATA 
GGGAGGCCCA CATCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC 
AGCCCTGTGG TTTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT 
GCAGCTAGCG ACCAGAAGCA AGAGGAGAAG CCAAAACCAG ACCCAGTGTT AAAGTCTCCT 
TCCCCAGTGC TTAGGTTAGT CCTTAGTGGA GAGAAGAAAG AACAAGTGGG CCAGATGTCT 
GAGACTACTG CAGCAGAATC CATACCAGAG CTTCCTCTGC CTCCATCACC AACCGCTATT 
TCTCCAATTG CTCGAAGTAC AATTGCTTCC CCCACCGCTG CTGCTCGCAG TAGCCAGCCG 
ACATTCACCA CTGCTCTAGA CGACAGATGT GAACTCTCCT CTGCAAAAGA AGACACAGTT 
CCTATACCCA GCCCCACATC TTGCACAGAA ACATCAGATC CTTCACAAAC AGATGTTATC 
GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCTA ATGATATTCC ACTGATTTCT 
AGTACTAACC TAATTAATGA AATGAATGGA GTTAGTGAAA AATTACCAGC CACGGAGAGC 
ATTGTGGAAT TAGTAAAACA GGAGGTGTTG CCACTGACTC TTGAATTGGA GATTCTGGAA 
AATCCCCCAG AAGAAATGAA AGTGGAGTGC GTTTCAGCTC CCATCACCCC TTCCACAGTT 
CCTTCCTTTT CTCCATCTCC TCCAACTCCT CCAGCTTCTC CTCCTGCGCC AGTCATTGTC 
CCTGCTGCTG CCATGAACGT TAGTACTGCT AGTGCTCCCG CCACAGTCCA GAGAGTCTCA 
GAAGAGGATG AGAGTATAAG AACTTGCCTC AGTGAAGATG CAAAAGAGAT TCAGAACAAA 
ATAGAGGTAG AAGCAGATGG GCAAACAGAA GAGACTTTGG ATTCTCAGAA CTTAAGTTCA 
AGAAAGAGCC CTGTCCTAGC TCAGACAGCT ATAACTGCAC CAAAGATGTG GAAGAAACCA 
AAAGATCGGA CCCGAACCAC TGAAGAGGTA TTAGAAACAG AATTGGAGCC TAAAGCTGAA 
GAGGAGCTTT CAGGTGACAG AGTACTTGAA TCTGAACAGG ATAAAATGAG CCAAGGGTTT 
CATCCTGAAA GAGACTCCTC AGATCTAAAA AAAGTGAAAA CTGTGGAAGA AAATGGAGAA 
GAAGCTGAGC CTGTACATAA TGGTGCTGAG AGTGTTTCTG AGGGTGAAGC AATAGATGCT 
AATTCAGGCT ACACTGATAG TTCTGGTGAA GGGGTCACAT TCTCATTTAA ACCAGAATCC 
TGGAAGCCTG CTGATACTGA AGGCAAGAAG CAGTATGACA GGGAGTTTTT ATTGGACTTT 
CAGTTCATGC CAGCCTGTAT ACAGAAACCA GAGGGCCTGC CTCCCATCAC TGATGTGGTT 
CTTGACAAGA TCAACCAACC CAAATTGCCA ATGCGAACTC TGGATCCTCG GATTTTGCCT 
CGAGGACCAG ATTTTACTCC AGCCTTTGCA GATTTTGGAA GGCAAACTCC TGGTGGTAGA 
GGTGTACCTA TTTGCAAAGT ACAGAGCAGG CATGGATTGC CAATTCTGGA ACAGAGCAAA 
GCCCCAATTT GCACCTCACT GGCGATGTCG CACCCACCCC TGAAGAGCCT GCCTCTAGGG 
TTGTCAAACG TTGGACCACG GAGATCTCAA CCTGGCCAGA GGAGAGAGCC CAGGAAGATC 
ATCACAGTCT CTGTAAAAGA AGACGTGCAC CTGAAAAAGG CAGAGAATGC CTGGAAGCCC 
AGCCAAAAAC GAGACAGCCA AGCTGAGGAC CCCGAAAACA TTAAAACCCA GGAACTTTTT 
AGAAAAGTTC GAAGTATCTT AAATAAGTTG ACACCACAGA TGTTTAACCA GCTGATGAAG 
CAAGTGTCAG GTCTTACTGT TGACACAGAG GAGCGGCTGA AAGGAGTCAT TGACCTGGTC 
TTTGAGAAGG CTATTGATGA ACCCAGTTTC TCTGTGGCTT ACGCAAACAT GTGTCGATGT 
CTAGTAACGC TGAAAGTACC CATGGCAGAC AAGCCTGGAA ACACAGTGAA TTTCCGGAAG 
CTGCTATTGA ACCGTTGCCA GAAGGAGTTT GAAAAAGATA AAGCAGATGA TGATGTCTTT 
GAGAAGAAAC AGAAAGAACT TGAGGCTGCC AGTGCTCCGG AGGAGAGGAC GAGGCTTCAC 
GACGAACTGG AAGAAGCTAA GGACAAAGCC CGGCGAAGAT CCATTGGTAA CATCAAGTTT 
ATTGGAGAAC TCTTTAAACT CAAAATGCTG ACGGAAGCTA TCATGCATGA CTGTGTGGTG 
AAGCTGCTAA AGAACCATGA TGAAGAATCC CTGGAGTGCC TCTGTCGCCT GCTCACCACC 
ATTGGCAAAG ACCTTGATTT TGAGAAAGCA AAGCCACGTA TGGACCAGTA TTTTAATCAG 
ATGGAGAAAA TCGTGAAAGA AAGGAAAACT TCATCTAGGA TTCGATTCAT GCTTCAGGAT 
GTGATAGACC TAAGACTGTG TAATTGGGTG TCTCGAAGAG CAGATCAAGG GCCTAAAACT 
ATTGAACAGA TTCACAAAGA GGCTAAAATA GAAGAACAAG AAGAGCAAAG GAAGGTCCAG 
CAGCTCATGA CCAAAGAGAA GAGAAGACCA GCAGGCGTCC AGAGAGTGGA TGAAGGTGGG 
TGGAACACTG TACAAGGGGC CAAGAATAGT CGGGTTCTGG ACCCCTCAAA ATTCCTGAAA 
ATCACTAAGC CTACAATTGA TGAGAAAATC CAGCTGGTAC CTAAAGCACA ACTGGGCAGT 
TGGGGAAAAG GCAGCAGTGG TGGAGCAAAG GCAAGCGAGA CTGATGCCTT AAGGTCAAGT 
GCTTCCAGTT TAAATAGATT CTCTGCTTTG CAACCTCCAG CACCCTCAGG GTCTACATCA 
TCCACACCTT TAGAGTTTGA TTCCCGCCGG ACCTTAACCA GTCGTGGAAG CATGGGCAGA 
GAGAAGAATG ACAAGCCCCT GCCATCTGCA GCAGCTCGTG CAAACACTTT CATGAGGGGC 
AGCAGTAGTA AAGACCTGTT AGACAATCAG TCTCAAGAAG AGCAACGGAG AGAGATGCTA 
GAGACGGTGA AACAGCTCAC CGGAGGCATG GATATGGACA GGAACAGCAC CGAGGCGGAC 
CGAAACAAAA CCAGGGAGTC AGGTGAGACA CCTTACGTGA ACAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023384898.1
CDS	702..4589
Translation	MNSQPQTRSPPSRTVPIHCTDNWKRRKVLEQTPVYRSLAGRGWIKYCIFAAGPRPAHHQFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLTMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIIVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQVGQMSETTAAESIPELPLPPSPTAISPIARSTIASPTAAARSSQPTFTTALDDRCELSSAKEDTVPIPSPTSCTETSDPSQTDVIDDDICKKSCSVAPNDIPLISSTNLINEMNGVSEKLPATESIVELVKQEVLPLTLELEILENPPEEMKVECVSAPITPSTVPSFSPSPPTPPASPPAPVIVPAAAMNVSTASAPATVQRVSEEDESIRTCLSEDAKEIQNKIEVEADGQTEETLDSQNLSSRKSPVLAQTAITAPKMWKKPKDRTRTTEEVLETELEPKAEEELSGDRVLESEQDKMSQGFHPERDSSDLKKVKTVEENGEEAEPVHNGAESVSEGEAIDANSGYTDSSGEGVTFSFKPESWKPADTEGKKQYDREFLLDFQFMPACIQKPEGLPPITDVVLDKINQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPICKVQSRHGLPILEQSKAPICTSLAMSHPPLKSLPLGLSNVGPRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQAEDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFEKDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPAGVQRVDEGGWNTVQGAKNSRVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKASETDALRSSASSLNRFSALQPPAPSGSTSSTPLEFDSRRTLTSRGSMGREKNDKPLPSAAARANTFMRGSSSKDLLDNQSQEEQRREMLETVKQLTGGMDMDRNSTEADRNKTRESGETPYVNR

Target ORF information:

RefSeq Version	XM_023529130.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023529130.1

ORF Insert Sequence:

ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA 
AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT 
TTCCAGAGGC CTCAAATACA GCCTCCTAGA GCTACCATCC CGAACAGCAG TCCTTCTATT 
CGTCCTGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT 
AACCATCTTA CCATGCCGTA CCCAGTACCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC 
CGTCATAGTG GCCCTCCATA TGTCGGACCC CCACAACAGT ATCCAGTTCA ACCACCGGGG 
CCAGGTCCTT TTTATCCTGG ACCAGGACCT GGGGACTTCC CCAATGCTTA CGGAACGCCT 
TTTTACCCAA GTCAGCCAGT GTATCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG 
CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GAGATCCAAA CCAGGGGGGT 
AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCA GAAATCCCAC TCCACCCATA 
GGGAGGCCCA CATCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC 
AGCCCTGTGG TTTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT 
GCAGCTAGCG ACCAGAAGCA AGAGGAGAAG CCAAAACCAG ACCCAGTGTT AAAGTCTCCT 
TCCCCAGTGC TTAGGTTAGT CCTTAGTGGA GAGAAGAAAG AACAAGTGGG CCAGATGTCT 
GAGACTACTG CAGCAGAATC CATACCAGAG CTTCCTCTGC CTCCATCACC AACCGCTATT 
TCTCCAATTG CTCGAAGTAC AATTGCTTCC CCCACCGCTG CTGCTCGCAG TAGCCAGCCG 
ACATTCACCA CTGCTCTAGA CGACAGATGT GAACTCTCCT CTGCAAAAGA AGACACAGTT 
CCTATACCCA GCCCCACATC TTGCACAGAA ACATCAGATC CTTCACAAAC AGATGTTATC 
GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCTA ATGATATTCC ACTGATTTCT 
AGTACTAACC TAATTAATGA AATGAATGGA GTTAGTGAAA AATTACCAGC CACGGAGAGC 
ATTGTGGAAT TAGTAAAACA GGAGGTGTTG CCACTGACTC TTGAATTGGA GATTCTGGAA 
AATCCCCCAG AAGAAATGAA AGTGGAGTGC GTTTCAGCTC CCATCACCCC TTCCACAGTT 
CCTTCCTTTT CTCCATCTCC TCCAACTCCT CCAGCTTCTC CTCCTGCGCC AGTCATTGTC 
CCTGCTGCTG CCATGAACGT TAGTACTGCT AGTGCTCCCG CCACAGTCCA GAGAGTCTCA 
GAAGAGGATG AGAGTATAAG AACTTGCCTC AGTGAAGATG CAAAAGAGAT TCAGAACAAA 
ATAGAGGTAG AAGCAGATGG GCAAACAGAA GAGACTTTGG ATTCTCAGAA CTTAAGTTCA 
AGAAAGAGCC CTGTCCTAGC TCAGACAGCT ATAACTGCAC CAAAGATGTG GAAGAAACCA 
AAAGATCGGA CCCGAACCAC TGAAGAGGTA TTAGAAACAG AATTGGAGCC TAAAGCTGAA 
GAGGAGCTTT CAGGTGACAG AGTACTTGAA TCTGAACAGG ATAAAATGAG CCAAGGGTTT 
CATCCTGAAA GAGACTCCTC AGATCTAAAA AAAGTGAAAA CTGTGGAAGA AAATGGAGAA 
GAAGCTGAGC CTGTACATAA TGGTGCTGAG AGTGTTTCTG AGGGTGAAGC AATAGATGCT 
AATTCAGGCT ACACTGATAG TTCTGGTGAA GGGGTCACAT TCTCATTTAA ACCAGAATCC 
TGGAAGCCTG CTGATACTGA AGGCAAGAAG CAGTATGACA GGGAGTTTTT ATTGGACTTT 
CAGTTCATGC CAGCCTGTAT ACAGAAACCA GAGGGCCTGC CTCCCATCAC TGATGTGGTT 
CTTGACAAGA TCAACCAACC CAAATTGCCA ATGCGAACTC TGGATCCTCG GATTTTGCCT 
CGAGGACCAG ATTTTACTCC AGCCTTTGCA GATTTTGGAA GGCAAACTCC TGGTGGTAGA 
GGTGTACCTA TTTGCAAAGT ACAGAGCAGG CATGGATTGC CAATTCTGGA ACAGAGCAAA 
GCCCCAATTT GCACCTCACT GGCGATGTCG CACCCACCCC TGAAGAGCCT GCCTCTAGGG 
TTGTCAAACG TTGGACCACG GAGATCTCAA CCTGGCCAGA GGAGAGAGCC CAGGAAGATC 
ATCACAGTCT CTGTAAAAGA AGACGTGCAC CTGAAAAAGG CAGAGAATGC CTGGAAGCCC 
AGCCAAAAAC GAGACAGCCA AGCTGAGGAC CCCGAAAACA TTAAAACCCA GGAACTTTTT 
AGAAAAGTTC GAAGTATCTT AAATAAGTTG ACACCACAGA TGTTTAACCA GCTGATGAAG 
CAAGTGTCAG GTCTTACTGT TGACACAGAG GAGCGGCTGA AAGGAGTCAT TGACCTGGTC 
TTTGAGAAGG CTATTGATGA ACCCAGTTTC TCTGTGGCTT ACGCAAACAT GTGTCGATGT 
CTAGTAACGC TGAAAGTACC CATGGCAGAC AAGCCTGGAA ACACAGTGAA TTTCCGGAAG 
CTGCTATTGA ACCGTTGCCA GAAGGAGTTT GAAAAAGATA AAGCAGATGA TGATGTCTTT 
GAGAAGAAAC AGAAAGAACT TGAGGCTGCC AGTGCTCCGG AGGAGAGGAC GAGGCTTCAC 
GACGAACTGG AAGAAGCTAA GGACAAAGCC CGGCGAAGAT CCATTGGTAA CATCAAGTTT 
ATTGGAGAAC TCTTTAAACT CAAAATGCTG ACGGAAGCTA TCATGCATGA CTGTGTGGTG 
AAGCTGCTAA AGAACCATGA TGAAGAATCC CTGGAGTGCC TCTGTCGCCT GCTCACCACC 
ATTGGCAAAG ACCTTGATTT TGAGAAAGCA AAGCCACGTA TGGACCAGTA TTTTAATCAG 
ATGGAGAAAA TCGTGAAAGA AAGGAAAACT TCATCTAGGA TTCGATTCAT GCTTCAGGAT 
GTGATAGACC TAAGACTGTG TAATTGGGTG TCTCGAAGAG CAGATCAAGG GCCTAAAACT 
ATTGAACAGA TTCACAAAGA GGCTAAAATA GAAGAACAAG AAGAGCAAAG GAAGGTCCAG 
CAGCTCATGA CCAAAGAGAA GAGAAGACCA GCAGGCGTCC AGAGAGTGGA TGAAGGTGGG 
TGGAACACTG TACAAGGGGC CAAGAATAGT CGGGTTCTGG ACCCCTCAAA ATTCCTGAAA 
ATCACTAAGC CTACAATTGA TGAGAAAATC CAGCTGGTAC CTAAAGCACA ACTGGGCAGT 
TGGGGAAAAG GCAGCAGTGG TGGAGCAAAG GCAAGCGAGA CTGATGCCTT AAGGTCAAGT 
GCTTCCAGTT TAAATAGATT CTCTGCTTTG CAACCTCCAG CACCCTCAGG GTCTACATCA 
TCCACACCTT TAGAGTTTGA TTCCCGCCGG ACCTTAACCA GTCGTGGAAG CATGGGCAGA 
GAGAAGAATG ACAAGCCCCT GCCATCTGCA GCAGCTCGTG CAAACACTTT CATGAGGGGC 
AGCAGTAGTA AAGACCTGTT AGACAATCAG TCTCAAGAAG AGCAACGGAG AGAGATGCTA 
GAGACGGTGA AACAGCTCAC CGGAGGCATG GATATGGACA GGAACAGCAC CGAGGCGGAC 
CGAAACAAAA CCAGGGAGTC AGGTGAGACA CCTTACGTGA ACAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G3 ( XM_011358019.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011356321.1 Pteropus vampyrus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), mRNA.

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CloneID	OPt45234
Clone ID Related Accession (Same CDS sequence)	XM_011358019.1
Accession Version	XM_011358019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	eukaryotic translation initiation factor 4 gamma 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888817.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA 
AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT 
TTCCAGAGGC CTCAAATACA GCCTCCTAGA GCTACCATCC CGAACAGCAG TCCTTCTATT 
CGTCCTGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT 
AACCATCTTA CCATGCCGTA CCCAGTACCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC 
CGTCATAGTG GCCCTCCATA TGTCGGACCC CCACAACAGT ATCCAGTTCA ACCACCGGGG 
CCAGGTCCTT TTTATCCTGG ACCAGGACCT GGGGACTTCC CCAATGCTTA CGGAACGCCT 
TTTTACCCAA GTCAGCCAGT GTATCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG 
CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GAGATCCAAA CCAGGGGGGT 
AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCA GAAATCCCAC TCCACCCATA 
GGGAGGCCCA CATCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC 
AGCCCTGTGG TTTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT 
GCAGCTAGCG ACCAGAAGCA AGAGGAGAAG CCAAAACCAG ACCCAGTGTT AAAGTCTCCT 
TCCCCAGTGC TTAGGTTAGT CCTTAGTGGA GAGAAGAAAG AACAAGTGGG CCAGATGTCT 
GAGACTACTG CAGCAGAATC CATACCAGAG CTTCCTCTGC CTCCATCACC AACCGCTATT 
TCTCCAATTG CTCGAAGTAC AATTGCTTCC CCCACCGCTG CTGCTCGCAG TAGCCAGCCG 
ACATTCACCA CTGCTCTAGA CGACAGATGT GAACTCTCCT CTGCAAAAGA AGACACAGTT 
CCTATACCCA GCCCCACATC TTGCACAGAA ACATCAGATC CTTCACAAAC AGATGTTATC 
GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCTA ATGATATTCC ACTGATTTCT 
AGTACTAACC TAATTAATGA AATGAATGGA GTTAGTGAAA AATTACCAGC CACGGAGAGC 
ATTGTGGAAT TAGTAAAACA GGAGGTGTTG CCACTGACTC TTGAATTGGA GATTCTGGAA 
AATCCCCCAG AAGAAATGAA AGTGGAGTGC GTTTCAGCTC CCATCACCCC TTCCACAGTT 
CCTTCCTTTT CTCCATCTCC TCCAACTCCT CCAGCTTCTC CTCCTGCGCC AGTCATTGTC 
CCTGCTGCTG CCATGAACGT TAGTACTGCT AGTGCTCCCG CCACAGTCCA GAGAGTCTCA 
GAAGAGGATG AGAGTATAAG AACTTGCCTC AGTGAAGATG CAAAAGAGAT TCAGAACAAA 
ATAGAGGTAG AAGCAGATGG GCAAACAGAA GAGACTTTGG ATTCTCAGAA CTTAAGTTCA 
AGAAAGAGCC CTGTCCTAGC TCAGACAGCT ATAACTGCAC CAAAGATGTG GAAGAAACCA 
AAAGATCGGA CCCGAACCAC TGAAGAGGTA TTAGAAACAG AATTGGAGCC TAAAGCTGAA 
GAGGAGCTTT CAGGTGACAG AGTACTTGAA TCTGAACAGG ATAAAATGAG CCAAGGGTTT 
CATCCTGAAA GAGACTCCTC AGATCTAAAA AAAGTGAAAA CTGTGGAAGA AAATGGAGAA 
GAAGCTGAGC CTGTACATAA TGGTGCTGAG AGTGTTTCTG AGGGTGAAGC AATAGATGCT 
AATTCAGGCT ACACTGATAG TTCTGGTGAA GGGGTCACAT TCTCATTTAA ACCAGAATCC 
TGGAAGCCTG CTGATACTGA AGGCAAGAAG CAGTATGACA GGGAGTTTTT ATTGGACTTT 
CAGTTCATGC CAGCCTGTAT ACAGAAACCA GAGGGCCTGC CTCCCATCAC TGATGTGGTT 
CTTGACAAGA TCAACCAACC CAAATTGCCA ATGCGAACTC TGGATCCTCG GATTTTGCCT 
CGAGGACCAG ATTTTACTCC AGCCTTTGCA GATTTTGGAA GGCAAACTCC TGGTGGTAGA 
GGTGTACCTA TTTGCAAAGT ACAGAGCAGG CATGGATTGC CAATTCTGGA ACAGAGCAAA 
GCCCCAATTT GCACCTCACT GGCGATGTCG CACCCACCCC TGAAGAGCCT GCCTCTAGGG 
TTGTCAAACG TTGGACCACG GAGATCTCAA CCTGGCCAGA GGAGAGAGCC CAGGAAGATC 
ATCACAGTCT CTGTAAAAGA AGACGTGCAC CTGAAAAAGG CAGAGAATGC CTGGAAGCCC 
AGCCAAAAAC GAGACAGCCA AGCTGAGGAC CCCGAAAACA TTAAAACCCA GGAACTTTTT 
AGAAAAGTTC GAAGTATCTT AAATAAGTTG ACACCACAGA TGTTTAACCA GCTGATGAAG 
CAAGTGTCAG GTCTTACTGT TGACACAGAG GAGCGGCTGA AAGGAGTCAT TGACCTGGTC 
TTTGAGAAGG CTATTGATGA ACCCAGTTTC TCTGTGGCTT ACGCAAACAT GTGTCGATGT 
CTAGTAACGC TGAAAGTACC CATGGCAGAC AAGCCTGGAA ACACAGTGAA TTTCCGGAAG 
CTGCTATTGA ACCGTTGCCA GAAGGAGTTT GAAAAAGATA AAGCAGATGA TGATGTCTTT 
GAGAAGAAAC AGAAAGAACT TGAGGCTGCC AGTGCTCCGG AGGAGAGGAC GAGGCTTCAC 
GACGAACTGG AAGAAGCTAA GGACAAAGCC CGGCGAAGAT CCATTGGTAA CATCAAGTTT 
ATTGGAGAAC TCTTTAAACT CAAAATGCTG ACGGAAGCTA TCATGCATGA CTGTGTGGTG 
AAGCTGCTAA AGAACCATGA TGAAGAATCC CTGGAGTGCC TCTGTCGCCT GCTCACCACC 
ATTGGCAAAG ACCTTGATTT TGAGAAAGCA AAGCCACGTA TGGACCAGTA TTTTAATCAG 
ATGGAGAAAA TCGTGAAAGA AAGGAAAACT TCATCTAGGA TTCGATTCAT GCTTCAGGAT 
GTGATAGACC TAAGACTGTG TAATTGGGTG TCTCGAAGAG CAGATCAAGG GCCTAAAACT 
ATTGAACAGA TTCACAAAGA GGCTAAAATA GAAGAACAAG AAGAGCAAAG GAAGGTCCAG 
CAGCTCATGA CCAAAGAGAA GAGAAGACCA GGCGTCCAGA GAGTGGATGA AGGTGGGTGG 
AACACTGTAC AAGGGGCCAA GAATAGTCGG GTTCTGGACC CCTCAAAATT CCTGAAAATC 
ACTAAGCCTA CAATTGATGA GAAAATCCAG CTGGTACCTA AAGCACAACT GGGCAGTTGG 
GGAAAAGGCA GCAGTGGTGG AGCAAAGGCA AGCGAGACTG ATGCCTTAAG GTCAAGTGCT 
TCCAGTTTAA ATAGATTCTC TGCTTTGCAA CCTCCAGCAC CCTCAGGGTC TACATCATCC 
ACACCTTTAG AGTTTGATTC CCGCCGGACC TTAACCAGTC GTGGAAGCAT GGGCAGAGAG 
AAGAATGACA AGCCCCTGCC ATCTGCAGCA GCTCGTGCAA ACACTTTCAT GAGGGGCAGC 
AGTAGTAAAG ACCTGTTAGA CAATCAGTCT CAAGAAGAGC AACGGAGAGA GATGCTAGAG 
ACGGTGAAAC AGCTCACCGG AGGCATGGAT ATGGACAGGA ACAGCACCGA GGCGGACCGA 
AACAAAACCA GGGAGTCAGC AAAACCAGAA ATTCCAGCAA CATCAGCCCC TGACAAGCCT 
GCATTGTCAG AAGAGGAGAT GGAGAGGAAG TCCAGATCTA TAATTGATGA ATTTCTACAC 
ATTAATGATT TTAAGGAAGC CATGCAGTGT GTGGAAGAGC TGAAGGCCCA GGATCTGCTA 
CATGTGTTTG TGAGAACAGG GGTGGAGTCC ACCCTGGAAA GGAGCCAAAT CACCAGGGAT 
CACATGGGCC AATTACTGTA TCAGCTGGTG CAGTCAGAAA AACTCAGCAA GCAAGACTTT 
TTCAAAGGTT TTTCAGAAAC ATTGGAATTG GCGGATGACA TGGCTATTGA TATTCCCCAT 
ATTTGGTTGT ACCTTGCCGA ATTGGTGACT CCCGTGTTAA AAGAAGGCGG AATCTCCATG 
AGAGAACTTA TCGTAGAATT TAGCAAACCT TTACTTCCTG TTGGAAGAGC TGGGGTCTTG 
CTTTCTGAAA TATTGCACCT TCTATGCAAA CAAATGAGCC ATAAGAAAGT GGGAGCCTTA 
TGGAGGGAAG CTGACCTCAG CTGGAAGGAC TTTTTACCAG AAGGGGAAGA TGTACATAAT 
TTTCTCTTGG AGCAGAAGTT GGACTTTATA GAGTCTGACA GTTCCTGTTC CTCCGAAGCA 
CTTTCAAAGA AAGAACTCTC TGCTGAAGAG CTATATAAGC GACTCGAGCA ACTCATCATT 
GAGGACAAAG CAAATGATGA ACAGATCTTT GACTGGGTAG AGGCTAATCT AGATGAAAGC 
CAGATGAGTT CACCTACATT CCTTAGAGCT TTAATGACAG CTGTTTGTAA AGCAGCTATT 
ATAGGCAATG CCCCAAAGCT GATGGTTCAT TTTCACTTCC ATACAGATTT GTTACGGATG 
TTTTTTGATT GCCTGTATGA TGAAGAGGTA ATCTCCGAGG ATGCCTTCTA CAAATGGGAA 
AGCAGCAAAG ACCCCGCAGA GCAGAATGGG AAGGGCGTGG CCCTCAAATC TGTCACGGCA 
TTCTTCACAT GGCTTCGGGA AGCAGAAGAG GAATCTGAGG ATAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011356321.1
CDS	685..5592
Translation	MNSQPQTRSPPSRTVPIHCTDNWKRRKVLEQTPVYRSLAGRGWIKYCIFAAGPRPAHHQFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLTMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIIVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQVGQMSETTAAESIPELPLPPSPTAISPIARSTIASPTAAARSSQPTFTTALDDRCELSSAKEDTVPIPSPTSCTETSDPSQTDVIDDDICKKSCSVAPNDIPLISSTNLINEMNGVSEKLPATESIVELVKQEVLPLTLELEILENPPEEMKVECVSAPITPSTVPSFSPSPPTPPASPPAPVIVPAAAMNVSTASAPATVQRVSEEDESIRTCLSEDAKEIQNKIEVEADGQTEETLDSQNLSSRKSPVLAQTAITAPKMWKKPKDRTRTTEEVLETELEPKAEEELSGDRVLESEQDKMSQGFHPERDSSDLKKVKTVEENGEEAEPVHNGAESVSEGEAIDANSGYTDSSGEGVTFSFKPESWKPADTEGKKQYDREFLLDFQFMPACIQKPEGLPPITDVVLDKINQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPICKVQSRHGLPILEQSKAPICTSLAMSHPPLKSLPLGLSNVGPRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQAEDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFEKDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRVDEGGWNTVQGAKNSRVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKASETDALRSSASSLNRFSALQPPAPSGSTSSTPLEFDSRRTLTSRGSMGREKNDKPLPSAAARANTFMRGSSSKDLLDNQSQEEQRREMLETVKQLTGGMDMDRNSTEADRNKTRESAKPEIPATSAPDKPALSEEEMERKSRSIIDEFLHINDFKEAMQCVEELKAQDLLHVFVRTGVESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPVLKEGGISMRELIVEFSKPLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREADLSWKDFLPEGEDVHNFLLEQKLDFIESDSSCSSEALSKKELSAEELYKRLEQLIIEDKANDEQIFDWVEANLDESQMSSPTFLRALMTAVCKAAIIGNAPKLMVHFHFHTDLLRMFFDCLYDEEVISEDAFYKWESSKDPAEQNGKGVALKSVTAFFTWLREAEEESEDN

Target ORF information:

RefSeq Version	XM_011358019.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011358019.1

ORF Insert Sequence:

ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA 
AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT 
TTCCAGAGGC CTCAAATACA GCCTCCTAGA GCTACCATCC CGAACAGCAG TCCTTCTATT 
CGTCCTGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT 
AACCATCTTA CCATGCCGTA CCCAGTACCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC 
CGTCATAGTG GCCCTCCATA TGTCGGACCC CCACAACAGT ATCCAGTTCA ACCACCGGGG 
CCAGGTCCTT TTTATCCTGG ACCAGGACCT GGGGACTTCC CCAATGCTTA CGGAACGCCT 
TTTTACCCAA GTCAGCCAGT GTATCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG 
CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GAGATCCAAA CCAGGGGGGT 
AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCA GAAATCCCAC TCCACCCATA 
GGGAGGCCCA CATCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC 
AGCCCTGTGG TTTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT 
GCAGCTAGCG ACCAGAAGCA AGAGGAGAAG CCAAAACCAG ACCCAGTGTT AAAGTCTCCT 
TCCCCAGTGC TTAGGTTAGT CCTTAGTGGA GAGAAGAAAG AACAAGTGGG CCAGATGTCT 
GAGACTACTG CAGCAGAATC CATACCAGAG CTTCCTCTGC CTCCATCACC AACCGCTATT 
TCTCCAATTG CTCGAAGTAC AATTGCTTCC CCCACCGCTG CTGCTCGCAG TAGCCAGCCG 
ACATTCACCA CTGCTCTAGA CGACAGATGT GAACTCTCCT CTGCAAAAGA AGACACAGTT 
CCTATACCCA GCCCCACATC TTGCACAGAA ACATCAGATC CTTCACAAAC AGATGTTATC 
GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCTA ATGATATTCC ACTGATTTCT 
AGTACTAACC TAATTAATGA AATGAATGGA GTTAGTGAAA AATTACCAGC CACGGAGAGC 
ATTGTGGAAT TAGTAAAACA GGAGGTGTTG CCACTGACTC TTGAATTGGA GATTCTGGAA 
AATCCCCCAG AAGAAATGAA AGTGGAGTGC GTTTCAGCTC CCATCACCCC TTCCACAGTT 
CCTTCCTTTT CTCCATCTCC TCCAACTCCT CCAGCTTCTC CTCCTGCGCC AGTCATTGTC 
CCTGCTGCTG CCATGAACGT TAGTACTGCT AGTGCTCCCG CCACAGTCCA GAGAGTCTCA 
GAAGAGGATG AGAGTATAAG AACTTGCCTC AGTGAAGATG CAAAAGAGAT TCAGAACAAA 
ATAGAGGTAG AAGCAGATGG GCAAACAGAA GAGACTTTGG ATTCTCAGAA CTTAAGTTCA 
AGAAAGAGCC CTGTCCTAGC TCAGACAGCT ATAACTGCAC CAAAGATGTG GAAGAAACCA 
AAAGATCGGA CCCGAACCAC TGAAGAGGTA TTAGAAACAG AATTGGAGCC TAAAGCTGAA 
GAGGAGCTTT CAGGTGACAG AGTACTTGAA TCTGAACAGG ATAAAATGAG CCAAGGGTTT 
CATCCTGAAA GAGACTCCTC AGATCTAAAA AAAGTGAAAA CTGTGGAAGA AAATGGAGAA 
GAAGCTGAGC CTGTACATAA TGGTGCTGAG AGTGTTTCTG AGGGTGAAGC AATAGATGCT 
AATTCAGGCT ACACTGATAG TTCTGGTGAA GGGGTCACAT TCTCATTTAA ACCAGAATCC 
TGGAAGCCTG CTGATACTGA AGGCAAGAAG CAGTATGACA GGGAGTTTTT ATTGGACTTT 
CAGTTCATGC CAGCCTGTAT ACAGAAACCA GAGGGCCTGC CTCCCATCAC TGATGTGGTT 
CTTGACAAGA TCAACCAACC CAAATTGCCA ATGCGAACTC TGGATCCTCG GATTTTGCCT 
CGAGGACCAG ATTTTACTCC AGCCTTTGCA GATTTTGGAA GGCAAACTCC TGGTGGTAGA 
GGTGTACCTA TTTGCAAAGT ACAGAGCAGG CATGGATTGC CAATTCTGGA ACAGAGCAAA 
GCCCCAATTT GCACCTCACT GGCGATGTCG CACCCACCCC TGAAGAGCCT GCCTCTAGGG 
TTGTCAAACG TTGGACCACG GAGATCTCAA CCTGGCCAGA GGAGAGAGCC CAGGAAGATC 
ATCACAGTCT CTGTAAAAGA AGACGTGCAC CTGAAAAAGG CAGAGAATGC CTGGAAGCCC 
AGCCAAAAAC GAGACAGCCA AGCTGAGGAC CCCGAAAACA TTAAAACCCA GGAACTTTTT 
AGAAAAGTTC GAAGTATCTT AAATAAGTTG ACACCACAGA TGTTTAACCA GCTGATGAAG 
CAAGTGTCAG GTCTTACTGT TGACACAGAG GAGCGGCTGA AAGGAGTCAT TGACCTGGTC 
TTTGAGAAGG CTATTGATGA ACCCAGTTTC TCTGTGGCTT ACGCAAACAT GTGTCGATGT 
CTAGTAACGC TGAAAGTACC CATGGCAGAC AAGCCTGGAA ACACAGTGAA TTTCCGGAAG 
CTGCTATTGA ACCGTTGCCA GAAGGAGTTT GAAAAAGATA AAGCAGATGA TGATGTCTTT 
GAGAAGAAAC AGAAAGAACT TGAGGCTGCC AGTGCTCCGG AGGAGAGGAC GAGGCTTCAC 
GACGAACTGG AAGAAGCTAA GGACAAAGCC CGGCGAAGAT CCATTGGTAA CATCAAGTTT 
ATTGGAGAAC TCTTTAAACT CAAAATGCTG ACGGAAGCTA TCATGCATGA CTGTGTGGTG 
AAGCTGCTAA AGAACCATGA TGAAGAATCC CTGGAGTGCC TCTGTCGCCT GCTCACCACC 
ATTGGCAAAG ACCTTGATTT TGAGAAAGCA AAGCCACGTA TGGACCAGTA TTTTAATCAG 
ATGGAGAAAA TCGTGAAAGA AAGGAAAACT TCATCTAGGA TTCGATTCAT GCTTCAGGAT 
GTGATAGACC TAAGACTGTG TAATTGGGTG TCTCGAAGAG CAGATCAAGG GCCTAAAACT 
ATTGAACAGA TTCACAAAGA GGCTAAAATA GAAGAACAAG AAGAGCAAAG GAAGGTCCAG 
CAGCTCATGA CCAAAGAGAA GAGAAGACCA GGCGTCCAGA GAGTGGATGA AGGTGGGTGG 
AACACTGTAC AAGGGGCCAA GAATAGTCGG GTTCTGGACC CCTCAAAATT CCTGAAAATC 
ACTAAGCCTA CAATTGATGA GAAAATCCAG CTGGTACCTA AAGCACAACT GGGCAGTTGG 
GGAAAAGGCA GCAGTGGTGG AGCAAAGGCA AGCGAGACTG ATGCCTTAAG GTCAAGTGCT 
TCCAGTTTAA ATAGATTCTC TGCTTTGCAA CCTCCAGCAC CCTCAGGGTC TACATCATCC 
ACACCTTTAG AGTTTGATTC CCGCCGGACC TTAACCAGTC GTGGAAGCAT GGGCAGAGAG 
AAGAATGACA AGCCCCTGCC ATCTGCAGCA GCTCGTGCAA ACACTTTCAT GAGGGGCAGC 
AGTAGTAAAG ACCTGTTAGA CAATCAGTCT CAAGAAGAGC AACGGAGAGA GATGCTAGAG 
ACGGTGAAAC AGCTCACCGG AGGCATGGAT ATGGACAGGA ACAGCACCGA GGCGGACCGA 
AACAAAACCA GGGAGTCAGC AAAACCAGAA ATTCCAGCAA CATCAGCCCC TGACAAGCCT 
GCATTGTCAG AAGAGGAGAT GGAGAGGAAG TCCAGATCTA TAATTGATGA ATTTCTACAC 
ATTAATGATT TTAAGGAAGC CATGCAGTGT GTGGAAGAGC TGAAGGCCCA GGATCTGCTA 
CATGTGTTTG TGAGAACAGG GGTGGAGTCC ACCCTGGAAA GGAGCCAAAT CACCAGGGAT 
CACATGGGCC AATTACTGTA TCAGCTGGTG CAGTCAGAAA AACTCAGCAA GCAAGACTTT 
TTCAAAGGTT TTTCAGAAAC ATTGGAATTG GCGGATGACA TGGCTATTGA TATTCCCCAT 
ATTTGGTTGT ACCTTGCCGA ATTGGTGACT CCCGTGTTAA AAGAAGGCGG AATCTCCATG 
AGAGAACTTA TCGTAGAATT TAGCAAACCT TTACTTCCTG TTGGAAGAGC TGGGGTCTTG 
CTTTCTGAAA TATTGCACCT TCTATGCAAA CAAATGAGCC ATAAGAAAGT GGGAGCCTTA 
TGGAGGGAAG CTGACCTCAG CTGGAAGGAC TTTTTACCAG AAGGGGAAGA TGTACATAAT 
TTTCTCTTGG AGCAGAAGTT GGACTTTATA GAGTCTGACA GTTCCTGTTC CTCCGAAGCA 
CTTTCAAAGA AAGAACTCTC TGCTGAAGAG CTATATAAGC GACTCGAGCA ACTCATCATT 
GAGGACAAAG CAAATGATGA ACAGATCTTT GACTGGGTAG AGGCTAATCT AGATGAAAGC 
CAGATGAGTT CACCTACATT CCTTAGAGCT TTAATGACAG CTGTTTGTAA AGCAGCTATT 
ATAGGCAATG CCCCAAAGCT GATGGTTCAT TTTCACTTCC ATACAGATTT GTTACGGATG 
TTTTTTGATT GCCTGTATGA TGAAGAGGTA ATCTCCGAGG ATGCCTTCTA CAAATGGGAA 
AGCAGCAAAG ACCCCGCAGA GCAGAATGGG AAGGGCGTGG CCCTCAAATC TGTCACGGCA 
TTCTTCACAT GGCTTCGGGA AGCAGAAGAG GAATCTGAGG ATAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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EIF4G3 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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