POPTR_0007s10880g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

Gene
Clones
gRNAs

Summary

Gene Symbol	POPTR_0007s10880g
Entrez Gene ID	7483107
Full Name	hypothetical protein
Synonyms	POPTRDRAFT_1083164
Gene Type	protein-coding
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024605512.1	XP_024461280.1	DNA polymerase alpha catalytic subunit isoform X1
XM_024605513.1	XP_024461281.1	DNA polymerase alpha catalytic subunit isoform X2
XM_002310683.3	XP_002310719.3	DNA polymerase alpha catalytic subunit isoform X1
XM_002310683.2	XP_002310719.2	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POPTR_0007s10880g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POPTR_0007s10880g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa17603	XM_002310683.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0007s10880g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPa433537	XM_024605512.1 Latest version!	Populus trichocarpa DNA polymerase alpha catalytic subunit (LOC7483107), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPa433537	XM_002310683.3 Latest version!	Populus trichocarpa DNA polymerase alpha catalytic subunit (LOC7483107), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPa433538	XM_024605513.1 Latest version!	Populus trichocarpa DNA polymerase alpha catalytic subunit (LOC7483107), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POPTR_0007s10880g ( XM_002310683.2 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002310719.2 Populus trichocarpa hypothetical protein (POPTR_0007s10880g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa17603
Clone ID Related Accession (Same CDS sequence)	XM_002310683.2
Accession Version	XM_002310683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008473). On Dec 16, 2013 this sequence version replaced XM_002310683.1. COMPLETENESS: incomplete on the 5' end.

ATGGCGGACG AGCAGCCAGC GGTGTTAAGT GGGAAGCGAA GAAGAGGAAC AGAAGCCACG 
GCCAGATCCG AAGCCCTTGA GCGACTCAAA GCGCTCCGTC GTGGTGGCAG GAGATCTGAA 
AATGGCGGCG GGTACGCCAT CAAAATGGAA GATCCAATCT ATGATTCCGT CCCCGAGGAC 
GAGTACAACA GTCTCGTCGC TCGCCGCCGA GAGGAAGCTC AGGGTTTCAT CGTTGACGAT 
GACGGCCTAG GTTACGGCGA CGAAGGTGAA GAAGAGGACT GGTCTCAGGC CGGATTACCT 
CCGTCTTCGG ATGAATCGGA CGGCGAGGGT TTGAATAAGA GTAGATCTAG AAAGAAGAAA 
AGTGAAAAGA AAGAGAAAGA GAAAGAGAAA GAAGTGAAGA AAGGTAATGC TTCGTTGACG 
GCTGCTGCGG CGGCGATTAT GGGGAAAACG AAGATATCGG CTATGTTTAC ATCAAATGTT 
TTTAAAAATA GGGATTCTGT TGGTAAGGTA AAGAGTTTTG ATTGTGACAA TATTGTTGAT 
GATGTTATCG CGGAGTTTGC TCCTGATGAA GCTGATAGAG AAAGACGTAG AAGAGGACAG 
TTGATTGTGA AGAATGCTAC ACCAATTAAG AGTGAAAATC GATTTTTTGA TAACGGTAAT 
GATGTTAATT TTATGGTTAA AGCAGATTTG GATAGGGATT TTATTAATTA TAAGAGTGGT 
GTTGATGAGA AAAAAGAGGG AATTGTGGAA GTGAAAGAGA AGGGAGAGGA TAAATTGTCT 
AATCTGGTTA AAGAGGAGGT TGTAAGTGTT TTGGAAGTGA AAGCAGACGA GGCAGTTGTG 
AAGAAGGATG GTGGACGTAC ACTTAATGCG AAGATTAGTG ATGAGGAGAG GGATCCAGCT 
TTGAGTGCTA CAGCCGGGTG GAAAGAAGTG ATGAATGGTA AGAATGGGGG TGAGGCTGTT 
GGTGGCGCTG GGGTAGTGGA GGAGGTTAAA AGTGGAGCAA ATTGTGAGGA GCAATCTGAG 
TTTGAGCTGG ATGGTGATGG GTCATTACCC TTTTACATTC TTGATGCTCA TGAAGAGATT 
TTTGGTGCTA ACAGGGGCAC GATCTATTTG TTTGGGAAGG TGAAAGCCGG AAATACATAT 
CATAGTTGCT GTGTGGTTGT AAAGAATATG CACAGATGTG TTTATGCAAT TCCAAATAGT 
TCTATATTTC GTACTGATGA GATGAGCATG CTAGAGAAAG AGGTTGAAGA GTCACGGATT 
TCTTCCACAG ATTTCCATAA AAAGCTGCAA GACATGGCAT ATGAACTAAA GAATGAAGTT 
GCAAGCCAAT TGTTAAGTCT CAATGTTTCA AGTTTCAGCA TGGCTCCGGT TAAGAGGAGA 
TATGCATTTG AGCGCTCTGA TATACCAGCT GGAGAGAATT ATGCACTCAA GATCAACTAT 
CCATTTAAGG AACCTCCGCT CCCAGCAGAT CTGAAAGGGG AAACTTTCTG TGCTTTGCTC 
GGAACTCATT GCAGTGCTTT AGAGCTTTTT CTCGTTAAAA GGAAGGTAAA GGGACCTTCG 
TGGCTTTCAG TTTCAAAATT TGCAACTTGC CCAGCTTCTC AAAAGGTGAG CTGGTGCAAG 
TTTGAGATCA TTGTCGAATC TCCAAAGGAA ATACAAGTTT CATCTTCATC AAATAGCAAA 
CTAGAGATTC CTCCAGTGGT TGTTGCTGCA ATAAATCTGA AAACTGTCAT AAATGAAAAG 
CAGAATGTGA ATGAAATAGT CTCTGCTTCT GTCATTTGCT GTCACAAGGC CAAGATTGAC 
ACTCCCATGT TGGCCTCCGA GTGGAAGAAA CCTGGCATGC TGAGCCATTT TACTGTTGTT 
CGCAAGCTTG ATGGAGGGAT ATTCCCAATG GGATTTTCCA AAGAGGTTAC AGACAGAAAC 
ACAATGGCTG GATCAAATGT CTTAGCTATT GAAAGCAGCG AAAGAGCTTT GTTAAATCGT 
CTGATGATTG CATTACACAA ATTAGACAGT GATTTTCTTG TTGGACATAA TATATCTGGT 
TTTGACCTGG ATGTTCTCCT CCATAGGACA CAGGCTTGCC GGGTGCCAAG TAGCACATGG 
TCCAAAATAG GACGTCTCAA GCGTTCTGTG ATGCCTAAAC TTACAAAAGG AAATGCCATT 
TTTGGCTCTG GAGCAAGTCC AGGGATCATG TCTTGCATTG CTGGCCGGCT CTTATGCGAT 
ACATATTTGG CTTCCCGTGA TTTATTAAAA GAGGTTAGTT ATTCCTTGAC GCAACTTGCT 
AAGACTCGGC TGAACAAAGA CCGCAAGGAG ATTGCACCAC ATGATATTCC CACAATGTTC 
CAGACGTCAA AGTCCCTTAT TGAACTAGTT GAATATGGTG AGACAGATGC ATGGTTATCT 
ATGGAACTCA TGTTTCATTT GAGCATTCTT CCTCTCACAC GCCAGCTGAC CAATATCAGT 
GGGAATCTCT GGGGGAAAAC TCTTCAAGGT GCGAGGGCAC AGAGAGTAGA GTATCTTCTA 
TTGCATGCAT TCCATGCAAA GAAATATATT GTCCCAGACA AGATGTCTTC TCGTGTAAAG 
GAAGCAAAAA TGACAAAGAG GAGAATTAAT AATGGCATTG AGGATAGAAA TGCTGATGAG 
TTGGACACTG ATGCTGCCAA TTTCGAAAAT GACAATCAAC AGAGTGATCA TGGGAAAAGA 
AAAAAAGGTC CTGCCTATGC AGGTGGATTG GTTTTGGAGC CTAAAAAAGG TTTATATGAC 
AAATATGTAT TGCTTCTGGA TTTCAATAGT CTTTACCCCT CAATTATTCA GGAATACAAT 
ATCTGCTTCA CAACAGTTGA AAGGTCTATG GATGGATTGG TTCCTCGTTT ACCTTCCAGT 
AAAACAACTG GAGTTCTGCC TGAGTTGCTG AAAAATTTAG TTGAGAGAAG AAGAATGGTT 
AAGTCATGGA TGAAGAATGC ATCTGGTCTC AAAGTCCAGC AGCTTGACAT TCAGCAACAA 
GCACTTAAGC TCACTGCAAA TAGTATGTAT GGATGCCTTG GGTTTTCCAA TTCAAGATTC 
TATGCAAAGC CACTTGCAGA ACTAATAACT CTACAAGGAA GGGAGATTTT ACAAAGTACT 
GTTGATCTTG TTCAGAACAA TTTGAACTTG GAGGTGATCT ATGGTGATAC AGATTCAATT 
ATGATTTATA GTGGACTAGA TGATATTCCA AAAGCAAAAG CTATTGCAGG GAAAGTCATC 
CAGGAGGTTA ACAAGAAGTA CAGGTGTCTA GAAATTGATC TTGATGGCTT GTACAAAAGA 
ATGCTTCTTC TAAAGAAGAA GAAATATGCA GCTGTTAAGG TGCAATTCAA GGATGGGACA 
CCATATGAGG TTATTGAACG TAAAGGTCTT GACATGGTTC GTCGTGACTG GAGCTTGCTA 
TCAAAGGAAT TGGGAGATTT CTGCTTGGCT CAAATTTTGT CTGGAGGGTC ATGCGAGGAC 
GTAATTGAAT CAATACATAA CTCCCTCATG AAGGTCCAGG AAGATATGAG GAGTGGACAA 
GTAGCCCTTG AGAAATATGT CATCACAAAG ACATTAACTA AACCACCTGA AGCCTATCCT 
GATGCCAAAA ATCAACCCCA TGCTCTGGTA GCACTAAGGT TGAAGCAAAG TGGTTATACA 
GCTGGTTGCT CTGCTGGTGA TACAGTACCG TATATAATTT GCTGTGAGCA GGGTGCGGGT 
GCTTCTGCAG TAAGTTTAAC AGGGATAGCC CAGCGTGCAA GACATCCTGA TGAATTGAAA 
TGTGATGATG GGAAATGGAT TATTGACATT GAGTACTACT TGTCGCAGCA AATTCATCCT 
GTGGTATCAC GTTTATGTGC TTCAATCCAG GGTACAAGCC CAGAGCGTTT GGCTGATTGC 
TTGGGGCTTG ACTCATCCAA GTTCCGTAGT AAATCAAGTG AATCTGTCCC TTCTAGCTCA 
CTCCTGTTTG CTGCTGATGA TGAGGAAAGA TATCAGAGTT GTGAGCCGCT AATCTTGTCA 
TGTCCCAGCT GCTCTGGTAC TTTTCACTGT CCTCCTGTCT TCATTTCCAT TTGTACGTCA 
ATTCTTGAGA AATCAAAAAA CCCACAAATC GAAGAATCTA TATCCAATTT CTGGCATAGA 
TTGTGTTGCC CTAAATGCCC AGAGGAAGGT GACTTGGGCA GAATATCTCC TGCAACGATG 
GCCAATCAGG TCAAAAGGCA AGCAGAGGGG TTTGTTTCCA CCTACTATAA AGGCGTCATG 
ATGTGTGATG ATGAAACTTG TAAGCATACC ACTAGGAGTC TCAACCTGAG GCTGGTTGGT 
GATTCCGAGA GAGGAACAGT TTGTCCAAGC TATCCACGCT GCAATGGGCG TCTAGTGAGA 
AAGTATACGG AAGCAGATTT GTACAAGCAA CTGTCGTATT TCTGTCACTT GCTCGACACT 
GCTCGCTGCA TTGAAAAGTT GCAGTCGGAC GCAGGCACTA GAATACAGGT AGAGAAGGAG 
TTGATGAAAA TCCGGCCAAT GGTTGATATG GCACTTTCAA CAGTTAAGAA GATTAGGGAC 
AGATGTGCTT ATGGGTGGGT GCAGTTGAAT GGCCTTGCAG TCACAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002310719.2
CDS	1..4611
Translation	MADEQPAVLSGKRRRGTEATARSEALERLKALRRGGRRSENGGGYAIKMEDPIYDSVPEDEYNSLVARRREEAQGFIVDDDGLGYGDEGEEEDWSQAGLPPSSDESDGEGLNKSRSRKKKSEKKEKEKEKEVKKGNASLTAAAAAIMGKTKISAMFTSNVFKNRDSVGKVKSFDCDNIVDDVIAEFAPDEADRERRRRGQLIVKNATPIKSENRFFDNGNDVNFMVKADLDRDFINYKSGVDEKKEGIVEVKEKGEDKLSNLVKEEVVSVLEVKADEAVVKKDGGRTLNAKISDEERDPALSATAGWKEVMNGKNGGEAVGGAGVVEEVKSGANCEEQSEFELDGDGSLPFYILDAHEEIFGANRGTIYLFGKVKAGNTYHSCCVVVKNMHRCVYAIPNSSIFRTDEMSMLEKEVEESRISSTDFHKKLQDMAYELKNEVASQLLSLNVSSFSMAPVKRRYAFERSDIPAGENYALKINYPFKEPPLPADLKGETFCALLGTHCSALELFLVKRKVKGPSWLSVSKFATCPASQKVSWCKFEIIVESPKEIQVSSSSNSKLEIPPVVVAAINLKTVINEKQNVNEIVSASVICCHKAKIDTPMLASEWKKPGMLSHFTVVRKLDGGIFPMGFSKEVTDRNTMAGSNVLAIESSERALLNRLMIALHKLDSDFLVGHNISGFDLDVLLHRTQACRVPSSTWSKIGRLKRSVMPKLTKGNAIFGSGASPGIMSCIAGRLLCDTYLASRDLLKEVSYSLTQLAKTRLNKDRKEIAPHDIPTMFQTSKSLIELVEYGETDAWLSMELMFHLSILPLTRQLTNISGNLWGKTLQGARAQRVEYLLLHAFHAKKYIVPDKMSSRVKEAKMTKRRINNGIEDRNADELDTDAANFENDNQQSDHGKRKKGPAYAGGLVLEPKKGLYDKYVLLLDFNSLYPSIIQEYNICFTTVERSMDGLVPRLPSSKTTGVLPELLKNLVERRRMVKSWMKNASGLKVQQLDIQQQALKLTANSMYGCLGFSNSRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDDIPKAKAIAGKVIQEVNKKYRCLEIDLDGLYKRMLLLKKKKYAAVKVQFKDGTPYEVIERKGLDMVRRDWSLLSKELGDFCLAQILSGGSCEDVIESIHNSLMKVQEDMRSGQVALEKYVITKTLTKPPEAYPDAKNQPHALVALRLKQSGYTAGCSAGDTVPYIICCEQGAGASAVSLTGIAQRARHPDELKCDDGKWIIDIEYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFRSKSSESVPSSSLLFAADDEERYQSCEPLILSCPSCSGTFHCPPVFISICTSILEKSKNPQIEESISNFWHRLCCPKCPEEGDLGRISPATMANQVKRQAEGFVSTYYKGVMMCDDETCKHTTRSLNLRLVGDSERGTVCPSYPRCNGRLVRKYTEADLYKQLSYFCHLLDTARCIEKLQSDAGTRIQVEKELMKIRPMVDMALSTVKKIRDRCAYGWVQLNGLAVTV

Target ORF information:

RefSeq Version	XM_002310683.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0007s10880g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002310683.2

ORF Insert Sequence:

ATGGCGGACG AGCAGCCAGC GGTGTTAAGT GGGAAGCGAA GAAGAGGAAC AGAAGCCACG 
GCCAGATCCG AAGCCCTTGA GCGACTCAAA GCGCTCCGTC GTGGTGGCAG GAGATCTGAA 
AATGGCGGCG GGTACGCCAT CAAAATGGAA GATCCAATCT ATGATTCCGT CCCCGAGGAC 
GAGTACAACA GTCTCGTCGC TCGCCGCCGA GAGGAAGCTC AGGGTTTCAT CGTTGACGAT 
GACGGCCTAG GTTACGGCGA CGAAGGTGAA GAAGAGGACT GGTCTCAGGC CGGATTACCT 
CCGTCTTCGG ATGAATCGGA CGGCGAGGGT TTGAATAAGA GTAGATCTAG AAAGAAGAAA 
AGTGAAAAGA AAGAGAAAGA GAAAGAGAAA GAAGTGAAGA AAGGTAATGC TTCGTTGACG 
GCTGCTGCGG CGGCGATTAT GGGGAAAACG AAGATATCGG CTATGTTTAC ATCAAATGTT 
TTTAAAAATA GGGATTCTGT TGGTAAGGTA AAGAGTTTTG ATTGTGACAA TATTGTTGAT 
GATGTTATCG CGGAGTTTGC TCCTGATGAA GCTGATAGAG AAAGACGTAG AAGAGGACAG 
TTGATTGTGA AGAATGCTAC ACCAATTAAG AGTGAAAATC GATTTTTTGA TAACGGTAAT 
GATGTTAATT TTATGGTTAA AGCAGATTTG GATAGGGATT TTATTAATTA TAAGAGTGGT 
GTTGATGAGA AAAAAGAGGG AATTGTGGAA GTGAAAGAGA AGGGAGAGGA TAAATTGTCT 
AATCTGGTTA AAGAGGAGGT TGTAAGTGTT TTGGAAGTGA AAGCAGACGA GGCAGTTGTG 
AAGAAGGATG GTGGACGTAC ACTTAATGCG AAGATTAGTG ATGAGGAGAG GGATCCAGCT 
TTGAGTGCTA CAGCCGGGTG GAAAGAAGTG ATGAATGGTA AGAATGGGGG TGAGGCTGTT 
GGTGGCGCTG GGGTAGTGGA GGAGGTTAAA AGTGGAGCAA ATTGTGAGGA GCAATCTGAG 
TTTGAGCTGG ATGGTGATGG GTCATTACCC TTTTACATTC TTGATGCTCA TGAAGAGATT 
TTTGGTGCTA ACAGGGGCAC GATCTATTTG TTTGGGAAGG TGAAAGCCGG AAATACATAT 
CATAGTTGCT GTGTGGTTGT AAAGAATATG CACAGATGTG TTTATGCAAT TCCAAATAGT 
TCTATATTTC GTACTGATGA GATGAGCATG CTAGAGAAAG AGGTTGAAGA GTCACGGATT 
TCTTCCACAG ATTTCCATAA AAAGCTGCAA GACATGGCAT ATGAACTAAA GAATGAAGTT 
GCAAGCCAAT TGTTAAGTCT CAATGTTTCA AGTTTCAGCA TGGCTCCGGT TAAGAGGAGA 
TATGCATTTG AGCGCTCTGA TATACCAGCT GGAGAGAATT ATGCACTCAA GATCAACTAT 
CCATTTAAGG AACCTCCGCT CCCAGCAGAT CTGAAAGGGG AAACTTTCTG TGCTTTGCTC 
GGAACTCATT GCAGTGCTTT AGAGCTTTTT CTCGTTAAAA GGAAGGTAAA GGGACCTTCG 
TGGCTTTCAG TTTCAAAATT TGCAACTTGC CCAGCTTCTC AAAAGGTGAG CTGGTGCAAG 
TTTGAGATCA TTGTCGAATC TCCAAAGGAA ATACAAGTTT CATCTTCATC AAATAGCAAA 
CTAGAGATTC CTCCAGTGGT TGTTGCTGCA ATAAATCTGA AAACTGTCAT AAATGAAAAG 
CAGAATGTGA ATGAAATAGT CTCTGCTTCT GTCATTTGCT GTCACAAGGC CAAGATTGAC 
ACTCCCATGT TGGCCTCCGA GTGGAAGAAA CCTGGCATGC TGAGCCATTT TACTGTTGTT 
CGCAAGCTTG ATGGAGGGAT ATTCCCAATG GGATTTTCCA AAGAGGTTAC AGACAGAAAC 
ACAATGGCTG GATCAAATGT CTTAGCTATT GAAAGCAGCG AAAGAGCTTT GTTAAATCGT 
CTGATGATTG CATTACACAA ATTAGACAGT GATTTTCTTG TTGGACATAA TATATCTGGT 
TTTGACCTGG ATGTTCTCCT CCATAGGACA CAGGCTTGCC GGGTGCCAAG TAGCACATGG 
TCCAAAATAG GACGTCTCAA GCGTTCTGTG ATGCCTAAAC TTACAAAAGG AAATGCCATT 
TTTGGCTCTG GAGCAAGTCC AGGGATCATG TCTTGCATTG CTGGCCGGCT CTTATGCGAT 
ACATATTTGG CTTCCCGTGA TTTATTAAAA GAGGTTAGTT ATTCCTTGAC GCAACTTGCT 
AAGACTCGGC TGAACAAAGA CCGCAAGGAG ATTGCACCAC ATGATATTCC CACAATGTTC 
CAGACGTCAA AGTCCCTTAT TGAACTAGTT GAATATGGTG AGACAGATGC ATGGTTATCT 
ATGGAACTCA TGTTTCATTT GAGCATTCTT CCTCTCACAC GCCAGCTGAC CAATATCAGT 
GGGAATCTCT GGGGGAAAAC TCTTCAAGGT GCGAGGGCAC AGAGAGTAGA GTATCTTCTA 
TTGCATGCAT TCCATGCAAA GAAATATATT GTCCCAGACA AGATGTCTTC TCGTGTAAAG 
GAAGCAAAAA TGACAAAGAG GAGAATTAAT AATGGCATTG AGGATAGAAA TGCTGATGAG 
TTGGACACTG ATGCTGCCAA TTTCGAAAAT GACAATCAAC AGAGTGATCA TGGGAAAAGA 
AAAAAAGGTC CTGCCTATGC AGGTGGATTG GTTTTGGAGC CTAAAAAAGG TTTATATGAC 
AAATATGTAT TGCTTCTGGA TTTCAATAGT CTTTACCCCT CAATTATTCA GGAATACAAT 
ATCTGCTTCA CAACAGTTGA AAGGTCTATG GATGGATTGG TTCCTCGTTT ACCTTCCAGT 
AAAACAACTG GAGTTCTGCC TGAGTTGCTG AAAAATTTAG TTGAGAGAAG AAGAATGGTT 
AAGTCATGGA TGAAGAATGC ATCTGGTCTC AAAGTCCAGC AGCTTGACAT TCAGCAACAA 
GCACTTAAGC TCACTGCAAA TAGTATGTAT GGATGCCTTG GGTTTTCCAA TTCAAGATTC 
TATGCAAAGC CACTTGCAGA ACTAATAACT CTACAAGGAA GGGAGATTTT ACAAAGTACT 
GTTGATCTTG TTCAGAACAA TTTGAACTTG GAGGTGATCT ATGGTGATAC AGATTCAATT 
ATGATTTATA GTGGACTAGA TGATATTCCA AAAGCAAAAG CTATTGCAGG GAAAGTCATC 
CAGGAGGTTA ACAAGAAGTA CAGGTGTCTA GAAATTGATC TTGATGGCTT GTACAAAAGA 
ATGCTTCTTC TAAAGAAGAA GAAATATGCA GCTGTTAAGG TGCAATTCAA GGATGGGACA 
CCATATGAGG TTATTGAACG TAAAGGTCTT GACATGGTTC GTCGTGACTG GAGCTTGCTA 
TCAAAGGAAT TGGGAGATTT CTGCTTGGCT CAAATTTTGT CTGGAGGGTC ATGCGAGGAC 
GTAATTGAAT CAATACATAA CTCCCTCATG AAGGTCCAGG AAGATATGAG GAGTGGACAA 
GTAGCCCTTG AGAAATATGT CATCACAAAG ACATTAACTA AACCACCTGA AGCCTATCCT 
GATGCCAAAA ATCAACCCCA TGCTCTGGTA GCACTAAGGT TGAAGCAAAG TGGTTATACA 
GCTGGTTGCT CTGCTGGTGA TACAGTACCG TATATAATTT GCTGTGAGCA GGGTGCGGGT 
GCTTCTGCAG TAAGTTTAAC AGGGATAGCC CAGCGTGCAA GACATCCTGA TGAATTGAAA 
TGTGATGATG GGAAATGGAT TATTGACATT GAGTACTACT TGTCGCAGCA AATTCATCCT 
GTGGTATCAC GTTTATGTGC TTCAATCCAG GGTACAAGCC CAGAGCGTTT GGCTGATTGC 
TTGGGGCTTG ACTCATCCAA GTTCCGTAGT AAATCAAGTG AATCTGTCCC TTCTAGCTCA 
CTCCTGTTTG CTGCTGATGA TGAGGAAAGA TATCAGAGTT GTGAGCCGCT AATCTTGTCA 
TGTCCCAGCT GCTCTGGTAC TTTTCACTGT CCTCCTGTCT TCATTTCCAT TTGTACGTCA 
ATTCTTGAGA AATCAAAAAA CCCACAAATC GAAGAATCTA TATCCAATTT CTGGCATAGA 
TTGTGTTGCC CTAAATGCCC AGAGGAAGGT GACTTGGGCA GAATATCTCC TGCAACGATG 
GCCAATCAGG TCAAAAGGCA AGCAGAGGGG TTTGTTTCCA CCTACTATAA AGGCGTCATG 
ATGTGTGATG ATGAAACTTG TAAGCATACC ACTAGGAGTC TCAACCTGAG GCTGGTTGGT 
GATTCCGAGA GAGGAACAGT TTGTCCAAGC TATCCACGCT GCAATGGGCG TCTAGTGAGA 
AAGTATACGG AAGCAGATTT GTACAAGCAA CTGTCGTATT TCTGTCACTT GCTCGACACT 
GCTCGCTGCA TTGAAAAGTT GCAGTCGGAC GCAGGCACTA GAATACAGGT AGAGAAGGAG 
TTGATGAAAA TCCGGCCAAT GGTTGATATG GCACTTTCAA CAGTTAAGAA GATTAGGGAC 
AGATGTGCTT ATGGGTGGGT GCAGTTGAAT GGCCTTGCAG TCACAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0007s10880g ( XM_024605512.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024461280.1 Populus trichocarpa DNA polymerase alpha catalytic subunit (LOC7483107), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa433537
Clone ID Related Accession (Same CDS sequence)	XM_024605512.1 , XM_002310683.3
Accession Version	XM_024605512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG AGCAGCCAGC GGTGTTAAGT GGGAAGCGAA GAAGAGGAAC AGAAGCCACG 
GCCAGATCCG AAGCCCTTGA GCGACTCAAA GCGCTCCGTC GTGGTGGCAG GAGATCTGAA 
AATGGCGGCG GGTACGCCAT CAAAATGGAA GATCCAATCT ATGATTCCGT CCCCGAGGAC 
GAGTACAACA GTCTCGTCGC TCGCCGCCGA GAGGAAGCTC AGGGTTTCAT CGTTGACGAT 
GACGGCCTAG GTTACGGCGA CGAAGGTGAA GAAGAGGACT GGTCTCAGGC CGGATTACCT 
CCGTCTTCGG ATGAATCGGA CGGCGAGGGT TTGAATAAGA GTAGATCTAG AAAGAAGAAA 
AGTGAAAAGA AAGAGAAAGA TAAAGAGAAA GAAGTGAAGA AAGGTAATGC TTCGTTGACG 
GCTGCTGCGG CGGCGATTAT GGGGAAAACG AAGATATCGG CTATGTTTAC ATCAAATGTT 
TTTAAAAATA GGGATTCTGT TGGTAAGGTA AAGAGTTTTG ACTGTGACAA TATTGTTGAT 
GATGTTATCG CGGAGTTTGC TCCTGATGAA GCTGATAGAG AAAGGCGTAG AAGAGGACAG 
TTGATTGTGA AGAATGCTAC ACCAATTAAG AGTGAAAATC GATTTTTTGA TAACGGTAAT 
GATGTTAATT TTATGGTTAA AGCAGATTTG GATAGGGATT TTATTAATTT TAAGAGTGGT 
GTTGATGAGA AAAAAGAGGG AATTGTGGAA GTGAAAGAGA AGGGAGAGGA TAAATTGTCT 
AATCTGGTTA AAGAGGAGGT TGTAAGTGTT TTGGAAGTGA AAGCAGACGA GGCAGTTGTG 
AAGAAGGATG GTGGACGTAC ACTTAATGCG AAGATTAGTG ATGAGGAGAG GGATCCAGCT 
TTGAGTGCTA CAGCCGGGTG GAAAGAAGTG ATGAATGGTA AGAATGGGGG TGAGGCTGTT 
GGTGGCGCTG GGGTAGTGGA GGAGGTTAAA AGTGGAGCAA ATTGTGAGGA GCAATCTGAG 
TTTGAGCTGG ATGGTGACGG GTCATTACCC TTTTACATTC TTGATGCTCA TGAAGAGATT 
TTTGGTGCTA ACACGGGCAC GATCTATTTG TTTGGGAAGG TGAAAGCCGG AAATACATAT 
CATAGTTGCT GTGTGGTTGT AAAGAATATG CACAGATGTG TTTATGCAAT TCCAAATAGT 
TCTATATTTC GTACTGATGA GATGAGCATG CTAGAGAAAG AGGTTGAAGA GTCACGGATT 
TCTTCCACAG ATTTCCATAA AAAGCTGCAA GACATGGCAT ATGAACTAAA GAATGAAGTT 
GCAAGCCAAT TGTTAAGTCT CAATGTTTCA AGTTTCAGCA TGGCTCCGGT TAAGAGGAGA 
TATGCATTTG ACCGCTCTGA TATACCAGCT GGAGAGAATT ATGCACTCAA GATCAACTAT 
CCATTTAAGG AACCTCCGCT CCCAGCAGAT CTGAAAGGGG AAACTTTCTG TGCTTTGCTC 
GGAACTCATT GCAGTGCTTT AGAGCTTTTT CTCGTTAAAA GGAAGGTAAA GGGACCTTCG 
TGGCTTTCAG TTTCAAAATT TGCAACTTGC CCAGCTTCTC AAAAGGTGAG CTGGTGCAAG 
TTTGAGATCA TTGTCGAATC TCCAAAGGAC ATACAAGTTT CATCTTCATC AAATAGCAAA 
ATAGAGATTC CTCCAGTGGT TGTTGCTGCA ATAAATCTGA AAACTGTCAT AAATGAAAAG 
CAGAATGTGA ATGAAATAGT CTCTGCTTCT GTCATTTGCT GTCACAAGGC CAAGATTGAC 
ACTCCCATGT TGGCCTCCGA GTGGAAGAAA CCTGGCATGC TGAGCCATTT TACTGTTGTT 
CGCAAGCTTG ATGGAGGGAT ATTCCCAATG GGATTTTCCA AAGAGGTTAC AGACAGAAAC 
ACAATGGCTG GATCAAATGT CTTAGCTATT GAAAGCAGCG AAAGAGCTTT GTTAAATCGT 
CTGATGATTG CATTACACAA ATTAGACAGT GATTTTCTTG TTGGACATAA TATATCTGGT 
TTTGACCTGG ATGTTCTCCT CCATAGGACA CAGGCTTGCC GGGTGCCAAG TAGCACATGG 
TCCAAAATAG GACGTCTCAA GCGTTCTGTG ATGCCTAAAC TTACAAAAGG AAATGCCATT 
TTTGGCTCTG GAGCAAGTCC AGGGATCATG TCTTGCATTG CTGGCCGGCT CTTATGCGAT 
ACATATTTGG CTTCCCGTGA TTTATTAAAA GAGGTTAGTT ATTCCTTGAC GCAACTTGCT 
AAGACTCGGC TGAACAAAGA CCGCAAGGAG ATTGCACCAC ATGATATTCC CACAATGTTC 
CAGACGTCAA AGTCCCTTAT TGAACTAGTT GAATATGGTG AGACAGATGC ATGGTTATCT 
ATGGAACTCA TGTTTCATTT GAGCATTCTT CCTCTCACAC GCCAGCTGAC CAATATCAGT 
GGGAATCTCT GGGGGAAAAC TCTTCAAGGT GCGAGGGCAC AGAGAGTAGA GTATCTTCTA 
TTGCATGCAT TCCATGCAAA GAAATATATT GTCCCAGACA AGATGTCTTC TCGTGTAAAG 
GAAGCAAAAA TAACAAAGAG GAGAATTAAT AATGGCATTG AGGATAGAAA TGCTGATGAG 
TTGGACACTG ATGCTGCCAA TTTCGAAAAT GACAATCAAC AGAGTGATCA TGGGAAAAGA 
AAAAAAGGTC CTGCCTATGC AGGTGGATTG GTTTTGGAGC CTAAAAAAGG TTTATATGAC 
AAATATGTAT TGCTTCTGGA TTTCAATAGT CTTTACCCCT CAATTATTCA GGAATACAAT 
ATCTGCTTCA CAACAGTTGA AAGGTCTATG GATGGATTGG TTCCTCGTTT ACCTTCCAGT 
AAAACAACTG GAGTTCTGCC TGAGTTGCTG AAAAATTTAG TTGAGAGAAG AAGAATGGTT 
AAGTCATGGA TGAAGAATGC ATCTGGTCTC AAAGTCCAGC AGCTTGACAT TCAGCAACAA 
GCACTTAAGC TCACTGCAAA TAGTATGTAT GGATGCCTTG GGTTTTCCAA TTCAAGATTC 
TATGCAAAGC CACTTGCAGA ACTAATAACT CTACAAGGAA GGGAGATTTT ACAAAGTACT 
GTTGATCTTG TTCAGAACAA TTTGAACTTG GAGGTGATCT ATGGTGATAC AGATTCAATT 
ATGATTTATA GTGGACTAGA TGATATTCCA AAAGCAAAAG CTATTGCAGG GAAAGTCATC 
CAGGAGGTTA ACAAGAAGTA CAGGTGTCTA GAAATTGATC TTGATGGCTT GTACAAAAGA 
ATGCTTCTTC TAAAGAAGAA GAAATATGCA GCTGTTAAGG TGCAATTCAA GGATGGGACA 
CCATATGAGG TTATTGAACG TAAAGGTCTT GACATGGTTC GTCGTGACTG GAGCTTGCTA 
TCAAAGGAAT TGGGAGATTT CTGCCTGGCT CAAATTTTGT CTGGAGGGTC ATGCGAGGAC 
GTAATTGAAT CAATACATAA CTCCCTCATG AAGGTCCAGG AAGATATGAG GAGTGGACAA 
GTAGCCCTTG AGAAATATGT CATCACAAAG ACATTAACTA AACCACCTGA AGCCTATCCT 
GATGCCAAAA ATCAACCCCA TGCTCTGGTA GCACTAAGGT TGAAGCAAAG TGGTTATACA 
GCTGGTTGCT CTGCTGGTGA TACAGTACCG TATATAATTT GCTGTGAGCA GGGTGCGGGT 
GCTTCTGCAG TAAGTTTAAC AGGGATAGCC CAGCGTGCAA GACATCCTGA TGAATTGAAA 
TGTGATGATG GGAAATGGAT TATTGACATT GAGTACTACT TGTCACAGCA AATTCATCCT 
GTGGTATCAC GTTTATGTGC TTCAATCCAG GGTACAAGCC CAGAGCGTTT GGCTGATTGC 
TTGGGGCTTG ACTCATCCAA GTTCCGTAGT AAATCAAGTG AATCTGTCCC TTCTAGCTCA 
CTCCTGTTTG CTGCTGATGA TGAGGAAAGA TATCAGAGTT GTGAGCCGCT AATCTTGTCA 
TGTCCCAGCT GCTCTGGTAC TTTTCACTGT CCTCCTGTCT TCATTTCCAT TTGTACGTCA 
ATTCTTGAGA AATCAAAAAA CCCACAAATC GAAGAATCTA TATCCAATTT CTGGCATAGA 
TTGTGTTGCC CTAAATGCCC AGAGGAAGGT GACTTGGGCA GAATATCTCC TGCAACGATG 
GCCAATCAGG TCAAAAGGCA AGCAGAGGGG TTTGTTTCCA CCTACTATAA AGGCGTCATG 
ATGTGTGATG ATGAAACTTG TAAGCATACC ACTAGGAGTC TCAACCTGAG GCTGGTTGGT 
GATTCCGAGA GAGGAACAGT TTGTCCAAGC TATCCACGCT GCAATGGGCG TCTAGTGAGA 
AAGTATACGG AAGCAGATTT GTACAAGCAA CTGTCGTATT TCTGTCACTT GCTCGAGACT 
GCTCGCTGCA TTGAAAAGTT GCAGGCGGAC GCAGGCACTA GAATACAGGT AGAGAAGGAG 
TTGATGAAAA TCCGGCCAAT GGTTGATATG GCACTTTCAA CAGTTAAGAA GATTAGGGAC 
AGATGTGCTT ATGGGTGGGT GCAGTTGAAT GGCCTTGCAG TCACAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024461280.1
CDS	118..4728
Translation	MADEQPAVLSGKRRRGTEATARSEALERLKALRRGGRRSENGGGYAIKMEDPIYDSVPEDEYNSLVARRREEAQGFIVDDDGLGYGDEGEEEDWSQAGLPPSSDESDGEGLNKSRSRKKKSEKKEKDKEKEVKKGNASLTAAAAAIMGKTKISAMFTSNVFKNRDSVGKVKSFDCDNIVDDVIAEFAPDEADRERRRRGQLIVKNATPIKSENRFFDNGNDVNFMVKADLDRDFINFKSGVDEKKEGIVEVKEKGEDKLSNLVKEEVVSVLEVKADEAVVKKDGGRTLNAKISDEERDPALSATAGWKEVMNGKNGGEAVGGAGVVEEVKSGANCEEQSEFELDGDGSLPFYILDAHEEIFGANTGTIYLFGKVKAGNTYHSCCVVVKNMHRCVYAIPNSSIFRTDEMSMLEKEVEESRISSTDFHKKLQDMAYELKNEVASQLLSLNVSSFSMAPVKRRYAFDRSDIPAGENYALKINYPFKEPPLPADLKGETFCALLGTHCSALELFLVKRKVKGPSWLSVSKFATCPASQKVSWCKFEIIVESPKDIQVSSSSNSKIEIPPVVVAAINLKTVINEKQNVNEIVSASVICCHKAKIDTPMLASEWKKPGMLSHFTVVRKLDGGIFPMGFSKEVTDRNTMAGSNVLAIESSERALLNRLMIALHKLDSDFLVGHNISGFDLDVLLHRTQACRVPSSTWSKIGRLKRSVMPKLTKGNAIFGSGASPGIMSCIAGRLLCDTYLASRDLLKEVSYSLTQLAKTRLNKDRKEIAPHDIPTMFQTSKSLIELVEYGETDAWLSMELMFHLSILPLTRQLTNISGNLWGKTLQGARAQRVEYLLLHAFHAKKYIVPDKMSSRVKEAKITKRRINNGIEDRNADELDTDAANFENDNQQSDHGKRKKGPAYAGGLVLEPKKGLYDKYVLLLDFNSLYPSIIQEYNICFTTVERSMDGLVPRLPSSKTTGVLPELLKNLVERRRMVKSWMKNASGLKVQQLDIQQQALKLTANSMYGCLGFSNSRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDDIPKAKAIAGKVIQEVNKKYRCLEIDLDGLYKRMLLLKKKKYAAVKVQFKDGTPYEVIERKGLDMVRRDWSLLSKELGDFCLAQILSGGSCEDVIESIHNSLMKVQEDMRSGQVALEKYVITKTLTKPPEAYPDAKNQPHALVALRLKQSGYTAGCSAGDTVPYIICCEQGAGASAVSLTGIAQRARHPDELKCDDGKWIIDIEYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFRSKSSESVPSSSLLFAADDEERYQSCEPLILSCPSCSGTFHCPPVFISICTSILEKSKNPQIEESISNFWHRLCCPKCPEEGDLGRISPATMANQVKRQAEGFVSTYYKGVMMCDDETCKHTTRSLNLRLVGDSERGTVCPSYPRCNGRLVRKYTEADLYKQLSYFCHLLETARCIEKLQADAGTRIQVEKELMKIRPMVDMALSTVKKIRDRCAYGWVQLNGLAVTV

Target ORF information:

RefSeq Version	XM_024605512.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa DNA polymerase alpha catalytic subunit (LOC7483107), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024605512.1

ORF Insert Sequence:

ATGGCGGACG AGCAGCCAGC GGTGTTAAGT GGGAAGCGAA GAAGAGGAAC AGAAGCCACG 
GCCAGATCCG AAGCCCTTGA GCGACTCAAA GCGCTCCGTC GTGGTGGCAG GAGATCTGAA 
AATGGCGGCG GGTACGCCAT CAAAATGGAA GATCCAATCT ATGATTCCGT CCCCGAGGAC 
GAGTACAACA GTCTCGTCGC TCGCCGCCGA GAGGAAGCTC AGGGTTTCAT CGTTGACGAT 
GACGGCCTAG GTTACGGCGA CGAAGGTGAA GAAGAGGACT GGTCTCAGGC CGGATTACCT 
CCGTCTTCGG ATGAATCGGA CGGCGAGGGT TTGAATAAGA GTAGATCTAG AAAGAAGAAA 
AGTGAAAAGA AAGAGAAAGA TAAAGAGAAA GAAGTGAAGA AAGGTAATGC TTCGTTGACG 
GCTGCTGCGG CGGCGATTAT GGGGAAAACG AAGATATCGG CTATGTTTAC ATCAAATGTT 
TTTAAAAATA GGGATTCTGT TGGTAAGGTA AAGAGTTTTG ACTGTGACAA TATTGTTGAT 
GATGTTATCG CGGAGTTTGC TCCTGATGAA GCTGATAGAG AAAGGCGTAG AAGAGGACAG 
TTGATTGTGA AGAATGCTAC ACCAATTAAG AGTGAAAATC GATTTTTTGA TAACGGTAAT 
GATGTTAATT TTATGGTTAA AGCAGATTTG GATAGGGATT TTATTAATTT TAAGAGTGGT 
GTTGATGAGA AAAAAGAGGG AATTGTGGAA GTGAAAGAGA AGGGAGAGGA TAAATTGTCT 
AATCTGGTTA AAGAGGAGGT TGTAAGTGTT TTGGAAGTGA AAGCAGACGA GGCAGTTGTG 
AAGAAGGATG GTGGACGTAC ACTTAATGCG AAGATTAGTG ATGAGGAGAG GGATCCAGCT 
TTGAGTGCTA CAGCCGGGTG GAAAGAAGTG ATGAATGGTA AGAATGGGGG TGAGGCTGTT 
GGTGGCGCTG GGGTAGTGGA GGAGGTTAAA AGTGGAGCAA ATTGTGAGGA GCAATCTGAG 
TTTGAGCTGG ATGGTGACGG GTCATTACCC TTTTACATTC TTGATGCTCA TGAAGAGATT 
TTTGGTGCTA ACACGGGCAC GATCTATTTG TTTGGGAAGG TGAAAGCCGG AAATACATAT 
CATAGTTGCT GTGTGGTTGT AAAGAATATG CACAGATGTG TTTATGCAAT TCCAAATAGT 
TCTATATTTC GTACTGATGA GATGAGCATG CTAGAGAAAG AGGTTGAAGA GTCACGGATT 
TCTTCCACAG ATTTCCATAA AAAGCTGCAA GACATGGCAT ATGAACTAAA GAATGAAGTT 
GCAAGCCAAT TGTTAAGTCT CAATGTTTCA AGTTTCAGCA TGGCTCCGGT TAAGAGGAGA 
TATGCATTTG ACCGCTCTGA TATACCAGCT GGAGAGAATT ATGCACTCAA GATCAACTAT 
CCATTTAAGG AACCTCCGCT CCCAGCAGAT CTGAAAGGGG AAACTTTCTG TGCTTTGCTC 
GGAACTCATT GCAGTGCTTT AGAGCTTTTT CTCGTTAAAA GGAAGGTAAA GGGACCTTCG 
TGGCTTTCAG TTTCAAAATT TGCAACTTGC CCAGCTTCTC AAAAGGTGAG CTGGTGCAAG 
TTTGAGATCA TTGTCGAATC TCCAAAGGAC ATACAAGTTT CATCTTCATC AAATAGCAAA 
ATAGAGATTC CTCCAGTGGT TGTTGCTGCA ATAAATCTGA AAACTGTCAT AAATGAAAAG 
CAGAATGTGA ATGAAATAGT CTCTGCTTCT GTCATTTGCT GTCACAAGGC CAAGATTGAC 
ACTCCCATGT TGGCCTCCGA GTGGAAGAAA CCTGGCATGC TGAGCCATTT TACTGTTGTT 
CGCAAGCTTG ATGGAGGGAT ATTCCCAATG GGATTTTCCA AAGAGGTTAC AGACAGAAAC 
ACAATGGCTG GATCAAATGT CTTAGCTATT GAAAGCAGCG AAAGAGCTTT GTTAAATCGT 
CTGATGATTG CATTACACAA ATTAGACAGT GATTTTCTTG TTGGACATAA TATATCTGGT 
TTTGACCTGG ATGTTCTCCT CCATAGGACA CAGGCTTGCC GGGTGCCAAG TAGCACATGG 
TCCAAAATAG GACGTCTCAA GCGTTCTGTG ATGCCTAAAC TTACAAAAGG AAATGCCATT 
TTTGGCTCTG GAGCAAGTCC AGGGATCATG TCTTGCATTG CTGGCCGGCT CTTATGCGAT 
ACATATTTGG CTTCCCGTGA TTTATTAAAA GAGGTTAGTT ATTCCTTGAC GCAACTTGCT 
AAGACTCGGC TGAACAAAGA CCGCAAGGAG ATTGCACCAC ATGATATTCC CACAATGTTC 
CAGACGTCAA AGTCCCTTAT TGAACTAGTT GAATATGGTG AGACAGATGC ATGGTTATCT 
ATGGAACTCA TGTTTCATTT GAGCATTCTT CCTCTCACAC GCCAGCTGAC CAATATCAGT 
GGGAATCTCT GGGGGAAAAC TCTTCAAGGT GCGAGGGCAC AGAGAGTAGA GTATCTTCTA 
TTGCATGCAT TCCATGCAAA GAAATATATT GTCCCAGACA AGATGTCTTC TCGTGTAAAG 
GAAGCAAAAA TAACAAAGAG GAGAATTAAT AATGGCATTG AGGATAGAAA TGCTGATGAG 
TTGGACACTG ATGCTGCCAA TTTCGAAAAT GACAATCAAC AGAGTGATCA TGGGAAAAGA 
AAAAAAGGTC CTGCCTATGC AGGTGGATTG GTTTTGGAGC CTAAAAAAGG TTTATATGAC 
AAATATGTAT TGCTTCTGGA TTTCAATAGT CTTTACCCCT CAATTATTCA GGAATACAAT 
ATCTGCTTCA CAACAGTTGA AAGGTCTATG GATGGATTGG TTCCTCGTTT ACCTTCCAGT 
AAAACAACTG GAGTTCTGCC TGAGTTGCTG AAAAATTTAG TTGAGAGAAG AAGAATGGTT 
AAGTCATGGA TGAAGAATGC ATCTGGTCTC AAAGTCCAGC AGCTTGACAT TCAGCAACAA 
GCACTTAAGC TCACTGCAAA TAGTATGTAT GGATGCCTTG GGTTTTCCAA TTCAAGATTC 
TATGCAAAGC CACTTGCAGA ACTAATAACT CTACAAGGAA GGGAGATTTT ACAAAGTACT 
GTTGATCTTG TTCAGAACAA TTTGAACTTG GAGGTGATCT ATGGTGATAC AGATTCAATT 
ATGATTTATA GTGGACTAGA TGATATTCCA AAAGCAAAAG CTATTGCAGG GAAAGTCATC 
CAGGAGGTTA ACAAGAAGTA CAGGTGTCTA GAAATTGATC TTGATGGCTT GTACAAAAGA 
ATGCTTCTTC TAAAGAAGAA GAAATATGCA GCTGTTAAGG TGCAATTCAA GGATGGGACA 
CCATATGAGG TTATTGAACG TAAAGGTCTT GACATGGTTC GTCGTGACTG GAGCTTGCTA 
TCAAAGGAAT TGGGAGATTT CTGCCTGGCT CAAATTTTGT CTGGAGGGTC ATGCGAGGAC 
GTAATTGAAT CAATACATAA CTCCCTCATG AAGGTCCAGG AAGATATGAG GAGTGGACAA 
GTAGCCCTTG AGAAATATGT CATCACAAAG ACATTAACTA AACCACCTGA AGCCTATCCT 
GATGCCAAAA ATCAACCCCA TGCTCTGGTA GCACTAAGGT TGAAGCAAAG TGGTTATACA 
GCTGGTTGCT CTGCTGGTGA TACAGTACCG TATATAATTT GCTGTGAGCA GGGTGCGGGT 
GCTTCTGCAG TAAGTTTAAC AGGGATAGCC CAGCGTGCAA GACATCCTGA TGAATTGAAA 
TGTGATGATG GGAAATGGAT TATTGACATT GAGTACTACT TGTCACAGCA AATTCATCCT 
GTGGTATCAC GTTTATGTGC TTCAATCCAG GGTACAAGCC CAGAGCGTTT GGCTGATTGC 
TTGGGGCTTG ACTCATCCAA GTTCCGTAGT AAATCAAGTG AATCTGTCCC TTCTAGCTCA 
CTCCTGTTTG CTGCTGATGA TGAGGAAAGA TATCAGAGTT GTGAGCCGCT AATCTTGTCA 
TGTCCCAGCT GCTCTGGTAC TTTTCACTGT CCTCCTGTCT TCATTTCCAT TTGTACGTCA 
ATTCTTGAGA AATCAAAAAA CCCACAAATC GAAGAATCTA TATCCAATTT CTGGCATAGA 
TTGTGTTGCC CTAAATGCCC AGAGGAAGGT GACTTGGGCA GAATATCTCC TGCAACGATG 
GCCAATCAGG TCAAAAGGCA AGCAGAGGGG TTTGTTTCCA CCTACTATAA AGGCGTCATG 
ATGTGTGATG ATGAAACTTG TAAGCATACC ACTAGGAGTC TCAACCTGAG GCTGGTTGGT 
GATTCCGAGA GAGGAACAGT TTGTCCAAGC TATCCACGCT GCAATGGGCG TCTAGTGAGA 
AAGTATACGG AAGCAGATTT GTACAAGCAA CTGTCGTATT TCTGTCACTT GCTCGAGACT 
GCTCGCTGCA TTGAAAAGTT GCAGGCGGAC GCAGGCACTA GAATACAGGT AGAGAAGGAG 
TTGATGAAAA TCCGGCCAAT GGTTGATATG GCACTTTCAA CAGTTAAGAA GATTAGGGAC 
AGATGTGCTT ATGGGTGGGT GCAGTTGAAT GGCCTTGCAG TCACAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0007s10880g ( XM_002310683.3 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002310719.3 Populus trichocarpa DNA polymerase alpha catalytic subunit (LOC7483107), transcript variant X1, mRNA.

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CloneID	OPa433537
Clone ID Related Accession (Same CDS sequence)	XM_024605512.1 , XM_002310683.3
Accession Version	XM_002310683.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037291.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 9, 2018 this sequence version replaced XM_002310683.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG AGCAGCCAGC GGTGTTAAGT GGGAAGCGAA GAAGAGGAAC AGAAGCCACG 
GCCAGATCCG AAGCCCTTGA GCGACTCAAA GCGCTCCGTC GTGGTGGCAG GAGATCTGAA 
AATGGCGGCG GGTACGCCAT CAAAATGGAA GATCCAATCT ATGATTCCGT CCCCGAGGAC 
GAGTACAACA GTCTCGTCGC TCGCCGCCGA GAGGAAGCTC AGGGTTTCAT CGTTGACGAT 
GACGGCCTAG GTTACGGCGA CGAAGGTGAA GAAGAGGACT GGTCTCAGGC CGGATTACCT 
CCGTCTTCGG ATGAATCGGA CGGCGAGGGT TTGAATAAGA GTAGATCTAG AAAGAAGAAA 
AGTGAAAAGA AAGAGAAAGA TAAAGAGAAA GAAGTGAAGA AAGGTAATGC TTCGTTGACG 
GCTGCTGCGG CGGCGATTAT GGGGAAAACG AAGATATCGG CTATGTTTAC ATCAAATGTT 
TTTAAAAATA GGGATTCTGT TGGTAAGGTA AAGAGTTTTG ACTGTGACAA TATTGTTGAT 
GATGTTATCG CGGAGTTTGC TCCTGATGAA GCTGATAGAG AAAGGCGTAG AAGAGGACAG 
TTGATTGTGA AGAATGCTAC ACCAATTAAG AGTGAAAATC GATTTTTTGA TAACGGTAAT 
GATGTTAATT TTATGGTTAA AGCAGATTTG GATAGGGATT TTATTAATTT TAAGAGTGGT 
GTTGATGAGA AAAAAGAGGG AATTGTGGAA GTGAAAGAGA AGGGAGAGGA TAAATTGTCT 
AATCTGGTTA AAGAGGAGGT TGTAAGTGTT TTGGAAGTGA AAGCAGACGA GGCAGTTGTG 
AAGAAGGATG GTGGACGTAC ACTTAATGCG AAGATTAGTG ATGAGGAGAG GGATCCAGCT 
TTGAGTGCTA CAGCCGGGTG GAAAGAAGTG ATGAATGGTA AGAATGGGGG TGAGGCTGTT 
GGTGGCGCTG GGGTAGTGGA GGAGGTTAAA AGTGGAGCAA ATTGTGAGGA GCAATCTGAG 
TTTGAGCTGG ATGGTGACGG GTCATTACCC TTTTACATTC TTGATGCTCA TGAAGAGATT 
TTTGGTGCTA ACACGGGCAC GATCTATTTG TTTGGGAAGG TGAAAGCCGG AAATACATAT 
CATAGTTGCT GTGTGGTTGT AAAGAATATG CACAGATGTG TTTATGCAAT TCCAAATAGT 
TCTATATTTC GTACTGATGA GATGAGCATG CTAGAGAAAG AGGTTGAAGA GTCACGGATT 
TCTTCCACAG ATTTCCATAA AAAGCTGCAA GACATGGCAT ATGAACTAAA GAATGAAGTT 
GCAAGCCAAT TGTTAAGTCT CAATGTTTCA AGTTTCAGCA TGGCTCCGGT TAAGAGGAGA 
TATGCATTTG ACCGCTCTGA TATACCAGCT GGAGAGAATT ATGCACTCAA GATCAACTAT 
CCATTTAAGG AACCTCCGCT CCCAGCAGAT CTGAAAGGGG AAACTTTCTG TGCTTTGCTC 
GGAACTCATT GCAGTGCTTT AGAGCTTTTT CTCGTTAAAA GGAAGGTAAA GGGACCTTCG 
TGGCTTTCAG TTTCAAAATT TGCAACTTGC CCAGCTTCTC AAAAGGTGAG CTGGTGCAAG 
TTTGAGATCA TTGTCGAATC TCCAAAGGAC ATACAAGTTT CATCTTCATC AAATAGCAAA 
ATAGAGATTC CTCCAGTGGT TGTTGCTGCA ATAAATCTGA AAACTGTCAT AAATGAAAAG 
CAGAATGTGA ATGAAATAGT CTCTGCTTCT GTCATTTGCT GTCACAAGGC CAAGATTGAC 
ACTCCCATGT TGGCCTCCGA GTGGAAGAAA CCTGGCATGC TGAGCCATTT TACTGTTGTT 
CGCAAGCTTG ATGGAGGGAT ATTCCCAATG GGATTTTCCA AAGAGGTTAC AGACAGAAAC 
ACAATGGCTG GATCAAATGT CTTAGCTATT GAAAGCAGCG AAAGAGCTTT GTTAAATCGT 
CTGATGATTG CATTACACAA ATTAGACAGT GATTTTCTTG TTGGACATAA TATATCTGGT 
TTTGACCTGG ATGTTCTCCT CCATAGGACA CAGGCTTGCC GGGTGCCAAG TAGCACATGG 
TCCAAAATAG GACGTCTCAA GCGTTCTGTG ATGCCTAAAC TTACAAAAGG AAATGCCATT 
TTTGGCTCTG GAGCAAGTCC AGGGATCATG TCTTGCATTG CTGGCCGGCT CTTATGCGAT 
ACATATTTGG CTTCCCGTGA TTTATTAAAA GAGGTTAGTT ATTCCTTGAC GCAACTTGCT 
AAGACTCGGC TGAACAAAGA CCGCAAGGAG ATTGCACCAC ATGATATTCC CACAATGTTC 
CAGACGTCAA AGTCCCTTAT TGAACTAGTT GAATATGGTG AGACAGATGC ATGGTTATCT 
ATGGAACTCA TGTTTCATTT GAGCATTCTT CCTCTCACAC GCCAGCTGAC CAATATCAGT 
GGGAATCTCT GGGGGAAAAC TCTTCAAGGT GCGAGGGCAC AGAGAGTAGA GTATCTTCTA 
TTGCATGCAT TCCATGCAAA GAAATATATT GTCCCAGACA AGATGTCTTC TCGTGTAAAG 
GAAGCAAAAA TAACAAAGAG GAGAATTAAT AATGGCATTG AGGATAGAAA TGCTGATGAG 
TTGGACACTG ATGCTGCCAA TTTCGAAAAT GACAATCAAC AGAGTGATCA TGGGAAAAGA 
AAAAAAGGTC CTGCCTATGC AGGTGGATTG GTTTTGGAGC CTAAAAAAGG TTTATATGAC 
AAATATGTAT TGCTTCTGGA TTTCAATAGT CTTTACCCCT CAATTATTCA GGAATACAAT 
ATCTGCTTCA CAACAGTTGA AAGGTCTATG GATGGATTGG TTCCTCGTTT ACCTTCCAGT 
AAAACAACTG GAGTTCTGCC TGAGTTGCTG AAAAATTTAG TTGAGAGAAG AAGAATGGTT 
AAGTCATGGA TGAAGAATGC ATCTGGTCTC AAAGTCCAGC AGCTTGACAT TCAGCAACAA 
GCACTTAAGC TCACTGCAAA TAGTATGTAT GGATGCCTTG GGTTTTCCAA TTCAAGATTC 
TATGCAAAGC CACTTGCAGA ACTAATAACT CTACAAGGAA GGGAGATTTT ACAAAGTACT 
GTTGATCTTG TTCAGAACAA TTTGAACTTG GAGGTGATCT ATGGTGATAC AGATTCAATT 
ATGATTTATA GTGGACTAGA TGATATTCCA AAAGCAAAAG CTATTGCAGG GAAAGTCATC 
CAGGAGGTTA ACAAGAAGTA CAGGTGTCTA GAAATTGATC TTGATGGCTT GTACAAAAGA 
ATGCTTCTTC TAAAGAAGAA GAAATATGCA GCTGTTAAGG TGCAATTCAA GGATGGGACA 
CCATATGAGG TTATTGAACG TAAAGGTCTT GACATGGTTC GTCGTGACTG GAGCTTGCTA 
TCAAAGGAAT TGGGAGATTT CTGCCTGGCT CAAATTTTGT CTGGAGGGTC ATGCGAGGAC 
GTAATTGAAT CAATACATAA CTCCCTCATG AAGGTCCAGG AAGATATGAG GAGTGGACAA 
GTAGCCCTTG AGAAATATGT CATCACAAAG ACATTAACTA AACCACCTGA AGCCTATCCT 
GATGCCAAAA ATCAACCCCA TGCTCTGGTA GCACTAAGGT TGAAGCAAAG TGGTTATACA 
GCTGGTTGCT CTGCTGGTGA TACAGTACCG TATATAATTT GCTGTGAGCA GGGTGCGGGT 
GCTTCTGCAG TAAGTTTAAC AGGGATAGCC CAGCGTGCAA GACATCCTGA TGAATTGAAA 
TGTGATGATG GGAAATGGAT TATTGACATT GAGTACTACT TGTCACAGCA AATTCATCCT 
GTGGTATCAC GTTTATGTGC TTCAATCCAG GGTACAAGCC CAGAGCGTTT GGCTGATTGC 
TTGGGGCTTG ACTCATCCAA GTTCCGTAGT AAATCAAGTG AATCTGTCCC TTCTAGCTCA 
CTCCTGTTTG CTGCTGATGA TGAGGAAAGA TATCAGAGTT GTGAGCCGCT AATCTTGTCA 
TGTCCCAGCT GCTCTGGTAC TTTTCACTGT CCTCCTGTCT TCATTTCCAT TTGTACGTCA 
ATTCTTGAGA AATCAAAAAA CCCACAAATC GAAGAATCTA TATCCAATTT CTGGCATAGA 
TTGTGTTGCC CTAAATGCCC AGAGGAAGGT GACTTGGGCA GAATATCTCC TGCAACGATG 
GCCAATCAGG TCAAAAGGCA AGCAGAGGGG TTTGTTTCCA CCTACTATAA AGGCGTCATG 
ATGTGTGATG ATGAAACTTG TAAGCATACC ACTAGGAGTC TCAACCTGAG GCTGGTTGGT 
GATTCCGAGA GAGGAACAGT TTGTCCAAGC TATCCACGCT GCAATGGGCG TCTAGTGAGA 
AAGTATACGG AAGCAGATTT GTACAAGCAA CTGTCGTATT TCTGTCACTT GCTCGAGACT 
GCTCGCTGCA TTGAAAAGTT GCAGGCGGAC GCAGGCACTA GAATACAGGT AGAGAAGGAG 
TTGATGAAAA TCCGGCCAAT GGTTGATATG GCACTTTCAA CAGTTAAGAA GATTAGGGAC 
AGATGTGCTT ATGGGTGGGT GCAGTTGAAT GGCCTTGCAG TCACAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002310719.3
CDS	118..4728
Translation	MADEQPAVLSGKRRRGTEATARSEALERLKALRRGGRRSENGGGYAIKMEDPIYDSVPEDEYNSLVARRREEAQGFIVDDDGLGYGDEGEEEDWSQAGLPPSSDESDGEGLNKSRSRKKKSEKKEKDKEKEVKKGNASLTAAAAAIMGKTKISAMFTSNVFKNRDSVGKVKSFDCDNIVDDVIAEFAPDEADRERRRRGQLIVKNATPIKSENRFFDNGNDVNFMVKADLDRDFINFKSGVDEKKEGIVEVKEKGEDKLSNLVKEEVVSVLEVKADEAVVKKDGGRTLNAKISDEERDPALSATAGWKEVMNGKNGGEAVGGAGVVEEVKSGANCEEQSEFELDGDGSLPFYILDAHEEIFGANTGTIYLFGKVKAGNTYHSCCVVVKNMHRCVYAIPNSSIFRTDEMSMLEKEVEESRISSTDFHKKLQDMAYELKNEVASQLLSLNVSSFSMAPVKRRYAFDRSDIPAGENYALKINYPFKEPPLPADLKGETFCALLGTHCSALELFLVKRKVKGPSWLSVSKFATCPASQKVSWCKFEIIVESPKDIQVSSSSNSKIEIPPVVVAAINLKTVINEKQNVNEIVSASVICCHKAKIDTPMLASEWKKPGMLSHFTVVRKLDGGIFPMGFSKEVTDRNTMAGSNVLAIESSERALLNRLMIALHKLDSDFLVGHNISGFDLDVLLHRTQACRVPSSTWSKIGRLKRSVMPKLTKGNAIFGSGASPGIMSCIAGRLLCDTYLASRDLLKEVSYSLTQLAKTRLNKDRKEIAPHDIPTMFQTSKSLIELVEYGETDAWLSMELMFHLSILPLTRQLTNISGNLWGKTLQGARAQRVEYLLLHAFHAKKYIVPDKMSSRVKEAKITKRRINNGIEDRNADELDTDAANFENDNQQSDHGKRKKGPAYAGGLVLEPKKGLYDKYVLLLDFNSLYPSIIQEYNICFTTVERSMDGLVPRLPSSKTTGVLPELLKNLVERRRMVKSWMKNASGLKVQQLDIQQQALKLTANSMYGCLGFSNSRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDDIPKAKAIAGKVIQEVNKKYRCLEIDLDGLYKRMLLLKKKKYAAVKVQFKDGTPYEVIERKGLDMVRRDWSLLSKELGDFCLAQILSGGSCEDVIESIHNSLMKVQEDMRSGQVALEKYVITKTLTKPPEAYPDAKNQPHALVALRLKQSGYTAGCSAGDTVPYIICCEQGAGASAVSLTGIAQRARHPDELKCDDGKWIIDIEYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFRSKSSESVPSSSLLFAADDEERYQSCEPLILSCPSCSGTFHCPPVFISICTSILEKSKNPQIEESISNFWHRLCCPKCPEEGDLGRISPATMANQVKRQAEGFVSTYYKGVMMCDDETCKHTTRSLNLRLVGDSERGTVCPSYPRCNGRLVRKYTEADLYKQLSYFCHLLETARCIEKLQADAGTRIQVEKELMKIRPMVDMALSTVKKIRDRCAYGWVQLNGLAVTV

Target ORF information:

RefSeq Version	XM_002310683.3
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa DNA polymerase alpha catalytic subunit (LOC7483107), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002310683.3

ORF Insert Sequence:

ATGGCGGACG AGCAGCCAGC GGTGTTAAGT GGGAAGCGAA GAAGAGGAAC AGAAGCCACG 
GCCAGATCCG AAGCCCTTGA GCGACTCAAA GCGCTCCGTC GTGGTGGCAG GAGATCTGAA 
AATGGCGGCG GGTACGCCAT CAAAATGGAA GATCCAATCT ATGATTCCGT CCCCGAGGAC 
GAGTACAACA GTCTCGTCGC TCGCCGCCGA GAGGAAGCTC AGGGTTTCAT CGTTGACGAT 
GACGGCCTAG GTTACGGCGA CGAAGGTGAA GAAGAGGACT GGTCTCAGGC CGGATTACCT 
CCGTCTTCGG ATGAATCGGA CGGCGAGGGT TTGAATAAGA GTAGATCTAG AAAGAAGAAA 
AGTGAAAAGA AAGAGAAAGA TAAAGAGAAA GAAGTGAAGA AAGGTAATGC TTCGTTGACG 
GCTGCTGCGG CGGCGATTAT GGGGAAAACG AAGATATCGG CTATGTTTAC ATCAAATGTT 
TTTAAAAATA GGGATTCTGT TGGTAAGGTA AAGAGTTTTG ACTGTGACAA TATTGTTGAT 
GATGTTATCG CGGAGTTTGC TCCTGATGAA GCTGATAGAG AAAGGCGTAG AAGAGGACAG 
TTGATTGTGA AGAATGCTAC ACCAATTAAG AGTGAAAATC GATTTTTTGA TAACGGTAAT 
GATGTTAATT TTATGGTTAA AGCAGATTTG GATAGGGATT TTATTAATTT TAAGAGTGGT 
GTTGATGAGA AAAAAGAGGG AATTGTGGAA GTGAAAGAGA AGGGAGAGGA TAAATTGTCT 
AATCTGGTTA AAGAGGAGGT TGTAAGTGTT TTGGAAGTGA AAGCAGACGA GGCAGTTGTG 
AAGAAGGATG GTGGACGTAC ACTTAATGCG AAGATTAGTG ATGAGGAGAG GGATCCAGCT 
TTGAGTGCTA CAGCCGGGTG GAAAGAAGTG ATGAATGGTA AGAATGGGGG TGAGGCTGTT 
GGTGGCGCTG GGGTAGTGGA GGAGGTTAAA AGTGGAGCAA ATTGTGAGGA GCAATCTGAG 
TTTGAGCTGG ATGGTGACGG GTCATTACCC TTTTACATTC TTGATGCTCA TGAAGAGATT 
TTTGGTGCTA ACACGGGCAC GATCTATTTG TTTGGGAAGG TGAAAGCCGG AAATACATAT 
CATAGTTGCT GTGTGGTTGT AAAGAATATG CACAGATGTG TTTATGCAAT TCCAAATAGT 
TCTATATTTC GTACTGATGA GATGAGCATG CTAGAGAAAG AGGTTGAAGA GTCACGGATT 
TCTTCCACAG ATTTCCATAA AAAGCTGCAA GACATGGCAT ATGAACTAAA GAATGAAGTT 
GCAAGCCAAT TGTTAAGTCT CAATGTTTCA AGTTTCAGCA TGGCTCCGGT TAAGAGGAGA 
TATGCATTTG ACCGCTCTGA TATACCAGCT GGAGAGAATT ATGCACTCAA GATCAACTAT 
CCATTTAAGG AACCTCCGCT CCCAGCAGAT CTGAAAGGGG AAACTTTCTG TGCTTTGCTC 
GGAACTCATT GCAGTGCTTT AGAGCTTTTT CTCGTTAAAA GGAAGGTAAA GGGACCTTCG 
TGGCTTTCAG TTTCAAAATT TGCAACTTGC CCAGCTTCTC AAAAGGTGAG CTGGTGCAAG 
TTTGAGATCA TTGTCGAATC TCCAAAGGAC ATACAAGTTT CATCTTCATC AAATAGCAAA 
ATAGAGATTC CTCCAGTGGT TGTTGCTGCA ATAAATCTGA AAACTGTCAT AAATGAAAAG 
CAGAATGTGA ATGAAATAGT CTCTGCTTCT GTCATTTGCT GTCACAAGGC CAAGATTGAC 
ACTCCCATGT TGGCCTCCGA GTGGAAGAAA CCTGGCATGC TGAGCCATTT TACTGTTGTT 
CGCAAGCTTG ATGGAGGGAT ATTCCCAATG GGATTTTCCA AAGAGGTTAC AGACAGAAAC 
ACAATGGCTG GATCAAATGT CTTAGCTATT GAAAGCAGCG AAAGAGCTTT GTTAAATCGT 
CTGATGATTG CATTACACAA ATTAGACAGT GATTTTCTTG TTGGACATAA TATATCTGGT 
TTTGACCTGG ATGTTCTCCT CCATAGGACA CAGGCTTGCC GGGTGCCAAG TAGCACATGG 
TCCAAAATAG GACGTCTCAA GCGTTCTGTG ATGCCTAAAC TTACAAAAGG AAATGCCATT 
TTTGGCTCTG GAGCAAGTCC AGGGATCATG TCTTGCATTG CTGGCCGGCT CTTATGCGAT 
ACATATTTGG CTTCCCGTGA TTTATTAAAA GAGGTTAGTT ATTCCTTGAC GCAACTTGCT 
AAGACTCGGC TGAACAAAGA CCGCAAGGAG ATTGCACCAC ATGATATTCC CACAATGTTC 
CAGACGTCAA AGTCCCTTAT TGAACTAGTT GAATATGGTG AGACAGATGC ATGGTTATCT 
ATGGAACTCA TGTTTCATTT GAGCATTCTT CCTCTCACAC GCCAGCTGAC CAATATCAGT 
GGGAATCTCT GGGGGAAAAC TCTTCAAGGT GCGAGGGCAC AGAGAGTAGA GTATCTTCTA 
TTGCATGCAT TCCATGCAAA GAAATATATT GTCCCAGACA AGATGTCTTC TCGTGTAAAG 
GAAGCAAAAA TAACAAAGAG GAGAATTAAT AATGGCATTG AGGATAGAAA TGCTGATGAG 
TTGGACACTG ATGCTGCCAA TTTCGAAAAT GACAATCAAC AGAGTGATCA TGGGAAAAGA 
AAAAAAGGTC CTGCCTATGC AGGTGGATTG GTTTTGGAGC CTAAAAAAGG TTTATATGAC 
AAATATGTAT TGCTTCTGGA TTTCAATAGT CTTTACCCCT CAATTATTCA GGAATACAAT 
ATCTGCTTCA CAACAGTTGA AAGGTCTATG GATGGATTGG TTCCTCGTTT ACCTTCCAGT 
AAAACAACTG GAGTTCTGCC TGAGTTGCTG AAAAATTTAG TTGAGAGAAG AAGAATGGTT 
AAGTCATGGA TGAAGAATGC ATCTGGTCTC AAAGTCCAGC AGCTTGACAT TCAGCAACAA 
GCACTTAAGC TCACTGCAAA TAGTATGTAT GGATGCCTTG GGTTTTCCAA TTCAAGATTC 
TATGCAAAGC CACTTGCAGA ACTAATAACT CTACAAGGAA GGGAGATTTT ACAAAGTACT 
GTTGATCTTG TTCAGAACAA TTTGAACTTG GAGGTGATCT ATGGTGATAC AGATTCAATT 
ATGATTTATA GTGGACTAGA TGATATTCCA AAAGCAAAAG CTATTGCAGG GAAAGTCATC 
CAGGAGGTTA ACAAGAAGTA CAGGTGTCTA GAAATTGATC TTGATGGCTT GTACAAAAGA 
ATGCTTCTTC TAAAGAAGAA GAAATATGCA GCTGTTAAGG TGCAATTCAA GGATGGGACA 
CCATATGAGG TTATTGAACG TAAAGGTCTT GACATGGTTC GTCGTGACTG GAGCTTGCTA 
TCAAAGGAAT TGGGAGATTT CTGCCTGGCT CAAATTTTGT CTGGAGGGTC ATGCGAGGAC 
GTAATTGAAT CAATACATAA CTCCCTCATG AAGGTCCAGG AAGATATGAG GAGTGGACAA 
GTAGCCCTTG AGAAATATGT CATCACAAAG ACATTAACTA AACCACCTGA AGCCTATCCT 
GATGCCAAAA ATCAACCCCA TGCTCTGGTA GCACTAAGGT TGAAGCAAAG TGGTTATACA 
GCTGGTTGCT CTGCTGGTGA TACAGTACCG TATATAATTT GCTGTGAGCA GGGTGCGGGT 
GCTTCTGCAG TAAGTTTAAC AGGGATAGCC CAGCGTGCAA GACATCCTGA TGAATTGAAA 
TGTGATGATG GGAAATGGAT TATTGACATT GAGTACTACT TGTCACAGCA AATTCATCCT 
GTGGTATCAC GTTTATGTGC TTCAATCCAG GGTACAAGCC CAGAGCGTTT GGCTGATTGC 
TTGGGGCTTG ACTCATCCAA GTTCCGTAGT AAATCAAGTG AATCTGTCCC TTCTAGCTCA 
CTCCTGTTTG CTGCTGATGA TGAGGAAAGA TATCAGAGTT GTGAGCCGCT AATCTTGTCA 
TGTCCCAGCT GCTCTGGTAC TTTTCACTGT CCTCCTGTCT TCATTTCCAT TTGTACGTCA 
ATTCTTGAGA AATCAAAAAA CCCACAAATC GAAGAATCTA TATCCAATTT CTGGCATAGA 
TTGTGTTGCC CTAAATGCCC AGAGGAAGGT GACTTGGGCA GAATATCTCC TGCAACGATG 
GCCAATCAGG TCAAAAGGCA AGCAGAGGGG TTTGTTTCCA CCTACTATAA AGGCGTCATG 
ATGTGTGATG ATGAAACTTG TAAGCATACC ACTAGGAGTC TCAACCTGAG GCTGGTTGGT 
GATTCCGAGA GAGGAACAGT TTGTCCAAGC TATCCACGCT GCAATGGGCG TCTAGTGAGA 
AAGTATACGG AAGCAGATTT GTACAAGCAA CTGTCGTATT TCTGTCACTT GCTCGAGACT 
GCTCGCTGCA TTGAAAAGTT GCAGGCGGAC GCAGGCACTA GAATACAGGT AGAGAAGGAG 
TTGATGAAAA TCCGGCCAAT GGTTGATATG GCACTTTCAA CAGTTAAGAA GATTAGGGAC 
AGATGTGCTT ATGGGTGGGT GCAGTTGAAT GGCCTTGCAG TCACAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0007s10880g ( XM_024605513.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024461281.1 Populus trichocarpa DNA polymerase alpha catalytic subunit (LOC7483107), transcript variant X3, mRNA.

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CloneID	OPa433538
Clone ID Related Accession (Same CDS sequence)	XM_024605513.1
Accession Version	XM_024605513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	DNA polymerase alpha catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037291.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG AGCAGCCAGC GGTGTTAAGT GGGAAGCGAA GAAGAGGAAC AGAAGCCACG 
GCCAGATCCG AAGCCCTTGA GCGACTCAAA GCGCTCCGTC GTGGTGGCAG GAGATCTGAA 
AATGGCGGCG GGTACGCCAT CAAAATGGAA GATCCAATCT ATGATTCCGT CCCCGAGGAC 
GAGTACAACA GTCTCGTCGC TCGCCGCCGA GAGGAAGCTC AGGGTTTCAT CGTTGACGAT 
GACGGCCTAG GTTACGGCGA CGAAGGTGAA GAAGAGGACT GGTCTCAGGC CGGATTACCT 
CCGTCTTCGG ATGAATCGGA CGGCGAGGGT TTGAATAAGA GTAGATCTAG AAAGAAGAAA 
AGTGAAAAGA AAGAGAAAGA TAAAGAGAAA GAAGTGAAGA AAGGTAATGC TTCGTTGACG 
GCTGCTGCGG CGGCGATTAT GGGGAAAACG AAGATATCGG CTATGTTTAC ATCAAATGTT 
TTTAAAAATA GGGATTCTGT TGGTAAGGTA AAGAGTTTTG ACTGTGACAA TATTGTTGAT 
GATGTTATCG CGGAGTTTGC TCCTGATGAA GCTGATAGAG AAAGGCGTAG AAGAGGACAG 
TTGATTGTGA AGAATGCTAC ACCAATTAAG AGTGAAAATC GATTTTTTGA TAACGGTAAT 
GATGTTAATT TTATGGTTAA AGCAGATTTG GATAGGGATT TTATTAATTT TAAGAGTGGT 
GTTGATGAGA AAAAAGAGGG AATTGTGGAA GTGAAAGAGA AGGGAGAGGA TAAATTGTCT 
AATCTGGTTA AAGAGGAGGT TGTAAGTGTT TTGGAAGTGA AAGCAGACGA GGCAGTTGTG 
AAGAAGGATG GTGGACGTAC ACTTAATGCG AAGATTAGTG ATGAGGAGAG GGATCCAGCT 
TTGAGTGCTA CAGCCGGGTG GAAAGAAGTG ATGAATGGTA AGAATGGGGG TGAGGCTGTT 
GGTGGCGCTG GGGTAGTGGA GGAGGTTAAA AGTGGAGCAA ATTGTGAGGA GCAATCTGAG 
TTTGAGCTGG ATGGTGACGG GTCATTACCC TTTTACATTC TTGATGCTCA TGAAGAGATT 
TTTGGTGCTA ACACGGGCAC GATCTATTTG TTTGGGAAGG TGAAAGCCGG AAATACATAT 
CATAGTTGCT GTGTGGTTGT AAAGAATATG CACAGATGTG TTTATGCAAT TCCAAATAGT 
TCTATATTTC GTACTGATGA GATGAGCATG CTAGAGAAAG AGGTTGAAGA GTCACGGATT 
TCTTCCACAG ATTTCCATAA AAAGCTGCAA GACATGGCAT ATGAACTAAA GAATGAAGTT 
GCAAGCCAAT TGTTAAGTCT CAATGTTTCA AGTTTCAGCA TGGCTCCGGT TAAGAGGAGA 
TATGCATTTG ACCGCTCTGA TATACCAGCT GGAGAGAATT ATGCACTCAA GATCAACTAT 
CCATTTAAGG AACCTCCGCT CCCAGCAGAT CTGAAAGGGG AAACTTTCTG TGCTTTGCTC 
GGAACTCATT GCAGTGCTTT AGAGCTTTTT CTCGTTAAAA GGAAGGTAAA GGGACCTTCG 
TGGCTTTCAG TTTCAAAATT TGCAACTTGC CCAGCTTCTC AAAAGGTGAG CTGGTGCAAG 
TTTGAGATCA TTGTCGAATC TCCAAAGGAC ATACAAGTTT CATCTTCATC AAATAGCAAA 
ATAGAGATTC CTCCAGTGGT TGTTGCTGCA ATAAATCTGA AAACTGTCAT AAATGAAAAG 
CAGAATGTGA ATGAAATAGT CTCTGCTTCT GTCATTTGCT GTCACAAGGC CAAGATTGAC 
ACTCCCATGT TGGCCTCCGA GTGGAAGAAA CCTGGCATGC TGAGCCATTT TACTGTTGTT 
CGCAAGCTTG ATGGAGGGAT ATTCCCAATG GGATTTTCCA AAGAGGTTAC AGACAGAAAC 
ACAATGGCTG GATCAAATGT CTTAGCTATT GAAAGCAGCG AAAGAGCTTT GTTAAATCGT 
CTGATGATTG CATTACACAA ATTAGACAGT GATTTTCTTG TTGGACATAA TATATCTGGT 
TTTGACCTGG ATGTTCTCCT CCATAGGACA CAGGCTTGCC GGGTGCCAAG TAGCACATGG 
TCCAAAATAG GACGTCTCAA GCGTTCTGTG ATGCCTAAAC TTACAAAAGG AAATGCCATT 
TTTGGCTCTG GAGCAAGTCC AGGGATCATG TCTTGCATTG CTGGCCGGCT CTTATGCGAT 
ACATATTTGG CTTCCCGTGA TTTATTAAAA GAGGTTAGTT ATTCCTTGAC GCAACTTGCT 
AAGACTCGGC TGAACAAAGA CCGCAAGGAG ATTGCACCAC ATGATATTCC CACAATGTTC 
CAGACGTCAA AGTCCCTTAT TGAACTAGTT GAATATGGTG AGACAGATGC ATGGTTATCT 
ATGGAACTCA TGTTTCATTT GAGCATTCTT CCTCTCACAC GCCAGCTGAC CAATATCAGT 
GGGAATCTCT GGGGGAAAAC TCTTCAAGGT GCGAGGGCAC AGAGAGTAGA GTATCTTCTA 
TTGCATGCAT TCCATGCAAA GAAATATATT GTCCCAGACA AGATGTCTTC TCGTGTAAAG 
GAAGCAAAAA TAACAAAGAG GAGAATTAAT AATGGCATTG AGGATAGAAA TGCTGATGAG 
TTGGACACTG ATGCTGCCAA TTTCGAAAAT GACAATCAAC AGAGTGATCA TGGGAAAAGA 
AAAAAAGGTC CTGCCTATGC AGGTGGATTG GTTTTGGAGC CTAAAAAAGG TTTATATGAC 
AAATATGTAT TGCTTCTGGA TTTCAATAGT CTTTACCCCT CAATTATTCA GGAATACAAT 
ATCTGCTTCA CAACAGTTGA AAGGTCTATG GATGGATTGG TTCCTCGTTT ACCTTCCAGT 
AAAACAACTG GAGTTCTGCC TGAGTTGCTG AAAAATTTAG TTGAGAGAAG AAGAATGGTT 
AAGTCATGGA TGAAGAATGC ATCTGGTCTC AAAGTCCAGC AGCTTGACAT TCAGCAACAA 
GCACTTAAGC TCACTGCAAA TAGTATGTAT GGATGCCTTG GGTTTTCCAA TTCAAGATTC 
TATGCAAAGC CACTTGCAGA ACTAATAACT CTACAAGGAA GGGAGATTTT ACAAAGTACT 
GTTGATCTTG TTCAGAACAA TTTGAACTTG GAGGTGATCT ATGGTGATAC AGATTCAATT 
ATGATTTATA GTGGACTAGA TGATATTCCA AAAGCAAAAG CTATTGCAGG GAAAGTCATC 
CAGGAGGTTA ACAAGAAGTA CAGGTGTCTA GAAATTGATC TTGATGGCTT GTACAAAAGA 
ATGCTTCTTC TAAAGAAGAA GAAATATGCA GCTGTTAAGG TGCAATTCAA GGATGGGACA 
CCATATGAGG TTATTGAACG TAAAGGTCTT GACATGGTTC GTCGTGACTG GAGCTTGCTA 
TCAAAGGAAT TGGGAGATTT CTGCCTGGCT CAAATTTTGT CTGGAGGGTC ATGCGAGGAC 
GTAATTGAAT CAATACATAA CTCCCTCATG AAGGTCCAGG AAGATATGAG GAGTGGACAA 
GTAGCCCTTG AGAAATATGT CATCACAAAG ACATTAACTA AACCACCTGA AGCCTATCCT 
GATGCCAAAA ATCAACCCCA TGCTCTGGTA GCACTAAGGT TGAAGCAAAG TGGTTATACA 
GCTGGTTGCT CTGCTGGTGA TACAGTACCG TATATAATTT GCTGTGAGCA GGGTGCGGGT 
GCTTCTGCAG TAAGTTTAAC AGGGATAGCC CAGCGTGCAA GACATCCTGA TGAATTGAAA 
TGTGATGATG GGAAATGGAT TATTGACATT GAGTACTACT TGTCACAGCA AATTCATCCT 
GTGGTATCAC GTTTATGTGC TTCAATCCAG GGTACAAGCC CAGAGCGTTT GGCTGATTGC 
TTGGGGCTTG ACTCATCCAA GTTCCGTAGT AAATCAAGTG AATCTGTCCC TTCTAGCTCA 
CTCCTGTTTG CTGCTGATGA TGAGGAAAGA TATCAGAGTT GTGAGCCGCT AATCTTGTCA 
TGTCCCAGCT GCTCTGGTAC TTTTCACTGT CCTCCTGTCT TCATTTCCAT TTGTACGTCA 
ATTCTTGAGA AATCAAAAAA CCCACAAATC GAAGAATCTA TATCCAATTT CTGGCATAGA 
TTGTGTTGCC CTAAATGCCC AGAGGAAGGT GACTTGGGCA GAATATCTCC TGCAACGATG 
GCCAATCAGG TCAAAAGGCA AGCAGAGGGG TTTGTTTCCA CCTACTATAA AGGCGTCATG 
ATGTGTGATG ATGAAACTTG TAAGCATACC ACTAGGAGTC TCAACCTGAG GCTGGTTGGT 
GATTCCGAGA GAGGAACAGT TTGTCCAAGC TATCCACGCT GCAATGGGCG TCTAGTGAGA 
AAGTATACGG AAGCAGATTT GTACAAGCAA CTGTCGTATT TCTGTCACTT GCTCGAGACT 
GCTCGCTGCA TTGAAAAGGC GGACGCAGGC ACTAGAATAC AGGTAGAGAA GGAGTTGATG 
AAAATCCGGC CAATGGTTGA TATGGCACTT TCAACAGTTA AGAAGATTAG GGACAGATGT 
GCTTATGGGT GGGTGCAGTT GAATGGCCTT GCAGTCACAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024461281.1
CDS	118..4722
Translation	MADEQPAVLSGKRRRGTEATARSEALERLKALRRGGRRSENGGGYAIKMEDPIYDSVPEDEYNSLVARRREEAQGFIVDDDGLGYGDEGEEEDWSQAGLPPSSDESDGEGLNKSRSRKKKSEKKEKDKEKEVKKGNASLTAAAAAIMGKTKISAMFTSNVFKNRDSVGKVKSFDCDNIVDDVIAEFAPDEADRERRRRGQLIVKNATPIKSENRFFDNGNDVNFMVKADLDRDFINFKSGVDEKKEGIVEVKEKGEDKLSNLVKEEVVSVLEVKADEAVVKKDGGRTLNAKISDEERDPALSATAGWKEVMNGKNGGEAVGGAGVVEEVKSGANCEEQSEFELDGDGSLPFYILDAHEEIFGANTGTIYLFGKVKAGNTYHSCCVVVKNMHRCVYAIPNSSIFRTDEMSMLEKEVEESRISSTDFHKKLQDMAYELKNEVASQLLSLNVSSFSMAPVKRRYAFDRSDIPAGENYALKINYPFKEPPLPADLKGETFCALLGTHCSALELFLVKRKVKGPSWLSVSKFATCPASQKVSWCKFEIIVESPKDIQVSSSSNSKIEIPPVVVAAINLKTVINEKQNVNEIVSASVICCHKAKIDTPMLASEWKKPGMLSHFTVVRKLDGGIFPMGFSKEVTDRNTMAGSNVLAIESSERALLNRLMIALHKLDSDFLVGHNISGFDLDVLLHRTQACRVPSSTWSKIGRLKRSVMPKLTKGNAIFGSGASPGIMSCIAGRLLCDTYLASRDLLKEVSYSLTQLAKTRLNKDRKEIAPHDIPTMFQTSKSLIELVEYGETDAWLSMELMFHLSILPLTRQLTNISGNLWGKTLQGARAQRVEYLLLHAFHAKKYIVPDKMSSRVKEAKITKRRINNGIEDRNADELDTDAANFENDNQQSDHGKRKKGPAYAGGLVLEPKKGLYDKYVLLLDFNSLYPSIIQEYNICFTTVERSMDGLVPRLPSSKTTGVLPELLKNLVERRRMVKSWMKNASGLKVQQLDIQQQALKLTANSMYGCLGFSNSRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDDIPKAKAIAGKVIQEVNKKYRCLEIDLDGLYKRMLLLKKKKYAAVKVQFKDGTPYEVIERKGLDMVRRDWSLLSKELGDFCLAQILSGGSCEDVIESIHNSLMKVQEDMRSGQVALEKYVITKTLTKPPEAYPDAKNQPHALVALRLKQSGYTAGCSAGDTVPYIICCEQGAGASAVSLTGIAQRARHPDELKCDDGKWIIDIEYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFRSKSSESVPSSSLLFAADDEERYQSCEPLILSCPSCSGTFHCPPVFISICTSILEKSKNPQIEESISNFWHRLCCPKCPEEGDLGRISPATMANQVKRQAEGFVSTYYKGVMMCDDETCKHTTRSLNLRLVGDSERGTVCPSYPRCNGRLVRKYTEADLYKQLSYFCHLLETARCIEKADAGTRIQVEKELMKIRPMVDMALSTVKKIRDRCAYGWVQLNGLAVTV

Target ORF information:

RefSeq Version	XM_024605513.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa DNA polymerase alpha catalytic subunit (LOC7483107), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024605513.1

ORF Insert Sequence:

ATGGCGGACG AGCAGCCAGC GGTGTTAAGT GGGAAGCGAA GAAGAGGAAC AGAAGCCACG 
GCCAGATCCG AAGCCCTTGA GCGACTCAAA GCGCTCCGTC GTGGTGGCAG GAGATCTGAA 
AATGGCGGCG GGTACGCCAT CAAAATGGAA GATCCAATCT ATGATTCCGT CCCCGAGGAC 
GAGTACAACA GTCTCGTCGC TCGCCGCCGA GAGGAAGCTC AGGGTTTCAT CGTTGACGAT 
GACGGCCTAG GTTACGGCGA CGAAGGTGAA GAAGAGGACT GGTCTCAGGC CGGATTACCT 
CCGTCTTCGG ATGAATCGGA CGGCGAGGGT TTGAATAAGA GTAGATCTAG AAAGAAGAAA 
AGTGAAAAGA AAGAGAAAGA TAAAGAGAAA GAAGTGAAGA AAGGTAATGC TTCGTTGACG 
GCTGCTGCGG CGGCGATTAT GGGGAAAACG AAGATATCGG CTATGTTTAC ATCAAATGTT 
TTTAAAAATA GGGATTCTGT TGGTAAGGTA AAGAGTTTTG ACTGTGACAA TATTGTTGAT 
GATGTTATCG CGGAGTTTGC TCCTGATGAA GCTGATAGAG AAAGGCGTAG AAGAGGACAG 
TTGATTGTGA AGAATGCTAC ACCAATTAAG AGTGAAAATC GATTTTTTGA TAACGGTAAT 
GATGTTAATT TTATGGTTAA AGCAGATTTG GATAGGGATT TTATTAATTT TAAGAGTGGT 
GTTGATGAGA AAAAAGAGGG AATTGTGGAA GTGAAAGAGA AGGGAGAGGA TAAATTGTCT 
AATCTGGTTA AAGAGGAGGT TGTAAGTGTT TTGGAAGTGA AAGCAGACGA GGCAGTTGTG 
AAGAAGGATG GTGGACGTAC ACTTAATGCG AAGATTAGTG ATGAGGAGAG GGATCCAGCT 
TTGAGTGCTA CAGCCGGGTG GAAAGAAGTG ATGAATGGTA AGAATGGGGG TGAGGCTGTT 
GGTGGCGCTG GGGTAGTGGA GGAGGTTAAA AGTGGAGCAA ATTGTGAGGA GCAATCTGAG 
TTTGAGCTGG ATGGTGACGG GTCATTACCC TTTTACATTC TTGATGCTCA TGAAGAGATT 
TTTGGTGCTA ACACGGGCAC GATCTATTTG TTTGGGAAGG TGAAAGCCGG AAATACATAT 
CATAGTTGCT GTGTGGTTGT AAAGAATATG CACAGATGTG TTTATGCAAT TCCAAATAGT 
TCTATATTTC GTACTGATGA GATGAGCATG CTAGAGAAAG AGGTTGAAGA GTCACGGATT 
TCTTCCACAG ATTTCCATAA AAAGCTGCAA GACATGGCAT ATGAACTAAA GAATGAAGTT 
GCAAGCCAAT TGTTAAGTCT CAATGTTTCA AGTTTCAGCA TGGCTCCGGT TAAGAGGAGA 
TATGCATTTG ACCGCTCTGA TATACCAGCT GGAGAGAATT ATGCACTCAA GATCAACTAT 
CCATTTAAGG AACCTCCGCT CCCAGCAGAT CTGAAAGGGG AAACTTTCTG TGCTTTGCTC 
GGAACTCATT GCAGTGCTTT AGAGCTTTTT CTCGTTAAAA GGAAGGTAAA GGGACCTTCG 
TGGCTTTCAG TTTCAAAATT TGCAACTTGC CCAGCTTCTC AAAAGGTGAG CTGGTGCAAG 
TTTGAGATCA TTGTCGAATC TCCAAAGGAC ATACAAGTTT CATCTTCATC AAATAGCAAA 
ATAGAGATTC CTCCAGTGGT TGTTGCTGCA ATAAATCTGA AAACTGTCAT AAATGAAAAG 
CAGAATGTGA ATGAAATAGT CTCTGCTTCT GTCATTTGCT GTCACAAGGC CAAGATTGAC 
ACTCCCATGT TGGCCTCCGA GTGGAAGAAA CCTGGCATGC TGAGCCATTT TACTGTTGTT 
CGCAAGCTTG ATGGAGGGAT ATTCCCAATG GGATTTTCCA AAGAGGTTAC AGACAGAAAC 
ACAATGGCTG GATCAAATGT CTTAGCTATT GAAAGCAGCG AAAGAGCTTT GTTAAATCGT 
CTGATGATTG CATTACACAA ATTAGACAGT GATTTTCTTG TTGGACATAA TATATCTGGT 
TTTGACCTGG ATGTTCTCCT CCATAGGACA CAGGCTTGCC GGGTGCCAAG TAGCACATGG 
TCCAAAATAG GACGTCTCAA GCGTTCTGTG ATGCCTAAAC TTACAAAAGG AAATGCCATT 
TTTGGCTCTG GAGCAAGTCC AGGGATCATG TCTTGCATTG CTGGCCGGCT CTTATGCGAT 
ACATATTTGG CTTCCCGTGA TTTATTAAAA GAGGTTAGTT ATTCCTTGAC GCAACTTGCT 
AAGACTCGGC TGAACAAAGA CCGCAAGGAG ATTGCACCAC ATGATATTCC CACAATGTTC 
CAGACGTCAA AGTCCCTTAT TGAACTAGTT GAATATGGTG AGACAGATGC ATGGTTATCT 
ATGGAACTCA TGTTTCATTT GAGCATTCTT CCTCTCACAC GCCAGCTGAC CAATATCAGT 
GGGAATCTCT GGGGGAAAAC TCTTCAAGGT GCGAGGGCAC AGAGAGTAGA GTATCTTCTA 
TTGCATGCAT TCCATGCAAA GAAATATATT GTCCCAGACA AGATGTCTTC TCGTGTAAAG 
GAAGCAAAAA TAACAAAGAG GAGAATTAAT AATGGCATTG AGGATAGAAA TGCTGATGAG 
TTGGACACTG ATGCTGCCAA TTTCGAAAAT GACAATCAAC AGAGTGATCA TGGGAAAAGA 
AAAAAAGGTC CTGCCTATGC AGGTGGATTG GTTTTGGAGC CTAAAAAAGG TTTATATGAC 
AAATATGTAT TGCTTCTGGA TTTCAATAGT CTTTACCCCT CAATTATTCA GGAATACAAT 
ATCTGCTTCA CAACAGTTGA AAGGTCTATG GATGGATTGG TTCCTCGTTT ACCTTCCAGT 
AAAACAACTG GAGTTCTGCC TGAGTTGCTG AAAAATTTAG TTGAGAGAAG AAGAATGGTT 
AAGTCATGGA TGAAGAATGC ATCTGGTCTC AAAGTCCAGC AGCTTGACAT TCAGCAACAA 
GCACTTAAGC TCACTGCAAA TAGTATGTAT GGATGCCTTG GGTTTTCCAA TTCAAGATTC 
TATGCAAAGC CACTTGCAGA ACTAATAACT CTACAAGGAA GGGAGATTTT ACAAAGTACT 
GTTGATCTTG TTCAGAACAA TTTGAACTTG GAGGTGATCT ATGGTGATAC AGATTCAATT 
ATGATTTATA GTGGACTAGA TGATATTCCA AAAGCAAAAG CTATTGCAGG GAAAGTCATC 
CAGGAGGTTA ACAAGAAGTA CAGGTGTCTA GAAATTGATC TTGATGGCTT GTACAAAAGA 
ATGCTTCTTC TAAAGAAGAA GAAATATGCA GCTGTTAAGG TGCAATTCAA GGATGGGACA 
CCATATGAGG TTATTGAACG TAAAGGTCTT GACATGGTTC GTCGTGACTG GAGCTTGCTA 
TCAAAGGAAT TGGGAGATTT CTGCCTGGCT CAAATTTTGT CTGGAGGGTC ATGCGAGGAC 
GTAATTGAAT CAATACATAA CTCCCTCATG AAGGTCCAGG AAGATATGAG GAGTGGACAA 
GTAGCCCTTG AGAAATATGT CATCACAAAG ACATTAACTA AACCACCTGA AGCCTATCCT 
GATGCCAAAA ATCAACCCCA TGCTCTGGTA GCACTAAGGT TGAAGCAAAG TGGTTATACA 
GCTGGTTGCT CTGCTGGTGA TACAGTACCG TATATAATTT GCTGTGAGCA GGGTGCGGGT 
GCTTCTGCAG TAAGTTTAAC AGGGATAGCC CAGCGTGCAA GACATCCTGA TGAATTGAAA 
TGTGATGATG GGAAATGGAT TATTGACATT GAGTACTACT TGTCACAGCA AATTCATCCT 
GTGGTATCAC GTTTATGTGC TTCAATCCAG GGTACAAGCC CAGAGCGTTT GGCTGATTGC 
TTGGGGCTTG ACTCATCCAA GTTCCGTAGT AAATCAAGTG AATCTGTCCC TTCTAGCTCA 
CTCCTGTTTG CTGCTGATGA TGAGGAAAGA TATCAGAGTT GTGAGCCGCT AATCTTGTCA 
TGTCCCAGCT GCTCTGGTAC TTTTCACTGT CCTCCTGTCT TCATTTCCAT TTGTACGTCA 
ATTCTTGAGA AATCAAAAAA CCCACAAATC GAAGAATCTA TATCCAATTT CTGGCATAGA 
TTGTGTTGCC CTAAATGCCC AGAGGAAGGT GACTTGGGCA GAATATCTCC TGCAACGATG 
GCCAATCAGG TCAAAAGGCA AGCAGAGGGG TTTGTTTCCA CCTACTATAA AGGCGTCATG 
ATGTGTGATG ATGAAACTTG TAAGCATACC ACTAGGAGTC TCAACCTGAG GCTGGTTGGT 
GATTCCGAGA GAGGAACAGT TTGTCCAAGC TATCCACGCT GCAATGGGCG TCTAGTGAGA 
AAGTATACGG AAGCAGATTT GTACAAGCAA CTGTCGTATT TCTGTCACTT GCTCGAGACT 
GCTCGCTGCA TTGAAAAGGC GGACGCAGGC ACTAGAATAC AGGTAGAGAA GGAGTTGATG 
AAAATCCGGC CAATGGTTGA TATGGCACTT TCAACAGTTA AGAAGATTAG GGACAGATGT 
GCTTATGGGT GGGTGCAGTT GAATGGCCTT GCAGTCACAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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POPTR_0007s10880g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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