POPTR_0004s04320g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

Gene
Clones
gRNAs

Summary

Gene Symbol	POPTR_0004s04320g
Entrez Gene ID	7477407
Full Name	hypothetical protein
Synonyms	POPTRDRAFT_198024
Gene Type	protein-coding
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024599156.1	XP_024454924.1	DNA-directed RNA polymerase II subunit RPB2
XM_002305100.2	XP_002305136.2	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POPTR_0004s04320g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POPTR_0004s04320g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa10000	XM_002305100.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0004s04320g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa427900	XM_024599156.1 Latest version!	Populus trichocarpa DNA-directed RNA polymerase II subunit RPB2 (LOC7477407), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POPTR_0004s04320g ( XM_002305100.2 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002305136.2 Populus trichocarpa hypothetical protein (POPTR_0004s04320g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa10000
Clone ID Related Accession (Same CDS sequence)	XM_002305100.2
Accession Version	XM_002305100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008470). On Dec 16, 2013 this sequence version replaced XM_002305100.1.

ATGGAAGACC TTGACGAGTA CGATCAACAG CCGATGAATG AAGATATGGA ACCAGAAGAA 
GAAGACGCTG ATGAGGAGAT CACGCAGGAA GATGCCTGGG CCGTCATTTC TGCTTATTTT 
GAAGAGAAAG GCTTGGTGAG ACAACAACTC GACTCCTTCG ATGAGTTTAT TCAAAATACG 
ATGCAGGAAA TCGTTGACGA GTCTGCTGAT ATTGAGATTC GGCCTGAAAG CCAGCACAAT 
CCGGGTCACC AGTCTGATTT TGCTGAGACT ATCTACAAGA TCAGTTTTGG CCAGATATAC 
TTGAGTAAGC CCATGATGAC AGAGTCTGAT GGAGAAACTG CAACTTTGTT TCCCAAAGCT 
GCAAGGTTGA GGAACCTGAC ATACTCGGCT CCTTTGTATG TGGATGTTAC AAAGAGGGTT 
ATAAAGAAAG GTCATGATGG TGAAGAAGTC ACTGAAACTC AAGACTTCAC CAAAGTTTTC 
ATTGGAAAGG CGAGTATAAT GAATTATTTA TTTGTCCCTA TTATGCTTCG ATCAAGTTAT 
TGCACATTAT ACCAGAATTC AGAAAAGGAT TTGACAGAGC TTGGCGAGTG TCCGTATGAT 
CAGGGTGGGT ATTTCATTAT CAATGGTAGT GAAAAGGTTC TAATTGCTCA GGAGAAGATG 
AGCACCAATC ATGTGTATGT GTTCAAGAAG AGGCAGCCAA ACAAGTATGC ATATGTAGGA 
GAAGTTCGGT CTATGGCAGA GTCTCAGAAC CGGCCACCCA GTACCATGTT TGTGCGGATG 
CTTTCTCGGA CTAGTGCTAA AGGGGGCTCT TCAGGGCAAT ACATCCGTGC TACTCTTCCA 
TACATTCGAA CTGAAATTCC GATTATCATT GTTTTTCGTG CTTTGGGTTT TGTTGCTGAC 
AAAGACATTT TGGAACACAT ATGTTATGAT TTCGCTGATA CTCAAATGAT GGAGTTGCTT 
CGGCCATCTC TGGAAGAGGC ATTTGTTATT CAAAATCAGC AGGTTGCTCT TGACTACATT 
GGAAAGCGTG GGGCAACTGT TGGTGTAACT AGGGAAAAAA GGATTAAGTA TGCCAAAGAG 
ATTCTTCAAA AAGAAATGCT TCCTCATGTT GGTGTTGGAG AATACTGCGA GACCAAAAAA 
GCTTATTACT TCGGGTATAT CATTCACCGG CTTTTACTTT GTGCACTTGG CCGGAGGGCA 
GAAGATGATA GGGATCATTA TGGAAACAAG CGGCTTGACC TTGCTGGTCC TTTACTTGGG 
GGCTTGTTTA GAATGTTATT CAGAAAGTTG ACCAGGGATG TCAGGTCTTA TGTGCAAAAG 
TGTGTTGATA ATGGGAAGGA TGTTAACCTA CAATTTGCCA TTAAAGCAAA AACCATTACC 
AGTGGTCTTA AATACTCACT TGCCACTGGG AACTGGGGAC AAGCAAATGC AGCTGGAACA 
AGAGCTGGAG TGTCACAGGT GTTAAATAGA TTGACATATG CCTCTACACT GTCACATTTA 
AGAAGATTAA ATTCTCCAAT TGGGCGTGAA GGGAAATTGG CAAAACCAAG GCAGCTACAT 
AATTCACAAT GGGGAATGAT GTGTCCTGCT GAAACACCTG AAGGACAAGC TTGTGGATTA 
GTGAAGAATC TCGCATTGAT GGTATACATT ACAGTTGGAT CAGCTGCATA TCCCATCCTG 
GAGTTTTTAG AAGAATGGGG TACCGAGAAT TTTGAGGAAA TATCCCCTGC AGTTATTCCC 
CAAGCAACAA AAATTTTTGT CAATGGTGGC TGGGTGGGTA TCCATCGTGA TCCTGATATG 
TTGGTGAAGA CATTGAGACG GCTGCGGAGA CGGGTTGATG TCAATACTGA AGTTGGGGTT 
GTTAGAGATA TTCGCTTGAA AGAGTTGCGG ATTTACACTG ACTATGGCCG GTGCAGTCGT 
CCTCTATTCA TTGTGGAGAA ACAAAAGCTT CTTATAAGGA AGAAGGACAT CCATGCACTA 
CAACAGAGGG TGAGTCATTT AATTTATAAT GATCATCTTT TCAAATATGA GGGTGGCTGG 
CATGATCTTG TGTCAAAGGG ATTCATTGAA TATATTGACA CTGAGGAAGA AGAGACCACT 
ATGATATCCA TGACCATACA TGATCTTGTA CAAGCGAGGA TTAATCCAGA GGAAGCATAT 
TCTGATACTT ACACCCATTG TGAGATCCAC CCATCATTGA TTTTAGGTGT TTGTGCTTCT 
ATCATTCCTT TTCCTGACCA TAACCAGTCC CCACGTAACA CTTACCAATC TGCTATGGGA 
AAGCAAGCCA TGGGAATTTA TGTCACCAAT TACCAATTTC GAATGGATAC GCTGGCTTAT 
GTTCTCTATT ATCCCCAAAA GCCACTTGTT ACTACAAGAG CCATGGAGCA TCTTCATTTT 
CGGCAACTTC CTGCTGGCAT TAATGCTATT GTTGCGATTG CTTGTTATTC TGGATATAAC 
CAAGAAGATT CTGTTATTAT GAACCAATCA TCAATTGATC GTGGATTCTT TAGATCACTT 
TTCTTCCGTT CATATAGGGA TGAGGAGAAA AAGATGGGCA CACTTGTTAA AGAGGATTTT 
GGTCGCCCAG ACAGAAGTAA CACAATGGGC ATGCGCCATG GCTCTTACGA TAAATTGGAT 
GATGATGGTC TTGCACCTCC TGGCACAAGA GTTTCAGGGG AGGATGTGAT CATTGGAAAG 
ACGACTCCAA TCGCTCAGGA AGAAGCTCAG GGACAGTCTG CACGCTACAC GAGGCGTGAT 
CACAGCATAA GTTTACGTCA CAGTGAAACT GGAATGGTGG ATCAAGTTCT GCTTACAACA 
AATGCTGATG GGTTGAGGTT TGTGAAAGTA AGGGTCAGAT CTGTCCGCAT ACCTCAGATT 
GGAGACAAGT TTAGTAGTAG GCATGGTCAA AAGGGAACTG TTGGCATGAC TTACACACAA 
GAAGACATGC CTTGGACTGT GGAAGGCATC ACTCCTGATA TTATTGTCAA CCCACATGCT 
ATTCCCTCTC GAATGACAAT TGGACAGCTT ATCGAGTGTA TTATGGGAAA GGTTGCAGCA 
CACATGGGAA AGGAAGGGGA TGCCACACCA TTTACTGATG TCACTGTGGA TAATATTAGC 
AAAGCTCTTC ACAAGTGTGG GTATCAAATG CGAGGGTTTG AGACCATGTA TAATGGTCAC 
ACTGGGCGCC GGCTGTCAGC GATGATTTTC CTTGGTCCCA CTTATTATCA AAGGCTAAAA 
CATATGGTTG ATGACAAGAT CCATTCCCGT GGAAGAGGCC CTGTGCAGAT CCTCACAAGG 
CAGCCTGCTG AAGGAAGGTC ACGTGATGGT GGTCTCCGCT TTGGTGAGAT GGAACGAGAT 
TGCATGATAG CACATGGTGC CGCACACTTC TTGAAAGAGA GGCTATTTGA CCAGAGTGAC 
GCATATAGAG TCCATGTCTG TGAGCGTTGT GGGTTGATAG CTATTGCAAA TCTCAAGAAG 
AATTCTTTTG AATGCAGAGG TTGCAAGAAC AAAACCGACA TTGTTCAGGT GCACATTCCT 
TATGCCTGTA AGCTACTCTT CCAAGAGCTT ATGGCCATGG CCATAGCTCC TAGAATGCTT 
ACAAAAGATA TTAAACAGGC CAAGAGCCAG AAGAAGAAGG GAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002305136.2
CDS	146..3793
Translation	MEDLDEYDQQPMNEDMEPEEEDADEEITQEDAWAVISAYFEEKGLVRQQLDSFDEFIQNTMQEIVDESADIEIRPESQHNPGHQSDFAETIYKISFGQIYLSKPMMTESDGETATLFPKAARLRNLTYSAPLYVDVTKRVIKKGHDGEEVTETQDFTKVFIGKASIMNYLFVPIMLRSSYCTLYQNSEKDLTELGECPYDQGGYFIINGSEKVLIAQEKMSTNHVYVFKKRQPNKYAYVGEVRSMAESQNRPPSTMFVRMLSRTSAKGGSSGQYIRATLPYIRTEIPIIIVFRALGFVADKDILEHICYDFADTQMMELLRPSLEEAFVIQNQQVALDYIGKRGATVGVTREKRIKYAKEILQKEMLPHVGVGEYCETKKAYYFGYIIHRLLLCALGRRAEDDRDHYGNKRLDLAGPLLGGLFRMLFRKLTRDVRSYVQKCVDNGKDVNLQFAIKAKTITSGLKYSLATGNWGQANAAGTRAGVSQVLNRLTYASTLSHLRRLNSPIGREGKLAKPRQLHNSQWGMMCPAETPEGQACGLVKNLALMVYITVGSAAYPILEFLEEWGTENFEEISPAVIPQATKIFVNGGWVGIHRDPDMLVKTLRRLRRRVDVNTEVGVVRDIRLKELRIYTDYGRCSRPLFIVEKQKLLIRKKDIHALQQRVSHLIYNDHLFKYEGGWHDLVSKGFIEYIDTEEEETTMISMTIHDLVQARINPEEAYSDTYTHCEIHPSLILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGIYVTNYQFRMDTLAYVLYYPQKPLVTTRAMEHLHFRQLPAGINAIVAIACYSGYNQEDSVIMNQSSIDRGFFRSLFFRSYRDEEKKMGTLVKEDFGRPDRSNTMGMRHGSYDKLDDDGLAPPGTRVSGEDVIIGKTTPIAQEEAQGQSARYTRRDHSISLRHSETGMVDQVLLTTNADGLRFVKVRVRSVRIPQIGDKFSSRHGQKGTVGMTYTQEDMPWTVEGITPDIIVNPHAIPSRMTIGQLIECIMGKVAAHMGKEGDATPFTDVTVDNISKALHKCGYQMRGFETMYNGHTGRRLSAMIFLGPTYYQRLKHMVDDKIHSRGRGPVQILTRQPAEGRSRDGGLRFGEMERDCMIAHGAAHFLKERLFDQSDAYRVHVCERCGLIAIANLKKNSFECRGCKNKTDIVQVHIPYACKLLFQELMAMAIAPRMLTKDIKQAKSQKKKGA

Target ORF information:

RefSeq Version	XM_002305100.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0004s04320g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002305100.2

ORF Insert Sequence:

ATGGAAGACC TTGACGAGTA CGATCAACAG CCGATGAATG AAGATATGGA ACCAGAAGAA 
GAAGACGCTG ATGAGGAGAT CACGCAGGAA GATGCCTGGG CCGTCATTTC TGCTTATTTT 
GAAGAGAAAG GCTTGGTGAG ACAACAACTC GACTCCTTCG ATGAGTTTAT TCAAAATACG 
ATGCAGGAAA TCGTTGACGA GTCTGCTGAT ATTGAGATTC GGCCTGAAAG CCAGCACAAT 
CCGGGTCACC AGTCTGATTT TGCTGAGACT ATCTACAAGA TCAGTTTTGG CCAGATATAC 
TTGAGTAAGC CCATGATGAC AGAGTCTGAT GGAGAAACTG CAACTTTGTT TCCCAAAGCT 
GCAAGGTTGA GGAACCTGAC ATACTCGGCT CCTTTGTATG TGGATGTTAC AAAGAGGGTT 
ATAAAGAAAG GTCATGATGG TGAAGAAGTC ACTGAAACTC AAGACTTCAC CAAAGTTTTC 
ATTGGAAAGG CGAGTATAAT GAATTATTTA TTTGTCCCTA TTATGCTTCG ATCAAGTTAT 
TGCACATTAT ACCAGAATTC AGAAAAGGAT TTGACAGAGC TTGGCGAGTG TCCGTATGAT 
CAGGGTGGGT ATTTCATTAT CAATGGTAGT GAAAAGGTTC TAATTGCTCA GGAGAAGATG 
AGCACCAATC ATGTGTATGT GTTCAAGAAG AGGCAGCCAA ACAAGTATGC ATATGTAGGA 
GAAGTTCGGT CTATGGCAGA GTCTCAGAAC CGGCCACCCA GTACCATGTT TGTGCGGATG 
CTTTCTCGGA CTAGTGCTAA AGGGGGCTCT TCAGGGCAAT ACATCCGTGC TACTCTTCCA 
TACATTCGAA CTGAAATTCC GATTATCATT GTTTTTCGTG CTTTGGGTTT TGTTGCTGAC 
AAAGACATTT TGGAACACAT ATGTTATGAT TTCGCTGATA CTCAAATGAT GGAGTTGCTT 
CGGCCATCTC TGGAAGAGGC ATTTGTTATT CAAAATCAGC AGGTTGCTCT TGACTACATT 
GGAAAGCGTG GGGCAACTGT TGGTGTAACT AGGGAAAAAA GGATTAAGTA TGCCAAAGAG 
ATTCTTCAAA AAGAAATGCT TCCTCATGTT GGTGTTGGAG AATACTGCGA GACCAAAAAA 
GCTTATTACT TCGGGTATAT CATTCACCGG CTTTTACTTT GTGCACTTGG CCGGAGGGCA 
GAAGATGATA GGGATCATTA TGGAAACAAG CGGCTTGACC TTGCTGGTCC TTTACTTGGG 
GGCTTGTTTA GAATGTTATT CAGAAAGTTG ACCAGGGATG TCAGGTCTTA TGTGCAAAAG 
TGTGTTGATA ATGGGAAGGA TGTTAACCTA CAATTTGCCA TTAAAGCAAA AACCATTACC 
AGTGGTCTTA AATACTCACT TGCCACTGGG AACTGGGGAC AAGCAAATGC AGCTGGAACA 
AGAGCTGGAG TGTCACAGGT GTTAAATAGA TTGACATATG CCTCTACACT GTCACATTTA 
AGAAGATTAA ATTCTCCAAT TGGGCGTGAA GGGAAATTGG CAAAACCAAG GCAGCTACAT 
AATTCACAAT GGGGAATGAT GTGTCCTGCT GAAACACCTG AAGGACAAGC TTGTGGATTA 
GTGAAGAATC TCGCATTGAT GGTATACATT ACAGTTGGAT CAGCTGCATA TCCCATCCTG 
GAGTTTTTAG AAGAATGGGG TACCGAGAAT TTTGAGGAAA TATCCCCTGC AGTTATTCCC 
CAAGCAACAA AAATTTTTGT CAATGGTGGC TGGGTGGGTA TCCATCGTGA TCCTGATATG 
TTGGTGAAGA CATTGAGACG GCTGCGGAGA CGGGTTGATG TCAATACTGA AGTTGGGGTT 
GTTAGAGATA TTCGCTTGAA AGAGTTGCGG ATTTACACTG ACTATGGCCG GTGCAGTCGT 
CCTCTATTCA TTGTGGAGAA ACAAAAGCTT CTTATAAGGA AGAAGGACAT CCATGCACTA 
CAACAGAGGG TGAGTCATTT AATTTATAAT GATCATCTTT TCAAATATGA GGGTGGCTGG 
CATGATCTTG TGTCAAAGGG ATTCATTGAA TATATTGACA CTGAGGAAGA AGAGACCACT 
ATGATATCCA TGACCATACA TGATCTTGTA CAAGCGAGGA TTAATCCAGA GGAAGCATAT 
TCTGATACTT ACACCCATTG TGAGATCCAC CCATCATTGA TTTTAGGTGT TTGTGCTTCT 
ATCATTCCTT TTCCTGACCA TAACCAGTCC CCACGTAACA CTTACCAATC TGCTATGGGA 
AAGCAAGCCA TGGGAATTTA TGTCACCAAT TACCAATTTC GAATGGATAC GCTGGCTTAT 
GTTCTCTATT ATCCCCAAAA GCCACTTGTT ACTACAAGAG CCATGGAGCA TCTTCATTTT 
CGGCAACTTC CTGCTGGCAT TAATGCTATT GTTGCGATTG CTTGTTATTC TGGATATAAC 
CAAGAAGATT CTGTTATTAT GAACCAATCA TCAATTGATC GTGGATTCTT TAGATCACTT 
TTCTTCCGTT CATATAGGGA TGAGGAGAAA AAGATGGGCA CACTTGTTAA AGAGGATTTT 
GGTCGCCCAG ACAGAAGTAA CACAATGGGC ATGCGCCATG GCTCTTACGA TAAATTGGAT 
GATGATGGTC TTGCACCTCC TGGCACAAGA GTTTCAGGGG AGGATGTGAT CATTGGAAAG 
ACGACTCCAA TCGCTCAGGA AGAAGCTCAG GGACAGTCTG CACGCTACAC GAGGCGTGAT 
CACAGCATAA GTTTACGTCA CAGTGAAACT GGAATGGTGG ATCAAGTTCT GCTTACAACA 
AATGCTGATG GGTTGAGGTT TGTGAAAGTA AGGGTCAGAT CTGTCCGCAT ACCTCAGATT 
GGAGACAAGT TTAGTAGTAG GCATGGTCAA AAGGGAACTG TTGGCATGAC TTACACACAA 
GAAGACATGC CTTGGACTGT GGAAGGCATC ACTCCTGATA TTATTGTCAA CCCACATGCT 
ATTCCCTCTC GAATGACAAT TGGACAGCTT ATCGAGTGTA TTATGGGAAA GGTTGCAGCA 
CACATGGGAA AGGAAGGGGA TGCCACACCA TTTACTGATG TCACTGTGGA TAATATTAGC 
AAAGCTCTTC ACAAGTGTGG GTATCAAATG CGAGGGTTTG AGACCATGTA TAATGGTCAC 
ACTGGGCGCC GGCTGTCAGC GATGATTTTC CTTGGTCCCA CTTATTATCA AAGGCTAAAA 
CATATGGTTG ATGACAAGAT CCATTCCCGT GGAAGAGGCC CTGTGCAGAT CCTCACAAGG 
CAGCCTGCTG AAGGAAGGTC ACGTGATGGT GGTCTCCGCT TTGGTGAGAT GGAACGAGAT 
TGCATGATAG CACATGGTGC CGCACACTTC TTGAAAGAGA GGCTATTTGA CCAGAGTGAC 
GCATATAGAG TCCATGTCTG TGAGCGTTGT GGGTTGATAG CTATTGCAAA TCTCAAGAAG 
AATTCTTTTG AATGCAGAGG TTGCAAGAAC AAAACCGACA TTGTTCAGGT GCACATTCCT 
TATGCCTGTA AGCTACTCTT CCAAGAGCTT ATGGCCATGG CCATAGCTCC TAGAATGCTT 
ACAAAAGATA TTAAACAGGC CAAGAGCCAG AAGAAGAAGG GAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0004s04320g ( XM_024599156.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024454924.1 Populus trichocarpa DNA-directed RNA polymerase II subunit RPB2 (LOC7477407), mRNA.

Price & Availability↑

CloneID	OPa427900
Clone ID Related Accession (Same CDS sequence)	XM_024599156.1
Accession Version	XM_024599156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	DNA-directed RNA polymerase II subunit RPB2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037288.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACC TTGACGAGTA CGATCAACAG CCGATGAATG AAGATATGGA ACCAGAAGAA 
GAAGACGCTG ATGAGGAGAT CACGCAGGAA GATGCCTGGG CCGTCATTTC TGCTTATTTT 
GAAGAGAAAG GCTTGGTGAG ACAACAACTC GACTCCTTCG ATGAGTTTAT TCAAAATACG 
ATGCAGGAAA TCGTTGACGA GTCTGCTGAT ATTGAGATTC GGCCTGAAAG CCAGCACAAT 
CCGGGTCACC AGTCTGATTT TGCTGAGACT ATCTACAAGA TCAGTTTTGG CCAGATATAC 
TTGAGTAAGC CCATGATGAC AGAGTCTGAT GGAGAAACTG CAACTTTGTT TCCCAAAGCT 
GCAAGGTTGA GGAACCTGAC ATACTCGGCT CCTTTGTATG TGGATGTTAC AAAGAGGGTT 
ATAAAGAAAG GTCATGATGG TGAAGAAGTC ACTGAAACTC AAGACTTCAC CAAAGTTTTC 
ATTGGAAAGG TCCCTATTAT GCTTCGATCA AGTTATTGCA CATTATACCA GAATTCAGAA 
AAGGATTTGA CAGAGCTTGG CGAGTGTCCG TATGATCAGG GTGGGTATTT CATTATCAAT 
GGTAGTGAAA AGGTTCTAAT TGCTCAGGAG AAGATGAGCA CCAATCATGT GTATGTGTTC 
AAGAAGAGGC AGCCAAACAA GTATGCATAT GTAGGAGAAG TTCGGTCTAT GGCAGAGTCT 
CAGAACCGGC CACCCAGTAC CATGTTTGTG CGGATGCTTT CTCGGACTAG TGCTAAAGGG 
GGCTCTTCAG GGCAATACAT CCGTGCTACT CTTCCATACA TTCGAACTGA AATTCCGATT 
ATCATTGTTT TTCGTGCTTT GGGTTTTGTT GCTGACAAAG ACATTTTGGA ACACATATGT 
TATGATTTCG CTGATACTCA AATGATGGAG TTGCTTCGGC CATCTCTGGA AGAGGCATTT 
GTTATTCAAA ATCAGCAGGT TGCTCTTGAC TACATTGGAA AGCGTGGGGC AACTGTTGGT 
GTAACTAGGG AAAAAAGGAT TAAGTATGCC AAAGAGATTC TTCAAAAAGA AATGCTTCCT 
CATGTTGGTG TTGGAGAATA CTGCGAGACC AAAAAAGCTT ATTACTTCGG GTATATCATT 
CACCGGCTTT TACTTTGTGC ACTTGGCCGG AGGGCAGAAG ATGATAGGGA TCATTATGGA 
AACAAGCGGC TTGACCTTGC TGGTCCTTTA CTTGGGGGCT TGTTTAGAAT GTTATTCAGA 
AAGTTGACCA GGGATGTCAG GTCTTATGTG CAAAAGTGTG TTGATAATGG GAAGGATGTT 
AACCTACAAT TTGCCATTAA AGCAAAAACC ATTACCAGTG GTCTTAAATA CTCACTTGCC 
ACTGGGAACT GGGGACAAGC AAATGCAGCT GGAACAAGAG CTGGAGTGTC ACAGGTGTTA 
AATAGATTGA CATATGCCTC TACACTGTCA CATTTAAGAA GATTAAATTC TCCAATTGGG 
CGTGAAGGGA AATTGGCAAA ACCAAGGCAG CTACATAATT CACAATGGGG AATGATGTGT 
CCTGCTGAAA CACCTGAAGG ACAAGCTTGT GGATTAGTGA AGAATCTCGC ATTGATGGTA 
TACATTACAG TTGGATCAGC TGCATATCCC ATCCTGGAGT TTTTAGAAGA ATGGGGTACC 
GAGAATTTTG AGGAAATATC CCCTGCAGTT ATTCCCCAAG CAACAAAAAT TTTTGTCAAT 
GGTGGCTGGG TGGGTATCCA TCGTGATCCT GATATGTTGG TGAAGACATT GAGACGGCTG 
CGGAGACGGG TTGATGTCAA TACTGAAGTT GGGGTTGTTA GAGATATTCG CTTGAAAGAG 
TTGCGGATTT ACACTGACTA TGGCCGGTGC AGTCGTCCTC TATTCATTGT GGAGAAACAA 
AAGCTTCTTA TAAGGAAGAA GGACATCCAT GCACTACAAC AGAGGGAAAC CCCAGATGAG 
GGTGGCTGGC ATGATCTTGT GTCAAAGGGA TTCATTGAAT ATATTGACAC TGAGGAAGAA 
GAGACCACTA TGATATCCAT GACCATACAT GATCTTGTAC AAGCGAGGAT TAATCCAGAG 
GAAGCATATT CTGATACTTA CACCCATTGT GAGATCCACC CATCATTGAT TTTAGGTGTT 
TGTGCTTCTA TCATTCCTTT TCCTGACCAT AACCAGTCCC CACGTAACAC TTACCAATCT 
GCTATGGGAA AGCAAGCCAT GGGAATTTAT GTCACCAATT ACCAATTTCG AATGGATACG 
CTGGCTTATG TTCTCTATTA TCCCCAAAAG CCACTTGTTA CTACAAGAGC CATGGAGCAT 
CTTCATTTTC GGCAACTTCC TGCTGGCATT AATGCTATTG TTGCGATTGC TTGTTATTCT 
GGATATAACC AAGAAGATTC TGTTATTATG AACCAATCAT CAATTGATCG TGGATTCTTT 
AGATCACTTT TCTTCCGTTC ATATAGGGAT GAGGAGAAAA AGATGGGCAC ACTTGTTAAA 
GAGGATTTTG GTCGCCCAGA CAGAAGTAAC ACAATGGGCA TGCGCCATGG CTCTTACGAT 
AAATTGGATG ATGATGGTCT TGCACCTCCT GGCACAAGAG TTTCAGGGGA GGATGTGATC 
ATTGGAAAGA CGACTCCAAT CGCTCAGGAA GAAGCTCAGG GACAGTCTGC ACGCTACACG 
AGGCGTGATC ACAGCATAAG TTTACGTCAC AGTGAAACTG GAATGGTGGA TCAAGTTCTG 
CTTACAACAA ATGCTGATGG GTTGAGGTTT GTGAAAGTAA GGGTCAGATC TGTCCGCATA 
CCTCAGATTG GAGACAAGTT TAGTAGTAGG CATGGTCAAA AGGGAACTGT TGGCATGACT 
TACACACAAG AAGACATGCC TTGGACTGTG GAAGGCATCA CTCCTGATAT TATTGTCAAC 
CCACATGCTA TTCCCTCTCG AATGACAATT GGACAGCTTA TCGAGTGTAT TATGGGAAAG 
GTTGCAGCAC ACATGGGAAA GGAAGGGGAT GCCACACCAT TTACTGATGT CACTGTGGAT 
AATATTAGCA AAGCTCTTCA CAAGTGTGGG TATCAAATGC GAGGGTTTGA GACCATGTAT 
AATGGTCACA CTGGGCGCCG GCTGTCAGCG ATGATTTTCC TTGGTCCCAC TTATTATCAA 
AGGCTAAAAC ATATGGTTGA TGACAAGATC CATTCCCGTG GAAGAGGCCC TGTGCAGATC 
CTCACAAGGC AGCCTGCTGA AGGAAGGTCA CGTGATGGTG GTCTCCGCTT TGGTGAGATG 
GAACGAGATT GCATGATAGC ACATGGTGCC GCACACTTCT TGAAAGAGAG GCTATTTGAC 
CAGAGTGACG CATATAGAGT CCATGTCTGT GAGCGTTGTG GGTTGATAGC TATTGCAAAT 
CTCAAGAAGA ATTCTTTTGA ATGCAGAGGT TGCAAGAACA AAACCGACAT TGTTCAGGTG 
CACATTCCTT ATGCCTGTAA GCTACTCTTC CAAGAGCTTA TGGCCATGGC CATAGCTCCT 
AGAATGCTTA CAAAAGATAT TAAACAGGCC AAGAGCCAGA AGAAGAAGGG AGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024454924.1
CDS	164..3760
Translation	MEDLDEYDQQPMNEDMEPEEEDADEEITQEDAWAVISAYFEEKGLVRQQLDSFDEFIQNTMQEIVDESADIEIRPESQHNPGHQSDFAETIYKISFGQIYLSKPMMTESDGETATLFPKAARLRNLTYSAPLYVDVTKRVIKKGHDGEEVTETQDFTKVFIGKVPIMLRSSYCTLYQNSEKDLTELGECPYDQGGYFIINGSEKVLIAQEKMSTNHVYVFKKRQPNKYAYVGEVRSMAESQNRPPSTMFVRMLSRTSAKGGSSGQYIRATLPYIRTEIPIIIVFRALGFVADKDILEHICYDFADTQMMELLRPSLEEAFVIQNQQVALDYIGKRGATVGVTREKRIKYAKEILQKEMLPHVGVGEYCETKKAYYFGYIIHRLLLCALGRRAEDDRDHYGNKRLDLAGPLLGGLFRMLFRKLTRDVRSYVQKCVDNGKDVNLQFAIKAKTITSGLKYSLATGNWGQANAAGTRAGVSQVLNRLTYASTLSHLRRLNSPIGREGKLAKPRQLHNSQWGMMCPAETPEGQACGLVKNLALMVYITVGSAAYPILEFLEEWGTENFEEISPAVIPQATKIFVNGGWVGIHRDPDMLVKTLRRLRRRVDVNTEVGVVRDIRLKELRIYTDYGRCSRPLFIVEKQKLLIRKKDIHALQQRETPDEGGWHDLVSKGFIEYIDTEEEETTMISMTIHDLVQARINPEEAYSDTYTHCEIHPSLILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGIYVTNYQFRMDTLAYVLYYPQKPLVTTRAMEHLHFRQLPAGINAIVAIACYSGYNQEDSVIMNQSSIDRGFFRSLFFRSYRDEEKKMGTLVKEDFGRPDRSNTMGMRHGSYDKLDDDGLAPPGTRVSGEDVIIGKTTPIAQEEAQGQSARYTRRDHSISLRHSETGMVDQVLLTTNADGLRFVKVRVRSVRIPQIGDKFSSRHGQKGTVGMTYTQEDMPWTVEGITPDIIVNPHAIPSRMTIGQLIECIMGKVAAHMGKEGDATPFTDVTVDNISKALHKCGYQMRGFETMYNGHTGRRLSAMIFLGPTYYQRLKHMVDDKIHSRGRGPVQILTRQPAEGRSRDGGLRFGEMERDCMIAHGAAHFLKERLFDQSDAYRVHVCERCGLIAIANLKKNSFECRGCKNKTDIVQVHIPYACKLLFQELMAMAIAPRMLTKDIKQAKSQKKKGA

Target ORF information:

RefSeq Version	XM_024599156.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa DNA-directed RNA polymerase II subunit RPB2 (LOC7477407), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024599156.1

ORF Insert Sequence:

ATGGAAGACC TTGACGAGTA CGATCAACAG CCGATGAATG AAGATATGGA ACCAGAAGAA 
GAAGACGCTG ATGAGGAGAT CACGCAGGAA GATGCCTGGG CCGTCATTTC TGCTTATTTT 
GAAGAGAAAG GCTTGGTGAG ACAACAACTC GACTCCTTCG ATGAGTTTAT TCAAAATACG 
ATGCAGGAAA TCGTTGACGA GTCTGCTGAT ATTGAGATTC GGCCTGAAAG CCAGCACAAT 
CCGGGTCACC AGTCTGATTT TGCTGAGACT ATCTACAAGA TCAGTTTTGG CCAGATATAC 
TTGAGTAAGC CCATGATGAC AGAGTCTGAT GGAGAAACTG CAACTTTGTT TCCCAAAGCT 
GCAAGGTTGA GGAACCTGAC ATACTCGGCT CCTTTGTATG TGGATGTTAC AAAGAGGGTT 
ATAAAGAAAG GTCATGATGG TGAAGAAGTC ACTGAAACTC AAGACTTCAC CAAAGTTTTC 
ATTGGAAAGG TCCCTATTAT GCTTCGATCA AGTTATTGCA CATTATACCA GAATTCAGAA 
AAGGATTTGA CAGAGCTTGG CGAGTGTCCG TATGATCAGG GTGGGTATTT CATTATCAAT 
GGTAGTGAAA AGGTTCTAAT TGCTCAGGAG AAGATGAGCA CCAATCATGT GTATGTGTTC 
AAGAAGAGGC AGCCAAACAA GTATGCATAT GTAGGAGAAG TTCGGTCTAT GGCAGAGTCT 
CAGAACCGGC CACCCAGTAC CATGTTTGTG CGGATGCTTT CTCGGACTAG TGCTAAAGGG 
GGCTCTTCAG GGCAATACAT CCGTGCTACT CTTCCATACA TTCGAACTGA AATTCCGATT 
ATCATTGTTT TTCGTGCTTT GGGTTTTGTT GCTGACAAAG ACATTTTGGA ACACATATGT 
TATGATTTCG CTGATACTCA AATGATGGAG TTGCTTCGGC CATCTCTGGA AGAGGCATTT 
GTTATTCAAA ATCAGCAGGT TGCTCTTGAC TACATTGGAA AGCGTGGGGC AACTGTTGGT 
GTAACTAGGG AAAAAAGGAT TAAGTATGCC AAAGAGATTC TTCAAAAAGA AATGCTTCCT 
CATGTTGGTG TTGGAGAATA CTGCGAGACC AAAAAAGCTT ATTACTTCGG GTATATCATT 
CACCGGCTTT TACTTTGTGC ACTTGGCCGG AGGGCAGAAG ATGATAGGGA TCATTATGGA 
AACAAGCGGC TTGACCTTGC TGGTCCTTTA CTTGGGGGCT TGTTTAGAAT GTTATTCAGA 
AAGTTGACCA GGGATGTCAG GTCTTATGTG CAAAAGTGTG TTGATAATGG GAAGGATGTT 
AACCTACAAT TTGCCATTAA AGCAAAAACC ATTACCAGTG GTCTTAAATA CTCACTTGCC 
ACTGGGAACT GGGGACAAGC AAATGCAGCT GGAACAAGAG CTGGAGTGTC ACAGGTGTTA 
AATAGATTGA CATATGCCTC TACACTGTCA CATTTAAGAA GATTAAATTC TCCAATTGGG 
CGTGAAGGGA AATTGGCAAA ACCAAGGCAG CTACATAATT CACAATGGGG AATGATGTGT 
CCTGCTGAAA CACCTGAAGG ACAAGCTTGT GGATTAGTGA AGAATCTCGC ATTGATGGTA 
TACATTACAG TTGGATCAGC TGCATATCCC ATCCTGGAGT TTTTAGAAGA ATGGGGTACC 
GAGAATTTTG AGGAAATATC CCCTGCAGTT ATTCCCCAAG CAACAAAAAT TTTTGTCAAT 
GGTGGCTGGG TGGGTATCCA TCGTGATCCT GATATGTTGG TGAAGACATT GAGACGGCTG 
CGGAGACGGG TTGATGTCAA TACTGAAGTT GGGGTTGTTA GAGATATTCG CTTGAAAGAG 
TTGCGGATTT ACACTGACTA TGGCCGGTGC AGTCGTCCTC TATTCATTGT GGAGAAACAA 
AAGCTTCTTA TAAGGAAGAA GGACATCCAT GCACTACAAC AGAGGGAAAC CCCAGATGAG 
GGTGGCTGGC ATGATCTTGT GTCAAAGGGA TTCATTGAAT ATATTGACAC TGAGGAAGAA 
GAGACCACTA TGATATCCAT GACCATACAT GATCTTGTAC AAGCGAGGAT TAATCCAGAG 
GAAGCATATT CTGATACTTA CACCCATTGT GAGATCCACC CATCATTGAT TTTAGGTGTT 
TGTGCTTCTA TCATTCCTTT TCCTGACCAT AACCAGTCCC CACGTAACAC TTACCAATCT 
GCTATGGGAA AGCAAGCCAT GGGAATTTAT GTCACCAATT ACCAATTTCG AATGGATACG 
CTGGCTTATG TTCTCTATTA TCCCCAAAAG CCACTTGTTA CTACAAGAGC CATGGAGCAT 
CTTCATTTTC GGCAACTTCC TGCTGGCATT AATGCTATTG TTGCGATTGC TTGTTATTCT 
GGATATAACC AAGAAGATTC TGTTATTATG AACCAATCAT CAATTGATCG TGGATTCTTT 
AGATCACTTT TCTTCCGTTC ATATAGGGAT GAGGAGAAAA AGATGGGCAC ACTTGTTAAA 
GAGGATTTTG GTCGCCCAGA CAGAAGTAAC ACAATGGGCA TGCGCCATGG CTCTTACGAT 
AAATTGGATG ATGATGGTCT TGCACCTCCT GGCACAAGAG TTTCAGGGGA GGATGTGATC 
ATTGGAAAGA CGACTCCAAT CGCTCAGGAA GAAGCTCAGG GACAGTCTGC ACGCTACACG 
AGGCGTGATC ACAGCATAAG TTTACGTCAC AGTGAAACTG GAATGGTGGA TCAAGTTCTG 
CTTACAACAA ATGCTGATGG GTTGAGGTTT GTGAAAGTAA GGGTCAGATC TGTCCGCATA 
CCTCAGATTG GAGACAAGTT TAGTAGTAGG CATGGTCAAA AGGGAACTGT TGGCATGACT 
TACACACAAG AAGACATGCC TTGGACTGTG GAAGGCATCA CTCCTGATAT TATTGTCAAC 
CCACATGCTA TTCCCTCTCG AATGACAATT GGACAGCTTA TCGAGTGTAT TATGGGAAAG 
GTTGCAGCAC ACATGGGAAA GGAAGGGGAT GCCACACCAT TTACTGATGT CACTGTGGAT 
AATATTAGCA AAGCTCTTCA CAAGTGTGGG TATCAAATGC GAGGGTTTGA GACCATGTAT 
AATGGTCACA CTGGGCGCCG GCTGTCAGCG ATGATTTTCC TTGGTCCCAC TTATTATCAA 
AGGCTAAAAC ATATGGTTGA TGACAAGATC CATTCCCGTG GAAGAGGCCC TGTGCAGATC 
CTCACAAGGC AGCCTGCTGA AGGAAGGTCA CGTGATGGTG GTCTCCGCTT TGGTGAGATG 
GAACGAGATT GCATGATAGC ACATGGTGCC GCACACTTCT TGAAAGAGAG GCTATTTGAC 
CAGAGTGACG CATATAGAGT CCATGTCTGT GAGCGTTGTG GGTTGATAGC TATTGCAAAT 
CTCAAGAAGA ATTCTTTTGA ATGCAGAGGT TGCAAGAACA AAACCGACAT TGTTCAGGTG 
CACATTCCTT ATGCCTGTAA GCTACTCTTC CAAGAGCTTA TGGCCATGGC CATAGCTCCT 
AGAATGCTTA CAAAAGATAT TAAACAGGCC AAGAGCCAGA AGAAGAAGGG AGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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POPTR_0004s04320g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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