LOC7461259 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC7461259
Entrez Gene ID	7461259
Full Name	hypothetical protein
Synonyms	POPTRDRAFT_778228
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002323929.2	XP_002323965.1	hypothetical protein
XM_024588266.1	XP_024444034.1	ATP-dependent RNA helicase DEAH11, chloroplastic

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC7461259 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC7461259 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa33857	XM_002323929.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0017s07680g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa445966	XM_024588266.1 Latest version!	Populus trichocarpa ATP-dependent RNA helicase DEAH11, chloroplastic (LOC7461259), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC7461259 ( XM_002323929.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002323965.1 Populus trichocarpa hypothetical protein (POPTR_0017s07680g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa33857
Clone ID Related Accession (Same CDS sequence)	XM_002323929.2
Accession Version	XM_002323929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008483). On Dec 16, 2013 this sequence version replaced XM_002323929.1. COMPLETENESS: incomplete on the 3' end.

ATGATCACCA GCAATAACCA CCACCCCTCC ACACGCCACC ACCACCACTC CCAACACCGT 
CAGTCGCAGC ATTTCAACCG TCACCGGAAC TTCCGAAACC ACCAAAACCA AAGCCTTTTT 
GTAGTCAGGC TTTTGTCCAA CCACCGTAAT AATAACCGCA CGCAATCCCC ACTCGAAACC 
CTAATTTCCC AATGTAACCC TAAACCTGAC AAATCCGATA CAAACCCCAC GTCCGCCGTT 
GCTGCTCGTT TGTTTTTCCA CGACCAGAGC GATGCTATCG CCGCCGTCGT CTTCCTCTGG 
GAACGGAGGC TGGCTGGGGA TCATGTGTAT ACTCCGGTGA CGGATTTTGA TGTAAATGAA 
GGGGACTTGA ATGAGAGGAT TAGAGGGGAG GTAGTGAAGA AATTGGAGAG AAAGATTGAA 
AATTTGGCCG TGGAAATTGG TAAATTTACG AGCTTTTTCA AGCGGCCGAA AGGGGTTAGG 
GTTTATAGTG AGAATAAGGT GAAGAAGGAG GCGTTGAGGG TGGAAATGGA GGTGGTTGTG 
AAGAGGGTGG AGGAGTTTAG GAAGGGAATG AGGTGTTTGA TGGATTGCAT TGAAGGGAAG 
GAGATTGGTG ATTTGGGGGT TTTGAGGGTT TATGATGAGG GTAATGGGAG AAAGATGGGA 
ATTTTTTATT ATTGGAGCAG GATTCATTTT CTGATATTGA GAGAGTGTAG AAGGGTAGAA 
AATGGGTTGC CTGTTTATGG GTTTAGGAGT GAGTTTTTGA AGATGCTGCG TTCACAGCAG 
GAATATGCGT TGATTGTTTC TGATTTGATT TTGTGCTTCA AGCTTAGTGG TATATGCTTC 
TTGTATTTAA AGGAGTATTA TGATTTGAAA TTCGACTTTA ATCTTAGTTT GTGTGCTTAT 
GCATATTCTT GTATGATCTC TTATGAATGG TTGGTGATGG TCTTGATTGG AGAAACAGGT 
TCAGGGAAGA GTACGCAGTT GGCACAATTT ATTGCTGATT CAGGAGTTGC ATCAAGTGGT 
TCAATTTTGT GCACTCAACC CCGCAAAATT GCTGCTATAT CCCTGGGAAA AAGGGTCGGT 
GAAGAATGCA ATGGATGTTA TGAAGATAAT TCAATTATCT GCTACCCATC TTATTCATCT 
TCCCAGCAGT TTGGTTCGAA GGTAATATAT ATGACTGATC ACTGTCTACT GCAAAACCTC 
ATGAAAGATA AAAATTTGTT TGGAGTTTCG TGCATTATTG TAGATGAGGC TCATGAAAGA 
AGCTTGAATA CTGATCTTCT TTTGGGCTTG CTAAAAGAAT TACTTCAGGA AAGGCCTGAC 
TTACAGCTAA TTATAATGTC TGCAACAGTT GATGCAAGCA AATTATCCAG TTATTTCTTT 
GGCTGTGGAA CATTTCATGT GTTGGGGAGA AGCTTTCCTG TTGAAATCAA ATATGCTCCT 
GCTGCATCTA GAGAGTCCCT TGACCCCTTG CCGAGCTCCA ATAATGCAGC TCCATATGTT 
TGTGATGTTG TTAAGATGGC AACGGAAATT CATGCAGCAG AGGAGGATGG GGCAATACTT 
GCTTTTTTGA CTTCACAGGC AGAAGTAGAA TGGGCATGTG AAAAGTTTCA GTCTCCCTCT 
GCAATTGCGC TCCCATTACA TGGGAAGCTT TTCCATGAAG AGCAATGTCG TGTTTTTCAG 
AATTACCCAG GAAAGAGAAA AGTTGTTTTT GCTACAAATT TAGCGGAAAC ATCCATAACA 
ATTCCAGGGG TCAAGTACGT GGTTGATTCT GGTTTAGTTA AAGATAGCAG GTTTGAATCT 
TCATCTGGTA TGAATGTGCT CAGAGTTTCC AAGATCAGCC AAAGTTCTGC AAATCAACGA 
GCTGGCCGTG CTGGTAGAAC AGATCCTGGC AAGTGTTACC GGTTGTACTC GGTTTCTGAT 
TATCAGTCAA TGGATTTGCA TCAGGAACCT GAAATTTGTA AAGTTCATCT TGGCATTGCA 
GTTTTGAGGA TTCTTGCCTC TGGTATTAAG AATGTGCTAG AGTTTGATTT TATTGATGCT 
CCAAGTGTGG ATGCAATCAA CAAGGCAATC AGGAACCTTG TTCAGTTAGG TGCTGTTGCC 
TGGAAACATG ATGCTTTTGT ATTAACTGCT GATGGACACT ACTTAGTTAA ATTGGGTATG 
GAGCCTCGAC TTGGGAAAAT TATTCTTGAA AGTCTCCGTT ATGGTTTGCG CAAGGAGGGA 
GTTGTCCTTG CTGCAGCTAT GGCAAATGCC AGTAACATTT TTTGCAGAGT GGGTACTTAT 
GATGAGAAAC TGAAGTCTGA TTGCCTTAAG GTCCGGTTCT GCCATCACGA TGGAGATCTC 
TTCACTTTGC TCTCCGTCTA CAGGGAATGG GAGAGTTTAC GGCAAGAGAA TAGAAATAAA 
TGGTGTTGGG AAAATAGAAT CAATGCAAAA ACTATGCGAA GATGCAGAGA TACAGTATTG 
GAGTTGGAAA ACTGTTTGAA AAATGAACTC AATATCATTA TTCCAACTTA CTGGCTTTGG 
GATCCTCTTG TTGCCTCTGT GCATGATGAA AACATGAAGA AGATTATACT TTCTTCACTA 
GCAGATAATG TAGCTATGTA TTCTGGATAT GATCGCCTTG GTTATGAGGT TGTTTTAAGT 
GGTGAATACT TCCAACTTCA CCCTTCATGT TCATTACAAG TGTATAACCA GAAGCCACAT 
TGGGTTGTCT TTGCTGAACT CTTGTCCATA TCAAGTCAAT ATTTGGTTTG TGTTACAGCA 
ATTGACTTTG ACAGTTTATC TACCTTCATA CATCCTTTAT TTGATGTCTC AAAGATGGAA 
TCTAGAAAAC TGCAGTTGAG GGTGATAAAA GGATTTGGAG GTGTTGCTCT GAAACGGTTT 
TGTGGAAAGT CAAATAGTAG CTTGATAGCA CTTGTTTCTC GCATGCGTGC TATTTACATG 
GATGAACGCA TTGGAATTGA AATCAATGTG GGTGACAACG AGATTCAATT GTTTGCATCG 
TCCAAAGATA TTGAAAAGAT TTATGAATAT GTCAATAATG CGTTAAGATA TGAAACAAAG 
TGGTTAAGGA ATGAATGCTT GGAGAAGTGC TTATATCATG AGGTACGGGC TGGTGCTTCC 
CCCCCTGTTG CACTTGTTGG AGCTGGTGCT GAAATTAAGC ATCTGGAGTT GGGAAATAGA 
TGTTTAACAG TGGATGTGCA CCTTTCCAAT GTGAATGTGG TAGATGACAA GGAAGTTTTG 
ACATTCTTAG AGAAATCTGT ATCTGGAATT TGTGGTTATA ACAAATTCAC GGGCATTGGA 
CAGCATGGTG GTGATGCAGA AAGGTGGGGC AGGGTGTCAT TCCTTACTCC TGAAGCAGCT 
AGAAAAGCTC TATATTTTAA TGGATCCGAG TTATGTGGAT GTGTACTTAA GTTGTCCCTG 
TCTCGGTCAT CTGTTGGAGG TATTCGAAAG TCATCATTTG CTGCCGTAAA AGCAAAAATC 
AGTTGGCCAC GCCGGTATAG CAAAGGGTAT GCAATTGTCA GATGTGAAAG GAATGATGCC 
CAGTTCATTG TTGATGATTG CTTTAATGTT CTGATTGGAG GCAGGTTTGT TCAGTGTCAG 
ACCAGCACAA GAGACATGAA CTCTGTAGTT ATCAGAGGAC TGGATAAAGA AACTTCTGAA 
GCAGAAATTT TAGAAGTTCT GCACAAGACA ACAAATAGGA GAATTTTGGA TGTATTCCTA 
ATCAGAGGAG ATGAAGCAAA TAATCATTCT GTTGATGCTT TTGAGCAAGC AATTCTCAAG 
GAAATTGCTC CTTTCATGCC TAGCCAGGGC CCTTTGAGCA ATTACTGCCA TGTTCAGGTC 
TTTGCCCCTG AGCCAAAGGA TAGTTTCATG AAGGCTTGGA TCACTTTTGA TGGAAAATTA 
CATTTGGAGG CAGCTAAAGC TCTGCAACAT ATGCAAGGAA AAGCACTTGC TGGTTGTTTT 
TCATGGCAGA AGATGCAATG CCAGCAGGTG TTCCATAGCT CTGCATCATG TTCTGCTTCT 
GTTTATGCAT TCATTGAGAG GCAGCTGAAT ATTTTACTGA AAAGCTTCAA GTTTCGACCT 
GGTGTGTGTT GCAATTTAGA AAGGAATGAG AATGGCTCTT ATAGAGTGAA GATATCTGCT 
AATGCAACAA AGACAGTAGC TGAGTTGAGA AGGCCGCTGG AGCAACTGAT GAATGGAAAG 
AAGCTTCTCT TTTCCAAAGA TGGCATCATG CTTATGAAGT CACTGCAGCA AGAGATGGGA 
ACATATATTC TCTTTGATAG GCAGAACCTC ACTGTTAGGA TCTTTGGCCC CGAGAAGAAA 
GTTGCTCTTA CTGAACAGAA GTTGATAGCA TCTCTTCTAG CCCTCCATGA TAAGGAACAA 
ACAGATATCC GCCTTCGAGG TGGAGCAATG CCGTATGATC TTATGAAAAA GGTGGTGGAG 
AAGTTTGGTC CTGACTTGCA TGTGCTCAAA GAGACGTTCC CTGAGGCTGA ATTTATGCTT 
AATACAAGGC GACATGTTAT TTCTTTTTCT GGAAAAAAGG ATCTGAGACT CCAAGTGGAG 
CAGATGATTC GTGACTTTGT GCGGTCTGTC GGTGTTAATG GATCCATCAA ACGATATGAA 
GATGACAATA TTGCCTGCCC TATTTGCCTT TGTGAGGTTG AGGACTGTTA CCAGCTTGAG 
GCTTGTGGGC ATAAATTCTG CCAGTCATGC TTGGTTGAAC AGTTAGAGTC AGCAATGAGA 
GGACGTGATG GCTTCCCTGT AGGTTGTGCT CATGAAGGTT GTGGCATGCA CATTTGGCTT 
ACAGATCTGA AGTCTCTTTT ACCATGTGAG AAGTTAGAGG ATCTCTTCAG GGCCTCCTTG 
TCAGCCTTTG TGGCATCTAG TGGTGGAACT TACAGATTTT GCCCATCTCC TGATTGCCCT 
TCAGTATATC ACGTAGCAAG TGGGATGGTT GGTGATCTAT TTGTTTGTGG AGCATGCTAT 
GCCGAGACAT GTACAAGGTG CCATGTGGAG TATCATCCAT TCGTATCATG TGAAAAATAC 
AAGGAGCTCA AGGAGGATCC TGACATGTCC TTGAAGGAAT GGTGCAAGGG AAAAGAGCAT 
GTTAGAAACT GCCCAGTATG TGGATACACA ATTGAGAAGG TAGATGGCTG CAACCACATT 
GAGTGCAGAT GTGGGAAACA CATCTGCTGG GTCTGCTTGG AGGTCTTTAT GAGCGGTGAT 
GACTGCTATG CGCACTTGAG GTCTGTACAT CCTGCTACAT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002323965.1
CDS	146..5410
Translation	MITSNNHHPSTRHHHHSQHRQSQHFNRHRNFRNHQNQSLFVVRLLSNHRNNNRTQSPLETLISQCNPKPDKSDTNPTSAVAARLFFHDQSDAIAAVVFLWERRLAGDHVYTPVTDFDVNEGDLNERIRGEVVKKLERKIENLAVEIGKFTSFFKRPKGVRVYSENKVKKEALRVEMEVVVKRVEEFRKGMRCLMDCIEGKEIGDLGVLRVYDEGNGRKMGIFYYWSRIHFLILRECRRVENGLPVYGFRSEFLKMLRSQQEYALIVSDLILCFKLSGICFLYLKEYYDLKFDFNLSLCAYAYSCMISYEWLVMVLIGETGSGKSTQLAQFIADSGVASSGSILCTQPRKIAAISLGKRVGEECNGCYEDNSIICYPSYSSSQQFGSKVIYMTDHCLLQNLMKDKNLFGVSCIIVDEAHERSLNTDLLLGLLKELLQERPDLQLIIMSATVDASKLSSYFFGCGTFHVLGRSFPVEIKYAPAASRESLDPLPSSNNAAPYVCDVVKMATEIHAAEEDGAILAFLTSQAEVEWACEKFQSPSAIALPLHGKLFHEEQCRVFQNYPGKRKVVFATNLAETSITIPGVKYVVDSGLVKDSRFESSSGMNVLRVSKISQSSANQRAGRAGRTDPGKCYRLYSVSDYQSMDLHQEPEICKVHLGIAVLRILASGIKNVLEFDFIDAPSVDAINKAIRNLVQLGAVAWKHDAFVLTADGHYLVKLGMEPRLGKIILESLRYGLRKEGVVLAAAMANASNIFCRVGTYDEKLKSDCLKVRFCHHDGDLFTLLSVYREWESLRQENRNKWCWENRINAKTMRRCRDTVLELENCLKNELNIIIPTYWLWDPLVASVHDENMKKIILSSLADNVAMYSGYDRLGYEVVLSGEYFQLHPSCSLQVYNQKPHWVVFAELLSISSQYLVCVTAIDFDSLSTFIHPLFDVSKMESRKLQLRVIKGFGGVALKRFCGKSNSSLIALVSRMRAIYMDERIGIEINVGDNEIQLFASSKDIEKIYEYVNNALRYETKWLRNECLEKCLYHEVRAGASPPVALVGAGAEIKHLELGNRCLTVDVHLSNVNVVDDKEVLTFLEKSVSGICGYNKFTGIGQHGGDAERWGRVSFLTPEAARKALYFNGSELCGCVLKLSLSRSSVGGIRKSSFAAVKAKISWPRRYSKGYAIVRCERNDAQFIVDDCFNVLIGGRFVQCQTSTRDMNSVVIRGLDKETSEAEILEVLHKTTNRRILDVFLIRGDEANNHSVDAFEQAILKEIAPFMPSQGPLSNYCHVQVFAPEPKDSFMKAWITFDGKLHLEAAKALQHMQGKALAGCFSWQKMQCQQVFHSSASCSASVYAFIERQLNILLKSFKFRPGVCCNLERNENGSYRVKISANATKTVAELRRPLEQLMNGKKLLFSKDGIMLMKSLQQEMGTYILFDRQNLTVRIFGPEKKVALTEQKLIASLLALHDKEQTDIRLRGGAMPYDLMKKVVEKFGPDLHVLKETFPEAEFMLNTRRHVISFSGKKDLRLQVEQMIRDFVRSVGVNGSIKRYEDDNIACPICLCEVEDCYQLEACGHKFCQSCLVEQLESAMRGRDGFPVGCAHEGCGMHIWLTDLKSLLPCEKLEDLFRASLSAFVASSGGTYRFCPSPDCPSVYHVASGMVGDLFVCGACYAETCTRCHVEYHPFVSCEKYKELKEDPDMSLKEWCKGKEHVRNCPVCGYTIEKVDGCNHIECRCGKHICWVCLEVFMSGDDCYAHLRSVHPATL

Target ORF information:

RefSeq Version	XM_002323929.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0017s07680g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002323929.2

ORF Insert Sequence:

ATGATCACCA GCAATAACCA CCACCCCTCC ACACGCCACC ACCACCACTC CCAACACCGT 
CAGTCGCAGC ATTTCAACCG TCACCGGAAC TTCCGAAACC ACCAAAACCA AAGCCTTTTT 
GTAGTCAGGC TTTTGTCCAA CCACCGTAAT AATAACCGCA CGCAATCCCC ACTCGAAACC 
CTAATTTCCC AATGTAACCC TAAACCTGAC AAATCCGATA CAAACCCCAC GTCCGCCGTT 
GCTGCTCGTT TGTTTTTCCA CGACCAGAGC GATGCTATCG CCGCCGTCGT CTTCCTCTGG 
GAACGGAGGC TGGCTGGGGA TCATGTGTAT ACTCCGGTGA CGGATTTTGA TGTAAATGAA 
GGGGACTTGA ATGAGAGGAT TAGAGGGGAG GTAGTGAAGA AATTGGAGAG AAAGATTGAA 
AATTTGGCCG TGGAAATTGG TAAATTTACG AGCTTTTTCA AGCGGCCGAA AGGGGTTAGG 
GTTTATAGTG AGAATAAGGT GAAGAAGGAG GCGTTGAGGG TGGAAATGGA GGTGGTTGTG 
AAGAGGGTGG AGGAGTTTAG GAAGGGAATG AGGTGTTTGA TGGATTGCAT TGAAGGGAAG 
GAGATTGGTG ATTTGGGGGT TTTGAGGGTT TATGATGAGG GTAATGGGAG AAAGATGGGA 
ATTTTTTATT ATTGGAGCAG GATTCATTTT CTGATATTGA GAGAGTGTAG AAGGGTAGAA 
AATGGGTTGC CTGTTTATGG GTTTAGGAGT GAGTTTTTGA AGATGCTGCG TTCACAGCAG 
GAATATGCGT TGATTGTTTC TGATTTGATT TTGTGCTTCA AGCTTAGTGG TATATGCTTC 
TTGTATTTAA AGGAGTATTA TGATTTGAAA TTCGACTTTA ATCTTAGTTT GTGTGCTTAT 
GCATATTCTT GTATGATCTC TTATGAATGG TTGGTGATGG TCTTGATTGG AGAAACAGGT 
TCAGGGAAGA GTACGCAGTT GGCACAATTT ATTGCTGATT CAGGAGTTGC ATCAAGTGGT 
TCAATTTTGT GCACTCAACC CCGCAAAATT GCTGCTATAT CCCTGGGAAA AAGGGTCGGT 
GAAGAATGCA ATGGATGTTA TGAAGATAAT TCAATTATCT GCTACCCATC TTATTCATCT 
TCCCAGCAGT TTGGTTCGAA GGTAATATAT ATGACTGATC ACTGTCTACT GCAAAACCTC 
ATGAAAGATA AAAATTTGTT TGGAGTTTCG TGCATTATTG TAGATGAGGC TCATGAAAGA 
AGCTTGAATA CTGATCTTCT TTTGGGCTTG CTAAAAGAAT TACTTCAGGA AAGGCCTGAC 
TTACAGCTAA TTATAATGTC TGCAACAGTT GATGCAAGCA AATTATCCAG TTATTTCTTT 
GGCTGTGGAA CATTTCATGT GTTGGGGAGA AGCTTTCCTG TTGAAATCAA ATATGCTCCT 
GCTGCATCTA GAGAGTCCCT TGACCCCTTG CCGAGCTCCA ATAATGCAGC TCCATATGTT 
TGTGATGTTG TTAAGATGGC AACGGAAATT CATGCAGCAG AGGAGGATGG GGCAATACTT 
GCTTTTTTGA CTTCACAGGC AGAAGTAGAA TGGGCATGTG AAAAGTTTCA GTCTCCCTCT 
GCAATTGCGC TCCCATTACA TGGGAAGCTT TTCCATGAAG AGCAATGTCG TGTTTTTCAG 
AATTACCCAG GAAAGAGAAA AGTTGTTTTT GCTACAAATT TAGCGGAAAC ATCCATAACA 
ATTCCAGGGG TCAAGTACGT GGTTGATTCT GGTTTAGTTA AAGATAGCAG GTTTGAATCT 
TCATCTGGTA TGAATGTGCT CAGAGTTTCC AAGATCAGCC AAAGTTCTGC AAATCAACGA 
GCTGGCCGTG CTGGTAGAAC AGATCCTGGC AAGTGTTACC GGTTGTACTC GGTTTCTGAT 
TATCAGTCAA TGGATTTGCA TCAGGAACCT GAAATTTGTA AAGTTCATCT TGGCATTGCA 
GTTTTGAGGA TTCTTGCCTC TGGTATTAAG AATGTGCTAG AGTTTGATTT TATTGATGCT 
CCAAGTGTGG ATGCAATCAA CAAGGCAATC AGGAACCTTG TTCAGTTAGG TGCTGTTGCC 
TGGAAACATG ATGCTTTTGT ATTAACTGCT GATGGACACT ACTTAGTTAA ATTGGGTATG 
GAGCCTCGAC TTGGGAAAAT TATTCTTGAA AGTCTCCGTT ATGGTTTGCG CAAGGAGGGA 
GTTGTCCTTG CTGCAGCTAT GGCAAATGCC AGTAACATTT TTTGCAGAGT GGGTACTTAT 
GATGAGAAAC TGAAGTCTGA TTGCCTTAAG GTCCGGTTCT GCCATCACGA TGGAGATCTC 
TTCACTTTGC TCTCCGTCTA CAGGGAATGG GAGAGTTTAC GGCAAGAGAA TAGAAATAAA 
TGGTGTTGGG AAAATAGAAT CAATGCAAAA ACTATGCGAA GATGCAGAGA TACAGTATTG 
GAGTTGGAAA ACTGTTTGAA AAATGAACTC AATATCATTA TTCCAACTTA CTGGCTTTGG 
GATCCTCTTG TTGCCTCTGT GCATGATGAA AACATGAAGA AGATTATACT TTCTTCACTA 
GCAGATAATG TAGCTATGTA TTCTGGATAT GATCGCCTTG GTTATGAGGT TGTTTTAAGT 
GGTGAATACT TCCAACTTCA CCCTTCATGT TCATTACAAG TGTATAACCA GAAGCCACAT 
TGGGTTGTCT TTGCTGAACT CTTGTCCATA TCAAGTCAAT ATTTGGTTTG TGTTACAGCA 
ATTGACTTTG ACAGTTTATC TACCTTCATA CATCCTTTAT TTGATGTCTC AAAGATGGAA 
TCTAGAAAAC TGCAGTTGAG GGTGATAAAA GGATTTGGAG GTGTTGCTCT GAAACGGTTT 
TGTGGAAAGT CAAATAGTAG CTTGATAGCA CTTGTTTCTC GCATGCGTGC TATTTACATG 
GATGAACGCA TTGGAATTGA AATCAATGTG GGTGACAACG AGATTCAATT GTTTGCATCG 
TCCAAAGATA TTGAAAAGAT TTATGAATAT GTCAATAATG CGTTAAGATA TGAAACAAAG 
TGGTTAAGGA ATGAATGCTT GGAGAAGTGC TTATATCATG AGGTACGGGC TGGTGCTTCC 
CCCCCTGTTG CACTTGTTGG AGCTGGTGCT GAAATTAAGC ATCTGGAGTT GGGAAATAGA 
TGTTTAACAG TGGATGTGCA CCTTTCCAAT GTGAATGTGG TAGATGACAA GGAAGTTTTG 
ACATTCTTAG AGAAATCTGT ATCTGGAATT TGTGGTTATA ACAAATTCAC GGGCATTGGA 
CAGCATGGTG GTGATGCAGA AAGGTGGGGC AGGGTGTCAT TCCTTACTCC TGAAGCAGCT 
AGAAAAGCTC TATATTTTAA TGGATCCGAG TTATGTGGAT GTGTACTTAA GTTGTCCCTG 
TCTCGGTCAT CTGTTGGAGG TATTCGAAAG TCATCATTTG CTGCCGTAAA AGCAAAAATC 
AGTTGGCCAC GCCGGTATAG CAAAGGGTAT GCAATTGTCA GATGTGAAAG GAATGATGCC 
CAGTTCATTG TTGATGATTG CTTTAATGTT CTGATTGGAG GCAGGTTTGT TCAGTGTCAG 
ACCAGCACAA GAGACATGAA CTCTGTAGTT ATCAGAGGAC TGGATAAAGA AACTTCTGAA 
GCAGAAATTT TAGAAGTTCT GCACAAGACA ACAAATAGGA GAATTTTGGA TGTATTCCTA 
ATCAGAGGAG ATGAAGCAAA TAATCATTCT GTTGATGCTT TTGAGCAAGC AATTCTCAAG 
GAAATTGCTC CTTTCATGCC TAGCCAGGGC CCTTTGAGCA ATTACTGCCA TGTTCAGGTC 
TTTGCCCCTG AGCCAAAGGA TAGTTTCATG AAGGCTTGGA TCACTTTTGA TGGAAAATTA 
CATTTGGAGG CAGCTAAAGC TCTGCAACAT ATGCAAGGAA AAGCACTTGC TGGTTGTTTT 
TCATGGCAGA AGATGCAATG CCAGCAGGTG TTCCATAGCT CTGCATCATG TTCTGCTTCT 
GTTTATGCAT TCATTGAGAG GCAGCTGAAT ATTTTACTGA AAAGCTTCAA GTTTCGACCT 
GGTGTGTGTT GCAATTTAGA AAGGAATGAG AATGGCTCTT ATAGAGTGAA GATATCTGCT 
AATGCAACAA AGACAGTAGC TGAGTTGAGA AGGCCGCTGG AGCAACTGAT GAATGGAAAG 
AAGCTTCTCT TTTCCAAAGA TGGCATCATG CTTATGAAGT CACTGCAGCA AGAGATGGGA 
ACATATATTC TCTTTGATAG GCAGAACCTC ACTGTTAGGA TCTTTGGCCC CGAGAAGAAA 
GTTGCTCTTA CTGAACAGAA GTTGATAGCA TCTCTTCTAG CCCTCCATGA TAAGGAACAA 
ACAGATATCC GCCTTCGAGG TGGAGCAATG CCGTATGATC TTATGAAAAA GGTGGTGGAG 
AAGTTTGGTC CTGACTTGCA TGTGCTCAAA GAGACGTTCC CTGAGGCTGA ATTTATGCTT 
AATACAAGGC GACATGTTAT TTCTTTTTCT GGAAAAAAGG ATCTGAGACT CCAAGTGGAG 
CAGATGATTC GTGACTTTGT GCGGTCTGTC GGTGTTAATG GATCCATCAA ACGATATGAA 
GATGACAATA TTGCCTGCCC TATTTGCCTT TGTGAGGTTG AGGACTGTTA CCAGCTTGAG 
GCTTGTGGGC ATAAATTCTG CCAGTCATGC TTGGTTGAAC AGTTAGAGTC AGCAATGAGA 
GGACGTGATG GCTTCCCTGT AGGTTGTGCT CATGAAGGTT GTGGCATGCA CATTTGGCTT 
ACAGATCTGA AGTCTCTTTT ACCATGTGAG AAGTTAGAGG ATCTCTTCAG GGCCTCCTTG 
TCAGCCTTTG TGGCATCTAG TGGTGGAACT TACAGATTTT GCCCATCTCC TGATTGCCCT 
TCAGTATATC ACGTAGCAAG TGGGATGGTT GGTGATCTAT TTGTTTGTGG AGCATGCTAT 
GCCGAGACAT GTACAAGGTG CCATGTGGAG TATCATCCAT TCGTATCATG TGAAAAATAC 
AAGGAGCTCA AGGAGGATCC TGACATGTCC TTGAAGGAAT GGTGCAAGGG AAAAGAGCAT 
GTTAGAAACT GCCCAGTATG TGGATACACA ATTGAGAAGG TAGATGGCTG CAACCACATT 
GAGTGCAGAT GTGGGAAACA CATCTGCTGG GTCTGCTTGG AGGTCTTTAT GAGCGGTGAT 
GACTGCTATG CGCACTTGAG GTCTGTACAT CCTGCTACAT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7461259 ( XM_024588266.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024444034.1 Populus trichocarpa ATP-dependent RNA helicase DEAH11, chloroplastic (LOC7461259), mRNA.

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CloneID	OPa445966
Clone ID Related Accession (Same CDS sequence)	XM_024588266.1
Accession Version	XM_024588266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	ATP-dependent RNA helicase DEAH11, chloroplastic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037301.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACCA GCAATAACCA CCACCCCTCC ACACGCCACC ACCACCACTC CCAACACCGT 
CAGTCGCAGC ATTTCAACCG TCACCGGAAC TTCCGAAACC ACCAAAACCA AAGCCTTTTT 
GTAGTCAGGC TTTTGTCCAA CCACCGTAAT AATAACCGCA CGCAATCCCC ACTCGAAACC 
CTAATTTCCC AATGTAACCC TAAACCTGAC AAATCCGATA CAAACCCCAC GTCCGCCGTT 
GCTGCTCGTT TGTTTTTCCA CGACCAGAGC GATGCTATCG CCGCCGTCGT CTTCCTCTGG 
GAACGGAGGC TGGCTGGGGA TCATGTGTAT ACTCCGGTGA CGGATTTTGA TGTAAATGAA 
GGGGACTTGA ATGAGAGGAT TAGAGGTTTG TTTAAATTGT ATGCAGAGAG GGTGTTAGAA 
GGGGAGGTAG TGAAGAAATT GGAGAGAAAG ATTGAAAATT TGGCCGTGGA AATTGGTAAA 
TTTACGAGCT TTTTCAAGCG GCCGAAAGGG GTTAGGGTTT ATAGTGAGAA TAAGGTGAAG 
AAGGAGGCGT TGAGGGTGGA AATGGAGGTG GTTGTGAAGA GGGTGGAGGA GTTTAGGAAG 
GGAATGAGGT GTTTGATGGA TTGCATTGAA GGGAAGGAGA TTGGTGATTT GGGGGTTTTG 
AGGGTTTATG ATGAGGGTAA TGGGAGAAAG ATGGGAATTT TTTATTATTG GAGCAGGATT 
CATTTTCTGA TATTGAGAGA GTGTAGAAGG GTAGAAAATG GGTTGCCTGT TTATGGGTTT 
AGGAGTGAGT TTTTGAAGAT GCTGCGTTCA CAGCAGGTGA TGGTCTTGAT TGGAGAAACA 
GGTTCAGGGA AGAGTACGCA GTTGGCACAA TTTATTGCTG ATTCAGGAGT TGCATCAAGT 
GGTTCAATTT TGTGCACTCA ACCCCGCAAA ATTGCTGCTA TATCCCTGGG AAAAAGGGTC 
GGTGAAGAAT GCAATGGATG TTATGAAGAT AATTCAATTA TCTGCTACCC ATCTTATTCA 
TCTTCCCAGC AGTTTGGTTC GAAGGTAATA TATATGACTG ATCACTGTCT ACTGCAAAAC 
CTCATGAAAG ATAAAAATTT GTTTGGAGTT TCGTGCATTA TTGTAGATGA GGCTCATGAA 
AGAAGCTTGA ATACTGATCT TCTTTTGGGC TTGCTAAAAG AATTACTTCA GGAAAGGCCT 
GACTTACAGC TAATTATAAT GTCTGCAACA GTTGATGCAA GCAAATTATC CAGTTATTTC 
TTTGGCTGTG GAACATTTCA TGTGTTGGGG AGAAGCTTTC CTGTTGAAAT CAAATATGCT 
CCTGCTGCAT CTAGAGAGTC CCTTGACCCC TTGCCGAGCT CCAATAATGC AGCTCCATAT 
GTTTGTGATG TTGTTAAGAT GGCAACGGAA ATTCATGCAG CAGAGGAGGA TGGGGCAATA 
CTTGCTTTTT TGACTTCACA GGCAGAAGTA GAATGGGCAT GTGAAAAGTT TCAGTCTCCC 
TCTGCAATTG CGCTCCCATT ACATGGGAAG CTTTTCCATG AAGAGCAATG TCGTGTTTTT 
CAGAATTACC CAGGAAAGAG AAAAGTTGTT TTTGCTACAA ATTTAGCGGA AACATCCATA 
ACAATTCCAG GGGTCAAGTA CGTGGTTGAT TCTGGTTTAG TTAAAGATAG CAGGTTTGAA 
TCTTCATCTG GTATGAATGT GCTCAGAGTT TCCAAGATCA GCCAAAGTTC TGCAAATCAA 
CGAGCTGGCC GTGCTGGTAG AACAGATCCT GGCAAGTGTT ACCGGTTGTA CTCGGTTTCT 
GATTATCAGT CAATGGATTT GCATCAGGAA CCTGAAATTT GTAAAGTTCA TCTTGGCATT 
GCAGTTTTGA GGATTCTTGC CTCTGGTATT AAGAATGTGC TAGAGTTTGA TTTTATTGAT 
GCTCCAAGTG TGGATGCAAT CAACAAGGCA ATCAGGAACC TTGTTCAGTT AGGTGCTGTT 
GCCTGGAAAC ATGATGCTTT TGTATTAACT GCTGATGGAC GCTACTTAGT TAAATTGGGT 
ATGGAGCCTC GACTTGGGAA AATTATTCTT GAAAGTCTCC GTTATGGTTT GCGCAAGGAG 
GGAGTTGTCC TTGCTGCAGC TATGGCAAAT GCCAGTAACA TTTTTTGCAG AGTGGGTACT 
TATGATGAGA AACTGAAGTC TGATTGCCTT AAGGTCCGGT TCTGCCATCA CGATGGAGAT 
CTCTTCACTT TGCTCTCCGT CTACAGGGAA TGGGAGAGTT TACGGCAAGA GAATAGAAAT 
AAATGGTGTT GGGAAAATAG AATCAATGCA AAAACTATGC GAAGATGCAG AGATACAGTA 
TTGGAGTTGG AAAACTGTTT GAAAAATGAA CTCAATATCA TTATTCCAAC TTACTGGCTT 
TGGGATCCTC TTGTTGCCTC TGTGCATGAT GAAAACATGA AGAAGATTAT ACTTTCTTCA 
CTAGCAGATA ATGTAGCTAT GTATTCTGGA TATGATCGCC TTGGTTATGA GGTTGTTTTA 
AGTGGTGAAT ACTTCCAACT TCACCCTTCA TGTTCATTAC AAGTGTATAA CCAGAAGCCA 
CATTGGGTTG TCTTTGCTGA ACTCTTGTCC ATATCAAGTC AATATTTGGT TTGTGTTACA 
GCAATTGACT TTGACAGTTT ATCTACCTTC ATACATCCTT TATTTGATGT CTCAAAGATG 
GAATCTAGAA AACTGCAGTT GAGGGTGATA AAAGGATTTG GAGGTGTTGC TCTGAAACGG 
TTTTGTGGAA AGTCAAATAG TAGCTTGATA GCACTTGTTT CTCGCATGCG TGCTATTTAC 
ATGGATGAAC GCATTGGAAT TGAAATCAAT GTGGGTGACA ACGAGATTCA ATTGTTTGCA 
TCGTCCAAAG ATATTGAAAA GATTTATGAA TATGTCAATA ATGCGTTAAG ATATGAAACA 
AAGTGGTTAA GGAATGAATG CTTGGAGAAG TGCTTATATC ATGAGGTACG GGCTGGTGCT 
TCCCCCCCTG TTGCACTTGT TGGAGCTGGT GCTGAAATTA AGCATCTGGA GTTGGGAAAT 
AGATGTTTAA CAGTGGATGT GCACCTTTCC AATGTGAATG TGGTAGATGA CAAGGAAGTT 
TTGACATTCT TAGAGAAATC TGTATCTGGA ATTTGTGGTT ATAACAAATT CACGGGCATT 
GGACAGCATG GTGGTGATGC AGAAAGGTGG GGCAGGGTGT CATTCCTTAC TCCTGAAGCA 
GCTAGAAAAG CTCTATATTT TAATGGATCC GAGTTATGTG GATGTGTACT TAAGTTGTCC 
CTGTCTCGGT CATCTGTTGG AGGTATTCGA AAGTCATCAT TTGCTGCCGT AAAAGCAAAA 
ATCAGTTGGC CACGCCGGTA TAGCAAAGGG TATGCAATTG TCAGATGTGA AAGGAATGAT 
GCCCAGTTCA TTGTTGATGA TTGCTTTAAT GTTCTGATTG GAGGCAGGTT TGTTCAGTGT 
CAGACCAGCA CAAGAGACAT GAACTCTGTA GTTATCAGAG GACTGGATAA AGAAACTTCT 
GAAGCAGAAA TTTTAGAAGT TCTGCACAAG ACAACAAATA GGAGAATTTT GGATGTATTC 
CTAATCAGAG GAGATGAAGC AAATAATCAT TCTGTTGATG CTTTTGAGCA AGCAATTCTC 
AAGGAAATTG CTCCTTTCAT GCCTAGCCAG GGCCCTTTGA GCAATTACTG CCATGTTCAG 
GTCTTTGCCC CTGAGCCAAA GGATAGTTTC ATGAAGGCTT GGATCACTTT TGATGGAAAA 
TTACATTTGG AGGCAGCTAA AGCTCTGCAA CATATGCAAG GAAAAGCACT TGCTGGTTGT 
TTTTCATGGC AGAAGATGCA ATGCCAGCAG GTGTTCCATA GCTCTGCATC ATGTTCTGCT 
TCTGTTTATG CATTCATTGA GAGGCAGCTG AATATTTTAC TGAAAAGCTT CAAGTTTCGA 
CCTGGTGTGT GTTGCAATTT AGAAAGGAAT GAGAATGGCT CTTATAGAGT GAAGATATCT 
GCTAATGCAA CAAAGACAGT AGCTGAGTTG AGAAGGCCGC TGGAGCAACT GATGAATGGA 
AAGAAGGTAA ATCATTGTAG CCTCACCCCA TTGGTTCTGC AGCTTCTCTT TTCCAAAGAT 
GGCATCATGC TTATGAAGTC ACTGCAGCAA GAGATGGGAA CATATATTCT CTTTGATAGG 
CAGAACCTCA CTGTTAGGAT CTTTGGCCCC GAGAAGAAAG TTGCTCTTAC TGAACAGAAG 
TTGATAGCAT CTCTTCTAGC CCTCCATGAT AAGGAACAAA CAGATATCCG CCTTCGAGGT 
GGAGCAATGC CGTATGATCT TATGAAAAAG GTGGTGGAGA AGTTTGGTCC TGACTTGCAT 
GTGCTCAAAG AGACGTTCCC TGAGGCTGAA TTTATGCTTA ATACAAGGCG ACATGTTATT 
TCTTTTTCTG GAAAAAAGGA TCTGAGACTC CAAGTGGAGC AGATGATTCG TGACTTTGTG 
CGGTCTGTCG GTGTTAATGG ATCCATCAAA CGATATGAAG ATGACAATAT TGCCTGCCCT 
ATTTGCCTTT GTGAGGTTGA GGACTGTTAC CAGCTTGAGG CTTGTGGGCA TAAATTCTGC 
CAGTCATGCT TGGTTGAACA GTTAGAGTCA GCAATGAGAG GACGTGATGG CTTCCCTGTA 
GGTTGTGCTC ATGAAGGTTG TGGCATGCAC ATTTGGCTTA CAGATCTGAA GTCTCTTTTA 
CCATGTGAGA AGTTAGAGGA TCTCTTCAGG GCCTCCTTGT CAGCCTTTGT GGCATCTAGT 
GGTGGAACTT ACAGATTTTG CCCATCTCCT GATTGCCCTT CAGTATATCA CGTAGCAAGT 
GGGATGGTTG GTGATCTATT TGTTTGTGGA GCATGCTATG CCGAGACATG TACAAGGTGC 
CATGTGGAGT ATCATCCATT CGTATCATGT GAAAAATACA AGGAGCTCAA GGAGGATCCT 
GACATGTCCT TGAAGGAATG GTGCAAGGGA AAAGAGCATG TTAGAAACTG CCCAGTATGT 
GGATACACAA TTGAGAAGGT AGATGGCTGC AACCACATTG AGTGCAGATG TGGGAAACAC 
ATCTGCTGGG TCTGCTTGGA GGTCTTTATG AGCGGTGATG ACTGCTATGC GCACTTGAGG 
TCTGTACATC CTGCTACATT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024444034.1
CDS	169..5352
Translation	MITSNNHHPSTRHHHHSQHRQSQHFNRHRNFRNHQNQSLFVVRLLSNHRNNNRTQSPLETLISQCNPKPDKSDTNPTSAVAARLFFHDQSDAIAAVVFLWERRLAGDHVYTPVTDFDVNEGDLNERIRGLFKLYAERVLEGEVVKKLERKIENLAVEIGKFTSFFKRPKGVRVYSENKVKKEALRVEMEVVVKRVEEFRKGMRCLMDCIEGKEIGDLGVLRVYDEGNGRKMGIFYYWSRIHFLILRECRRVENGLPVYGFRSEFLKMLRSQQVMVLIGETGSGKSTQLAQFIADSGVASSGSILCTQPRKIAAISLGKRVGEECNGCYEDNSIICYPSYSSSQQFGSKVIYMTDHCLLQNLMKDKNLFGVSCIIVDEAHERSLNTDLLLGLLKELLQERPDLQLIIMSATVDASKLSSYFFGCGTFHVLGRSFPVEIKYAPAASRESLDPLPSSNNAAPYVCDVVKMATEIHAAEEDGAILAFLTSQAEVEWACEKFQSPSAIALPLHGKLFHEEQCRVFQNYPGKRKVVFATNLAETSITIPGVKYVVDSGLVKDSRFESSSGMNVLRVSKISQSSANQRAGRAGRTDPGKCYRLYSVSDYQSMDLHQEPEICKVHLGIAVLRILASGIKNVLEFDFIDAPSVDAINKAIRNLVQLGAVAWKHDAFVLTADGRYLVKLGMEPRLGKIILESLRYGLRKEGVVLAAAMANASNIFCRVGTYDEKLKSDCLKVRFCHHDGDLFTLLSVYREWESLRQENRNKWCWENRINAKTMRRCRDTVLELENCLKNELNIIIPTYWLWDPLVASVHDENMKKIILSSLADNVAMYSGYDRLGYEVVLSGEYFQLHPSCSLQVYNQKPHWVVFAELLSISSQYLVCVTAIDFDSLSTFIHPLFDVSKMESRKLQLRVIKGFGGVALKRFCGKSNSSLIALVSRMRAIYMDERIGIEINVGDNEIQLFASSKDIEKIYEYVNNALRYETKWLRNECLEKCLYHEVRAGASPPVALVGAGAEIKHLELGNRCLTVDVHLSNVNVVDDKEVLTFLEKSVSGICGYNKFTGIGQHGGDAERWGRVSFLTPEAARKALYFNGSELCGCVLKLSLSRSSVGGIRKSSFAAVKAKISWPRRYSKGYAIVRCERNDAQFIVDDCFNVLIGGRFVQCQTSTRDMNSVVIRGLDKETSEAEILEVLHKTTNRRILDVFLIRGDEANNHSVDAFEQAILKEIAPFMPSQGPLSNYCHVQVFAPEPKDSFMKAWITFDGKLHLEAAKALQHMQGKALAGCFSWQKMQCQQVFHSSASCSASVYAFIERQLNILLKSFKFRPGVCCNLERNENGSYRVKISANATKTVAELRRPLEQLMNGKKVNHCSLTPLVLQLLFSKDGIMLMKSLQQEMGTYILFDRQNLTVRIFGPEKKVALTEQKLIASLLALHDKEQTDIRLRGGAMPYDLMKKVVEKFGPDLHVLKETFPEAEFMLNTRRHVISFSGKKDLRLQVEQMIRDFVRSVGVNGSIKRYEDDNIACPICLCEVEDCYQLEACGHKFCQSCLVEQLESAMRGRDGFPVGCAHEGCGMHIWLTDLKSLLPCEKLEDLFRASLSAFVASSGGTYRFCPSPDCPSVYHVASGMVGDLFVCGACYAETCTRCHVEYHPFVSCEKYKELKEDPDMSLKEWCKGKEHVRNCPVCGYTIEKVDGCNHIECRCGKHICWVCLEVFMSGDDCYAHLRSVHPATL

Target ORF information:

RefSeq Version	XM_024588266.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa ATP-dependent RNA helicase DEAH11, chloroplastic (LOC7461259), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024588266.1

ORF Insert Sequence:

ATGATCACCA GCAATAACCA CCACCCCTCC ACACGCCACC ACCACCACTC CCAACACCGT 
CAGTCGCAGC ATTTCAACCG TCACCGGAAC TTCCGAAACC ACCAAAACCA AAGCCTTTTT 
GTAGTCAGGC TTTTGTCCAA CCACCGTAAT AATAACCGCA CGCAATCCCC ACTCGAAACC 
CTAATTTCCC AATGTAACCC TAAACCTGAC AAATCCGATA CAAACCCCAC GTCCGCCGTT 
GCTGCTCGTT TGTTTTTCCA CGACCAGAGC GATGCTATCG CCGCCGTCGT CTTCCTCTGG 
GAACGGAGGC TGGCTGGGGA TCATGTGTAT ACTCCGGTGA CGGATTTTGA TGTAAATGAA 
GGGGACTTGA ATGAGAGGAT TAGAGGTTTG TTTAAATTGT ATGCAGAGAG GGTGTTAGAA 
GGGGAGGTAG TGAAGAAATT GGAGAGAAAG ATTGAAAATT TGGCCGTGGA AATTGGTAAA 
TTTACGAGCT TTTTCAAGCG GCCGAAAGGG GTTAGGGTTT ATAGTGAGAA TAAGGTGAAG 
AAGGAGGCGT TGAGGGTGGA AATGGAGGTG GTTGTGAAGA GGGTGGAGGA GTTTAGGAAG 
GGAATGAGGT GTTTGATGGA TTGCATTGAA GGGAAGGAGA TTGGTGATTT GGGGGTTTTG 
AGGGTTTATG ATGAGGGTAA TGGGAGAAAG ATGGGAATTT TTTATTATTG GAGCAGGATT 
CATTTTCTGA TATTGAGAGA GTGTAGAAGG GTAGAAAATG GGTTGCCTGT TTATGGGTTT 
AGGAGTGAGT TTTTGAAGAT GCTGCGTTCA CAGCAGGTGA TGGTCTTGAT TGGAGAAACA 
GGTTCAGGGA AGAGTACGCA GTTGGCACAA TTTATTGCTG ATTCAGGAGT TGCATCAAGT 
GGTTCAATTT TGTGCACTCA ACCCCGCAAA ATTGCTGCTA TATCCCTGGG AAAAAGGGTC 
GGTGAAGAAT GCAATGGATG TTATGAAGAT AATTCAATTA TCTGCTACCC ATCTTATTCA 
TCTTCCCAGC AGTTTGGTTC GAAGGTAATA TATATGACTG ATCACTGTCT ACTGCAAAAC 
CTCATGAAAG ATAAAAATTT GTTTGGAGTT TCGTGCATTA TTGTAGATGA GGCTCATGAA 
AGAAGCTTGA ATACTGATCT TCTTTTGGGC TTGCTAAAAG AATTACTTCA GGAAAGGCCT 
GACTTACAGC TAATTATAAT GTCTGCAACA GTTGATGCAA GCAAATTATC CAGTTATTTC 
TTTGGCTGTG GAACATTTCA TGTGTTGGGG AGAAGCTTTC CTGTTGAAAT CAAATATGCT 
CCTGCTGCAT CTAGAGAGTC CCTTGACCCC TTGCCGAGCT CCAATAATGC AGCTCCATAT 
GTTTGTGATG TTGTTAAGAT GGCAACGGAA ATTCATGCAG CAGAGGAGGA TGGGGCAATA 
CTTGCTTTTT TGACTTCACA GGCAGAAGTA GAATGGGCAT GTGAAAAGTT TCAGTCTCCC 
TCTGCAATTG CGCTCCCATT ACATGGGAAG CTTTTCCATG AAGAGCAATG TCGTGTTTTT 
CAGAATTACC CAGGAAAGAG AAAAGTTGTT TTTGCTACAA ATTTAGCGGA AACATCCATA 
ACAATTCCAG GGGTCAAGTA CGTGGTTGAT TCTGGTTTAG TTAAAGATAG CAGGTTTGAA 
TCTTCATCTG GTATGAATGT GCTCAGAGTT TCCAAGATCA GCCAAAGTTC TGCAAATCAA 
CGAGCTGGCC GTGCTGGTAG AACAGATCCT GGCAAGTGTT ACCGGTTGTA CTCGGTTTCT 
GATTATCAGT CAATGGATTT GCATCAGGAA CCTGAAATTT GTAAAGTTCA TCTTGGCATT 
GCAGTTTTGA GGATTCTTGC CTCTGGTATT AAGAATGTGC TAGAGTTTGA TTTTATTGAT 
GCTCCAAGTG TGGATGCAAT CAACAAGGCA ATCAGGAACC TTGTTCAGTT AGGTGCTGTT 
GCCTGGAAAC ATGATGCTTT TGTATTAACT GCTGATGGAC GCTACTTAGT TAAATTGGGT 
ATGGAGCCTC GACTTGGGAA AATTATTCTT GAAAGTCTCC GTTATGGTTT GCGCAAGGAG 
GGAGTTGTCC TTGCTGCAGC TATGGCAAAT GCCAGTAACA TTTTTTGCAG AGTGGGTACT 
TATGATGAGA AACTGAAGTC TGATTGCCTT AAGGTCCGGT TCTGCCATCA CGATGGAGAT 
CTCTTCACTT TGCTCTCCGT CTACAGGGAA TGGGAGAGTT TACGGCAAGA GAATAGAAAT 
AAATGGTGTT GGGAAAATAG AATCAATGCA AAAACTATGC GAAGATGCAG AGATACAGTA 
TTGGAGTTGG AAAACTGTTT GAAAAATGAA CTCAATATCA TTATTCCAAC TTACTGGCTT 
TGGGATCCTC TTGTTGCCTC TGTGCATGAT GAAAACATGA AGAAGATTAT ACTTTCTTCA 
CTAGCAGATA ATGTAGCTAT GTATTCTGGA TATGATCGCC TTGGTTATGA GGTTGTTTTA 
AGTGGTGAAT ACTTCCAACT TCACCCTTCA TGTTCATTAC AAGTGTATAA CCAGAAGCCA 
CATTGGGTTG TCTTTGCTGA ACTCTTGTCC ATATCAAGTC AATATTTGGT TTGTGTTACA 
GCAATTGACT TTGACAGTTT ATCTACCTTC ATACATCCTT TATTTGATGT CTCAAAGATG 
GAATCTAGAA AACTGCAGTT GAGGGTGATA AAAGGATTTG GAGGTGTTGC TCTGAAACGG 
TTTTGTGGAA AGTCAAATAG TAGCTTGATA GCACTTGTTT CTCGCATGCG TGCTATTTAC 
ATGGATGAAC GCATTGGAAT TGAAATCAAT GTGGGTGACA ACGAGATTCA ATTGTTTGCA 
TCGTCCAAAG ATATTGAAAA GATTTATGAA TATGTCAATA ATGCGTTAAG ATATGAAACA 
AAGTGGTTAA GGAATGAATG CTTGGAGAAG TGCTTATATC ATGAGGTACG GGCTGGTGCT 
TCCCCCCCTG TTGCACTTGT TGGAGCTGGT GCTGAAATTA AGCATCTGGA GTTGGGAAAT 
AGATGTTTAA CAGTGGATGT GCACCTTTCC AATGTGAATG TGGTAGATGA CAAGGAAGTT 
TTGACATTCT TAGAGAAATC TGTATCTGGA ATTTGTGGTT ATAACAAATT CACGGGCATT 
GGACAGCATG GTGGTGATGC AGAAAGGTGG GGCAGGGTGT CATTCCTTAC TCCTGAAGCA 
GCTAGAAAAG CTCTATATTT TAATGGATCC GAGTTATGTG GATGTGTACT TAAGTTGTCC 
CTGTCTCGGT CATCTGTTGG AGGTATTCGA AAGTCATCAT TTGCTGCCGT AAAAGCAAAA 
ATCAGTTGGC CACGCCGGTA TAGCAAAGGG TATGCAATTG TCAGATGTGA AAGGAATGAT 
GCCCAGTTCA TTGTTGATGA TTGCTTTAAT GTTCTGATTG GAGGCAGGTT TGTTCAGTGT 
CAGACCAGCA CAAGAGACAT GAACTCTGTA GTTATCAGAG GACTGGATAA AGAAACTTCT 
GAAGCAGAAA TTTTAGAAGT TCTGCACAAG ACAACAAATA GGAGAATTTT GGATGTATTC 
CTAATCAGAG GAGATGAAGC AAATAATCAT TCTGTTGATG CTTTTGAGCA AGCAATTCTC 
AAGGAAATTG CTCCTTTCAT GCCTAGCCAG GGCCCTTTGA GCAATTACTG CCATGTTCAG 
GTCTTTGCCC CTGAGCCAAA GGATAGTTTC ATGAAGGCTT GGATCACTTT TGATGGAAAA 
TTACATTTGG AGGCAGCTAA AGCTCTGCAA CATATGCAAG GAAAAGCACT TGCTGGTTGT 
TTTTCATGGC AGAAGATGCA ATGCCAGCAG GTGTTCCATA GCTCTGCATC ATGTTCTGCT 
TCTGTTTATG CATTCATTGA GAGGCAGCTG AATATTTTAC TGAAAAGCTT CAAGTTTCGA 
CCTGGTGTGT GTTGCAATTT AGAAAGGAAT GAGAATGGCT CTTATAGAGT GAAGATATCT 
GCTAATGCAA CAAAGACAGT AGCTGAGTTG AGAAGGCCGC TGGAGCAACT GATGAATGGA 
AAGAAGGTAA ATCATTGTAG CCTCACCCCA TTGGTTCTGC AGCTTCTCTT TTCCAAAGAT 
GGCATCATGC TTATGAAGTC ACTGCAGCAA GAGATGGGAA CATATATTCT CTTTGATAGG 
CAGAACCTCA CTGTTAGGAT CTTTGGCCCC GAGAAGAAAG TTGCTCTTAC TGAACAGAAG 
TTGATAGCAT CTCTTCTAGC CCTCCATGAT AAGGAACAAA CAGATATCCG CCTTCGAGGT 
GGAGCAATGC CGTATGATCT TATGAAAAAG GTGGTGGAGA AGTTTGGTCC TGACTTGCAT 
GTGCTCAAAG AGACGTTCCC TGAGGCTGAA TTTATGCTTA ATACAAGGCG ACATGTTATT 
TCTTTTTCTG GAAAAAAGGA TCTGAGACTC CAAGTGGAGC AGATGATTCG TGACTTTGTG 
CGGTCTGTCG GTGTTAATGG ATCCATCAAA CGATATGAAG ATGACAATAT TGCCTGCCCT 
ATTTGCCTTT GTGAGGTTGA GGACTGTTAC CAGCTTGAGG CTTGTGGGCA TAAATTCTGC 
CAGTCATGCT TGGTTGAACA GTTAGAGTCA GCAATGAGAG GACGTGATGG CTTCCCTGTA 
GGTTGTGCTC ATGAAGGTTG TGGCATGCAC ATTTGGCTTA CAGATCTGAA GTCTCTTTTA 
CCATGTGAGA AGTTAGAGGA TCTCTTCAGG GCCTCCTTGT CAGCCTTTGT GGCATCTAGT 
GGTGGAACTT ACAGATTTTG CCCATCTCCT GATTGCCCTT CAGTATATCA CGTAGCAAGT 
GGGATGGTTG GTGATCTATT TGTTTGTGGA GCATGCTATG CCGAGACATG TACAAGGTGC 
CATGTGGAGT ATCATCCATT CGTATCATGT GAAAAATACA AGGAGCTCAA GGAGGATCCT 
GACATGTCCT TGAAGGAATG GTGCAAGGGA AAAGAGCATG TTAGAAACTG CCCAGTATGT 
GGATACACAA TTGAGAAGGT AGATGGCTGC AACCACATTG AGTGCAGATG TGGGAAACAC 
ATCTGCTGGG TCTGCTTGGA GGTCTTTATG AGCGGTGATG ACTGCTATGC GCACTTGAGG 
TCTGTACATC CTGCTACATT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC7461259 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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