LOC18107847 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC18107847
Entrez Gene ID	18107847
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006372141.1	XP_006372203.1	hypothetical protein
XM_024590233.1	XP_024446001.1	LOW QUALITY PROTEIN: DExH-box ATP-dependent RNA helicase DExH10

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC18107847 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC18107847 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa36493	XM_006372141.1 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0018s14280g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPa447748	XM_024590233.1 Latest version!	Populus trichocarpa DExH-box ATP-dependent RNA helicase DExH10 (LOC18107847), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC18107847 ( XM_006372141.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_006372203.1 Populus trichocarpa hypothetical protein (POPTR_0018s14280g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa36493
Clone ID Related Accession (Same CDS sequence)	XM_006372141.1
Accession Version	XM_006372141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008484). COMPLETENESS: incomplete on both ends.

ATGGAGGAAA CACTGACACC TACAAAGAGA AAAGAACCTG AGAAGGAGGA GGAGGAGGAG 
GAAGAGAAGA AGGAAGAGAA AGCAGAGATT GTTGGTGCTC AACAAAAGCA AGAATCTGCT 
TTGAAGAAGA GGAACTTGAC ACGGACCTGT GTTCATGAAG TTGTGGTCCC AAGTGGGTAT 
GTGGTGAATA AAGATGAAAC CCTTCATGGG ACTATGTCGA ATCCTTTGTA TAATGGGGAG 
ATGGCGAAAA GTTATAAATT TGAGCTTGAC CCTTTTCAGA AGGTGTCTGT AGCTTGTTTG 
GAAAGGAATG AGAGTGTTTT AGTTTCTGCT CATACGTCAG CAGGGAAAAC TGCGGTTGCT 
GAGTATGCGA TTGCAATGGC TTTTAGAGAA AAGCAAAGGG TTATTTATAC ATCTCCGTTG 
AAAGCTTTGA GTAATCAGAA GTACAGGGAG TTGCATCAGG AGTTTCAAGA TGTTGGATTG 
ATGACAGGAG ATGTTACTCT TTCTCCTAAT GCTAGCTGTT TGGTCATGAC TACGGAGATT 
CTTAGAGGAA TGCTTTATAG GGGTTCTGAG ATTCTGAAGG AAGTTGCTTG GGTTATTTTT 
GATGAGATTC ATTATATGAA GGATCGTGAA AGAGGGGTTG TTTGGGAAGA GAGTATTATA 
TTTATGCCGC CAGAGATTAA GATGGTTTTT CTTTCCGCGA CAATGTCGAA TGCTACTGAG 
TTTGCTGAGT GGATTTGTCA TTTGCATAAG CAGCCATGCC ATGTGGTTTA CACAGATTTT 
CGACCAACTC CTTTGCAGCA TTATGTCTTT CCTGTGGGTG GTGCTGGGTT ATATCTTGTG 
GTTGATGAGA GCGAGCAGTT TAGGGAGGAC AATTTTATGA AGCTGCAGGA TACTTTCTCG 
AAGCAGAAAA TTGGTGAGGG GAATAAGAGT GCAAATGGTA AGGCTAGTGG AAGAATTGCA 
AAGGGTGGGA ATGCTTCTGG GGGTTCTGAC ATATACAAAA TTGTCAAGAT GATCATGGAA 
CGGAAATTTC AACCAGTTAT AGTTTTTAGC TTCAGTAGGA GAGAGGTTGA ACAACATGCC 
ATGTCTATGT CCAAGCTCGA TTTTAATACA CAAGAAGAAA AGGAAATTGT GGAGCAGGTA 
TTTAACAACG CAATACTCTG CTTGAATGAG GAAGACAGGA ATTTGCCTGC TATTGAGTTG 
ATGTTGCCAC TACTTCAGAG AGGCATTGCT GTCCATCATT CTGGTCTTCT CCCTGTTATT 
AAGGAACTAG TAGAGCTTCT TTTCCAGGAA GGCCTTGTTA AGGCTTTATT TGCCACAGAG 
ACATTTGCAA TGGGTTTAAA CATGCCTGCA AAGACTGTTG TTTTCACAGC TGTTAAGAAG 
TGGGATGGTG ACAGTCATCG TTACATTGGA TCTGGTGAGT ATATCCAGAT GAGTGGAAGG 
GCTGGACATC GTGGCAAAGA TGAGCGGGGT ATTTGTATCA TAATGATTGA TGAGCGGATG 
GAGATGAATA CACTCAAAGA CATGGTTTTG GGTAAACCAG CCCCTCTTGT TAGTACTTTC 
AGACTGAGCT ACTATTCAAT CTTGAATTTA ATGAGCCGTG CTGAAGGCCA GTTCACTGCT 
GAACACGTAA TAAGAAATTC ATTTCATCAA TTCCAATATG AGAAGGCTTT ACCAGACATA 
GGGAAAAAGG TTTCAAAACT GGAAGAGGAA GCTGCCATGC TTGATGCTTC GGGAGAGAAG 
ATGATGACAG AAATAACGAG GCCTGAAAGA ATTCTTTATT ATCTATGCAC TGGTCGGTTT 
GGGAGCATTG CCATCAAAAG GCGCGGTGGT TATATAGTGG ATATTTTACT TCATTGCTCT 
CCTGGACCAA GTGAGAGTGG CTCTCGGCCA AGACCATGCC CAACTCGGCC TGGAGAAAAG 
GGTTGGATGC ATGTGGTTCC TGTTCAGTTG CCCCTGATTT GTGCTCTAAG TAAGGTAAGG 
ATATCCATCC CTGCCGACCT TCGGCCTTTG GAAGCAAGAC AAAGTATACT ACTTGCAGTG 
CAGGAGTTAG GGAATCGGTT TCCTGAAGGC CTCCCTAAGC TTAACCCTGT GAAGGACATG 
AAAATCGAGG ATCCTGAAAT TGTCGAGTTG GTTAATCAAA TTGAGGAATT GGAACGAAAG 
CTACATGCAC ATCCACTGCA CAAGTCTCAA GATGTGAATC AGATGAAAAG TTTTCATAGA 
AAAGCTGAGG TGAATCATGA AATTCAACAA CTCAAATCGA AAATGCGTGA TTCCCAGCTA 
AAGGGAAGAG CAGCCTGCTT AATAGACACA GGAGATGAAT TACTTGTTAC AGAACTGATG 
TTCATGGTGA GTAGTTTCAA CGATCTTGAT CATCACCAAG TGGCTGCCCT TGCTAGTTGC 
TTCAATCCAG TAGACAAGTC AAGTGAGCAG ATACAGTTGA GAACAGAACT TGCTAAACCA 
TTACAGCAAC TTCAAGAGAG TGCACGAAAA ATAGCAGAGA TACAATATGA ATGCAAATTG 
GACATAAATG TCGATGAATA TGTAGAATCA ACAGTCAGAC CATTCTTGAT GGATGTGATT 
TACTGTTGGT CAAAGGGAGC TAGTTTTTCA GAGGTGATGC AAATGACTGA TATCTTTGAG 
GGTAGCGTTA TCAGAAGTTC AAGAAGACTA GATGAATTTT TGAACCAGTT GCGTGCTGCT 
GCCCGAGCTG TGGGTGAAGT CAGTTTGGAG AGTAAATTTG CAGCTGCCAG CGAAAGCCTT 
CGTCGAGGTA TCATGTTTGC AAATTCTCTT TACCTGCAAG TTGTCTTTTT GCAGGCATTT 
GTTGTGTGGG TGGAATGGCT TGCAATGTGC AAGCTGAACT GCCCTCCTTT TTCACTCACA 
TCAATCTTCT CCATCCCAGG AGGAGCTATC ATCTCAAAAT TCGACACCAG TCTTGCAACA 
ATCAGTCCTA GGATTGGCAT TGCAAGTATG ATCCCTGGGC AGGAACGTCT ACCCACGCCA 
AATGGTAGGA ACCGAAAATC AACCTTTCCA CCAACGATGG CCTCTGTGTC ACGTTCTTCT 
TCCAAGAATC GCTCTGGCCG GAATTCTGAT GGTTTCTCCC ACCATTCAGG GTTGTTAGCT 
AGCCACCATG CATTGACGAC CACCTTTGAC TCTTTAGGAA TGGTAAACCC TCCTAGCTTT 
GCTTCTTCAA GGTTCATATG TGGCACTAAC AAGGGAATTG GGGTGTGTAG CCTTAATGTC 
TCTTTTATTG TGGCTTGCAA GTATGGTAGC TCATGCAGGT TAGATTCTGT AACTGGACTT 
CCCTTTAGGA CGGCTCTGAT TTCATCTCTG ATCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006372203.1
CDS	1..3342
Translation	MEETLTPTKRKEPEKEEEEEEEKKEEKAEIVGAQQKQESALKKRNLTRTCVHEVVVPSGYVVNKDETLHGTMSNPLYNGEMAKSYKFELDPFQKVSVACLERNESVLVSAHTSAGKTAVAEYAIAMAFREKQRVIYTSPLKALSNQKYRELHQEFQDVGLMTGDVTLSPNASCLVMTTEILRGMLYRGSEILKEVAWVIFDEIHYMKDRERGVVWEESIIFMPPEIKMVFLSATMSNATEFAEWICHLHKQPCHVVYTDFRPTPLQHYVFPVGGAGLYLVVDESEQFREDNFMKLQDTFSKQKIGEGNKSANGKASGRIAKGGNASGGSDIYKIVKMIMERKFQPVIVFSFSRREVEQHAMSMSKLDFNTQEEKEIVEQVFNNAILCLNEEDRNLPAIELMLPLLQRGIAVHHSGLLPVIKELVELLFQEGLVKALFATETFAMGLNMPAKTVVFTAVKKWDGDSHRYIGSGEYIQMSGRAGHRGKDERGICIIMIDERMEMNTLKDMVLGKPAPLVSTFRLSYYSILNLMSRAEGQFTAEHVIRNSFHQFQYEKALPDIGKKVSKLEEEAAMLDASGEKMMTEITRPERILYYLCTGRFGSIAIKRRGGYIVDILLHCSPGPSESGSRPRPCPTRPGEKGWMHVVPVQLPLICALSKVRISIPADLRPLEARQSILLAVQELGNRFPEGLPKLNPVKDMKIEDPEIVELVNQIEELERKLHAHPLHKSQDVNQMKSFHRKAEVNHEIQQLKSKMRDSQLKGRAACLIDTGDELLVTELMFMVSSFNDLDHHQVAALASCFNPVDKSSEQIQLRTELAKPLQQLQESARKIAEIQYECKLDINVDEYVESTVRPFLMDVIYCWSKGASFSEVMQMTDIFEGSVIRSSRRLDEFLNQLRAAARAVGEVSLESKFAAASESLRRGIMFANSLYLQVVFLQAFVVWVEWLAMCKLNCPPFSLTSIFSIPGGAIISKFDTSLATISPRIGIASMIPGQERLPTPNGRNRKSTFPPTMASVSRSSSKNRSGRNSDGFSHHSGLLASHHALTTTFDSLGMVNPPSFASSRFICGTNKGIGVCSLNVSFIVACKYGSSCRLDSVTGLPFRTALISSLIFL

Target ORF information:

RefSeq Version	XM_006372141.1
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0018s14280g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006372141.1

ORF Insert Sequence:

ATGGAGGAAA CACTGACACC TACAAAGAGA AAAGAACCTG AGAAGGAGGA GGAGGAGGAG 
GAAGAGAAGA AGGAAGAGAA AGCAGAGATT GTTGGTGCTC AACAAAAGCA AGAATCTGCT 
TTGAAGAAGA GGAACTTGAC ACGGACCTGT GTTCATGAAG TTGTGGTCCC AAGTGGGTAT 
GTGGTGAATA AAGATGAAAC CCTTCATGGG ACTATGTCGA ATCCTTTGTA TAATGGGGAG 
ATGGCGAAAA GTTATAAATT TGAGCTTGAC CCTTTTCAGA AGGTGTCTGT AGCTTGTTTG 
GAAAGGAATG AGAGTGTTTT AGTTTCTGCT CATACGTCAG CAGGGAAAAC TGCGGTTGCT 
GAGTATGCGA TTGCAATGGC TTTTAGAGAA AAGCAAAGGG TTATTTATAC ATCTCCGTTG 
AAAGCTTTGA GTAATCAGAA GTACAGGGAG TTGCATCAGG AGTTTCAAGA TGTTGGATTG 
ATGACAGGAG ATGTTACTCT TTCTCCTAAT GCTAGCTGTT TGGTCATGAC TACGGAGATT 
CTTAGAGGAA TGCTTTATAG GGGTTCTGAG ATTCTGAAGG AAGTTGCTTG GGTTATTTTT 
GATGAGATTC ATTATATGAA GGATCGTGAA AGAGGGGTTG TTTGGGAAGA GAGTATTATA 
TTTATGCCGC CAGAGATTAA GATGGTTTTT CTTTCCGCGA CAATGTCGAA TGCTACTGAG 
TTTGCTGAGT GGATTTGTCA TTTGCATAAG CAGCCATGCC ATGTGGTTTA CACAGATTTT 
CGACCAACTC CTTTGCAGCA TTATGTCTTT CCTGTGGGTG GTGCTGGGTT ATATCTTGTG 
GTTGATGAGA GCGAGCAGTT TAGGGAGGAC AATTTTATGA AGCTGCAGGA TACTTTCTCG 
AAGCAGAAAA TTGGTGAGGG GAATAAGAGT GCAAATGGTA AGGCTAGTGG AAGAATTGCA 
AAGGGTGGGA ATGCTTCTGG GGGTTCTGAC ATATACAAAA TTGTCAAGAT GATCATGGAA 
CGGAAATTTC AACCAGTTAT AGTTTTTAGC TTCAGTAGGA GAGAGGTTGA ACAACATGCC 
ATGTCTATGT CCAAGCTCGA TTTTAATACA CAAGAAGAAA AGGAAATTGT GGAGCAGGTA 
TTTAACAACG CAATACTCTG CTTGAATGAG GAAGACAGGA ATTTGCCTGC TATTGAGTTG 
ATGTTGCCAC TACTTCAGAG AGGCATTGCT GTCCATCATT CTGGTCTTCT CCCTGTTATT 
AAGGAACTAG TAGAGCTTCT TTTCCAGGAA GGCCTTGTTA AGGCTTTATT TGCCACAGAG 
ACATTTGCAA TGGGTTTAAA CATGCCTGCA AAGACTGTTG TTTTCACAGC TGTTAAGAAG 
TGGGATGGTG ACAGTCATCG TTACATTGGA TCTGGTGAGT ATATCCAGAT GAGTGGAAGG 
GCTGGACATC GTGGCAAAGA TGAGCGGGGT ATTTGTATCA TAATGATTGA TGAGCGGATG 
GAGATGAATA CACTCAAAGA CATGGTTTTG GGTAAACCAG CCCCTCTTGT TAGTACTTTC 
AGACTGAGCT ACTATTCAAT CTTGAATTTA ATGAGCCGTG CTGAAGGCCA GTTCACTGCT 
GAACACGTAA TAAGAAATTC ATTTCATCAA TTCCAATATG AGAAGGCTTT ACCAGACATA 
GGGAAAAAGG TTTCAAAACT GGAAGAGGAA GCTGCCATGC TTGATGCTTC GGGAGAGAAG 
ATGATGACAG AAATAACGAG GCCTGAAAGA ATTCTTTATT ATCTATGCAC TGGTCGGTTT 
GGGAGCATTG CCATCAAAAG GCGCGGTGGT TATATAGTGG ATATTTTACT TCATTGCTCT 
CCTGGACCAA GTGAGAGTGG CTCTCGGCCA AGACCATGCC CAACTCGGCC TGGAGAAAAG 
GGTTGGATGC ATGTGGTTCC TGTTCAGTTG CCCCTGATTT GTGCTCTAAG TAAGGTAAGG 
ATATCCATCC CTGCCGACCT TCGGCCTTTG GAAGCAAGAC AAAGTATACT ACTTGCAGTG 
CAGGAGTTAG GGAATCGGTT TCCTGAAGGC CTCCCTAAGC TTAACCCTGT GAAGGACATG 
AAAATCGAGG ATCCTGAAAT TGTCGAGTTG GTTAATCAAA TTGAGGAATT GGAACGAAAG 
CTACATGCAC ATCCACTGCA CAAGTCTCAA GATGTGAATC AGATGAAAAG TTTTCATAGA 
AAAGCTGAGG TGAATCATGA AATTCAACAA CTCAAATCGA AAATGCGTGA TTCCCAGCTA 
AAGGGAAGAG CAGCCTGCTT AATAGACACA GGAGATGAAT TACTTGTTAC AGAACTGATG 
TTCATGGTGA GTAGTTTCAA CGATCTTGAT CATCACCAAG TGGCTGCCCT TGCTAGTTGC 
TTCAATCCAG TAGACAAGTC AAGTGAGCAG ATACAGTTGA GAACAGAACT TGCTAAACCA 
TTACAGCAAC TTCAAGAGAG TGCACGAAAA ATAGCAGAGA TACAATATGA ATGCAAATTG 
GACATAAATG TCGATGAATA TGTAGAATCA ACAGTCAGAC CATTCTTGAT GGATGTGATT 
TACTGTTGGT CAAAGGGAGC TAGTTTTTCA GAGGTGATGC AAATGACTGA TATCTTTGAG 
GGTAGCGTTA TCAGAAGTTC AAGAAGACTA GATGAATTTT TGAACCAGTT GCGTGCTGCT 
GCCCGAGCTG TGGGTGAAGT CAGTTTGGAG AGTAAATTTG CAGCTGCCAG CGAAAGCCTT 
CGTCGAGGTA TCATGTTTGC AAATTCTCTT TACCTGCAAG TTGTCTTTTT GCAGGCATTT 
GTTGTGTGGG TGGAATGGCT TGCAATGTGC AAGCTGAACT GCCCTCCTTT TTCACTCACA 
TCAATCTTCT CCATCCCAGG AGGAGCTATC ATCTCAAAAT TCGACACCAG TCTTGCAACA 
ATCAGTCCTA GGATTGGCAT TGCAAGTATG ATCCCTGGGC AGGAACGTCT ACCCACGCCA 
AATGGTAGGA ACCGAAAATC AACCTTTCCA CCAACGATGG CCTCTGTGTC ACGTTCTTCT 
TCCAAGAATC GCTCTGGCCG GAATTCTGAT GGTTTCTCCC ACCATTCAGG GTTGTTAGCT 
AGCCACCATG CATTGACGAC CACCTTTGAC TCTTTAGGAA TGGTAAACCC TCCTAGCTTT 
GCTTCTTCAA GGTTCATATG TGGCACTAAC AAGGGAATTG GGGTGTGTAG CCTTAATGTC 
TCTTTTATTG TGGCTTGCAA GTATGGTAGC TCATGCAGGT TAGATTCTGT AACTGGACTT 
CCCTTTAGGA CGGCTCTGAT TTCATCTCTG ATCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107847 ( XM_024590233.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024446001.1 Populus trichocarpa DExH-box ATP-dependent RNA helicase DExH10 (LOC18107847), mRNA.

Price & Availability↑

CloneID	OPa447748
Clone ID Related Accession (Same CDS sequence)	XM_024590233.1
Accession Version	XM_024590233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	LOW QUALITY PROTEIN: DExH-box ATP-dependent RNA helicase DExH10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037302.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 3 indels internal stop codons :: corrected 1 genomic stop codons ##RefSeq-Attributes-END##

ATGGAGGAAA CACTGACACC TACAAAGAGA AAAGAACCTG AGAAGGAGGA GGAGGAGGAG 
GAAGAGAAGA AGGAAGAGAA AGCAGAGATT GTTGGTGCTC AACAAAAGCA AGAATCTGCT 
TTGAAGAAGA GGAACTTGAC ACGGACCTGT GTTCATGAAG TTGTGGTCCC AAGTGGGTAT 
GTGGTGAATA AAGATGAAAC CCTTCATGGG ACTATGTCGA ATCCTTTGTA TAATGGGGAG 
ATGGCGAAAA GTTATAAATT TGAGCTTGAC CCTTTTCAGA AGGTGTCTGT AGCTTGTTTG 
GAAAGGAATG AGAGTGTTTT AGTTTCTGCT CATACGTCAG CAGGGAAAAC TGCGGTTGCT 
GAGTATGCGA TTGCAATGGC TTTTAGAGAA AAGCAAAGGG TTATTTATAC ATCTCCGTTG 
AAAGCTTTGA GTAATCAGAA GTACAGGGAG TTGCATCAGG AGTTTCAAGA TGTTGGATTG 
ATGACAGGAG ATGTTACTCT TTCTCCTAAT GCTAGCTGTT TGGTCATGAC TACGGAGATT 
CTTAGAGGAA TGCTTTATAG GGGTTCTGAG ATTCTGAAGG AAGTTGCTTG GGTTATTTTT 
GATGAGATTC ATTATATGAA GGATCGTGAA AGAGGGGTTG TTTGGGAAGA GAGTATTATA 
TTTATGCCGC CAGAGATTAA GATGGTTTTT CTTTCCGCGA CAATGTCGAA TGCTACTGAG 
TTTGCTGAGT GGATTTGTCA TTTGCATAAG CAGCCATGCC ATGTGGTTTA CACAGATTTT 
CGACCAACTC CTTTGCAGCA TTATGTCTTT CCTGTGGGTG GTGCTGGGTT ATATCTTGTG 
GTTGATGAGA GCGAGCAGTT TAGGGAGGAC AATTTTATGA AGCTGCAGGA TACTTTCTCG 
AAGCAGAAAA TTGGTGAGGG GAATAAGAGT GCAAATGGTA AGGCTAGTGG AAGAATTGCA 
AAGGGTGGGA ATGCTTCTGG GGGTTCTGAC ATATACAAAA TTGTCAAGAT GATCATGGAA 
CGGAAATTTC AACCAGTTAT AGTTTTTAGC TTCAGTAGGA GAGAGGTTGA ACAACATGCC 
ATGTCTATGT CCAAGCTCGA TTTTAATACA CAAGAAGAAA AGGAAATTGT GGAGCAGGTA 
TTTAACAACG CAATACTCTG CTTGAATGAG GAAGACAGGA ATTTGCCTGC TATTGAGTTG 
ATGTTGCCAC TACTTCAGAG AGGCATTGCT GTCCATCATT CTGGTCTTCT CCCTGTTATT 
AAGGAACTAG TAGAGCTTCT TTTCCAGGAA GGCCTTGTTA AGGCTTTATT TGCCACAGAG 
ACATTTGCAA TGGGTTTAAA CATGCCTGCA AAGACTGTTG TTTTCACAGC TGTTAAGAAG 
TGGGATGGTG ACAGTCATCG TTACATTGGA TCTGGTGAGT ATATCCAGAT GAGTGGAAGG 
GCTGGACATC GTGGCAAAGA TGAGCGGGGT ATTTGTATCA TAATGATTGA TGAGCGGATG 
GAGATGAATA CACTCAAAGA CATGGTTTTG GGTAAACCAG CCCCTCTTGT TAGTACTTTC 
AGACTGAGCT ACTATTCAAT CTTGAATTTA ATGAGCCGTG CTGAAGGCCA GTTCACTGCT 
GAACACGTAA TAAGAAATTC ATTTCATCAA TTCCAATATG AGAAGGCTTT ACCAGACATA 
GGGAAAAAGG TTTCAAAACT GGAAGAGGAA GCTGCCATGC TTGATGCTTC GGGAGAGGCT 
GAGGTTGCTG AGTATCATAA TCTAAAACTT GAGATGACCC AANCTTCAAA GAAGATGATG 
ACAGAAATAA CGAGGCCTGA AAGAATTCTT TATTATCTAT GCACTGGTCG GTTGATCAAG 
GTAAGAGAAG GAGGANATTG GGGTTGGGGA GTTGTAGTGA ATGTTGTTAA AAAGCCCACT 
GCAGGTTTGG GAGCATTGCC ATCAAAAGGC GGTGGTTATA TAGTGGATAT TTTACTTCAT 
TGCTCTCCTG GACCAAGTGA GAGTGGCTCT CGGCCAAGAC CATGCCCGCC TCGGCCTGGA 
GAAAAGGGTT GGATGCATGT GGTTCCTGTT CAGTTGCCCC TGATTTGTGC TCTAAGTAAG 
GTAAGGATAT CCATCCCTGC CGACCTTCGG CCTTTGGAAG CAAGACAAAG TATACTACTT 
GCAGTGCAGG AGTTAGGGAA TCGGTTTCCT GAAGGCCTCC CTAAGCTTAA CCCTGTGAAG 
GACATGAAAA TCGAGGATCC TGAAATTGTC GAGTTGGTTA ATCAAATTGA GGAATTGGAA 
CGAAAGCTAC ATGCACATCC ACTGCACAAG TCTCAAGATG TGAATCAGAT GAAAAGTTTT 
CATAGAAAAG CTGAGGTGAA TCATGAAATT CAACAACTCA AATCGAAAAT GCGTGATTCC 
CAGCTTCAAA AATTTCGTGA AGAACTCAAG AACCGATCGT GAGTCTTGAA AAGACTTGGA 
TATATAGATG CTGATGGTGT TGTCCAGCTA AAGGGAAGAG CAGCCTGCTT AATAGACACA 
GGAGATGAAT TACTTGTTAC AGAACTGATG TTCATGGTGA GTAGTTTCAA CGATCTTGAT 
CATCACCAAG TGGCTGCCCT TGCTAGTTGC TTCAATCCAG TAGACAAGTC AAGTGAGCAG 
ATACAGTTGA GAACAGAACT TGCTAAACCA TTACAGCAAC TTCAAGAGAG TGCACGAAAA 
ATAGCAGAGA TACAATATGA ATGCAAATTG GACATAAATG TCGATGAATA TGTAGAATCA 
ACAGTCAGAC CATTCTTGAT GGATGTGATT TACTGTTGGT CAAAGGGAGC TAGTTTTTCA 
GAGGTGATGC AAATGACTGA TATCTTTGAG GGTAGCGTTA TCAGAAGTTC GAGAAGACTA 
GATGAATTTT TGAACCAGTT GCGTGCTGCT GCCCGAGCTG TGGGTGAAGT CAGTTTGGAG 
AGTAAATTTG CAGCTGCCAG CGAAAGCCTT CGTCGAGGTA TCATGTTTGC AAATTCTCTT 
TACCTGCAAG TTGTCTTTTT GCAGGCATTT GTTGTGTGGG GTGCCCATGA GCACAAAAGT 
TCTTACCTTG CAACAATTAT ACCGTGGTGG CCACTTAAGA TGTGCTATAT ACTGGCAACT 
ATAGATTCCA TCTCCTTTTC TCCTTCACTT TTGAGTTTCT TTCATGCTGT TGATCTTATA 
TATTTGACAG TTGATTTCAT TGTATACACA CAAAGTTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024446001.1
CDS	450..3671
Translation	MEETLTPTKRKEPEKEEEEEEEKKEEKAEIVGAQQKQESALKKRNLTRTCVHEVVVPSGYVVNKDETLHGTMSNPLYNGEMAKSYKFELDPFQKVSVACLERNESVLVSAHTSAGKTAVAEYAIAMAFREKQRVIYTSPLKALSNQKYRELHQEFQDVGLMTGDVTLSPNASCLVMTTEILRGMLYRGSEILKEVAWVIFDEIHYMKDRERGVVWEESIIFMPPEIKMVFLSATMSNATEFAEWICHLHKQPCHVVYTDFRPTPLQHYVFPVGGAGLYLVVDESEQFREDNFMKLQDTFSKQKIGEGNKSANGKASGRIAKGGNASGGSDIYKIVKMIMERKFQPVIVFSFSRREVEQHAMSMSKLDFNTQEEKEIVEQVFNNAILCLNEEDRNLPAIELMLPLLQRGIAVHHSGLLPVIKELVELLFQEGLVKALFATETFAMGLNMPAKTVVFTAVKKWDGDSHRYIGSGEYIQMSGRAGHRGKDERGICIIMIDERMEMNTLKDMVLGKPAPLVSTFRLSYYSILNLMSRAEGQFTAEHVIRNSFHQFQYEKALPDIGKKVSKLEEEAAMLDASGEAEVAEYHNLKLEMTQnullSKKMMTEITRPERILYYLCTGRLIKVREGGnullWGWGVVVNVVKKPTAGLGALPSKGGGYIVDILLHCSPGPSESGSRPRPCPPRPGEKGWMHVVPVQLPLICALSKVRISIPADLRPLEARQSILLAVQELGNRFPEGLPKLNPVKDMKIEDPEIVELVNQIEELERKLHAHPLHKSQDVNQMKSFHRKAEVNHEIQQLKSKMRDSQLQKFREELKNRS*VLKRLGYIDADGVVQLKGRAACLIDTGDELLVTELMFMVSSFNDLDHHQVAALASCFNPVDKSSEQIQLRTELAKPLQQLQESARKIAEIQYECKLDINVDEYVESTVRPFLMDVIYCWSKGASFSEVMQMTDIFEGSVIRSSRRLDEFLNQLRAAARAVGEVSLESKFAAASESLRRGIMFANSLYLQVVFLQAFVVWGAHEHKSSYLATIIPWWPLKMCYILATIDSISFSPSLLSFFHAVDLIYLTVDFIVYTQSS

Target ORF information:

RefSeq Version	XM_024590233.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa DExH-box ATP-dependent RNA helicase DExH10 (LOC18107847), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024590233.1

ORF Insert Sequence:

ATGGAGGAAA CACTGACACC TACAAAGAGA AAAGAACCTG AGAAGGAGGA GGAGGAGGAG 
GAAGAGAAGA AGGAAGAGAA AGCAGAGATT GTTGGTGCTC AACAAAAGCA AGAATCTGCT 
TTGAAGAAGA GGAACTTGAC ACGGACCTGT GTTCATGAAG TTGTGGTCCC AAGTGGGTAT 
GTGGTGAATA AAGATGAAAC CCTTCATGGG ACTATGTCGA ATCCTTTGTA TAATGGGGAG 
ATGGCGAAAA GTTATAAATT TGAGCTTGAC CCTTTTCAGA AGGTGTCTGT AGCTTGTTTG 
GAAAGGAATG AGAGTGTTTT AGTTTCTGCT CATACGTCAG CAGGGAAAAC TGCGGTTGCT 
GAGTATGCGA TTGCAATGGC TTTTAGAGAA AAGCAAAGGG TTATTTATAC ATCTCCGTTG 
AAAGCTTTGA GTAATCAGAA GTACAGGGAG TTGCATCAGG AGTTTCAAGA TGTTGGATTG 
ATGACAGGAG ATGTTACTCT TTCTCCTAAT GCTAGCTGTT TGGTCATGAC TACGGAGATT 
CTTAGAGGAA TGCTTTATAG GGGTTCTGAG ATTCTGAAGG AAGTTGCTTG GGTTATTTTT 
GATGAGATTC ATTATATGAA GGATCGTGAA AGAGGGGTTG TTTGGGAAGA GAGTATTATA 
TTTATGCCGC CAGAGATTAA GATGGTTTTT CTTTCCGCGA CAATGTCGAA TGCTACTGAG 
TTTGCTGAGT GGATTTGTCA TTTGCATAAG CAGCCATGCC ATGTGGTTTA CACAGATTTT 
CGACCAACTC CTTTGCAGCA TTATGTCTTT CCTGTGGGTG GTGCTGGGTT ATATCTTGTG 
GTTGATGAGA GCGAGCAGTT TAGGGAGGAC AATTTTATGA AGCTGCAGGA TACTTTCTCG 
AAGCAGAAAA TTGGTGAGGG GAATAAGAGT GCAAATGGTA AGGCTAGTGG AAGAATTGCA 
AAGGGTGGGA ATGCTTCTGG GGGTTCTGAC ATATACAAAA TTGTCAAGAT GATCATGGAA 
CGGAAATTTC AACCAGTTAT AGTTTTTAGC TTCAGTAGGA GAGAGGTTGA ACAACATGCC 
ATGTCTATGT CCAAGCTCGA TTTTAATACA CAAGAAGAAA AGGAAATTGT GGAGCAGGTA 
TTTAACAACG CAATACTCTG CTTGAATGAG GAAGACAGGA ATTTGCCTGC TATTGAGTTG 
ATGTTGCCAC TACTTCAGAG AGGCATTGCT GTCCATCATT CTGGTCTTCT CCCTGTTATT 
AAGGAACTAG TAGAGCTTCT TTTCCAGGAA GGCCTTGTTA AGGCTTTATT TGCCACAGAG 
ACATTTGCAA TGGGTTTAAA CATGCCTGCA AAGACTGTTG TTTTCACAGC TGTTAAGAAG 
TGGGATGGTG ACAGTCATCG TTACATTGGA TCTGGTGAGT ATATCCAGAT GAGTGGAAGG 
GCTGGACATC GTGGCAAAGA TGAGCGGGGT ATTTGTATCA TAATGATTGA TGAGCGGATG 
GAGATGAATA CACTCAAAGA CATGGTTTTG GGTAAACCAG CCCCTCTTGT TAGTACTTTC 
AGACTGAGCT ACTATTCAAT CTTGAATTTA ATGAGCCGTG CTGAAGGCCA GTTCACTGCT 
GAACACGTAA TAAGAAATTC ATTTCATCAA TTCCAATATG AGAAGGCTTT ACCAGACATA 
GGGAAAAAGG TTTCAAAACT GGAAGAGGAA GCTGCCATGC TTGATGCTTC GGGAGAGGCT 
GAGGTTGCTG AGTATCATAA TCTAAAACTT GAGATGACCC AANCTTCAAA GAAGATGATG 
ACAGAAATAA CGAGGCCTGA AAGAATTCTT TATTATCTAT GCACTGGTCG GTTGATCAAG 
GTAAGAGAAG GAGGANATTG GGGTTGGGGA GTTGTAGTGA ATGTTGTTAA AAAGCCCACT 
GCAGGTTTGG GAGCATTGCC ATCAAAAGGC GGTGGTTATA TAGTGGATAT TTTACTTCAT 
TGCTCTCCTG GACCAAGTGA GAGTGGCTCT CGGCCAAGAC CATGCCCGCC TCGGCCTGGA 
GAAAAGGGTT GGATGCATGT GGTTCCTGTT CAGTTGCCCC TGATTTGTGC TCTAAGTAAG 
GTAAGGATAT CCATCCCTGC CGACCTTCGG CCTTTGGAAG CAAGACAAAG TATACTACTT 
GCAGTGCAGG AGTTAGGGAA TCGGTTTCCT GAAGGCCTCC CTAAGCTTAA CCCTGTGAAG 
GACATGAAAA TCGAGGATCC TGAAATTGTC GAGTTGGTTA ATCAAATTGA GGAATTGGAA 
CGAAAGCTAC ATGCACATCC ACTGCACAAG TCTCAAGATG TGAATCAGAT GAAAAGTTTT 
CATAGAAAAG CTGAGGTGAA TCATGAAATT CAACAACTCA AATCGAAAAT GCGTGATTCC 
CAGCTTCAAA AATTTCGTGA AGAACTCAAG AACCGATCGT GAGTCTTGAA AAGACTTGGA 
TATATAGATG CTGATGGTGT TGTCCAGCTA AAGGGAAGAG CAGCCTGCTT AATAGACACA 
GGAGATGAAT TACTTGTTAC AGAACTGATG TTCATGGTGA GTAGTTTCAA CGATCTTGAT 
CATCACCAAG TGGCTGCCCT TGCTAGTTGC TTCAATCCAG TAGACAAGTC AAGTGAGCAG 
ATACAGTTGA GAACAGAACT TGCTAAACCA TTACAGCAAC TTCAAGAGAG TGCACGAAAA 
ATAGCAGAGA TACAATATGA ATGCAAATTG GACATAAATG TCGATGAATA TGTAGAATCA 
ACAGTCAGAC CATTCTTGAT GGATGTGATT TACTGTTGGT CAAAGGGAGC TAGTTTTTCA 
GAGGTGATGC AAATGACTGA TATCTTTGAG GGTAGCGTTA TCAGAAGTTC GAGAAGACTA 
GATGAATTTT TGAACCAGTT GCGTGCTGCT GCCCGAGCTG TGGGTGAAGT CAGTTTGGAG 
AGTAAATTTG CAGCTGCCAG CGAAAGCCTT CGTCGAGGTA TCATGTTTGC AAATTCTCTT 
TACCTGCAAG TTGTCTTTTT GCAGGCATTT GTTGTGTGGG GTGCCCATGA GCACAAAAGT 
TCTTACCTTG CAACAATTAT ACCGTGGTGG CCACTTAAGA TGTGCTATAT ACTGGCAACT 
ATAGATTCCA TCTCCTTTTC TCCTTCACTT TTGAGTTTCT TTCATGCTGT TGATCTTATA 
TATTTGACAG TTGATTTCAT TGTATACACA CAAAGTTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC18107847 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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