VCL cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCL
Entrez Gene ID	100432920
Full Name	vinculin
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024254069.1	XP_024109837.1	vinculin isoform X1
XM_009245455.1	XP_009243730.1	LOW QUALITY PROTEIN: vinculin
XM_024254070.1	XP_024109838.1	vinculin isoform X2

Pathways from BioSystems
Gene Ontology

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa164893	XM_024254069.1 Latest version!	Pongo abelii vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa164894	XM_024254070.1 Latest version!	Pongo abelii vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa25569	XM_009245455.1 Latest version!	Pongo abelii vinculin (VCL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCL ( XM_024254069.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024109837.1 Pongo abelii vinculin (VCL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa164893
Clone ID Related Accession (Same CDS sequence)	XM_024254069.1
Accession Version	XM_024254069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519660800000
Organism	Pongo abelii(Sumatran orangutan)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036913.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACAGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTACTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ATTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CCGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCACCTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCAGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGCGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGACCTAA AAGCTCGGAT GCAGGAGGCT ATGACTCAGG AAGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCACCTCC TGATGCGCCT 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTCATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCGAAGGACA TCGCCAAGGC CTCAGACGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAACGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAACGC TCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA 
GCAGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024109837.1
CDS	124..3324
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTTSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_024254069.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024254069.1

ORF Insert Sequence:

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACAGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTACTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ATTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CCGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCACCTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCAGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGCGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGACCTAA AAGCTCGGAT GCAGGAGGCT ATGACTCAGG AAGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCACCTCC TGATGCGCCT 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTCATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCGAAGGACA TCGCCAAGGC CTCAGACGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAACGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAACGC TCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA 
GCAGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_024254070.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024109838.1 Pongo abelii vinculin (VCL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa164894
Clone ID Related Accession (Same CDS sequence)	XM_024254070.1
Accession Version	XM_024254070.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519660800000
Organism	Pongo abelii(Sumatran orangutan)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACAGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTACTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ATTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CCGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCACCTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCAGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGCGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGACCTAA AAGCTCGGAT GCAGGAGGCT ATGACTCAGG AAGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCACCTCC TGATGCGCCT 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG CATCCCAGCC 
GCTGAGGTGG GTATAGGTGT TGCAGCTGAG GCAGATGCGG CCGATGCTGC TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT CATGGCTGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCGAAG 
GACATCGCCA AGGCCTCAGA CGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAAC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ACGCTCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG GGAAGCAGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024109838.1
CDS	124..3528
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTTSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGIPAAEVGIGVAAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_024254070.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024254070.1

ORF Insert Sequence:

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACAGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTACTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ATTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CCGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCACCTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCAGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGCGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGACCTAA AAGCTCGGAT GCAGGAGGCT ATGACTCAGG AAGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCACCTCC TGATGCGCCT 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG CATCCCAGCC 
GCTGAGGTGG GTATAGGTGT TGCAGCTGAG GCAGATGCGG CCGATGCTGC TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT CATGGCTGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCGAAG 
GACATCGCCA AGGCCTCAGA CGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAAC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ACGCTCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG GGAAGCAGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_009245455.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009243730.1 Pongo abelii vinculin (VCL), mRNA.

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CloneID	OSa25569
Clone ID Related Accession (Same CDS sequence)	XM_009245455.1
Accession Version	XM_009245455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1411315200000
Organism	Pongo abelii(Sumatran orangutan)
Product	LOW QUALITY PROTEIN: vinculin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002884430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 5 indels internal stop codons :: corrected 1 genomic stop codons ##RefSeq-Attributes-END##

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACAGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTACTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ATTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAAACTTGTG CAGGCAAAGA ACGGCAGGGA 
GATTCTGGGG AACTTGCAAA ATGCTAGGGG CAGATGACTG ATCAAGTGGC TGACCTCCGT 
GCCAGAGGAC AAGGATCCTC ACCGGTGGCC ATGCAGAAAG CTCAGCAGGT ATCTCAGGGT 
CTGGATGTGC TCACCGCAAA AGTGGAAAAT GCAGCTCGCA AGCTGGAAGC CATGACCACC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAAA ACTGGCTTGC AGATCCAAAT 
GGTGGACCGG AAGGAGAAGA GCAGATTCGA GGTGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAAAGAGAT GACATTCTAC GTTCCCTTGG GGAAATATCT 
GCTCTGACTT CTAAATTAGC AGGCATCTAC GAAGACAGTA TGGGGAAAGG AGATTCTCCA 
GAGGCTCGAG CCTTGGCCAA ACAGGTGGCC ACGGCCCTGC AGAACCTGCA GACCAAAACC 
AACCGGGCTG TGGCCAACAG CAGACCTGCC AAAGCAGCTG TACACCTTGA GGGCAAGATT 
GAGCAAGCAC AGCGGTGGAT TGATAATCCC ACAGTGGATG ACCGTGGAGT CGGTCAGGCT 
GCCATCAGGG GGCTTGTGGC CGAAGGCATC GTCTGGGCTA ATGTTATGAT AGGGCCTTAT 
CGGCAAGATC TTCTCGCCAA GTGTGACCGA GTGGACCAGC TGACAGCCCA GCTGGCTGAC 
CTGGCTGCCA GAGGGGAAGG GGAGAGTCCT CAGGCGCGAG CACTTGCATC TCAGCTCCAA 
GACTCCTTAA AGGTAGAACT CCGGATGCAG GAGGCCATGA CTCAGGAAGT GTCAGATGTT 
TTCAGTGATA CCACAACTCC CATCAAGCTG TTGGCAGTGG CAGCCACGGC ACCTCCTGAT 
GCGCCTAATA GGGAAGAGGT ATTTGATGAG AGGGCAGCTA ACTTTGAAAA CCATTCAGGA 
AAGCTTGGTG CTACGGCCGA GAAGGCGGCT GCGGTTGGTA CTGCTAATAA ATCAACAGTG 
GAAGGCATTC AGGCCTCAGT GAAGACGGCC CGAGAACTCA CACCCCAGGT GGTCTCGGCT 
GCTCGTATCT TACTTAGGAA CCCTGGAAAT CAAGCTGCTT ATGAACATTT TGAGACCATG 
AAGAACCAGT GGATCGATAA TGTTGAAAAA ATGACAGGGC TGGTGGACGA AGCCATTGAT 
ACCAAATCTC TGTTGGATGC TTCAGAAGAA GCAATTAAAA AAGACCTGGA CAAGTGCAAG 
GTAGCTATGG CCAACATTCA GCCTCAGATG CTGGTTGCTG GGGCAACCAG CATTGCTCGT 
CGGGCCAACC GGATCCTGCT GGTGGCTAAG AGGGAGGTGG AGAATTCCGA GGATCCCAAG 
TTCCGTGAGG CTGTGAAAGC TGCCTCTGAT GAATTGAGCA AAACCATCTC CCCGATGGTG 
ATGGATGCAA AAGCTGTGGC TGGAAACATT TCTGACCCTG GACTGCAAAA GAGCTTCCTG 
GACTCAGGAT ATCGGATCCT GGGAGCTGTG GCCAAGGTCA GAGAAGCCTT CCAACCTCAG 
GAGCCTGACT TCCCGCCGCC TCCACCAGAC CTTGAACAAC TCCGACTAAC AGATGAGCTT 
GCTCCTCCCA AACCACCTCT GCCTGAAGGT GAGGTCCCTC CACCTAGGCC TCCACCACCA 
GAGGAAAAGG ATGAAGAGTT CCCTGAGCAG AAGGCCGGGG AGGTGATTAA CCAGCCAATG 
ATGATGGCTG CCAGACAGCT CCATGATGAA GCTCGCAAAT GGTCCAGCAA GCCGGGCATC 
CCAGCCGCTG AGGTGGGTAT AGGTGTTGCA GCTGAGGCAG ATGCGGCCGA TGCTGCTGGG 
TTCCCTGTCC CCCCTGACAT GGAAGACGAT TACGAACCTG AGCTGCTGTT AATGCCATCC 
AATCAGCCGG TCAACCAGCC CATTCTGGCC GCGGCTCAGT CCTTGCATCG GGAAGCTACC 
AAGTGGTCTA GTAAGGGCAA TGACATCATT GCAGCAGCCA AGCGCATGGC TCTGCTCATG 
GCTGAGATGT CTCGGCTGGT AAGAGGGGGC AGTGGTACCA AGCGGGCACT CATTCAGTGT 
GCGAAGGACA TCGCCAAGGC CTCAGACGAG GTGACTCGGT TGGCCAAGGA GGTTGCCAAG 
CAGTGCACAG ATAAACGGAT TAGAACCAAC CTCTTACAGG TATGTGAGCG AATCCCAACC 
ATAAGCACCC AGCTCAAAAT CCTGTCCACA GTGAAGGCCA CCATGCTGGG CCGGACCAAC 
ATCAGTGATG AGGAGTCTGA GCAGGCCACA GAGATGCTGG TTCACAACGC TCAGAACCTC 
ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA GCAGAAGCTG CTTCAATCAA AATTCGAACA 
GATGCTGGAT TTACACTGCG CTGGGTTAGA AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009243730.1
CDS	244..3654
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGETCAGKERQGDSGELAKC*GQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTTSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLAGIYEDSMGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGIVWANVMIGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKVELRMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGIPAAEVGIGVAAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_009245455.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii vinculin (VCL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009245455.1

ORF Insert Sequence:

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACAGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTACTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ATTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAAACTTGTG CAGGCAAAGA ACGGCAGGGA 
GATTCTGGGG AACTTGCAAA ATGCTAGGGG CAGATGACTG ATCAAGTGGC TGACCTCCGT 
GCCAGAGGAC AAGGATCCTC ACCGGTGGCC ATGCAGAAAG CTCAGCAGGT ATCTCAGGGT 
CTGGATGTGC TCACCGCAAA AGTGGAAAAT GCAGCTCGCA AGCTGGAAGC CATGACCACC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAAA ACTGGCTTGC AGATCCAAAT 
GGTGGACCGG AAGGAGAAGA GCAGATTCGA GGTGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAAAGAGAT GACATTCTAC GTTCCCTTGG GGAAATATCT 
GCTCTGACTT CTAAATTAGC AGGCATCTAC GAAGACAGTA TGGGGAAAGG AGATTCTCCA 
GAGGCTCGAG CCTTGGCCAA ACAGGTGGCC ACGGCCCTGC AGAACCTGCA GACCAAAACC 
AACCGGGCTG TGGCCAACAG CAGACCTGCC AAAGCAGCTG TACACCTTGA GGGCAAGATT 
GAGCAAGCAC AGCGGTGGAT TGATAATCCC ACAGTGGATG ACCGTGGAGT CGGTCAGGCT 
GCCATCAGGG GGCTTGTGGC CGAAGGCATC GTCTGGGCTA ATGTTATGAT AGGGCCTTAT 
CGGCAAGATC TTCTCGCCAA GTGTGACCGA GTGGACCAGC TGACAGCCCA GCTGGCTGAC 
CTGGCTGCCA GAGGGGAAGG GGAGAGTCCT CAGGCGCGAG CACTTGCATC TCAGCTCCAA 
GACTCCTTAA AGGTAGAACT CCGGATGCAG GAGGCCATGA CTCAGGAAGT GTCAGATGTT 
TTCAGTGATA CCACAACTCC CATCAAGCTG TTGGCAGTGG CAGCCACGGC ACCTCCTGAT 
GCGCCTAATA GGGAAGAGGT ATTTGATGAG AGGGCAGCTA ACTTTGAAAA CCATTCAGGA 
AAGCTTGGTG CTACGGCCGA GAAGGCGGCT GCGGTTGGTA CTGCTAATAA ATCAACAGTG 
GAAGGCATTC AGGCCTCAGT GAAGACGGCC CGAGAACTCA CACCCCAGGT GGTCTCGGCT 
GCTCGTATCT TACTTAGGAA CCCTGGAAAT CAAGCTGCTT ATGAACATTT TGAGACCATG 
AAGAACCAGT GGATCGATAA TGTTGAAAAA ATGACAGGGC TGGTGGACGA AGCCATTGAT 
ACCAAATCTC TGTTGGATGC TTCAGAAGAA GCAATTAAAA AAGACCTGGA CAAGTGCAAG 
GTAGCTATGG CCAACATTCA GCCTCAGATG CTGGTTGCTG GGGCAACCAG CATTGCTCGT 
CGGGCCAACC GGATCCTGCT GGTGGCTAAG AGGGAGGTGG AGAATTCCGA GGATCCCAAG 
TTCCGTGAGG CTGTGAAAGC TGCCTCTGAT GAATTGAGCA AAACCATCTC CCCGATGGTG 
ATGGATGCAA AAGCTGTGGC TGGAAACATT TCTGACCCTG GACTGCAAAA GAGCTTCCTG 
GACTCAGGAT ATCGGATCCT GGGAGCTGTG GCCAAGGTCA GAGAAGCCTT CCAACCTCAG 
GAGCCTGACT TCCCGCCGCC TCCACCAGAC CTTGAACAAC TCCGACTAAC AGATGAGCTT 
GCTCCTCCCA AACCACCTCT GCCTGAAGGT GAGGTCCCTC CACCTAGGCC TCCACCACCA 
GAGGAAAAGG ATGAAGAGTT CCCTGAGCAG AAGGCCGGGG AGGTGATTAA CCAGCCAATG 
ATGATGGCTG CCAGACAGCT CCATGATGAA GCTCGCAAAT GGTCCAGCAA GCCGGGCATC 
CCAGCCGCTG AGGTGGGTAT AGGTGTTGCA GCTGAGGCAG ATGCGGCCGA TGCTGCTGGG 
TTCCCTGTCC CCCCTGACAT GGAAGACGAT TACGAACCTG AGCTGCTGTT AATGCCATCC 
AATCAGCCGG TCAACCAGCC CATTCTGGCC GCGGCTCAGT CCTTGCATCG GGAAGCTACC 
AAGTGGTCTA GTAAGGGCAA TGACATCATT GCAGCAGCCA AGCGCATGGC TCTGCTCATG 
GCTGAGATGT CTCGGCTGGT AAGAGGGGGC AGTGGTACCA AGCGGGCACT CATTCAGTGT 
GCGAAGGACA TCGCCAAGGC CTCAGACGAG GTGACTCGGT TGGCCAAGGA GGTTGCCAAG 
CAGTGCACAG ATAAACGGAT TAGAACCAAC CTCTTACAGG TATGTGAGCG AATCCCAACC 
ATAAGCACCC AGCTCAAAAT CCTGTCCACA GTGAAGGCCA CCATGCTGGG CCGGACCAAC 
ATCAGTGATG AGGAGTCTGA GCAGGCCACA GAGATGCTGG TTCACAACGC TCAGAACCTC 
ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA GCAGAAGCTG CTTCAATCAA AATTCGAACA 
GATGCTGGAT TTACACTGCG CTGGGTTAGA AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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VCL cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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