PF3D7_0808600 cDNA ORF clone, Plasmodium falciparum 3D7

Gene
Clones
gRNAs

Summary

Gene Symbol	PF3D7_0808600
Entrez Gene ID	4794195
Full Name	erythrocyte membrane protein 1, PfEMP1
Gene Type	protein-coding
Organism	Plasmodium falciparum 3D7

mRNA and Protein(s)

mRNA	Protein	Name
XM_001349402.1	XP_001349438.1	erythrocyte membrane protein 1, PfEMP1

Pathways from BioSystems
Gene Ontology

The following PF3D7_0808600 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PF3D7_0808600 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr05055	XM_001349402.1 Latest version!	Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_0808600), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PF3D7_0808600 ( XM_001349402.1 ) cDNA ORF clone, Plasmodium falciparum 3D7 -> XP_001349438.1 Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_0808600), partial mRNA.

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CloneID	OPr05055
Clone ID Related Accession (Same CDS sequence)	XM_001349402.1
Accession Version	XM_001349402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1522080000000
Organism	Plasmodium falciparum 3D7
Product	erythrocyte membrane protein 1, PfEMP1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_004329). COMPLETENESS: incomplete on both ends.

ATGCCGCCTC TAGGTCGTCA GGTTGGTGGT AGTCCACAGG ATGATGATGC CAAAAATATG 
TTTGATAGGA TAGGGAAAGA CGTGTACGAC CAAGTGAAAA AGGGTGTTGC TGAAACATAT 
AAAGAGGCGT TGACAGGACA GTTGTCACTA GCAACACTTT TGGGTGTGGA ATCAGCTAGC 
ACCACAGATC CATGCGGACT TGATTATAGT AAACTTATTA GTGGTAGTGG TGTTGCTGCT 
CGCGGTCATC CGTGCGGAAA TGAAAGTGTA AGTGAAAAAC GTTTTTCTAA AGAACGTGTC 
GATGAATATG ATGAGAAAAA AATAAAAGAT AATAAAGGTA ATAGAGGTAA TAATGAAGGA 
GAGTGCGCTC CATATAGAAG GTTATCTCTA TGTAACAAAA ATTTTCAAAA AATCAATAAT 
ATTGATAGTG ATAAAGCTAG ACATAATTTA TTGGTAGATG TGTGTCTTGC AGCACACTAT 
GAAGGGGACT CAATAAAAGC ACATCTTGAA CAATATGATG CAACATATCC TGGTTCTGGT 
TCTACAACAT GTACTGCACT TGCACGAAGT TTTGCAGATA TAGGTGATAT CGTTAGAGGA 
AGAGATCTGT ATCGTGGTAA TAAAAAAAAA ATTAAAACAG AAACAGAAAG AGATAAATTA 
GAACAGAAAT TGAAAGAAAT TTTCAAGAAC ATTAAGAAAG AGAATAATGA AAAACTTAAA 
TCCCTTACAG ATGACCAAAT TAGGGAATAT TGGTGGACTG CGAATCGGCA CACCGTGTGG 
AAAGCCATCA CATGTAAGGC GGACGCAAGT AGTGCATATT TTCATGCAAC GTGCAGTGAT 
AGTCATCGTA GTGGAACTTT TTCTCAAGCT AATAACTACT GCCGGTGTAA CGGCGACCAG 
CCAGGTAACG ACAAGGAAAA TATCGATCCC CCAACCTATT TCGATTATGT GCCTCAATAT 
TTGAGGTGGT TCGAGGAATG GGCAGAAGAC TTTTGTCGTA AAAAAAAACA CAAATTGAAC 
GATGCGATAC AAAAATGTCG TGGAGAAGAT AAATATGGTA AGGATCGATA TTGTGATCTT 
AATGGATATG ATTGCGAAAA AACCAAAAGA GGAAGAAACA TGTATCGTTG GGATCACAAA 
TGTACTGGCT GTTTCCTTTC TTGCTCTCAT TTTAGAACAT GGATAGATAA CCAAAAACTA 
GAATTTCTAA AACAAAAAGA AAAATATACA AATGAAATAT CAGGTGGTAA AAGCAGGAAA 
AAACGAGCTG CACGTAGTAG TAGTAGTAGT AGTTATGATA ATGGATATGA AAAAAAATTT 
TATGACCAAC TGAAAGCAGG TGGCTATAAT GGTGTTAATA GTTTTTTGGA TTTATTAAGT 
AAAGAAAAAA CATGCAAAGA TATTACTGAC ACAGAAGGAG GAAAAATTGA TTTCAAAAAC 
GTAAATAGTG ATAAAAATAG CTATGACGAT GATAGTAATA AAACATTTTC TCATACAGAA 
TATTGTCAAG CATGTCCTCT ATGTGGGGTC AAACGTAATG GTAGGAAATG GGAAAGGAAA 
GAGGTTATGA AGGATTGTCC GCCTATAAAC CTTTATAAGC CTAAAAAGGA CGCAGAAGGT 
ACTCTTATTA ATTTCCTGTA CAGTGGTGAT GAAACTAATG AAATTGCAAA AAAGCTAAAA 
GCGTTTTGCG CTCAAGCAAA TGGTGATACA ACAAATGGTA CTGGTGGTAA TGGTACTGGT 
GGTAGTGTTG CTGGAGGTAC TGGCACTAGT GGTAGTAACG AATTGTATCA AAAATGGAAA 
TGTTATGAAA TTGACGAGCT GACGAAAGAT CAAAAAGAAG GGGGTGAGGA TGACCCAGTG 
TATGACGAGG ATGTAAAAAC TGGAGGCGGA TTATGTATAT TGGAAAACAA AAATAAAAGC 
AAAGGAAGTC AAAGCAATTC TCAAAAGGAA CCTGATGAAA TCCAAAAGAC ATTCAATCCT 
TTTTTTTACT ATTGGGTGGT ACATATGTTA AAAGATTCCA TACACTGGAG AACAAAAAAA 
CTTGATAAGT GTATAAATAA CAGTAATGAA TCAAAAGCAT GTAAAAACAA TAATAAATGT 
AAGGATGATT GTGGTTGTTT TTTAAAATGG GTTGTACAAA AAAAAACCGA ATGGGAAAAT 
ATAAAAAAAC ATTTTAAAAA GCAAAAAGAT ATTCCACCGG GATTTACTCA CGATGATGTT 
CTTGAAGGTG TTTTGGAGAA AGAGGTACTT TTGACAAGTA TTAAAGAGGG TTATGGGAAT 
GAAAAGGACA TAAAACACAT TAAGAAACTG TTGGATGAGG AAGAAGCAGC TGGTGTAACC 
GACAATGAAA ATAAGACCAC AATTGATAAA TTACTAAAAC ACGAAAAAGA CGAAGCAGAT 
AAATGCAAAC AAATTCAAGA AGAATGCAAT AAACAAAAAC AACAAGAACG AGGTGGCCCT 
GGAGGTCGTT CCGCCGACCC CTCACCACCT GCTGACCTCG ACGAACACTC CTCAGACGAA 
GGTGAAGAAG ACGACGACGA CGACGAAGAA GAGGAAAACG CCAACGAGGA AACCGCAGAG 
GACGCCACAG AAGACACACA AGTGGATGGG ACAGAGGTCG TAGAAGAAAC CGTCGCAGAG 
GTAACAGAGG TAACAGAGGT GAAGCCATGT GAAATAGTAC AAAAACTATT TACTAATGGC 
GACCTACAAA ACACTTTTAA AGATGCGTGT GAACAAAAAT ATGGTTACCC ACAACGACAT 
TGGGGGTGGA AATGTATAAG TGATACAACC ACTGGCAAAA GTGGTGATAA CACCGGTAGT 
AGTGGTGCTA TATGTATTCC CCCGAGACGT CGCAAACTAT ATATAGGTAA AATAAAGGAG 
TGGGCAAACA GTGGTAACAC ACAGGCGGCA GAACCACAGG AGGATGGTAA GGCACAGACA 
CCACAAGGTC AAACACCATC ACAAAGTGGT AAAGAGTCGT CACAAAGTCA GAAATTGCGT 
GAGGCGTTCA TCCAATCTGC TGCAATAGAA ACTTTTTTCC TATGGGATAG ATATAAAAAG 
GTTAAAAATA AGGAAATAGC TGAAAAAAAA CAACGAGATA GTACAAACCC ATTTGGCTTC 
ACCCAGTCAA CATCAGGTGG AATGCAAGCA TTACCAGTTG GAGGAGCAGT ACAGGGAGCA 
CAAGCACAAC TACCCGATGG TGCCGGTGGT AGTGACCAAA CTCCCGAAAA ACAATTGGCT 
GGTGGCAAAA TACCTGAGGT TTTCTTACGT CAGATGTTTT ATACGTTAGG GGATTATAGA 
GATATATGTG TGGGGAACAC ACCTAGTGGT ATAGATACAA ATGATAAAGA AAATATGCAA 
AAAATACAAA ATAAAATAAA AAGTGTTATT GAAAAAAGTG ATTCCACACC TCGCACACCT 
GGTACCCACT CCCCATCTAG TGGTACAACC CCTCAAGCCT TGTGGGATAA AATCTCCCCT 
TCTATTTGGG AAGGAATGTT ATGTGCTTTA ACATACAAAG ATGGCGGAGA GGGCAAAAAA 
ATAGAAAAGG TTAACGATGC GAACGGCGAC GACCTTTTCC AAAAACTGAA AGACAAATAC 
AGCGACTACA AGACAGTCAC ACTCGAAGAA GATAATACCT CCTCCGCCAT GTCTACCAGT 
CCCAGAACCT CCGAAACCAC CTCAGCCTCT AGTGATAACA CCCCCACCCT CAACAACCCC 
AAATTGAGTG ATTTCGTGTT ACGCCCCCCC TACTTCCGAT ACCTTGAAGA ATGGGGTCAA 
AATTTTTGTA AAAAACGAAC AGAGATGTTG GAGAAGATAA AGGAGGAATG TCGCAGTGGC 
ACAGGAGGTC ATGAATATTG TAGTGGGGAT GGACATGATT GTACGGATAA TGATCGTAAA 
TATAATAAAA TGATTGCAGA TTTTCATTGT CCAGGTTGTG CAAAAGAATG TATGAAATAT 
AAAACATGGA TAGGAAAAAA ATTCGAAGAA TTTAAGAAGC AAGAAAATAC ATATAAAGTG 
GAACATGGTA AATTAACGAA TAATAATTGT AGTGGTAGTG ATAATACAAA ATTTTGTCAA 
CAAATAAAAA ATAATTCTTT TGATAAATTT TTGGAATTAT TGAGACATTG CAAAGATGGT 
AAGGATGATA CGGATAAAGA TAATGAATTA AATTTTAGTA AGCCTCTCCA AACCTTTTCT 
CGTTCAACGT ATTGTAAAGC GTGTCCTATA TATGGAGTTA AGTATAATAG GGGAACATAT 
AGCGCTATTA ATGAAAGTGA ATATATGAGC AAAAATGGTA TTAGTGGAGA AAATAAGAAC 
GATAAGAAAC CTACGGAAAT TAAAGTACTT CTGTTAGGTC GGAAAGGAGA AGTTAGGAAT 
AATGATGAAG AACTTAAAGA ACTTAAAGAA GTTTGTAATA ATGCCGGTTT TGTTGAGGAT 
TATAGTCTTC AAAAATGGAA CTGCCAAAAA AAGAATGGAG TGGATCAGTG TAATCTTACA 
AATTCTGTGG ATAATATAGA TGATAGTGAT AAAATTATTC CGTTTAATGT TTTTTTTCAG 
CGTTGGTTAA GAAATTTTGT ACACGATTAT AATAAATTGA AACAGAAAAT TCATCCATGT 
ATAAAAAATG AAGATGGAAA GGAACATAAA TGTATTGAAG GATGTAAAAA AAAATGTGAA 
TGTGTAGAAA AATGGTTAGA TAAAAAATCT ACAGAATGGG ATGAAATAAA AAAACATTAT 
GAGAAACATT CAAAAGATCA TAATGAAGGT ATTCCTCATA GTGTTAAAGG TTATCTTGTA 
CAGGCACCGT TTAAGGATGA AGTTCTCAAA GCTATAAAAC CTTGTGATTT TGATAACTTC 
AAGACTTCGT GTGGTCTTAA TGGCACTGAT AATTCACAAA ATGGTAATAA TAACGATCTT 
GTTTTATGTA TGATTAAAAA CCTTGAAAAA AAAATTGAAC AATGCAAAAA GAAACACGAC 
GAAAACAAAA AAACACCGTG TGATCCATTA CCCCCTCTAC CTGACGAAAA CGATGAAACC 
CTTGAAGAAA ACCCAGTGGC GCAACCGAAC ATTTGTCCGC AACTACCAGA GGAACCAAAA 
GAGACTTGTG AAGAAGCAGT CACTCCTAGT GAACCAAAAA AAGCGGAAGA AGAACCAGCA 
GCAGAACCCG AAGCCGTGCC CGAAGAAGAA GCACCGTTAC CTCCACCTCC CCCTGCGGCC 
CCACCTCGTG AGAGCAAAGA AAAAGCAAAA CCCCCCCCAA AACCTCGAAT CAAAACCCGT 
CATGTATTGG ACCACCCCGC TGTCATACCC GCCCTCATGT CTTCTACCAT CATGTGGAGT 
ATTGGCATCG GTTTTGCTGC GTTCACTTAT TTTTTTCTAA AGAAAAAAAC CAAATCATCT 
GTTGGAAATT TATTTCAAAT ACTGCAAATA CCCAAAAGTA ATTATGATAT ACCGACAAAA 
CGTTCACCCA ATAGATATAT ACCTTATACT AGTGGTAAAT ACAGAGGCAA ACGGTACATT 
TACCTTGAAG GAGATAGTGG AACAGATAGT GGTTACACCG ATCATTATAG TGATATTACT 
TCATCTTCCG AAAGTGAATA TGAAGAGATG GATATCAATG ATATATATGC ACCACGTGCT 
CCTAAATATA AAACGTTGAT AGAAGTGGTA CTTGAACCTA GTGGTAACAA CACAACAGCT 
AGTGGTAAAA ACACACCTAG TGATACACAA AATGATATAC CAACTAGTGA TACACCACCA 
CCCATTACTG ATGATGAGTG GAATACATTG AAACATGATT TTATATCTCA ATATTTACCA 
AATACACAAA ATACGGAACC AAATATTTTA CATGATAATT TGGATAATAA TACCCATCCC 
ACCCCGTCAC GTCATACGTT GGACCAAAAA CCTTTTATTA TGTCTATTCA TGATAGGGAT 
TTATATAGTG GAGAAGAAAT TAGTTATAAT ATTCATATGA GTACTAATAG TATGGATGAT 
CCAAAATATG TATCAAATAA TGTATATTCT GGTATCGATT TAATTAATGA CACATTAAGT 
GGTAACAAAC ATATTGATAT ATATGATGAA GTTTTGAAAA GAAAAGAAAA CGAATTATTT 
GGAACAAATT ATAAGAAAAA TACATCAAAC AATAGTGTTA CGAAAAATAT TAATAGTGAT 
CCTATAGATA ATCAGTTGAA TTTGTTTCAT ACATGGTTAG ATAGACATAG AGATATGTGT 
GAACAATGGA ATAATAAAGA GGAACTATTA GATAAATTGA AAGAAGAGTG GGAAAATGAG 
ACACATAGTG GTAACAAACA TAGTGACATA CCTAGTGGTA AACTAAGTGA TACACCTAGT 
GATAACAACA TACATAGTGA TATTCATCCT AGTGATATAC CTAGTGGTAA ACAAAGTGAT 
ATACCTAGTG ATAACAACAT ACATAGTGAC ATACCATACG TGTTAAATAC TGATGTTTCC 
ATACAAATAC ATATGGATAA TCCCAAAACT ACAAATGAGT TTACATATGT GGATAGTAAC 
CCCAACCAAG TGGATGACAC CTACGTGGAT AGTAACCCTG ACAATTCTTC CATGGATACT 
ATCTTGGACG ATCTGGAAAA ATATAATGAA CCTTATTATG ATGTACAAGA TGATATTTAT 
TATGATGTAA ATGATCATGA TACATCAACT GTGGATAGTA ATGCTATGGA TATACCTAGC 
AAAGTACAAA TTGAAATGGA TGTAAATACC AAATTGGTGA AAGAGAAATA TCCTATAGGA 
GATGTGTGGG ATATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001349438.1
CDS	1..6798
Translation	MPPLGRQVGGSPQDDDAKNMFDRIGKDVYDQVKKGVAETYKEALTGQLSLATLLGVESASTTDPCGLDYSKLISGSGVAARGHPCGNESVSEKRFSKERVDEYDEKKIKDNKGNRGNNEGECAPYRRLSLCNKNFQKINNIDSDKARHNLLVDVCLAAHYEGDSIKAHLEQYDATYPGSGSTTCTALARSFADIGDIVRGRDLYRGNKKKIKTETERDKLEQKLKEIFKNIKKENNEKLKSLTDDQIREYWWTANRHTVWKAITCKADASSAYFHATCSDSHRSGTFSQANNYCRCNGDQPGNDKENIDPPTYFDYVPQYLRWFEEWAEDFCRKKKHKLNDAIQKCRGEDKYGKDRYCDLNGYDCEKTKRGRNMYRWDHKCTGCFLSCSHFRTWIDNQKLEFLKQKEKYTNEISGGKSRKKRAARSSSSSSYDNGYEKKFYDQLKAGGYNGVNSFLDLLSKEKTCKDITDTEGGKIDFKNVNSDKNSYDDDSNKTFSHTEYCQACPLCGVKRNGRKWERKEVMKDCPPINLYKPKKDAEGTLINFLYSGDETNEIAKKLKAFCAQANGDTTNGTGGNGTGGSVAGGTGTSGSNELYQKWKCYEIDELTKDQKEGGEDDPVYDEDVKTGGGLCILENKNKSKGSQSNSQKEPDEIQKTFNPFFYYWVVHMLKDSIHWRTKKLDKCINNSNESKACKNNNKCKDDCGCFLKWVVQKKTEWENIKKHFKKQKDIPPGFTHDDVLEGVLEKEVLLTSIKEGYGNEKDIKHIKKLLDEEEAAGVTDNENKTTIDKLLKHEKDEADKCKQIQEECNKQKQQERGGPGGRSADPSPPADLDEHSSDEGEEDDDDDEEEENANEETAEDATEDTQVDGTEVVEETVAEVTEVTEVKPCEIVQKLFTNGDLQNTFKDACEQKYGYPQRHWGWKCISDTTTGKSGDNTGSSGAICIPPRRRKLYIGKIKEWANSGNTQAAEPQEDGKAQTPQGQTPSQSGKESSQSQKLREAFIQSAAIETFFLWDRYKKVKNKEIAEKKQRDSTNPFGFTQSTSGGMQALPVGGAVQGAQAQLPDGAGGSDQTPEKQLAGGKIPEVFLRQMFYTLGDYRDICVGNTPSGIDTNDKENMQKIQNKIKSVIEKSDSTPRTPGTHSPSSGTTPQALWDKISPSIWEGMLCALTYKDGGEGKKIEKVNDANGDDLFQKLKDKYSDYKTVTLEEDNTSSAMSTSPRTSETTSASSDNTPTLNNPKLSDFVLRPPYFRYLEEWGQNFCKKRTEMLEKIKEECRSGTGGHEYCSGDGHDCTDNDRKYNKMIADFHCPGCAKECMKYKTWIGKKFEEFKKQENTYKVEHGKLTNNNCSGSDNTKFCQQIKNNSFDKFLELLRHCKDGKDDTDKDNELNFSKPLQTFSRSTYCKACPIYGVKYNRGTYSAINESEYMSKNGISGENKNDKKPTEIKVLLLGRKGEVRNNDEELKELKEVCNNAGFVEDYSLQKWNCQKKNGVDQCNLTNSVDNIDDSDKIIPFNVFFQRWLRNFVHDYNKLKQKIHPCIKNEDGKEHKCIEGCKKKCECVEKWLDKKSTEWDEIKKHYEKHSKDHNEGIPHSVKGYLVQAPFKDEVLKAIKPCDFDNFKTSCGLNGTDNSQNGNNNDLVLCMIKNLEKKIEQCKKKHDENKKTPCDPLPPLPDENDETLEENPVAQPNICPQLPEEPKETCEEAVTPSEPKKAEEEPAAEPEAVPEEEAPLPPPPPAAPPRESKEKAKPPPKPRIKTRHVLDHPAVIPALMSSTIMWSIGIGFAAFTYFFLKKKTKSSVGNLFQILQIPKSNYDIPTKRSPNRYIPYTSGKYRGKRYIYLEGDSGTDSGYTDHYSDITSSSESEYEEMDINDIYAPRAPKYKTLIEVVLEPSGNNTTASGKNTPSDTQNDIPTSDTPPPITDDEWNTLKHDFISQYLPNTQNTEPNILHDNLDNNTHPTPSRHTLDQKPFIMSIHDRDLYSGEEISYNIHMSTNSMDDPKYVSNNVYSGIDLINDTLSGNKHIDIYDEVLKRKENELFGTNYKKNTSNNSVTKNINSDPIDNQLNLFHTWLDRHRDMCEQWNNKEELLDKLKEEWENETHSGNKHSDIPSGKLSDTPSDNNIHSDIHPSDIPSGKQSDIPSDNNIHSDIPYVLNTDVSIQIHMDNPKTTNEFTYVDSNPNQVDDTYVDSNPDNSSMDTILDDLEKYNEPYYDVQDDIYYDVNDHDTSTVDSNAMDIPSKVQIEMDVNTKLVKEKYPIGDVWDI

Target ORF information:

RefSeq Version	XM_001349402.1
Organism	Plasmodium falciparum 3D7
Definition	Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_0808600), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001349402.1

ORF Insert Sequence:

ATGCCGCCTC TAGGTCGTCA GGTTGGTGGT AGTCCACAGG ATGATGATGC CAAAAATATG 
TTTGATAGGA TAGGGAAAGA CGTGTACGAC CAAGTGAAAA AGGGTGTTGC TGAAACATAT 
AAAGAGGCGT TGACAGGACA GTTGTCACTA GCAACACTTT TGGGTGTGGA ATCAGCTAGC 
ACCACAGATC CATGCGGACT TGATTATAGT AAACTTATTA GTGGTAGTGG TGTTGCTGCT 
CGCGGTCATC CGTGCGGAAA TGAAAGTGTA AGTGAAAAAC GTTTTTCTAA AGAACGTGTC 
GATGAATATG ATGAGAAAAA AATAAAAGAT AATAAAGGTA ATAGAGGTAA TAATGAAGGA 
GAGTGCGCTC CATATAGAAG GTTATCTCTA TGTAACAAAA ATTTTCAAAA AATCAATAAT 
ATTGATAGTG ATAAAGCTAG ACATAATTTA TTGGTAGATG TGTGTCTTGC AGCACACTAT 
GAAGGGGACT CAATAAAAGC ACATCTTGAA CAATATGATG CAACATATCC TGGTTCTGGT 
TCTACAACAT GTACTGCACT TGCACGAAGT TTTGCAGATA TAGGTGATAT CGTTAGAGGA 
AGAGATCTGT ATCGTGGTAA TAAAAAAAAA ATTAAAACAG AAACAGAAAG AGATAAATTA 
GAACAGAAAT TGAAAGAAAT TTTCAAGAAC ATTAAGAAAG AGAATAATGA AAAACTTAAA 
TCCCTTACAG ATGACCAAAT TAGGGAATAT TGGTGGACTG CGAATCGGCA CACCGTGTGG 
AAAGCCATCA CATGTAAGGC GGACGCAAGT AGTGCATATT TTCATGCAAC GTGCAGTGAT 
AGTCATCGTA GTGGAACTTT TTCTCAAGCT AATAACTACT GCCGGTGTAA CGGCGACCAG 
CCAGGTAACG ACAAGGAAAA TATCGATCCC CCAACCTATT TCGATTATGT GCCTCAATAT 
TTGAGGTGGT TCGAGGAATG GGCAGAAGAC TTTTGTCGTA AAAAAAAACA CAAATTGAAC 
GATGCGATAC AAAAATGTCG TGGAGAAGAT AAATATGGTA AGGATCGATA TTGTGATCTT 
AATGGATATG ATTGCGAAAA AACCAAAAGA GGAAGAAACA TGTATCGTTG GGATCACAAA 
TGTACTGGCT GTTTCCTTTC TTGCTCTCAT TTTAGAACAT GGATAGATAA CCAAAAACTA 
GAATTTCTAA AACAAAAAGA AAAATATACA AATGAAATAT CAGGTGGTAA AAGCAGGAAA 
AAACGAGCTG CACGTAGTAG TAGTAGTAGT AGTTATGATA ATGGATATGA AAAAAAATTT 
TATGACCAAC TGAAAGCAGG TGGCTATAAT GGTGTTAATA GTTTTTTGGA TTTATTAAGT 
AAAGAAAAAA CATGCAAAGA TATTACTGAC ACAGAAGGAG GAAAAATTGA TTTCAAAAAC 
GTAAATAGTG ATAAAAATAG CTATGACGAT GATAGTAATA AAACATTTTC TCATACAGAA 
TATTGTCAAG CATGTCCTCT ATGTGGGGTC AAACGTAATG GTAGGAAATG GGAAAGGAAA 
GAGGTTATGA AGGATTGTCC GCCTATAAAC CTTTATAAGC CTAAAAAGGA CGCAGAAGGT 
ACTCTTATTA ATTTCCTGTA CAGTGGTGAT GAAACTAATG AAATTGCAAA AAAGCTAAAA 
GCGTTTTGCG CTCAAGCAAA TGGTGATACA ACAAATGGTA CTGGTGGTAA TGGTACTGGT 
GGTAGTGTTG CTGGAGGTAC TGGCACTAGT GGTAGTAACG AATTGTATCA AAAATGGAAA 
TGTTATGAAA TTGACGAGCT GACGAAAGAT CAAAAAGAAG GGGGTGAGGA TGACCCAGTG 
TATGACGAGG ATGTAAAAAC TGGAGGCGGA TTATGTATAT TGGAAAACAA AAATAAAAGC 
AAAGGAAGTC AAAGCAATTC TCAAAAGGAA CCTGATGAAA TCCAAAAGAC ATTCAATCCT 
TTTTTTTACT ATTGGGTGGT ACATATGTTA AAAGATTCCA TACACTGGAG AACAAAAAAA 
CTTGATAAGT GTATAAATAA CAGTAATGAA TCAAAAGCAT GTAAAAACAA TAATAAATGT 
AAGGATGATT GTGGTTGTTT TTTAAAATGG GTTGTACAAA AAAAAACCGA ATGGGAAAAT 
ATAAAAAAAC ATTTTAAAAA GCAAAAAGAT ATTCCACCGG GATTTACTCA CGATGATGTT 
CTTGAAGGTG TTTTGGAGAA AGAGGTACTT TTGACAAGTA TTAAAGAGGG TTATGGGAAT 
GAAAAGGACA TAAAACACAT TAAGAAACTG TTGGATGAGG AAGAAGCAGC TGGTGTAACC 
GACAATGAAA ATAAGACCAC AATTGATAAA TTACTAAAAC ACGAAAAAGA CGAAGCAGAT 
AAATGCAAAC AAATTCAAGA AGAATGCAAT AAACAAAAAC AACAAGAACG AGGTGGCCCT 
GGAGGTCGTT CCGCCGACCC CTCACCACCT GCTGACCTCG ACGAACACTC CTCAGACGAA 
GGTGAAGAAG ACGACGACGA CGACGAAGAA GAGGAAAACG CCAACGAGGA AACCGCAGAG 
GACGCCACAG AAGACACACA AGTGGATGGG ACAGAGGTCG TAGAAGAAAC CGTCGCAGAG 
GTAACAGAGG TAACAGAGGT GAAGCCATGT GAAATAGTAC AAAAACTATT TACTAATGGC 
GACCTACAAA ACACTTTTAA AGATGCGTGT GAACAAAAAT ATGGTTACCC ACAACGACAT 
TGGGGGTGGA AATGTATAAG TGATACAACC ACTGGCAAAA GTGGTGATAA CACCGGTAGT 
AGTGGTGCTA TATGTATTCC CCCGAGACGT CGCAAACTAT ATATAGGTAA AATAAAGGAG 
TGGGCAAACA GTGGTAACAC ACAGGCGGCA GAACCACAGG AGGATGGTAA GGCACAGACA 
CCACAAGGTC AAACACCATC ACAAAGTGGT AAAGAGTCGT CACAAAGTCA GAAATTGCGT 
GAGGCGTTCA TCCAATCTGC TGCAATAGAA ACTTTTTTCC TATGGGATAG ATATAAAAAG 
GTTAAAAATA AGGAAATAGC TGAAAAAAAA CAACGAGATA GTACAAACCC ATTTGGCTTC 
ACCCAGTCAA CATCAGGTGG AATGCAAGCA TTACCAGTTG GAGGAGCAGT ACAGGGAGCA 
CAAGCACAAC TACCCGATGG TGCCGGTGGT AGTGACCAAA CTCCCGAAAA ACAATTGGCT 
GGTGGCAAAA TACCTGAGGT TTTCTTACGT CAGATGTTTT ATACGTTAGG GGATTATAGA 
GATATATGTG TGGGGAACAC ACCTAGTGGT ATAGATACAA ATGATAAAGA AAATATGCAA 
AAAATACAAA ATAAAATAAA AAGTGTTATT GAAAAAAGTG ATTCCACACC TCGCACACCT 
GGTACCCACT CCCCATCTAG TGGTACAACC CCTCAAGCCT TGTGGGATAA AATCTCCCCT 
TCTATTTGGG AAGGAATGTT ATGTGCTTTA ACATACAAAG ATGGCGGAGA GGGCAAAAAA 
ATAGAAAAGG TTAACGATGC GAACGGCGAC GACCTTTTCC AAAAACTGAA AGACAAATAC 
AGCGACTACA AGACAGTCAC ACTCGAAGAA GATAATACCT CCTCCGCCAT GTCTACCAGT 
CCCAGAACCT CCGAAACCAC CTCAGCCTCT AGTGATAACA CCCCCACCCT CAACAACCCC 
AAATTGAGTG ATTTCGTGTT ACGCCCCCCC TACTTCCGAT ACCTTGAAGA ATGGGGTCAA 
AATTTTTGTA AAAAACGAAC AGAGATGTTG GAGAAGATAA AGGAGGAATG TCGCAGTGGC 
ACAGGAGGTC ATGAATATTG TAGTGGGGAT GGACATGATT GTACGGATAA TGATCGTAAA 
TATAATAAAA TGATTGCAGA TTTTCATTGT CCAGGTTGTG CAAAAGAATG TATGAAATAT 
AAAACATGGA TAGGAAAAAA ATTCGAAGAA TTTAAGAAGC AAGAAAATAC ATATAAAGTG 
GAACATGGTA AATTAACGAA TAATAATTGT AGTGGTAGTG ATAATACAAA ATTTTGTCAA 
CAAATAAAAA ATAATTCTTT TGATAAATTT TTGGAATTAT TGAGACATTG CAAAGATGGT 
AAGGATGATA CGGATAAAGA TAATGAATTA AATTTTAGTA AGCCTCTCCA AACCTTTTCT 
CGTTCAACGT ATTGTAAAGC GTGTCCTATA TATGGAGTTA AGTATAATAG GGGAACATAT 
AGCGCTATTA ATGAAAGTGA ATATATGAGC AAAAATGGTA TTAGTGGAGA AAATAAGAAC 
GATAAGAAAC CTACGGAAAT TAAAGTACTT CTGTTAGGTC GGAAAGGAGA AGTTAGGAAT 
AATGATGAAG AACTTAAAGA ACTTAAAGAA GTTTGTAATA ATGCCGGTTT TGTTGAGGAT 
TATAGTCTTC AAAAATGGAA CTGCCAAAAA AAGAATGGAG TGGATCAGTG TAATCTTACA 
AATTCTGTGG ATAATATAGA TGATAGTGAT AAAATTATTC CGTTTAATGT TTTTTTTCAG 
CGTTGGTTAA GAAATTTTGT ACACGATTAT AATAAATTGA AACAGAAAAT TCATCCATGT 
ATAAAAAATG AAGATGGAAA GGAACATAAA TGTATTGAAG GATGTAAAAA AAAATGTGAA 
TGTGTAGAAA AATGGTTAGA TAAAAAATCT ACAGAATGGG ATGAAATAAA AAAACATTAT 
GAGAAACATT CAAAAGATCA TAATGAAGGT ATTCCTCATA GTGTTAAAGG TTATCTTGTA 
CAGGCACCGT TTAAGGATGA AGTTCTCAAA GCTATAAAAC CTTGTGATTT TGATAACTTC 
AAGACTTCGT GTGGTCTTAA TGGCACTGAT AATTCACAAA ATGGTAATAA TAACGATCTT 
GTTTTATGTA TGATTAAAAA CCTTGAAAAA AAAATTGAAC AATGCAAAAA GAAACACGAC 
GAAAACAAAA AAACACCGTG TGATCCATTA CCCCCTCTAC CTGACGAAAA CGATGAAACC 
CTTGAAGAAA ACCCAGTGGC GCAACCGAAC ATTTGTCCGC AACTACCAGA GGAACCAAAA 
GAGACTTGTG AAGAAGCAGT CACTCCTAGT GAACCAAAAA AAGCGGAAGA AGAACCAGCA 
GCAGAACCCG AAGCCGTGCC CGAAGAAGAA GCACCGTTAC CTCCACCTCC CCCTGCGGCC 
CCACCTCGTG AGAGCAAAGA AAAAGCAAAA CCCCCCCCAA AACCTCGAAT CAAAACCCGT 
CATGTATTGG ACCACCCCGC TGTCATACCC GCCCTCATGT CTTCTACCAT CATGTGGAGT 
ATTGGCATCG GTTTTGCTGC GTTCACTTAT TTTTTTCTAA AGAAAAAAAC CAAATCATCT 
GTTGGAAATT TATTTCAAAT ACTGCAAATA CCCAAAAGTA ATTATGATAT ACCGACAAAA 
CGTTCACCCA ATAGATATAT ACCTTATACT AGTGGTAAAT ACAGAGGCAA ACGGTACATT 
TACCTTGAAG GAGATAGTGG AACAGATAGT GGTTACACCG ATCATTATAG TGATATTACT 
TCATCTTCCG AAAGTGAATA TGAAGAGATG GATATCAATG ATATATATGC ACCACGTGCT 
CCTAAATATA AAACGTTGAT AGAAGTGGTA CTTGAACCTA GTGGTAACAA CACAACAGCT 
AGTGGTAAAA ACACACCTAG TGATACACAA AATGATATAC CAACTAGTGA TACACCACCA 
CCCATTACTG ATGATGAGTG GAATACATTG AAACATGATT TTATATCTCA ATATTTACCA 
AATACACAAA ATACGGAACC AAATATTTTA CATGATAATT TGGATAATAA TACCCATCCC 
ACCCCGTCAC GTCATACGTT GGACCAAAAA CCTTTTATTA TGTCTATTCA TGATAGGGAT 
TTATATAGTG GAGAAGAAAT TAGTTATAAT ATTCATATGA GTACTAATAG TATGGATGAT 
CCAAAATATG TATCAAATAA TGTATATTCT GGTATCGATT TAATTAATGA CACATTAAGT 
GGTAACAAAC ATATTGATAT ATATGATGAA GTTTTGAAAA GAAAAGAAAA CGAATTATTT 
GGAACAAATT ATAAGAAAAA TACATCAAAC AATAGTGTTA CGAAAAATAT TAATAGTGAT 
CCTATAGATA ATCAGTTGAA TTTGTTTCAT ACATGGTTAG ATAGACATAG AGATATGTGT 
GAACAATGGA ATAATAAAGA GGAACTATTA GATAAATTGA AAGAAGAGTG GGAAAATGAG 
ACACATAGTG GTAACAAACA TAGTGACATA CCTAGTGGTA AACTAAGTGA TACACCTAGT 
GATAACAACA TACATAGTGA TATTCATCCT AGTGATATAC CTAGTGGTAA ACAAAGTGAT 
ATACCTAGTG ATAACAACAT ACATAGTGAC ATACCATACG TGTTAAATAC TGATGTTTCC 
ATACAAATAC ATATGGATAA TCCCAAAACT ACAAATGAGT TTACATATGT GGATAGTAAC 
CCCAACCAAG TGGATGACAC CTACGTGGAT AGTAACCCTG ACAATTCTTC CATGGATACT 
ATCTTGGACG ATCTGGAAAA ATATAATGAA CCTTATTATG ATGTACAAGA TGATATTTAT 
TATGATGTAA ATGATCATGA TACATCAACT GTGGATAGTA ATGCTATGGA TATACCTAGC 
AAAGTACAAA TTGAAATGGA TGTAAATACC AAATTGGTGA AAGAGAAATA TCCTATAGGA 
GATGTGTGGG ATATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PF3D7_0808600 cDNA ORF clone, Plasmodium falciparum 3D7

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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