PBANKA_081860 cDNA ORF clone, Plasmodium berghei ANKA

Gene
Clones
gRNAs

Summary

Gene Symbol	PBANKA_081860
Entrez Gene ID	3427856
Full Name	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43, putative
Synonyms	PB000897.02.0
Gene Type	protein-coding
Organism	Plasmodium berghei ANKA

mRNA and Protein(s)

mRNA	Protein	Name
XM_674162.1	XP_679254.1	ATP-dependant RNA helicase
XM_674162.2	XP_679254.2	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43, putative

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A comprehensive evaluation of rodent malaria parasite genomes and gene expression.
Otto TD, Böhme U, Jackson AP, Hunt M, Franke-Fayard B, Hoeijmakers WA, Religa AA, Robertson L, Sanders M, Ogun SA, Cunningham D, Erhart A, Billker O, Khan SM, Stunnenberg HG, Langhorne J, Holder AA, Waters AP, Newbold CI, Pain A, Berriman M, Janse CJ
BMC biology1286(2014 Oct)

A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses.
Hall N, Karras M, Raine JD, Carlton JM, Kooij TW, Berriman M, Florens L, Janssen CS, Pain A, Christophides GK, James K, Rutherford K, Harris B, Harris D, Churcher C, Quail MA, Ormond D, Doggett J, Trueman HE, Mendoza J, Bidwell SL, Rajandream MA, Carucci DJ, Yates JR 3rd, Kafatos FC, Janse CJ, Barrell B, Turner CM, Waters AP, Sinden RE
Science (New York, N.Y.)307(5706)82-6(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PBANKA_081860 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PBANKA_081860 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPm00398	XM_674162.1 Latest version!	Plasmodium berghei strain ANKA ATP-dependant RNA helicase, (PB000897.02.0) partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OPm05155	XM_674162.2 Latest version!	Plasmodium berghei ANKA pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43, putative (PBANKA_081860), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PBANKA_081860 ( XM_674162.1 ) cDNA ORF clone, Plasmodium berghei ANKA -> XP_679254.1 Plasmodium berghei strain ANKA ATP-dependant RNA helicase, (PB000897.02.0) partial mRNA.

Price & Availability↑

CloneID	OPm00398
Clone ID Related Accession (Same CDS sequence)	XM_674162.1
Accession Version	XM_674162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1145462400000
Organism	Plasmodium berghei ANKA
Product	ATP-dependant RNA helicase
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_674714). COMPLETENESS: not full length.

ATGGGTTCTA TAAAAAATTT CGTAAATAAA GAAATTATTA ATGATAAAAA TATATCTGAA 
AAAGATGCAG ATGAAAATAA CCTTATAAAT AAATTAACAA ATAAAAGATA TAGTGATAGA 
TATTTAGAAT TACTTGAATC CAAAAAAAAA TTGCCTGCAT GGGCAGCTAA AAAAAACTTT 
TTAAAATTAT TTAAAAAAAA TAATGTAATA ATAATTGTTG GGGATACAGG AAGTGGAAAA 
ACAACACAAA TATCTCAGTT TGTTTTAGAA TCTAAATTTT CAGAAAAAAA ATCTATAGCA 
GTTACTCAGC CAAGGAGAGT TGCAGCAATG AGTGTGGCTG CACGCGTAGC AGAGGAATTA 
GATGTAGAAC TAGGAACATA TGTAGGATAT ACAATAAGAT TTGAAGATAA ATCTTGTCAT 
AAAACAATTA TAAAATATTT AACAGATGGA ATGCTTTTAA GAGAATCTAT GTTTGATCCT 
TTATTAAAAA GATATAATGT AATAATTTTA GATGAAGCAC ATGAAAGAAC ATTATCTACA 
GATATATTAT TTGGAGTAAT CAAAAATATT CAAGAAAAAA GAGATGATTT GAAATTAGTT 
GTTATGTCTG CTACTTTAGA TGCAGAAAAA TTCCAAAATT TTTTTAACAA TTCTAAGATA 
TTAAATATTC CAGGTCGATT ATATCCCGTT GAAATATTTT ATACAATGCA CCCAGAAAAA 
TGTTATATAA AAGTTGTAAT TAAAACTGTA TATAATATAC ATACTAATGA AGAAGAGGGT 
GATATATTAG TATTTTTAAC AGGAGAAGAT GAAATTGAAA TGACGAAAAA GGAAATAGAA 
AAATTAGTAT CTAAAAAACC TGGTATACCT CAATTAGTAT GTTTACCATT ATATTCTTCA 
TTGCCACCAG CACAACAACA AAAGATTTTT GAACCTGCCC CACCACCACG ATATAAAGGA 
GATAAAAAAG GAAGAAAATG TATATTAGCA ACTAATATTG CTGAAACATC TATAACTATT 
GATGGAATTG TTTATGTAAT TGATCCTGGC TTTTCAAAAC AAAAAGTTTA TAACCCAAGA 
GCAAGAATAG AATCATTACT TATAGCCCCT ATTTCTAAAG CATCAGCCCA ACAAAGAGCT 
GGAAGGGCTG GTAGAACAAA GCCAGGAAAA TGTTTTCGTT TGTATACAGA AAAATGTTTT 
AATGAAACTT TACCTGAACA AACATATCCA GAAATTTTAA GATCAAATCT AGGATCTGTT 
GTATTAAATT TAAAAAAATT AGGTATTGAC GATTTAGTAC ATTTCGATTT TATGGATCCT 
CCTGCCCCTG AAACTTTAAT GAGAGCCTTA GAACAATTAA ATTATTTAGA GGCATTAGAT 
GATGAAGGAG AGTTAACAAA AAAAGGACAT ATAATGAGTG AGTTTCCTGT AGACCCACAG 
CTAGCTAAAG TATTATTAGA ATCATCTAAT TATTCATGCT CTAGCGAAAT ATTATCTATT 
GCTGCTATGC TATCTGTTCC ACAATGTTTT TTGAGACCAA AAATAAAGGG AAAAGAAGCT 
GATGAAATGA AAGCTCGATT TTCACATCTG GATGGAGATC ATTTAACATT GCTTAATGTT 
TTTCATGCAT TTGTTAAGCA TTCATTAGTT GATCAAAATG AATCTAGAAA ATTTTGTTAT 
GATCATTTTT TAAATCATAG AACTATGACA TCTGCTCAAA ATGTTAGATT ACAATTATTA 
AAAACTATGG AAAGATTGGG ATTAAAAATT ACATCTATCA ATCCATCGAA CCCAAATTAT 
TATATCAATA TTAGAAAAGC ACTATTAAGT GGGTTCTATC AACAAGTAGC TTATAAAACA 
AACAAAGGAT ATTATATTAC AGTAAAGGAT ATACAAATTG TTACTTTACA CCCATCTACT 
GTTTTTCAAA TTAATCCAGA ATGGGTTCTA TATCATGAAC TTATATTAAC ATCAAAAAAT 
TTTATAAGAA CCGTTACAAA AATAGATGGA TCTTGGCTTC TCGAGGTTGC AAAAAATTAT 
TACACCTTAG AAGATCTACC TAATAGTGAA GCCAAAAATG AACTTAAATT GATGGCAAAA 
TCAATTAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_679254.1
CDS	1..2112
Translation	MGSIKNFVNKEIINDKNISEKDADENNLINKLTNKRYSDRYLELLESKKKLPAWAAKKNFLKLFKKNNVIIIVGDTGSGKTTQISQFVLESKFSEKKSIAVTQPRRVAAMSVAARVAEELDVELGTYVGYTIRFEDKSCHKTIIKYLTDGMLLRESMFDPLLKRYNVIILDEAHERTLSTDILFGVIKNIQEKRDDLKLVVMSATLDAEKFQNFFNNSKILNIPGRLYPVEIFYTMHPEKCYIKVVIKTVYNIHTNEEEGDILVFLTGEDEIEMTKKEIEKLVSKKPGIPQLVCLPLYSSLPPAQQQKIFEPAPPPRYKGDKKGRKCILATNIAETSITIDGIVYVIDPGFSKQKVYNPRARIESLLIAPISKASAQQRAGRAGRTKPGKCFRLYTEKCFNETLPEQTYPEILRSNLGSVVLNLKKLGIDDLVHFDFMDPPAPETLMRALEQLNYLEALDDEGELTKKGHIMSEFPVDPQLAKVLLESSNYSCSSEILSIAAMLSVPQCFLRPKIKGKEADEMKARFSHLDGDHLTLLNVFHAFVKHSLVDQNESRKFCYDHFLNHRTMTSAQNVRLQLLKTMERLGLKITSINPSNPNYYINIRKALLSGFYQQVAYKTNKGYYITVKDIQIVTLHPSTVFQINPEWVLYHELILTSKNFIRTVTKIDGSWLLEVAKNYYTLEDLPNSEAKNELKLMAKSIR

Target ORF information:

RefSeq Version	XM_674162.1
Organism	Plasmodium berghei ANKA
Definition	Plasmodium berghei strain ANKA ATP-dependant RNA helicase, (PB000897.02.0) partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_674162.1

ORF Insert Sequence:

ATGGGTTCTA TAAAAAATTT CGTAAATAAA GAAATTATTA ATGATAAAAA TATATCTGAA 
AAAGATGCAG ATGAAAATAA CCTTATAAAT AAATTAACAA ATAAAAGATA TAGTGATAGA 
TATTTAGAAT TACTTGAATC CAAAAAAAAA TTGCCTGCAT GGGCAGCTAA AAAAAACTTT 
TTAAAATTAT TTAAAAAAAA TAATGTAATA ATAATTGTTG GGGATACAGG AAGTGGAAAA 
ACAACACAAA TATCTCAGTT TGTTTTAGAA TCTAAATTTT CAGAAAAAAA ATCTATAGCA 
GTTACTCAGC CAAGGAGAGT TGCAGCAATG AGTGTGGCTG CACGCGTAGC AGAGGAATTA 
GATGTAGAAC TAGGAACATA TGTAGGATAT ACAATAAGAT TTGAAGATAA ATCTTGTCAT 
AAAACAATTA TAAAATATTT AACAGATGGA ATGCTTTTAA GAGAATCTAT GTTTGATCCT 
TTATTAAAAA GATATAATGT AATAATTTTA GATGAAGCAC ATGAAAGAAC ATTATCTACA 
GATATATTAT TTGGAGTAAT CAAAAATATT CAAGAAAAAA GAGATGATTT GAAATTAGTT 
GTTATGTCTG CTACTTTAGA TGCAGAAAAA TTCCAAAATT TTTTTAACAA TTCTAAGATA 
TTAAATATTC CAGGTCGATT ATATCCCGTT GAAATATTTT ATACAATGCA CCCAGAAAAA 
TGTTATATAA AAGTTGTAAT TAAAACTGTA TATAATATAC ATACTAATGA AGAAGAGGGT 
GATATATTAG TATTTTTAAC AGGAGAAGAT GAAATTGAAA TGACGAAAAA GGAAATAGAA 
AAATTAGTAT CTAAAAAACC TGGTATACCT CAATTAGTAT GTTTACCATT ATATTCTTCA 
TTGCCACCAG CACAACAACA AAAGATTTTT GAACCTGCCC CACCACCACG ATATAAAGGA 
GATAAAAAAG GAAGAAAATG TATATTAGCA ACTAATATTG CTGAAACATC TATAACTATT 
GATGGAATTG TTTATGTAAT TGATCCTGGC TTTTCAAAAC AAAAAGTTTA TAACCCAAGA 
GCAAGAATAG AATCATTACT TATAGCCCCT ATTTCTAAAG CATCAGCCCA ACAAAGAGCT 
GGAAGGGCTG GTAGAACAAA GCCAGGAAAA TGTTTTCGTT TGTATACAGA AAAATGTTTT 
AATGAAACTT TACCTGAACA AACATATCCA GAAATTTTAA GATCAAATCT AGGATCTGTT 
GTATTAAATT TAAAAAAATT AGGTATTGAC GATTTAGTAC ATTTCGATTT TATGGATCCT 
CCTGCCCCTG AAACTTTAAT GAGAGCCTTA GAACAATTAA ATTATTTAGA GGCATTAGAT 
GATGAAGGAG AGTTAACAAA AAAAGGACAT ATAATGAGTG AGTTTCCTGT AGACCCACAG 
CTAGCTAAAG TATTATTAGA ATCATCTAAT TATTCATGCT CTAGCGAAAT ATTATCTATT 
GCTGCTATGC TATCTGTTCC ACAATGTTTT TTGAGACCAA AAATAAAGGG AAAAGAAGCT 
GATGAAATGA AAGCTCGATT TTCACATCTG GATGGAGATC ATTTAACATT GCTTAATGTT 
TTTCATGCAT TTGTTAAGCA TTCATTAGTT GATCAAAATG AATCTAGAAA ATTTTGTTAT 
GATCATTTTT TAAATCATAG AACTATGACA TCTGCTCAAA ATGTTAGATT ACAATTATTA 
AAAACTATGG AAAGATTGGG ATTAAAAATT ACATCTATCA ATCCATCGAA CCCAAATTAT 
TATATCAATA TTAGAAAAGC ACTATTAAGT GGGTTCTATC AACAAGTAGC TTATAAAACA 
AACAAAGGAT ATTATATTAC AGTAAAGGAT ATACAAATTG TTACTTTACA CCCATCTACT 
GTTTTTCAAA TTAATCCAGA ATGGGTTCTA TATCATGAAC TTATATTAAC ATCAAAAAAT 
TTTATAAGAA CCGTTACAAA AATAGATGGA TCTTGGCTTC TCGAGGTTGC AAAAAATTAT 
TACACCTTAG AAGATCTACC TAATAGTGAA GCCAAAAATG AACTTAAATT GATGGCAAAA 
TCAATTAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBANKA_081860 ( XM_674162.2 ) cDNA ORF clone, Plasmodium berghei ANKA -> XP_679254.2 Plasmodium berghei ANKA pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43, putative (PBANKA_081860), partial mRNA.

Price & Availability↑

CloneID	OPm05155
Clone ID Related Accession (Same CDS sequence)	XM_674162.2
Accession Version	XM_674162.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1508688000000
Organism	Plasmodium berghei ANKA
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_036166). On Oct 24, 2017 this sequence version replaced XM_674162.1. COMPLETENESS: incomplete on both ends.

ATGGAAAATA TATCTGAATG TATAGAACAA AAAAGTTCTG AAGAAAATGA TGAAACAAAA 
CAGAATCAAG AAATAACTCA TAAAAGAAAA AAAGAAGAAT CTCAAGCTGA TGAGAAAGAA 
AATATTGATA TGAACAGTAA TATAAAAAGG CATAAAATAA TTGATTTATA TAATAATGAA 
GAATGTAAAA ATAGTGTATC TAATAATAAT AATTTTAAAG AAAACGAAGA ACTTAATATG 
GGTTCTATAA AAAATTTCGT AAATAAAGAA ATTATTAATG ATAAAAATAT ATCTGAAAAA 
GATGCAGATG AAAATAACCT TATAAATAAA TTAACAAATA AAAGATATAG TGATAGATAT 
TTAGAATTAC TTGAATCCAA AAAAAAATTG CCTGCATGGG CAGCTAAAAA AAACTTTTTA 
AAATTATTTA AAAAAAATAA TGTAATAATA ATTGTTGGGG ATACAGGAAG TGGAAAAACA 
ACACAAATAT CTCAGTTTGT TTTAGAATCT AAATTTTCAG AAAAAAAATC TATAGCAGTT 
ACTCAGCCAA GGAGAGTTGC AGCAATGAGT GTGGCTGCAC GCGTAGCAGA GGAATTAGAT 
GTAGAACTAG GAACATATGT AGGATATACA ATAAGATTTG AAGATAAATC TTGTCATAAA 
ACAATTATAA AATATTTAAC AGATGGAATG CTTTTAAGAG AATCTATGTT TGATCCTTTA 
TTAAAAAGAT ATAATGTAAT AATTTTAGAT GAAGCACATG AAAGAACATT ATCTACAGAT 
ATATTATTTG GAGTAATCAA AAATATTCAA GAAAAAAGAG ATGATTTGAA ATTAGTTGTT 
ATGTCTGCTA CTTTAGATGC AGAAAAATTC CAAAATTTTT TTAACAATTC TAAGATATTA 
AATATTCCAG GTCGATTATA TCCCGTTGAA ATATTTTATA CAATGCACCC AGAAAAATGT 
TATATAAAAG TTGTAATTAA AACTGTATAT AATATACATA CTAATGAAGA AGAGGGTGAT 
ATATTAGTAT TTTTAACAGG AGAAGATGAA ATTGAAATGA CGAAAAAGGA AATAGAAAAA 
TTAGTATCTA AAAAACCTGG TATACCTCAA TTAGTATGTT TACCATTATA TTCTTCATTG 
CCACCAGCAC AACAACAAAA GATTTTTGAA CCTGCCCCAC CACCACGATA TAAAGGAGAT 
AAAAAAGGAA GAAAATGTAT ATTAGCAACT AATATTGCTG AAACATCTAT AACTATTGAT 
GGAATTGTTT ATGTAATTGA TCCTGGCTTT TCAAAACAAA AAGTTTATAA CCCAAGAGCA 
AGAATAGAAT CATTACTTAT AGCCCCTATT TCTAAAGCAT CAGCCCAACA AAGAGCTGGA 
AGGGCTGGTA GAACAAAGCC AGGAAAATGT TTTCGTTTGT ATACAGAAAA ATGTTTTAAT 
GAAACTTTAC CTGAACAAAC ATATCCAGAA ATTTTAAGAT CAAATCTAGG ATCTGTTGTA 
TTAAATTTAA AAAAATTAGG TATTGACGAT TTAGTACATT TCGATTTTAT GGATCCTCCT 
GCCCCTGAAA CTTTAATGAG AGCCTTAGAA CAATTAAATT ATTTAGAGGC ATTAGATGAT 
GAAGGAGAGT TAACAAAAAA AGGACATATA ATGAGTGAGT TTCCTGTAGA CCCACAGCTA 
GCTAAAGTAT TATTAGAATC ATCTAATTAT TCATGCTCTA GCGAAATATT ATCTATTGCT 
GCTATGCTAT CTGTTCCACA ATGTTTTTTG AGACCAAAAA TAAAGGGAAA AGAAGCTGAT 
GAAATGAAAG CTCGATTTTC ACATCTGGAT GGAGATCATT TAACATTGCT TAATGTTTTT 
CATGCATTTG TTAAGCATTC ATTAGTTGAT CAAAATGAAT CTAGAAAATT TTGTTATGAT 
CATTTTTTAA ATCATAGAAC TATGACATCT GCTCAAAATG TTAGATTACA ATTATTAAAA 
ACTATGGAAA GATTGGGATT AAAAATTACA TCTATCAATC CATCGAACCC AAATTATTAT 
ATCAATATTA GAAAAGCACT ATTAAGTGGG TTCTATCAAC AAGTAGCTTA TAAAACAAAC 
AAAGGATATT ATATTACAGT AAAGGATATA CAAATTGTTA CTTTACACCC ATCTACTGTT 
TTTCAAATTA ATCCAGAATG GGTTCTATAT CATGAACTTA TATTAACATC AAAAAATTTT 
ATAAGAACCG TTACAAAAAT AGATGGATCT TGGCTTCTCG AGGTTGCAAA AAATTATTAC 
ACCTTAGAAG ATCTACCTAA TAGTGAAGCC AAAAATGAAC TTAAATTGAT GGCAAAATCA 
ATTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_679254.2
CDS	1..2349
Translation	MENISECIEQKSSEENDETKQNQEITHKRKKEESQADEKENIDMNSNIKRHKIIDLYNNEECKNSVSNNNNFKENEELNMGSIKNFVNKEIINDKNISEKDADENNLINKLTNKRYSDRYLELLESKKKLPAWAAKKNFLKLFKKNNVIIIVGDTGSGKTTQISQFVLESKFSEKKSIAVTQPRRVAAMSVAARVAEELDVELGTYVGYTIRFEDKSCHKTIIKYLTDGMLLRESMFDPLLKRYNVIILDEAHERTLSTDILFGVIKNIQEKRDDLKLVVMSATLDAEKFQNFFNNSKILNIPGRLYPVEIFYTMHPEKCYIKVVIKTVYNIHTNEEEGDILVFLTGEDEIEMTKKEIEKLVSKKPGIPQLVCLPLYSSLPPAQQQKIFEPAPPPRYKGDKKGRKCILATNIAETSITIDGIVYVIDPGFSKQKVYNPRARIESLLIAPISKASAQQRAGRAGRTKPGKCFRLYTEKCFNETLPEQTYPEILRSNLGSVVLNLKKLGIDDLVHFDFMDPPAPETLMRALEQLNYLEALDDEGELTKKGHIMSEFPVDPQLAKVLLESSNYSCSSEILSIAAMLSVPQCFLRPKIKGKEADEMKARFSHLDGDHLTLLNVFHAFVKHSLVDQNESRKFCYDHFLNHRTMTSAQNVRLQLLKTMERLGLKITSINPSNPNYYINIRKALLSGFYQQVAYKTNKGYYITVKDIQIVTLHPSTVFQINPEWVLYHELILTSKNFIRTVTKIDGSWLLEVAKNYYTLEDLPNSEAKNELKLMAKSIR

Target ORF information:

RefSeq Version	XM_674162.2
Organism	Plasmodium berghei ANKA
Definition	Plasmodium berghei ANKA pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43, putative (PBANKA_081860), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_674162.2

ORF Insert Sequence:

ATGGAAAATA TATCTGAATG TATAGAACAA AAAAGTTCTG AAGAAAATGA TGAAACAAAA 
CAGAATCAAG AAATAACTCA TAAAAGAAAA AAAGAAGAAT CTCAAGCTGA TGAGAAAGAA 
AATATTGATA TGAACAGTAA TATAAAAAGG CATAAAATAA TTGATTTATA TAATAATGAA 
GAATGTAAAA ATAGTGTATC TAATAATAAT AATTTTAAAG AAAACGAAGA ACTTAATATG 
GGTTCTATAA AAAATTTCGT AAATAAAGAA ATTATTAATG ATAAAAATAT ATCTGAAAAA 
GATGCAGATG AAAATAACCT TATAAATAAA TTAACAAATA AAAGATATAG TGATAGATAT 
TTAGAATTAC TTGAATCCAA AAAAAAATTG CCTGCATGGG CAGCTAAAAA AAACTTTTTA 
AAATTATTTA AAAAAAATAA TGTAATAATA ATTGTTGGGG ATACAGGAAG TGGAAAAACA 
ACACAAATAT CTCAGTTTGT TTTAGAATCT AAATTTTCAG AAAAAAAATC TATAGCAGTT 
ACTCAGCCAA GGAGAGTTGC AGCAATGAGT GTGGCTGCAC GCGTAGCAGA GGAATTAGAT 
GTAGAACTAG GAACATATGT AGGATATACA ATAAGATTTG AAGATAAATC TTGTCATAAA 
ACAATTATAA AATATTTAAC AGATGGAATG CTTTTAAGAG AATCTATGTT TGATCCTTTA 
TTAAAAAGAT ATAATGTAAT AATTTTAGAT GAAGCACATG AAAGAACATT ATCTACAGAT 
ATATTATTTG GAGTAATCAA AAATATTCAA GAAAAAAGAG ATGATTTGAA ATTAGTTGTT 
ATGTCTGCTA CTTTAGATGC AGAAAAATTC CAAAATTTTT TTAACAATTC TAAGATATTA 
AATATTCCAG GTCGATTATA TCCCGTTGAA ATATTTTATA CAATGCACCC AGAAAAATGT 
TATATAAAAG TTGTAATTAA AACTGTATAT AATATACATA CTAATGAAGA AGAGGGTGAT 
ATATTAGTAT TTTTAACAGG AGAAGATGAA ATTGAAATGA CGAAAAAGGA AATAGAAAAA 
TTAGTATCTA AAAAACCTGG TATACCTCAA TTAGTATGTT TACCATTATA TTCTTCATTG 
CCACCAGCAC AACAACAAAA GATTTTTGAA CCTGCCCCAC CACCACGATA TAAAGGAGAT 
AAAAAAGGAA GAAAATGTAT ATTAGCAACT AATATTGCTG AAACATCTAT AACTATTGAT 
GGAATTGTTT ATGTAATTGA TCCTGGCTTT TCAAAACAAA AAGTTTATAA CCCAAGAGCA 
AGAATAGAAT CATTACTTAT AGCCCCTATT TCTAAAGCAT CAGCCCAACA AAGAGCTGGA 
AGGGCTGGTA GAACAAAGCC AGGAAAATGT TTTCGTTTGT ATACAGAAAA ATGTTTTAAT 
GAAACTTTAC CTGAACAAAC ATATCCAGAA ATTTTAAGAT CAAATCTAGG ATCTGTTGTA 
TTAAATTTAA AAAAATTAGG TATTGACGAT TTAGTACATT TCGATTTTAT GGATCCTCCT 
GCCCCTGAAA CTTTAATGAG AGCCTTAGAA CAATTAAATT ATTTAGAGGC ATTAGATGAT 
GAAGGAGAGT TAACAAAAAA AGGACATATA ATGAGTGAGT TTCCTGTAGA CCCACAGCTA 
GCTAAAGTAT TATTAGAATC ATCTAATTAT TCATGCTCTA GCGAAATATT ATCTATTGCT 
GCTATGCTAT CTGTTCCACA ATGTTTTTTG AGACCAAAAA TAAAGGGAAA AGAAGCTGAT 
GAAATGAAAG CTCGATTTTC ACATCTGGAT GGAGATCATT TAACATTGCT TAATGTTTTT 
CATGCATTTG TTAAGCATTC ATTAGTTGAT CAAAATGAAT CTAGAAAATT TTGTTATGAT 
CATTTTTTAA ATCATAGAAC TATGACATCT GCTCAAAATG TTAGATTACA ATTATTAAAA 
ACTATGGAAA GATTGGGATT AAAAATTACA TCTATCAATC CATCGAACCC AAATTATTAT 
ATCAATATTA GAAAAGCACT ATTAAGTGGG TTCTATCAAC AAGTAGCTTA TAAAACAAAC 
AAAGGATATT ATATTACAGT AAAGGATATA CAAATTGTTA CTTTACACCC ATCTACTGTT 
TTTCAAATTA ATCCAGAATG GGTTCTATAT CATGAACTTA TATTAACATC AAAAAATTTT 
ATAAGAACCG TTACAAAAAT AGATGGATCT TGGCTTCTCG AGGTTGCAAA AAATTATTAC 
ACCTTAGAAG ATCTACCTAA TAGTGAAGCC AAAAATGAAC TTAAATTGAT GGCAAAATCA 
ATTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A comprehensive evaluation of rodent malaria parasite genomes and gene expression.
BMC biology1286(2014 Oct)
Otto TD,Böhme U,Jackson AP,Hunt M,Franke-Fayard B,Hoeijmakers WA,Religa AA,Robertson L,Sanders M,Ogun SA,Cunningham D,Erhart A,Billker O,Khan SM,Stunnenberg HG,Langhorne J,Holder AA,Waters AP,Newbold CI,Pain A,Berriman M,Janse CJ

A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses.
Science (New York, N.Y.)307(5706)82-6(2005 Jan)
Hall N,Karras M,Raine JD,Carlton JM,Kooij TW,Berriman M,Florens L,Janssen CS,Pain A,Christophides GK,James K,Rutherford K,Harris B,Harris D,Churcher C,Quail MA,Ormond D,Doggett J,Trueman HE,Mendoza J,Bidwell SL,Rajandream MA,Carucci DJ,Yates JR 3rd,Kafatos FC,Janse CJ,Barrell B,Turner CM,Waters AP,Sinden RE

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PBANKA_081860 cDNA ORF clone, Plasmodium berghei ANKA

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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