F5 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	F5
Entrez Gene ID	102975793
Full Name	coagulation factor V
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024116689.1	XP_023972457.1	coagulation factor V isoform X1
XM_024116690.1	XP_023972458.1	coagulation factor V isoform X2
XM_028488690.1	XP_028344491.1	coagulation factor V isoform X1
XM_028488691.1	XP_028344492.1	coagulation factor V isoform X2
XM_007102057.1	XP_007102119.1	coagulation factor V

Pathways from BioSystems
Gene Ontology

The following F5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh328360	XM_028488691.1 Latest version!	Physeter catodon coagulation factor V (F5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh112271	XM_007102057.1 Latest version!	Physeter catodon coagulation factor V (proaccelerin, labile factor) (F5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPh305667	XM_024116689.1 Latest version!	Physeter catodon coagulation factor V (F5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPh305668	XM_024116690.1 Latest version!	Physeter catodon coagulation factor V (F5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPh328359	XM_028488690.1 Latest version!	Physeter catodon coagulation factor V (F5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F5 ( XM_028488691.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_028344492.1 Physeter catodon coagulation factor V (F5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPh328360
Clone ID Related Accession (Same CDS sequence)	XM_028488691.1
Accession Version	XM_028488691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	coagulation factor V isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041217.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAG CTTGCCCTTG CCTCTGGGTC CTCGTGGTCC TGGGCTCCAG CTGGGCAGGC 
TGGGGGAGCC TAGGGGCTGA AGCAGCGCGG CTAAGGCAGT TCTACGTGGC TGCTCAGAGC 
ATCAGCTGGA ACTATCTCCC CGAGTCCACA AACCTTAGTT CGAATACTTT TGCAACCTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACGATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAGGCAGA CAAACCCTTA AGCATCCATC CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCTTC TTACCCTGAC CACACATTCC CTGTGGAGAG GATGGATGAT 
TCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGAATA TCAGTGAGGA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACATAT ATCTATTACT CCTATGAAAA TCTGGTACAC 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACTGAG 
GATGGGATAC AGAAGATGTT TGACAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCCTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGTCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATTCAT TTCAATGGCC AGGTCCTGGA AGAGAACCAT 
CATAAGATGT CAGCCATCAC TCTCGTCAGT GCTACGTCCA CAACTGCAAA CATGACTGTG 
AGCCTAGAGG GAAAGTGGAT CATATCTTCG CTCATCCCAA AACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GGAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AGTACAGATC TCTGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGCATCCT TCACCAAACG CCTGGAGAAT CCCAATGTCA AAGAAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTTAAAATCA TGTTCAAAAA TATGGCCAGC 
CGCAGCTACA GCATTTACCC TCATGGTGTG ACCTTCTCTC CTTATGAAGA TGAAGTCAAC 
TCTTCCTTCG CCTCAGGCAG TAACACCATG ATCAAAGCAG TTCAACCAGG GGAAACCTAT 
ACTTATAAGT GGAACATCCT AGAGTCTGAT GAACCCATGG AAAATGATGC TCAATGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACA TTGCCTCTGG GCTGATAGGG 
CTAGTTCTAA TTTGTAAGAG CAGATCCTTG GATAAGCGAG GCATACAGAG GACAGCAGAC 
ATCGAGCAAC AGGCTGTGTT TGCTGTGTTT GATGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAGGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGCAC TATCAATGGC TATGTGCCTG AGAGCATACC CACACTAGGA 
TTCTGCTTTG ATGACACCGT CCAGTGGCAC TTCTGCAGTA TGGGAACCCA GAATGACATT 
TTGACCATTC ACTTCACTGG GCACTCATTC ATCTATGGAA AGAAACATGA GGACACCTTG 
ACCCTCTTCC CCATGCGTGG GGAATCTGTG ACCGTCACAA TGGATAACGT TGGAACTTGG 
ATGTTAACTA CCATGAATTC CAATCCAAGA AGCAAAAAAC TGTGGCTGAG ATTCAGGGAT 
GTTAAGTGTA TCCGGGAGTA TGATGATGAC TCATATGAGA TTATATACGA ACCTTCAGGA 
TCTACATCCA AAACTACAAG GAAAATGCGT GATTCTTCAG AAATCAAAGA TAAAACTAAT 
GATGCTGACT ATGATTACCA GAACACACTG GCTTTATTAT TAGGAATCAG GTCATTCAGA 
AATTCATCAT CAAATCAGGA GGAAGATGAG TTCAATCTTA CTGCCCTGGC TCTGGGGAAC 
AACTCTGAAT TCATTCCTCC AAGTACCAAC ACAACTGTTG GTTCAAATTC TTCTTCCCCA 
GGTAACATTA GCAGGCTTAT TGCCAATAAC TTTGCACAAC TTTTGAAAAT TCTTCCTCAC 
TCAGAAGCCA CCACAGCTGG TCCCCCACTG GGACACACCA GCTTAGATAA GAACTCAGTT 
CTCAACCCTC CCACAGCAGA GCATTCCAGC CCTTATTCTG AAGATCCTCT ATACTCAGAT 
GTCACAGGGA TAAGCCTGCT TCCATTTGCT ACAGAAGAAT TCAGAAATCA AGAACATGCT 
AAACGTAAAA GATTCAAGGC AGGAAGAGGC CGAGCAGCAA AGCAAAGGTT CTCCCAGATG 
GAATTCCTAG CACGTAAAAC TGGTAGACAT TTAAGCAAAG GCAACTCTTC TTCTTCCAGA 
ATAGGGCCCT GGGAGAACCT TCCCAGTGAT CTGTTACTCT TGCAACAAAA GGATCCATCT 
AAGATTCTGA ATGGGGAATG GCATTTGGTT TCTGAGAAAG GCAGTTATGA AATAATCCAA 
GATGCTGATG AAGACAAGGC TGTTAATAAG CTGCCAAACA GCCCCCAGAA TGACTCAAGG 
ACTTGGGGAG AAAACATCCC TTTTAAAAAC AAGCATGGAA AGCAGAGTGG CCACCCAACG 
TTTTTTGTAA TTAGACATAA ACCTCTACAA GGAAGACAAG ATGGAGGAAA TAGTAGATTG 
AAGAAAGGCC TTTTTTTAAT TAGGACACGA AGAAAGAAAA AGGAAGAGAA GCCTGCACAC 
CATGTTCCTC TATCTCCAAG GAGTTTTTAC CCTCTAAGAG TAGAGGCCAA CACCCCATCT 
TCAGATGGGA GACATAATCA TTCATTGTTA CTCCATACAT CCAATGAAAC ATCTCTTCCC 
ACAGACCTCA ATCAGACATT CCCCTCTATG AACCTTAGCC TCATAGCCTC ACTTCCTGAT 
GGTGATCAAA ACCCCCCAAA TGACACCATT CAGACAAGCT CCCCTCCAGA TCTTTATCAG 
ACAGTGAGCC CAGAGGAACA CTATCAAACA TTCCCTATTC AAGACTCTGA TCCAACGCAC 
TCCACTACAG TCCCCAGTCA CAGATATCCT CCTCCAGAGC CCAGCCAGAT ACCCGACTAT 
GACCTAAGAA ACAAGGCCTT TCCTACTGAT GTTAGTCAAA CGTTCCCTTC CTTGGAACTT 
GAAGTCTGGC AGACAACCAC CTCTCTAGAC CTCAGTCAGC CATCCCTCTA TCCAGATCTC 
AGCCAGACCA CCCTTTCTCC AGACCCTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT TGGCCAGGAG ACTCTCTCTC CAGACCTCAG CCAGACAACA 
CTTTCCCCAG ACCTTGGCCA GGAGACTCTC TCTCCAGACC TCAGCCAGAC AACACTTTCC 
CCAGTTCTTA GCCAGGAGAC TCTCTCTCCA GACCTCAGCC AGATGGCCCT TTCCCCACAC 
CCTGGCCAGG AGACGCTTTC TCCAGACTTC AGCCAGACAA CACTTTCCCC AGACCTTAGT 
CAGGAGACTC TCTCTCCAGA CCTCGGACAG ATAGCCCTTT CCCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACA TTTTCCCCAG ACTTCAGCCA GACTATCTCT 
CCAGACCTCA GACAGGAGAC TCTCTCTCCA GGCCTCAACC AGACAGCCCT TTCCCCAGAC 
CTTAGCCAGG AGACTCTCTC TCCAGACCTC AGCCAGACAA CATTTTCCCC AGACCTTAGC 
CAGGAGACTC TCTCTCCAGA CCTCAGCCCG ATGGCCCTTT CGCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACG CTTTCCCCAG ACCTCAGCCA GGCTCTCTCT 
CCAGACATCA GACAGATGGT CCTTTCCCCA GACCTCAGCC AGGAGATTCT CTCTCCAGAC 
CTCAGCCAGA CAACACCTTC CCCAGACCTT GGCCAGGAGA CTCTCTCTGC AGACCTCAGC 
CAGACAACAC TTTCCCCAGA CCTTGGCCAG GAGACTCTCT CTGCAGACCT CAGCCAGACA 
ACACTTTCCC CAGACCTCGG CCAGGAGACT CTCTCTCCAG ACCTCAGCCA GACAACACTT 
TCCCCAGACC TTGGCCAGGA GACTCTCTCT CCAGACCTCA GCCAGACAAC ACTTTCCCCA 
GACCTCAGCC AGGCTCTCTC TCCAGACATC AGCCAGATGG TCCTTTCCCC AGACCTCAGT 
CAGATGCCAC TTTCCCCAGA CCTCAACCAG GCAACTCTCT CTCCAGACAT CAGTCAGACA 
TCCCTTCCTC TTGATCTCAG GCAGACATCA CCTCCTCTAG ATCTCCATCA GACATCCCAC 
ACTTCTGAAT TTAGTCAGTC ATTGCCTCTC CCAGAATTTG GTCAGACTTT CCCTTATGCA 
GATCTTGGTC AGATGCCATC TCCTCCACCA CACTCTCCAC TAAATAACAC TTTTATACCA 
AGAGAATTTA ATCCACTGGT CATAGTAGGC CTCAGTAGAG ATGATGGAGA TTACATTGAA 
ATTATTCCAA GGCAGAAGGA AGAGAGCAGT GAAGAAGACT ATAGTGAATT TGATTTTGTG 
GCTTATGATG ACCCTTACCA AACTGATCTT AGGACAGACA TCAACTCCTC CAGAAATCCT 
GACAACATTG CAGCATGGTA CCTTCGCAGC AACAATGGAA ACAGAAAAAA TTATTACATT 
GCTGCTGAAG AACTATCCTG GGATTATTCA AAATTTGCAC AAAGTGAAGA CACTGATGAT 
GTTTCAAAGG ACACCATATA CAAGAAAGTA GTTTTTCGAA AGTACCTTGA TAGCACTTTT 
AAAAAGCTTG ATCCTCGGGG AGAGTATGAA GAGCATCTTG GCATACTTGG TCCTATCATT 
AGAGCTGAAG TGGATGATGT TATCCAAGTT CGTTTTAAAA ACTTAGCATC CAGACCATAT 
TCTCTTCATG CCCATGGGCT TTCCTATGAA AAATCATCAG AGGGAAAGAC TTATGAAGAT 
GACTCTCCTG AATGGTTTAA GGAAGACAAT GCTGTTCAGC CCAACAGCAC TTATACATAT 
GTATGGCACG CCACTGAGCG ATCAGGGCCA GAAAATCCTG GCTCTGCCTG TCGGGCTTGG 
GCCTACTACT CAGCAGTGAA CCCGGAAAAA GACATCCACT CAGGCTTGAT AGGACCCCTT 
CTGATCTGCC GAAAAGGAAC ACTTCATAAG GAGACCAACA TGCCTGTGGA CATGAGAGAA 
TTTGTCCTAC TTTTTATGGT CTTTGATGAA AAGAAGAGCT GGTACTATGA AAAGAAGCCC 
ATGAGGTCTT GGAGACGCAC ATCCTCAGAA CCATTAATGG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028344492.1
CDS	27..5711
Translation	MFPACPCLWVLVVLGSSWAGWGSLGAEAARLRQFYVAAQSISWNYLPESTNLSSNTFATSFKKIVYREYEAYFQKEKPRSRTSGLLGPTLYAEVGDIMKVHFKNKADKPLSIHPQGIKYSKFSEGASYPDHTFPVERMDDSVAPGQEYTYEWNISEDSGPTHDDPPCLTYIYYSYENLVHDFNSGLIGPLLICKKGTLTEDGIQKMFDKQHVLMFAVFDESKSWNQTSSLMYTVNGYVNGTMPDITVCVHDHISWHLIGMSSGPELFSIHFNGQVLEENHHKMSAITLVSATSTTANMTVSLEGKWIISSLIPKHFQAGMQAYIDIKNCAKKTRNPKKLTRDQRRHIKRWEYFIAAEEVIWDYAPIIPANMDKKYRSLHLDNFSNQIGKHYKKVVYKQYQDASFTKRLENPNVKEDGILGPIIRAQVRDTLKIMFKNMASRSYSIYPHGVTFSPYEDEVNSSFASGSNTMIKAVQPGETYTYKWNILESDEPMENDAQCLTRPYYSNVDITRDIASGLIGLVLICKSRSLDKRGIQRTADIEQQAVFAVFDENKSWYIEDNIYKFCENPEKVKRDDPKFYESNIMSTINGYVPESIPTLGFCFDDTVQWHFCSMGTQNDILTIHFTGHSFIYGKKHEDTLTLFPMRGESVTVTMDNVGTWMLTTMNSNPRSKKLWLRFRDVKCIREYDDDSYEIIYEPSGSTSKTTRKMRDSSEIKDKTNDADYDYQNTLALLLGIRSFRNSSSNQEEDEFNLTALALGNNSEFIPPSTNTTVGSNSSSPGNISRLIANNFAQLLKILPHSEATTAGPPLGHTSLDKNSVLNPPTAEHSSPYSEDPLYSDVTGISLLPFATEEFRNQEHAKRKRFKAGRGRAAKQRFSQMEFLARKTGRHLSKGNSSSSRIGPWENLPSDLLLLQQKDPSKILNGEWHLVSEKGSYEIIQDADEDKAVNKLPNSPQNDSRTWGENIPFKNKHGKQSGHPTFFVIRHKPLQGRQDGGNSRLKKGLFLIRTRRKKKEEKPAHHVPLSPRSFYPLRVEANTPSSDGRHNHSLLLHTSNETSLPTDLNQTFPSMNLSLIASLPDGDQNPPNDTIQTSSPPDLYQTVSPEEHYQTFPIQDSDPTHSTTVPSHRYPPPEPSQIPDYDLRNKAFPTDVSQTFPSLELEVWQTTTSLDLSQPSLYPDLSQTTLSPDPGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPVLSQETLSPDLSQMALSPHPGQETLSPDFSQTTLSPDLSQETLSPDLGQIALSPDLGQETLSPDLSQTTFSPDFSQTISPDLRQETLSPGLNQTALSPDLSQETLSPDLSQTTFSPDLSQETLSPDLSPMALSPDLGQETLSPDLSQTTLSPDLSQALSPDIRQMVLSPDLSQEILSPDLSQTTPSPDLGQETLSADLSQTTLSPDLGQETLSADLSQTTLSPDLGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPDLSQALSPDISQMVLSPDLSQMPLSPDLNQATLSPDISQTSLPLDLRQTSPPLDLHQTSHTSEFSQSLPLPEFGQTFPYADLGQMPSPPPHSPLNNTFIPREFNPLVIVGLSRDDGDYIEIIPRQKEESSEEDYSEFDFVAYDDPYQTDLRTDINSSRNPDNIAAWYLRSNNGNRKNYYIAAEELSWDYSKFAQSEDTDDVSKDTIYKKVVFRKYLDSTFKKLDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKTYEDDSPEWFKEDNAVQPNSTYTYVWHATERSGPENPGSACRAWAYYSAVNPEKDIHSGLIGPLLICRKGTLHKETNMPVDMREFVLLFMVFDEKKSWYYEKKPMRSWRRTSSEPLMG

Target ORF information:

RefSeq Version	XM_028488691.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon coagulation factor V (F5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028488691.1

ORF Insert Sequence:

ATGTTCCCAG CTTGCCCTTG CCTCTGGGTC CTCGTGGTCC TGGGCTCCAG CTGGGCAGGC 
TGGGGGAGCC TAGGGGCTGA AGCAGCGCGG CTAAGGCAGT TCTACGTGGC TGCTCAGAGC 
ATCAGCTGGA ACTATCTCCC CGAGTCCACA AACCTTAGTT CGAATACTTT TGCAACCTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACGATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAGGCAGA CAAACCCTTA AGCATCCATC CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCTTC TTACCCTGAC CACACATTCC CTGTGGAGAG GATGGATGAT 
TCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGAATA TCAGTGAGGA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACATAT ATCTATTACT CCTATGAAAA TCTGGTACAC 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACTGAG 
GATGGGATAC AGAAGATGTT TGACAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCCTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGTCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATTCAT TTCAATGGCC AGGTCCTGGA AGAGAACCAT 
CATAAGATGT CAGCCATCAC TCTCGTCAGT GCTACGTCCA CAACTGCAAA CATGACTGTG 
AGCCTAGAGG GAAAGTGGAT CATATCTTCG CTCATCCCAA AACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GGAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AGTACAGATC TCTGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGCATCCT TCACCAAACG CCTGGAGAAT CCCAATGTCA AAGAAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTTAAAATCA TGTTCAAAAA TATGGCCAGC 
CGCAGCTACA GCATTTACCC TCATGGTGTG ACCTTCTCTC CTTATGAAGA TGAAGTCAAC 
TCTTCCTTCG CCTCAGGCAG TAACACCATG ATCAAAGCAG TTCAACCAGG GGAAACCTAT 
ACTTATAAGT GGAACATCCT AGAGTCTGAT GAACCCATGG AAAATGATGC TCAATGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACA TTGCCTCTGG GCTGATAGGG 
CTAGTTCTAA TTTGTAAGAG CAGATCCTTG GATAAGCGAG GCATACAGAG GACAGCAGAC 
ATCGAGCAAC AGGCTGTGTT TGCTGTGTTT GATGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAGGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGCAC TATCAATGGC TATGTGCCTG AGAGCATACC CACACTAGGA 
TTCTGCTTTG ATGACACCGT CCAGTGGCAC TTCTGCAGTA TGGGAACCCA GAATGACATT 
TTGACCATTC ACTTCACTGG GCACTCATTC ATCTATGGAA AGAAACATGA GGACACCTTG 
ACCCTCTTCC CCATGCGTGG GGAATCTGTG ACCGTCACAA TGGATAACGT TGGAACTTGG 
ATGTTAACTA CCATGAATTC CAATCCAAGA AGCAAAAAAC TGTGGCTGAG ATTCAGGGAT 
GTTAAGTGTA TCCGGGAGTA TGATGATGAC TCATATGAGA TTATATACGA ACCTTCAGGA 
TCTACATCCA AAACTACAAG GAAAATGCGT GATTCTTCAG AAATCAAAGA TAAAACTAAT 
GATGCTGACT ATGATTACCA GAACACACTG GCTTTATTAT TAGGAATCAG GTCATTCAGA 
AATTCATCAT CAAATCAGGA GGAAGATGAG TTCAATCTTA CTGCCCTGGC TCTGGGGAAC 
AACTCTGAAT TCATTCCTCC AAGTACCAAC ACAACTGTTG GTTCAAATTC TTCTTCCCCA 
GGTAACATTA GCAGGCTTAT TGCCAATAAC TTTGCACAAC TTTTGAAAAT TCTTCCTCAC 
TCAGAAGCCA CCACAGCTGG TCCCCCACTG GGACACACCA GCTTAGATAA GAACTCAGTT 
CTCAACCCTC CCACAGCAGA GCATTCCAGC CCTTATTCTG AAGATCCTCT ATACTCAGAT 
GTCACAGGGA TAAGCCTGCT TCCATTTGCT ACAGAAGAAT TCAGAAATCA AGAACATGCT 
AAACGTAAAA GATTCAAGGC AGGAAGAGGC CGAGCAGCAA AGCAAAGGTT CTCCCAGATG 
GAATTCCTAG CACGTAAAAC TGGTAGACAT TTAAGCAAAG GCAACTCTTC TTCTTCCAGA 
ATAGGGCCCT GGGAGAACCT TCCCAGTGAT CTGTTACTCT TGCAACAAAA GGATCCATCT 
AAGATTCTGA ATGGGGAATG GCATTTGGTT TCTGAGAAAG GCAGTTATGA AATAATCCAA 
GATGCTGATG AAGACAAGGC TGTTAATAAG CTGCCAAACA GCCCCCAGAA TGACTCAAGG 
ACTTGGGGAG AAAACATCCC TTTTAAAAAC AAGCATGGAA AGCAGAGTGG CCACCCAACG 
TTTTTTGTAA TTAGACATAA ACCTCTACAA GGAAGACAAG ATGGAGGAAA TAGTAGATTG 
AAGAAAGGCC TTTTTTTAAT TAGGACACGA AGAAAGAAAA AGGAAGAGAA GCCTGCACAC 
CATGTTCCTC TATCTCCAAG GAGTTTTTAC CCTCTAAGAG TAGAGGCCAA CACCCCATCT 
TCAGATGGGA GACATAATCA TTCATTGTTA CTCCATACAT CCAATGAAAC ATCTCTTCCC 
ACAGACCTCA ATCAGACATT CCCCTCTATG AACCTTAGCC TCATAGCCTC ACTTCCTGAT 
GGTGATCAAA ACCCCCCAAA TGACACCATT CAGACAAGCT CCCCTCCAGA TCTTTATCAG 
ACAGTGAGCC CAGAGGAACA CTATCAAACA TTCCCTATTC AAGACTCTGA TCCAACGCAC 
TCCACTACAG TCCCCAGTCA CAGATATCCT CCTCCAGAGC CCAGCCAGAT ACCCGACTAT 
GACCTAAGAA ACAAGGCCTT TCCTACTGAT GTTAGTCAAA CGTTCCCTTC CTTGGAACTT 
GAAGTCTGGC AGACAACCAC CTCTCTAGAC CTCAGTCAGC CATCCCTCTA TCCAGATCTC 
AGCCAGACCA CCCTTTCTCC AGACCCTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT TGGCCAGGAG ACTCTCTCTC CAGACCTCAG CCAGACAACA 
CTTTCCCCAG ACCTTGGCCA GGAGACTCTC TCTCCAGACC TCAGCCAGAC AACACTTTCC 
CCAGTTCTTA GCCAGGAGAC TCTCTCTCCA GACCTCAGCC AGATGGCCCT TTCCCCACAC 
CCTGGCCAGG AGACGCTTTC TCCAGACTTC AGCCAGACAA CACTTTCCCC AGACCTTAGT 
CAGGAGACTC TCTCTCCAGA CCTCGGACAG ATAGCCCTTT CCCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACA TTTTCCCCAG ACTTCAGCCA GACTATCTCT 
CCAGACCTCA GACAGGAGAC TCTCTCTCCA GGCCTCAACC AGACAGCCCT TTCCCCAGAC 
CTTAGCCAGG AGACTCTCTC TCCAGACCTC AGCCAGACAA CATTTTCCCC AGACCTTAGC 
CAGGAGACTC TCTCTCCAGA CCTCAGCCCG ATGGCCCTTT CGCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACG CTTTCCCCAG ACCTCAGCCA GGCTCTCTCT 
CCAGACATCA GACAGATGGT CCTTTCCCCA GACCTCAGCC AGGAGATTCT CTCTCCAGAC 
CTCAGCCAGA CAACACCTTC CCCAGACCTT GGCCAGGAGA CTCTCTCTGC AGACCTCAGC 
CAGACAACAC TTTCCCCAGA CCTTGGCCAG GAGACTCTCT CTGCAGACCT CAGCCAGACA 
ACACTTTCCC CAGACCTCGG CCAGGAGACT CTCTCTCCAG ACCTCAGCCA GACAACACTT 
TCCCCAGACC TTGGCCAGGA GACTCTCTCT CCAGACCTCA GCCAGACAAC ACTTTCCCCA 
GACCTCAGCC AGGCTCTCTC TCCAGACATC AGCCAGATGG TCCTTTCCCC AGACCTCAGT 
CAGATGCCAC TTTCCCCAGA CCTCAACCAG GCAACTCTCT CTCCAGACAT CAGTCAGACA 
TCCCTTCCTC TTGATCTCAG GCAGACATCA CCTCCTCTAG ATCTCCATCA GACATCCCAC 
ACTTCTGAAT TTAGTCAGTC ATTGCCTCTC CCAGAATTTG GTCAGACTTT CCCTTATGCA 
GATCTTGGTC AGATGCCATC TCCTCCACCA CACTCTCCAC TAAATAACAC TTTTATACCA 
AGAGAATTTA ATCCACTGGT CATAGTAGGC CTCAGTAGAG ATGATGGAGA TTACATTGAA 
ATTATTCCAA GGCAGAAGGA AGAGAGCAGT GAAGAAGACT ATAGTGAATT TGATTTTGTG 
GCTTATGATG ACCCTTACCA AACTGATCTT AGGACAGACA TCAACTCCTC CAGAAATCCT 
GACAACATTG CAGCATGGTA CCTTCGCAGC AACAATGGAA ACAGAAAAAA TTATTACATT 
GCTGCTGAAG AACTATCCTG GGATTATTCA AAATTTGCAC AAAGTGAAGA CACTGATGAT 
GTTTCAAAGG ACACCATATA CAAGAAAGTA GTTTTTCGAA AGTACCTTGA TAGCACTTTT 
AAAAAGCTTG ATCCTCGGGG AGAGTATGAA GAGCATCTTG GCATACTTGG TCCTATCATT 
AGAGCTGAAG TGGATGATGT TATCCAAGTT CGTTTTAAAA ACTTAGCATC CAGACCATAT 
TCTCTTCATG CCCATGGGCT TTCCTATGAA AAATCATCAG AGGGAAAGAC TTATGAAGAT 
GACTCTCCTG AATGGTTTAA GGAAGACAAT GCTGTTCAGC CCAACAGCAC TTATACATAT 
GTATGGCACG CCACTGAGCG ATCAGGGCCA GAAAATCCTG GCTCTGCCTG TCGGGCTTGG 
GCCTACTACT CAGCAGTGAA CCCGGAAAAA GACATCCACT CAGGCTTGAT AGGACCCCTT 
CTGATCTGCC GAAAAGGAAC ACTTCATAAG GAGACCAACA TGCCTGTGGA CATGAGAGAA 
TTTGTCCTAC TTTTTATGGT CTTTGATGAA AAGAAGAGCT GGTACTATGA AAAGAAGCCC 
ATGAGGTCTT GGAGACGCAC ATCCTCAGAA CCATTAATGG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F5 ( XM_007102057.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007102119.1 Physeter catodon coagulation factor V (proaccelerin, labile factor) (F5), mRNA.

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CloneID	OPh112271
Clone ID Related Accession (Same CDS sequence)	XM_007102057.1
Accession Version	XM_007102057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	coagulation factor V
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006712928.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAG CTTGCCCTTG CCTCTGGGTC CTCGTGGTCC TGGGCTCCAG CTGGGCAGGC 
TGGGGGAGCC TAGGGGCTGA AGCAGCGCGG CTAAGGCAGT TCTACGTGGC TGCTCAGAGC 
ATCAGCTGGA ACTATCTCCC CGAGTCCACA AACCTTAGTT CGAATACTTT TGCAACCTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACGATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAGGCAGA CAAACCCTTA AGCATCCATC CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCTTC TTACCCTGAC CACACATTCC CTGTGGAGAG GATGGATGAT 
TCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGAATA TCAGTGAGGA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACATAT ATCTATTACT CCTATGAAAA TCTGGTACAC 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACTGAG 
GATGGGATAC AGAAGATGTT TGACAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCCTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGTCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATTCAT TTCAATGGCC AGGTCCTGGA AGAGAACCAT 
CATAAGATGT CAGCCATCAC TCTCGTCAGT GCTACGTCCA CAACTGCAAA CATGACTGTG 
AGCCTAGAGG GAAAGTGGAT CATATCTTCG CTCATCCCAA AACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GGAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AGTACAGATC TCTGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGCATCCT TCACCAAACG CCTGGAGAAT CCCAATGTCA AAGAAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTTAAAATCA TGTTCAAAAA TATGGCCAGC 
CGCAGCTACA GCATTTACCC TCATGGTGTG ACCTTCTCTC CTTATGAAGA TGAAGTCAAC 
TCTTCCTTCG CCTCAGGCAG TAACACCATG ATCAAAGCAG TTCAACCAGG GGAAACCTAT 
ACTTATAAGT GGAACATCCT AGATTCTGAT GAACCCATGG AAAATGATGC TCAATGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACA TTGCCTCTGG GCTGATAGGG 
CTAGTTCTAA TTTGTAAGAG CAGATCCTTG GATAAGCGAG GCATACAGAG GACAGCAGAC 
ATCGAGCAAC AGGCTGTGTT TGCTGTGTTT GATGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAGGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGCAC TATCAATGGC TATGTGCCTG AGAGCATACC CACACTAGGA 
TTCTGCTTTG ATGACACCGT CCAGTGGCAC TTCTGCAGTA TGGGAACCCA GAATGACATT 
TTGACCATTC ACTTCACTGG GCACTCATTC ATCTATGGAA AGAAACATGA GGACACCTTG 
ACCCTCTTCC CCATGCGTGG GGAATCTGTG ACCGTCACAA TGGATAACGT TGGAACTTGG 
ATGTTAACTA CCATGAATTC CAATCCAAGA AGCAAAAAAC TGTGGCTGAG ATTCAGGGAT 
GTTAAGTGTA TCCGGGAGTA TGATGATGAC TCATATGAGA TTATATACGA ACCTTCAGGA 
TCTACATCCA AAACTACAAG GAAAATGCGT GATTCTTCAG AAATCAAAGA TAAAACTAAT 
GATGCTGACT ATGATTACCA GAACACACTG GCTTTATTAT TAGGAATCAG GTCATTCAGA 
AATTCATCAT CAAATCAGGA GGAAGATGAG TTCAATCTTA CTGCCCTGGC TCTGGGGAAC 
AACTCTGAAT TCATTCCTCC AAGTACCAAC AGAACTGTTG GTTCAAATTC TTCTTCCCCA 
GGTAACATTA GCAGGCTTAT TGCCAATAAC TTTGCACAAC TTTTGAAAAT TCTTCCTCAC 
TCAGAAGCCA CCACAGCTGG TCCCCCACTG GGACACACCA GCTTAGATAA GAACTCAGTT 
CTCAACCCTC CCACAGCAGA GCATTCCAGC CCTTATTCTG AAGATCCTCT ATACTCAGAT 
GTCACAGGGA TAAGCCTGCT TCCATTTGCT ACAGAAGAAT TCAGAAATCA AGAACATGCT 
AAACGTAAAA GATTCAAGGC AGGAAGAGGC CGAGCAGCAA AGCAAAGGTT CTCCCAGATG 
GAATTCCTAG CACGTAAAAC TGGTAGACAT TTAAGCAAAG GCAACTCTTC TTCTTCCAGA 
ATAGGGCCCT GGGAGAACCT TCCCAGTGAT CTGTTACTCT TGCAACAAAA GGATCCATCT 
AAGATTCTGA ATGGGGAATG GCATTTGGTT TCTGAGAAAG GCAGTTATGA AATAATCCAA 
GATGCTGATG AAGACAAGGC TGTTAATAAG CTGCCAAACA GCCCCCAGAA TGACTCAAGG 
ACTTGGGGAG AAAACATCCC TTTTAAAAAC AAGCATGGAA AGCAGAGTGG CCACCCAACG 
TTTTTTGTAA TTAGACATAA ACCTCTACAA GGAAGACAAG ATGGAGGAAA TAGTAGATTG 
AAGAAAGGCC TTTTTTTAAT TAGGACACGA AGAAAGAAAA AGGAAGAGAA GCCTGCACAC 
CATGTTCCTC TATCTCCAAG GAGTTTTTAC CCTCTAAGAG TAGAGGCCAA CACCCCATCT 
TCAGATGGGA GACATAATCA TTCATTGTTA CTCCATACAT CCAATGAAAC ATCTCTTCCC 
ACAGACCTCA ATCAGACATT CCCCTCTATG AACCTTAGCC TCATAGCCTC ACTTCCTGAT 
GGTGATCAAA ACCCCCCAAA TGACACCATT CAGACAAGCT CCCCTCCAGA TCTTTATCAG 
ACAGTGAGCC CAGAGGAACA CTATCAAACA TTCCCTATTC AAGACTCTGA TCCAACGCAC 
TCCACTACAG TCCCCAGTCA CAGATATCCT CCTCCAGAGC CCAGCCAGAT ACCCGACTAT 
GACCTAAGAA ACAAGGCCTT TCCTACTGAT GTTAGTCAAA CGTTCCCTTC CTTGGAACTT 
GAAGTCTGGC AGACAACCAC CTCTCTAGAC CTCAGTCATC CATCCCTCTA TCCAGATCTC 
AGCCAGACCA CCCTTTCTCC AGACCCTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT TGGCCAGGAG ACTCTCTCTC CAGACCTCAG CCAGACAACA 
CTTTCCCCNN NNNNNNNNNN NNCAACACTT TCCCCAGACC TCGGCCAGGA GACTCTCTCT 
CCAGACCTCA GCCAGACAAC ACTTTCCCCA GACCTTGGCC AGGAGACTCT CTCTCCAGAC 
CTCAGCCAGA CAACACTTTC CCCAGACCTC AGCCAGGCTC TCTCTCCAGA CATCAGCCAG 
ATGGTCCTTT CCCCAGACCT CAGTCAGATG CCACTTTCCC CAGACCTCAA CCAGGCAACT 
CTCTCTCCAG ACATCAGTCA GACATCCCTT CCTCTTGATC TCAGGCAGAC ATCACCTCCT 
CTAGATCTCC ATCAGACATC CCACACTTCT GAATTTAGTC AGTCATTGCC TCTCCCAGAA 
TTTGGTCAGA CTTTCCCTTA TGCAGATCTT GGTCAGATGC CATCTCCTCC ACCACACTCT 
CCACTAAATA ACACTTTTAT ACCAAGAGAA TTTAATCCAC TGGTCATAGT AGGCCTCAGT 
AGAGATGATG GAGATTACAT TGAAATTATT CCAAGGCAGA AGGAAGAGAG CAGTGAAGAA 
GACTATAGTG AATTTGATTT TGTGGCTTAT GATGACCCTT ACCAAACTGA TCTTAGGACA 
GACATCAACT CCTCCAGAAA TCCTGACAAC ATTGCAGCAT GGTACCTTCG CAGCAACAAT 
GGAAACAGAA AAAATTATTA CATTGCTGCT GAAGAACTAT CCTGGGATTA TTCAAAATTT 
GCACAAAGTG AAGACACTGA TGATGTTTCA AAGGACACCA TATACAAGAA AGTAGTTTTT 
CGAAAGTACC TTGATAGCAC TTTTAAAAAG CTTGATCCTC GGGGAGAGTA TGAAGAGCAT 
CTTGGCATAC TTGGTCCTAT CATTAGAGCT GAAGTGGATG ATGTTATCCA AGTTCGTTTT 
AAAAACTTAG CATCCAGACC ATATTCTCTT CATGCCCATG GGCTTTCCTA TGAAAAATCA 
TCAGAGGGAA AGACTTATGA AGATGACTCT CCTGAATGGT TTAAGGAAGA CAATGCTGTT 
CAGCCCAACA GCACTTATAC ATATGTATGG CACGCCACTG AGCGATCAGG GCCAGAAAAT 
CCTGGCTCTG CCTGTCGGGC TTGGGCCTAC TACTCAGCAG TGAACCCGGA AAAAGACATC 
CACTCAGGCT TGATAGGACC CCTTCTGATC TGCCGAAAAG GAACACTTCA TAAGGAGACC 
AACATGCCTG TGGACATGAG AGAATTTGTC CTACTTTTTA TGGTCTTTGA TGAAAAGAAG 
AGCTGGTACT ATGAAAAGAA GCCCATGAGG TCTTGGAGAC GCACATCCTC AGAAGTAAAA 
AACTCCCATG AGTTTCATGC CATTAATGGG ATGATCTACA ACTTGCCTGG CCTGAGAATG 
TACGAGCAAG AGTGGGTGAG GTTGCACCTG CTGAATGTAG GCGGCTCCCA GGACATTCAT 
GTGGTTCACT TTCATGGCCA GACCTTGCTG GAAAATGGCA CTCAACAGTA CCAGTTAGGG 
GTCTGGCCTC TTCTGCCTGG TTCATTTAAA ACTCTTGAAA TGAAGGCTTC AAAACCTGGC 
TGGTGGCTCC TAGACACAGA AGTTGGAGAA AACCAGAGAG CAGGGATGCA AACGCTATTT 
CTCATCACAG ACAGAGACTG TAAAATGCCA ATGGGACTAA GCACTGGTCT CATAGCTGAT 
TCACAGATCA AGGCTTCTGA GTTTTGGGGT TATTGGGAAC CCAAATTAGC AAGACTAAAC 
AATGGTGGAT CATATAATGC TTGGATTGCA GAAAAACTTT CAGTAGAATT TAACCCTAAA 
CCTTGGATCC AGGTAGACAT GCAAAGGGAA GTCCTGTTCA CGGGGATCCA GACCCAAGGT 
GCCAAACACT ACCTGAAGTC TTACTACACC ACCGAGTTCT TTGTGGCTTA CAGCTCTGAC 
CAGACAAACT GGCGGATCTT CAAAGGGAAC AGCACTAGGA ACGTGATGTA TTTTAATGGC 
AATTCAGATG CCTCTACAAT AAAAGAGAAT CAGTTTGACC CACCTGTTGT GGCTAGATAT 
ATTAGAATCT CTCCAACTAA ATCCTATAAC AAACCTGCCC TTCGATTGGA ACTGCAAGGT 
TGCGAGGTTA ACGGATGCTC CACACCTCTG GGTATGGAAA GTGGAAAGAT AGAAGACAAG 
CAAATCACAG CTTCCTCATT TAAAAAATCT TGGTGGGGAG ATTACTGGGA ACCCTTCTGT 
GCCCGTCTTA ATGCCCAGGG CCGTGTGAAT GCCTGGCAAG CCAAGGCAAA CAACAACAAT 
CAGTGGTTAC AAATTGATCT GCTCAAAATC AAGAAGATAA CAGCAATTAT AACACAGGGT 
TGCAAGTCTC TGTCCTCTGA AATGTATGTG AAGAGCTACA CCATCCAGTA CAGTGACCAG 
GGAGTTGAAT GGAAACCTTA CAGAGAGAAA TCCTCGTTGG TGGACAAGGT ACAGGGGTAT 
CTTGGCAAAA AGAAAACAAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007102119.1
CDS	26..6169
Translation	MFPACPCLWVLVVLGSSWAGWGSLGAEAARLRQFYVAAQSISWNYLPESTNLSSNTFATSFKKIVYREYEAYFQKEKPRSRTSGLLGPTLYAEVGDIMKVHFKNKADKPLSIHPQGIKYSKFSEGASYPDHTFPVERMDDSVAPGQEYTYEWNISEDSGPTHDDPPCLTYIYYSYENLVHDFNSGLIGPLLICKKGTLTEDGIQKMFDKQHVLMFAVFDESKSWNQTSSLMYTVNGYVNGTMPDITVCVHDHISWHLIGMSSGPELFSIHFNGQVLEENHHKMSAITLVSATSTTANMTVSLEGKWIISSLIPKHFQAGMQAYIDIKNCAKKTRNPKKLTRDQRRHIKRWEYFIAAEEVIWDYAPIIPANMDKKYRSLHLDNFSNQIGKHYKKVVYKQYQDASFTKRLENPNVKEDGILGPIIRAQVRDTLKIMFKNMASRSYSIYPHGVTFSPYEDEVNSSFASGSNTMIKAVQPGETYTYKWNILDSDEPMENDAQCLTRPYYSNVDITRDIASGLIGLVLICKSRSLDKRGIQRTADIEQQAVFAVFDENKSWYIEDNIYKFCENPEKVKRDDPKFYESNIMSTINGYVPESIPTLGFCFDDTVQWHFCSMGTQNDILTIHFTGHSFIYGKKHEDTLTLFPMRGESVTVTMDNVGTWMLTTMNSNPRSKKLWLRFRDVKCIREYDDDSYEIIYEPSGSTSKTTRKMRDSSEIKDKTNDADYDYQNTLALLLGIRSFRNSSSNQEEDEFNLTALALGNNSEFIPPSTNRTVGSNSSSPGNISRLIANNFAQLLKILPHSEATTAGPPLGHTSLDKNSVLNPPTAEHSSPYSEDPLYSDVTGISLLPFATEEFRNQEHAKRKRFKAGRGRAAKQRFSQMEFLARKTGRHLSKGNSSSSRIGPWENLPSDLLLLQQKDPSKILNGEWHLVSEKGSYEIIQDADEDKAVNKLPNSPQNDSRTWGENIPFKNKHGKQSGHPTFFVIRHKPLQGRQDGGNSRLKKGLFLIRTRRKKKEEKPAHHVPLSPRSFYPLRVEANTPSSDGRHNHSLLLHTSNETSLPTDLNQTFPSMNLSLIASLPDGDQNPPNDTIQTSSPPDLYQTVSPEEHYQTFPIQDSDPTHSTTVPSHRYPPPEPSQIPDYDLRNKAFPTDVSQTFPSLELEVWQTTTSLDLSHPSLYPDLSQTTLSPDPGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSnullnullnullnullnullnullTLSPDLGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPDLSQALSPDISQMVLSPDLSQMPLSPDLNQATLSPDISQTSLPLDLRQTSPPLDLHQTSHTSEFSQSLPLPEFGQTFPYADLGQMPSPPPHSPLNNTFIPREFNPLVIVGLSRDDGDYIEIIPRQKEESSEEDYSEFDFVAYDDPYQTDLRTDINSSRNPDNIAAWYLRSNNGNRKNYYIAAEELSWDYSKFAQSEDTDDVSKDTIYKKVVFRKYLDSTFKKLDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKTYEDDSPEWFKEDNAVQPNSTYTYVWHATERSGPENPGSACRAWAYYSAVNPEKDIHSGLIGPLLICRKGTLHKETNMPVDMREFVLLFMVFDEKKSWYYEKKPMRSWRRTSSEVKNSHEFHAINGMIYNLPGLRMYEQEWVRLHLLNVGGSQDIHVVHFHGQTLLENGTQQYQLGVWPLLPGSFKTLEMKASKPGWWLLDTEVGENQRAGMQTLFLITDRDCKMPMGLSTGLIADSQIKASEFWGYWEPKLARLNNGGSYNAWIAEKLSVEFNPKPWIQVDMQREVLFTGIQTQGAKHYLKSYYTTEFFVAYSSDQTNWRIFKGNSTRNVMYFNGNSDASTIKENQFDPPVVARYIRISPTKSYNKPALRLELQGCEVNGCSTPLGMESGKIEDKQITASSFKKSWWGDYWEPFCARLNAQGRVNAWQAKANNNNQWLQIDLLKIKKITAIITQGCKSLSSEMYVKSYTIQYSDQGVEWKPYREKSSLVDKVQGYLGKKKTI

Target ORF information:

RefSeq Version	XM_007102057.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon coagulation factor V (proaccelerin, labile factor) (F5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007102057.1

ORF Insert Sequence:

ATGTTCCCAG CTTGCCCTTG CCTCTGGGTC CTCGTGGTCC TGGGCTCCAG CTGGGCAGGC 
TGGGGGAGCC TAGGGGCTGA AGCAGCGCGG CTAAGGCAGT TCTACGTGGC TGCTCAGAGC 
ATCAGCTGGA ACTATCTCCC CGAGTCCACA AACCTTAGTT CGAATACTTT TGCAACCTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACGATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAGGCAGA CAAACCCTTA AGCATCCATC CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCTTC TTACCCTGAC CACACATTCC CTGTGGAGAG GATGGATGAT 
TCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGAATA TCAGTGAGGA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACATAT ATCTATTACT CCTATGAAAA TCTGGTACAC 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACTGAG 
GATGGGATAC AGAAGATGTT TGACAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCCTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGTCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATTCAT TTCAATGGCC AGGTCCTGGA AGAGAACCAT 
CATAAGATGT CAGCCATCAC TCTCGTCAGT GCTACGTCCA CAACTGCAAA CATGACTGTG 
AGCCTAGAGG GAAAGTGGAT CATATCTTCG CTCATCCCAA AACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GGAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AGTACAGATC TCTGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGCATCCT TCACCAAACG CCTGGAGAAT CCCAATGTCA AAGAAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTTAAAATCA TGTTCAAAAA TATGGCCAGC 
CGCAGCTACA GCATTTACCC TCATGGTGTG ACCTTCTCTC CTTATGAAGA TGAAGTCAAC 
TCTTCCTTCG CCTCAGGCAG TAACACCATG ATCAAAGCAG TTCAACCAGG GGAAACCTAT 
ACTTATAAGT GGAACATCCT AGATTCTGAT GAACCCATGG AAAATGATGC TCAATGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACA TTGCCTCTGG GCTGATAGGG 
CTAGTTCTAA TTTGTAAGAG CAGATCCTTG GATAAGCGAG GCATACAGAG GACAGCAGAC 
ATCGAGCAAC AGGCTGTGTT TGCTGTGTTT GATGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAGGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGCAC TATCAATGGC TATGTGCCTG AGAGCATACC CACACTAGGA 
TTCTGCTTTG ATGACACCGT CCAGTGGCAC TTCTGCAGTA TGGGAACCCA GAATGACATT 
TTGACCATTC ACTTCACTGG GCACTCATTC ATCTATGGAA AGAAACATGA GGACACCTTG 
ACCCTCTTCC CCATGCGTGG GGAATCTGTG ACCGTCACAA TGGATAACGT TGGAACTTGG 
ATGTTAACTA CCATGAATTC CAATCCAAGA AGCAAAAAAC TGTGGCTGAG ATTCAGGGAT 
GTTAAGTGTA TCCGGGAGTA TGATGATGAC TCATATGAGA TTATATACGA ACCTTCAGGA 
TCTACATCCA AAACTACAAG GAAAATGCGT GATTCTTCAG AAATCAAAGA TAAAACTAAT 
GATGCTGACT ATGATTACCA GAACACACTG GCTTTATTAT TAGGAATCAG GTCATTCAGA 
AATTCATCAT CAAATCAGGA GGAAGATGAG TTCAATCTTA CTGCCCTGGC TCTGGGGAAC 
AACTCTGAAT TCATTCCTCC AAGTACCAAC AGAACTGTTG GTTCAAATTC TTCTTCCCCA 
GGTAACATTA GCAGGCTTAT TGCCAATAAC TTTGCACAAC TTTTGAAAAT TCTTCCTCAC 
TCAGAAGCCA CCACAGCTGG TCCCCCACTG GGACACACCA GCTTAGATAA GAACTCAGTT 
CTCAACCCTC CCACAGCAGA GCATTCCAGC CCTTATTCTG AAGATCCTCT ATACTCAGAT 
GTCACAGGGA TAAGCCTGCT TCCATTTGCT ACAGAAGAAT TCAGAAATCA AGAACATGCT 
AAACGTAAAA GATTCAAGGC AGGAAGAGGC CGAGCAGCAA AGCAAAGGTT CTCCCAGATG 
GAATTCCTAG CACGTAAAAC TGGTAGACAT TTAAGCAAAG GCAACTCTTC TTCTTCCAGA 
ATAGGGCCCT GGGAGAACCT TCCCAGTGAT CTGTTACTCT TGCAACAAAA GGATCCATCT 
AAGATTCTGA ATGGGGAATG GCATTTGGTT TCTGAGAAAG GCAGTTATGA AATAATCCAA 
GATGCTGATG AAGACAAGGC TGTTAATAAG CTGCCAAACA GCCCCCAGAA TGACTCAAGG 
ACTTGGGGAG AAAACATCCC TTTTAAAAAC AAGCATGGAA AGCAGAGTGG CCACCCAACG 
TTTTTTGTAA TTAGACATAA ACCTCTACAA GGAAGACAAG ATGGAGGAAA TAGTAGATTG 
AAGAAAGGCC TTTTTTTAAT TAGGACACGA AGAAAGAAAA AGGAAGAGAA GCCTGCACAC 
CATGTTCCTC TATCTCCAAG GAGTTTTTAC CCTCTAAGAG TAGAGGCCAA CACCCCATCT 
TCAGATGGGA GACATAATCA TTCATTGTTA CTCCATACAT CCAATGAAAC ATCTCTTCCC 
ACAGACCTCA ATCAGACATT CCCCTCTATG AACCTTAGCC TCATAGCCTC ACTTCCTGAT 
GGTGATCAAA ACCCCCCAAA TGACACCATT CAGACAAGCT CCCCTCCAGA TCTTTATCAG 
ACAGTGAGCC CAGAGGAACA CTATCAAACA TTCCCTATTC AAGACTCTGA TCCAACGCAC 
TCCACTACAG TCCCCAGTCA CAGATATCCT CCTCCAGAGC CCAGCCAGAT ACCCGACTAT 
GACCTAAGAA ACAAGGCCTT TCCTACTGAT GTTAGTCAAA CGTTCCCTTC CTTGGAACTT 
GAAGTCTGGC AGACAACCAC CTCTCTAGAC CTCAGTCATC CATCCCTCTA TCCAGATCTC 
AGCCAGACCA CCCTTTCTCC AGACCCTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT TGGCCAGGAG ACTCTCTCTC CAGACCTCAG CCAGACAACA 
CTTTCCCCNN NNNNNNNNNN NNCAACACTT TCCCCAGACC TCGGCCAGGA GACTCTCTCT 
CCAGACCTCA GCCAGACAAC ACTTTCCCCA GACCTTGGCC AGGAGACTCT CTCTCCAGAC 
CTCAGCCAGA CAACACTTTC CCCAGACCTC AGCCAGGCTC TCTCTCCAGA CATCAGCCAG 
ATGGTCCTTT CCCCAGACCT CAGTCAGATG CCACTTTCCC CAGACCTCAA CCAGGCAACT 
CTCTCTCCAG ACATCAGTCA GACATCCCTT CCTCTTGATC TCAGGCAGAC ATCACCTCCT 
CTAGATCTCC ATCAGACATC CCACACTTCT GAATTTAGTC AGTCATTGCC TCTCCCAGAA 
TTTGGTCAGA CTTTCCCTTA TGCAGATCTT GGTCAGATGC CATCTCCTCC ACCACACTCT 
CCACTAAATA ACACTTTTAT ACCAAGAGAA TTTAATCCAC TGGTCATAGT AGGCCTCAGT 
AGAGATGATG GAGATTACAT TGAAATTATT CCAAGGCAGA AGGAAGAGAG CAGTGAAGAA 
GACTATAGTG AATTTGATTT TGTGGCTTAT GATGACCCTT ACCAAACTGA TCTTAGGACA 
GACATCAACT CCTCCAGAAA TCCTGACAAC ATTGCAGCAT GGTACCTTCG CAGCAACAAT 
GGAAACAGAA AAAATTATTA CATTGCTGCT GAAGAACTAT CCTGGGATTA TTCAAAATTT 
GCACAAAGTG AAGACACTGA TGATGTTTCA AAGGACACCA TATACAAGAA AGTAGTTTTT 
CGAAAGTACC TTGATAGCAC TTTTAAAAAG CTTGATCCTC GGGGAGAGTA TGAAGAGCAT 
CTTGGCATAC TTGGTCCTAT CATTAGAGCT GAAGTGGATG ATGTTATCCA AGTTCGTTTT 
AAAAACTTAG CATCCAGACC ATATTCTCTT CATGCCCATG GGCTTTCCTA TGAAAAATCA 
TCAGAGGGAA AGACTTATGA AGATGACTCT CCTGAATGGT TTAAGGAAGA CAATGCTGTT 
CAGCCCAACA GCACTTATAC ATATGTATGG CACGCCACTG AGCGATCAGG GCCAGAAAAT 
CCTGGCTCTG CCTGTCGGGC TTGGGCCTAC TACTCAGCAG TGAACCCGGA AAAAGACATC 
CACTCAGGCT TGATAGGACC CCTTCTGATC TGCCGAAAAG GAACACTTCA TAAGGAGACC 
AACATGCCTG TGGACATGAG AGAATTTGTC CTACTTTTTA TGGTCTTTGA TGAAAAGAAG 
AGCTGGTACT ATGAAAAGAA GCCCATGAGG TCTTGGAGAC GCACATCCTC AGAAGTAAAA 
AACTCCCATG AGTTTCATGC CATTAATGGG ATGATCTACA ACTTGCCTGG CCTGAGAATG 
TACGAGCAAG AGTGGGTGAG GTTGCACCTG CTGAATGTAG GCGGCTCCCA GGACATTCAT 
GTGGTTCACT TTCATGGCCA GACCTTGCTG GAAAATGGCA CTCAACAGTA CCAGTTAGGG 
GTCTGGCCTC TTCTGCCTGG TTCATTTAAA ACTCTTGAAA TGAAGGCTTC AAAACCTGGC 
TGGTGGCTCC TAGACACAGA AGTTGGAGAA AACCAGAGAG CAGGGATGCA AACGCTATTT 
CTCATCACAG ACAGAGACTG TAAAATGCCA ATGGGACTAA GCACTGGTCT CATAGCTGAT 
TCACAGATCA AGGCTTCTGA GTTTTGGGGT TATTGGGAAC CCAAATTAGC AAGACTAAAC 
AATGGTGGAT CATATAATGC TTGGATTGCA GAAAAACTTT CAGTAGAATT TAACCCTAAA 
CCTTGGATCC AGGTAGACAT GCAAAGGGAA GTCCTGTTCA CGGGGATCCA GACCCAAGGT 
GCCAAACACT ACCTGAAGTC TTACTACACC ACCGAGTTCT TTGTGGCTTA CAGCTCTGAC 
CAGACAAACT GGCGGATCTT CAAAGGGAAC AGCACTAGGA ACGTGATGTA TTTTAATGGC 
AATTCAGATG CCTCTACAAT AAAAGAGAAT CAGTTTGACC CACCTGTTGT GGCTAGATAT 
ATTAGAATCT CTCCAACTAA ATCCTATAAC AAACCTGCCC TTCGATTGGA ACTGCAAGGT 
TGCGAGGTTA ACGGATGCTC CACACCTCTG GGTATGGAAA GTGGAAAGAT AGAAGACAAG 
CAAATCACAG CTTCCTCATT TAAAAAATCT TGGTGGGGAG ATTACTGGGA ACCCTTCTGT 
GCCCGTCTTA ATGCCCAGGG CCGTGTGAAT GCCTGGCAAG CCAAGGCAAA CAACAACAAT 
CAGTGGTTAC AAATTGATCT GCTCAAAATC AAGAAGATAA CAGCAATTAT AACACAGGGT 
TGCAAGTCTC TGTCCTCTGA AATGTATGTG AAGAGCTACA CCATCCAGTA CAGTGACCAG 
GGAGTTGAAT GGAAACCTTA CAGAGAGAAA TCCTCGTTGG TGGACAAGGT ACAGGGGTAT 
CTTGGCAAAA AGAAAACAAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F5 ( XM_024116689.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023972457.1 Physeter catodon coagulation factor V (F5), transcript variant X1, mRNA.

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CloneID	OPh305667
Clone ID Related Accession (Same CDS sequence)	XM_024116689.1
Accession Version	XM_024116689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	coagulation factor V isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873626.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAG CTTGCCCTTG CCTCTGGGTC CTCGTGGTCC TGGGCTCCAG CTGGGCAGGC 
TGGGGGAGCC TAGGGGCTGA AGCAGCGCGG CTAAGGCAGT TCTACGTGGC TGCTCAGAGC 
ATCAGCTGGA ACTATCTCCC CGAGTCCACA AACCTTAGTT CGAATACTTT TGCAACCTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACGATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAGGCAGA CAAACCCTTA AGCATCCATC CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCTTC TTACCCTGAC CACACATTCC CTGTGGAGAG GATGGATGAT 
TCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGAATA TCAGTGAGGA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACATAT ATCTATTACT CCTATGAAAA TCTGGTACAC 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACTGAG 
GATGGGATAC AGAAGATGTT TGACAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCCTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGTCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATTCAT TTCAATGGCC AGGTCCTGGA AGAGAACCAT 
CATAAGATGT CAGCCATCAC TCTCGTCAGT GCTACGTCCA CAACTGCAAA CATGACTGTG 
AGCCTAGAGG GAAAGTGGAT CATATCTTCG CTCATCCCAA AACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GGAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AGTACAGATC TCTGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGCATCCT TCACCAAACG CCTGGAGAAT CCCAATGTCA AAGAAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTTAAAATCA TGTTCAAAAA TATGGCCAGC 
CGCAGCTACA GCATTTACCC TCATGGTGTG ACCTTCTCTC CTTATGAAGA TGAAGTCAAC 
TCTTCCTTCG CCTCAGGCAG TAACACCATG ATCAAAGCAG TTCAACCAGG GGAAACCTAT 
ACTTATAAGT GGAACATCCT AGAGTCTGAT GAACCCATGG AAAATGATGC TCAATGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACA TTGCCTCTGG GCTGATAGGG 
CTAGTTCTAA TTTGTAAGAG CAGATCCTTG GATAAGCGAG GCATACAGAG GACAGCAGAC 
ATCGAGCAAC AGGCTGTGTT TGCTGTGTTT GATGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAGGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGCAC TATCAATGGC TATGTGCCTG AGAGCATACC CACACTAGGA 
TTCTGCTTTG ATGACACCGT CCAGTGGCAC TTCTGCAGTA TGGGAACCCA GAATGACATT 
TTGACCATTC ACTTCACTGG GCACTCATTC ATCTATGGAA AGAAACATGA GGACACCTTG 
ACCCTCTTCC CCATGCGTGG GGAATCTGTG ACCGTCACAA TGGATAACGT TGGAACTTGG 
ATGTTAACTA CCATGAATTC CAATCCAAGA AGCAAAAAAC TGTGGCTGAG ATTCAGGGAT 
GTTAAGTGTA TCCGGGAGTA TGATGATGAC TCATATGAGA TTATATACGA ACCTTCAGGA 
TCTACATCCA AAACTACAAG GAAAATGCGT GATTCTTCAG AAATCAAAGA TAAAACTAAT 
GATGCTGACT ATGATTACCA GAACACACTG GCTTTATTAT TAGGAATCAG GTCATTCAGA 
AATTCATCAT CAAATCAGGA GGAAGATGAG TTCAATCTTA CTGCCCTGGC TCTGGGGAAC 
AACTCTGAAT TCATTCCTCC AAGTACCAAC ACAACTGTTG GTTCAAATTC TTCTTCCCCA 
GGTAACATTA GCAGGCTTAT TGCCAATAAC TTTGCACAAC TTTTGAAAAT TCTTCCTCAC 
TCAGAAGCCA CCACAGCTGG TCCCCCACTG GGACACACCA GCTTAGATAA GAACTCAGTT 
CTCAACCCTC CCACAGCAGA GCATTCCAGC CCTTATTCTG AAGATCCTCT ATACTCAGAT 
GTCACAGGGA TAAGCCTGCT TCCATTTGCT ACAGAAGAAT TCAGAAATCA AGAACATGCT 
AAACGTAAAA GATTCAAGGC AGGAAGAGGC CGAGCAGCAA AGCAAAGGTT CTCCCAGATG 
GAATTCCTAG CACGTAAAAC TGGTAGACAT TTAAGCAAAG GCAACTCTTC TTCTTCCAGA 
ATAGGGCCCT GGGAGAACCT TCCCAGTGAT CTGTTACTCT TGCAACAAAA GGATCCATCT 
AAGATTCTGA ATGGGGAATG GCATTTGGTT TCTGAGAAAG GCAGTTATGA AATAATCCAA 
GATGCTGATG AAGACAAGGC TGTTAATAAG CTGCCAAACA GCCCCCAGAA TGACTCAAGG 
ACTTGGGGAG AAAACATCCC TTTTAAAAAC AAGCATGGAA AGCAGAGTGG CCACCCAACG 
TTTTTTGTAA TTAGACATAA ACCTCTACAA GGAAGACAAG ATGGAGGAAA TAGTAGATTG 
AAGAAAGGCC TTTTTTTAAT TAGGACACGA AGAAAGAAAA AGGAAGAGAA GCCTGCACAC 
CATGTTCCTC TATCTCCAAG GAGTTTTTAC CCTCTAAGAG TAGAGGCCAA CACCCCATCT 
TCAGATGGGA GACATAATCA TTCATTGTTA CTCCATACAT CCAATGAAAC ATCTCTTCCC 
ACAGACCTCA ATCAGACATT CCCCTCTATG AACCTTAGCC TCATAGCCTC ACTTCCTGAT 
GGTGATCAAA ACCCCCCAAA TGACACCATT CAGACAAGCT CCCCTCCAGA TCTTTATCAG 
ACAGTGAGCC CAGAGGAACA CTATCAAACA TTCCCTATTC AAGACTCTGA TCCAACGCAC 
TCCACTACAG TCCCCAGTCA CAGATATCCT CCTCCAGAGC CCAGCCAGAT ACCCGACTAT 
GACCTAAGAA ACAAGGCCTT TCCTACTGAT GTTAGTCAAA CGTTCCCTTC CTTGGAACTT 
GAAGTCTGGC AGACAACCAC CTCTCTAGAC CTCAGTCAGC CATCCCTCTA TCCAGATCTC 
AGCCAGACCA CCCTTTCTCC AGACCCTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT TGGCCAGGAG ACTCTCTCTC CAGACCTCAG CCAGACAACA 
CTTTCCCCAG ACCTTGGCCA GGAGACTCTC TCTCCAGACC TCAGCCAGAC AACACTTTCC 
CCAGTTCTTA GCCAGGAGAC TCTCTCTCCA GACCTCAGCC AGATGGCCCT TTCCCCACAC 
CCTGGCCAGG AGACGCTTTC TCCAGACTTC AGCCAGACAA CACTTTCCCC AGACCTTAGT 
CAGGAGACTC TCTCTCCAGA CCTCGGACAG ATAGCCCTTT CCCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACA TTTTCCCCAG ACTTCAGCCA GACTATCTCT 
CCAGACCTCA GACAGGAGAC TCTCTCTCCA GGCCTCAACC AGACAGCCCT TTCCCCAGAC 
CTTAGCCAGG AGACTCTCTC TCCAGACCTC AGCCAGACAA CATTTTCCCC AGACCTTAGC 
CAGGAGACTC TCTCTCCAGA CCTCAGCCCG ATGGCCCTTT CGCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACG CTTTCCCCAG ACCTCAGCCA GACAACACTT 
TCCCCAGACC TTGGCCAGGA GACTCTCTCT GCAGACCTCA GCCAGACAAC ACTTTCCCCA 
GACCTCGGCC AGGAGACTCT CTCTCCAGAC CTCAGCCAGA CAACACTTTC CCCAGACCTT 
GGCCAGGAGA CTCTCTCTCC AGACCTCAGC CAGACAACAC TTTCCCCAGA CCTCAGCCAG 
GCTCTCTCTC CAGACATCAG CCAGATGGTC CTTTCCCCAG ACCTCAGTCA GATGCCACTT 
TCCCCAGACC TCAACCAGGC AACTCTCTCT CCAGACATCA GTCAGACATC CCTTCCTCTT 
GATCTCAGGC AGACATCACC TCCTCTAGAT CTCCATCAGA CATCCCACAC TTCTGAATTT 
AGTCAGTCAT TGCCTCTCCC AGAATTTGGT CAGACTTTCC CTTATGCAGA TCTTGGTCAG 
ATGCCATCTC CTCCACCACA CTCTCCACTA AATAACACTT TTATACCAAG AGAATTTAAT 
CCACTGGTCA TAGTAGGCCT CAGTAGAGAT GATGGAGATT ACATTGAAAT TATTCCAAGG 
CAGAAGGAAG AGAGCAGTGA AGAAGACTAT AGTGAATTTG ATTTTGTGGC TTATGATGAC 
CCTTACCAAA CTGATCTTAG GACAGACATC AACTCCTCCA GAAATCCTGA CAACATTGCA 
GCATGGTACC TTCGCAGCAA CAATGGAAAC AGAAAAAATT ATTACATTGC TGCTGAAGAA 
CTATCCTGGG ATTATTCAAA ATTTGCACAA AGTGAAGACA CTGATGATGT TTCAAAGGAC 
ACCATATACA AGAAAGTAGT TTTTCGAAAG TACCTTGATA GCACTTTTAA AAAGCTTGAT 
CCTCGGGGAG AGTATGAAGA GCATCTTGGC ATACTTGGTC CTATCATTAG AGCTGAAGTG 
GATGATGTTA TCCAAGTTCG TTTTAAAAAC TTAGCATCCA GACCATATTC TCTTCATGCC 
CATGGGCTTT CCTATGAAAA ATCATCAGAG GGAAAGACTT ATGAAGATGA CTCTCCTGAA 
TGGTTTAAGG AAGACAATGC TGTTCAGCCC AACAGCACTT ATACATATGT ATGGCACGCC 
ACTGAGCGAT CAGGGCCAGA AAATCCTGGC TCTGCCTGTC GGGCTTGGGC CTACTACTCA 
GCAGTGAACC CGGAAAAAGA CATCCACTCA GGCTTGATAG GACCCCTTCT GATCTGCCGA 
AAAGGAACAC TTCATAAGGA GACCAACATG CCTGTGGACA TGAGAGAATT TGTCCTACTT 
TTTATGGTCT TTGATGAAAA GAAGAGCTGG TACTATGAAA AGAAGCCCAT GAGGTCTTGG 
AGACGCACAT CCTCAGAAGT AAAAAACTCC CATGAGTTTC ATGCCATTAA TGGGATGATC 
TACAACTTGC CTGGCCTGAG AATGTACGAG CAAGAGTGGG TGAGGTTGCA CCTGCTGAAT 
GTAGGCGGCT CCCAGGACAT TCATGTGGTT CACTTTCATG GCCAGACCTT GCTGGAAAAT 
GGCACTCAAC AGTACCAGTT AGGGGTCTGG CCTCTTCTGC CTGGTTCATT TAAAACTCTT 
GAAATGAAGG CTTCAAAACC TGGCTGGTGG CTCCTAGACA CAGAAGTTGG AGAAAACCAG 
AGAGCAGGGA TGCAAACGCT ATTTCTCATC ACAGACAGAG ACTGTAAAAT GCCAATGGGA 
CTAAGCACTG GTCTCATAGC TGATTCACAG ATCAAGGCTT CTGAGTTTTG GGGTTATTGG 
GAACCCAAAT TAGCAAGACT AAACAATGGT GGATCATATA ATGCTTGGAT TGCAGAAAAA 
CTTTCAGTAG AATTTAACCC TAAACCTTGG ATCCAGGTAG ACATGCAAAG GGAAGTCCTG 
TTCACGGGGA TCCAGACCCA AGGTGCCAAA CACTACCTGA AGTCTTACTA CACCACCGAG 
TTCTTTGTGG CTTACAGCTC TGACCAGACA AACTGGCGGA TCTTCAAAGG GAACAGCACT 
AGGAACGTGA TGTATTTTAA TGGCAATTCA GATGCCTCTA CAATAAAAGA GAATCAGTTT 
GACCCACCTG TTGTGGCTAG ATATATTAGA ATCTCTCCAA CTAAATCCTA TAACAAACCT 
GCCCTTCGAT TGGAACTGCA AGGTTGCGAG GTTAACGGAT GCTCCACACC TCTGGGTATG 
GAAAGTGGAA AGATAGAAGA CAAGCAAATC ACAGCTTCCT CATTTAAAAA ATCTTGGTGG 
GGAGATTACT GGGAACCCTT CTGTGCCCGT CTTAATGCCC AGGGCCGTGT GAATGCCTGG 
CAAGCCAAGG CAAACAACAA CAATCAGTGG TTACAAATTG ATCTGCTCAA AATCAAGAAG 
ATAACAGCAA TTATAACACA GGGTTGCAAG TCTCTGTCCT CTGAAATGTA TGTGAAGAGC 
TACACCATCC AGTACAGTGA CCAGGGAGTT GAATGGAAAC CTTACAGAGA GAAATCCTCG 
TTGGTGGACA AGATTTTCGA AGGAAATAAT AATATCAAAG GACATGCAAA GAACTTTTTC 
AACCCACCAA TCATTTCCAG GTTTATACGC ATCATTCCTA AAACATGGAA TCAAAGCATT 
GCACTTCGCC TGGAACTCTT TGGCTGTGAT ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023972457.1
CDS	26..6841
Translation	MFPACPCLWVLVVLGSSWAGWGSLGAEAARLRQFYVAAQSISWNYLPESTNLSSNTFATSFKKIVYREYEAYFQKEKPRSRTSGLLGPTLYAEVGDIMKVHFKNKADKPLSIHPQGIKYSKFSEGASYPDHTFPVERMDDSVAPGQEYTYEWNISEDSGPTHDDPPCLTYIYYSYENLVHDFNSGLIGPLLICKKGTLTEDGIQKMFDKQHVLMFAVFDESKSWNQTSSLMYTVNGYVNGTMPDITVCVHDHISWHLIGMSSGPELFSIHFNGQVLEENHHKMSAITLVSATSTTANMTVSLEGKWIISSLIPKHFQAGMQAYIDIKNCAKKTRNPKKLTRDQRRHIKRWEYFIAAEEVIWDYAPIIPANMDKKYRSLHLDNFSNQIGKHYKKVVYKQYQDASFTKRLENPNVKEDGILGPIIRAQVRDTLKIMFKNMASRSYSIYPHGVTFSPYEDEVNSSFASGSNTMIKAVQPGETYTYKWNILESDEPMENDAQCLTRPYYSNVDITRDIASGLIGLVLICKSRSLDKRGIQRTADIEQQAVFAVFDENKSWYIEDNIYKFCENPEKVKRDDPKFYESNIMSTINGYVPESIPTLGFCFDDTVQWHFCSMGTQNDILTIHFTGHSFIYGKKHEDTLTLFPMRGESVTVTMDNVGTWMLTTMNSNPRSKKLWLRFRDVKCIREYDDDSYEIIYEPSGSTSKTTRKMRDSSEIKDKTNDADYDYQNTLALLLGIRSFRNSSSNQEEDEFNLTALALGNNSEFIPPSTNTTVGSNSSSPGNISRLIANNFAQLLKILPHSEATTAGPPLGHTSLDKNSVLNPPTAEHSSPYSEDPLYSDVTGISLLPFATEEFRNQEHAKRKRFKAGRGRAAKQRFSQMEFLARKTGRHLSKGNSSSSRIGPWENLPSDLLLLQQKDPSKILNGEWHLVSEKGSYEIIQDADEDKAVNKLPNSPQNDSRTWGENIPFKNKHGKQSGHPTFFVIRHKPLQGRQDGGNSRLKKGLFLIRTRRKKKEEKPAHHVPLSPRSFYPLRVEANTPSSDGRHNHSLLLHTSNETSLPTDLNQTFPSMNLSLIASLPDGDQNPPNDTIQTSSPPDLYQTVSPEEHYQTFPIQDSDPTHSTTVPSHRYPPPEPSQIPDYDLRNKAFPTDVSQTFPSLELEVWQTTTSLDLSQPSLYPDLSQTTLSPDPGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPVLSQETLSPDLSQMALSPHPGQETLSPDFSQTTLSPDLSQETLSPDLGQIALSPDLGQETLSPDLSQTTFSPDFSQTISPDLRQETLSPGLNQTALSPDLSQETLSPDLSQTTFSPDLSQETLSPDLSPMALSPDLGQETLSPDLSQTTLSPDLSQTTLSPDLGQETLSADLSQTTLSPDLGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPDLSQALSPDISQMVLSPDLSQMPLSPDLNQATLSPDISQTSLPLDLRQTSPPLDLHQTSHTSEFSQSLPLPEFGQTFPYADLGQMPSPPPHSPLNNTFIPREFNPLVIVGLSRDDGDYIEIIPRQKEESSEEDYSEFDFVAYDDPYQTDLRTDINSSRNPDNIAAWYLRSNNGNRKNYYIAAEELSWDYSKFAQSEDTDDVSKDTIYKKVVFRKYLDSTFKKLDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKTYEDDSPEWFKEDNAVQPNSTYTYVWHATERSGPENPGSACRAWAYYSAVNPEKDIHSGLIGPLLICRKGTLHKETNMPVDMREFVLLFMVFDEKKSWYYEKKPMRSWRRTSSEVKNSHEFHAINGMIYNLPGLRMYEQEWVRLHLLNVGGSQDIHVVHFHGQTLLENGTQQYQLGVWPLLPGSFKTLEMKASKPGWWLLDTEVGENQRAGMQTLFLITDRDCKMPMGLSTGLIADSQIKASEFWGYWEPKLARLNNGGSYNAWIAEKLSVEFNPKPWIQVDMQREVLFTGIQTQGAKHYLKSYYTTEFFVAYSSDQTNWRIFKGNSTRNVMYFNGNSDASTIKENQFDPPVVARYIRISPTKSYNKPALRLELQGCEVNGCSTPLGMESGKIEDKQITASSFKKSWWGDYWEPFCARLNAQGRVNAWQAKANNNNQWLQIDLLKIKKITAIITQGCKSLSSEMYVKSYTIQYSDQGVEWKPYREKSSLVDKIFEGNNNIKGHAKNFFNPPIISRFIRIIPKTWNQSIALRLELFGCDI

Target ORF information:

RefSeq Version	XM_024116689.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon coagulation factor V (F5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024116689.1

ORF Insert Sequence:

ATGTTCCCAG CTTGCCCTTG CCTCTGGGTC CTCGTGGTCC TGGGCTCCAG CTGGGCAGGC 
TGGGGGAGCC TAGGGGCTGA AGCAGCGCGG CTAAGGCAGT TCTACGTGGC TGCTCAGAGC 
ATCAGCTGGA ACTATCTCCC CGAGTCCACA AACCTTAGTT CGAATACTTT TGCAACCTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACGATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAGGCAGA CAAACCCTTA AGCATCCATC CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCTTC TTACCCTGAC CACACATTCC CTGTGGAGAG GATGGATGAT 
TCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGAATA TCAGTGAGGA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACATAT ATCTATTACT CCTATGAAAA TCTGGTACAC 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACTGAG 
GATGGGATAC AGAAGATGTT TGACAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCCTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGTCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATTCAT TTCAATGGCC AGGTCCTGGA AGAGAACCAT 
CATAAGATGT CAGCCATCAC TCTCGTCAGT GCTACGTCCA CAACTGCAAA CATGACTGTG 
AGCCTAGAGG GAAAGTGGAT CATATCTTCG CTCATCCCAA AACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GGAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AGTACAGATC TCTGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGCATCCT TCACCAAACG CCTGGAGAAT CCCAATGTCA AAGAAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTTAAAATCA TGTTCAAAAA TATGGCCAGC 
CGCAGCTACA GCATTTACCC TCATGGTGTG ACCTTCTCTC CTTATGAAGA TGAAGTCAAC 
TCTTCCTTCG CCTCAGGCAG TAACACCATG ATCAAAGCAG TTCAACCAGG GGAAACCTAT 
ACTTATAAGT GGAACATCCT AGAGTCTGAT GAACCCATGG AAAATGATGC TCAATGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACA TTGCCTCTGG GCTGATAGGG 
CTAGTTCTAA TTTGTAAGAG CAGATCCTTG GATAAGCGAG GCATACAGAG GACAGCAGAC 
ATCGAGCAAC AGGCTGTGTT TGCTGTGTTT GATGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAGGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGCAC TATCAATGGC TATGTGCCTG AGAGCATACC CACACTAGGA 
TTCTGCTTTG ATGACACCGT CCAGTGGCAC TTCTGCAGTA TGGGAACCCA GAATGACATT 
TTGACCATTC ACTTCACTGG GCACTCATTC ATCTATGGAA AGAAACATGA GGACACCTTG 
ACCCTCTTCC CCATGCGTGG GGAATCTGTG ACCGTCACAA TGGATAACGT TGGAACTTGG 
ATGTTAACTA CCATGAATTC CAATCCAAGA AGCAAAAAAC TGTGGCTGAG ATTCAGGGAT 
GTTAAGTGTA TCCGGGAGTA TGATGATGAC TCATATGAGA TTATATACGA ACCTTCAGGA 
TCTACATCCA AAACTACAAG GAAAATGCGT GATTCTTCAG AAATCAAAGA TAAAACTAAT 
GATGCTGACT ATGATTACCA GAACACACTG GCTTTATTAT TAGGAATCAG GTCATTCAGA 
AATTCATCAT CAAATCAGGA GGAAGATGAG TTCAATCTTA CTGCCCTGGC TCTGGGGAAC 
AACTCTGAAT TCATTCCTCC AAGTACCAAC ACAACTGTTG GTTCAAATTC TTCTTCCCCA 
GGTAACATTA GCAGGCTTAT TGCCAATAAC TTTGCACAAC TTTTGAAAAT TCTTCCTCAC 
TCAGAAGCCA CCACAGCTGG TCCCCCACTG GGACACACCA GCTTAGATAA GAACTCAGTT 
CTCAACCCTC CCACAGCAGA GCATTCCAGC CCTTATTCTG AAGATCCTCT ATACTCAGAT 
GTCACAGGGA TAAGCCTGCT TCCATTTGCT ACAGAAGAAT TCAGAAATCA AGAACATGCT 
AAACGTAAAA GATTCAAGGC AGGAAGAGGC CGAGCAGCAA AGCAAAGGTT CTCCCAGATG 
GAATTCCTAG CACGTAAAAC TGGTAGACAT TTAAGCAAAG GCAACTCTTC TTCTTCCAGA 
ATAGGGCCCT GGGAGAACCT TCCCAGTGAT CTGTTACTCT TGCAACAAAA GGATCCATCT 
AAGATTCTGA ATGGGGAATG GCATTTGGTT TCTGAGAAAG GCAGTTATGA AATAATCCAA 
GATGCTGATG AAGACAAGGC TGTTAATAAG CTGCCAAACA GCCCCCAGAA TGACTCAAGG 
ACTTGGGGAG AAAACATCCC TTTTAAAAAC AAGCATGGAA AGCAGAGTGG CCACCCAACG 
TTTTTTGTAA TTAGACATAA ACCTCTACAA GGAAGACAAG ATGGAGGAAA TAGTAGATTG 
AAGAAAGGCC TTTTTTTAAT TAGGACACGA AGAAAGAAAA AGGAAGAGAA GCCTGCACAC 
CATGTTCCTC TATCTCCAAG GAGTTTTTAC CCTCTAAGAG TAGAGGCCAA CACCCCATCT 
TCAGATGGGA GACATAATCA TTCATTGTTA CTCCATACAT CCAATGAAAC ATCTCTTCCC 
ACAGACCTCA ATCAGACATT CCCCTCTATG AACCTTAGCC TCATAGCCTC ACTTCCTGAT 
GGTGATCAAA ACCCCCCAAA TGACACCATT CAGACAAGCT CCCCTCCAGA TCTTTATCAG 
ACAGTGAGCC CAGAGGAACA CTATCAAACA TTCCCTATTC AAGACTCTGA TCCAACGCAC 
TCCACTACAG TCCCCAGTCA CAGATATCCT CCTCCAGAGC CCAGCCAGAT ACCCGACTAT 
GACCTAAGAA ACAAGGCCTT TCCTACTGAT GTTAGTCAAA CGTTCCCTTC CTTGGAACTT 
GAAGTCTGGC AGACAACCAC CTCTCTAGAC CTCAGTCAGC CATCCCTCTA TCCAGATCTC 
AGCCAGACCA CCCTTTCTCC AGACCCTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT TGGCCAGGAG ACTCTCTCTC CAGACCTCAG CCAGACAACA 
CTTTCCCCAG ACCTTGGCCA GGAGACTCTC TCTCCAGACC TCAGCCAGAC AACACTTTCC 
CCAGTTCTTA GCCAGGAGAC TCTCTCTCCA GACCTCAGCC AGATGGCCCT TTCCCCACAC 
CCTGGCCAGG AGACGCTTTC TCCAGACTTC AGCCAGACAA CACTTTCCCC AGACCTTAGT 
CAGGAGACTC TCTCTCCAGA CCTCGGACAG ATAGCCCTTT CCCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACA TTTTCCCCAG ACTTCAGCCA GACTATCTCT 
CCAGACCTCA GACAGGAGAC TCTCTCTCCA GGCCTCAACC AGACAGCCCT TTCCCCAGAC 
CTTAGCCAGG AGACTCTCTC TCCAGACCTC AGCCAGACAA CATTTTCCCC AGACCTTAGC 
CAGGAGACTC TCTCTCCAGA CCTCAGCCCG ATGGCCCTTT CGCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACG CTTTCCCCAG ACCTCAGCCA GACAACACTT 
TCCCCAGACC TTGGCCAGGA GACTCTCTCT GCAGACCTCA GCCAGACAAC ACTTTCCCCA 
GACCTCGGCC AGGAGACTCT CTCTCCAGAC CTCAGCCAGA CAACACTTTC CCCAGACCTT 
GGCCAGGAGA CTCTCTCTCC AGACCTCAGC CAGACAACAC TTTCCCCAGA CCTCAGCCAG 
GCTCTCTCTC CAGACATCAG CCAGATGGTC CTTTCCCCAG ACCTCAGTCA GATGCCACTT 
TCCCCAGACC TCAACCAGGC AACTCTCTCT CCAGACATCA GTCAGACATC CCTTCCTCTT 
GATCTCAGGC AGACATCACC TCCTCTAGAT CTCCATCAGA CATCCCACAC TTCTGAATTT 
AGTCAGTCAT TGCCTCTCCC AGAATTTGGT CAGACTTTCC CTTATGCAGA TCTTGGTCAG 
ATGCCATCTC CTCCACCACA CTCTCCACTA AATAACACTT TTATACCAAG AGAATTTAAT 
CCACTGGTCA TAGTAGGCCT CAGTAGAGAT GATGGAGATT ACATTGAAAT TATTCCAAGG 
CAGAAGGAAG AGAGCAGTGA AGAAGACTAT AGTGAATTTG ATTTTGTGGC TTATGATGAC 
CCTTACCAAA CTGATCTTAG GACAGACATC AACTCCTCCA GAAATCCTGA CAACATTGCA 
GCATGGTACC TTCGCAGCAA CAATGGAAAC AGAAAAAATT ATTACATTGC TGCTGAAGAA 
CTATCCTGGG ATTATTCAAA ATTTGCACAA AGTGAAGACA CTGATGATGT TTCAAAGGAC 
ACCATATACA AGAAAGTAGT TTTTCGAAAG TACCTTGATA GCACTTTTAA AAAGCTTGAT 
CCTCGGGGAG AGTATGAAGA GCATCTTGGC ATACTTGGTC CTATCATTAG AGCTGAAGTG 
GATGATGTTA TCCAAGTTCG TTTTAAAAAC TTAGCATCCA GACCATATTC TCTTCATGCC 
CATGGGCTTT CCTATGAAAA ATCATCAGAG GGAAAGACTT ATGAAGATGA CTCTCCTGAA 
TGGTTTAAGG AAGACAATGC TGTTCAGCCC AACAGCACTT ATACATATGT ATGGCACGCC 
ACTGAGCGAT CAGGGCCAGA AAATCCTGGC TCTGCCTGTC GGGCTTGGGC CTACTACTCA 
GCAGTGAACC CGGAAAAAGA CATCCACTCA GGCTTGATAG GACCCCTTCT GATCTGCCGA 
AAAGGAACAC TTCATAAGGA GACCAACATG CCTGTGGACA TGAGAGAATT TGTCCTACTT 
TTTATGGTCT TTGATGAAAA GAAGAGCTGG TACTATGAAA AGAAGCCCAT GAGGTCTTGG 
AGACGCACAT CCTCAGAAGT AAAAAACTCC CATGAGTTTC ATGCCATTAA TGGGATGATC 
TACAACTTGC CTGGCCTGAG AATGTACGAG CAAGAGTGGG TGAGGTTGCA CCTGCTGAAT 
GTAGGCGGCT CCCAGGACAT TCATGTGGTT CACTTTCATG GCCAGACCTT GCTGGAAAAT 
GGCACTCAAC AGTACCAGTT AGGGGTCTGG CCTCTTCTGC CTGGTTCATT TAAAACTCTT 
GAAATGAAGG CTTCAAAACC TGGCTGGTGG CTCCTAGACA CAGAAGTTGG AGAAAACCAG 
AGAGCAGGGA TGCAAACGCT ATTTCTCATC ACAGACAGAG ACTGTAAAAT GCCAATGGGA 
CTAAGCACTG GTCTCATAGC TGATTCACAG ATCAAGGCTT CTGAGTTTTG GGGTTATTGG 
GAACCCAAAT TAGCAAGACT AAACAATGGT GGATCATATA ATGCTTGGAT TGCAGAAAAA 
CTTTCAGTAG AATTTAACCC TAAACCTTGG ATCCAGGTAG ACATGCAAAG GGAAGTCCTG 
TTCACGGGGA TCCAGACCCA AGGTGCCAAA CACTACCTGA AGTCTTACTA CACCACCGAG 
TTCTTTGTGG CTTACAGCTC TGACCAGACA AACTGGCGGA TCTTCAAAGG GAACAGCACT 
AGGAACGTGA TGTATTTTAA TGGCAATTCA GATGCCTCTA CAATAAAAGA GAATCAGTTT 
GACCCACCTG TTGTGGCTAG ATATATTAGA ATCTCTCCAA CTAAATCCTA TAACAAACCT 
GCCCTTCGAT TGGAACTGCA AGGTTGCGAG GTTAACGGAT GCTCCACACC TCTGGGTATG 
GAAAGTGGAA AGATAGAAGA CAAGCAAATC ACAGCTTCCT CATTTAAAAA ATCTTGGTGG 
GGAGATTACT GGGAACCCTT CTGTGCCCGT CTTAATGCCC AGGGCCGTGT GAATGCCTGG 
CAAGCCAAGG CAAACAACAA CAATCAGTGG TTACAAATTG ATCTGCTCAA AATCAAGAAG 
ATAACAGCAA TTATAACACA GGGTTGCAAG TCTCTGTCCT CTGAAATGTA TGTGAAGAGC 
TACACCATCC AGTACAGTGA CCAGGGAGTT GAATGGAAAC CTTACAGAGA GAAATCCTCG 
TTGGTGGACA AGATTTTCGA AGGAAATAAT AATATCAAAG GACATGCAAA GAACTTTTTC 
AACCCACCAA TCATTTCCAG GTTTATACGC ATCATTCCTA AAACATGGAA TCAAAGCATT 
GCACTTCGCC TGGAACTCTT TGGCTGTGAT ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F5 ( XM_024116690.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023972458.1 Physeter catodon coagulation factor V (F5), transcript variant X2, mRNA.

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CloneID	OPh305668
Clone ID Related Accession (Same CDS sequence)	XM_024116690.1
Accession Version	XM_024116690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	coagulation factor V isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873626.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAG CTTGCCCTTG CCTCTGGGTC CTCGTGGTCC TGGGCTCCAG CTGGGCAGGC 
TGGGGGAGCC TAGGGGCTGA AGCAGCGCGG CTAAGGCAGT TCTACGTGGC TGCTCAGAGC 
ATCAGCTGGA ACTATCTCCC CGAGTCCACA AACCTTAGTT CGAATACTTT TGCAACCTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACGATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAGGCAGA CAAACCCTTA AGCATCCATC CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCTTC TTACCCTGAC CACACATTCC CTGTGGAGAG GATGGATGAT 
TCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGAATA TCAGTGAGGA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACATAT ATCTATTACT CCTATGAAAA TCTGGTACAC 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACTGAG 
GATGGGATAC AGAAGATGTT TGACAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCCTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGTCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATTCAT TTCAATGGCC AGGTCCTGGA AGAGAACCAT 
CATAAGATGT CAGCCATCAC TCTCGTCAGT GCTACGTCCA CAACTGCAAA CATGACTGTG 
AGCCTAGAGG GAAAGTGGAT CATATCTTCG CTCATCCCAA AACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GGAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AGTACAGATC TCTGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGCATCCT TCACCAAACG CCTGGAGAAT CCCAATGTCA AAGAAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTTAAAATCA TGTTCAAAAA TATGGCCAGC 
CGCAGCTACA GCATTTACCC TCATGGTGTG ACCTTCTCTC CTTATGAAGA TGAAGTCAAC 
TCTTCCTTCG CCTCAGGCAG TAACACCATG ATCAAAGCAG TTCAACCAGG GGAAACCTAT 
ACTTATAAGT GGAACATCCT AGAGTCTGAT GAACCCATGG AAAATGATGC TCAATGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACA TTGCCTCTGG GCTGATAGGG 
CTAGTTCTAA TTTGTAAGAG CAGATCCTTG GATAAGCGAG GCATACAGAG GACAGCAGAC 
ATCGAGCAAC AGGCTGTGTT TGCTGTGTTT GATGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAGGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGCAC TATCAATGGC TATGTGCCTG AGAGCATACC CACACTAGGA 
TTCTGCTTTG ATGACACCGT CCAGTGGCAC TTCTGCAGTA TGGGAACCCA GAATGACATT 
TTGACCATTC ACTTCACTGG GCACTCATTC ATCTATGGAA AGAAACATGA GGACACCTTG 
ACCCTCTTCC CCATGCGTGG GGAATCTGTG ACCGTCACAA TGGATAACGT TGGAACTTGG 
ATGTTAACTA CCATGAATTC CAATCCAAGA AGCAAAAAAC TGTGGCTGAG ATTCAGGGAT 
GTTAAGTGTA TCCGGGAGTA TGATGATGAC TCATATGAGA TTATATACGA ACCTTCAGGA 
TCTACATCCA AAACTACAAG GAAAATGCGT GATTCTTCAG AAATCAAAGA TAAAACTAAT 
GATGCTGACT ATGATTACCA GAACACACTG GCTTTATTAT TAGGAATCAG GTCATTCAGA 
AATTCATCAT CAAATCAGGA GGAAGATGAG TTCAATCTTA CTGCCCTGGC TCTGGGGAAC 
AACTCTGAAT TCATTCCTCC AAGTACCAAC ACAACTGTTG GTTCAAATTC TTCTTCCCCA 
GGTAACATTA GCAGGCTTAT TGCCAATAAC TTTGCACAAC TTTTGAAAAT TCTTCCTCAC 
TCAGAAGCCA CCACAGCTGG TCCCCCACTG GGACACACCA GCTTAGATAA GAACTCAGTT 
CTCAACCCTC CCACAGCAGA GCATTCCAGC CCTTATTCTG AAGATCCTCT ATACTCAGAT 
GTCACAGGGA TAAGCCTGCT TCCATTTGCT ACAGAAGAAT TCAGAAATCA AGAACATGCT 
AAACGTAAAA GATTCAAGGC AGGAAGAGGC CGAGCAGCAA AGCAAAGGTT CTCCCAGATG 
GAATTCCTAG CACGTAAAAC TGGTAGACAT TTAAGCAAAG GCAACTCTTC TTCTTCCAGA 
ATAGGGCCCT GGGAGAACCT TCCCAGTGAT CTGTTACTCT TGCAACAAAA GGATCCATCT 
AAGATTCTGA ATGGGGAATG GCATTTGGTT TCTGAGAAAG GCAGTTATGA AATAATCCAA 
GATGCTGATG AAGACAAGGC TGTTAATAAG CTGCCAAACA GCCCCCAGAA TGACTCAAGG 
ACTTGGGGAG AAAACATCCC TTTTAAAAAC AAGCATGGAA AGCAGAGTGG CCACCCAACG 
TTTTTTGTAA TTAGACATAA ACCTCTACAA GGAAGACAAG ATGGAGGAAA TAGTAGATTG 
AAGAAAGGCC TTTTTTTAAT TAGGACACGA AGAAAGAAAA AGGAAGAGAA GCCTGCACAC 
CATGTTCCTC TATCTCCAAG GAGTTTTTAC CCTCTAAGAG TAGAGGCCAA CACCCCATCT 
TCAGATGGGA GACATAATCA TTCATTGTTA CTCCATACAT CCAATGAAAC ATCTCTTCCC 
ACAGACCTCA ATCAGACATT CCCCTCTATG AACCTTAGCC TCATAGCCTC ACTTCCTGAT 
GGTGATCAAA ACCCCCCAAA TGACACCATT CAGACAAGCT CCCCTCCAGA TCTTTATCAG 
ACAGTGAGCC CAGAGGAACA CTATCAAACA TTCCCTATTC AAGACTCTGA TCCAACGCAC 
TCCACTACAG TCCCCAGTCA CAGATATCCT CCTCCAGAGC CCAGCCAGAT ACCCGACTAT 
GACCTAAGAA ACAAGGCCTT TCCTACTGAT GTTAGTCAAA CGTTCCCTTC CTTGGAACTT 
GAAGTCTGGC AGACAACCAC CTCTCTAGAC CTCAGTCAGC CATCCCTCTA TCCAGATCTC 
AGCCAGACCA CCCTTTCTCC AGACCCTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT TGGCCAGGAG ACTCTCTCTC CAGACCTCAG CCAGACAACA 
CTTTCCCCAG ACCTTGGCCA GGAGACTCTC TCTCCAGACC TCAGCCAGAC AACACTTTCC 
CCAGTTCTTA GCCAGGAGAC TCTCTCTCCA GACCTCAGCC AGATGGCCCT TTCCCCACAC 
CCTGGCCAGG AGACGCTTTC TCCAGACTTC AGCCAGACAA CACTTTCCCC AGACCTTAGT 
CAGGAGACTC TCTCTCCAGA CCTCGGACAG ATAGCCCTTT CCCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACA TTTTCCCCAG ACTTCAGCCA GACTATCTCT 
CCAGACCTCA GACAGGAGAC TCTCTCTCCA GGCCTCAACC AGACAGCCCT TTCCCCAGAC 
CTTAGCCAGG AGACTCTCTC TCCAGACCTC AGCCAGACAA CATTTTCCCC AGACCTTAGC 
CAGGAGACTC TCTCTCCAGA CCTCAGCCCG ATGGCCCTTT CGCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACG CTTTCCCCAG ACCTCAGCCA GGAGACTCTC 
TCTGCAGACC TCAGCCAGAC AACACTTTCC CCAGACCTTG GCCAGGAGAC TCTCTCTGCA 
GACCTCAGCC AGACAACACT TTCCCCAGAC CTCGGCCAGG AGACTCTCTC TCCAGACCTC 
AGCCAGACAA CACTTTCCCC AGACCTTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT CAGCCAGGCT CTCTCTCCAG ACATCAGCCA GATGGTCCTT 
TCCCCAGACC TCAGTCAGAT GCCACTTTCC CCAGACCTCA ACCAGGCAAC TCTCTCTCCA 
GACATCAGTC AGACATCCCT TCCTCTTGAT CTCAGGCAGA CATCACCTCC TCTAGATCTC 
CATCAGACAT CCCACACTTC TGAATTTAGT CAGTCATTGC CTCTCCCAGA ATTTGGTCAG 
ACTTTCCCTT ATGCAGATCT TGGTCAGATG CCATCTCCTC CACCACACTC TCCACTAAAT 
AACACTTTTA TACCAAGAGA ATTTAATCCA CTGGTCATAG TAGGCCTCAG TAGAGATGAT 
GGAGATTACA TTGAAATTAT TCCAAGGCAG AAGGAAGAGA GCAGTGAAGA AGACTATAGT 
GAATTTGATT TTGTGGCTTA TGATGACCCT TACCAAACTG ATCTTAGGAC AGACATCAAC 
TCCTCCAGAA ATCCTGACAA CATTGCAGCA TGGTACCTTC GCAGCAACAA TGGAAACAGA 
AAAAATTATT ACATTGCTGC TGAAGAACTA TCCTGGGATT ATTCAAAATT TGCACAAAGT 
GAAGACACTG ATGATGTTTC AAAGGACACC ATATACAAGA AAGTAGTTTT TCGAAAGTAC 
CTTGATAGCA CTTTTAAAAA GCTTGATCCT CGGGGAGAGT ATGAAGAGCA TCTTGGCATA 
CTTGGTCCTA TCATTAGAGC TGAAGTGGAT GATGTTATCC AAGTTCGTTT TAAAAACTTA 
GCATCCAGAC CATATTCTCT TCATGCCCAT GGGCTTTCCT ATGAAAAATC ATCAGAGGGA 
AAGACTTATG AAGATGACTC TCCTGAATGG TTTAAGGAAG ACAATGCTGT TCAGCCCAAC 
AGCACTTATA CATATGTATG GCACGCCACT GAGCGATCAG GGCCAGAAAA TCCTGGCTCT 
GCCTGTCGGG CTTGGGCCTA CTACTCAGCA GTGAACCCGG AAAAAGACAT CCACTCAGGC 
TTGATAGGAC CCCTTCTGAT CTGCCGAAAA GGAACACTTC ATAAGGAGAC CAACATGCCT 
GTGGACATGA GAGAATTTGT CCTACTTTTT ATGGTCTTTG ATGAAAAGAA GAGCTGGTAC 
TATGAAAAGA AGCCCATGAG GTCTTGGAGA CGCACATCCT CAGAAGTAAA AAACTCCCAT 
GAGTTTCATG CCATTAATGG GATGATCTAC AACTTGCCTG GCCTGAGAAT GTACGAGCAA 
GAGTGGGTGA GGTTGCACCT GCTGAATGTA GGCGGCTCCC AGGACATTCA TGTGGTTCAC 
TTTCATGGCC AGACCTTGCT GGAAAATGGC ACTCAACAGT ACCAGTTAGG GGTCTGGCCT 
CTTCTGCCTG GTTCATTTAA AACTCTTGAA ATGAAGGCTT CAAAACCTGG CTGGTGGCTC 
CTAGACACAG AAGTTGGAGA AAACCAGAGA GCAGGGATGC AAACGCTATT TCTCATCACA 
GACAGAGACT GTAAAATGCC AATGGGACTA AGCACTGGTC TCATAGCTGA TTCACAGATC 
AAGGCTTCTG AGTTTTGGGG TTATTGGGAA CCCAAATTAG CAAGACTAAA CAATGGTGGA 
TCATATAATG CTTGGATTGC AGAAAAACTT TCAGTAGAAT TTAACCCTAA ACCTTGGATC 
CAGGTAGACA TGCAAAGGGA AGTCCTGTTC ACGGGGATCC AGACCCAAGG TGCCAAACAC 
TACCTGAAGT CTTACTACAC CACCGAGTTC TTTGTGGCTT ACAGCTCTGA CCAGACAAAC 
TGGCGGATCT TCAAAGGGAA CAGCACTAGG AACGTGATGT ATTTTAATGG CAATTCAGAT 
GCCTCTACAA TAAAAGAGAA TCAGTTTGAC CCACCTGTTG TGGCTAGATA TATTAGAATC 
TCTCCAACTA AATCCTATAA CAAACCTGCC CTTCGATTGG AACTGCAAGG TTGCGAGGTT 
AACGGATGCT CCACACCTCT GGGTATGGAA AGTGGAAAGA TAGAAGACAA GCAAATCACA 
GCTTCCTCAT TTAAAAAATC TTGGTGGGGA GATTACTGGG AACCCTTCTG TGCCCGTCTT 
AATGCCCAGG GCCGTGTGAA TGCCTGGCAA GCCAAGGCAA ACAACAACAA TCAGTGGTTA 
CAAATTGATC TGCTCAAAAT CAAGAAGATA ACAGCAATTA TAACACAGGG TTGCAAGTCT 
CTGTCCTCTG AAATGTATGT GAAGAGCTAC ACCATCCAGT ACAGTGACCA GGGAGTTGAA 
TGGAAACCTT ACAGAGAGAA ATCCTCGTTG GTGGACAAGA TTTTCGAAGG AAATAATAAT 
ATCAAAGGAC ATGCAAAGAA CTTTTTCAAC CCACCAATCA TTTCCAGGTT TATACGCATC 
ATTCCTAAAA CATGGAATCA AAGCATTGCA CTTCGCCTGG AACTCTTTGG CTGTGATATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023972458.1
CDS	26..6868
Translation	MFPACPCLWVLVVLGSSWAGWGSLGAEAARLRQFYVAAQSISWNYLPESTNLSSNTFATSFKKIVYREYEAYFQKEKPRSRTSGLLGPTLYAEVGDIMKVHFKNKADKPLSIHPQGIKYSKFSEGASYPDHTFPVERMDDSVAPGQEYTYEWNISEDSGPTHDDPPCLTYIYYSYENLVHDFNSGLIGPLLICKKGTLTEDGIQKMFDKQHVLMFAVFDESKSWNQTSSLMYTVNGYVNGTMPDITVCVHDHISWHLIGMSSGPELFSIHFNGQVLEENHHKMSAITLVSATSTTANMTVSLEGKWIISSLIPKHFQAGMQAYIDIKNCAKKTRNPKKLTRDQRRHIKRWEYFIAAEEVIWDYAPIIPANMDKKYRSLHLDNFSNQIGKHYKKVVYKQYQDASFTKRLENPNVKEDGILGPIIRAQVRDTLKIMFKNMASRSYSIYPHGVTFSPYEDEVNSSFASGSNTMIKAVQPGETYTYKWNILESDEPMENDAQCLTRPYYSNVDITRDIASGLIGLVLICKSRSLDKRGIQRTADIEQQAVFAVFDENKSWYIEDNIYKFCENPEKVKRDDPKFYESNIMSTINGYVPESIPTLGFCFDDTVQWHFCSMGTQNDILTIHFTGHSFIYGKKHEDTLTLFPMRGESVTVTMDNVGTWMLTTMNSNPRSKKLWLRFRDVKCIREYDDDSYEIIYEPSGSTSKTTRKMRDSSEIKDKTNDADYDYQNTLALLLGIRSFRNSSSNQEEDEFNLTALALGNNSEFIPPSTNTTVGSNSSSPGNISRLIANNFAQLLKILPHSEATTAGPPLGHTSLDKNSVLNPPTAEHSSPYSEDPLYSDVTGISLLPFATEEFRNQEHAKRKRFKAGRGRAAKQRFSQMEFLARKTGRHLSKGNSSSSRIGPWENLPSDLLLLQQKDPSKILNGEWHLVSEKGSYEIIQDADEDKAVNKLPNSPQNDSRTWGENIPFKNKHGKQSGHPTFFVIRHKPLQGRQDGGNSRLKKGLFLIRTRRKKKEEKPAHHVPLSPRSFYPLRVEANTPSSDGRHNHSLLLHTSNETSLPTDLNQTFPSMNLSLIASLPDGDQNPPNDTIQTSSPPDLYQTVSPEEHYQTFPIQDSDPTHSTTVPSHRYPPPEPSQIPDYDLRNKAFPTDVSQTFPSLELEVWQTTTSLDLSQPSLYPDLSQTTLSPDPGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPVLSQETLSPDLSQMALSPHPGQETLSPDFSQTTLSPDLSQETLSPDLGQIALSPDLGQETLSPDLSQTTFSPDFSQTISPDLRQETLSPGLNQTALSPDLSQETLSPDLSQTTFSPDLSQETLSPDLSPMALSPDLGQETLSPDLSQTTLSPDLSQETLSADLSQTTLSPDLGQETLSADLSQTTLSPDLGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPDLSQALSPDISQMVLSPDLSQMPLSPDLNQATLSPDISQTSLPLDLRQTSPPLDLHQTSHTSEFSQSLPLPEFGQTFPYADLGQMPSPPPHSPLNNTFIPREFNPLVIVGLSRDDGDYIEIIPRQKEESSEEDYSEFDFVAYDDPYQTDLRTDINSSRNPDNIAAWYLRSNNGNRKNYYIAAEELSWDYSKFAQSEDTDDVSKDTIYKKVVFRKYLDSTFKKLDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKTYEDDSPEWFKEDNAVQPNSTYTYVWHATERSGPENPGSACRAWAYYSAVNPEKDIHSGLIGPLLICRKGTLHKETNMPVDMREFVLLFMVFDEKKSWYYEKKPMRSWRRTSSEVKNSHEFHAINGMIYNLPGLRMYEQEWVRLHLLNVGGSQDIHVVHFHGQTLLENGTQQYQLGVWPLLPGSFKTLEMKASKPGWWLLDTEVGENQRAGMQTLFLITDRDCKMPMGLSTGLIADSQIKASEFWGYWEPKLARLNNGGSYNAWIAEKLSVEFNPKPWIQVDMQREVLFTGIQTQGAKHYLKSYYTTEFFVAYSSDQTNWRIFKGNSTRNVMYFNGNSDASTIKENQFDPPVVARYIRISPTKSYNKPALRLELQGCEVNGCSTPLGMESGKIEDKQITASSFKKSWWGDYWEPFCARLNAQGRVNAWQAKANNNNQWLQIDLLKIKKITAIITQGCKSLSSEMYVKSYTIQYSDQGVEWKPYREKSSLVDKIFEGNNNIKGHAKNFFNPPIISRFIRIIPKTWNQSIALRLELFGCDI

Target ORF information:

RefSeq Version	XM_024116690.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon coagulation factor V (F5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024116690.1

ORF Insert Sequence:

ATGTTCCCAG CTTGCCCTTG CCTCTGGGTC CTCGTGGTCC TGGGCTCCAG CTGGGCAGGC 
TGGGGGAGCC TAGGGGCTGA AGCAGCGCGG CTAAGGCAGT TCTACGTGGC TGCTCAGAGC 
ATCAGCTGGA ACTATCTCCC CGAGTCCACA AACCTTAGTT CGAATACTTT TGCAACCTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACGATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAGGCAGA CAAACCCTTA AGCATCCATC CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCTTC TTACCCTGAC CACACATTCC CTGTGGAGAG GATGGATGAT 
TCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGAATA TCAGTGAGGA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACATAT ATCTATTACT CCTATGAAAA TCTGGTACAC 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACTGAG 
GATGGGATAC AGAAGATGTT TGACAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCCTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGTCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATTCAT TTCAATGGCC AGGTCCTGGA AGAGAACCAT 
CATAAGATGT CAGCCATCAC TCTCGTCAGT GCTACGTCCA CAACTGCAAA CATGACTGTG 
AGCCTAGAGG GAAAGTGGAT CATATCTTCG CTCATCCCAA AACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GGAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AGTACAGATC TCTGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGCATCCT TCACCAAACG CCTGGAGAAT CCCAATGTCA AAGAAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTTAAAATCA TGTTCAAAAA TATGGCCAGC 
CGCAGCTACA GCATTTACCC TCATGGTGTG ACCTTCTCTC CTTATGAAGA TGAAGTCAAC 
TCTTCCTTCG CCTCAGGCAG TAACACCATG ATCAAAGCAG TTCAACCAGG GGAAACCTAT 
ACTTATAAGT GGAACATCCT AGAGTCTGAT GAACCCATGG AAAATGATGC TCAATGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACA TTGCCTCTGG GCTGATAGGG 
CTAGTTCTAA TTTGTAAGAG CAGATCCTTG GATAAGCGAG GCATACAGAG GACAGCAGAC 
ATCGAGCAAC AGGCTGTGTT TGCTGTGTTT GATGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAGGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGCAC TATCAATGGC TATGTGCCTG AGAGCATACC CACACTAGGA 
TTCTGCTTTG ATGACACCGT CCAGTGGCAC TTCTGCAGTA TGGGAACCCA GAATGACATT 
TTGACCATTC ACTTCACTGG GCACTCATTC ATCTATGGAA AGAAACATGA GGACACCTTG 
ACCCTCTTCC CCATGCGTGG GGAATCTGTG ACCGTCACAA TGGATAACGT TGGAACTTGG 
ATGTTAACTA CCATGAATTC CAATCCAAGA AGCAAAAAAC TGTGGCTGAG ATTCAGGGAT 
GTTAAGTGTA TCCGGGAGTA TGATGATGAC TCATATGAGA TTATATACGA ACCTTCAGGA 
TCTACATCCA AAACTACAAG GAAAATGCGT GATTCTTCAG AAATCAAAGA TAAAACTAAT 
GATGCTGACT ATGATTACCA GAACACACTG GCTTTATTAT TAGGAATCAG GTCATTCAGA 
AATTCATCAT CAAATCAGGA GGAAGATGAG TTCAATCTTA CTGCCCTGGC TCTGGGGAAC 
AACTCTGAAT TCATTCCTCC AAGTACCAAC ACAACTGTTG GTTCAAATTC TTCTTCCCCA 
GGTAACATTA GCAGGCTTAT TGCCAATAAC TTTGCACAAC TTTTGAAAAT TCTTCCTCAC 
TCAGAAGCCA CCACAGCTGG TCCCCCACTG GGACACACCA GCTTAGATAA GAACTCAGTT 
CTCAACCCTC CCACAGCAGA GCATTCCAGC CCTTATTCTG AAGATCCTCT ATACTCAGAT 
GTCACAGGGA TAAGCCTGCT TCCATTTGCT ACAGAAGAAT TCAGAAATCA AGAACATGCT 
AAACGTAAAA GATTCAAGGC AGGAAGAGGC CGAGCAGCAA AGCAAAGGTT CTCCCAGATG 
GAATTCCTAG CACGTAAAAC TGGTAGACAT TTAAGCAAAG GCAACTCTTC TTCTTCCAGA 
ATAGGGCCCT GGGAGAACCT TCCCAGTGAT CTGTTACTCT TGCAACAAAA GGATCCATCT 
AAGATTCTGA ATGGGGAATG GCATTTGGTT TCTGAGAAAG GCAGTTATGA AATAATCCAA 
GATGCTGATG AAGACAAGGC TGTTAATAAG CTGCCAAACA GCCCCCAGAA TGACTCAAGG 
ACTTGGGGAG AAAACATCCC TTTTAAAAAC AAGCATGGAA AGCAGAGTGG CCACCCAACG 
TTTTTTGTAA TTAGACATAA ACCTCTACAA GGAAGACAAG ATGGAGGAAA TAGTAGATTG 
AAGAAAGGCC TTTTTTTAAT TAGGACACGA AGAAAGAAAA AGGAAGAGAA GCCTGCACAC 
CATGTTCCTC TATCTCCAAG GAGTTTTTAC CCTCTAAGAG TAGAGGCCAA CACCCCATCT 
TCAGATGGGA GACATAATCA TTCATTGTTA CTCCATACAT CCAATGAAAC ATCTCTTCCC 
ACAGACCTCA ATCAGACATT CCCCTCTATG AACCTTAGCC TCATAGCCTC ACTTCCTGAT 
GGTGATCAAA ACCCCCCAAA TGACACCATT CAGACAAGCT CCCCTCCAGA TCTTTATCAG 
ACAGTGAGCC CAGAGGAACA CTATCAAACA TTCCCTATTC AAGACTCTGA TCCAACGCAC 
TCCACTACAG TCCCCAGTCA CAGATATCCT CCTCCAGAGC CCAGCCAGAT ACCCGACTAT 
GACCTAAGAA ACAAGGCCTT TCCTACTGAT GTTAGTCAAA CGTTCCCTTC CTTGGAACTT 
GAAGTCTGGC AGACAACCAC CTCTCTAGAC CTCAGTCAGC CATCCCTCTA TCCAGATCTC 
AGCCAGACCA CCCTTTCTCC AGACCCTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT TGGCCAGGAG ACTCTCTCTC CAGACCTCAG CCAGACAACA 
CTTTCCCCAG ACCTTGGCCA GGAGACTCTC TCTCCAGACC TCAGCCAGAC AACACTTTCC 
CCAGTTCTTA GCCAGGAGAC TCTCTCTCCA GACCTCAGCC AGATGGCCCT TTCCCCACAC 
CCTGGCCAGG AGACGCTTTC TCCAGACTTC AGCCAGACAA CACTTTCCCC AGACCTTAGT 
CAGGAGACTC TCTCTCCAGA CCTCGGACAG ATAGCCCTTT CCCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACA TTTTCCCCAG ACTTCAGCCA GACTATCTCT 
CCAGACCTCA GACAGGAGAC TCTCTCTCCA GGCCTCAACC AGACAGCCCT TTCCCCAGAC 
CTTAGCCAGG AGACTCTCTC TCCAGACCTC AGCCAGACAA CATTTTCCCC AGACCTTAGC 
CAGGAGACTC TCTCTCCAGA CCTCAGCCCG ATGGCCCTTT CGCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACG CTTTCCCCAG ACCTCAGCCA GGAGACTCTC 
TCTGCAGACC TCAGCCAGAC AACACTTTCC CCAGACCTTG GCCAGGAGAC TCTCTCTGCA 
GACCTCAGCC AGACAACACT TTCCCCAGAC CTCGGCCAGG AGACTCTCTC TCCAGACCTC 
AGCCAGACAA CACTTTCCCC AGACCTTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT CAGCCAGGCT CTCTCTCCAG ACATCAGCCA GATGGTCCTT 
TCCCCAGACC TCAGTCAGAT GCCACTTTCC CCAGACCTCA ACCAGGCAAC TCTCTCTCCA 
GACATCAGTC AGACATCCCT TCCTCTTGAT CTCAGGCAGA CATCACCTCC TCTAGATCTC 
CATCAGACAT CCCACACTTC TGAATTTAGT CAGTCATTGC CTCTCCCAGA ATTTGGTCAG 
ACTTTCCCTT ATGCAGATCT TGGTCAGATG CCATCTCCTC CACCACACTC TCCACTAAAT 
AACACTTTTA TACCAAGAGA ATTTAATCCA CTGGTCATAG TAGGCCTCAG TAGAGATGAT 
GGAGATTACA TTGAAATTAT TCCAAGGCAG AAGGAAGAGA GCAGTGAAGA AGACTATAGT 
GAATTTGATT TTGTGGCTTA TGATGACCCT TACCAAACTG ATCTTAGGAC AGACATCAAC 
TCCTCCAGAA ATCCTGACAA CATTGCAGCA TGGTACCTTC GCAGCAACAA TGGAAACAGA 
AAAAATTATT ACATTGCTGC TGAAGAACTA TCCTGGGATT ATTCAAAATT TGCACAAAGT 
GAAGACACTG ATGATGTTTC AAAGGACACC ATATACAAGA AAGTAGTTTT TCGAAAGTAC 
CTTGATAGCA CTTTTAAAAA GCTTGATCCT CGGGGAGAGT ATGAAGAGCA TCTTGGCATA 
CTTGGTCCTA TCATTAGAGC TGAAGTGGAT GATGTTATCC AAGTTCGTTT TAAAAACTTA 
GCATCCAGAC CATATTCTCT TCATGCCCAT GGGCTTTCCT ATGAAAAATC ATCAGAGGGA 
AAGACTTATG AAGATGACTC TCCTGAATGG TTTAAGGAAG ACAATGCTGT TCAGCCCAAC 
AGCACTTATA CATATGTATG GCACGCCACT GAGCGATCAG GGCCAGAAAA TCCTGGCTCT 
GCCTGTCGGG CTTGGGCCTA CTACTCAGCA GTGAACCCGG AAAAAGACAT CCACTCAGGC 
TTGATAGGAC CCCTTCTGAT CTGCCGAAAA GGAACACTTC ATAAGGAGAC CAACATGCCT 
GTGGACATGA GAGAATTTGT CCTACTTTTT ATGGTCTTTG ATGAAAAGAA GAGCTGGTAC 
TATGAAAAGA AGCCCATGAG GTCTTGGAGA CGCACATCCT CAGAAGTAAA AAACTCCCAT 
GAGTTTCATG CCATTAATGG GATGATCTAC AACTTGCCTG GCCTGAGAAT GTACGAGCAA 
GAGTGGGTGA GGTTGCACCT GCTGAATGTA GGCGGCTCCC AGGACATTCA TGTGGTTCAC 
TTTCATGGCC AGACCTTGCT GGAAAATGGC ACTCAACAGT ACCAGTTAGG GGTCTGGCCT 
CTTCTGCCTG GTTCATTTAA AACTCTTGAA ATGAAGGCTT CAAAACCTGG CTGGTGGCTC 
CTAGACACAG AAGTTGGAGA AAACCAGAGA GCAGGGATGC AAACGCTATT TCTCATCACA 
GACAGAGACT GTAAAATGCC AATGGGACTA AGCACTGGTC TCATAGCTGA TTCACAGATC 
AAGGCTTCTG AGTTTTGGGG TTATTGGGAA CCCAAATTAG CAAGACTAAA CAATGGTGGA 
TCATATAATG CTTGGATTGC AGAAAAACTT TCAGTAGAAT TTAACCCTAA ACCTTGGATC 
CAGGTAGACA TGCAAAGGGA AGTCCTGTTC ACGGGGATCC AGACCCAAGG TGCCAAACAC 
TACCTGAAGT CTTACTACAC CACCGAGTTC TTTGTGGCTT ACAGCTCTGA CCAGACAAAC 
TGGCGGATCT TCAAAGGGAA CAGCACTAGG AACGTGATGT ATTTTAATGG CAATTCAGAT 
GCCTCTACAA TAAAAGAGAA TCAGTTTGAC CCACCTGTTG TGGCTAGATA TATTAGAATC 
TCTCCAACTA AATCCTATAA CAAACCTGCC CTTCGATTGG AACTGCAAGG TTGCGAGGTT 
AACGGATGCT CCACACCTCT GGGTATGGAA AGTGGAAAGA TAGAAGACAA GCAAATCACA 
GCTTCCTCAT TTAAAAAATC TTGGTGGGGA GATTACTGGG AACCCTTCTG TGCCCGTCTT 
AATGCCCAGG GCCGTGTGAA TGCCTGGCAA GCCAAGGCAA ACAACAACAA TCAGTGGTTA 
CAAATTGATC TGCTCAAAAT CAAGAAGATA ACAGCAATTA TAACACAGGG TTGCAAGTCT 
CTGTCCTCTG AAATGTATGT GAAGAGCTAC ACCATCCAGT ACAGTGACCA GGGAGTTGAA 
TGGAAACCTT ACAGAGAGAA ATCCTCGTTG GTGGACAAGA TTTTCGAAGG AAATAATAAT 
ATCAAAGGAC ATGCAAAGAA CTTTTTCAAC CCACCAATCA TTTCCAGGTT TATACGCATC 
ATTCCTAAAA CATGGAATCA AAGCATTGCA CTTCGCCTGG AACTCTTTGG CTGTGATATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F5 ( XM_028488690.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_028344491.1 Physeter catodon coagulation factor V (F5), transcript variant X1, mRNA.

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CloneID	OPh328359
Clone ID Related Accession (Same CDS sequence)	XM_028488690.1
Accession Version	XM_028488690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	coagulation factor V isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041217.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAG CTTGCCCTTG CCTCTGGGTC CTCGTGGTCC TGGGCTCCAG CTGGGCAGGC 
TGGGGGAGCC TAGGGGCTGA AGCAGCGCGG CTAAGGCAGT TCTACGTGGC TGCTCAGAGC 
ATCAGCTGGA ACTATCTCCC CGAGTCCACA AACCTTAGTT CGAATACTTT TGCAACCTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACGATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAGGCAGA CAAACCCTTA AGCATCCATC CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCTTC TTACCCTGAC CACACATTCC CTGTGGAGAG GATGGATGAT 
TCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGAATA TCAGTGAGGA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACATAT ATCTATTACT CCTATGAAAA TCTGGTACAC 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACTGAG 
GATGGGATAC AGAAGATGTT TGACAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCCTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGTCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATTCAT TTCAATGGCC AGGTCCTGGA AGAGAACCAT 
CATAAGATGT CAGCCATCAC TCTCGTCAGT GCTACGTCCA CAACTGCAAA CATGACTGTG 
AGCCTAGAGG GAAAGTGGAT CATATCTTCG CTCATCCCAA AACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GGAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AGTACAGATC TCTGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGCATCCT TCACCAAACG CCTGGAGAAT CCCAATGTCA AAGAAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTTAAAATCA TGTTCAAAAA TATGGCCAGC 
CGCAGCTACA GCATTTACCC TCATGGTGTG ACCTTCTCTC CTTATGAAGA TGAAGTCAAC 
TCTTCCTTCG CCTCAGGCAG TAACACCATG ATCAAAGCAG TTCAACCAGG GGAAACCTAT 
ACTTATAAGT GGAACATCCT AGAGTCTGAT GAACCCATGG AAAATGATGC TCAATGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACA TTGCCTCTGG GCTGATAGGG 
CTAGTTCTAA TTTGTAAGAG CAGATCCTTG GATAAGCGAG GCATACAGAG GACAGCAGAC 
ATCGAGCAAC AGGCTGTGTT TGCTGTGTTT GATGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAGGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGCAC TATCAATGGC TATGTGCCTG AGAGCATACC CACACTAGGA 
TTCTGCTTTG ATGACACCGT CCAGTGGCAC TTCTGCAGTA TGGGAACCCA GAATGACATT 
TTGACCATTC ACTTCACTGG GCACTCATTC ATCTATGGAA AGAAACATGA GGACACCTTG 
ACCCTCTTCC CCATGCGTGG GGAATCTGTG ACCGTCACAA TGGATAACGT TGGAACTTGG 
ATGTTAACTA CCATGAATTC CAATCCAAGA AGCAAAAAAC TGTGGCTGAG ATTCAGGGAT 
GTTAAGTGTA TCCGGGAGTA TGATGATGAC TCATATGAGA TTATATACGA ACCTTCAGGA 
TCTACATCCA AAACTACAAG GAAAATGCGT GATTCTTCAG AAATCAAAGA TAAAACTAAT 
GATGCTGACT ATGATTACCA GAACACACTG GCTTTATTAT TAGGAATCAG GTCATTCAGA 
AATTCATCAT CAAATCAGGA GGAAGATGAG TTCAATCTTA CTGCCCTGGC TCTGGGGAAC 
AACTCTGAAT TCATTCCTCC AAGTACCAAC ACAACTGTTG GTTCAAATTC TTCTTCCCCA 
GGTAACATTA GCAGGCTTAT TGCCAATAAC TTTGCACAAC TTTTGAAAAT TCTTCCTCAC 
TCAGAAGCCA CCACAGCTGG TCCCCCACTG GGACACACCA GCTTAGATAA GAACTCAGTT 
CTCAACCCTC CCACAGCAGA GCATTCCAGC CCTTATTCTG AAGATCCTCT ATACTCAGAT 
GTCACAGGGA TAAGCCTGCT TCCATTTGCT ACAGAAGAAT TCAGAAATCA AGAACATGCT 
AAACGTAAAA GATTCAAGGC AGGAAGAGGC CGAGCAGCAA AGCAAAGGTT CTCCCAGATG 
GAATTCCTAG CACGTAAAAC TGGTAGACAT TTAAGCAAAG GCAACTCTTC TTCTTCCAGA 
ATAGGGCCCT GGGAGAACCT TCCCAGTGAT CTGTTACTCT TGCAACAAAA GGATCCATCT 
AAGATTCTGA ATGGGGAATG GCATTTGGTT TCTGAGAAAG GCAGTTATGA AATAATCCAA 
GATGCTGATG AAGACAAGGC TGTTAATAAG CTGCCAAACA GCCCCCAGAA TGACTCAAGG 
ACTTGGGGAG AAAACATCCC TTTTAAAAAC AAGCATGGAA AGCAGAGTGG CCACCCAACG 
TTTTTTGTAA TTAGACATAA ACCTCTACAA GGAAGACAAG ATGGAGGAAA TAGTAGATTG 
AAGAAAGGCC TTTTTTTAAT TAGGACACGA AGAAAGAAAA AGGAAGAGAA GCCTGCACAC 
CATGTTCCTC TATCTCCAAG GAGTTTTTAC CCTCTAAGAG TAGAGGCCAA CACCCCATCT 
TCAGATGGGA GACATAATCA TTCATTGTTA CTCCATACAT CCAATGAAAC ATCTCTTCCC 
ACAGACCTCA ATCAGACATT CCCCTCTATG AACCTTAGCC TCATAGCCTC ACTTCCTGAT 
GGTGATCAAA ACCCCCCAAA TGACACCATT CAGACAAGCT CCCCTCCAGA TCTTTATCAG 
ACAGTGAGCC CAGAGGAACA CTATCAAACA TTCCCTATTC AAGACTCTGA TCCAACGCAC 
TCCACTACAG TCCCCAGTCA CAGATATCCT CCTCCAGAGC CCAGCCAGAT ACCCGACTAT 
GACCTAAGAA ACAAGGCCTT TCCTACTGAT GTTAGTCAAA CGTTCCCTTC CTTGGAACTT 
GAAGTCTGGC AGACAACCAC CTCTCTAGAC CTCAGTCAGC CATCCCTCTA TCCAGATCTC 
AGCCAGACCA CCCTTTCTCC AGACCCTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT TGGCCAGGAG ACTCTCTCTC CAGACCTCAG CCAGACAACA 
CTTTCCCCAG ACCTTGGCCA GGAGACTCTC TCTCCAGACC TCAGCCAGAC AACACTTTCC 
CCAGTTCTTA GCCAGGAGAC TCTCTCTCCA GACCTCAGCC AGATGGCCCT TTCCCCACAC 
CCTGGCCAGG AGACGCTTTC TCCAGACTTC AGCCAGACAA CACTTTCCCC AGACCTTAGT 
CAGGAGACTC TCTCTCCAGA CCTCGGACAG ATAGCCCTTT CCCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACA TTTTCCCCAG ACTTCAGCCA GACTATCTCT 
CCAGACCTCA GACAGGAGAC TCTCTCTCCA GGCCTCAACC AGACAGCCCT TTCCCCAGAC 
CTTAGCCAGG AGACTCTCTC TCCAGACCTC AGCCAGACAA CATTTTCCCC AGACCTTAGC 
CAGGAGACTC TCTCTCCAGA CCTCAGCCCG ATGGCCCTTT CGCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACG CTTTCCCCAG ACCTCAGCCA GGCTCTCTCT 
CCAGACATCA GACAGATGGT CCTTTCCCCA GACCTCAGCC AGGAGATTCT CTCTCCAGAC 
CTCAGCCAGA CAACACCTTC CCCAGACCTT GGCCAGGAGA CTCTCTCTGC AGACCTCAGC 
CAGACAACAC TTTCCCCAGA CCTTGGCCAG GAGACTCTCT CTGCAGACCT CAGCCAGACA 
ACACTTTCCC CAGACCTCGG CCAGGAGACT CTCTCTCCAG ACCTCAGCCA GACAACACTT 
TCCCCAGACC TTGGCCAGGA GACTCTCTCT CCAGACCTCA GCCAGACAAC ACTTTCCCCA 
GACCTCAGCC AGGCTCTCTC TCCAGACATC AGCCAGATGG TCCTTTCCCC AGACCTCAGT 
CAGATGCCAC TTTCCCCAGA CCTCAACCAG GCAACTCTCT CTCCAGACAT CAGTCAGACA 
TCCCTTCCTC TTGATCTCAG GCAGACATCA CCTCCTCTAG ATCTCCATCA GACATCCCAC 
ACTTCTGAAT TTAGTCAGTC ATTGCCTCTC CCAGAATTTG GTCAGACTTT CCCTTATGCA 
GATCTTGGTC AGATGCCATC TCCTCCACCA CACTCTCCAC TAAATAACAC TTTTATACCA 
AGAGAATTTA ATCCACTGGT CATAGTAGGC CTCAGTAGAG ATGATGGAGA TTACATTGAA 
ATTATTCCAA GGCAGAAGGA AGAGAGCAGT GAAGAAGACT ATAGTGAATT TGATTTTGTG 
GCTTATGATG ACCCTTACCA AACTGATCTT AGGACAGACA TCAACTCCTC CAGAAATCCT 
GACAACATTG CAGCATGGTA CCTTCGCAGC AACAATGGAA ACAGAAAAAA TTATTACATT 
GCTGCTGAAG AACTATCCTG GGATTATTCA AAATTTGCAC AAAGTGAAGA CACTGATGAT 
GTTTCAAAGG ACACCATATA CAAGAAAGTA GTTTTTCGAA AGTACCTTGA TAGCACTTTT 
AAAAAGCTTG ATCCTCGGGG AGAGTATGAA GAGCATCTTG GCATACTTGG TCCTATCATT 
AGAGCTGAAG TGGATGATGT TATCCAAGTT CGTTTTAAAA ACTTAGCATC CAGACCATAT 
TCTCTTCATG CCCATGGGCT TTCCTATGAA AAATCATCAG AGGGAAAGAC TTATGAAGAT 
GACTCTCCTG AATGGTTTAA GGAAGACAAT GCTGTTCAGC CCAACAGCAC TTATACATAT 
GTATGGCACG CCACTGAGCG ATCAGGGCCA GAAAATCCTG GCTCTGCCTG TCGGGCTTGG 
GCCTACTACT CAGCAGTGAA CCCGGAAAAA GACATCCACT CAGGCTTGAT AGGACCCCTT 
CTGATCTGCC GAAAAGGAAC ACTTCATAAG GAGACCAACA TGCCTGTGGA CATGAGAGAA 
TTTGTCCTAC TTTTTATGGT CTTTGATGAA AAGAAGAGCT GGTACTATGA AAAGAAGCCC 
ATGAGGTCTT GGAGACGCAC ATCCTCAGAA GTAAAAAACT CCCATGAGTT TCATGCCATT 
AATGGGATGA TCTACAACTT GCCTGGCCTG AGAATGTACG AGCAAGAGTG GGTGAGGTTG 
CACCTGCTGA ATGTAGGCGG CTCCCAGGAC ATTCATGTGG TTCACTTTCA TGGCCAGACC 
TTGCTGGAAA ATGGCACTCA ACAGTACCAG TTAGGGGTCT GGCCTCTTCT GCCTGGTTCA 
TTTAAAACTC TTGAAATGAA GGCTTCAAAA CCTGGCTGGT GGCTCCTAGA CACAGAAGTT 
GGAGAAAACC AGAGAGCAGG GATGCAAACG CTATTTCTCA TCACAGACAG AGACTGTAAA 
ATGCCAATGG GACTAAGCAC TGGTCTCATA GCTGATTCAC AGATCAAGGC TTCTGAGTTT 
TGGGGTTATT GGGAACCCAA ATTAGCAAGA CTAAACAATG GTGGATCATA TAATGCTTGG 
ATTGCAGAAA AACTTTCAGT AGAATTTAAC CCTAAACCTT GGATCCAGGT AGACATGCAA 
AGGGAAGTCC TGTTCACGGG GATCCAGACC CAAGGTGCCA AACACTACCT GAAGTCTTAC 
TACACCACCG AGTTCTTTGT GGCTTACAGC TCTGACCAGA CAAACTGGCG GATCTTCAAA 
GGGAACAGCA CTAGGAACGT GATGTATTTT AATGGCAATT CAGATGCCTC TACAATAAAA 
GAGAATCAGT TTGACCCACC TGTTGTGGCT AGATATATTA GAATCTCTCC AACTAAATCC 
TATAACAAAC CTGCCCTTCG ATTGGAACTG CAAGGTTGCG AGGTTAACGG ATGCTCCACA 
CCTCTGGGTA TGGAAAGTGG AAAGATAGAA GACAAGCAAA TCACAGCTTC CTCATTTAAA 
AAATCTTGGT GGGGAGATTA CTGGGAACCC TTCTGTGCCC GTCTTAATGC CCAGGGCCGT 
GTGAATGCCT GGCAAGCCAA GGCAAACAAC AACAATCAGT GGTTACAAAT TGATCTGCTC 
AAAATCAAGA AGATAACAGC AATTATAACA CAGGGTTGCA AGTCTCTGTC CTCTGAAATG 
TATGTGAAGA GCTACACCAT CCAGTACAGT GACCAGGGAG TTGAATGGAA ACCTTACAGA 
GAGAAATCCT CGTTGGTGGA CAAGATTTTC GAAGGAAATA ATAATATCAA AGGACATGCA 
AAGAACTTTT TCAACCCACC AATCATTTCC AGGTTTATAC GCATCATTCC TAAAACATGG 
AATCAAAGCA TTGCACTTCG CCTGGAACTC TTTGGCTGTG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028344491.1
CDS	27..6974
Translation	MFPACPCLWVLVVLGSSWAGWGSLGAEAARLRQFYVAAQSISWNYLPESTNLSSNTFATSFKKIVYREYEAYFQKEKPRSRTSGLLGPTLYAEVGDIMKVHFKNKADKPLSIHPQGIKYSKFSEGASYPDHTFPVERMDDSVAPGQEYTYEWNISEDSGPTHDDPPCLTYIYYSYENLVHDFNSGLIGPLLICKKGTLTEDGIQKMFDKQHVLMFAVFDESKSWNQTSSLMYTVNGYVNGTMPDITVCVHDHISWHLIGMSSGPELFSIHFNGQVLEENHHKMSAITLVSATSTTANMTVSLEGKWIISSLIPKHFQAGMQAYIDIKNCAKKTRNPKKLTRDQRRHIKRWEYFIAAEEVIWDYAPIIPANMDKKYRSLHLDNFSNQIGKHYKKVVYKQYQDASFTKRLENPNVKEDGILGPIIRAQVRDTLKIMFKNMASRSYSIYPHGVTFSPYEDEVNSSFASGSNTMIKAVQPGETYTYKWNILESDEPMENDAQCLTRPYYSNVDITRDIASGLIGLVLICKSRSLDKRGIQRTADIEQQAVFAVFDENKSWYIEDNIYKFCENPEKVKRDDPKFYESNIMSTINGYVPESIPTLGFCFDDTVQWHFCSMGTQNDILTIHFTGHSFIYGKKHEDTLTLFPMRGESVTVTMDNVGTWMLTTMNSNPRSKKLWLRFRDVKCIREYDDDSYEIIYEPSGSTSKTTRKMRDSSEIKDKTNDADYDYQNTLALLLGIRSFRNSSSNQEEDEFNLTALALGNNSEFIPPSTNTTVGSNSSSPGNISRLIANNFAQLLKILPHSEATTAGPPLGHTSLDKNSVLNPPTAEHSSPYSEDPLYSDVTGISLLPFATEEFRNQEHAKRKRFKAGRGRAAKQRFSQMEFLARKTGRHLSKGNSSSSRIGPWENLPSDLLLLQQKDPSKILNGEWHLVSEKGSYEIIQDADEDKAVNKLPNSPQNDSRTWGENIPFKNKHGKQSGHPTFFVIRHKPLQGRQDGGNSRLKKGLFLIRTRRKKKEEKPAHHVPLSPRSFYPLRVEANTPSSDGRHNHSLLLHTSNETSLPTDLNQTFPSMNLSLIASLPDGDQNPPNDTIQTSSPPDLYQTVSPEEHYQTFPIQDSDPTHSTTVPSHRYPPPEPSQIPDYDLRNKAFPTDVSQTFPSLELEVWQTTTSLDLSQPSLYPDLSQTTLSPDPGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPVLSQETLSPDLSQMALSPHPGQETLSPDFSQTTLSPDLSQETLSPDLGQIALSPDLGQETLSPDLSQTTFSPDFSQTISPDLRQETLSPGLNQTALSPDLSQETLSPDLSQTTFSPDLSQETLSPDLSPMALSPDLGQETLSPDLSQTTLSPDLSQALSPDIRQMVLSPDLSQEILSPDLSQTTPSPDLGQETLSADLSQTTLSPDLGQETLSADLSQTTLSPDLGQETLSPDLSQTTLSPDLGQETLSPDLSQTTLSPDLSQALSPDISQMVLSPDLSQMPLSPDLNQATLSPDISQTSLPLDLRQTSPPLDLHQTSHTSEFSQSLPLPEFGQTFPYADLGQMPSPPPHSPLNNTFIPREFNPLVIVGLSRDDGDYIEIIPRQKEESSEEDYSEFDFVAYDDPYQTDLRTDINSSRNPDNIAAWYLRSNNGNRKNYYIAAEELSWDYSKFAQSEDTDDVSKDTIYKKVVFRKYLDSTFKKLDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKTYEDDSPEWFKEDNAVQPNSTYTYVWHATERSGPENPGSACRAWAYYSAVNPEKDIHSGLIGPLLICRKGTLHKETNMPVDMREFVLLFMVFDEKKSWYYEKKPMRSWRRTSSEVKNSHEFHAINGMIYNLPGLRMYEQEWVRLHLLNVGGSQDIHVVHFHGQTLLENGTQQYQLGVWPLLPGSFKTLEMKASKPGWWLLDTEVGENQRAGMQTLFLITDRDCKMPMGLSTGLIADSQIKASEFWGYWEPKLARLNNGGSYNAWIAEKLSVEFNPKPWIQVDMQREVLFTGIQTQGAKHYLKSYYTTEFFVAYSSDQTNWRIFKGNSTRNVMYFNGNSDASTIKENQFDPPVVARYIRISPTKSYNKPALRLELQGCEVNGCSTPLGMESGKIEDKQITASSFKKSWWGDYWEPFCARLNAQGRVNAWQAKANNNNQWLQIDLLKIKKITAIITQGCKSLSSEMYVKSYTIQYSDQGVEWKPYREKSSLVDKIFEGNNNIKGHAKNFFNPPIISRFIRIIPKTWNQSIALRLELFGCDI

Target ORF information:

RefSeq Version	XM_028488690.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon coagulation factor V (F5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028488690.1

ORF Insert Sequence:

ATGTTCCCAG CTTGCCCTTG CCTCTGGGTC CTCGTGGTCC TGGGCTCCAG CTGGGCAGGC 
TGGGGGAGCC TAGGGGCTGA AGCAGCGCGG CTAAGGCAGT TCTACGTGGC TGCTCAGAGC 
ATCAGCTGGA ACTATCTCCC CGAGTCCACA AACCTTAGTT CGAATACTTT TGCAACCTCC 
TTTAAGAAAA TTGTCTACAG AGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACGATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTA TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAGGCAGA CAAACCCTTA AGCATCCATC CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCTTC TTACCCTGAC CACACATTCC CTGTGGAGAG GATGGATGAT 
TCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGAATA TCAGTGAGGA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACATAT ATCTATTACT CCTATGAAAA TCTGGTACAC 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACTGAG 
GATGGGATAC AGAAGATGTT TGACAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCCTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACAATGCCAG ATATAACAGT TTGTGTCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATTCAT TTCAATGGCC AGGTCCTGGA AGAGAACCAT 
CATAAGATGT CAGCCATCAC TCTCGTCAGT GCTACGTCCA CAACTGCAAA CATGACTGTG 
AGCCTAGAGG GAAAGTGGAT CATATCTTCG CTCATCCCAA AACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GGAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AGTACAGATC TCTGCATTTG 
GATAATTTCT CAAACCAAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGCATCCT TCACCAAACG CCTGGAGAAT CCCAATGTCA AAGAAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTTAAAATCA TGTTCAAAAA TATGGCCAGC 
CGCAGCTACA GCATTTACCC TCATGGTGTG ACCTTCTCTC CTTATGAAGA TGAAGTCAAC 
TCTTCCTTCG CCTCAGGCAG TAACACCATG ATCAAAGCAG TTCAACCAGG GGAAACCTAT 
ACTTATAAGT GGAACATCCT AGAGTCTGAT GAACCCATGG AAAATGATGC TCAATGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACA TTGCCTCTGG GCTGATAGGG 
CTAGTTCTAA TTTGTAAGAG CAGATCCTTG GATAAGCGAG GCATACAGAG GACAGCAGAC 
ATCGAGCAAC AGGCTGTGTT TGCTGTGTTT GATGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAGGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGCAC TATCAATGGC TATGTGCCTG AGAGCATACC CACACTAGGA 
TTCTGCTTTG ATGACACCGT CCAGTGGCAC TTCTGCAGTA TGGGAACCCA GAATGACATT 
TTGACCATTC ACTTCACTGG GCACTCATTC ATCTATGGAA AGAAACATGA GGACACCTTG 
ACCCTCTTCC CCATGCGTGG GGAATCTGTG ACCGTCACAA TGGATAACGT TGGAACTTGG 
ATGTTAACTA CCATGAATTC CAATCCAAGA AGCAAAAAAC TGTGGCTGAG ATTCAGGGAT 
GTTAAGTGTA TCCGGGAGTA TGATGATGAC TCATATGAGA TTATATACGA ACCTTCAGGA 
TCTACATCCA AAACTACAAG GAAAATGCGT GATTCTTCAG AAATCAAAGA TAAAACTAAT 
GATGCTGACT ATGATTACCA GAACACACTG GCTTTATTAT TAGGAATCAG GTCATTCAGA 
AATTCATCAT CAAATCAGGA GGAAGATGAG TTCAATCTTA CTGCCCTGGC TCTGGGGAAC 
AACTCTGAAT TCATTCCTCC AAGTACCAAC ACAACTGTTG GTTCAAATTC TTCTTCCCCA 
GGTAACATTA GCAGGCTTAT TGCCAATAAC TTTGCACAAC TTTTGAAAAT TCTTCCTCAC 
TCAGAAGCCA CCACAGCTGG TCCCCCACTG GGACACACCA GCTTAGATAA GAACTCAGTT 
CTCAACCCTC CCACAGCAGA GCATTCCAGC CCTTATTCTG AAGATCCTCT ATACTCAGAT 
GTCACAGGGA TAAGCCTGCT TCCATTTGCT ACAGAAGAAT TCAGAAATCA AGAACATGCT 
AAACGTAAAA GATTCAAGGC AGGAAGAGGC CGAGCAGCAA AGCAAAGGTT CTCCCAGATG 
GAATTCCTAG CACGTAAAAC TGGTAGACAT TTAAGCAAAG GCAACTCTTC TTCTTCCAGA 
ATAGGGCCCT GGGAGAACCT TCCCAGTGAT CTGTTACTCT TGCAACAAAA GGATCCATCT 
AAGATTCTGA ATGGGGAATG GCATTTGGTT TCTGAGAAAG GCAGTTATGA AATAATCCAA 
GATGCTGATG AAGACAAGGC TGTTAATAAG CTGCCAAACA GCCCCCAGAA TGACTCAAGG 
ACTTGGGGAG AAAACATCCC TTTTAAAAAC AAGCATGGAA AGCAGAGTGG CCACCCAACG 
TTTTTTGTAA TTAGACATAA ACCTCTACAA GGAAGACAAG ATGGAGGAAA TAGTAGATTG 
AAGAAAGGCC TTTTTTTAAT TAGGACACGA AGAAAGAAAA AGGAAGAGAA GCCTGCACAC 
CATGTTCCTC TATCTCCAAG GAGTTTTTAC CCTCTAAGAG TAGAGGCCAA CACCCCATCT 
TCAGATGGGA GACATAATCA TTCATTGTTA CTCCATACAT CCAATGAAAC ATCTCTTCCC 
ACAGACCTCA ATCAGACATT CCCCTCTATG AACCTTAGCC TCATAGCCTC ACTTCCTGAT 
GGTGATCAAA ACCCCCCAAA TGACACCATT CAGACAAGCT CCCCTCCAGA TCTTTATCAG 
ACAGTGAGCC CAGAGGAACA CTATCAAACA TTCCCTATTC AAGACTCTGA TCCAACGCAC 
TCCACTACAG TCCCCAGTCA CAGATATCCT CCTCCAGAGC CCAGCCAGAT ACCCGACTAT 
GACCTAAGAA ACAAGGCCTT TCCTACTGAT GTTAGTCAAA CGTTCCCTTC CTTGGAACTT 
GAAGTCTGGC AGACAACCAC CTCTCTAGAC CTCAGTCAGC CATCCCTCTA TCCAGATCTC 
AGCCAGACCA CCCTTTCTCC AGACCCTGGC CAGGAGACTC TCTCTCCAGA CCTCAGCCAG 
ACAACACTTT CCCCAGACCT TGGCCAGGAG ACTCTCTCTC CAGACCTCAG CCAGACAACA 
CTTTCCCCAG ACCTTGGCCA GGAGACTCTC TCTCCAGACC TCAGCCAGAC AACACTTTCC 
CCAGTTCTTA GCCAGGAGAC TCTCTCTCCA GACCTCAGCC AGATGGCCCT TTCCCCACAC 
CCTGGCCAGG AGACGCTTTC TCCAGACTTC AGCCAGACAA CACTTTCCCC AGACCTTAGT 
CAGGAGACTC TCTCTCCAGA CCTCGGACAG ATAGCCCTTT CCCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACA TTTTCCCCAG ACTTCAGCCA GACTATCTCT 
CCAGACCTCA GACAGGAGAC TCTCTCTCCA GGCCTCAACC AGACAGCCCT TTCCCCAGAC 
CTTAGCCAGG AGACTCTCTC TCCAGACCTC AGCCAGACAA CATTTTCCCC AGACCTTAGC 
CAGGAGACTC TCTCTCCAGA CCTCAGCCCG ATGGCCCTTT CGCCAGACCT TGGCCAGGAG 
ACTCTCTCTC CAGACCTCAG CCAGACAACG CTTTCCCCAG ACCTCAGCCA GGCTCTCTCT 
CCAGACATCA GACAGATGGT CCTTTCCCCA GACCTCAGCC AGGAGATTCT CTCTCCAGAC 
CTCAGCCAGA CAACACCTTC CCCAGACCTT GGCCAGGAGA CTCTCTCTGC AGACCTCAGC 
CAGACAACAC TTTCCCCAGA CCTTGGCCAG GAGACTCTCT CTGCAGACCT CAGCCAGACA 
ACACTTTCCC CAGACCTCGG CCAGGAGACT CTCTCTCCAG ACCTCAGCCA GACAACACTT 
TCCCCAGACC TTGGCCAGGA GACTCTCTCT CCAGACCTCA GCCAGACAAC ACTTTCCCCA 
GACCTCAGCC AGGCTCTCTC TCCAGACATC AGCCAGATGG TCCTTTCCCC AGACCTCAGT 
CAGATGCCAC TTTCCCCAGA CCTCAACCAG GCAACTCTCT CTCCAGACAT CAGTCAGACA 
TCCCTTCCTC TTGATCTCAG GCAGACATCA CCTCCTCTAG ATCTCCATCA GACATCCCAC 
ACTTCTGAAT TTAGTCAGTC ATTGCCTCTC CCAGAATTTG GTCAGACTTT CCCTTATGCA 
GATCTTGGTC AGATGCCATC TCCTCCACCA CACTCTCCAC TAAATAACAC TTTTATACCA 
AGAGAATTTA ATCCACTGGT CATAGTAGGC CTCAGTAGAG ATGATGGAGA TTACATTGAA 
ATTATTCCAA GGCAGAAGGA AGAGAGCAGT GAAGAAGACT ATAGTGAATT TGATTTTGTG 
GCTTATGATG ACCCTTACCA AACTGATCTT AGGACAGACA TCAACTCCTC CAGAAATCCT 
GACAACATTG CAGCATGGTA CCTTCGCAGC AACAATGGAA ACAGAAAAAA TTATTACATT 
GCTGCTGAAG AACTATCCTG GGATTATTCA AAATTTGCAC AAAGTGAAGA CACTGATGAT 
GTTTCAAAGG ACACCATATA CAAGAAAGTA GTTTTTCGAA AGTACCTTGA TAGCACTTTT 
AAAAAGCTTG ATCCTCGGGG AGAGTATGAA GAGCATCTTG GCATACTTGG TCCTATCATT 
AGAGCTGAAG TGGATGATGT TATCCAAGTT CGTTTTAAAA ACTTAGCATC CAGACCATAT 
TCTCTTCATG CCCATGGGCT TTCCTATGAA AAATCATCAG AGGGAAAGAC TTATGAAGAT 
GACTCTCCTG AATGGTTTAA GGAAGACAAT GCTGTTCAGC CCAACAGCAC TTATACATAT 
GTATGGCACG CCACTGAGCG ATCAGGGCCA GAAAATCCTG GCTCTGCCTG TCGGGCTTGG 
GCCTACTACT CAGCAGTGAA CCCGGAAAAA GACATCCACT CAGGCTTGAT AGGACCCCTT 
CTGATCTGCC GAAAAGGAAC ACTTCATAAG GAGACCAACA TGCCTGTGGA CATGAGAGAA 
TTTGTCCTAC TTTTTATGGT CTTTGATGAA AAGAAGAGCT GGTACTATGA AAAGAAGCCC 
ATGAGGTCTT GGAGACGCAC ATCCTCAGAA GTAAAAAACT CCCATGAGTT TCATGCCATT 
AATGGGATGA TCTACAACTT GCCTGGCCTG AGAATGTACG AGCAAGAGTG GGTGAGGTTG 
CACCTGCTGA ATGTAGGCGG CTCCCAGGAC ATTCATGTGG TTCACTTTCA TGGCCAGACC 
TTGCTGGAAA ATGGCACTCA ACAGTACCAG TTAGGGGTCT GGCCTCTTCT GCCTGGTTCA 
TTTAAAACTC TTGAAATGAA GGCTTCAAAA CCTGGCTGGT GGCTCCTAGA CACAGAAGTT 
GGAGAAAACC AGAGAGCAGG GATGCAAACG CTATTTCTCA TCACAGACAG AGACTGTAAA 
ATGCCAATGG GACTAAGCAC TGGTCTCATA GCTGATTCAC AGATCAAGGC TTCTGAGTTT 
TGGGGTTATT GGGAACCCAA ATTAGCAAGA CTAAACAATG GTGGATCATA TAATGCTTGG 
ATTGCAGAAA AACTTTCAGT AGAATTTAAC CCTAAACCTT GGATCCAGGT AGACATGCAA 
AGGGAAGTCC TGTTCACGGG GATCCAGACC CAAGGTGCCA AACACTACCT GAAGTCTTAC 
TACACCACCG AGTTCTTTGT GGCTTACAGC TCTGACCAGA CAAACTGGCG GATCTTCAAA 
GGGAACAGCA CTAGGAACGT GATGTATTTT AATGGCAATT CAGATGCCTC TACAATAAAA 
GAGAATCAGT TTGACCCACC TGTTGTGGCT AGATATATTA GAATCTCTCC AACTAAATCC 
TATAACAAAC CTGCCCTTCG ATTGGAACTG CAAGGTTGCG AGGTTAACGG ATGCTCCACA 
CCTCTGGGTA TGGAAAGTGG AAAGATAGAA GACAAGCAAA TCACAGCTTC CTCATTTAAA 
AAATCTTGGT GGGGAGATTA CTGGGAACCC TTCTGTGCCC GTCTTAATGC CCAGGGCCGT 
GTGAATGCCT GGCAAGCCAA GGCAAACAAC AACAATCAGT GGTTACAAAT TGATCTGCTC 
AAAATCAAGA AGATAACAGC AATTATAACA CAGGGTTGCA AGTCTCTGTC CTCTGAAATG 
TATGTGAAGA GCTACACCAT CCAGTACAGT GACCAGGGAG TTGAATGGAA ACCTTACAGA 
GAGAAATCCT CGTTGGTGGA CAAGATTTTC GAAGGAAATA ATAATATCAA AGGACATGCA 
AAGAACTTTT TCAACCCACC AATCATTTCC AGGTTTATAC GCATCATTCC TAAAACATGG 
AATCAAAGCA TTGCACTTCG CCTGGAACTC TTTGGCTGTG ATATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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F5 cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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