DDX60 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX60
Entrez Gene ID	102974719
Full Name	DExD/H-box helicase 60
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024130901.1	XP_023986669.1	probable ATP-dependent RNA helicase DDX60
XM_028494281.1	XP_028350082.1	probable ATP-dependent RNA helicase DDX60 isoform X1
XM_028494282.1	XP_028350083.1	probable ATP-dependent RNA helicase DDX60 isoform X2
XM_007116318.1	XP_007116380.1	probable ATP-dependent RNA helicase DDX60

Pathways from BioSystems
Gene Ontology

The following DDX60 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX60 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh129565	XM_007116318.1 Latest version!	Physeter catodon DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 (DDX60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh299792	XM_024130901.1 Latest version!	Physeter catodon DExD/H-box helicase 60 (LOC102974719), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh331883	XM_028494281.1 Latest version!	Physeter catodon DExD/H-box helicase 60 (LOC102974719), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPh331884	XM_028494282.1 Latest version!	Physeter catodon DExD/H-box helicase 60 (LOC102974719), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX60 ( XM_007116318.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007116380.1 Physeter catodon DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 (DDX60), mRNA.

Price & Availability↑

CloneID	OPh129565
Clone ID Related Accession (Same CDS sequence)	XM_007116318.1
Accession Version	XM_007116318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	probable ATP-dependent RNA helicase DDX60
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006715215.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTAG AAAAGTACCT GATGCTTTTA GAGAAAATGT CACAGTTGAT TTTGGATCAA 
ATGGCAAAAG CTGATTATGA CAGTCTGTTC AATGATTTTG TTGAGTCTGA GTTTTTTCTG 
ATTGATGGAG ACTCACTGCT GATGACGTAC GTCTGTGAGA AATCCTTGCG GCCTGAACAG 
AGCCTGCACT TCTTCTACCT GGTTGAACAC TACCTTGTGG ACCTAATTGG TAAAGGAGGA 
CAGTTTGCCA TCGTTTTCTT CGAGGATGCT GAGCATGCAT ACAACAACAT CCCTGAATTT 
CTTCCTCTGA GAATGGCATT AATTCTCCAC CTTCAGCATA ATACCAACAT TGATGTTCGA 
ACAGACTTTT CTGGGTGCTT CTCAGAAGAG TGGAAAACTT TCTTGGAAGA CCGTTACCCA 
TATTTTCTGA TCGTTTCCGA TGAAAGCTTG AGCCATCTAC AAGTATACCT TTTCAACTTT 
TTAATCATTC AGTCCTGGGC AATGAAGGTC AATGCCGTGC TCTTCTCGGG CCAAGCATCC 
GATGTCCTTC GACTTTATGC GTACTTTATG CCGAGCAAGT ACGAAAACCA GATGTTTTTC 
CAGAAGAATG AAAAGAACAT TGAGAATGCT TATAAAACCC TGATTAAAGA ATTGGAGGAA 
TGTAGAGTTT TAGCATTAGG ACATCTTTTT GGAAATTTAA AATGGAAAAA TATGCTGGAA 
GAGGCATGTG AAACTATTTC TCTGCTTAAG CAACTGTGGC CGGAAGGATC TGACATTCGG 
CAAGTTCTTT GTGTTACTTC ATGCTCCTTG TCTTTGAGTT TATACCATCA CTTTTTAGAA 
AACAGATCGA AGACCACCGA TGCTCAGAAA GCTAACATCC AGCAGATGGA GAGCGACTGT 
CCCACCCTGG AGGGGGTGAC AGACTGGTGT AGACTGCACT GTCTCAGCGT GGCCTTTCTC 
CTCCACTTAC CTCTTTCCCA GAGAGCATGT ACCAGATTCA TCACTTCCCA CTGGATTAAG 
GACATTCAGA CTTTCTTAAC AATGAAAAAG TGGTGTGAAT ACTTCATCCT AAGTAATATA 
GACGCTTTTG AATTTTGGAA TCTGAATTTA ACTCACCTTT CCGACTTAAG TGATGAGCTT 
TTATTGAAGA ATATAGCATT TTACTATGAA AATGAAAGCG TTCAAGGGCT ACATTTGAAT 
TTGGGAAATG TCATTATGAA AGATTATGAA CATCTCTGGG ATACTGTATC AAAGTTGGTC 
ACAAAGTTTG ATGTTGGAAA GCCATTTCCT CTGAGAACAA AACAATTTCC TTTTCTTGAA 
AAGGATCCAT CACCAATCAG AGATACCTCC AAGGAAAAGA TGGCTAATTT GGGTTTTATT 
CCAGTGTCAT GTCAGGTGGT TGATAAGTTT GCTGGGGACA TTTTGAAAGA TTTGCCTTTC 
CTAACGAGCG ATGATCCTAT TGTAACTTCA CTGGTCAAGC AGAAAGAATT TGATGAGCTT 
GTGCACTGGC ATTCTCACAG ACCCCTCAGT GATGATTATG ACAGGACAAA GGGCCACTTT 
GACGAAAAAT CTAGAGATCC TCGTCATCTT AGATCTGTGC AAATGTATCA TCGTTTTCAG 
CGATTTTATG GGAAGTCATT AGAATCAGTC TCTTCAAAAT TTGTTGTACC TCAAGCTATC 
AAGCCAAAGA AGAATTTCTG TGAACCCAAG AGCAAAAAGA TACAGGAGAC CAAGGCTGAA 
AAAATTACTA GAGAGAATAA GGAGAGGCTA TTTGCCAAGG AAGAACAAAA AGAAGAGAAA 
AAATGGAATG TTATGTCCTT TTCTATTGAG GAGGAAATGA AAGAGAATTT AAACTCTGGA 
ATAAAGAACC TAGAAGAGTT TTTGAAATCA TGTAAAAGTA GCTCAGTGAA ATTGCGAGTT 
GAAATGGTGG GGATTACTGC CTGCTTGAGA GCGTGGAAAG AACATTGCCG TGGTGAAGGT 
GAAACCACAA AAGATTTAAG TATAGCTGTT CAAATGATGA AAAGGATCTA CTCCGTGACG 
GAAAAATACC CAGAACTTTT GCAAGAAGCA GATTGGCAAC TCATAGCCAG ATGCCTTAGG 
TATTTAGGAT TTGAGGAGTT GGCAAGTTCT TTGCATCCAA CCCAGGTCAC AGGAAATGAC 
GTAAAGGAGA AGAAAAAGAA CAAATACTCA GTTGGCATGG GGCCAGCTCG GTTTCAGCTG 
CAGTACATGG GCCACTATTT GGTACGAGAT GAGAGGAAAG ATCCAGACCC CAGAGTCCAG 
GACTTTATTC CTGACACCTG GCAGCGAGAG CTCCTGGACG TTGTGGACAA GAACGAGTCT 
GCGGTCATAG TGGCCCCCAC GTCCTCAGGG AAGACCTACG CCTCCTACTA CTGCATGGAG 
AAGGTGCTGC GGGAGGGTGA CGACGGGGTG GTCGTGTACG TGGCCCCCAC CAAGGCCCTT 
GTCAATCAGG TGGCAGCAAC TGTTGAGAAT CGTTTTAACA AAAATCTGCC AGACGGTGAA 
ACTCTGTGTG GTGTTTTTAC ACGGGACTAT CGTCATGAGG CTCTAAACTC TCAGGTACTT 
ATCACAGTGC CTGCCTGCTT TGAAATTCTG CTGCTAGCTC CGCATCGCCA GGCGTGGGTG 
AAAAGGATCA GATACGTTAT ATTTGATGAG GTCCACTGTC TTGGTGGAGA AATTGGAGCA 
GAAGTCTGGG AGCATCTCCT TGTCATGATC CGCTGTCCCT TCCTGGCCCT TTCTGCTACC 
ATCAGCAACC CGGAGCATCT CACCGAGTGG CTACGTTCCA TAAAACAGTA CTGGAAACAA 
ACAGACAGTA CGATGGGAAA AAATATTGCT TCTTCAAGAA ATGCCGGCTG CCGTGCCAGT 
TCTCGCCAAG ACTACTTACT GATGAAGCAG TCGTATAAAG TGAGACTTGT GCTCTATGCA 
GAGAGATACA ATGATTTAGA GAAGCACATA TGTTCAGTAA ACAATCATGA CATTTCCTTT 
GACCATTTTC ACCCGTGCGC TGCGCTGACC ACGGACCATA TTGAAAAGTT TGGATTCCCT 
TCTGATCTTA CCCTTTCACC GCGAGAAATG ATCCAGCTTT ATGATGCCAT GTTTGAATTC 
TGGGAAAGTT GGCCCAGGGC TCAGGAGTTG TGTCCGGAGG AATTCGTTCA TTTTAAGAAT 
AAAATAGTCA TTCAAAAGAT GGATGCTAGA AAGTATGAAG AGAGCTTAAA GGCAGAACTG 
ACAGGCTGGG TGAAGAGCGG TCACGTCAAG GAGGCCAGAA TGGTCCTGAG GAATCTTCGT 
CCTCCTTCGG CTGTTAGCTC AGAAAACATG GCAGTTATGT TTCCACGTCT CGTTGAAAAG 
CTAAGGCAAA TGGAGAAGTT ACCTGCACTG TTTTTTTTAT TTAAATTAGG GGCCGTAGAG 
AGAAGCGCTG AAAGTGTGAA AACGTTCCTA GAGATAAAGC AACAGAACAG AAGGCCCCCC 
AAGGCTGATA AGGAGGCCTA TGACCTGGAG AACAAACTGC GAAAAGTTAG AAAATCCCTC 
GAGGCACAGA AGACAGTAGA TAAAAGAAGC CAGAAAAATC CCAGAAAAAT GGATCAAAGC 
CTAATCCATG AAGCTGAACA TGATAGTCTC CTCAGGGTTC TTGAGAAGAA CTTGGAGATC 
CCTCAGGACT GCACATATGC TGATCAGAAG GCCATAGATG CTGAGACTTT GCAGAGAGTG 
TATGACCGAG TGAAATTCTC AAGGAAAGGC GCCAAACTGA AGGCTTTGGC AGCAAGGGGC 
ATCGGATATC ATCACAGCTC TTTGAGCGCC AAAGAAAAGC AGTTAGTTGA GATTCTTTTC 
AGGAAGGGAT TTATTAGGGT GGTGACCGCT ACAGGGACAC TTGCTTTAGG AATCAACATG 
CCTTGTAAAT CTGTGGTTTT TGCTCAAAAC TCAGTCTATC TGGATGCTTT AAACTACAGA 
CAGATGTCTG GACGGGCCGG ACGACGAGGT CAAGACCTGG GGGGAGATGT GTATTTCTTT 
GATATTCCAC TCCCTAAAAT CGAAAAGCTC ATCAAATCCA ACGTCCCTGA ACTGAGAGGG 
CAGTTCCCTC TCAGCATATC CCTGATCCTG AGACTCATGT GCCTGGCTTC CAAGGGAGAT 
GACCCCGAGG ACGCTAGGGC AAAGGTGCTG TCCGTGCTCA AGCACTCACT GCTCTCCTTC 
AAGCAGCCCC GGGTGCTGGA GATGCTGAAA CTGTACTTTC TGTTCTCCCT GCAATTCCTG 
GTCAAGGAGG GCTATTTAGA TCAAGAAGGT AATCCTACAG GCTTTGCTGG ACTTGTGTCA 
CATTTGCATT ATCACGAGCC TTCTAATCTT GTTTTCGTCG GTTTTCTTGT AAGAGGCCTT 
TTCCATAATC TCTGTCAGCC GACCAGCAAA GGCTCAAAAC ATTTTTCTCA AGATGTTATG 
GAAAAGCTAG TGTTAGTATT GGCAAATCTG TTTGGAAGAA AATATCTTCC AGCACAGTTC 
CAAGATGCTA CGCTCAAGTT TTATCAATCC AAGGTGTTTC TGGAGGATCT CCCTGAGGAC 
TTCAAGGCTG CTCTAGTTGA GTATAACACA AATGTGACAA AGGACTTTGC CTCTTTCCTA 
CTGATTGTTT CCAGACTGGC TGATATGAAA CAAGAACATC AGCTCCCATT GTCAAAAATA 
GATTTCTCAG GTGAAGAGTG TGAAGACTCC CAACTTGTGT CTCACTTGAT GAGCTGCAAG 
GAAGGAAGAG TAGCCATTTC TCCATTTGCC TGTCTCTCTG GGAACTCTGA TGGAAATTTG 
CTTCATCTAG AAACTTCTGA CCATGTCACT CTGCGCACCA TTGGCATTGA TAGCACTCGG 
GCTCCTGTGC TGTGGCCACA GGGATTTGAT CACCAAGGAA GAAGAATGCA GCTCAATGCT 
TATGCACTCG ATTTCTATAG ACACGGTTCC TTGACAGGAT TGGTCCAGGA CAACAGGATG 
AATGAAGGAG CTGCTTATCA GTTGTTGAAA GATTTTGGGC TCACCATTCA ATCTATCAGT 
GTTTCTTTGC GTGAACTATG TGAAAATGAA GAGGACAACG TGGTCTTAGC ATTTGAACAA 
CTGAGTGAAA CCTTTTTTGA AAAATTTAAC AAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007116380.1
CDS	84..5222
Translation	MGLEKYLMLLEKMSQLILDQMAKADYDSLFNDFVESEFFLIDGDSLLMTYVCEKSLRPEQSLHFFYLVEHYLVDLIGKGGQFAIVFFEDAEHAYNNIPEFLPLRMALILHLQHNTNIDVRTDFSGCFSEEWKTFLEDRYPYFLIVSDESLSHLQVYLFNFLIIQSWAMKVNAVLFSGQASDVLRLYAYFMPSKYENQMFFQKNEKNIENAYKTLIKELEECRVLALGHLFGNLKWKNMLEEACETISLLKQLWPEGSDIRQVLCVTSCSLSLSLYHHFLENRSKTTDAQKANIQQMESDCPTLEGVTDWCRLHCLSVAFLLHLPLSQRACTRFITSHWIKDIQTFLTMKKWCEYFILSNIDAFEFWNLNLTHLSDLSDELLLKNIAFYYENESVQGLHLNLGNVIMKDYEHLWDTVSKLVTKFDVGKPFPLRTKQFPFLEKDPSPIRDTSKEKMANLGFIPVSCQVVDKFAGDILKDLPFLTSDDPIVTSLVKQKEFDELVHWHSHRPLSDDYDRTKGHFDEKSRDPRHLRSVQMYHRFQRFYGKSLESVSSKFVVPQAIKPKKNFCEPKSKKIQETKAEKITRENKERLFAKEEQKEEKKWNVMSFSIEEEMKENLNSGIKNLEEFLKSCKSSSVKLRVEMVGITACLRAWKEHCRGEGETTKDLSIAVQMMKRIYSVTEKYPELLQEADWQLIARCLRYLGFEELASSLHPTQVTGNDVKEKKKNKYSVGMGPARFQLQYMGHYLVRDERKDPDPRVQDFIPDTWQRELLDVVDKNESAVIVAPTSSGKTYASYYCMEKVLREGDDGVVVYVAPTKALVNQVAATVENRFNKNLPDGETLCGVFTRDYRHEALNSQVLITVPACFEILLLAPHRQAWVKRIRYVIFDEVHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPEHLTEWLRSIKQYWKQTDSTMGKNIASSRNAGCRASSRQDYLLMKQSYKVRLVLYAERYNDLEKHICSVNNHDISFDHFHPCAALTTDHIEKFGFPSDLTLSPREMIQLYDAMFEFWESWPRAQELCPEEFVHFKNKIVIQKMDARKYEESLKAELTGWVKSGHVKEARMVLRNLRPPSAVSSENMAVMFPRLVEKLRQMEKLPALFFLFKLGAVERSAESVKTFLEIKQQNRRPPKADKEAYDLENKLRKVRKSLEAQKTVDKRSQKNPRKMDQSLIHEAEHDSLLRVLEKNLEIPQDCTYADQKAIDAETLQRVYDRVKFSRKGAKLKALAARGIGYHHSSLSAKEKQLVEILFRKGFIRVVTATGTLALGINMPCKSVVFAQNSVYLDALNYRQMSGRAGRRGQDLGGDVYFFDIPLPKIEKLIKSNVPELRGQFPLSISLILRLMCLASKGDDPEDARAKVLSVLKHSLLSFKQPRVLEMLKLYFLFSLQFLVKEGYLDQEGNPTGFAGLVSHLHYHEPSNLVFVGFLVRGLFHNLCQPTSKGSKHFSQDVMEKLVLVLANLFGRKYLPAQFQDATLKFYQSKVFLEDLPEDFKAALVEYNTNVTKDFASFLLIVSRLADMKQEHQLPLSKIDFSGEECEDSQLVSHLMSCKEGRVAISPFACLSGNSDGNLLHLETSDHVTLRTIGIDSTRAPVLWPQGFDHQGRRMQLNAYALDFYRHGSLTGLVQDNRMNEGAAYQLLKDFGLTIQSISVSLRELCENEEDNVVLAFEQLSETFFEKFNKI

Target ORF information:

RefSeq Version	XM_007116318.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 (DDX60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007116318.1

ORF Insert Sequence:

ATGGGTTTAG AAAAGTACCT GATGCTTTTA GAGAAAATGT CACAGTTGAT TTTGGATCAA 
ATGGCAAAAG CTGATTATGA CAGTCTGTTC AATGATTTTG TTGAGTCTGA GTTTTTTCTG 
ATTGATGGAG ACTCACTGCT GATGACGTAC GTCTGTGAGA AATCCTTGCG GCCTGAACAG 
AGCCTGCACT TCTTCTACCT GGTTGAACAC TACCTTGTGG ACCTAATTGG TAAAGGAGGA 
CAGTTTGCCA TCGTTTTCTT CGAGGATGCT GAGCATGCAT ACAACAACAT CCCTGAATTT 
CTTCCTCTGA GAATGGCATT AATTCTCCAC CTTCAGCATA ATACCAACAT TGATGTTCGA 
ACAGACTTTT CTGGGTGCTT CTCAGAAGAG TGGAAAACTT TCTTGGAAGA CCGTTACCCA 
TATTTTCTGA TCGTTTCCGA TGAAAGCTTG AGCCATCTAC AAGTATACCT TTTCAACTTT 
TTAATCATTC AGTCCTGGGC AATGAAGGTC AATGCCGTGC TCTTCTCGGG CCAAGCATCC 
GATGTCCTTC GACTTTATGC GTACTTTATG CCGAGCAAGT ACGAAAACCA GATGTTTTTC 
CAGAAGAATG AAAAGAACAT TGAGAATGCT TATAAAACCC TGATTAAAGA ATTGGAGGAA 
TGTAGAGTTT TAGCATTAGG ACATCTTTTT GGAAATTTAA AATGGAAAAA TATGCTGGAA 
GAGGCATGTG AAACTATTTC TCTGCTTAAG CAACTGTGGC CGGAAGGATC TGACATTCGG 
CAAGTTCTTT GTGTTACTTC ATGCTCCTTG TCTTTGAGTT TATACCATCA CTTTTTAGAA 
AACAGATCGA AGACCACCGA TGCTCAGAAA GCTAACATCC AGCAGATGGA GAGCGACTGT 
CCCACCCTGG AGGGGGTGAC AGACTGGTGT AGACTGCACT GTCTCAGCGT GGCCTTTCTC 
CTCCACTTAC CTCTTTCCCA GAGAGCATGT ACCAGATTCA TCACTTCCCA CTGGATTAAG 
GACATTCAGA CTTTCTTAAC AATGAAAAAG TGGTGTGAAT ACTTCATCCT AAGTAATATA 
GACGCTTTTG AATTTTGGAA TCTGAATTTA ACTCACCTTT CCGACTTAAG TGATGAGCTT 
TTATTGAAGA ATATAGCATT TTACTATGAA AATGAAAGCG TTCAAGGGCT ACATTTGAAT 
TTGGGAAATG TCATTATGAA AGATTATGAA CATCTCTGGG ATACTGTATC AAAGTTGGTC 
ACAAAGTTTG ATGTTGGAAA GCCATTTCCT CTGAGAACAA AACAATTTCC TTTTCTTGAA 
AAGGATCCAT CACCAATCAG AGATACCTCC AAGGAAAAGA TGGCTAATTT GGGTTTTATT 
CCAGTGTCAT GTCAGGTGGT TGATAAGTTT GCTGGGGACA TTTTGAAAGA TTTGCCTTTC 
CTAACGAGCG ATGATCCTAT TGTAACTTCA CTGGTCAAGC AGAAAGAATT TGATGAGCTT 
GTGCACTGGC ATTCTCACAG ACCCCTCAGT GATGATTATG ACAGGACAAA GGGCCACTTT 
GACGAAAAAT CTAGAGATCC TCGTCATCTT AGATCTGTGC AAATGTATCA TCGTTTTCAG 
CGATTTTATG GGAAGTCATT AGAATCAGTC TCTTCAAAAT TTGTTGTACC TCAAGCTATC 
AAGCCAAAGA AGAATTTCTG TGAACCCAAG AGCAAAAAGA TACAGGAGAC CAAGGCTGAA 
AAAATTACTA GAGAGAATAA GGAGAGGCTA TTTGCCAAGG AAGAACAAAA AGAAGAGAAA 
AAATGGAATG TTATGTCCTT TTCTATTGAG GAGGAAATGA AAGAGAATTT AAACTCTGGA 
ATAAAGAACC TAGAAGAGTT TTTGAAATCA TGTAAAAGTA GCTCAGTGAA ATTGCGAGTT 
GAAATGGTGG GGATTACTGC CTGCTTGAGA GCGTGGAAAG AACATTGCCG TGGTGAAGGT 
GAAACCACAA AAGATTTAAG TATAGCTGTT CAAATGATGA AAAGGATCTA CTCCGTGACG 
GAAAAATACC CAGAACTTTT GCAAGAAGCA GATTGGCAAC TCATAGCCAG ATGCCTTAGG 
TATTTAGGAT TTGAGGAGTT GGCAAGTTCT TTGCATCCAA CCCAGGTCAC AGGAAATGAC 
GTAAAGGAGA AGAAAAAGAA CAAATACTCA GTTGGCATGG GGCCAGCTCG GTTTCAGCTG 
CAGTACATGG GCCACTATTT GGTACGAGAT GAGAGGAAAG ATCCAGACCC CAGAGTCCAG 
GACTTTATTC CTGACACCTG GCAGCGAGAG CTCCTGGACG TTGTGGACAA GAACGAGTCT 
GCGGTCATAG TGGCCCCCAC GTCCTCAGGG AAGACCTACG CCTCCTACTA CTGCATGGAG 
AAGGTGCTGC GGGAGGGTGA CGACGGGGTG GTCGTGTACG TGGCCCCCAC CAAGGCCCTT 
GTCAATCAGG TGGCAGCAAC TGTTGAGAAT CGTTTTAACA AAAATCTGCC AGACGGTGAA 
ACTCTGTGTG GTGTTTTTAC ACGGGACTAT CGTCATGAGG CTCTAAACTC TCAGGTACTT 
ATCACAGTGC CTGCCTGCTT TGAAATTCTG CTGCTAGCTC CGCATCGCCA GGCGTGGGTG 
AAAAGGATCA GATACGTTAT ATTTGATGAG GTCCACTGTC TTGGTGGAGA AATTGGAGCA 
GAAGTCTGGG AGCATCTCCT TGTCATGATC CGCTGTCCCT TCCTGGCCCT TTCTGCTACC 
ATCAGCAACC CGGAGCATCT CACCGAGTGG CTACGTTCCA TAAAACAGTA CTGGAAACAA 
ACAGACAGTA CGATGGGAAA AAATATTGCT TCTTCAAGAA ATGCCGGCTG CCGTGCCAGT 
TCTCGCCAAG ACTACTTACT GATGAAGCAG TCGTATAAAG TGAGACTTGT GCTCTATGCA 
GAGAGATACA ATGATTTAGA GAAGCACATA TGTTCAGTAA ACAATCATGA CATTTCCTTT 
GACCATTTTC ACCCGTGCGC TGCGCTGACC ACGGACCATA TTGAAAAGTT TGGATTCCCT 
TCTGATCTTA CCCTTTCACC GCGAGAAATG ATCCAGCTTT ATGATGCCAT GTTTGAATTC 
TGGGAAAGTT GGCCCAGGGC TCAGGAGTTG TGTCCGGAGG AATTCGTTCA TTTTAAGAAT 
AAAATAGTCA TTCAAAAGAT GGATGCTAGA AAGTATGAAG AGAGCTTAAA GGCAGAACTG 
ACAGGCTGGG TGAAGAGCGG TCACGTCAAG GAGGCCAGAA TGGTCCTGAG GAATCTTCGT 
CCTCCTTCGG CTGTTAGCTC AGAAAACATG GCAGTTATGT TTCCACGTCT CGTTGAAAAG 
CTAAGGCAAA TGGAGAAGTT ACCTGCACTG TTTTTTTTAT TTAAATTAGG GGCCGTAGAG 
AGAAGCGCTG AAAGTGTGAA AACGTTCCTA GAGATAAAGC AACAGAACAG AAGGCCCCCC 
AAGGCTGATA AGGAGGCCTA TGACCTGGAG AACAAACTGC GAAAAGTTAG AAAATCCCTC 
GAGGCACAGA AGACAGTAGA TAAAAGAAGC CAGAAAAATC CCAGAAAAAT GGATCAAAGC 
CTAATCCATG AAGCTGAACA TGATAGTCTC CTCAGGGTTC TTGAGAAGAA CTTGGAGATC 
CCTCAGGACT GCACATATGC TGATCAGAAG GCCATAGATG CTGAGACTTT GCAGAGAGTG 
TATGACCGAG TGAAATTCTC AAGGAAAGGC GCCAAACTGA AGGCTTTGGC AGCAAGGGGC 
ATCGGATATC ATCACAGCTC TTTGAGCGCC AAAGAAAAGC AGTTAGTTGA GATTCTTTTC 
AGGAAGGGAT TTATTAGGGT GGTGACCGCT ACAGGGACAC TTGCTTTAGG AATCAACATG 
CCTTGTAAAT CTGTGGTTTT TGCTCAAAAC TCAGTCTATC TGGATGCTTT AAACTACAGA 
CAGATGTCTG GACGGGCCGG ACGACGAGGT CAAGACCTGG GGGGAGATGT GTATTTCTTT 
GATATTCCAC TCCCTAAAAT CGAAAAGCTC ATCAAATCCA ACGTCCCTGA ACTGAGAGGG 
CAGTTCCCTC TCAGCATATC CCTGATCCTG AGACTCATGT GCCTGGCTTC CAAGGGAGAT 
GACCCCGAGG ACGCTAGGGC AAAGGTGCTG TCCGTGCTCA AGCACTCACT GCTCTCCTTC 
AAGCAGCCCC GGGTGCTGGA GATGCTGAAA CTGTACTTTC TGTTCTCCCT GCAATTCCTG 
GTCAAGGAGG GCTATTTAGA TCAAGAAGGT AATCCTACAG GCTTTGCTGG ACTTGTGTCA 
CATTTGCATT ATCACGAGCC TTCTAATCTT GTTTTCGTCG GTTTTCTTGT AAGAGGCCTT 
TTCCATAATC TCTGTCAGCC GACCAGCAAA GGCTCAAAAC ATTTTTCTCA AGATGTTATG 
GAAAAGCTAG TGTTAGTATT GGCAAATCTG TTTGGAAGAA AATATCTTCC AGCACAGTTC 
CAAGATGCTA CGCTCAAGTT TTATCAATCC AAGGTGTTTC TGGAGGATCT CCCTGAGGAC 
TTCAAGGCTG CTCTAGTTGA GTATAACACA AATGTGACAA AGGACTTTGC CTCTTTCCTA 
CTGATTGTTT CCAGACTGGC TGATATGAAA CAAGAACATC AGCTCCCATT GTCAAAAATA 
GATTTCTCAG GTGAAGAGTG TGAAGACTCC CAACTTGTGT CTCACTTGAT GAGCTGCAAG 
GAAGGAAGAG TAGCCATTTC TCCATTTGCC TGTCTCTCTG GGAACTCTGA TGGAAATTTG 
CTTCATCTAG AAACTTCTGA CCATGTCACT CTGCGCACCA TTGGCATTGA TAGCACTCGG 
GCTCCTGTGC TGTGGCCACA GGGATTTGAT CACCAAGGAA GAAGAATGCA GCTCAATGCT 
TATGCACTCG ATTTCTATAG ACACGGTTCC TTGACAGGAT TGGTCCAGGA CAACAGGATG 
AATGAAGGAG CTGCTTATCA GTTGTTGAAA GATTTTGGGC TCACCATTCA ATCTATCAGT 
GTTTCTTTGC GTGAACTATG TGAAAATGAA GAGGACAACG TGGTCTTAGC ATTTGAACAA 
CTGAGTGAAA CCTTTTTTGA AAAATTTAAC AAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX60 ( XM_024130901.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023986669.1 Physeter catodon DExD/H-box helicase 60 (LOC102974719), mRNA.

Price & Availability↑

CloneID	OPh299792
Clone ID Related Accession (Same CDS sequence)	XM_024130901.1
Accession Version	XM_024130901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	probable ATP-dependent RNA helicase DDX60
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873585.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTAG AAAAGTACCT GATGCTTTTA GAGAAAATGT CACAGTTGAT TTTGGATCAA 
ATGGCAAAAG CTGATTATGA CAGTCTGTTC AATGATTTTG TTGAGTCTGA GTTTTTTCTG 
ATTGATGGAG ACTCACTGCT GATGACGTAC GTCTGTGAGA AATCCTTGCG GCCTGAACAG 
AGCCTGCACT TCTTCTACCT GGTTGAACAC TACCTTGTGG ACCTAATTGG TAAAGGAGGA 
CAGTTTGCCA TCGTTTTCTT CAAGGATGCT GAGCATGCAT ACAACAACAT CCCTGAATTT 
CTTCCTCTGA GAATGGCATT AATTCTCCAC CTTCAGCATA ATACCAACAT TGATGTTCGA 
ACAGACTTTT CTGGGTGCTT CTCACAAGAG TGGAAAACTT TCTTGGAAGA CCGTTACCCA 
TATTTTCTGA TCGTTTCCGA TGAAAGCTTG AGCCATCTAC AAGTATACCT TTTCAACTTT 
TTAATCATTC AGTCCTGGGC AATGAAGGTC AATGCCGTGC TCTTCTCGGG CCAAGCATCC 
GATGTCCTTC GACTTTATGC GTACTTTATG CCGAGCAAGT ACGAAAACCA GATGTTTTTC 
CAGAAGAATG AAAAGAACAT TGAGAATGCT TATAAAACCC TGATTAAAGA ATTGGAGGAA 
TGTAGAGTTT TAGCATTAGG ACATCTTTTT GGAAATTTAA AATGGAAAAA TATGCTGGAA 
GAGGCATGTG AAACTATTTC TCTGCTTAAG CAACTGTGGC CGGAAGGATC TGACATTCGG 
CAAGTTCTTT GTGTTACTTC ATGCTCCTTG TCTTTGAGTT TATACCATCA CTTTTTAGAA 
AACAGATCGA AGACCACCGA TGCTCAGAAA GCTAACATCC AGCAGATGGA GAGCGACTGT 
CCCACCCTGG AGGGGGTGAC AGACTGGTGT AGACTGCACT GTCTCAGCGT GGCCTTTCTC 
CTCCACTTAC CTCTTTCCCA GAGAGCATGT ACCAGATTCA TCACTTCCCA CTGGATTAAG 
GACATTCAGA CTTTCTTAAC AGTGAAAAAG TGGTGTGAAT ACTTCATCCT AAGTAATATA 
GACGCTTTTG AATTTTGGAA TCTGAATTTA ACTCACCTTT CCGACTTAAG TGATGAGCTT 
TTATTGAAGA ATATAGCATT TTACTATGAA AATGAAAGCG TTCAAGGGCT ACATTTGAAT 
TTGGGAAATG TCATTATGAA AGATTATGAA CATCTCTGGG ATACTGTATC AAAGTTGGTC 
ACAAAGTTTG ATGTTGGAAA GCCATTTCCT CTGAGAACAA AACAATTTCC TTTTCTTGAA 
AAGGATCCAT CACCAATCAG AGATACCTCC AAGGAAAAGA TGGCTAATTT GGGTTTTATT 
CCAGTGTCAT GTCAGGTGGT TGATAAGTTT GCTGGGGACA TTTTGAAAGA TTTGCCTTTC 
CTAACGAGCG ATGATCCTAT TGTAACTTCA CTGGTCAAGC AGAAAGAATT TGATGAGCTT 
GTGCACTGGC ATTCTCACAG ACCCCTCAGT GATGATTATG ACAGGACAAA GGGCCACTTT 
GACGAAAAAT CTAGAGATCC TCGTCATCTT AGATCTGTGC AAATGTATCA TCGTTTTCAG 
CGATTTTATG GGAAGTCATT AGAATCAGTC TCTTCAAAAT TTGTTGTACC TCAAGCTATC 
AAGCCAAAGA AGAATTTCTG TGAACCCAAG AGCAAAAAGA TACAGGAGAC CAAGGCTGAA 
AAAATTACTA GAGAGAATAA GGAGAGGCTA TTTGCCAAGG AAGAACAAAA AGAAGAGAAA 
AAATGGAATG TTATGTCCTT TTCTATTGAG GAGGAAATGA AAGAGAATTT AAACTCTGGA 
ATAAAGAACC TAGAAGAGTT TTTGAAATCA TGTAAAAGTA GCTCAGTGAA ATTGCGAGTT 
GAAATGGTGG GGATTACTGC CTGCTTGAGA GCGTGGAAAG AACATTGCCG TGGTGAAGGT 
GAAACCACAA AAGATTTAAG TATAGCTGTT CAAATGATGA AAAGGATCTA CTCCGTGACG 
GAAAAATACC CAGAACTTTT GCAAGAAGCA GATTGGCAAC TCATAGCCAG ATGCCTTAGG 
TATTTAGGAT TTGAGGAGTT GGCAAGTTCT TTGCATCCAA CCCAGGTCAC AGGAAATGAC 
GTAAAGGAGA AGAAAAAGAA CAAATACTCA GTTGGCATGG GGCCAGCTCG GTTTCAGCTG 
CAGTACATGG GCCACTATTT GGTACGAGAT GAGAGGAAAG ATCCAGACCC CAGAGTCCAG 
GACTTTATTC CTGACACCTG GCAGCGAGAG CTCCTGGACG TTGTGGACAA GAACGAGTCT 
GCGGTCATAG TGGCCCCCAC GTCCTCAGGG AAGACCTACG CCTCCTACTA CTGCATGGAG 
AAGGTGCTGC GGGAGGGTGA CGACGGGGTG GTCGTGTACG TGGCCCCCAC CAAGGCCCTT 
GTCAATCAGG TGGCAGCAAC TGTTGAGAAT CGTTTTAACA AAAATCTGCC AGACGGTGAA 
ACTCTGTGTG GTGTTTTTAC ACGGGACTAT CGTCATGAGG CTCTAAACTC TCAGGTACTT 
ATCACAGTGC CTGCCTGCTT TGAAATTCTG CTGCTAGCTC CGCATCGCCA GGCGTGGGTG 
AAAAGGATCA GATACGTTAT ATTTGATGAG GTCCACTGTC TTGGTGGAGA AATTGGAGCA 
GAAGTCTGGG AGCATCTCCT TGTCATGATC CGCTGTCCCT TCCTGGCCCT TTCTGCTACC 
ATCAGCAACC CGGAGCATCT CACCGAGTGG CTACGTTCCA TAAAACAGTA CTGGAAACAA 
ACAGACAGTA CGATGGGAAA AAATATTGCT TCTTCAAGAA ATGCCGGCTG CCGTGCCAGT 
TCTCGCCAAG ACTACTTACT GATGAAGCAG TCGTATAAAG TGAGACTTGT GCTCTATGCA 
GAGAGATACA ATGATTTAGA GAAGCACATA TGTTCAGTAA ACAATCATGA CATTTCCTTT 
GACCATTTTC ACCCGTGCGC TGCGCTGACC ACGGACCATA TTGAAAAGTT TGGATTCCCT 
TCTGATCTTA CCCTTTCACC GCGAGAAATG ATCCAGCTTT ATGATGCCAT GTTTGAATTC 
TGGGAAAGTT GGCCCAGGGC TCAGGAGTTG TGTCCGGAGG AATTCGTTCA TTTTAAGAAT 
AAAATAGTCA TTCAAAAGAT GGATGCTAGA AAGTATGAAG AGAGCTTAAA GGCAGAACTG 
ACAGGCTGGG TGAAGAGCGG TCACGTCAAG GAGGCCAGAA TGGTCCTGAG GAATCTTCGT 
CCTCCTTCGG CTGTTAGCTC AGAAAACATG GCAGTTATGT TTCCACGTCT CGTTGAAAAG 
CTAAGGCAAA TGGAGAAGTT ACCTGCACTG TTTTTTTTAT TTAAATTAGG GGCCGTAGAG 
AGAAGCGCTG AAAGTGTGAA AACGTTCCTA GAGATAAAGC AACAGAACAG AAGGCCCCCC 
AAGGCTGATA AGGAGGCCTA TGACCTGGAG AACAAACTGC GAAAAGTTAG AAAATCCCTC 
GAGGCACAGA AGACAGTAGA TAAAAGAAGC CAGAAAAATC CCAGAAAAAT GGATCAAAGC 
CTAATCCATG AAGCTGAACA TGATAGTCTC CTCAGGGTTC TTGAGAAGAA CTTGGAGATC 
CCTCAGGACT GCACATATGC TGATCAGAAG GCCATAGATG CTGAGACTTT GCAGAGAGTG 
TATGACCGAG TGAAATTCTC AAGGAAAGGC GCCAAACTGA AGGCTTTGGC AGCAAGGGGC 
ATCGGATATC ATCACAGCTC TTTGAGCGCC AAAGAAAAGC AGTTAGTTGA GATTCTTTTC 
AGGAAGGGAT TTATTAGGGT GGTGACCGCT ACAGGGACAC TTGCTTTAGG AATCAACATG 
CCTTGTAAAT CTGTGGTTTT TGCTCAAAAC TCAGTCTATC TGGATGCTTT AAACTACAGA 
CAGATGTCTG GACGGGCCGG ACGACGAGGT CAAGACCTGG GGGGAGATGT GTATTTCTTT 
GATATTCCAC TCCCTAAAAT CGAAAAGCTC ATCAAATCCA ACGTCCCTGA ACTGAGAGGG 
CAGTTCCCTC TCAGCATATC CCTGATCCTG AGACTCATGT GCCTGGCTTC CAAGGGAGAT 
GACCCCGAGG ACGCTAGGGC AAAGGTGCTG TCCGTGCTCA AGCACTCACT GCTCTCCTTC 
AAGCAGCCCC GGGTGCTGGA GATGCTGAAA CTGTACTTTC TGTTCTCCCT GCAATTCCTG 
GTCAAGGAGG GCTATTTAGA TCAAGAAGGT AATCCTACAG GCTTTGCTGG ACTTGTGTCA 
CATTTGCATT ATCACGAGCC TTCTAATCTT GTTTTCGTCG GTTTTCTTGT AAGAGGCCTT 
TTCCATAATC TCTGTCAGCC GACCAGCAAA GGCTCAAAAC ATTTTTCTCA AGATGTTATG 
GAAAAGCTAG TGTTAGTATT GGCAAATCTG TTTGGAAGAA AATATCTTCC AGCACAGTTC 
CAAGATGCTA CGCTCAAGTT TTATCAATCC AAGGTGTTTC TGGAGGATCT CCCTGAGGAC 
TTCAAGGCTG CTCTAGTTGA GTATAACACA AATGTGACAA AGGACTTTGC CTCTTTCCTA 
CTGATTGTTT CCAGACTGGC TGATATGAAA CAAGAACATC AGCTCCCATT GTCAAAAATA 
GATTTCTCAG GTGAAGAGTG TGAAGACTCC CAACTTGTGT CTCACTTGAT GAGCTGCAAG 
GAAGGAAGAG TAGCCATTTC TCCATTTGCC TGTCTCTCTG GGAACTCTGA TGGAAATTTG 
CTTCATCTAG AAACTTCTGA CCATGTCACT CTGCGCACCA TTGGCATTGA TAGCACTCGG 
GCTCCTGTGC TGTGGCCACA GGGATTTGAT CACCAAGGAA GAAGAATGCA GCTCAATGCT 
TATGCACTCG ATTTCTATAG ACACGGTTCC TTGACAGGAT TGGTCCAGGA CAACAGGATG 
AATGAAGGAG CTGCTTATCA GTTGTTGAAA GATTTTGGGC TCACCATTCA ATCTATCAGT 
GTTTCTTTGC GTGAACTATG TGAAAATGAA GAGGACAACG TGGTCTTAGC ATTTGAACAA 
CTGAGTGAAA CCTTTTTTGA AAAATTTAAC AAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023986669.1
CDS	186..5324
Translation	MGLEKYLMLLEKMSQLILDQMAKADYDSLFNDFVESEFFLIDGDSLLMTYVCEKSLRPEQSLHFFYLVEHYLVDLIGKGGQFAIVFFKDAEHAYNNIPEFLPLRMALILHLQHNTNIDVRTDFSGCFSQEWKTFLEDRYPYFLIVSDESLSHLQVYLFNFLIIQSWAMKVNAVLFSGQASDVLRLYAYFMPSKYENQMFFQKNEKNIENAYKTLIKELEECRVLALGHLFGNLKWKNMLEEACETISLLKQLWPEGSDIRQVLCVTSCSLSLSLYHHFLENRSKTTDAQKANIQQMESDCPTLEGVTDWCRLHCLSVAFLLHLPLSQRACTRFITSHWIKDIQTFLTVKKWCEYFILSNIDAFEFWNLNLTHLSDLSDELLLKNIAFYYENESVQGLHLNLGNVIMKDYEHLWDTVSKLVTKFDVGKPFPLRTKQFPFLEKDPSPIRDTSKEKMANLGFIPVSCQVVDKFAGDILKDLPFLTSDDPIVTSLVKQKEFDELVHWHSHRPLSDDYDRTKGHFDEKSRDPRHLRSVQMYHRFQRFYGKSLESVSSKFVVPQAIKPKKNFCEPKSKKIQETKAEKITRENKERLFAKEEQKEEKKWNVMSFSIEEEMKENLNSGIKNLEEFLKSCKSSSVKLRVEMVGITACLRAWKEHCRGEGETTKDLSIAVQMMKRIYSVTEKYPELLQEADWQLIARCLRYLGFEELASSLHPTQVTGNDVKEKKKNKYSVGMGPARFQLQYMGHYLVRDERKDPDPRVQDFIPDTWQRELLDVVDKNESAVIVAPTSSGKTYASYYCMEKVLREGDDGVVVYVAPTKALVNQVAATVENRFNKNLPDGETLCGVFTRDYRHEALNSQVLITVPACFEILLLAPHRQAWVKRIRYVIFDEVHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPEHLTEWLRSIKQYWKQTDSTMGKNIASSRNAGCRASSRQDYLLMKQSYKVRLVLYAERYNDLEKHICSVNNHDISFDHFHPCAALTTDHIEKFGFPSDLTLSPREMIQLYDAMFEFWESWPRAQELCPEEFVHFKNKIVIQKMDARKYEESLKAELTGWVKSGHVKEARMVLRNLRPPSAVSSENMAVMFPRLVEKLRQMEKLPALFFLFKLGAVERSAESVKTFLEIKQQNRRPPKADKEAYDLENKLRKVRKSLEAQKTVDKRSQKNPRKMDQSLIHEAEHDSLLRVLEKNLEIPQDCTYADQKAIDAETLQRVYDRVKFSRKGAKLKALAARGIGYHHSSLSAKEKQLVEILFRKGFIRVVTATGTLALGINMPCKSVVFAQNSVYLDALNYRQMSGRAGRRGQDLGGDVYFFDIPLPKIEKLIKSNVPELRGQFPLSISLILRLMCLASKGDDPEDARAKVLSVLKHSLLSFKQPRVLEMLKLYFLFSLQFLVKEGYLDQEGNPTGFAGLVSHLHYHEPSNLVFVGFLVRGLFHNLCQPTSKGSKHFSQDVMEKLVLVLANLFGRKYLPAQFQDATLKFYQSKVFLEDLPEDFKAALVEYNTNVTKDFASFLLIVSRLADMKQEHQLPLSKIDFSGEECEDSQLVSHLMSCKEGRVAISPFACLSGNSDGNLLHLETSDHVTLRTIGIDSTRAPVLWPQGFDHQGRRMQLNAYALDFYRHGSLTGLVQDNRMNEGAAYQLLKDFGLTIQSISVSLRELCENEEDNVVLAFEQLSETFFEKFNKI

Target ORF information:

RefSeq Version	XM_024130901.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon DExD/H-box helicase 60 (LOC102974719), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024130901.1

ORF Insert Sequence:

ATGGGTTTAG AAAAGTACCT GATGCTTTTA GAGAAAATGT CACAGTTGAT TTTGGATCAA 
ATGGCAAAAG CTGATTATGA CAGTCTGTTC AATGATTTTG TTGAGTCTGA GTTTTTTCTG 
ATTGATGGAG ACTCACTGCT GATGACGTAC GTCTGTGAGA AATCCTTGCG GCCTGAACAG 
AGCCTGCACT TCTTCTACCT GGTTGAACAC TACCTTGTGG ACCTAATTGG TAAAGGAGGA 
CAGTTTGCCA TCGTTTTCTT CAAGGATGCT GAGCATGCAT ACAACAACAT CCCTGAATTT 
CTTCCTCTGA GAATGGCATT AATTCTCCAC CTTCAGCATA ATACCAACAT TGATGTTCGA 
ACAGACTTTT CTGGGTGCTT CTCACAAGAG TGGAAAACTT TCTTGGAAGA CCGTTACCCA 
TATTTTCTGA TCGTTTCCGA TGAAAGCTTG AGCCATCTAC AAGTATACCT TTTCAACTTT 
TTAATCATTC AGTCCTGGGC AATGAAGGTC AATGCCGTGC TCTTCTCGGG CCAAGCATCC 
GATGTCCTTC GACTTTATGC GTACTTTATG CCGAGCAAGT ACGAAAACCA GATGTTTTTC 
CAGAAGAATG AAAAGAACAT TGAGAATGCT TATAAAACCC TGATTAAAGA ATTGGAGGAA 
TGTAGAGTTT TAGCATTAGG ACATCTTTTT GGAAATTTAA AATGGAAAAA TATGCTGGAA 
GAGGCATGTG AAACTATTTC TCTGCTTAAG CAACTGTGGC CGGAAGGATC TGACATTCGG 
CAAGTTCTTT GTGTTACTTC ATGCTCCTTG TCTTTGAGTT TATACCATCA CTTTTTAGAA 
AACAGATCGA AGACCACCGA TGCTCAGAAA GCTAACATCC AGCAGATGGA GAGCGACTGT 
CCCACCCTGG AGGGGGTGAC AGACTGGTGT AGACTGCACT GTCTCAGCGT GGCCTTTCTC 
CTCCACTTAC CTCTTTCCCA GAGAGCATGT ACCAGATTCA TCACTTCCCA CTGGATTAAG 
GACATTCAGA CTTTCTTAAC AGTGAAAAAG TGGTGTGAAT ACTTCATCCT AAGTAATATA 
GACGCTTTTG AATTTTGGAA TCTGAATTTA ACTCACCTTT CCGACTTAAG TGATGAGCTT 
TTATTGAAGA ATATAGCATT TTACTATGAA AATGAAAGCG TTCAAGGGCT ACATTTGAAT 
TTGGGAAATG TCATTATGAA AGATTATGAA CATCTCTGGG ATACTGTATC AAAGTTGGTC 
ACAAAGTTTG ATGTTGGAAA GCCATTTCCT CTGAGAACAA AACAATTTCC TTTTCTTGAA 
AAGGATCCAT CACCAATCAG AGATACCTCC AAGGAAAAGA TGGCTAATTT GGGTTTTATT 
CCAGTGTCAT GTCAGGTGGT TGATAAGTTT GCTGGGGACA TTTTGAAAGA TTTGCCTTTC 
CTAACGAGCG ATGATCCTAT TGTAACTTCA CTGGTCAAGC AGAAAGAATT TGATGAGCTT 
GTGCACTGGC ATTCTCACAG ACCCCTCAGT GATGATTATG ACAGGACAAA GGGCCACTTT 
GACGAAAAAT CTAGAGATCC TCGTCATCTT AGATCTGTGC AAATGTATCA TCGTTTTCAG 
CGATTTTATG GGAAGTCATT AGAATCAGTC TCTTCAAAAT TTGTTGTACC TCAAGCTATC 
AAGCCAAAGA AGAATTTCTG TGAACCCAAG AGCAAAAAGA TACAGGAGAC CAAGGCTGAA 
AAAATTACTA GAGAGAATAA GGAGAGGCTA TTTGCCAAGG AAGAACAAAA AGAAGAGAAA 
AAATGGAATG TTATGTCCTT TTCTATTGAG GAGGAAATGA AAGAGAATTT AAACTCTGGA 
ATAAAGAACC TAGAAGAGTT TTTGAAATCA TGTAAAAGTA GCTCAGTGAA ATTGCGAGTT 
GAAATGGTGG GGATTACTGC CTGCTTGAGA GCGTGGAAAG AACATTGCCG TGGTGAAGGT 
GAAACCACAA AAGATTTAAG TATAGCTGTT CAAATGATGA AAAGGATCTA CTCCGTGACG 
GAAAAATACC CAGAACTTTT GCAAGAAGCA GATTGGCAAC TCATAGCCAG ATGCCTTAGG 
TATTTAGGAT TTGAGGAGTT GGCAAGTTCT TTGCATCCAA CCCAGGTCAC AGGAAATGAC 
GTAAAGGAGA AGAAAAAGAA CAAATACTCA GTTGGCATGG GGCCAGCTCG GTTTCAGCTG 
CAGTACATGG GCCACTATTT GGTACGAGAT GAGAGGAAAG ATCCAGACCC CAGAGTCCAG 
GACTTTATTC CTGACACCTG GCAGCGAGAG CTCCTGGACG TTGTGGACAA GAACGAGTCT 
GCGGTCATAG TGGCCCCCAC GTCCTCAGGG AAGACCTACG CCTCCTACTA CTGCATGGAG 
AAGGTGCTGC GGGAGGGTGA CGACGGGGTG GTCGTGTACG TGGCCCCCAC CAAGGCCCTT 
GTCAATCAGG TGGCAGCAAC TGTTGAGAAT CGTTTTAACA AAAATCTGCC AGACGGTGAA 
ACTCTGTGTG GTGTTTTTAC ACGGGACTAT CGTCATGAGG CTCTAAACTC TCAGGTACTT 
ATCACAGTGC CTGCCTGCTT TGAAATTCTG CTGCTAGCTC CGCATCGCCA GGCGTGGGTG 
AAAAGGATCA GATACGTTAT ATTTGATGAG GTCCACTGTC TTGGTGGAGA AATTGGAGCA 
GAAGTCTGGG AGCATCTCCT TGTCATGATC CGCTGTCCCT TCCTGGCCCT TTCTGCTACC 
ATCAGCAACC CGGAGCATCT CACCGAGTGG CTACGTTCCA TAAAACAGTA CTGGAAACAA 
ACAGACAGTA CGATGGGAAA AAATATTGCT TCTTCAAGAA ATGCCGGCTG CCGTGCCAGT 
TCTCGCCAAG ACTACTTACT GATGAAGCAG TCGTATAAAG TGAGACTTGT GCTCTATGCA 
GAGAGATACA ATGATTTAGA GAAGCACATA TGTTCAGTAA ACAATCATGA CATTTCCTTT 
GACCATTTTC ACCCGTGCGC TGCGCTGACC ACGGACCATA TTGAAAAGTT TGGATTCCCT 
TCTGATCTTA CCCTTTCACC GCGAGAAATG ATCCAGCTTT ATGATGCCAT GTTTGAATTC 
TGGGAAAGTT GGCCCAGGGC TCAGGAGTTG TGTCCGGAGG AATTCGTTCA TTTTAAGAAT 
AAAATAGTCA TTCAAAAGAT GGATGCTAGA AAGTATGAAG AGAGCTTAAA GGCAGAACTG 
ACAGGCTGGG TGAAGAGCGG TCACGTCAAG GAGGCCAGAA TGGTCCTGAG GAATCTTCGT 
CCTCCTTCGG CTGTTAGCTC AGAAAACATG GCAGTTATGT TTCCACGTCT CGTTGAAAAG 
CTAAGGCAAA TGGAGAAGTT ACCTGCACTG TTTTTTTTAT TTAAATTAGG GGCCGTAGAG 
AGAAGCGCTG AAAGTGTGAA AACGTTCCTA GAGATAAAGC AACAGAACAG AAGGCCCCCC 
AAGGCTGATA AGGAGGCCTA TGACCTGGAG AACAAACTGC GAAAAGTTAG AAAATCCCTC 
GAGGCACAGA AGACAGTAGA TAAAAGAAGC CAGAAAAATC CCAGAAAAAT GGATCAAAGC 
CTAATCCATG AAGCTGAACA TGATAGTCTC CTCAGGGTTC TTGAGAAGAA CTTGGAGATC 
CCTCAGGACT GCACATATGC TGATCAGAAG GCCATAGATG CTGAGACTTT GCAGAGAGTG 
TATGACCGAG TGAAATTCTC AAGGAAAGGC GCCAAACTGA AGGCTTTGGC AGCAAGGGGC 
ATCGGATATC ATCACAGCTC TTTGAGCGCC AAAGAAAAGC AGTTAGTTGA GATTCTTTTC 
AGGAAGGGAT TTATTAGGGT GGTGACCGCT ACAGGGACAC TTGCTTTAGG AATCAACATG 
CCTTGTAAAT CTGTGGTTTT TGCTCAAAAC TCAGTCTATC TGGATGCTTT AAACTACAGA 
CAGATGTCTG GACGGGCCGG ACGACGAGGT CAAGACCTGG GGGGAGATGT GTATTTCTTT 
GATATTCCAC TCCCTAAAAT CGAAAAGCTC ATCAAATCCA ACGTCCCTGA ACTGAGAGGG 
CAGTTCCCTC TCAGCATATC CCTGATCCTG AGACTCATGT GCCTGGCTTC CAAGGGAGAT 
GACCCCGAGG ACGCTAGGGC AAAGGTGCTG TCCGTGCTCA AGCACTCACT GCTCTCCTTC 
AAGCAGCCCC GGGTGCTGGA GATGCTGAAA CTGTACTTTC TGTTCTCCCT GCAATTCCTG 
GTCAAGGAGG GCTATTTAGA TCAAGAAGGT AATCCTACAG GCTTTGCTGG ACTTGTGTCA 
CATTTGCATT ATCACGAGCC TTCTAATCTT GTTTTCGTCG GTTTTCTTGT AAGAGGCCTT 
TTCCATAATC TCTGTCAGCC GACCAGCAAA GGCTCAAAAC ATTTTTCTCA AGATGTTATG 
GAAAAGCTAG TGTTAGTATT GGCAAATCTG TTTGGAAGAA AATATCTTCC AGCACAGTTC 
CAAGATGCTA CGCTCAAGTT TTATCAATCC AAGGTGTTTC TGGAGGATCT CCCTGAGGAC 
TTCAAGGCTG CTCTAGTTGA GTATAACACA AATGTGACAA AGGACTTTGC CTCTTTCCTA 
CTGATTGTTT CCAGACTGGC TGATATGAAA CAAGAACATC AGCTCCCATT GTCAAAAATA 
GATTTCTCAG GTGAAGAGTG TGAAGACTCC CAACTTGTGT CTCACTTGAT GAGCTGCAAG 
GAAGGAAGAG TAGCCATTTC TCCATTTGCC TGTCTCTCTG GGAACTCTGA TGGAAATTTG 
CTTCATCTAG AAACTTCTGA CCATGTCACT CTGCGCACCA TTGGCATTGA TAGCACTCGG 
GCTCCTGTGC TGTGGCCACA GGGATTTGAT CACCAAGGAA GAAGAATGCA GCTCAATGCT 
TATGCACTCG ATTTCTATAG ACACGGTTCC TTGACAGGAT TGGTCCAGGA CAACAGGATG 
AATGAAGGAG CTGCTTATCA GTTGTTGAAA GATTTTGGGC TCACCATTCA ATCTATCAGT 
GTTTCTTTGC GTGAACTATG TGAAAATGAA GAGGACAACG TGGTCTTAGC ATTTGAACAA 
CTGAGTGAAA CCTTTTTTGA AAAATTTAAC AAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX60 ( XM_028494281.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_028350082.1 Physeter catodon DExD/H-box helicase 60 (LOC102974719), transcript variant X1, mRNA.

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CloneID	OPh331883
Clone ID Related Accession (Same CDS sequence)	XM_028494281.1
Accession Version	XM_028494281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	probable ATP-dependent RNA helicase DDX60 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041222.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTAG AAAAGTACCT GATGCTTTTA GAGAAAATGT CACAGTTGAT TTTGGATCAA 
ATGGCAAAAG CTGATTATGA CAGTCTGTTC AATGATTTTG TTGAGTCTGA GTTTTTTCTG 
ATTGATGGAG ACTCACTGCT GATGACGTAC GTCTGTGAGA AATCCTTGCG GCCTGAACAG 
AGCCTGCACT TCTTCTACCT GGTTGAACAC TACCTTGTGG ACCTAATTGG TAAAGGAGGA 
CAGTTTGCCA TCGTTTTCTT CAAGGATGCT GAGCATGCAT ACAACAACAT CCCTGAATTT 
CTTCCTCTGA GAATGGCATT AATTCTCCAC CTTCAGCATA ATACCAACAT TGATGTTCGA 
ACAGACTTTT CTGGGTGCTT CTCACAAGAG TGGAAAACTT TCTTGGAAGA CCGTTACCCA 
TATTTTCTGA TCGTTTCCGA TGAAAGCTTG AGCCATCTAC AAGTATACCT TTTCAACTTT 
TTAATCATTC AGTCCTGGGC AATGAAGGTC AATGCCGTGC TCTTCTCGGG CCAAGCATCC 
GATGTCCTTC GACTTTATGC GTACTTTATG CCGAGCAAGT ACGAAAACCA GATGTTTTTC 
CAGAAGAATG AAAAGAACAT TGAGAATGCT TATAAAACCC TGATTAAAGA ATTGGAGGAA 
TGTAGAGTTT TAGCATTAGG ACATCTTTTT GGAAATTTAA AATGGAAAAA TATGCTGGAA 
GAGGCATGTG AAACTATTTC TCTGCTTAAG CAACTGTGGC CGGAAGGATC TGACATTCGG 
CAAGTTCTTT GTGTTACTTC ATGCTCCTTG TCTTTGAGTT TATACCATCA CTTTTTAGAA 
AACAGATCGA AGACCACCGA TGCTCAGAAA GCTAACATCC AGCAGCAGAT GGAGAGCGAC 
TGTCCCACCC TGGAGGGGGT GACAGACTGG TGTAGACTGC ACTGTCTCAG CGTGGCCTTT 
CTCCTCCACT TACCTCTTTC CCAGAGAGCA TGTACCAGAT TCATCACTTC CCACTGGATT 
AAGGACATTC AGACTTTCTT AACAGTGAAA AAGTGGTGTG AATACTTCAT CCTAAGTAAT 
ATAGACGCTT TTGAATTTTG GAATCTGAAT TTAACTCACC TTTCCGACTT AAGTGATGAG 
CTTTTATTGA AGAATATAGC ATTTTACTAT GAAAATGAAA GCGTTCAAGG GCTACATTTG 
AATTTGGGAA ATGTCATTAT GAAAGATTAT GAACATCTCT GGGATACTGT ATCAAAGTTG 
GTCACAAAGT TTGATGTTGG AAAGCCATTT CCTCTGAGAA CAAAACAATT TCCTTTTCTT 
GAAAAGGATC CATCACCAAT CAGAGATACC TCCAAGGAAA AGATGGCTAA TTTGGGTTTT 
ATTCCAGTGT CATGTCAGGT GGTTGATAAG TTTGCTGGGG ACATTTTGAA AGATTTGCCT 
TTCCTAACGA GCGATGATCC TATTGTAACT TCACTGGTCA AGCAGAAAGA ATTTGATGAG 
CTTGTGCACT GGCATTCTCA CAGACCCCTC AGTGATGATT ATGACAGGAC AAAGGGCCAC 
TTTGACGAAA AATCTAGAGA TCCTCGTCAT CTTAGATCTG TGCAAATGTA TCATCGTTTT 
CAGCGATTTT ATGGGAAGTC ATTAGAATCA GTCTCTTCAA AATTTGTTGT ACCTCAAGCT 
ATCAAGCCAA AGAAGAATTT CTGTGAACCC AAGAGCAAAA AGATACAGGA GACCAAGGCT 
GAAAAAATTA CTAGAGAGAA TAAGGAGAGG CTATTTGCCA AGGAAGAACA AAAAGAAGAG 
AAAAAATGGA ATGTTATGTC CTTTTCTATT GAGGAGGAAA TGAAAGAGAA TTTAAACTCT 
GGAATAAAGA ACCTAGAAGA GTTTTTGAAA TCATGTAAAA GTAGCTCAGT GAAATTGCGA 
GTTGAAATGG TGGGGATTAC TGCCTGCTTG AGAGCGTGGA AAGAACATTG CCGTGGTGAA 
GGTGAAACCA CAAAAGATTT AAGTATAGCT GTTCAAATGA TGAAAAGGAT CTACTCCGTG 
ACGGAAAAAT ACCCAGAACT TTTGCAAGAA GCAGATTGGC AACTCATAGC CAGATGCCTT 
AGGTATTTAG GATTTGAGGA GTTGGCAAGT TCTTTGCATC CAACCCAGGT CACAGGAAAT 
GACGTAAAGG AGAAGAAAAA GAACAAATAC TCAGTTGGCA TGGGGCCAGC TCGGTTTCAG 
CTGCAGTACA TGGGCCACTA TTTGGTACGA GATGAGAGGA AAGATCCAGA CCCCAGAGTC 
CAGGACTTTA TTCCTGACAC CTGGCAGCGA GAGCTCCTGG ACGTTGTGGA CAAGAACGAG 
TCTGCGGTCA TAGTGGCCCC CACGTCCTCA GGGAAGACCT ACGCCTCCTA CTACTGCATG 
GAGAAGGTGC TGCGGGAGGG TGACGACGGG GTGGTCGTGT ACGTGGCCCC CACCAAGGCC 
CTTGTCAATC AGGTGGCAGC AACTGTTGAG AATCGTTTTA ACAAAAATCT GCCAGACGGT 
GAAACTCTGT GTGGTGTTTT TACACGGGAC TATCGTCATG AGGCTCTAAA CTCTCAGGTA 
CTTATCACAG TGCCTGCCTG CTTTGAAATT CTGCTGCTAG CTCCGCATCG CCAGGCGTGG 
GTGAAAAGGA TCAGATACGT TATATTTGAT GAGGTCCACT GTCTTGGTGG AGAAATTGGA 
GCAGAAGTCT GGGAGCATCT CCTTGTCATG ATCCGCTGTC CCTTCCTGGC CCTTTCTGCT 
ACCATCAGCA ACCCGGAGCA TCTCACCGAG TGGCTACGTT CCATAAAACA GTACTGGAAA 
CAAACAGACA GTACGATGGG AAAAAATATT GCTTCTTCAA GAAATGCCGG CTGCCGTGCC 
AGTTCTCGCC AAGACTACTT ACTGATGAAG CAGTCGTATA AAGTGAGACT TGTGCTCTAT 
GCAGAGAGAT ACAATGATTT AGAGAAGCAC ATATGTTCAG TAAACAATCA TGACATTTCC 
TTTGACCATT TTCACCCGTG CGCTGCGCTG ACCACGGACC ATATTGAAAA GTTTGGATTC 
CCTTCTGATC TTACCCTTTC ACCGCGAGAA ATGATCCAGC TTTATGATGC CATGTTTGAA 
TTCTGGGAAA GTTGGCCCAG GGCTCAGGAG TTGTGTCCGG AGGAATTCGT TCATTTTAAG 
AATAAAATAG TCATTCAAAA GATGGATGCT AGAAAGTATG AAGAGAGCTT AAAGGCAGAA 
CTGACAGGCT GGGTGAAGAG CGGTCACGTC AAGGAGGCCA GAATGGTCCT GAGGAATCTT 
CGTCCTCCTT CGGCTGTTAG CTCAGAAAAC ATGGCAGTTA TGTTTCCACG TCTCGTTGAA 
AAGCTAAGGC AAATGGAGAA GTTACCTGCA CTGTTTTTTT TATTTAAATT AGGGGCCGTA 
GAGAGAAGCG CTGAAAGTGT GAAAACGTTC CTAGAGATAA AGCAACAGAA CAGAAGGCCC 
CCCAAGGCTG ATAAGGAGGC CTATGACCTG GAGAACAAAC TGCGAAAAGT TAGAAAATCC 
CTCGAGGCAC AGAAGACAGT AGATAAAAGA AGCCAGAAAA ATCCCAGAAA AATGGATCAA 
AGCCTAATCC ATGAAGCTGA ACATGATAGT CTCCTCAGGG TTCTTGAGAA GAACTTGGAG 
ATCCCTCAGG ACTGCACATA TGCTGATCAG AAGGCCATAG ATGCTGAGAC TTTGCAGAGA 
GTGTATGACC GAGTGAAATT CTCAAGGAAA GGCGCCAAAC TGAAGGCTTT GGCAGCAAGG 
GGCATCGGAT ATCATCACAG CTCTTTGAGC GCCAAAGAAA AGCAGTTAGT TGAGATTCTT 
TTCAGGAAGG GATTTATTAG GGTGGTGACC GCTACAGGGA CACTTGCTTT AGGAATCAAC 
ATGCCTTGTA AATCTGTGGT TTTTGCTCAA AACTCAGTCT ATCTGGATGC TTTAAACTAC 
AGACAGATGT CTGGACGGGC CGGACGACGA GGTCAAGACC TGGGGGGAGA TGTGTATTTC 
TTTGATATTC CACTCCCTAA AATCGAAAAG CTCATCAAAT CCAACGTCCC TGAACTGAGA 
GGGCAGTTCC CTCTCAGCAT ATCCCTGATC CTGAGACTCA TGTGCCTGGC TTCCAAGGGA 
GATGACCCCG AGGACGCTAG GGCAAAGGTG CTGTCCGTGC TCAAGCACTC ACTGCTCTCC 
TTCAAGCAGC CCCGGGTGCT GGAGATGCTG AAACTGTACT TTCTGTTCTC CCTGCAATTC 
CTGGTCAAGG AGGGCTATTT AGATCAAGAA GGTAATCCTA CAGGCTTTGC TGGACTTGTG 
TCACATTTGC ATTATCACGA GCCTTCTAAT CTTGTTTTCG TCGGTTTTCT TGTAAGAGGC 
CTTTTCCATA ATCTCTGTCA GCCGACCAGC AAAGGCTCAA AACATTTTTC TCAAGATGTT 
ATGGAAAAGC TAGTGTTAGT ATTGGCAAAT CTGTTTGGAA GAAAATATCT TCCAGCACAG 
TTCCAAGATG CTACGCTCAA GTTTTATCAA TCCAAGGTGT TTCTGGAGGA TCTCCCTGAG 
GACTTCAAGG CTGCTCTAGT TGAGTATAAC ACAAATGTGA CAAAGGACTT TGCCTCTTTC 
CTACTGATTG TTTCCAGACT GGCTGATATG AAACAAGAAC ATCAGCTCCC ATTGTCAAAA 
ATAGATTTCT CAGGTGAAGA GTGTGAAGAC TCCCAACTTG TGTCTCACTT GATGAGCTGC 
AAGGAAGGAA GAGTAGCCAT TTCTCCATTT GCCTGTCTCT CTGGGAACTC TGATGGAAAT 
TTGCTTCATC TAGAAACTTC TGACCATGTC ACTCTGCGCA CCATTGGCAT TGATAGCACT 
CGGGCTCCTG TGCTGTGGCC ACAGGGATTT GATCACCAAG GAAGAAGAAT GCAGCTCAAT 
GCTTATGCAC TCGATTTCTA TAGACACGGT TCCTTGACAG GATTGGTCCA GGACAACAGG 
ATGAATGAAG GAGCTGCTTA TCAGTTGTTG AAAGATTTTG GGCTCACCAT TCAATCTATC 
AGTGGGATTT ACTTAGCTCT GCCTCCCTGG GGAAAGCCCT TCTTAAGATA CCGACTCTCA 
GTAGAATGGG TGAGTTTTAT GTGTATCTAT ATTTCCATTT ACAGTGTTTC TTTGCGTGAA 
CTATGTGAAA ATGAAGAGGA CAACGTGGTC TTAGCATTTG AACAACTGAG TGAAACCTTT 
TTTGAAAAAT TTAACAAGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028350082.1
CDS	147..5390
Translation	MGLEKYLMLLEKMSQLILDQMAKADYDSLFNDFVESEFFLIDGDSLLMTYVCEKSLRPEQSLHFFYLVEHYLVDLIGKGGQFAIVFFKDAEHAYNNIPEFLPLRMALILHLQHNTNIDVRTDFSGCFSQEWKTFLEDRYPYFLIVSDESLSHLQVYLFNFLIIQSWAMKVNAVLFSGQASDVLRLYAYFMPSKYENQMFFQKNEKNIENAYKTLIKELEECRVLALGHLFGNLKWKNMLEEACETISLLKQLWPEGSDIRQVLCVTSCSLSLSLYHHFLENRSKTTDAQKANIQQQMESDCPTLEGVTDWCRLHCLSVAFLLHLPLSQRACTRFITSHWIKDIQTFLTVKKWCEYFILSNIDAFEFWNLNLTHLSDLSDELLLKNIAFYYENESVQGLHLNLGNVIMKDYEHLWDTVSKLVTKFDVGKPFPLRTKQFPFLEKDPSPIRDTSKEKMANLGFIPVSCQVVDKFAGDILKDLPFLTSDDPIVTSLVKQKEFDELVHWHSHRPLSDDYDRTKGHFDEKSRDPRHLRSVQMYHRFQRFYGKSLESVSSKFVVPQAIKPKKNFCEPKSKKIQETKAEKITRENKERLFAKEEQKEEKKWNVMSFSIEEEMKENLNSGIKNLEEFLKSCKSSSVKLRVEMVGITACLRAWKEHCRGEGETTKDLSIAVQMMKRIYSVTEKYPELLQEADWQLIARCLRYLGFEELASSLHPTQVTGNDVKEKKKNKYSVGMGPARFQLQYMGHYLVRDERKDPDPRVQDFIPDTWQRELLDVVDKNESAVIVAPTSSGKTYASYYCMEKVLREGDDGVVVYVAPTKALVNQVAATVENRFNKNLPDGETLCGVFTRDYRHEALNSQVLITVPACFEILLLAPHRQAWVKRIRYVIFDEVHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPEHLTEWLRSIKQYWKQTDSTMGKNIASSRNAGCRASSRQDYLLMKQSYKVRLVLYAERYNDLEKHICSVNNHDISFDHFHPCAALTTDHIEKFGFPSDLTLSPREMIQLYDAMFEFWESWPRAQELCPEEFVHFKNKIVIQKMDARKYEESLKAELTGWVKSGHVKEARMVLRNLRPPSAVSSENMAVMFPRLVEKLRQMEKLPALFFLFKLGAVERSAESVKTFLEIKQQNRRPPKADKEAYDLENKLRKVRKSLEAQKTVDKRSQKNPRKMDQSLIHEAEHDSLLRVLEKNLEIPQDCTYADQKAIDAETLQRVYDRVKFSRKGAKLKALAARGIGYHHSSLSAKEKQLVEILFRKGFIRVVTATGTLALGINMPCKSVVFAQNSVYLDALNYRQMSGRAGRRGQDLGGDVYFFDIPLPKIEKLIKSNVPELRGQFPLSISLILRLMCLASKGDDPEDARAKVLSVLKHSLLSFKQPRVLEMLKLYFLFSLQFLVKEGYLDQEGNPTGFAGLVSHLHYHEPSNLVFVGFLVRGLFHNLCQPTSKGSKHFSQDVMEKLVLVLANLFGRKYLPAQFQDATLKFYQSKVFLEDLPEDFKAALVEYNTNVTKDFASFLLIVSRLADMKQEHQLPLSKIDFSGEECEDSQLVSHLMSCKEGRVAISPFACLSGNSDGNLLHLETSDHVTLRTIGIDSTRAPVLWPQGFDHQGRRMQLNAYALDFYRHGSLTGLVQDNRMNEGAAYQLLKDFGLTIQSISGIYLALPPWGKPFLRYRLSVEWVSFMCIYISIYSVSLRELCENEEDNVVLAFEQLSETFFEKFNKI

Target ORF information:

RefSeq Version	XM_028494281.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon DExD/H-box helicase 60 (LOC102974719), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028494281.1

ORF Insert Sequence:

ATGGGTTTAG AAAAGTACCT GATGCTTTTA GAGAAAATGT CACAGTTGAT TTTGGATCAA 
ATGGCAAAAG CTGATTATGA CAGTCTGTTC AATGATTTTG TTGAGTCTGA GTTTTTTCTG 
ATTGATGGAG ACTCACTGCT GATGACGTAC GTCTGTGAGA AATCCTTGCG GCCTGAACAG 
AGCCTGCACT TCTTCTACCT GGTTGAACAC TACCTTGTGG ACCTAATTGG TAAAGGAGGA 
CAGTTTGCCA TCGTTTTCTT CAAGGATGCT GAGCATGCAT ACAACAACAT CCCTGAATTT 
CTTCCTCTGA GAATGGCATT AATTCTCCAC CTTCAGCATA ATACCAACAT TGATGTTCGA 
ACAGACTTTT CTGGGTGCTT CTCACAAGAG TGGAAAACTT TCTTGGAAGA CCGTTACCCA 
TATTTTCTGA TCGTTTCCGA TGAAAGCTTG AGCCATCTAC AAGTATACCT TTTCAACTTT 
TTAATCATTC AGTCCTGGGC AATGAAGGTC AATGCCGTGC TCTTCTCGGG CCAAGCATCC 
GATGTCCTTC GACTTTATGC GTACTTTATG CCGAGCAAGT ACGAAAACCA GATGTTTTTC 
CAGAAGAATG AAAAGAACAT TGAGAATGCT TATAAAACCC TGATTAAAGA ATTGGAGGAA 
TGTAGAGTTT TAGCATTAGG ACATCTTTTT GGAAATTTAA AATGGAAAAA TATGCTGGAA 
GAGGCATGTG AAACTATTTC TCTGCTTAAG CAACTGTGGC CGGAAGGATC TGACATTCGG 
CAAGTTCTTT GTGTTACTTC ATGCTCCTTG TCTTTGAGTT TATACCATCA CTTTTTAGAA 
AACAGATCGA AGACCACCGA TGCTCAGAAA GCTAACATCC AGCAGCAGAT GGAGAGCGAC 
TGTCCCACCC TGGAGGGGGT GACAGACTGG TGTAGACTGC ACTGTCTCAG CGTGGCCTTT 
CTCCTCCACT TACCTCTTTC CCAGAGAGCA TGTACCAGAT TCATCACTTC CCACTGGATT 
AAGGACATTC AGACTTTCTT AACAGTGAAA AAGTGGTGTG AATACTTCAT CCTAAGTAAT 
ATAGACGCTT TTGAATTTTG GAATCTGAAT TTAACTCACC TTTCCGACTT AAGTGATGAG 
CTTTTATTGA AGAATATAGC ATTTTACTAT GAAAATGAAA GCGTTCAAGG GCTACATTTG 
AATTTGGGAA ATGTCATTAT GAAAGATTAT GAACATCTCT GGGATACTGT ATCAAAGTTG 
GTCACAAAGT TTGATGTTGG AAAGCCATTT CCTCTGAGAA CAAAACAATT TCCTTTTCTT 
GAAAAGGATC CATCACCAAT CAGAGATACC TCCAAGGAAA AGATGGCTAA TTTGGGTTTT 
ATTCCAGTGT CATGTCAGGT GGTTGATAAG TTTGCTGGGG ACATTTTGAA AGATTTGCCT 
TTCCTAACGA GCGATGATCC TATTGTAACT TCACTGGTCA AGCAGAAAGA ATTTGATGAG 
CTTGTGCACT GGCATTCTCA CAGACCCCTC AGTGATGATT ATGACAGGAC AAAGGGCCAC 
TTTGACGAAA AATCTAGAGA TCCTCGTCAT CTTAGATCTG TGCAAATGTA TCATCGTTTT 
CAGCGATTTT ATGGGAAGTC ATTAGAATCA GTCTCTTCAA AATTTGTTGT ACCTCAAGCT 
ATCAAGCCAA AGAAGAATTT CTGTGAACCC AAGAGCAAAA AGATACAGGA GACCAAGGCT 
GAAAAAATTA CTAGAGAGAA TAAGGAGAGG CTATTTGCCA AGGAAGAACA AAAAGAAGAG 
AAAAAATGGA ATGTTATGTC CTTTTCTATT GAGGAGGAAA TGAAAGAGAA TTTAAACTCT 
GGAATAAAGA ACCTAGAAGA GTTTTTGAAA TCATGTAAAA GTAGCTCAGT GAAATTGCGA 
GTTGAAATGG TGGGGATTAC TGCCTGCTTG AGAGCGTGGA AAGAACATTG CCGTGGTGAA 
GGTGAAACCA CAAAAGATTT AAGTATAGCT GTTCAAATGA TGAAAAGGAT CTACTCCGTG 
ACGGAAAAAT ACCCAGAACT TTTGCAAGAA GCAGATTGGC AACTCATAGC CAGATGCCTT 
AGGTATTTAG GATTTGAGGA GTTGGCAAGT TCTTTGCATC CAACCCAGGT CACAGGAAAT 
GACGTAAAGG AGAAGAAAAA GAACAAATAC TCAGTTGGCA TGGGGCCAGC TCGGTTTCAG 
CTGCAGTACA TGGGCCACTA TTTGGTACGA GATGAGAGGA AAGATCCAGA CCCCAGAGTC 
CAGGACTTTA TTCCTGACAC CTGGCAGCGA GAGCTCCTGG ACGTTGTGGA CAAGAACGAG 
TCTGCGGTCA TAGTGGCCCC CACGTCCTCA GGGAAGACCT ACGCCTCCTA CTACTGCATG 
GAGAAGGTGC TGCGGGAGGG TGACGACGGG GTGGTCGTGT ACGTGGCCCC CACCAAGGCC 
CTTGTCAATC AGGTGGCAGC AACTGTTGAG AATCGTTTTA ACAAAAATCT GCCAGACGGT 
GAAACTCTGT GTGGTGTTTT TACACGGGAC TATCGTCATG AGGCTCTAAA CTCTCAGGTA 
CTTATCACAG TGCCTGCCTG CTTTGAAATT CTGCTGCTAG CTCCGCATCG CCAGGCGTGG 
GTGAAAAGGA TCAGATACGT TATATTTGAT GAGGTCCACT GTCTTGGTGG AGAAATTGGA 
GCAGAAGTCT GGGAGCATCT CCTTGTCATG ATCCGCTGTC CCTTCCTGGC CCTTTCTGCT 
ACCATCAGCA ACCCGGAGCA TCTCACCGAG TGGCTACGTT CCATAAAACA GTACTGGAAA 
CAAACAGACA GTACGATGGG AAAAAATATT GCTTCTTCAA GAAATGCCGG CTGCCGTGCC 
AGTTCTCGCC AAGACTACTT ACTGATGAAG CAGTCGTATA AAGTGAGACT TGTGCTCTAT 
GCAGAGAGAT ACAATGATTT AGAGAAGCAC ATATGTTCAG TAAACAATCA TGACATTTCC 
TTTGACCATT TTCACCCGTG CGCTGCGCTG ACCACGGACC ATATTGAAAA GTTTGGATTC 
CCTTCTGATC TTACCCTTTC ACCGCGAGAA ATGATCCAGC TTTATGATGC CATGTTTGAA 
TTCTGGGAAA GTTGGCCCAG GGCTCAGGAG TTGTGTCCGG AGGAATTCGT TCATTTTAAG 
AATAAAATAG TCATTCAAAA GATGGATGCT AGAAAGTATG AAGAGAGCTT AAAGGCAGAA 
CTGACAGGCT GGGTGAAGAG CGGTCACGTC AAGGAGGCCA GAATGGTCCT GAGGAATCTT 
CGTCCTCCTT CGGCTGTTAG CTCAGAAAAC ATGGCAGTTA TGTTTCCACG TCTCGTTGAA 
AAGCTAAGGC AAATGGAGAA GTTACCTGCA CTGTTTTTTT TATTTAAATT AGGGGCCGTA 
GAGAGAAGCG CTGAAAGTGT GAAAACGTTC CTAGAGATAA AGCAACAGAA CAGAAGGCCC 
CCCAAGGCTG ATAAGGAGGC CTATGACCTG GAGAACAAAC TGCGAAAAGT TAGAAAATCC 
CTCGAGGCAC AGAAGACAGT AGATAAAAGA AGCCAGAAAA ATCCCAGAAA AATGGATCAA 
AGCCTAATCC ATGAAGCTGA ACATGATAGT CTCCTCAGGG TTCTTGAGAA GAACTTGGAG 
ATCCCTCAGG ACTGCACATA TGCTGATCAG AAGGCCATAG ATGCTGAGAC TTTGCAGAGA 
GTGTATGACC GAGTGAAATT CTCAAGGAAA GGCGCCAAAC TGAAGGCTTT GGCAGCAAGG 
GGCATCGGAT ATCATCACAG CTCTTTGAGC GCCAAAGAAA AGCAGTTAGT TGAGATTCTT 
TTCAGGAAGG GATTTATTAG GGTGGTGACC GCTACAGGGA CACTTGCTTT AGGAATCAAC 
ATGCCTTGTA AATCTGTGGT TTTTGCTCAA AACTCAGTCT ATCTGGATGC TTTAAACTAC 
AGACAGATGT CTGGACGGGC CGGACGACGA GGTCAAGACC TGGGGGGAGA TGTGTATTTC 
TTTGATATTC CACTCCCTAA AATCGAAAAG CTCATCAAAT CCAACGTCCC TGAACTGAGA 
GGGCAGTTCC CTCTCAGCAT ATCCCTGATC CTGAGACTCA TGTGCCTGGC TTCCAAGGGA 
GATGACCCCG AGGACGCTAG GGCAAAGGTG CTGTCCGTGC TCAAGCACTC ACTGCTCTCC 
TTCAAGCAGC CCCGGGTGCT GGAGATGCTG AAACTGTACT TTCTGTTCTC CCTGCAATTC 
CTGGTCAAGG AGGGCTATTT AGATCAAGAA GGTAATCCTA CAGGCTTTGC TGGACTTGTG 
TCACATTTGC ATTATCACGA GCCTTCTAAT CTTGTTTTCG TCGGTTTTCT TGTAAGAGGC 
CTTTTCCATA ATCTCTGTCA GCCGACCAGC AAAGGCTCAA AACATTTTTC TCAAGATGTT 
ATGGAAAAGC TAGTGTTAGT ATTGGCAAAT CTGTTTGGAA GAAAATATCT TCCAGCACAG 
TTCCAAGATG CTACGCTCAA GTTTTATCAA TCCAAGGTGT TTCTGGAGGA TCTCCCTGAG 
GACTTCAAGG CTGCTCTAGT TGAGTATAAC ACAAATGTGA CAAAGGACTT TGCCTCTTTC 
CTACTGATTG TTTCCAGACT GGCTGATATG AAACAAGAAC ATCAGCTCCC ATTGTCAAAA 
ATAGATTTCT CAGGTGAAGA GTGTGAAGAC TCCCAACTTG TGTCTCACTT GATGAGCTGC 
AAGGAAGGAA GAGTAGCCAT TTCTCCATTT GCCTGTCTCT CTGGGAACTC TGATGGAAAT 
TTGCTTCATC TAGAAACTTC TGACCATGTC ACTCTGCGCA CCATTGGCAT TGATAGCACT 
CGGGCTCCTG TGCTGTGGCC ACAGGGATTT GATCACCAAG GAAGAAGAAT GCAGCTCAAT 
GCTTATGCAC TCGATTTCTA TAGACACGGT TCCTTGACAG GATTGGTCCA GGACAACAGG 
ATGAATGAAG GAGCTGCTTA TCAGTTGTTG AAAGATTTTG GGCTCACCAT TCAATCTATC 
AGTGGGATTT ACTTAGCTCT GCCTCCCTGG GGAAAGCCCT TCTTAAGATA CCGACTCTCA 
GTAGAATGGG TGAGTTTTAT GTGTATCTAT ATTTCCATTT ACAGTGTTTC TTTGCGTGAA 
CTATGTGAAA ATGAAGAGGA CAACGTGGTC TTAGCATTTG AACAACTGAG TGAAACCTTT 
TTTGAAAAAT TTAACAAGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX60 ( XM_028494282.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_028350083.1 Physeter catodon DExD/H-box helicase 60 (LOC102974719), transcript variant X2, mRNA.

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CloneID	OPh331884
Clone ID Related Accession (Same CDS sequence)	XM_028494282.1
Accession Version	XM_028494282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	probable ATP-dependent RNA helicase DDX60 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041222.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTAG AAAAGTACCT GATGCTTTTA GAGAAAATGT CACAGTTGAT TTTGGATCAA 
ATGGCAAAAG CTGATTATGA CAGTCTGTTC AATGATTTTG TTGAGTCTGA GTTTTTTCTG 
ATTGATGGAG ACTCACTGCT GATGACGTAC GTCTGTGAGA AATCCTTGCG GCCTGAACAG 
AGCCTGCACT TCTTCTACCT GGTTGAACAC TACCTTGTGG ACCTAATTGG TAAAGGAGGA 
CAGTTTGCCA TCGTTTTCTT CAAGGATGCT GAGCATGCAT ACAACAACAT CCCTGAATTT 
CTTCCTCTGA GAATGGCATT AATTCTCCAC CTTCAGCATA ATACCAACAT TGATGTTCGA 
ACAGACTTTT CTGGGTGCTT CTCACAAGAG TGGAAAACTT TCTTGGAAGA CCGTTACCCA 
TATTTTCTGA TCGTTTCCGA TGAAAGCTTG AGCCATCTAC AAGTATACCT TTTCAACTTT 
TTAATCATTC AGTCCTGGGC AATGAAGGTC AATGCCGTGC TCTTCTCGGG CCAAGCATCC 
GATGTCCTTC GACTTTATGC GTACTTTATG CCGAGCAAGT ACGAAAACCA GATGTTTTTC 
CAGAAGAATG AAAAGAACAT TGAGAATGCT TATAAAACCC TGATTAAAGA ATTGGAGGAA 
TGTAGAGTTT TAGCATTAGG ACATCTTTTT GGAAATTTAA AATGGAAAAA TATGCTGGAA 
GAGGCATGTG AAACTATTTC TCTGCTTAAG CAACTGTGGC CGGAAGGATC TGACATTCGG 
CAAGTTCTTT GTGTTACTTC ATGCTCCTTG TCTTTGAGTT TATACCATCA CTTTTTAGAA 
AACAGATCGA AGACCACCGA TGCTCAGAAA GCTAACATCC AGCAGCAGAT GGAGAGCGAC 
TGTCCCACCC TGGAGGGGGT GACAGACTGG TGTAGACTGC ACTGTCTCAG CGTGGCCTTT 
CTCCTCCACT TACCTCTTTC CCAGAGAGCA TGTACCAGAT TCATCACTTC CCACTGGATT 
AAGGACATTC AGACTTTCTT AACAGTGAAA AAGTGGTGTG AATACTTCAT CCTAAGTAAT 
ATAGACGCTT TTGAATTTTG GAATCTGAAT TTAACTCACC TTTCCGACTT AAGTGATGAG 
CTTTTATTGA AGAATATAGC ATTTTACTAT GAAAATGAAA GCGTTCAAGG GCTACATTTG 
AATTTGGGAA ATGTCATTAT GAAAGATTAT GAACATCTCT GGGATACTGT ATCAAAGTTG 
GTCACAAAGT TTGATGTTGG AAAGCCATTT CCTCTGAGAA CAAAACAATT TCCTTTTCTT 
GAAAAGGATC CATCACCAAT CAGAGATACC TCCAAGGAAA AGATGGCTAA TTTGGGTTTT 
ATTCCAGTGT CATGTCAGGT GGTTGATAAG TTTGCTGGGG ACATTTTGAA AGATTTGCCT 
TTCCTAACGA GCGATGATCC TATTGTAACT TCACTGGTCA AGCAGAAAGA ATTTGATGAG 
CTTGTGCACT GGCATTCTCA CAGACCCCTC AGTGATGATT ATGACAGGAC AAAGGGCCAC 
TTTGACGAAA AATCTAGAGA TCCTCGTCAT CTTAGATCTG TGCAAATGTA TCATCGTTTT 
CAGCGATTTT ATGGGAAGTC ATTAGAATCA GTCTCTTCAA AATTTGTTGT ACCTCAAGCT 
ATCAAGCCAA AGAAGAATTT CTGTGAACCC AAGAGCAAAA AGATACAGGA GACCAAGGCT 
GAAAAAATTA CTAGAGAGAA TAAGGAGAGG CTATTTGCCA AGGAAGAACA AAAAGAAGAG 
AAAAAATGGA ATGTTATGTC CTTTTCTATT GAGGAGGAAA TGAAAGAGAA TTTAAACTCT 
GGAATAAAGA ACCTAGAAGA GTTTTTGAAA TCATGTAAAA GTAGCTCAGT GAAATTGCGA 
GTTGAAATGG TGGGGATTAC TGCCTGCTTG AGAGCGTGGA AAGAACATTG CCGTGGTGAA 
GGTGAAACCA CAAAAGATTT AAGTATAGCT GTTCAAATGA TGAAAAGGAT CTACTCCGTG 
ACGGAAAAAT ACCCAGAACT TTTGCAAGAA GCAGATTGGC AACTCATAGC CAGATGCCTT 
AGGTATTTAG GATTTGAGGA GTTGGCAAGT TCTTTGCATC CAACCCAGGT CACAGGAAAT 
GACGTAAAGG AGAAGAAAAA GAACAAATAC TCAGTTGGCA TGGGGCCAGC TCGGTTTCAG 
CTGCAGTACA TGGGCCACTA TTTGGTACGA GATGAGAGGA AAGATCCAGA CCCCAGAGTC 
CAGGACTTTA TTCCTGACAC CTGGCAGCGA GAGCTCCTGG ACGTTGTGGA CAAGAACGAG 
TCTGCGGTCA TAGTGGCCCC CACGTCCTCA GGGAAGACCT ACGCCTCCTA CTACTGCATG 
GAGAAGGTGC TGCGGGAGGG TGACGACGGG GTGGTCGTGT ACGTGGCCCC CACCAAGGCC 
CTTGTCAATC AGGTGGCAGC AACTGTTGAG AATCGTTTTA ACAAAAATCT GCCAGACGGT 
GAAACTCTGT GTGGTGTTTT TACACGGGAC TATCGTCATG AGGCTCTAAA CTCTCAGGTA 
CTTATCACAG TGCCTGCCTG CTTTGAAATT CTGCTGCTAG CTCCGCATCG CCAGGCGTGG 
GTGAAAAGGA TCAGATACGT TATATTTGAT GAGGTCCACT GTCTTGGTGG AGAAATTGGA 
GCAGAAGTCT GGGAGCATCT CCTTGTCATG ATCCGCTGTC CCTTCCTGGC CCTTTCTGCT 
ACCATCAGCA ACCCGGAGCA TCTCACCGAG TGGCTACGTT CCATAAAACA GTACTGGAAA 
CAAACAGACA GTACGATGGG AAAAAATATT GCTTCTTCAA GAAATGCCGG CTGCCGTGCC 
AGTTCTCGCC AAGACTACTT ACTGATGAAG CAGTCGTATA AAGTGAGACT TGTGCTCTAT 
GCAGAGAGAT ACAATGATTT AGAGAAGCAC ATATGTTCAG TAAACAATCA TGACATTTCC 
TTTGACCATT TTCACCCGTG CGCTGCGCTG ACCACGGACC ATATTGAAAA GTTTGGATTC 
CCTTCTGATC TTACCCTTTC ACCGCGAGAA ATGATCCAGC TTTATGATGC CATGTTTGAA 
TTCTGGGAAA GTTGGCCCAG GGCTCAGGAG TTGTGTCCGG AGGAATTCGT TCATTTTAAG 
AATAAAATAG TCATTCAAAA GATGGATGCT AGAAAGTATG AAGAGAGCTT AAAGGCAGAA 
CTGACAGGCT GGGTGAAGAG CGGTCACGTC AAGGAGGCCA GAATGGTCCT GAGGAATCTT 
CGTCCTCCTT CGGCTGTTAG CTCAGAAAAC ATGGCAGTTA TGTTTCCACG TCTCGTTGAA 
AAGCTAAGGC AAATGGAGAA GTTACCTGCA CTGTTTTTTT TATTTAAATT AGGGGCCGTA 
GAGAGAAGCG CTGAAAGTGT GAAAACGTTC CTAGAGATAA AGCAACAGAA CAGAAGGCCC 
CCCAAGGCTG ATAAGGAGGC CTATGACCTG GAGAACAAAC TGCGAAAAGT TAGAAAATCC 
CTCGAGGCAC AGAAGACAGT AGATAAAAGA AGCCAGAAAA ATCCCAGAAA AATGGATCAA 
AGCCTAATCC ATGAAGCTGA ACATGATAGT CTCCTCAGGG TTCTTGAGAA GAACTTGGAG 
ATCCCTCAGG ACTGCACATA TGCTGATCAG AAGGCCATAG ATGCTGAGAC TTTGCAGAGA 
GTGTATGACC GAGTGAAATT CTCAAGGAAA GGCGCCAAAC TGAAGGCTTT GGCAGCAAGG 
GGCATCGGAT ATCATCACAG CTCTTTGAGC GCCAAAGAAA AGCAGTTAGT TGAGATTCTT 
TTCAGGAAGG GATTTATTAG GGTGGTGACC GCTACAGGGA CACTTGCTTT AGGAATCAAC 
ATGCCTTGTA AATCTGTGGT TTTTGCTCAA AACTCAGTCT ATCTGGATGC TTTAAACTAC 
AGACAGATGT CTGGACGGGC CGGACGACGA GGTCAAGACC TGGGGGGAGA TGTGTATTTC 
TTTGATATTC CACTCCCTAA AATCGAAAAG CTCATCAAAT CCAACGTCCC TGAACTGAGA 
GGGCAGTTCC CTCTCAGCAT ATCCCTGATC CTGAGACTCA TGTGCCTGGC TTCCAAGGGA 
GATGACCCCG AGGACGCTAG GGCAAAGGTG CTGTCCGTGC TCAAGCACTC ACTGCTCTCC 
TTCAAGCAGC CCCGGGTGCT GGAGATGCTG AAACTGTACT TTCTGTTCTC CCTGCAATTC 
CTGGTCAAGG AGGGCTATTT AGATCAAGAA GGTAATCCTA CAGGCTTTGC TGGACTTGTG 
TCACATTTGC ATTATCACGA GCCTTCTAAT CTTGTTTTCG TCGGTTTTCT TGTAAGAGGC 
CTTTTCCATA ATCTCTGTCA GCCGACCAGC AAAGGCTCAA AACATTTTTC TCAAGATGTT 
ATGGAAAAGC TAGTGTTAGT ATTGGCAAAT CTGTTTGGAA GAAAATATCT TCCAGCACAG 
TTCCAAGATG CTACGCTCAA GTTTTATCAA TCCAAGGTGT TTCTGGAGGA TCTCCCTGAG 
GACTTCAAGG CTGCTCTAGT TGAGTATAAC ACAAATGTGA CAAAGGACTT TGCCTCTTTC 
CTACTGATTG TTTCCAGACT GGCTGATATG AAACAAGAAC ATCAGCTCCC ATTGTCAAAA 
ATAGATTTCT CAGGTGAAGA GTGTGAAGAC TCCCAACTTG TGTCTCACTT GATGAGCTGC 
AAGGAAGGAA GAGTAGCCAT TTCTCCATTT GCCTGTCTCT CTGGGAACTC TGATGGAAAT 
TTGCTTCATC TAGAAACTTC TGACCATGTC ACTCTGCGCA CCATTGGCAT TGATAGCACT 
CGGGCTCCTG TGCTGTGGCC ACAGGGATTT GATCACCAAG GAAGAAGAAT GCAGCTCAAT 
GCTTATGCAC TCGATTTCTA TAGACACGGT TCCTTGACAG GATTGGTCCA GGACAACAGG 
ATGAATGAAG GAGCTGCTTA TCAGTTGTTG AAAGATTTTG GGCTCACCAT TCAATCTATC 
AGTGTTTCTT TGCGTGAACT ATGTGAAAAT GAAGAGGACA ACGTGGTCTT AGCATTTGAA 
CAACTGAGTG AAACCTTTTT TGAAAAATTT AACAAGATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028350083.1
CDS	193..5334
Translation	MGLEKYLMLLEKMSQLILDQMAKADYDSLFNDFVESEFFLIDGDSLLMTYVCEKSLRPEQSLHFFYLVEHYLVDLIGKGGQFAIVFFKDAEHAYNNIPEFLPLRMALILHLQHNTNIDVRTDFSGCFSQEWKTFLEDRYPYFLIVSDESLSHLQVYLFNFLIIQSWAMKVNAVLFSGQASDVLRLYAYFMPSKYENQMFFQKNEKNIENAYKTLIKELEECRVLALGHLFGNLKWKNMLEEACETISLLKQLWPEGSDIRQVLCVTSCSLSLSLYHHFLENRSKTTDAQKANIQQQMESDCPTLEGVTDWCRLHCLSVAFLLHLPLSQRACTRFITSHWIKDIQTFLTVKKWCEYFILSNIDAFEFWNLNLTHLSDLSDELLLKNIAFYYENESVQGLHLNLGNVIMKDYEHLWDTVSKLVTKFDVGKPFPLRTKQFPFLEKDPSPIRDTSKEKMANLGFIPVSCQVVDKFAGDILKDLPFLTSDDPIVTSLVKQKEFDELVHWHSHRPLSDDYDRTKGHFDEKSRDPRHLRSVQMYHRFQRFYGKSLESVSSKFVVPQAIKPKKNFCEPKSKKIQETKAEKITRENKERLFAKEEQKEEKKWNVMSFSIEEEMKENLNSGIKNLEEFLKSCKSSSVKLRVEMVGITACLRAWKEHCRGEGETTKDLSIAVQMMKRIYSVTEKYPELLQEADWQLIARCLRYLGFEELASSLHPTQVTGNDVKEKKKNKYSVGMGPARFQLQYMGHYLVRDERKDPDPRVQDFIPDTWQRELLDVVDKNESAVIVAPTSSGKTYASYYCMEKVLREGDDGVVVYVAPTKALVNQVAATVENRFNKNLPDGETLCGVFTRDYRHEALNSQVLITVPACFEILLLAPHRQAWVKRIRYVIFDEVHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPEHLTEWLRSIKQYWKQTDSTMGKNIASSRNAGCRASSRQDYLLMKQSYKVRLVLYAERYNDLEKHICSVNNHDISFDHFHPCAALTTDHIEKFGFPSDLTLSPREMIQLYDAMFEFWESWPRAQELCPEEFVHFKNKIVIQKMDARKYEESLKAELTGWVKSGHVKEARMVLRNLRPPSAVSSENMAVMFPRLVEKLRQMEKLPALFFLFKLGAVERSAESVKTFLEIKQQNRRPPKADKEAYDLENKLRKVRKSLEAQKTVDKRSQKNPRKMDQSLIHEAEHDSLLRVLEKNLEIPQDCTYADQKAIDAETLQRVYDRVKFSRKGAKLKALAARGIGYHHSSLSAKEKQLVEILFRKGFIRVVTATGTLALGINMPCKSVVFAQNSVYLDALNYRQMSGRAGRRGQDLGGDVYFFDIPLPKIEKLIKSNVPELRGQFPLSISLILRLMCLASKGDDPEDARAKVLSVLKHSLLSFKQPRVLEMLKLYFLFSLQFLVKEGYLDQEGNPTGFAGLVSHLHYHEPSNLVFVGFLVRGLFHNLCQPTSKGSKHFSQDVMEKLVLVLANLFGRKYLPAQFQDATLKFYQSKVFLEDLPEDFKAALVEYNTNVTKDFASFLLIVSRLADMKQEHQLPLSKIDFSGEECEDSQLVSHLMSCKEGRVAISPFACLSGNSDGNLLHLETSDHVTLRTIGIDSTRAPVLWPQGFDHQGRRMQLNAYALDFYRHGSLTGLVQDNRMNEGAAYQLLKDFGLTIQSISVSLRELCENEEDNVVLAFEQLSETFFEKFNKI

Target ORF information:

RefSeq Version	XM_028494282.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon DExD/H-box helicase 60 (LOC102974719), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028494282.1

ORF Insert Sequence:

ATGGGTTTAG AAAAGTACCT GATGCTTTTA GAGAAAATGT CACAGTTGAT TTTGGATCAA 
ATGGCAAAAG CTGATTATGA CAGTCTGTTC AATGATTTTG TTGAGTCTGA GTTTTTTCTG 
ATTGATGGAG ACTCACTGCT GATGACGTAC GTCTGTGAGA AATCCTTGCG GCCTGAACAG 
AGCCTGCACT TCTTCTACCT GGTTGAACAC TACCTTGTGG ACCTAATTGG TAAAGGAGGA 
CAGTTTGCCA TCGTTTTCTT CAAGGATGCT GAGCATGCAT ACAACAACAT CCCTGAATTT 
CTTCCTCTGA GAATGGCATT AATTCTCCAC CTTCAGCATA ATACCAACAT TGATGTTCGA 
ACAGACTTTT CTGGGTGCTT CTCACAAGAG TGGAAAACTT TCTTGGAAGA CCGTTACCCA 
TATTTTCTGA TCGTTTCCGA TGAAAGCTTG AGCCATCTAC AAGTATACCT TTTCAACTTT 
TTAATCATTC AGTCCTGGGC AATGAAGGTC AATGCCGTGC TCTTCTCGGG CCAAGCATCC 
GATGTCCTTC GACTTTATGC GTACTTTATG CCGAGCAAGT ACGAAAACCA GATGTTTTTC 
CAGAAGAATG AAAAGAACAT TGAGAATGCT TATAAAACCC TGATTAAAGA ATTGGAGGAA 
TGTAGAGTTT TAGCATTAGG ACATCTTTTT GGAAATTTAA AATGGAAAAA TATGCTGGAA 
GAGGCATGTG AAACTATTTC TCTGCTTAAG CAACTGTGGC CGGAAGGATC TGACATTCGG 
CAAGTTCTTT GTGTTACTTC ATGCTCCTTG TCTTTGAGTT TATACCATCA CTTTTTAGAA 
AACAGATCGA AGACCACCGA TGCTCAGAAA GCTAACATCC AGCAGCAGAT GGAGAGCGAC 
TGTCCCACCC TGGAGGGGGT GACAGACTGG TGTAGACTGC ACTGTCTCAG CGTGGCCTTT 
CTCCTCCACT TACCTCTTTC CCAGAGAGCA TGTACCAGAT TCATCACTTC CCACTGGATT 
AAGGACATTC AGACTTTCTT AACAGTGAAA AAGTGGTGTG AATACTTCAT CCTAAGTAAT 
ATAGACGCTT TTGAATTTTG GAATCTGAAT TTAACTCACC TTTCCGACTT AAGTGATGAG 
CTTTTATTGA AGAATATAGC ATTTTACTAT GAAAATGAAA GCGTTCAAGG GCTACATTTG 
AATTTGGGAA ATGTCATTAT GAAAGATTAT GAACATCTCT GGGATACTGT ATCAAAGTTG 
GTCACAAAGT TTGATGTTGG AAAGCCATTT CCTCTGAGAA CAAAACAATT TCCTTTTCTT 
GAAAAGGATC CATCACCAAT CAGAGATACC TCCAAGGAAA AGATGGCTAA TTTGGGTTTT 
ATTCCAGTGT CATGTCAGGT GGTTGATAAG TTTGCTGGGG ACATTTTGAA AGATTTGCCT 
TTCCTAACGA GCGATGATCC TATTGTAACT TCACTGGTCA AGCAGAAAGA ATTTGATGAG 
CTTGTGCACT GGCATTCTCA CAGACCCCTC AGTGATGATT ATGACAGGAC AAAGGGCCAC 
TTTGACGAAA AATCTAGAGA TCCTCGTCAT CTTAGATCTG TGCAAATGTA TCATCGTTTT 
CAGCGATTTT ATGGGAAGTC ATTAGAATCA GTCTCTTCAA AATTTGTTGT ACCTCAAGCT 
ATCAAGCCAA AGAAGAATTT CTGTGAACCC AAGAGCAAAA AGATACAGGA GACCAAGGCT 
GAAAAAATTA CTAGAGAGAA TAAGGAGAGG CTATTTGCCA AGGAAGAACA AAAAGAAGAG 
AAAAAATGGA ATGTTATGTC CTTTTCTATT GAGGAGGAAA TGAAAGAGAA TTTAAACTCT 
GGAATAAAGA ACCTAGAAGA GTTTTTGAAA TCATGTAAAA GTAGCTCAGT GAAATTGCGA 
GTTGAAATGG TGGGGATTAC TGCCTGCTTG AGAGCGTGGA AAGAACATTG CCGTGGTGAA 
GGTGAAACCA CAAAAGATTT AAGTATAGCT GTTCAAATGA TGAAAAGGAT CTACTCCGTG 
ACGGAAAAAT ACCCAGAACT TTTGCAAGAA GCAGATTGGC AACTCATAGC CAGATGCCTT 
AGGTATTTAG GATTTGAGGA GTTGGCAAGT TCTTTGCATC CAACCCAGGT CACAGGAAAT 
GACGTAAAGG AGAAGAAAAA GAACAAATAC TCAGTTGGCA TGGGGCCAGC TCGGTTTCAG 
CTGCAGTACA TGGGCCACTA TTTGGTACGA GATGAGAGGA AAGATCCAGA CCCCAGAGTC 
CAGGACTTTA TTCCTGACAC CTGGCAGCGA GAGCTCCTGG ACGTTGTGGA CAAGAACGAG 
TCTGCGGTCA TAGTGGCCCC CACGTCCTCA GGGAAGACCT ACGCCTCCTA CTACTGCATG 
GAGAAGGTGC TGCGGGAGGG TGACGACGGG GTGGTCGTGT ACGTGGCCCC CACCAAGGCC 
CTTGTCAATC AGGTGGCAGC AACTGTTGAG AATCGTTTTA ACAAAAATCT GCCAGACGGT 
GAAACTCTGT GTGGTGTTTT TACACGGGAC TATCGTCATG AGGCTCTAAA CTCTCAGGTA 
CTTATCACAG TGCCTGCCTG CTTTGAAATT CTGCTGCTAG CTCCGCATCG CCAGGCGTGG 
GTGAAAAGGA TCAGATACGT TATATTTGAT GAGGTCCACT GTCTTGGTGG AGAAATTGGA 
GCAGAAGTCT GGGAGCATCT CCTTGTCATG ATCCGCTGTC CCTTCCTGGC CCTTTCTGCT 
ACCATCAGCA ACCCGGAGCA TCTCACCGAG TGGCTACGTT CCATAAAACA GTACTGGAAA 
CAAACAGACA GTACGATGGG AAAAAATATT GCTTCTTCAA GAAATGCCGG CTGCCGTGCC 
AGTTCTCGCC AAGACTACTT ACTGATGAAG CAGTCGTATA AAGTGAGACT TGTGCTCTAT 
GCAGAGAGAT ACAATGATTT AGAGAAGCAC ATATGTTCAG TAAACAATCA TGACATTTCC 
TTTGACCATT TTCACCCGTG CGCTGCGCTG ACCACGGACC ATATTGAAAA GTTTGGATTC 
CCTTCTGATC TTACCCTTTC ACCGCGAGAA ATGATCCAGC TTTATGATGC CATGTTTGAA 
TTCTGGGAAA GTTGGCCCAG GGCTCAGGAG TTGTGTCCGG AGGAATTCGT TCATTTTAAG 
AATAAAATAG TCATTCAAAA GATGGATGCT AGAAAGTATG AAGAGAGCTT AAAGGCAGAA 
CTGACAGGCT GGGTGAAGAG CGGTCACGTC AAGGAGGCCA GAATGGTCCT GAGGAATCTT 
CGTCCTCCTT CGGCTGTTAG CTCAGAAAAC ATGGCAGTTA TGTTTCCACG TCTCGTTGAA 
AAGCTAAGGC AAATGGAGAA GTTACCTGCA CTGTTTTTTT TATTTAAATT AGGGGCCGTA 
GAGAGAAGCG CTGAAAGTGT GAAAACGTTC CTAGAGATAA AGCAACAGAA CAGAAGGCCC 
CCCAAGGCTG ATAAGGAGGC CTATGACCTG GAGAACAAAC TGCGAAAAGT TAGAAAATCC 
CTCGAGGCAC AGAAGACAGT AGATAAAAGA AGCCAGAAAA ATCCCAGAAA AATGGATCAA 
AGCCTAATCC ATGAAGCTGA ACATGATAGT CTCCTCAGGG TTCTTGAGAA GAACTTGGAG 
ATCCCTCAGG ACTGCACATA TGCTGATCAG AAGGCCATAG ATGCTGAGAC TTTGCAGAGA 
GTGTATGACC GAGTGAAATT CTCAAGGAAA GGCGCCAAAC TGAAGGCTTT GGCAGCAAGG 
GGCATCGGAT ATCATCACAG CTCTTTGAGC GCCAAAGAAA AGCAGTTAGT TGAGATTCTT 
TTCAGGAAGG GATTTATTAG GGTGGTGACC GCTACAGGGA CACTTGCTTT AGGAATCAAC 
ATGCCTTGTA AATCTGTGGT TTTTGCTCAA AACTCAGTCT ATCTGGATGC TTTAAACTAC 
AGACAGATGT CTGGACGGGC CGGACGACGA GGTCAAGACC TGGGGGGAGA TGTGTATTTC 
TTTGATATTC CACTCCCTAA AATCGAAAAG CTCATCAAAT CCAACGTCCC TGAACTGAGA 
GGGCAGTTCC CTCTCAGCAT ATCCCTGATC CTGAGACTCA TGTGCCTGGC TTCCAAGGGA 
GATGACCCCG AGGACGCTAG GGCAAAGGTG CTGTCCGTGC TCAAGCACTC ACTGCTCTCC 
TTCAAGCAGC CCCGGGTGCT GGAGATGCTG AAACTGTACT TTCTGTTCTC CCTGCAATTC 
CTGGTCAAGG AGGGCTATTT AGATCAAGAA GGTAATCCTA CAGGCTTTGC TGGACTTGTG 
TCACATTTGC ATTATCACGA GCCTTCTAAT CTTGTTTTCG TCGGTTTTCT TGTAAGAGGC 
CTTTTCCATA ATCTCTGTCA GCCGACCAGC AAAGGCTCAA AACATTTTTC TCAAGATGTT 
ATGGAAAAGC TAGTGTTAGT ATTGGCAAAT CTGTTTGGAA GAAAATATCT TCCAGCACAG 
TTCCAAGATG CTACGCTCAA GTTTTATCAA TCCAAGGTGT TTCTGGAGGA TCTCCCTGAG 
GACTTCAAGG CTGCTCTAGT TGAGTATAAC ACAAATGTGA CAAAGGACTT TGCCTCTTTC 
CTACTGATTG TTTCCAGACT GGCTGATATG AAACAAGAAC ATCAGCTCCC ATTGTCAAAA 
ATAGATTTCT CAGGTGAAGA GTGTGAAGAC TCCCAACTTG TGTCTCACTT GATGAGCTGC 
AAGGAAGGAA GAGTAGCCAT TTCTCCATTT GCCTGTCTCT CTGGGAACTC TGATGGAAAT 
TTGCTTCATC TAGAAACTTC TGACCATGTC ACTCTGCGCA CCATTGGCAT TGATAGCACT 
CGGGCTCCTG TGCTGTGGCC ACAGGGATTT GATCACCAAG GAAGAAGAAT GCAGCTCAAT 
GCTTATGCAC TCGATTTCTA TAGACACGGT TCCTTGACAG GATTGGTCCA GGACAACAGG 
ATGAATGAAG GAGCTGCTTA TCAGTTGTTG AAAGATTTTG GGCTCACCAT TCAATCTATC 
AGTGTTTCTT TGCGTGAACT ATGTGAAAAT GAAGAGGACA ACGTGGTCTT AGCATTTGAA 
CAACTGAGTG AAACCTTTTT TGAAAAATTT AACAAGATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DDX60 cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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