Pc20g03820 cDNA ORF clone, Penicillium rubens Wisconsin 54-1255

Gene
Clones
gRNAs

Summary

Gene Symbol	Pc20g03820
Entrez Gene ID	8309987
Full Name	Pc20g03820
Gene Type	protein-coding
Organism	Penicillium rubens Wisconsin 54-1255

mRNA and Protein(s)

mRNA	Protein	Name
XM_002562886.1	XP_002562932.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum.
van den Berg MA, Albang R, Albermann K, Badger JH, Daran JM, Driessen AJ, Garcia-Estrada C, Fedorova ND, Harris DM, Heijne WH, Joardar V, Kiel JA, Kovalchuk A, Martín JF, Nierman WC, Nijland JG, Pronk JT, Roubos JA, van der Klei IJ, van Peij NN, Veenhuis M, von Döhren H, Wagner C, Wortman J, Bovenberg RA
Nature biotechnology26(10)1161-8(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pc20g03820 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pc20g03820 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPg05656	XM_002562886.1 Latest version!	Penicillium chrysogenum Wisconsin 54-1255 hypothetical protein (Pc20g03820) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pc20g03820 ( XM_002562886.1 ) cDNA ORF clone, Penicillium rubens Wisconsin 54-1255 -> XP_002562932.1 Penicillium chrysogenum Wisconsin 54-1255 hypothetical protein (Pc20g03820) mRNA, complete cds.

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CloneID	OPg05656
Clone ID Related Accession (Same CDS sequence)	XM_002562886.1
Accession Version	XM_002562886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1250092800000
Organism	Penicillium rubens Wisconsin 54-1255
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003020080). COMPLETENESS: incomplete on both ends.

ATGGCTGCGG AGGAGTTGTC GGAGGGCGGC ATGGCCGACA TCATCCGTGC CCTGCAACTC 
ATTCACAACC CTGCATCGTC AAACGATATA CGGAAGGAGG CTTCGCAGTT CGTGGAGAAT 
CTGAAAGAGT CTGACGCTGC GGCTCGAAAT GGCTTTCTCT TGGCCTCCCG TATGGAGCAT 
GAGCCTGTTG TACGATACTT CGGTCTTACT CTCCTCGATC ACGTTCTTCG CCAATACAAC 
TTCACCAATC CCGAAGAAGC CAAGAATTTA CGCATGATGA TTTTGCAGCT CGCCGAGAAC 
GTTCGCCCCG AAGACCCTGC CTATTTTCGG AATAAGATCT CCCAGTTGTG GACCGAGGCT 
GCTAAGAAGA CCTGGGGGCT GGATTGGATG GATATGGATG CCAACCTGGT TCAGTTCTGG 
GGTGCATCTC TGGTTCACAA AGAGTTGGTA CTTGCTGTTC TGGAGACTCT TTCCGAGGAT 
GTCTTTTTCC GAGAAGATAC CGCCTCGTCA CTTCGCGGAG GAGAGCTCAA TCGGTCGCTC 
GTCGAGATTT TTACCCCTGC AGGCATAGTG GAGCAGATTA CTGGGGATAA AACTACCGCC 
ACCATTCCAC GCTGTGGACA GGAGGGCTGG CTCGTTCGCA TCTGCGAATT TCTTGACAAC 
TGCGTCCAGA ATGTCTCTAG CTCGGAACAG GCTCGGGATG CGGCCACAAA AGCTCTGGCA 
ACTTTGAGAT CTGCCTTGTC ATGGTCCGTC TATAAAGGCG TTATTGCCTC CCAGGTCGTT 
CCCAGCATTT TCCGAGCTTT GCCTTGTCAA GATGACCAGG TGCTTCTCTC TGCGGTCGAG 
GCACTTCATG CGCTTTATGG AAGGAACACT ATCGGCAGTG AAGACTCGCA GGAGCTAGTC 
TGCCTGATAT TTCAGCCAGT GTACCTTGTT GTTCTCCAAC GGCTTTACGA ATGGTCAATT 
GTCGGCCCGG ACGACGTCGA TGAACCCAAA TATCTTATCT CCAAAAAGCT TTCCGAGATG 
ATTTCATATA TTGCAGGATG CTTGGAAGAT GACAAGTTCT TAGTCTCTAC CATGGACCGT 
TTGAATCTGC CGCCATTCCT GCAATTCATG GTCAACATCA TGCAGCACCA GAGCTTGACG 
GTCTCCATAC CTTGCTTGCA TCTTTGGTCC AGACTGTTAC AAAACTCAAA AATCAGTAAC 
CTGGACTTCG TGGTAGAACA AACTCCTCAA TTTTTGAACA TCTGCACCCA GAGGCTTCTT 
CGTTGGGAGT CTCTCCCGAC TGAATCCGAG GATCCCACGG TACAGTTCTT AGTAGAGGAT 
ATTGATACAG TCCCCGAACG ACATGCATTT GTTGGAAACT ACCGCCGTTA CTGTTCTTCG 
ATCATCGAAA CCATCACCTT CAAGCGGCCC CAAGAAGCGG TGCGGGAAAT CCTGGCCCGG 
GTGGACACCA ACCTCGACAA CTTGTATAAC GGCGTTCAGC CATTTAGCAT GGCAACTTTC 
AGCAAGTCGT CGATTCCTCT CATGCGAGCG GATGCTCAAT TCGCAGTGGT AGAGGCTGTC 
ATCAAGGGCT ACAACAAGTG GATTTCGGCG CATGGAAAAG CACCCCAACG TGATGAGTCT 
AAACGAATGG AATTAGAACA TGCGGTGGAA AACTGGGCTT CAACTCTAAT GCAAAAGTCC 
TTCGAGGATC CGGTTTTGAA ACAAAGGGTC ATCAAATTAG TTGTCGACAT CTCCTCAAAG 
GCCTTGGACA ATCATCAAGG ATTTGCACTC AAAGTTCTGG AGCACATTCT CATGTCACGT 
CTACCAGATC GGTCTGAATA CCCATTGTAT TCCGAGGCTG TCAAGGAACT TCATGGACTG 
GCAAGCTCCG AACTTCGTCG GTTAGCTATG CGTTATGCCG ATTACTTCTA TACTTTCTAT 
GATCTTCTTG AGCCAAAGAT CAAAGAGATC ACACTGGCTA ACCGCATCGA TGACAAGTTG 
CAAATGGAAC TGACTTCTAT CTTGCTCATT ATTATGCAAC GTGCAATCAA TGCAGACCCT 
TATCTGCGTC AAAATCGGCT CGCATCATTC CTTCAACCCA TCCAAGAGGC GTGGCAAGAT 
GACCAATTCC GTCACAGCAG CTCCACTTTC CCCGGTTTTT GCAATATGCT CGGATTGGAA 
AATGTTGGTC CATACATGCA ATCCAAAGAA GCACAGAAAC TGGCAGACTG GTCTGAGGTT 
GCCTTGGACA ACGAAGGAAG GTTAGTCCAG GAAGAGATGA CCAGAAAGTT CCAGCTATTG 
CCATTGCGTG GCACCAAAAC TATGTTGGCT GTTTCTACAG ACCGAGTCAA AAAGTCCTCG 
CCAGCCTATG CGACCGCCTG TCAGATGTGG CACGAATTGA TCCCTATCAT TCTTCCCACT 
TTGCTGCAGC TTGTCAGAAA CGCGCATGCC TTCCATAACC CTGCCAATTG GAACATGGTC 
GAAGGAATGC AACCAGTAGT CGAACGTATC CTCACGGATC GATTTTGGCA GGCTGGTATT 
TCTATCGGCA GCCGAGATGA GTTCTATGCC AGAATCACAT CGTCGAAGTC TACACTTGAA 
GGGTTTGCAT CATCAGTCCG AGGAAAAGTC AGAGCTGTCC GCGAGGCATG TTACTCCATG 
CTATTCAGCA TGAGCAGGAT GCGAGAGCAC TTTTATGGAT TCGCCGAACT CCCAGGACCC 
CTATCCGAAG CGTTATTCGT TGACTCACCG CATCTCTCCT CCCATCAGTT CTCGGTTCTC 
CTCAACATCT CGCGTTGTCT GATTGACGAC TGCCCTGTGC AGTTCCGCAG CCAATTCTTG 
CCCCCCATGT TGTCAACTCT ATTCACCAAC ATTGACCGCA AAGTCACCAC AGAATGGGAA 
ATCATTGAAC AACGCAGGAA CGGAATTTCG GATGGAGATC TCACGACGGA GATGAAGTCC 
GAAAGTGTCC TTCGCCAGTT GACTTACTCT GCTGTGATCA TGGTAGCCAG TCTATTCGAT 
CCGCAGAGAG GTGACCCAGA TGGGACTGAA TCGGACCCCA CCGCACCCCA GCCGACTCCG 
AACCTCTCTG ATTCTATCCG CCATTTTGTC CTTTCGTCCC CCCAAATCTT TGAGCCAGTC 
ATGCTTTTCT GTACGCACGC TCTTCGTATG CGCGATACAC GCAGCGGCAG CATTATCACT 
CGCGTAATTC GGTCAATTCT GCAAGACTTC GCCCCCACGA ATGATACCCC GGACACCCCA 
ACCATCGCGA CCATCCGCGA ATTCATATGT ACTGATGTCC TCACAGCATG CATTTCATCC 
GTACATGAGT CATACTTTGT TGATATGCAA AAGGACCTTG CCCAGCTAAT TGCATCGATT 
TGGTGTCTCT ATGGCTTCTG TAGCGAGACT CCTCGTGCTG TTTTCCTGAG CTTGCCAGGA 
ATTAGCGCAG AGAAAGTGGC CAGTACTGAG TCTGCACTTC ATCAAACTAC CTCACCAAGA 
CAGCAGCGCG CATTAGTCTT GGAGCTTCTT GAGCCCTTGC GCGGCATCAG TGTTGCCGAG 
CAAGGGAAGA TACTAGGTTC TCGCGAGGAA CGTCGCAAGG CCCGCTCAGC ATTGCAGGAG 
AGATACATGA CCAATGAGAT GGACACCCAG CAGCAGAACC ACCGAGTCGA TATTAACGAT 
GGTCCCGATC TTGGTGGTGT GGCTGATCTA TTTGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002562932.1
CDS	1..3699
Translation	MAAEELSEGGMADIIRALQLIHNPASSNDIRKEASQFVENLKESDAAARNGFLLASRMEHEPVVRYFGLTLLDHVLRQYNFTNPEEAKNLRMMILQLAENVRPEDPAYFRNKISQLWTEAAKKTWGLDWMDMDANLVQFWGASLVHKELVLAVLETLSEDVFFREDTASSLRGGELNRSLVEIFTPAGIVEQITGDKTTATIPRCGQEGWLVRICEFLDNCVQNVSSSEQARDAATKALATLRSALSWSVYKGVIASQVVPSIFRALPCQDDQVLLSAVEALHALYGRNTIGSEDSQELVCLIFQPVYLVVLQRLYEWSIVGPDDVDEPKYLISKKLSEMISYIAGCLEDDKFLVSTMDRLNLPPFLQFMVNIMQHQSLTVSIPCLHLWSRLLQNSKISNLDFVVEQTPQFLNICTQRLLRWESLPTESEDPTVQFLVEDIDTVPERHAFVGNYRRYCSSIIETITFKRPQEAVREILARVDTNLDNLYNGVQPFSMATFSKSSIPLMRADAQFAVVEAVIKGYNKWISAHGKAPQRDESKRMELEHAVENWASTLMQKSFEDPVLKQRVIKLVVDISSKALDNHQGFALKVLEHILMSRLPDRSEYPLYSEAVKELHGLASSELRRLAMRYADYFYTFYDLLEPKIKEITLANRIDDKLQMELTSILLIIMQRAINADPYLRQNRLASFLQPIQEAWQDDQFRHSSSTFPGFCNMLGLENVGPYMQSKEAQKLADWSEVALDNEGRLVQEEMTRKFQLLPLRGTKTMLAVSTDRVKKSSPAYATACQMWHELIPIILPTLLQLVRNAHAFHNPANWNMVEGMQPVVERILTDRFWQAGISIGSRDEFYARITSSKSTLEGFASSVRGKVRAVREACYSMLFSMSRMREHFYGFAELPGPLSEALFVDSPHLSSHQFSVLLNISRCLIDDCPVQFRSQFLPPMLSTLFTNIDRKVTTEWEIIEQRRNGISDGDLTTEMKSESVLRQLTYSAVIMVASLFDPQRGDPDGTESDPTAPQPTPNLSDSIRHFVLSSPQIFEPVMLFCTHALRMRDTRSGSIITRVIRSILQDFAPTNDTPDTPTIATIREFICTDVLTACISSVHESYFVDMQKDLAQLIASIWCLYGFCSETPRAVFLSLPGISAEKVASTESALHQTTSPRQQRALVLELLEPLRGISVAEQGKILGSREERRKARSALQERYMTNEMDTQQQNHRVDINDGPDLGGVADLFG

Target ORF information:

RefSeq Version	XM_002562886.1
Organism	Penicillium rubens Wisconsin 54-1255
Definition	Penicillium chrysogenum Wisconsin 54-1255 hypothetical protein (Pc20g03820) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002562886.1

ORF Insert Sequence:

ATGGCTGCGG AGGAGTTGTC GGAGGGCGGC ATGGCCGACA TCATCCGTGC CCTGCAACTC 
ATTCACAACC CTGCATCGTC AAACGATATA CGGAAGGAGG CTTCGCAGTT CGTGGAGAAT 
CTGAAAGAGT CTGACGCTGC GGCTCGAAAT GGCTTTCTCT TGGCCTCCCG TATGGAGCAT 
GAGCCTGTTG TACGATACTT CGGTCTTACT CTCCTCGATC ACGTTCTTCG CCAATACAAC 
TTCACCAATC CCGAAGAAGC CAAGAATTTA CGCATGATGA TTTTGCAGCT CGCCGAGAAC 
GTTCGCCCCG AAGACCCTGC CTATTTTCGG AATAAGATCT CCCAGTTGTG GACCGAGGCT 
GCTAAGAAGA CCTGGGGGCT GGATTGGATG GATATGGATG CCAACCTGGT TCAGTTCTGG 
GGTGCATCTC TGGTTCACAA AGAGTTGGTA CTTGCTGTTC TGGAGACTCT TTCCGAGGAT 
GTCTTTTTCC GAGAAGATAC CGCCTCGTCA CTTCGCGGAG GAGAGCTCAA TCGGTCGCTC 
GTCGAGATTT TTACCCCTGC AGGCATAGTG GAGCAGATTA CTGGGGATAA AACTACCGCC 
ACCATTCCAC GCTGTGGACA GGAGGGCTGG CTCGTTCGCA TCTGCGAATT TCTTGACAAC 
TGCGTCCAGA ATGTCTCTAG CTCGGAACAG GCTCGGGATG CGGCCACAAA AGCTCTGGCA 
ACTTTGAGAT CTGCCTTGTC ATGGTCCGTC TATAAAGGCG TTATTGCCTC CCAGGTCGTT 
CCCAGCATTT TCCGAGCTTT GCCTTGTCAA GATGACCAGG TGCTTCTCTC TGCGGTCGAG 
GCACTTCATG CGCTTTATGG AAGGAACACT ATCGGCAGTG AAGACTCGCA GGAGCTAGTC 
TGCCTGATAT TTCAGCCAGT GTACCTTGTT GTTCTCCAAC GGCTTTACGA ATGGTCAATT 
GTCGGCCCGG ACGACGTCGA TGAACCCAAA TATCTTATCT CCAAAAAGCT TTCCGAGATG 
ATTTCATATA TTGCAGGATG CTTGGAAGAT GACAAGTTCT TAGTCTCTAC CATGGACCGT 
TTGAATCTGC CGCCATTCCT GCAATTCATG GTCAACATCA TGCAGCACCA GAGCTTGACG 
GTCTCCATAC CTTGCTTGCA TCTTTGGTCC AGACTGTTAC AAAACTCAAA AATCAGTAAC 
CTGGACTTCG TGGTAGAACA AACTCCTCAA TTTTTGAACA TCTGCACCCA GAGGCTTCTT 
CGTTGGGAGT CTCTCCCGAC TGAATCCGAG GATCCCACGG TACAGTTCTT AGTAGAGGAT 
ATTGATACAG TCCCCGAACG ACATGCATTT GTTGGAAACT ACCGCCGTTA CTGTTCTTCG 
ATCATCGAAA CCATCACCTT CAAGCGGCCC CAAGAAGCGG TGCGGGAAAT CCTGGCCCGG 
GTGGACACCA ACCTCGACAA CTTGTATAAC GGCGTTCAGC CATTTAGCAT GGCAACTTTC 
AGCAAGTCGT CGATTCCTCT CATGCGAGCG GATGCTCAAT TCGCAGTGGT AGAGGCTGTC 
ATCAAGGGCT ACAACAAGTG GATTTCGGCG CATGGAAAAG CACCCCAACG TGATGAGTCT 
AAACGAATGG AATTAGAACA TGCGGTGGAA AACTGGGCTT CAACTCTAAT GCAAAAGTCC 
TTCGAGGATC CGGTTTTGAA ACAAAGGGTC ATCAAATTAG TTGTCGACAT CTCCTCAAAG 
GCCTTGGACA ATCATCAAGG ATTTGCACTC AAAGTTCTGG AGCACATTCT CATGTCACGT 
CTACCAGATC GGTCTGAATA CCCATTGTAT TCCGAGGCTG TCAAGGAACT TCATGGACTG 
GCAAGCTCCG AACTTCGTCG GTTAGCTATG CGTTATGCCG ATTACTTCTA TACTTTCTAT 
GATCTTCTTG AGCCAAAGAT CAAAGAGATC ACACTGGCTA ACCGCATCGA TGACAAGTTG 
CAAATGGAAC TGACTTCTAT CTTGCTCATT ATTATGCAAC GTGCAATCAA TGCAGACCCT 
TATCTGCGTC AAAATCGGCT CGCATCATTC CTTCAACCCA TCCAAGAGGC GTGGCAAGAT 
GACCAATTCC GTCACAGCAG CTCCACTTTC CCCGGTTTTT GCAATATGCT CGGATTGGAA 
AATGTTGGTC CATACATGCA ATCCAAAGAA GCACAGAAAC TGGCAGACTG GTCTGAGGTT 
GCCTTGGACA ACGAAGGAAG GTTAGTCCAG GAAGAGATGA CCAGAAAGTT CCAGCTATTG 
CCATTGCGTG GCACCAAAAC TATGTTGGCT GTTTCTACAG ACCGAGTCAA AAAGTCCTCG 
CCAGCCTATG CGACCGCCTG TCAGATGTGG CACGAATTGA TCCCTATCAT TCTTCCCACT 
TTGCTGCAGC TTGTCAGAAA CGCGCATGCC TTCCATAACC CTGCCAATTG GAACATGGTC 
GAAGGAATGC AACCAGTAGT CGAACGTATC CTCACGGATC GATTTTGGCA GGCTGGTATT 
TCTATCGGCA GCCGAGATGA GTTCTATGCC AGAATCACAT CGTCGAAGTC TACACTTGAA 
GGGTTTGCAT CATCAGTCCG AGGAAAAGTC AGAGCTGTCC GCGAGGCATG TTACTCCATG 
CTATTCAGCA TGAGCAGGAT GCGAGAGCAC TTTTATGGAT TCGCCGAACT CCCAGGACCC 
CTATCCGAAG CGTTATTCGT TGACTCACCG CATCTCTCCT CCCATCAGTT CTCGGTTCTC 
CTCAACATCT CGCGTTGTCT GATTGACGAC TGCCCTGTGC AGTTCCGCAG CCAATTCTTG 
CCCCCCATGT TGTCAACTCT ATTCACCAAC ATTGACCGCA AAGTCACCAC AGAATGGGAA 
ATCATTGAAC AACGCAGGAA CGGAATTTCG GATGGAGATC TCACGACGGA GATGAAGTCC 
GAAAGTGTCC TTCGCCAGTT GACTTACTCT GCTGTGATCA TGGTAGCCAG TCTATTCGAT 
CCGCAGAGAG GTGACCCAGA TGGGACTGAA TCGGACCCCA CCGCACCCCA GCCGACTCCG 
AACCTCTCTG ATTCTATCCG CCATTTTGTC CTTTCGTCCC CCCAAATCTT TGAGCCAGTC 
ATGCTTTTCT GTACGCACGC TCTTCGTATG CGCGATACAC GCAGCGGCAG CATTATCACT 
CGCGTAATTC GGTCAATTCT GCAAGACTTC GCCCCCACGA ATGATACCCC GGACACCCCA 
ACCATCGCGA CCATCCGCGA ATTCATATGT ACTGATGTCC TCACAGCATG CATTTCATCC 
GTACATGAGT CATACTTTGT TGATATGCAA AAGGACCTTG CCCAGCTAAT TGCATCGATT 
TGGTGTCTCT ATGGCTTCTG TAGCGAGACT CCTCGTGCTG TTTTCCTGAG CTTGCCAGGA 
ATTAGCGCAG AGAAAGTGGC CAGTACTGAG TCTGCACTTC ATCAAACTAC CTCACCAAGA 
CAGCAGCGCG CATTAGTCTT GGAGCTTCTT GAGCCCTTGC GCGGCATCAG TGTTGCCGAG 
CAAGGGAAGA TACTAGGTTC TCGCGAGGAA CGTCGCAAGG CCCGCTCAGC ATTGCAGGAG 
AGATACATGA CCAATGAGAT GGACACCCAG CAGCAGAACC ACCGAGTCGA TATTAACGAT 
GGTCCCGATC TTGGTGGTGT GGCTGATCTA TTTGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum.
Nature biotechnology26(10)1161-8(2008 Oct)
van den Berg MA,Albang R,Albermann K,Badger JH,Daran JM,Driessen AJ,Garcia-Estrada C,Fedorova ND,Harris DM,Heijne WH,Joardar V,Kiel JA,Kovalchuk A,Martín JF,Nierman WC,Nijland JG,Pronk JT,Roubos JA,van der Klei IJ,van Peij NN,Veenhuis M,von Döhren H,Wagner C,Wortman J,Bovenberg RA

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Pc20g03820 cDNA ORF clone, Penicillium rubens Wisconsin 54-1255

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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