RASGRF2 cDNA ORF clone, Pan paniscus(pygmy chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGRF2
Entrez Gene ID	100971503
Full Name	Ras protein specific guanine nucleotide releasing factor 2
Gene Type	protein-coding
Organism	Pan paniscus(pygmy chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003822263.2	XP_003822311.1	ras-specific guanine nucleotide-releasing factor 2 isoform X1
XM_014344865.1	XP_014200351.1	ras-specific guanine nucleotide-releasing factor 2 isoform X2
XM_024928740.1	XP_024784508.1	ras-specific guanine nucleotide-releasing factor 2 isoform X2
XM_014344865.2	XP_014200351.1	ras-specific guanine nucleotide-releasing factor 2 isoform X3

Pathways from BioSystems
Gene Ontology

The following RASGRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa452660	XM_024928740.1 Latest version!	Pan paniscus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPa49830	XM_003822263.2 Latest version!	Pan paniscus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa49831	XM_014344865.2 Latest version!	Pan paniscus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OPa49831	XM_014344865.1	Pan paniscus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGRF2 ( XM_024928740.1 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_024784508.1 Pan paniscus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa452660
Clone ID Related Accession (Same CDS sequence)	XM_024928740.1
Accession Version	XM_024928740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525017600000
Organism	Pan paniscus(pygmy chimpanzee)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_027873.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan paniscus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCCGGG AGGCGTCCGA GATGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GATGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA CGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TCAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCGAACTG GCCCACTTTG 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTGTCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACGTTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCCGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGCCAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGGCTT TAACAACACC 
GAGAGAACAT GTGATAAAGA GTTTATTATA CGGAGAACGG CTACCAATCG AGTTCTGAAC 
GTCCTCCGTC ACTGGGTCTC AAAGCACGCA CAGGATTTCG AACTCAACAA TGAACTAAAG 
ATGAATGTCC TAAATTTGCT AGAAGAAGTT TTGCGAGACC CAGACCTTCT TCCCCAAGAA 
AGGAAAGCCA CCGCGAATAT CCTCAGGGCT CTTTCACAAG ATGACCAAGA TGACATCCAC 
CTAAAATTAG AGGATATAAT TCAAATGACT GACTGCATGA AGGCCGAATG CTTTGAGTCC 
TTGTCGGCCA TGGAGCTGGC AGAACAGATC ACCCTCCTGG ACCATGTCAT TTTCAGAAGC 
ATTCCCTACG AGGAGTTTCT TGGGCAGGGG TGGATGAAGC TGGATAAAAA CGAAAGAACT 
CCTTACATTA TGAAAACCAG CCAACACTTC AATGACATGA GTAACCTGGT GGCCTCCCAG 
ATAATGAACT ATGCTGATGT CAGCTCCCGT GCCAACGCCA TCGAGAAATG GGTGGCAGTG 
GCGGACATCT GCCGATGCCT GCACAACTAC AACGGCGTGC TGGAGATCAC CTCGGCCTTA 
AACAGAAGTG CCATCTACAG GCTGAAGAAA ACCTGGGCCA AGGTCTCTAA GCAGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024784508.1
CDS	59..3418
Translation	MQKSVRYNEGHALYLAFLARKEGTKRGFLSKKTAEASRWHEKWFALYQNVLFYFEGEQSCRPAGMYLLEGCSCERTPAPPRAGAGPGGVRDALDKQYYFTVLFGHEGQKPLELRCEEEQDGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIDCTLIEEPDASDDDSKGSGQVFGHLDFKIVVEPPDAAAFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNRGEHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSKIGALDLTTSSSPTTTTQSPAASPPPHTGQIPLDLSRGLSSPEQSPGTVEENVDNPRVDLCNKLKRSIQKAVLESAPADRAGVESSPAADTTELSPCRSPSTPRHLRYRQPGGQTADNAHCSVSPASAFAIATAAAGHGSPPGFNNTERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQMTDCMKAECFESLSAMELAEQITLLDHVIFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVSSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQL

Target ORF information:

RefSeq Version	XM_024928740.1
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024928740.1

ORF Insert Sequence:

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCCGGG AGGCGTCCGA GATGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GATGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA CGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TCAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCGAACTG GCCCACTTTG 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTGTCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACGTTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCCGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGCCAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGGCTT TAACAACACC 
GAGAGAACAT GTGATAAAGA GTTTATTATA CGGAGAACGG CTACCAATCG AGTTCTGAAC 
GTCCTCCGTC ACTGGGTCTC AAAGCACGCA CAGGATTTCG AACTCAACAA TGAACTAAAG 
ATGAATGTCC TAAATTTGCT AGAAGAAGTT TTGCGAGACC CAGACCTTCT TCCCCAAGAA 
AGGAAAGCCA CCGCGAATAT CCTCAGGGCT CTTTCACAAG ATGACCAAGA TGACATCCAC 
CTAAAATTAG AGGATATAAT TCAAATGACT GACTGCATGA AGGCCGAATG CTTTGAGTCC 
TTGTCGGCCA TGGAGCTGGC AGAACAGATC ACCCTCCTGG ACCATGTCAT TTTCAGAAGC 
ATTCCCTACG AGGAGTTTCT TGGGCAGGGG TGGATGAAGC TGGATAAAAA CGAAAGAACT 
CCTTACATTA TGAAAACCAG CCAACACTTC AATGACATGA GTAACCTGGT GGCCTCCCAG 
ATAATGAACT ATGCTGATGT CAGCTCCCGT GCCAACGCCA TCGAGAAATG GGTGGCAGTG 
GCGGACATCT GCCGATGCCT GCACAACTAC AACGGCGTGC TGGAGATCAC CTCGGCCTTA 
AACAGAAGTG CCATCTACAG GCTGAAGAAA ACCTGGGCCA AGGTCTCTAA GCAGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2 ( XM_003822263.2 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_003822311.1 Pan paniscus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa49830
Clone ID Related Accession (Same CDS sequence)	XM_003822263.2
Accession Version	XM_003822263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525017600000
Organism	Pan paniscus(pygmy chimpanzee)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_027873.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 2, 2014 this sequence version replaced XM_003822263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan paniscus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCCGGG AGGCGTCCGA GATGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GATGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA CGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TCAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCGAACTG GCCCACTTTG 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTGTCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACGTTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCCGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGCCAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGGCTT TAACAACACC 
GAGAGAACAT GTGATAAAGA GTTTATTATA CGGAGAACGG CTACCAATCG AGTTCTGAAC 
GTCCTCCGTC ACTGGGTCTC AAAGCACGCA CAGGATTTCG AACTCAACAA TGAACTAAAG 
ATGAATGTCC TAAATTTGCT AGAAGAAGTT TTGCGAGACC CAGACCTTCT TCCCCAAGAA 
AGGAAAGCCA CCGCGAATAT CCTCAGGGCT CTTTCACAAG ATGACCAAGA TGACATCCAC 
CTAAAATTAG AGGATATAAT TCAAATGACT GACTGCATGA AGGCCGAATG CTTTGAGTCC 
TTGTCGGCCA TGGAGCTGGC AGAACAGATC ACCCTCCTGG ACCATGTCAT TTTCAGAAGC 
ATTCCCTACG AGGAGTTTCT TGGGCAGGGG TGGATGAAGC TGGATAAAAA CGAAAGAACT 
CCTTACATTA TGAAAACCAG CCAACACTTC AATGACATGA GTAACCTGGT GGCCTCCCAG 
ATAATGAACT ATGCTGATGT CAGCTCCCGT GCCAACGCCA TCGAGAAATG GGTGGCAGTG 
GCGGACATCT GCCGATGCCT GCACAACTAC AACGGCGTGC TGGAGATCAC CTCGGCCTTA 
AACAGAAGTG CCATCTACAG GCTGAAGAAA ACCTGGGCCA AGGTCTCTAA GCAGACAAAA 
GCTCTAATGG ACAAACTTCA AAAGACTGTT TCCTCTGAAG GAAGATTTAA AAATCTTAGA 
GAAACCCTTA AAAATTGTAA CCCTCCTGCA GTTCCTTATC TTGGGATGTA CTTGACAGAC 
CTGGCATTTA TTGAAGAAGG AACACCAAAC TTTACTGAGG AAGGCCTTGT CAATTTCTCC 
AAAATGAGAA TGATATCACA CATCATCAGA GAGATACGCC AGTTCCAGCA GACTTCCTAC 
AGAATAGATC ATCAGCCAAA GGTCACACAG TACTTGCTTG ACAAAGACCT TATCATAGAT 
GAAGATACGC TATATGAGCT GTCACTAAAA ATTGAACCTC GACTCCCTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003822311.1
CDS	59..3772
Translation	MQKSVRYNEGHALYLAFLARKEGTKRGFLSKKTAEASRWHEKWFALYQNVLFYFEGEQSCRPAGMYLLEGCSCERTPAPPRAGAGPGGVRDALDKQYYFTVLFGHEGQKPLELRCEEEQDGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIDCTLIEEPDASDDDSKGSGQVFGHLDFKIVVEPPDAAAFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNRGEHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSKIGALDLTTSSSPTTTTQSPAASPPPHTGQIPLDLSRGLSSPEQSPGTVEENVDNPRVDLCNKLKRSIQKAVLESAPADRAGVESSPAADTTELSPCRSPSTPRHLRYRQPGGQTADNAHCSVSPASAFAIATAAAGHGSPPGFNNTERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQMTDCMKAECFESLSAMELAEQITLLDHVIFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVSSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVTQYLLDKDLIIDEDTLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_003822263.2
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003822263.2

ORF Insert Sequence:

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCCGGG AGGCGTCCGA GATGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GATGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA CGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TCAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCGAACTG GCCCACTTTG 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTGTCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACGTTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCCGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGCCAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGGCTT TAACAACACC 
GAGAGAACAT GTGATAAAGA GTTTATTATA CGGAGAACGG CTACCAATCG AGTTCTGAAC 
GTCCTCCGTC ACTGGGTCTC AAAGCACGCA CAGGATTTCG AACTCAACAA TGAACTAAAG 
ATGAATGTCC TAAATTTGCT AGAAGAAGTT TTGCGAGACC CAGACCTTCT TCCCCAAGAA 
AGGAAAGCCA CCGCGAATAT CCTCAGGGCT CTTTCACAAG ATGACCAAGA TGACATCCAC 
CTAAAATTAG AGGATATAAT TCAAATGACT GACTGCATGA AGGCCGAATG CTTTGAGTCC 
TTGTCGGCCA TGGAGCTGGC AGAACAGATC ACCCTCCTGG ACCATGTCAT TTTCAGAAGC 
ATTCCCTACG AGGAGTTTCT TGGGCAGGGG TGGATGAAGC TGGATAAAAA CGAAAGAACT 
CCTTACATTA TGAAAACCAG CCAACACTTC AATGACATGA GTAACCTGGT GGCCTCCCAG 
ATAATGAACT ATGCTGATGT CAGCTCCCGT GCCAACGCCA TCGAGAAATG GGTGGCAGTG 
GCGGACATCT GCCGATGCCT GCACAACTAC AACGGCGTGC TGGAGATCAC CTCGGCCTTA 
AACAGAAGTG CCATCTACAG GCTGAAGAAA ACCTGGGCCA AGGTCTCTAA GCAGACAAAA 
GCTCTAATGG ACAAACTTCA AAAGACTGTT TCCTCTGAAG GAAGATTTAA AAATCTTAGA 
GAAACCCTTA AAAATTGTAA CCCTCCTGCA GTTCCTTATC TTGGGATGTA CTTGACAGAC 
CTGGCATTTA TTGAAGAAGG AACACCAAAC TTTACTGAGG AAGGCCTTGT CAATTTCTCC 
AAAATGAGAA TGATATCACA CATCATCAGA GAGATACGCC AGTTCCAGCA GACTTCCTAC 
AGAATAGATC ATCAGCCAAA GGTCACACAG TACTTGCTTG ACAAAGACCT TATCATAGAT 
GAAGATACGC TATATGAGCT GTCACTAAAA ATTGAACCTC GACTCCCTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2 ( XM_014344865.1 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_014200351.1 Pan paniscus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa49831
Clone ID Related Accession (Same CDS sequence)	XM_014344865.1 , XM_014344865.2
Accession Version	XM_014344865.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1443456000000
Organism	Pan paniscus(pygmy chimpanzee)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014013930.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan paniscus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGT ACATTCATCT AGTTCAGATC GTAGAGACAG AAAAAATTGC AGCTAACCAA 
CTCCGACATC AACTTGAAGA TCAAGACACA GAAATCGAAA GGCTTAAATC AGAGATTATT 
GCTCTTAATA AAACCAAAGA ACGAATGCGA CCTTACCAAA GCAACCAAGA AGATGAAGAT 
CCAGACATCA AGAAGATTAA AAAGGTTCAG AGCTTCATGC GAGGATGGTT GTGCAGAAGG 
AAATGGAAGA CCATCGTGCA GGATTACATT TGTTCTCCTC ATGCTGAAAG TATGAGGAAG 
AGAAACCAGA TTGTGTTCAC CATGGTGGAG GCAGAGTCAG AGTACGTTCA CCAGCTCTAC 
ATCCTGGTCA ACGGCTTTCT CCGGCCCCTG CGTATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCAGCCACG ACGACGTCAG CAGTATTTTT CTCAACAGTG AAACAATCAT GTTTCTTCAT 
GAAATATTTC ATCAAGGACT AAAGGCAAGG ATAGCGAACT GGCCCACTTT GATTTTAGCT 
GATCTGTTTG ATATTTTGCT CCCCATGCTG AACATTTATC AAGAATTTGT GCGTAATCAC 
CAGTACAGCC TGCAAGTTCT CGCCAATTGT AAGCAAAACA GAGATTTTGA CAAACTCTTA 
AAACAGTATG AAGCCAATCC AGCCTGTGAG GGGAGGATGC TGGAGACATT CTTGACCTAT 
CCCATGTTTC AGATCCCCAG ATATATCATC ACACTCCATG AGCTCCTTGC TCACACACCC 
CATGAGCATG TGGAAAGGAA AAGCCTGGAG TTTGCCAAAT CAAAGCTAGA GGAACTGTCC 
AGAGTAATGC ACGATGAAGT CAGCGACACT GAAAACATAA GGAAAAACCT TGCCATCGAA 
AGAATGATCG TGGAGGGCTG TGACATCTTG CTGGACACCA GCCAAACGTT CATCCGCCAA 
GGTTCTCTTA TTCAAGTACC TTCCGTTGAG AGGGGGAAAC TTAGTAAAGT TCGCCTGGGT 
TCGTTGTCTT TGAAAAAGGA AGGAGAGAGA CAATGCTTCT TATTTACAAA ACACTTTTTA 
ATATGTACAA GAAGTTCAGG AGGGAAGCTT CATCTGCTCA AGACAGGTGG GGTTCTGTCT 
CTAATAGACT GCACGTTGAT TGAGGAGCCA GATGCAAGCG ATGATGACTC TAAAGGTTCT 
GGGCAAGTGT TTGGGCACCT GGATTTTAAA ATAGTGGTGG AGCCTCCCGA CGCTGCCGCC 
TTCACTGTTG TCTTGTTAGC ACCCTCACGC CAGGAGAAAG CTGCCTGGAT GAGTGACATC 
AGTCAGTGTG TGGACAATAT ACGATGTAAT GGTTTAATGA CTATAGTGTT TGAAGAGAAT 
TCCAAAGTCA CTGTGCCACA TATGATTAAG TCTGATGCCC GTCTTCATAA AGACGACACT 
GACATTTGCT TCAGTAAAAC ACTCAACTCC TGCAAAGTGC CCCAGATCCG TTATGCCAGC 
GTGGAGCGCC TCTTGGAACG ACTGACAGAC TTGCGGTTTC TTAGTATTGA TTTCCTCAAC 
ACCTTTCTGC ACACCTATCG TATTTTCACT ACTGCCGCTG TGGTGCTGGG GAAACTCTCC 
GACATATACA AGAGGCCTTT CACCTCCATC CCTGTCAGGT CATTGGAATT GTTTTTTGCT 
ACCAGCCAGA ACAACAGAGG TGAACATTTG GTGGATGGCA AATCCCCACG TCTGTGTCGC 
AAATTCTCTT CCCCGCCACC ACTGGCTGTG TCCAGAACAT CTTCCCCAGT GAGGGCCAGA 
AAGCTGTCTT TGACTTCTCC CTTGAACTCA AAGATAGGAG CATTGGACCT GACAACTTCC 
AGCAGTCCCA CCACCACCAC CCAGAGTCCC GCTGCGTCTC CACCACCACA CACTGGTCAG 
ATACCACTGG ATCTCAGCAG AGGCCTCTCT TCTCCAGAGC AAAGCCCGGG AACGGTAGAA 
GAGAATGTCG ATAACCCACG CGTGGATCTG TGTAACAAGC TAAAACGAAG TATTCAAAAA 
GCAGTCCTAG AGTCTGCACC AGCGGACCGA GCAGGAGTGG AAAGCTCCCC TGCAGCGGAC 
ACCACAGAAC TTTCACCTTG CAGATCCCCC TCAACTCCTC GGCACCTCCG CTATCGCCAG 
CCTGGAGGAC AGACGGCGGA CAATGCCCAC TGCTCTGTTT CACCGGCTTC TGCTTTTGCA 
ATAGCCACAG CTGCAGCAGG ACATGGGAGT CCACCAGGCT TTAACAACAC CGAGAGAACA 
TGTGATAAAG AGTTTATTAT ACGGAGAACG GCTACCAATC GAGTTCTGAA CGTCCTCCGT 
CACTGGGTCT CAAAGCACGC ACAGGATTTC GAACTCAACA ATGAACTAAA GATGAATGTC 
CTAAATTTGC TAGAAGAAGT TTTGCGAGAC CCAGACCTTC TTCCCCAAGA AAGGAAAGCC 
ACCGCGAATA TCCTCAGGGC TCTTTCACAA GATGACCAAG ATGACATCCA CCTAAAATTA 
GAGGATATAA TTCAAATGAC TGACTGCATG AAGGCCGAAT GCTTTGAGTC CTTGTCGGCC 
ATGGAGCTGG CAGAACAGAT CACCCTCCTG GACCATGTCA TTTTCAGAAG CATTCCCTAC 
GAGGAGTTTC TTGGGCAGGG GTGGATGAAG CTGGATAAAA ACGAAAGAAC TCCTTACATT 
ATGAAAACCA GCCAACACTT CAATGACATG AGTAACCTGG TGGCCTCCCA GATAATGAAC 
TATGCTGATG TCAGCTCCCG TGCCAACGCC ATCGAGAAAT GGGTGGCAGT GGCGGACATC 
TGCCGATGCC TGCACAACTA CAACGGCGTG CTGGAGATCA CCTCGGCCTT AAACAGAAGT 
GCCATCTACA GGCTGAAGAA AACCTGGGCC AAGGTCTCTA AGCAGACAAA AGCTCTAATG 
GACAAACTTC AAAAGACTGT TTCCTCTGAA GGAAGATTTA AAAATCTTAG AGAAACCCTT 
AAAAATTGTA ACCCTCCTGC AGTTCCTTAT CTTGGGATGT ACTTGACAGA CCTGGCATTT 
ATTGAAGAAG GAACACCAAA CTTTACTGAG GAAGGCCTTG TCAATTTCTC CAAAATGAGA 
ATGATATCAC ACATCATCAG AGAGATACGC CAGTTCCAGC AGACTTCCTA CAGAATAGAT 
CATCAGCCAA AGGTCACACA GTACTTGCTT GACAAAGACC TTATCATAGA TGAAGATACG 
CTATATGAGC TGTCACTAAA AATTGAACCT CGACTCCCTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014200351.1
CDS	87..3371
Translation	MQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIDCTLIEEPDASDDDSKGSGQVFGHLDFKIVVEPPDAAAFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNRGEHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSKIGALDLTTSSSPTTTTQSPAASPPPHTGQIPLDLSRGLSSPEQSPGTVEENVDNPRVDLCNKLKRSIQKAVLESAPADRAGVESSPAADTTELSPCRSPSTPRHLRYRQPGGQTADNAHCSVSPASAFAIATAAAGHGSPPGFNNTERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQMTDCMKAECFESLSAMELAEQITLLDHVIFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVSSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVTQYLLDKDLIIDEDTLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_014344865.1
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014344865.1

ORF Insert Sequence:

ATGCAGAAGT ACATTCATCT AGTTCAGATC GTAGAGACAG AAAAAATTGC AGCTAACCAA 
CTCCGACATC AACTTGAAGA TCAAGACACA GAAATCGAAA GGCTTAAATC AGAGATTATT 
GCTCTTAATA AAACCAAAGA ACGAATGCGA CCTTACCAAA GCAACCAAGA AGATGAAGAT 
CCAGACATCA AGAAGATTAA AAAGGTTCAG AGCTTCATGC GAGGATGGTT GTGCAGAAGG 
AAATGGAAGA CCATCGTGCA GGATTACATT TGTTCTCCTC ATGCTGAAAG TATGAGGAAG 
AGAAACCAGA TTGTGTTCAC CATGGTGGAG GCAGAGTCAG AGTACGTTCA CCAGCTCTAC 
ATCCTGGTCA ACGGCTTTCT CCGGCCCCTG CGTATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCAGCCACG ACGACGTCAG CAGTATTTTT CTCAACAGTG AAACAATCAT GTTTCTTCAT 
GAAATATTTC ATCAAGGACT AAAGGCAAGG ATAGCGAACT GGCCCACTTT GATTTTAGCT 
GATCTGTTTG ATATTTTGCT CCCCATGCTG AACATTTATC AAGAATTTGT GCGTAATCAC 
CAGTACAGCC TGCAAGTTCT CGCCAATTGT AAGCAAAACA GAGATTTTGA CAAACTCTTA 
AAACAGTATG AAGCCAATCC AGCCTGTGAG GGGAGGATGC TGGAGACATT CTTGACCTAT 
CCCATGTTTC AGATCCCCAG ATATATCATC ACACTCCATG AGCTCCTTGC TCACACACCC 
CATGAGCATG TGGAAAGGAA AAGCCTGGAG TTTGCCAAAT CAAAGCTAGA GGAACTGTCC 
AGAGTAATGC ACGATGAAGT CAGCGACACT GAAAACATAA GGAAAAACCT TGCCATCGAA 
AGAATGATCG TGGAGGGCTG TGACATCTTG CTGGACACCA GCCAAACGTT CATCCGCCAA 
GGTTCTCTTA TTCAAGTACC TTCCGTTGAG AGGGGGAAAC TTAGTAAAGT TCGCCTGGGT 
TCGTTGTCTT TGAAAAAGGA AGGAGAGAGA CAATGCTTCT TATTTACAAA ACACTTTTTA 
ATATGTACAA GAAGTTCAGG AGGGAAGCTT CATCTGCTCA AGACAGGTGG GGTTCTGTCT 
CTAATAGACT GCACGTTGAT TGAGGAGCCA GATGCAAGCG ATGATGACTC TAAAGGTTCT 
GGGCAAGTGT TTGGGCACCT GGATTTTAAA ATAGTGGTGG AGCCTCCCGA CGCTGCCGCC 
TTCACTGTTG TCTTGTTAGC ACCCTCACGC CAGGAGAAAG CTGCCTGGAT GAGTGACATC 
AGTCAGTGTG TGGACAATAT ACGATGTAAT GGTTTAATGA CTATAGTGTT TGAAGAGAAT 
TCCAAAGTCA CTGTGCCACA TATGATTAAG TCTGATGCCC GTCTTCATAA AGACGACACT 
GACATTTGCT TCAGTAAAAC ACTCAACTCC TGCAAAGTGC CCCAGATCCG TTATGCCAGC 
GTGGAGCGCC TCTTGGAACG ACTGACAGAC TTGCGGTTTC TTAGTATTGA TTTCCTCAAC 
ACCTTTCTGC ACACCTATCG TATTTTCACT ACTGCCGCTG TGGTGCTGGG GAAACTCTCC 
GACATATACA AGAGGCCTTT CACCTCCATC CCTGTCAGGT CATTGGAATT GTTTTTTGCT 
ACCAGCCAGA ACAACAGAGG TGAACATTTG GTGGATGGCA AATCCCCACG TCTGTGTCGC 
AAATTCTCTT CCCCGCCACC ACTGGCTGTG TCCAGAACAT CTTCCCCAGT GAGGGCCAGA 
AAGCTGTCTT TGACTTCTCC CTTGAACTCA AAGATAGGAG CATTGGACCT GACAACTTCC 
AGCAGTCCCA CCACCACCAC CCAGAGTCCC GCTGCGTCTC CACCACCACA CACTGGTCAG 
ATACCACTGG ATCTCAGCAG AGGCCTCTCT TCTCCAGAGC AAAGCCCGGG AACGGTAGAA 
GAGAATGTCG ATAACCCACG CGTGGATCTG TGTAACAAGC TAAAACGAAG TATTCAAAAA 
GCAGTCCTAG AGTCTGCACC AGCGGACCGA GCAGGAGTGG AAAGCTCCCC TGCAGCGGAC 
ACCACAGAAC TTTCACCTTG CAGATCCCCC TCAACTCCTC GGCACCTCCG CTATCGCCAG 
CCTGGAGGAC AGACGGCGGA CAATGCCCAC TGCTCTGTTT CACCGGCTTC TGCTTTTGCA 
ATAGCCACAG CTGCAGCAGG ACATGGGAGT CCACCAGGCT TTAACAACAC CGAGAGAACA 
TGTGATAAAG AGTTTATTAT ACGGAGAACG GCTACCAATC GAGTTCTGAA CGTCCTCCGT 
CACTGGGTCT CAAAGCACGC ACAGGATTTC GAACTCAACA ATGAACTAAA GATGAATGTC 
CTAAATTTGC TAGAAGAAGT TTTGCGAGAC CCAGACCTTC TTCCCCAAGA AAGGAAAGCC 
ACCGCGAATA TCCTCAGGGC TCTTTCACAA GATGACCAAG ATGACATCCA CCTAAAATTA 
GAGGATATAA TTCAAATGAC TGACTGCATG AAGGCCGAAT GCTTTGAGTC CTTGTCGGCC 
ATGGAGCTGG CAGAACAGAT CACCCTCCTG GACCATGTCA TTTTCAGAAG CATTCCCTAC 
GAGGAGTTTC TTGGGCAGGG GTGGATGAAG CTGGATAAAA ACGAAAGAAC TCCTTACATT 
ATGAAAACCA GCCAACACTT CAATGACATG AGTAACCTGG TGGCCTCCCA GATAATGAAC 
TATGCTGATG TCAGCTCCCG TGCCAACGCC ATCGAGAAAT GGGTGGCAGT GGCGGACATC 
TGCCGATGCC TGCACAACTA CAACGGCGTG CTGGAGATCA CCTCGGCCTT AAACAGAAGT 
GCCATCTACA GGCTGAAGAA AACCTGGGCC AAGGTCTCTA AGCAGACAAA AGCTCTAATG 
GACAAACTTC AAAAGACTGT TTCCTCTGAA GGAAGATTTA AAAATCTTAG AGAAACCCTT 
AAAAATTGTA ACCCTCCTGC AGTTCCTTAT CTTGGGATGT ACTTGACAGA CCTGGCATTT 
ATTGAAGAAG GAACACCAAA CTTTACTGAG GAAGGCCTTG TCAATTTCTC CAAAATGAGA 
ATGATATCAC ACATCATCAG AGAGATACGC CAGTTCCAGC AGACTTCCTA CAGAATAGAT 
CATCAGCCAA AGGTCACACA GTACTTGCTT GACAAAGACC TTATCATAGA TGAAGATACG 
CTATATGAGC TGTCACTAAA AATTGAACCT CGACTCCCTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2 ( XM_014344865.2 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_014200351.1 Pan paniscus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPa49831
Clone ID Related Accession (Same CDS sequence)	XM_014344865.1 , XM_014344865.2
Accession Version	XM_014344865.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525017600000
Organism	Pan paniscus(pygmy chimpanzee)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_027873.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 1, 2018 this sequence version replaced XM_014344865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan paniscus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGT ACATTCATCT AGTTCAGATC GTAGAGACAG AAAAAATTGC AGCTAACCAA 
CTCCGACATC AACTTGAAGA TCAAGACACA GAAATCGAAA GGCTTAAATC AGAGATTATT 
GCTCTTAATA AAACCAAAGA ACGAATGCGA CCTTACCAAA GCAACCAAGA AGATGAAGAT 
CCAGACATCA AGAAGATTAA AAAGGTTCAG AGCTTCATGC GAGGATGGTT GTGCAGAAGG 
AAATGGAAGA CCATCGTGCA GGATTACATT TGTTCTCCTC ATGCTGAAAG TATGAGGAAG 
AGAAACCAGA TTGTGTTCAC CATGGTGGAG GCAGAGTCAG AGTACGTTCA CCAGCTCTAC 
ATCCTGGTCA ACGGCTTTCT CCGGCCCCTG CGTATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCAGCCACG ACGACGTCAG CAGTATTTTT CTCAACAGTG AAACAATCAT GTTTCTTCAT 
GAAATATTTC ATCAAGGACT AAAGGCAAGG ATAGCGAACT GGCCCACTTT GATTTTAGCT 
GATCTGTTTG ATATTTTGCT CCCCATGCTG AACATTTATC AAGAATTTGT GCGTAATCAC 
CAGTACAGCC TGCAAGTTCT CGCCAATTGT AAGCAAAACA GAGATTTTGA CAAACTCTTA 
AAACAGTATG AAGCCAATCC AGCCTGTGAG GGGAGGATGC TGGAGACATT CTTGACCTAT 
CCCATGTTTC AGATCCCCAG ATATATCATC ACACTCCATG AGCTCCTTGC TCACACACCC 
CATGAGCATG TGGAAAGGAA AAGCCTGGAG TTTGCCAAAT CAAAGCTAGA GGAACTGTCC 
AGAGTAATGC ACGATGAAGT CAGCGACACT GAAAACATAA GGAAAAACCT TGCCATCGAA 
AGAATGATCG TGGAGGGCTG TGACATCTTG CTGGACACCA GCCAAACGTT CATCCGCCAA 
GGTTCTCTTA TTCAAGTACC TTCCGTTGAG AGGGGGAAAC TTAGTAAAGT TCGCCTGGGT 
TCGTTGTCTT TGAAAAAGGA AGGAGAGAGA CAATGCTTCT TATTTACAAA ACACTTTTTA 
ATATGTACAA GAAGTTCAGG AGGGAAGCTT CATCTGCTCA AGACAGGTGG GGTTCTGTCT 
CTAATAGACT GCACGTTGAT TGAGGAGCCA GATGCAAGCG ATGATGACTC TAAAGGTTCT 
GGGCAAGTGT TTGGGCACCT GGATTTTAAA ATAGTGGTGG AGCCTCCCGA CGCTGCCGCC 
TTCACTGTTG TCTTGTTAGC ACCCTCACGC CAGGAGAAAG CTGCCTGGAT GAGTGACATC 
AGTCAGTGTG TGGACAATAT ACGATGTAAT GGTTTAATGA CTATAGTGTT TGAAGAGAAT 
TCCAAAGTCA CTGTGCCACA TATGATTAAG TCTGATGCCC GTCTTCATAA AGACGACACT 
GACATTTGCT TCAGTAAAAC ACTCAACTCC TGCAAAGTGC CCCAGATCCG TTATGCCAGC 
GTGGAGCGCC TCTTGGAACG ACTGACAGAC TTGCGGTTTC TTAGTATTGA TTTCCTCAAC 
ACCTTTCTGC ACACCTATCG TATTTTCACT ACTGCCGCTG TGGTGCTGGG GAAACTCTCC 
GACATATACA AGAGGCCTTT CACCTCCATC CCTGTCAGGT CATTGGAATT GTTTTTTGCT 
ACCAGCCAGA ACAACAGAGG TGAACATTTG GTGGATGGCA AATCCCCACG TCTGTGTCGC 
AAATTCTCTT CCCCGCCACC ACTGGCTGTG TCCAGAACAT CTTCCCCAGT GAGGGCCAGA 
AAGCTGTCTT TGACTTCTCC CTTGAACTCA AAGATAGGAG CATTGGACCT GACAACTTCC 
AGCAGTCCCA CCACCACCAC CCAGAGTCCC GCTGCGTCTC CACCACCACA CACTGGTCAG 
ATACCACTGG ATCTCAGCAG AGGCCTCTCT TCTCCAGAGC AAAGCCCGGG AACGGTAGAA 
GAGAATGTCG ATAACCCACG CGTGGATCTG TGTAACAAGC TAAAACGAAG TATTCAAAAA 
GCAGTCCTAG AGTCTGCACC AGCGGACCGA GCAGGAGTGG AAAGCTCCCC TGCAGCGGAC 
ACCACAGAAC TTTCACCTTG CAGATCCCCC TCAACTCCTC GGCACCTCCG CTATCGCCAG 
CCTGGAGGAC AGACGGCGGA CAATGCCCAC TGCTCTGTTT CACCGGCTTC TGCTTTTGCA 
ATAGCCACAG CTGCAGCAGG ACATGGGAGT CCACCAGGCT TTAACAACAC CGAGAGAACA 
TGTGATAAAG AGTTTATTAT ACGGAGAACG GCTACCAATC GAGTTCTGAA CGTCCTCCGT 
CACTGGGTCT CAAAGCACGC ACAGGATTTC GAACTCAACA ATGAACTAAA GATGAATGTC 
CTAAATTTGC TAGAAGAAGT TTTGCGAGAC CCAGACCTTC TTCCCCAAGA AAGGAAAGCC 
ACCGCGAATA TCCTCAGGGC TCTTTCACAA GATGACCAAG ATGACATCCA CCTAAAATTA 
GAGGATATAA TTCAAATGAC TGACTGCATG AAGGCCGAAT GCTTTGAGTC CTTGTCGGCC 
ATGGAGCTGG CAGAACAGAT CACCCTCCTG GACCATGTCA TTTTCAGAAG CATTCCCTAC 
GAGGAGTTTC TTGGGCAGGG GTGGATGAAG CTGGATAAAA ACGAAAGAAC TCCTTACATT 
ATGAAAACCA GCCAACACTT CAATGACATG AGTAACCTGG TGGCCTCCCA GATAATGAAC 
TATGCTGATG TCAGCTCCCG TGCCAACGCC ATCGAGAAAT GGGTGGCAGT GGCGGACATC 
TGCCGATGCC TGCACAACTA CAACGGCGTG CTGGAGATCA CCTCGGCCTT AAACAGAAGT 
GCCATCTACA GGCTGAAGAA AACCTGGGCC AAGGTCTCTA AGCAGACAAA AGCTCTAATG 
GACAAACTTC AAAAGACTGT TTCCTCTGAA GGAAGATTTA AAAATCTTAG AGAAACCCTT 
AAAAATTGTA ACCCTCCTGC AGTTCCTTAT CTTGGGATGT ACTTGACAGA CCTGGCATTT 
ATTGAAGAAG GAACACCAAA CTTTACTGAG GAAGGCCTTG TCAATTTCTC CAAAATGAGA 
ATGATATCAC ACATCATCAG AGAGATACGC CAGTTCCAGC AGACTTCCTA CAGAATAGAT 
CATCAGCCAA AGGTCACACA GTACTTGCTT GACAAAGACC TTATCATAGA TGAAGATACG 
CTATATGAGC TGTCACTAAA AATTGAACCT CGACTCCCTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014200351.1
CDS	56..3340
Translation	MQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIDCTLIEEPDASDDDSKGSGQVFGHLDFKIVVEPPDAAAFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNRGEHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSKIGALDLTTSSSPTTTTQSPAASPPPHTGQIPLDLSRGLSSPEQSPGTVEENVDNPRVDLCNKLKRSIQKAVLESAPADRAGVESSPAADTTELSPCRSPSTPRHLRYRQPGGQTADNAHCSVSPASAFAIATAAAGHGSPPGFNNTERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQMTDCMKAECFESLSAMELAEQITLLDHVIFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVSSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVTQYLLDKDLIIDEDTLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_014344865.2
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014344865.2

ORF Insert Sequence:

ATGCAGAAGT ACATTCATCT AGTTCAGATC GTAGAGACAG AAAAAATTGC AGCTAACCAA 
CTCCGACATC AACTTGAAGA TCAAGACACA GAAATCGAAA GGCTTAAATC AGAGATTATT 
GCTCTTAATA AAACCAAAGA ACGAATGCGA CCTTACCAAA GCAACCAAGA AGATGAAGAT 
CCAGACATCA AGAAGATTAA AAAGGTTCAG AGCTTCATGC GAGGATGGTT GTGCAGAAGG 
AAATGGAAGA CCATCGTGCA GGATTACATT TGTTCTCCTC ATGCTGAAAG TATGAGGAAG 
AGAAACCAGA TTGTGTTCAC CATGGTGGAG GCAGAGTCAG AGTACGTTCA CCAGCTCTAC 
ATCCTGGTCA ACGGCTTTCT CCGGCCCCTG CGTATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCAGCCACG ACGACGTCAG CAGTATTTTT CTCAACAGTG AAACAATCAT GTTTCTTCAT 
GAAATATTTC ATCAAGGACT AAAGGCAAGG ATAGCGAACT GGCCCACTTT GATTTTAGCT 
GATCTGTTTG ATATTTTGCT CCCCATGCTG AACATTTATC AAGAATTTGT GCGTAATCAC 
CAGTACAGCC TGCAAGTTCT CGCCAATTGT AAGCAAAACA GAGATTTTGA CAAACTCTTA 
AAACAGTATG AAGCCAATCC AGCCTGTGAG GGGAGGATGC TGGAGACATT CTTGACCTAT 
CCCATGTTTC AGATCCCCAG ATATATCATC ACACTCCATG AGCTCCTTGC TCACACACCC 
CATGAGCATG TGGAAAGGAA AAGCCTGGAG TTTGCCAAAT CAAAGCTAGA GGAACTGTCC 
AGAGTAATGC ACGATGAAGT CAGCGACACT GAAAACATAA GGAAAAACCT TGCCATCGAA 
AGAATGATCG TGGAGGGCTG TGACATCTTG CTGGACACCA GCCAAACGTT CATCCGCCAA 
GGTTCTCTTA TTCAAGTACC TTCCGTTGAG AGGGGGAAAC TTAGTAAAGT TCGCCTGGGT 
TCGTTGTCTT TGAAAAAGGA AGGAGAGAGA CAATGCTTCT TATTTACAAA ACACTTTTTA 
ATATGTACAA GAAGTTCAGG AGGGAAGCTT CATCTGCTCA AGACAGGTGG GGTTCTGTCT 
CTAATAGACT GCACGTTGAT TGAGGAGCCA GATGCAAGCG ATGATGACTC TAAAGGTTCT 
GGGCAAGTGT TTGGGCACCT GGATTTTAAA ATAGTGGTGG AGCCTCCCGA CGCTGCCGCC 
TTCACTGTTG TCTTGTTAGC ACCCTCACGC CAGGAGAAAG CTGCCTGGAT GAGTGACATC 
AGTCAGTGTG TGGACAATAT ACGATGTAAT GGTTTAATGA CTATAGTGTT TGAAGAGAAT 
TCCAAAGTCA CTGTGCCACA TATGATTAAG TCTGATGCCC GTCTTCATAA AGACGACACT 
GACATTTGCT TCAGTAAAAC ACTCAACTCC TGCAAAGTGC CCCAGATCCG TTATGCCAGC 
GTGGAGCGCC TCTTGGAACG ACTGACAGAC TTGCGGTTTC TTAGTATTGA TTTCCTCAAC 
ACCTTTCTGC ACACCTATCG TATTTTCACT ACTGCCGCTG TGGTGCTGGG GAAACTCTCC 
GACATATACA AGAGGCCTTT CACCTCCATC CCTGTCAGGT CATTGGAATT GTTTTTTGCT 
ACCAGCCAGA ACAACAGAGG TGAACATTTG GTGGATGGCA AATCCCCACG TCTGTGTCGC 
AAATTCTCTT CCCCGCCACC ACTGGCTGTG TCCAGAACAT CTTCCCCAGT GAGGGCCAGA 
AAGCTGTCTT TGACTTCTCC CTTGAACTCA AAGATAGGAG CATTGGACCT GACAACTTCC 
AGCAGTCCCA CCACCACCAC CCAGAGTCCC GCTGCGTCTC CACCACCACA CACTGGTCAG 
ATACCACTGG ATCTCAGCAG AGGCCTCTCT TCTCCAGAGC AAAGCCCGGG AACGGTAGAA 
GAGAATGTCG ATAACCCACG CGTGGATCTG TGTAACAAGC TAAAACGAAG TATTCAAAAA 
GCAGTCCTAG AGTCTGCACC AGCGGACCGA GCAGGAGTGG AAAGCTCCCC TGCAGCGGAC 
ACCACAGAAC TTTCACCTTG CAGATCCCCC TCAACTCCTC GGCACCTCCG CTATCGCCAG 
CCTGGAGGAC AGACGGCGGA CAATGCCCAC TGCTCTGTTT CACCGGCTTC TGCTTTTGCA 
ATAGCCACAG CTGCAGCAGG ACATGGGAGT CCACCAGGCT TTAACAACAC CGAGAGAACA 
TGTGATAAAG AGTTTATTAT ACGGAGAACG GCTACCAATC GAGTTCTGAA CGTCCTCCGT 
CACTGGGTCT CAAAGCACGC ACAGGATTTC GAACTCAACA ATGAACTAAA GATGAATGTC 
CTAAATTTGC TAGAAGAAGT TTTGCGAGAC CCAGACCTTC TTCCCCAAGA AAGGAAAGCC 
ACCGCGAATA TCCTCAGGGC TCTTTCACAA GATGACCAAG ATGACATCCA CCTAAAATTA 
GAGGATATAA TTCAAATGAC TGACTGCATG AAGGCCGAAT GCTTTGAGTC CTTGTCGGCC 
ATGGAGCTGG CAGAACAGAT CACCCTCCTG GACCATGTCA TTTTCAGAAG CATTCCCTAC 
GAGGAGTTTC TTGGGCAGGG GTGGATGAAG CTGGATAAAA ACGAAAGAAC TCCTTACATT 
ATGAAAACCA GCCAACACTT CAATGACATG AGTAACCTGG TGGCCTCCCA GATAATGAAC 
TATGCTGATG TCAGCTCCCG TGCCAACGCC ATCGAGAAAT GGGTGGCAGT GGCGGACATC 
TGCCGATGCC TGCACAACTA CAACGGCGTG CTGGAGATCA CCTCGGCCTT AAACAGAAGT 
GCCATCTACA GGCTGAAGAA AACCTGGGCC AAGGTCTCTA AGCAGACAAA AGCTCTAATG 
GACAAACTTC AAAAGACTGT TTCCTCTGAA GGAAGATTTA AAAATCTTAG AGAAACCCTT 
AAAAATTGTA ACCCTCCTGC AGTTCCTTAT CTTGGGATGT ACTTGACAGA CCTGGCATTT 
ATTGAAGAAG GAACACCAAA CTTTACTGAG GAAGGCCTTG TCAATTTCTC CAAAATGAGA 
ATGATATCAC ACATCATCAG AGAGATACGC CAGTTCCAGC AGACTTCCTA CAGAATAGAT 
CATCAGCCAA AGGTCACACA GTACTTGCTT GACAAAGACC TTATCATAGA TGAAGATACG 
CTATATGAGC TGTCACTAAA AATTGAACCT CGACTCCCTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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RASGRF2 cDNA ORF clone, Pan paniscus(pygmy chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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