NWD1 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	NWD1
Entrez Gene ID	101114951
Full Name	NACHT and WD repeat domain containing 1
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012128875.2	XP_011984265.1	NACHT domain- and WD repeat-containing protein 1 isoform X2
XM_012128876.2	XP_011984266.1	NACHT domain- and WD repeat-containing protein 1 isoform X3
XM_027969461.1	XP_027825262.1	LOW QUALITY PROTEIN: NACHT domain- and WD repeat-containing protein 1
XM_012177722.2	XP_012033112.2	LOW QUALITY PROTEIN: NACHT domain- and WD repeat-containing protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following NWD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NWD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv12827	XM_012128875.2 Latest version!	Ovis aries musimon NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOv12828	XM_012128876.2 Latest version!	Ovis aries musimon NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm49596	XM_012177722.2 Latest version!	Ovis aries NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm80112	XM_027969461.1 Latest version!	Ovis aries NACHT and WD repeat domain containing 1 (NWD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NWD1 ( XM_012128875.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011984265.1 Ovis aries musimon NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOv12827
Clone ID Related Accession (Same CDS sequence)	XM_012128875.2
Accession Version	XM_012128875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942348.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012128875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC TTGCAGATAT GGATGCAGAG AGGGAAGCCC TACAGAGCAC CGCCTACCCT 
GAAGTGCAGA CCTTCTGCCA GAAGCACGGC CTGATGTTCG AGGTGGTTGA CCTGCGGTGG 
GGTATTCGGA ACACTGAGGC CACTAACCCC TTGACTACAG AAATCTGCTT GGAGGAGCTG 
GACCGGTGTC GGGAAATATC CATTGGGCCA GCCTTTGTTG CCCTCGTGGG TGACCAGTAC 
GGTCCCTGCC CAGTCCCCAC ACGGATCGAG GAAAAGGAGT GGGAGGCGCT GAAGGCTCAG 
CTGACCACCA GGCCCCGCGA CCTGGAGCTG GTGGCACGAA GCTTCTGGAG GGACGAGAAC 
ACGGTGCCCC CTGCCTACCT GCTGCAGGCC CTGGGCACTG GGGAGGCCTC TGGGCCTGAG 
GAGGCCACCC TGATCTCTGC ACTGCGCTCT GGAGCCCAGG AGGCCCACAG ACTGGGGCTT 
ATCAGCCAGG AGCAGTGGCA CCGCTACTAC CGGTCAGTCA TCGAGTGGGA AATAGAACGG 
GCCCTGCTGA GCTCAACGGT TGGGGATCAC GGGGCAACAG TCTTCCTGAG AGATATCCAA 
GGCCTCAATA AACACATCCT GGAAGACTGT GCCCTCAAAA TGGTGGACCG TCTTGCAGAT 
GGTTGCCTGG ACACGGATGC CCAGAACCTT CTCAGCAACC TCAAGGGGCG CATTGCTGAC 
CGGCAGCCTG GAGTTCTCAA GGCCCACCAC CTAGCGTGGA GCCGAGACCT GGTGAACCCC 
AAAAACAAGG CTCATGCCCG ATACCTGAAG GAGCTGGGGG AGCAGTTTGT GGCCAGGGCC 
AACCATCAGG TTCTTGAGCG CCTCCGGGAG CTGGAGGCAG GCAGGCAGGA GTTGGCGTGG 
CTCTACCAGG AGATCCGCCA CCACCTGGGA CTCAGTGCCG AGGTCAACCG GACCTTCTGT 
GGACGCCAGG AGCTCCTGAC CCAGCTGGGG CAGCGACTGC GACAGAGTGA TGGCCGCCCC 
CACCCACCCC TGGTGCTTTT TGGACCCGCA GGCATCGGAA AGACGGCTCT GATGTGCAAG 
CTGGCTGAGC AGATGTCGGG GCTGCTCGGC CGCAAGACAG TAACCATTCT GCGGCTTCTG 
GGGACATCTC AGATGAGCTC TGAGGCCCGA GGCCTGCTCC AGAGCATCTG CTTCCAGGTG 
TGCCTGGCCT ATGGGCTGCC CCTGCCCCCT GCCCAGGTCC TAGAGGCCCA CACCAGGCTG 
GTCCAGTTTT TCCACATCCT CCTCCACACC GTCTCCAGCC GAAACTTTGA GTCCCTTGTG 
ATCCTGCTGG ATTCGGTGGA TGAGGTGGAC TCTGTCCACT GTGCTAGGAG GATCCCCTGG 
CTGCCTCCCA AATGTCCTCC AAGGGTCCAC CTCATCGTCT CGGCCTGCTC TGGACAGCGG 
GGGGCTTTAG ACACCATGCG GCAGGTGCTC ACGGACCAAG GGGCTTACTG GGAGGTGAAA 
CCCCTCTCTG GAAACCAAGG GCAAGAGATG ATCCAGCTCC TGCTGGCCGC CTCGAGGAGG 
ACACTGAGTC AGGTGCAGAG GGACATGCTC TGGGCCAGCC TCCCAGAGTG TGGACACCCA 
GGGCGGCTGA GGCTGGCTTT CGAGGAGGCC CGGAAGTGGG CCTCCTTCAC TGTGCCAGCC 
CCACTGGCCT CCACTGCTAA GGAGGCGATG CAACAGCTGT GTGCCCGCCT GGAGCAGACA 
CACGGGCGGC TCCTGGTGGC CCGCGTGCTG GGCTACATTG TGTCTTCCCG GCATGGGCTC 
TCAGAGGCGG AGCTAAAGGA TGTCCTGTCC TTGGATGATG AGGTCCTGCA GAGCGTGTAC 
CAAGACTGGA CCCCACCCAG CAAACAGCTT CTGCGCTTCC CGCCCCTGCT GTGGGTGAGG 
CTTCGTCGAG ATCTGGGTAA CTGCTTGGCC AGGCGGCCGG TGGACGGCTT CACCCTTCTG 
GCCATTGCCC ACAGACAGCT GGCCGAGGTG GTCCGAGAGC TCTACCTGTC TGGGCCTGAG 
AAAGCCAAGA GTCACGGAGT CTTGGCCGAC TTCTTCTCAG GGGCCTGGAG CCAGGGCATC 
AAGAAACTCA TCACCCTGCC ACTCGTGGGA AAACCCCTGA ACCTGGACCG CAAGGTGGCC 
CCTCAGCCCC TGTGGTTCTC CGACACGGTT GCAAACCTGA GGAAGCTGAA GGAGTTACCC 
TACCACTTAG TCCATGCGGG CCGTATAGAG GAGCTGAAGC AGGAGGTGCT GGGCAACATG 
ACCTGGATTT GCTGCCGGGG CCTCTCTGGG GGCATCGAGG GTCTGCTGGA AGACTTTGAC 
CTGTGTGCCC CGCACATGGA CTGTCCTGAG GTCAGCCTGG TCCGGGAAGC TCTCCAGCTG 
TGCCGCCCGG CAGTGGAGCT CCGAGGCATG GAGAGAAGCG TCCTGTGCAC AGAACTCCTG 
GCCAGACTCC ATTTCTTCAC CACTTCACAT CCCGGGCTGG TGGGACAGCT GTGCCAACAG 
GCACAGAGCT GGCTCCGGAT GTGCCCACAG CCTGTGCTGG TGCCCCTCGG AGGATTCCTC 
CCTCCGCCTG GAGGGCCCCT GCGGGCAACC CTCACCGGCT GTCACAAAGG CATCACTGCC 
ATGGCATGGA GCCTGGAAGA GAAGCTGCTG CTGGTTGGCA CGCAGGAGGG CATGGTGGCT 
GTGTGGGACA TGGAGGAACA GCAGGCAGTC CACATCCTAC CTGGACACAC AGGAGAGGTG 
AGGTGTGTGG AAGTGTTTGC CCAAGGGACA CTTGCCGTCT CTGCCTCGAA AGACCACACC 
TTGCGCTTGT GGGACTTACT CTCTGGCCAG GAGAAATTCA CCATTTGGGA TGGAGGTTCA 
AAAGATCCCA CTGAATCTCA GACCTGGAGC CTTCATGTGG ATGAAGCAAA GAAGGTTGTG 
TATTCAGCTT CAGGCTCAAA GGTCAGTGCT TGGAATCTGG AAACTGCAGA GCAGATTTTC 
CATATCCTGG GGGATGCCTC TGACCCCTGG GTATGCACGG CAGTGCTGGC TTCCCAGGCC 
ACCCTGCTGA CTGTGTCCCG GGGCGGCATG ATCAGTCTGT GGAGCTCAGC CACGGGAACA 
CTGCACAGGC GGCAACGTTT GTCCAGCATC AAAGAAGATA CACTTGCCTG CGGGGTCTCA 
GTCCAGAAAC AAAGAATGAT GGTTACTGGG TCCAGCACAG GCTCCATCTC TTTGGTTTCC 
TCCGAAGGAG ACAGCCTGCT GGAAAAGCTT CCGGAAGCTG TGGGGTTCCT GGTGGTATCT 
GAAGATGAGT CTCTTCTCGC TGCAGGTTTT GGAAGGTCTG TGAGGATATT TTTGGCAGAT 
GCACAGGGAT TTCATCGATT CATGGCCACT GACCTTGAAC ATGAAGATGT GGTAGAGACA 
GCCACTTTTG GTCCTGAAAA CAATCTGATT GTCACTGGGT CCCGGGATGC ACTCATTCAG 
GTGTGGAGTC TGTCAGCCCA GGGGACTCTG CTGGACGTCC TGGACGGTGT CGGGGCCCCG 
GTGAGCCTGC TGGCCTGGGG CGGGGCCTTG GTGGCATCTG CTTCCCGTCA GTCCTCATCT 
TTTAAAGTCT GGGATCTCAC CTGCACTCAG AAGCCGAGGG CCTCTAACCC ATTCCTGGAT 
CGCACTGGCC TCACGGCAGT CTCCCACAAC GGCAGCTACG TCTACTTCCC TAAAACTGGG 
GACAAAAACA AAGTCAGCAT TTGGGCCTTG GCAGAAGGTG AGGAGCAAGA TGCTCTGGAC 
ACCTCCAACG AGGTCAGATG TCTGGAGGTC GCTGAGCAGG CCAATCTCCT TTTCACTGGC 
CTCGTTTCAG GGGTTGTCCT GGTGTTCCCC CTGAATTCCA GACAAGACGT GATGTGTATC 
CCTCCTCCAG AAGCCCGGAA GGCCGTCAAC TGCATGGCTC TCAGTAAGGA CGAGGGGCAC 
CTGGCCATTG CCTATGACAG CACCGTCCTG GTGCTGGACA TCAGCCCGGG GGACCCCTGT 
CCAGTCATCG ATGGGCCAAA ATACACCTTC TATACCCAGC TGCCTGACAC CATAGCCAGT 
GTGGCTGCTC TGGCTGACTA TCGTGTCATC TACGGCATGC TCAACGGCGA CCTCTTCCTT 
TACGAGTGTG TGAATTCCAA GGTGTTCCCT CTGGAGGCCC ATGGGAGCCG AGTCACCTGT 
GTGGAGGTCA GCCACAAGGA GCAGCTGGCA GTCAGCGGGG CTGAGGAAGC CCTACTGTGT 
CTCTGGGACC TGCAGGCGTG CAAGTGGAAA TTCGAGATGA GCTACACGAA TTCTTACTGT 
GTAGGAGTCC AATGTGCTTG CTTCTCCAAG GATGACAAGT ATGTCTATGC AGGCTTGAAA 
GATCGCTCCA TAATTGTCTG GAGTGTGCTG GATGGCACAC TGTTGGCTGT CCAGTTTGTC 
CATGCCGTGG TGAACAGAAT CATCCCAACC TCCAATGGCT TCATTGCCCC CTCCAGACAC 
GGCTATCTCA TCCGTGAGCG TTTCCAGTGC CCTTCGTCAA GACTCTCACA ACAGGACCCT 
CTCAAGAACT TCAAGAAAGC AGTGTGGATG GTCAAATCCA GGCAGAGAGA AGAGCTGGCA 
GCTGCTGCAG GAGCACCCCA GGACTTGAGG TCAGAGAGTG CCCAGGGAAA TGAATCCAAA 
TCAAACAAAC GCTCACAAGT CTGCCTGCTG GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011984265.1
CDS	684..5399
Translation	MPFLADMDAEREALQSTAYPEVQTFCQKHGLMFEVVDLRWGIRNTEATNPLTTEICLEELDRCREISIGPAFVALVGDQYGPCPVPTRIEEKEWEALKAQLTTRPRDLELVARSFWRDENTVPPAYLLQALGTGEASGPEEATLISALRSGAQEAHRLGLISQEQWHRYYRSVIEWEIERALLSSTVGDHGATVFLRDIQGLNKHILEDCALKMVDRLADGCLDTDAQNLLSNLKGRIADRQPGVLKAHHLAWSRDLVNPKNKAHARYLKELGEQFVARANHQVLERLRELEAGRQELAWLYQEIRHHLGLSAEVNRTFCGRQELLTQLGQRLRQSDGRPHPPLVLFGPAGIGKTALMCKLAEQMSGLLGRKTVTILRLLGTSQMSSEARGLLQSICFQVCLAYGLPLPPAQVLEAHTRLVQFFHILLHTVSSRNFESLVILLDSVDEVDSVHCARRIPWLPPKCPPRVHLIVSACSGQRGALDTMRQVLTDQGAYWEVKPLSGNQGQEMIQLLLAASRRTLSQVQRDMLWASLPECGHPGRLRLAFEEARKWASFTVPAPLASTAKEAMQQLCARLEQTHGRLLVARVLGYIVSSRHGLSEAELKDVLSLDDEVLQSVYQDWTPPSKQLLRFPPLLWVRLRRDLGNCLARRPVDGFTLLAIAHRQLAEVVRELYLSGPEKAKSHGVLADFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPYHLVHAGRIEELKQEVLGNMTWICCRGLSGGIEGLLEDFDLCAPHMDCPEVSLVREALQLCRPAVELRGMERSVLCTELLARLHFFTTSHPGLVGQLCQQAQSWLRMCPQPVLVPLGGFLPPPGGPLRATLTGCHKGITAMAWSLEEKLLLVGTQEGMVAVWDMEEQQAVHILPGHTGEVRCVEVFAQGTLAVSASKDHTLRLWDLLSGQEKFTIWDGGSKDPTESQTWSLHVDEAKKVVYSASGSKVSAWNLETAEQIFHILGDASDPWVCTAVLASQATLLTVSRGGMISLWSSATGTLHRRQRLSSIKEDTLACGVSVQKQRMMVTGSSTGSISLVSSEGDSLLEKLPEAVGFLVVSEDESLLAAGFGRSVRIFLADAQGFHRFMATDLEHEDVVETATFGPENNLIVTGSRDALIQVWSLSAQGTLLDVLDGVGAPVSLLAWGGALVASASRQSSSFKVWDLTCTQKPRASNPFLDRTGLTAVSHNGSYVYFPKTGDKNKVSIWALAEGEEQDALDTSNEVRCLEVAEQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAVNCMALSKDEGHLAIAYDSTVLVLDISPGDPCPVIDGPKYTFYTQLPDTIASVAALADYRVIYGMLNGDLFLYECVNSKVFPLEAHGSRVTCVEVSHKEQLAVSGAEEALLCLWDLQACKWKFEMSYTNSYCVGVQCACFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPSRHGYLIRERFQCPSSRLSQQDPLKNFKKAVWMVKSRQREELAAAAGAPQDLRSESAQGNESKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_012128875.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012128875.2

ORF Insert Sequence:

ATGCCGTTCC TTGCAGATAT GGATGCAGAG AGGGAAGCCC TACAGAGCAC CGCCTACCCT 
GAAGTGCAGA CCTTCTGCCA GAAGCACGGC CTGATGTTCG AGGTGGTTGA CCTGCGGTGG 
GGTATTCGGA ACACTGAGGC CACTAACCCC TTGACTACAG AAATCTGCTT GGAGGAGCTG 
GACCGGTGTC GGGAAATATC CATTGGGCCA GCCTTTGTTG CCCTCGTGGG TGACCAGTAC 
GGTCCCTGCC CAGTCCCCAC ACGGATCGAG GAAAAGGAGT GGGAGGCGCT GAAGGCTCAG 
CTGACCACCA GGCCCCGCGA CCTGGAGCTG GTGGCACGAA GCTTCTGGAG GGACGAGAAC 
ACGGTGCCCC CTGCCTACCT GCTGCAGGCC CTGGGCACTG GGGAGGCCTC TGGGCCTGAG 
GAGGCCACCC TGATCTCTGC ACTGCGCTCT GGAGCCCAGG AGGCCCACAG ACTGGGGCTT 
ATCAGCCAGG AGCAGTGGCA CCGCTACTAC CGGTCAGTCA TCGAGTGGGA AATAGAACGG 
GCCCTGCTGA GCTCAACGGT TGGGGATCAC GGGGCAACAG TCTTCCTGAG AGATATCCAA 
GGCCTCAATA AACACATCCT GGAAGACTGT GCCCTCAAAA TGGTGGACCG TCTTGCAGAT 
GGTTGCCTGG ACACGGATGC CCAGAACCTT CTCAGCAACC TCAAGGGGCG CATTGCTGAC 
CGGCAGCCTG GAGTTCTCAA GGCCCACCAC CTAGCGTGGA GCCGAGACCT GGTGAACCCC 
AAAAACAAGG CTCATGCCCG ATACCTGAAG GAGCTGGGGG AGCAGTTTGT GGCCAGGGCC 
AACCATCAGG TTCTTGAGCG CCTCCGGGAG CTGGAGGCAG GCAGGCAGGA GTTGGCGTGG 
CTCTACCAGG AGATCCGCCA CCACCTGGGA CTCAGTGCCG AGGTCAACCG GACCTTCTGT 
GGACGCCAGG AGCTCCTGAC CCAGCTGGGG CAGCGACTGC GACAGAGTGA TGGCCGCCCC 
CACCCACCCC TGGTGCTTTT TGGACCCGCA GGCATCGGAA AGACGGCTCT GATGTGCAAG 
CTGGCTGAGC AGATGTCGGG GCTGCTCGGC CGCAAGACAG TAACCATTCT GCGGCTTCTG 
GGGACATCTC AGATGAGCTC TGAGGCCCGA GGCCTGCTCC AGAGCATCTG CTTCCAGGTG 
TGCCTGGCCT ATGGGCTGCC CCTGCCCCCT GCCCAGGTCC TAGAGGCCCA CACCAGGCTG 
GTCCAGTTTT TCCACATCCT CCTCCACACC GTCTCCAGCC GAAACTTTGA GTCCCTTGTG 
ATCCTGCTGG ATTCGGTGGA TGAGGTGGAC TCTGTCCACT GTGCTAGGAG GATCCCCTGG 
CTGCCTCCCA AATGTCCTCC AAGGGTCCAC CTCATCGTCT CGGCCTGCTC TGGACAGCGG 
GGGGCTTTAG ACACCATGCG GCAGGTGCTC ACGGACCAAG GGGCTTACTG GGAGGTGAAA 
CCCCTCTCTG GAAACCAAGG GCAAGAGATG ATCCAGCTCC TGCTGGCCGC CTCGAGGAGG 
ACACTGAGTC AGGTGCAGAG GGACATGCTC TGGGCCAGCC TCCCAGAGTG TGGACACCCA 
GGGCGGCTGA GGCTGGCTTT CGAGGAGGCC CGGAAGTGGG CCTCCTTCAC TGTGCCAGCC 
CCACTGGCCT CCACTGCTAA GGAGGCGATG CAACAGCTGT GTGCCCGCCT GGAGCAGACA 
CACGGGCGGC TCCTGGTGGC CCGCGTGCTG GGCTACATTG TGTCTTCCCG GCATGGGCTC 
TCAGAGGCGG AGCTAAAGGA TGTCCTGTCC TTGGATGATG AGGTCCTGCA GAGCGTGTAC 
CAAGACTGGA CCCCACCCAG CAAACAGCTT CTGCGCTTCC CGCCCCTGCT GTGGGTGAGG 
CTTCGTCGAG ATCTGGGTAA CTGCTTGGCC AGGCGGCCGG TGGACGGCTT CACCCTTCTG 
GCCATTGCCC ACAGACAGCT GGCCGAGGTG GTCCGAGAGC TCTACCTGTC TGGGCCTGAG 
AAAGCCAAGA GTCACGGAGT CTTGGCCGAC TTCTTCTCAG GGGCCTGGAG CCAGGGCATC 
AAGAAACTCA TCACCCTGCC ACTCGTGGGA AAACCCCTGA ACCTGGACCG CAAGGTGGCC 
CCTCAGCCCC TGTGGTTCTC CGACACGGTT GCAAACCTGA GGAAGCTGAA GGAGTTACCC 
TACCACTTAG TCCATGCGGG CCGTATAGAG GAGCTGAAGC AGGAGGTGCT GGGCAACATG 
ACCTGGATTT GCTGCCGGGG CCTCTCTGGG GGCATCGAGG GTCTGCTGGA AGACTTTGAC 
CTGTGTGCCC CGCACATGGA CTGTCCTGAG GTCAGCCTGG TCCGGGAAGC TCTCCAGCTG 
TGCCGCCCGG CAGTGGAGCT CCGAGGCATG GAGAGAAGCG TCCTGTGCAC AGAACTCCTG 
GCCAGACTCC ATTTCTTCAC CACTTCACAT CCCGGGCTGG TGGGACAGCT GTGCCAACAG 
GCACAGAGCT GGCTCCGGAT GTGCCCACAG CCTGTGCTGG TGCCCCTCGG AGGATTCCTC 
CCTCCGCCTG GAGGGCCCCT GCGGGCAACC CTCACCGGCT GTCACAAAGG CATCACTGCC 
ATGGCATGGA GCCTGGAAGA GAAGCTGCTG CTGGTTGGCA CGCAGGAGGG CATGGTGGCT 
GTGTGGGACA TGGAGGAACA GCAGGCAGTC CACATCCTAC CTGGACACAC AGGAGAGGTG 
AGGTGTGTGG AAGTGTTTGC CCAAGGGACA CTTGCCGTCT CTGCCTCGAA AGACCACACC 
TTGCGCTTGT GGGACTTACT CTCTGGCCAG GAGAAATTCA CCATTTGGGA TGGAGGTTCA 
AAAGATCCCA CTGAATCTCA GACCTGGAGC CTTCATGTGG ATGAAGCAAA GAAGGTTGTG 
TATTCAGCTT CAGGCTCAAA GGTCAGTGCT TGGAATCTGG AAACTGCAGA GCAGATTTTC 
CATATCCTGG GGGATGCCTC TGACCCCTGG GTATGCACGG CAGTGCTGGC TTCCCAGGCC 
ACCCTGCTGA CTGTGTCCCG GGGCGGCATG ATCAGTCTGT GGAGCTCAGC CACGGGAACA 
CTGCACAGGC GGCAACGTTT GTCCAGCATC AAAGAAGATA CACTTGCCTG CGGGGTCTCA 
GTCCAGAAAC AAAGAATGAT GGTTACTGGG TCCAGCACAG GCTCCATCTC TTTGGTTTCC 
TCCGAAGGAG ACAGCCTGCT GGAAAAGCTT CCGGAAGCTG TGGGGTTCCT GGTGGTATCT 
GAAGATGAGT CTCTTCTCGC TGCAGGTTTT GGAAGGTCTG TGAGGATATT TTTGGCAGAT 
GCACAGGGAT TTCATCGATT CATGGCCACT GACCTTGAAC ATGAAGATGT GGTAGAGACA 
GCCACTTTTG GTCCTGAAAA CAATCTGATT GTCACTGGGT CCCGGGATGC ACTCATTCAG 
GTGTGGAGTC TGTCAGCCCA GGGGACTCTG CTGGACGTCC TGGACGGTGT CGGGGCCCCG 
GTGAGCCTGC TGGCCTGGGG CGGGGCCTTG GTGGCATCTG CTTCCCGTCA GTCCTCATCT 
TTTAAAGTCT GGGATCTCAC CTGCACTCAG AAGCCGAGGG CCTCTAACCC ATTCCTGGAT 
CGCACTGGCC TCACGGCAGT CTCCCACAAC GGCAGCTACG TCTACTTCCC TAAAACTGGG 
GACAAAAACA AAGTCAGCAT TTGGGCCTTG GCAGAAGGTG AGGAGCAAGA TGCTCTGGAC 
ACCTCCAACG AGGTCAGATG TCTGGAGGTC GCTGAGCAGG CCAATCTCCT TTTCACTGGC 
CTCGTTTCAG GGGTTGTCCT GGTGTTCCCC CTGAATTCCA GACAAGACGT GATGTGTATC 
CCTCCTCCAG AAGCCCGGAA GGCCGTCAAC TGCATGGCTC TCAGTAAGGA CGAGGGGCAC 
CTGGCCATTG CCTATGACAG CACCGTCCTG GTGCTGGACA TCAGCCCGGG GGACCCCTGT 
CCAGTCATCG ATGGGCCAAA ATACACCTTC TATACCCAGC TGCCTGACAC CATAGCCAGT 
GTGGCTGCTC TGGCTGACTA TCGTGTCATC TACGGCATGC TCAACGGCGA CCTCTTCCTT 
TACGAGTGTG TGAATTCCAA GGTGTTCCCT CTGGAGGCCC ATGGGAGCCG AGTCACCTGT 
GTGGAGGTCA GCCACAAGGA GCAGCTGGCA GTCAGCGGGG CTGAGGAAGC CCTACTGTGT 
CTCTGGGACC TGCAGGCGTG CAAGTGGAAA TTCGAGATGA GCTACACGAA TTCTTACTGT 
GTAGGAGTCC AATGTGCTTG CTTCTCCAAG GATGACAAGT ATGTCTATGC AGGCTTGAAA 
GATCGCTCCA TAATTGTCTG GAGTGTGCTG GATGGCACAC TGTTGGCTGT CCAGTTTGTC 
CATGCCGTGG TGAACAGAAT CATCCCAACC TCCAATGGCT TCATTGCCCC CTCCAGACAC 
GGCTATCTCA TCCGTGAGCG TTTCCAGTGC CCTTCGTCAA GACTCTCACA ACAGGACCCT 
CTCAAGAACT TCAAGAAAGC AGTGTGGATG GTCAAATCCA GGCAGAGAGA AGAGCTGGCA 
GCTGCTGCAG GAGCACCCCA GGACTTGAGG TCAGAGAGTG CCCAGGGAAA TGAATCCAAA 
TCAAACAAAC GCTCACAAGT CTGCCTGCTG GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_012128876.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011984266.1 Ovis aries musimon NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv12828
Clone ID Related Accession (Same CDS sequence)	XM_012128876.2
Accession Version	XM_012128876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942348.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012128876.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAG AGAGGGAAGC CCTACAGAGC ACCGCCTACC CTGAAGTGCA GACCTTCTGC 
CAGAAGCACG GCCTGATGTT CGAGGTGGTT GACCTGCGGT GGGGTATTCG GAACACTGAG 
GCCACTAACC CCTTGACTAC AGAAATCTGC TTGGAGGAGC TGGACCGGTG TCGGGAAATA 
TCCATTGGGC CAGCCTTTGT TGCCCTCGTG GGTGACCAGT ACGGTCCCTG CCCAGTCCCC 
ACACGGATCG AGGAAAAGGA GTGGGAGGCG CTGAAGGCTC AGCTGACCAC CAGGCCCCGC 
GACCTGGAGC TGGTGGCACG AAGCTTCTGG AGGGACGAGA ACACGGTGCC CCCTGCCTAC 
CTGCTGCAGG CCCTGGGCAC TGGGGAGGCC TCTGGGCCTG AGGAGGCCAC CCTGATCTCT 
GCACTGCGCT CTGGAGCCCA GGAGGCCCAC AGACTGGGGC TTATCAGCCA GGAGCAGTGG 
CACCGCTACT ACCGGTCAGT CATCGAGTGG GAAATAGAAC GGGCCCTGCT GAGCTCAACG 
GTTGGGGATC ACGGGGCAAC AGTCTTCCTG AGAGATATCC AAGGCCTCAA TAAACACATC 
CTGGAAGACT GTGCCCTCAA AATGGTGGAC CGTCTTGCAG ATGGTTGCCT GGACACGGAT 
GCCCAGAACC TTCTCAGCAA CCTCAAGGGG CGCATTGCTG ACCGGCAGCC TGGAGTTCTC 
AAGGCCCACC ACCTAGCGTG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCC 
CGATACCTGA AGGAGCTGGG GGAGCAGTTT GTGGCCAGGG CCAACCATCA GGTTCTTGAG 
CGCCTCCGGG AGCTGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GACTCAGTGC CGAGGTCAAC CGGACCTTCT GTGGACGCCA GGAGCTCCTG 
ACCCAGCTGG GGCAGCGACT GCGACAGAGT GATGGCCGCC CCCACCCACC CCTGGTGCTT 
TTTGGACCCG CAGGCATCGG AAAGACGGCT CTGATGTGCA AGCTGGCTGA GCAGATGTCG 
GGGCTGCTCG GCCGCAAGAC AGTAACCATT CTGCGGCTTC TGGGGACATC TCAGATGAGC 
TCTGAGGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTATGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTAGAGGCC CACACCAGGC TGGTCCAGTT TTTCCACATC 
CTCCTCCACA CCGTCTCCAG CCGAAACTTT GAGTCCCTTG TGATCCTGCT GGATTCGGTG 
GATGAGGTGG ACTCTGTCCA CTGTGCTAGG AGGATCCCCT GGCTGCCTCC CAAATGTCCT 
CCAAGGGTCC ACCTCATCGT CTCGGCCTGC TCTGGACAGC GGGGGGCTTT AGACACCATG 
CGGCAGGTGC TCACGGACCA AGGGGCTTAC TGGGAGGTGA AACCCCTCTC TGGAAACCAA 
GGGCAAGAGA TGATCCAGCT CCTGCTGGCC GCCTCGAGGA GGACACTGAG TCAGGTGCAG 
AGGGACATGC TCTGGGCCAG CCTCCCAGAG TGTGGACACC CAGGGCGGCT GAGGCTGGCT 
TTCGAGGAGG CCCGGAAGTG GGCCTCCTTC ACTGTGCCAG CCCCACTGGC CTCCACTGCT 
AAGGAGGCGA TGCAACAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCG GCTCCTGGTG 
GCCCGCGTGC TGGGCTACAT TGTGTCTTCC CGGCATGGGC TCTCAGAGGC GGAGCTAAAG 
GATGTCCTGT CCTTGGATGA TGAGGTCCTG CAGAGCGTGT ACCAAGACTG GACCCCACCC 
AGCAAACAGC TTCTGCGCTT CCCGCCCCTG CTGTGGGTGA GGCTTCGTCG AGATCTGGGT 
AACTGCTTGG CCAGGCGGCC GGTGGACGGC TTCACCCTTC TGGCCATTGC CCACAGACAG 
CTGGCCGAGG TGGTCCGAGA GCTCTACCTG TCTGGGCCTG AGAAAGCCAA GAGTCACGGA 
GTCTTGGCCG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA TCAAGAAACT CATCACCCTG 
CCACTCGTGG GAAAACCCCT GAACCTGGAC CGCAAGGTGG CCCCTCAGCC CCTGTGGTTC 
TCCGACACGG TTGCAAACCT GAGGAAGCTG AAGGAGTTAC CCTACCACTT AGTCCATGCG 
GGCCGTATAG AGGAGCTGAA GCAGGAGGTG CTGGGCAACA TGACCTGGAT TTGCTGCCGG 
GGCCTCTCTG GGGGCATCGA GGGTCTGCTG GAAGACTTTG ACCTGTGTGC CCCGCACATG 
GACTGTCCTG AGGTCAGCCT GGTCCGGGAA GCTCTCCAGC TGTGCCGCCC GGCAGTGGAG 
CTCCGAGGCA TGGAGAGAAG CGTCCTGTGC ACAGAACTCC TGGCCAGACT CCATTTCTTC 
ACCACTTCAC ATCCCGGGCT GGTGGGACAG CTGTGCCAAC AGGCACAGAG CTGGCTCCGG 
ATGTGCCCAC AGCCTGTGCT GGTGCCCCTC GGAGGATTCC TCCCTCCGCC TGGAGGGCCC 
CTGCGGGCAA CCCTCACCGG CTGTCACAAA GGCATCACTG CCATGGCATG GAGCCTGGAA 
GAGAAGCTGC TGCTGGTTGG CACGCAGGAG GGCATGGTGG CTGTGTGGGA CATGGAGGAA 
CAGCAGGCAG TCCACATCCT ACCTGGACAC ACAGGAGAGG TGAGGTGTGT GGAAGTGTTT 
GCCCAAGGGA CACTTGCCGT CTCTGCCTCG AAAGACCACA CCTTGCGCTT GTGGGACTTA 
CTCTCTGGCC AGGAGAAATT CACCATTTGG GATGGAGGTT CAAAAGATCC CACTGAATCT 
CAGACCTGGA GCCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTCAGC TTCAGGCTCA 
AAGGTCAGTG CTTGGAATCT GGAAACTGCA GAGCAGATTT TCCATATCCT GGGGGATGCC 
TCTGACCCCT GGGTATGCAC GGCAGTGCTG GCTTCCCAGG CCACCCTGCT GACTGTGTCC 
CGGGGCGGCA TGATCAGTCT GTGGAGCTCA GCCACGGGAA CACTGCACAG GCGGCAACGT 
TTGTCCAGCA TCAAAGAAGA TACACTTGCC TGCGGGGTCT CAGTCCAGAA ACAAAGAATG 
ATGGTTACTG GGTCCAGCAC AGGCTCCATC TCTTTGGTTT CCTCCGAAGG AGACAGCCTG 
CTGGAAAAGC TTCCGGAAGC TGTGGGGTTC CTGGTGGTAT CTGAAGATGA GTCTCTTCTC 
GCTGCAGGTT TTGGAAGGTC TGTGAGGATA TTTTTGGCAG ATGCACAGGG ATTTCATCGA 
TTCATGGCCA CTGACCTTGA ACATGAAGAT GTGGTAGAGA CAGCCACTTT TGGTCCTGAA 
AACAATCTGA TTGTCACTGG GTCCCGGGAT GCACTCATTC AGGTGTGGAG TCTGTCAGCC 
CAGGGGACTC TGCTGGACGT CCTGGACGGT GTCGGGGCCC CGGTGAGCCT GCTGGCCTGG 
GGCGGGGCCT TGGTGGCATC TGCTTCCCGT CAGTCCTCAT CTTTTAAAGT CTGGGATCTC 
ACCTGCACTC AGAAGCCGAG GGCCTCTAAC CCATTCCTGG ATCGCACTGG CCTCACGGCA 
GTCTCCCACA ACGGCAGCTA CGTCTACTTC CCTAAAACTG GGGACAAAAA CAAAGTCAGC 
ATTTGGGCCT TGGCAGAAGG TGAGGAGCAA GATGCTCTGG ACACCTCCAA CGAGGTCAGA 
TGTCTGGAGG TCGCTGAGCA GGCCAATCTC CTTTTCACTG GCCTCGTTTC AGGGGTTGTC 
CTGGTGTTCC CCCTGAATTC CAGACAAGAC GTGATGTGTA TCCCTCCTCC AGAAGCCCGG 
AAGGCCGTCA ACTGCATGGC TCTCAGTAAG GACGAGGGGC ACCTGGCCAT TGCCTATGAC 
AGCACCGTCC TGGTGCTGGA CATCAGCCCG GGGGACCCCT GTCCAGTCAT CGATGGGCCA 
AAATACACCT TCTATACCCA GCTGCCTGAC ACCATAGCCA GTGTGGCTGC TCTGGCTGAC 
TATCGTGTCA TCTACGGCAT GCTCAACGGC GACCTCTTCC TTTACGAGTG TGTGAATTCC 
AAGGTGTTCC CTCTGGAGGC CCATGGGAGC CGAGTCACCT GTGTGGAGGT CAGCCACAAG 
GAGCAGCTGG CAGTCAGCGG GGCTGAGGAA GCCCTACTGT GTCTCTGGGA CCTGCAGGCG 
TGCAAGTGGA AATTCGAGAT GAGCTACACG AATTCTTACT GTGTAGGAGT CCAATGTGCT 
TGCTTCTCCA AGGATGACAA GTATGTCTAT GCAGGCTTGA AAGATCGCTC CATAATTGTC 
TGGAGTGTGC TGGATGGCAC ACTGTTGGCT GTCCAGTTTG TCCATGCCGT GGTGAACAGA 
ATCATCCCAA CCTCCAATGG CTTCATTGCC CCCTCCAGAC ACGGCTATCT CATCCGTGAG 
CGTTTCCAGT GCCCTTCGTC AAGACTCTCA CAACAGGACC CTCTCAAGAA CTTCAAGAAA 
GCAGTGTGGA TGGTCAAATC CAGGCAGAGA GAAGAGCTGG CAGCTGCTGC AGGAGCACCC 
CAGGACTTGA GGTCAGAGAG TGCCCAGGGA AATGAATCCA AATCAAACAA ACGCTCACAA 
GTCTGCCTGC TGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011984266.1
CDS	615..5312
Translation	MDAEREALQSTAYPEVQTFCQKHGLMFEVVDLRWGIRNTEATNPLTTEICLEELDRCREISIGPAFVALVGDQYGPCPVPTRIEEKEWEALKAQLTTRPRDLELVARSFWRDENTVPPAYLLQALGTGEASGPEEATLISALRSGAQEAHRLGLISQEQWHRYYRSVIEWEIERALLSSTVGDHGATVFLRDIQGLNKHILEDCALKMVDRLADGCLDTDAQNLLSNLKGRIADRQPGVLKAHHLAWSRDLVNPKNKAHARYLKELGEQFVARANHQVLERLRELEAGRQELAWLYQEIRHHLGLSAEVNRTFCGRQELLTQLGQRLRQSDGRPHPPLVLFGPAGIGKTALMCKLAEQMSGLLGRKTVTILRLLGTSQMSSEARGLLQSICFQVCLAYGLPLPPAQVLEAHTRLVQFFHILLHTVSSRNFESLVILLDSVDEVDSVHCARRIPWLPPKCPPRVHLIVSACSGQRGALDTMRQVLTDQGAYWEVKPLSGNQGQEMIQLLLAASRRTLSQVQRDMLWASLPECGHPGRLRLAFEEARKWASFTVPAPLASTAKEAMQQLCARLEQTHGRLLVARVLGYIVSSRHGLSEAELKDVLSLDDEVLQSVYQDWTPPSKQLLRFPPLLWVRLRRDLGNCLARRPVDGFTLLAIAHRQLAEVVRELYLSGPEKAKSHGVLADFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPYHLVHAGRIEELKQEVLGNMTWICCRGLSGGIEGLLEDFDLCAPHMDCPEVSLVREALQLCRPAVELRGMERSVLCTELLARLHFFTTSHPGLVGQLCQQAQSWLRMCPQPVLVPLGGFLPPPGGPLRATLTGCHKGITAMAWSLEEKLLLVGTQEGMVAVWDMEEQQAVHILPGHTGEVRCVEVFAQGTLAVSASKDHTLRLWDLLSGQEKFTIWDGGSKDPTESQTWSLHVDEAKKVVYSASGSKVSAWNLETAEQIFHILGDASDPWVCTAVLASQATLLTVSRGGMISLWSSATGTLHRRQRLSSIKEDTLACGVSVQKQRMMVTGSSTGSISLVSSEGDSLLEKLPEAVGFLVVSEDESLLAAGFGRSVRIFLADAQGFHRFMATDLEHEDVVETATFGPENNLIVTGSRDALIQVWSLSAQGTLLDVLDGVGAPVSLLAWGGALVASASRQSSSFKVWDLTCTQKPRASNPFLDRTGLTAVSHNGSYVYFPKTGDKNKVSIWALAEGEEQDALDTSNEVRCLEVAEQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAVNCMALSKDEGHLAIAYDSTVLVLDISPGDPCPVIDGPKYTFYTQLPDTIASVAALADYRVIYGMLNGDLFLYECVNSKVFPLEAHGSRVTCVEVSHKEQLAVSGAEEALLCLWDLQACKWKFEMSYTNSYCVGVQCACFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPSRHGYLIRERFQCPSSRLSQQDPLKNFKKAVWMVKSRQREELAAAAGAPQDLRSESAQGNESKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_012128876.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012128876.2

ORF Insert Sequence:

ATGGATGCAG AGAGGGAAGC CCTACAGAGC ACCGCCTACC CTGAAGTGCA GACCTTCTGC 
CAGAAGCACG GCCTGATGTT CGAGGTGGTT GACCTGCGGT GGGGTATTCG GAACACTGAG 
GCCACTAACC CCTTGACTAC AGAAATCTGC TTGGAGGAGC TGGACCGGTG TCGGGAAATA 
TCCATTGGGC CAGCCTTTGT TGCCCTCGTG GGTGACCAGT ACGGTCCCTG CCCAGTCCCC 
ACACGGATCG AGGAAAAGGA GTGGGAGGCG CTGAAGGCTC AGCTGACCAC CAGGCCCCGC 
GACCTGGAGC TGGTGGCACG AAGCTTCTGG AGGGACGAGA ACACGGTGCC CCCTGCCTAC 
CTGCTGCAGG CCCTGGGCAC TGGGGAGGCC TCTGGGCCTG AGGAGGCCAC CCTGATCTCT 
GCACTGCGCT CTGGAGCCCA GGAGGCCCAC AGACTGGGGC TTATCAGCCA GGAGCAGTGG 
CACCGCTACT ACCGGTCAGT CATCGAGTGG GAAATAGAAC GGGCCCTGCT GAGCTCAACG 
GTTGGGGATC ACGGGGCAAC AGTCTTCCTG AGAGATATCC AAGGCCTCAA TAAACACATC 
CTGGAAGACT GTGCCCTCAA AATGGTGGAC CGTCTTGCAG ATGGTTGCCT GGACACGGAT 
GCCCAGAACC TTCTCAGCAA CCTCAAGGGG CGCATTGCTG ACCGGCAGCC TGGAGTTCTC 
AAGGCCCACC ACCTAGCGTG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCC 
CGATACCTGA AGGAGCTGGG GGAGCAGTTT GTGGCCAGGG CCAACCATCA GGTTCTTGAG 
CGCCTCCGGG AGCTGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GACTCAGTGC CGAGGTCAAC CGGACCTTCT GTGGACGCCA GGAGCTCCTG 
ACCCAGCTGG GGCAGCGACT GCGACAGAGT GATGGCCGCC CCCACCCACC CCTGGTGCTT 
TTTGGACCCG CAGGCATCGG AAAGACGGCT CTGATGTGCA AGCTGGCTGA GCAGATGTCG 
GGGCTGCTCG GCCGCAAGAC AGTAACCATT CTGCGGCTTC TGGGGACATC TCAGATGAGC 
TCTGAGGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTATGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTAGAGGCC CACACCAGGC TGGTCCAGTT TTTCCACATC 
CTCCTCCACA CCGTCTCCAG CCGAAACTTT GAGTCCCTTG TGATCCTGCT GGATTCGGTG 
GATGAGGTGG ACTCTGTCCA CTGTGCTAGG AGGATCCCCT GGCTGCCTCC CAAATGTCCT 
CCAAGGGTCC ACCTCATCGT CTCGGCCTGC TCTGGACAGC GGGGGGCTTT AGACACCATG 
CGGCAGGTGC TCACGGACCA AGGGGCTTAC TGGGAGGTGA AACCCCTCTC TGGAAACCAA 
GGGCAAGAGA TGATCCAGCT CCTGCTGGCC GCCTCGAGGA GGACACTGAG TCAGGTGCAG 
AGGGACATGC TCTGGGCCAG CCTCCCAGAG TGTGGACACC CAGGGCGGCT GAGGCTGGCT 
TTCGAGGAGG CCCGGAAGTG GGCCTCCTTC ACTGTGCCAG CCCCACTGGC CTCCACTGCT 
AAGGAGGCGA TGCAACAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCG GCTCCTGGTG 
GCCCGCGTGC TGGGCTACAT TGTGTCTTCC CGGCATGGGC TCTCAGAGGC GGAGCTAAAG 
GATGTCCTGT CCTTGGATGA TGAGGTCCTG CAGAGCGTGT ACCAAGACTG GACCCCACCC 
AGCAAACAGC TTCTGCGCTT CCCGCCCCTG CTGTGGGTGA GGCTTCGTCG AGATCTGGGT 
AACTGCTTGG CCAGGCGGCC GGTGGACGGC TTCACCCTTC TGGCCATTGC CCACAGACAG 
CTGGCCGAGG TGGTCCGAGA GCTCTACCTG TCTGGGCCTG AGAAAGCCAA GAGTCACGGA 
GTCTTGGCCG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA TCAAGAAACT CATCACCCTG 
CCACTCGTGG GAAAACCCCT GAACCTGGAC CGCAAGGTGG CCCCTCAGCC CCTGTGGTTC 
TCCGACACGG TTGCAAACCT GAGGAAGCTG AAGGAGTTAC CCTACCACTT AGTCCATGCG 
GGCCGTATAG AGGAGCTGAA GCAGGAGGTG CTGGGCAACA TGACCTGGAT TTGCTGCCGG 
GGCCTCTCTG GGGGCATCGA GGGTCTGCTG GAAGACTTTG ACCTGTGTGC CCCGCACATG 
GACTGTCCTG AGGTCAGCCT GGTCCGGGAA GCTCTCCAGC TGTGCCGCCC GGCAGTGGAG 
CTCCGAGGCA TGGAGAGAAG CGTCCTGTGC ACAGAACTCC TGGCCAGACT CCATTTCTTC 
ACCACTTCAC ATCCCGGGCT GGTGGGACAG CTGTGCCAAC AGGCACAGAG CTGGCTCCGG 
ATGTGCCCAC AGCCTGTGCT GGTGCCCCTC GGAGGATTCC TCCCTCCGCC TGGAGGGCCC 
CTGCGGGCAA CCCTCACCGG CTGTCACAAA GGCATCACTG CCATGGCATG GAGCCTGGAA 
GAGAAGCTGC TGCTGGTTGG CACGCAGGAG GGCATGGTGG CTGTGTGGGA CATGGAGGAA 
CAGCAGGCAG TCCACATCCT ACCTGGACAC ACAGGAGAGG TGAGGTGTGT GGAAGTGTTT 
GCCCAAGGGA CACTTGCCGT CTCTGCCTCG AAAGACCACA CCTTGCGCTT GTGGGACTTA 
CTCTCTGGCC AGGAGAAATT CACCATTTGG GATGGAGGTT CAAAAGATCC CACTGAATCT 
CAGACCTGGA GCCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTCAGC TTCAGGCTCA 
AAGGTCAGTG CTTGGAATCT GGAAACTGCA GAGCAGATTT TCCATATCCT GGGGGATGCC 
TCTGACCCCT GGGTATGCAC GGCAGTGCTG GCTTCCCAGG CCACCCTGCT GACTGTGTCC 
CGGGGCGGCA TGATCAGTCT GTGGAGCTCA GCCACGGGAA CACTGCACAG GCGGCAACGT 
TTGTCCAGCA TCAAAGAAGA TACACTTGCC TGCGGGGTCT CAGTCCAGAA ACAAAGAATG 
ATGGTTACTG GGTCCAGCAC AGGCTCCATC TCTTTGGTTT CCTCCGAAGG AGACAGCCTG 
CTGGAAAAGC TTCCGGAAGC TGTGGGGTTC CTGGTGGTAT CTGAAGATGA GTCTCTTCTC 
GCTGCAGGTT TTGGAAGGTC TGTGAGGATA TTTTTGGCAG ATGCACAGGG ATTTCATCGA 
TTCATGGCCA CTGACCTTGA ACATGAAGAT GTGGTAGAGA CAGCCACTTT TGGTCCTGAA 
AACAATCTGA TTGTCACTGG GTCCCGGGAT GCACTCATTC AGGTGTGGAG TCTGTCAGCC 
CAGGGGACTC TGCTGGACGT CCTGGACGGT GTCGGGGCCC CGGTGAGCCT GCTGGCCTGG 
GGCGGGGCCT TGGTGGCATC TGCTTCCCGT CAGTCCTCAT CTTTTAAAGT CTGGGATCTC 
ACCTGCACTC AGAAGCCGAG GGCCTCTAAC CCATTCCTGG ATCGCACTGG CCTCACGGCA 
GTCTCCCACA ACGGCAGCTA CGTCTACTTC CCTAAAACTG GGGACAAAAA CAAAGTCAGC 
ATTTGGGCCT TGGCAGAAGG TGAGGAGCAA GATGCTCTGG ACACCTCCAA CGAGGTCAGA 
TGTCTGGAGG TCGCTGAGCA GGCCAATCTC CTTTTCACTG GCCTCGTTTC AGGGGTTGTC 
CTGGTGTTCC CCCTGAATTC CAGACAAGAC GTGATGTGTA TCCCTCCTCC AGAAGCCCGG 
AAGGCCGTCA ACTGCATGGC TCTCAGTAAG GACGAGGGGC ACCTGGCCAT TGCCTATGAC 
AGCACCGTCC TGGTGCTGGA CATCAGCCCG GGGGACCCCT GTCCAGTCAT CGATGGGCCA 
AAATACACCT TCTATACCCA GCTGCCTGAC ACCATAGCCA GTGTGGCTGC TCTGGCTGAC 
TATCGTGTCA TCTACGGCAT GCTCAACGGC GACCTCTTCC TTTACGAGTG TGTGAATTCC 
AAGGTGTTCC CTCTGGAGGC CCATGGGAGC CGAGTCACCT GTGTGGAGGT CAGCCACAAG 
GAGCAGCTGG CAGTCAGCGG GGCTGAGGAA GCCCTACTGT GTCTCTGGGA CCTGCAGGCG 
TGCAAGTGGA AATTCGAGAT GAGCTACACG AATTCTTACT GTGTAGGAGT CCAATGTGCT 
TGCTTCTCCA AGGATGACAA GTATGTCTAT GCAGGCTTGA AAGATCGCTC CATAATTGTC 
TGGAGTGTGC TGGATGGCAC ACTGTTGGCT GTCCAGTTTG TCCATGCCGT GGTGAACAGA 
ATCATCCCAA CCTCCAATGG CTTCATTGCC CCCTCCAGAC ACGGCTATCT CATCCGTGAG 
CGTTTCCAGT GCCCTTCGTC AAGACTCTCA CAACAGGACC CTCTCAAGAA CTTCAAGAAA 
GCAGTGTGGA TGGTCAAATC CAGGCAGAGA GAAGAGCTGG CAGCTGCTGC AGGAGCACCC 
CAGGACTTGA GGTCAGAGAG TGCCCAGGGA AATGAATCCA AATCAAACAA ACGCTCACAA 
GTCTGCCTGC TGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_012177722.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012033112.2 Ovis aries NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

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CloneID	OSm49596
Clone ID Related Accession (Same CDS sequence)	XM_012177722.2
Accession Version	XM_012177722.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	LOW QUALITY PROTEIN: NACHT domain- and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639014.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012177722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCAGAGAG GGAAGCCCTA CAGAGCACCG CCTACCCTGA AGTGCNAGAC ATTCTGCCAG 
AAGCACGGCC TGATGTTCGA GGTGGTTGAC CTGTGGTGGG GTATTCGGAA CACTGAGGCC 
ACTAACCCCC TGACTACAGA AATCTGCTTG GAGGAGCTGG ACCGGTGTCG GGAAATATCC 
ATTGGGCCAG CCTTTGTTGC CCTCGTGGGT GACCAGTACG GTCCCTGCCC AGTCCCCACG 
CGGATCGAGG AAAAGGAGTG GGAGGCATTG AAGGCTCAGC TGACTGCCAG GCCCCACGAC 
CTGGAGCTGG TGGCACGAAG CTTCTGGAGG GACGAGAATG CGGTGCCCCC CGCCTACCTG 
CTGCAGGCCC TGGGCACTGG GGAGGCCTCT GGGCCTGAGG AGGCCACCCT GATCTCTGCA 
CTGCGCTCCG GAGCCCAGGA GGCCCACAGA CTGGGGCTCA TCAGCCAGGA GCAGTGGCAC 
CACTACTACC GGTCAGTCAT CGAGTGGGAA ATAGAACGGG CCCTGCTGAG CTCAACGGTT 
GGGGATCACG GGGCAACAGT CTTCCTGAGA GATATCCAAG GCCTCAATAA ACACATCCTG 
GAAGACTGTG CCCTCAAAAT GGTGGACCGT CTTGCAGATG GTTGCCTGGA CACGGATGCC 
CAGAACCTTC TCAGCAACCT CAAGGGGCGC ATTGCTGACC GGCAGCCTGG AGTTCTCAAG 
GCCCACCACC TAGCGTGGAG CCGAGACCTG GTGAACCCCA AAAACAAGGC TCATGCCCGT 
TACCTGAAGG AGCTGGGGGA GCAGTTTGTG GCCAGGGCCA ACCATCAGGT TCTTGAGCGC 
CTCCGGGAGC TGGAGGCAGG CAGGCAGGAG TTGGCGTGGC TCTACCAGGA GATCCGCCAC 
CACCTGGGAC TCAGTGCCGA GGTCAACCGG ACCTTCTGTG GACGCCAGGA GCTCCTGACC 
CAGCTGGGGC AGCGACTGCG ACAGAGTGAT GGCCGCCCCC ACCCACCCCT GGTGCTTTTT 
GGACCCGCAG GCATCGGAAA GACGGCTCTG ATGTGCAAGC TGGCTGAGCA GATGTCGGGG 
CTGCTCGGCC GCAAGACAGT AACCATTCTG CGGCTTCTGG GGACATCTCA GATGAGCTCT 
GAGGCCCGAG GCCTGCTCCA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
CTGCCCCCTG CCCAGGTCCT AGAGGCCCAC ACCAGGCTGG TCCAGTTTTT CCACATCCTC 
CTCCACACCG TCTCCAGCCG AAACTTTGAG TCCCTTGTGA TCCTGCTGGA TTCGGTGGAT 
GAGGTGGACT CTGTCCACTG TGCTAGGAGG ATCCCCTGGC TGCCTCCCAA ATGTCCTCCA 
AGGGTCCACC TCATCGTCTC GGCCTGCTCT GGACAGCGGG GGGCTTTAGA CACCATGCGG 
CAGGTGCTCA CGGACCAAGG GGCTTACTGG GAGGTGAAAC CCCTCTCTGG AAACCAAGGG 
CAAGAGATGA TCCAGCTCCT GCTGGCCGCC TCGAGGAGGA CACTGAGTCA GGTGCAGAGG 
GACATGCTCT GGGCCAGCCT CCCAGAGTGT GGACACCCAG GGCGGCTGAG GCTGGCTTTC 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGCCC CACTGGCCTC CACTGCTAAG 
GAGGCGATGC AACAGCTGTG TGCCCGCCTG GAGCAGACAC ACGGGCGGCT CCTGGTGGCC 
CGCGTGCTGG GCTACATTGT GTCTTCCCGG CATGGGCTCT CAGAGGCGGA GCTAAAGGAT 
GTCCTGTCCT TGGATGACGA GGTCCTGCAG AGCGTGTACC AAGACTGGAC CCCACCCAGC 
AAACAGCTTC TGCGCTTCCC GCCCCTGCTG TGGGTGAGGC TTCGTCGAGA TCTGGGTAAC 
TGCTTGGCCA GGCGGCCGGT GGACGGCTTC ACCCTTCTGG CCATTGCCCA CAGACAGCTG 
GCCGAGGTGG TCCGAGAGCT CTACCTGTCT GGGCCTGAGA AAGCCAAGAG TCACGGAGTC 
TTGGCCGACT TCTTCTCAGG GGCCTGGAGC CAGGGCATCA AGAAACTCAT CACCCTGCCA 
CTCGTGGGAA AACCCCTGAA CCTGGACCGC AAGGTGGCCC CTCAGCCCCT GTGGTTCTCC 
GACACGGTTG CAAACCTGAG GAAGCTGAAG GAGTTACCCT ACCACTTAGT CCATGCGGGC 
CGTATAGAGG AGCTGAAGCA GGAGGTGCTG GGCTGCATGA CCTGGATTTG CTGCCGGGGC 
CTCTCTGGGG GCATCGAGGG TCTGCTGGAA GACTTTGACC TGTGTGCCCC GCACATGGAC 
TGTCCTGAGG TCAGCCTGGT CCGGGAAGCT CTCCACCTGT GCCGCCCGGC AGTGGAGCTC 
CGAGGCATGG AGAGAAGCGT CCTGTGCACA GAACTCCTGG CCAGACTCCA TTTCTTCACC 
ACTTCACATC CCGGGCTGGT GGGACAGCTG TGCCAACAGG CAGAGAGCTG GCTCCGGATG 
TGCCCACAGC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC CTCCGCCTGG AGGGCCCCTG 
CGGGCAACCC TCACCGGCTG TCACAAAGGC ATCACTGCCA TGGCATGGAG CCTGGAAGAG 
AAGCTGCTGC TGGTTGGCAC GCAGGAGGGC ATGGTGGCTG TGTGGGACAT GGAGGAACAG 
CAGGCAGTCC ACATCCTACC TGGACACACA GGAGAGGTGA GGTGTGTGGA AGTGTTTGCC 
CAAGGGACAC TTGCCGTCTC TGCCTCGAAA GACCACACCT TGCGCTTGTG GGACTTACTC 
TCTGGCCAGG AGAAATTCAC CATTTGGGAT GGAGGTTCAA AAGATCCCAC TGAATCTCAG 
ACCTGGAGCC TTCATGTGGA TGAAGCAAAG AAGGTTGTGT ATTCAGCTTC AGGCTCAAAG 
GTCAGTGCTT GGAATCTGGA AACTGCAGAG CAGATTTTCC ATATCCTGGG GGATGCCTCT 
GACCCCTGGG TATGCACGGC AGTGCTGGCT TCCCAGGCCA CCCTGCTGAC TGTGTCCCGG 
GGCGGCATGA TCAGTCTGTG GAGCTCAGCC ACGGGAACAC TGCACAGGCG GCAACGTTTG 
TCCAGCATCA AAGAAGATAC ACTTGCCTGC GGGGTCTCAG TCCAGAAACA AAGAATGATG 
GTTACTGGGT CCAGCACAGG CTCCATCTCT TTGGTTTCCT CCGAAGGAGA CAGCCTGCTG 
GAAAAGCTTC CGGAAGCTGT GGGGTTCCTG GTGGTATCTG AAGATGAGTC TCTTCTCGCT 
GCAGGTTTTG GAAGGTCTGT GAGGATATTT TTGGCAGATG CACAGGGATT TCATCGATTC 
ATGGCCACTG ACCTTGAACA TGAAGATGTG GTAGAGACAG CCACTTTTGG TCCTGAAAAC 
AATCTGATTG TCACTGGGTC CCGGGATGCA CTCATTCAGG TGTGGAGTCT GTCAGCCCAG 
GGGACTCTGC TGGACGTCCT GGACGGTGTC GGGGCCCCGG TGAGCCTGCT GGCCCGGGGC 
GGGGCCTTGG TGGCATCTGC TTCCCGTCAG TCCTCATCTT TTAAAGTCTG GGATCTCACC 
TGCGCTCAGA AGCCGAGGGC CTCTAACCCA TTCCTGGATC GCACTGGCCT CACGGCAGTC 
TCCCACAACG GCAGCTACGT CTACTTCCCT AAAACTGGGG ACAAAAACAA AGTCAGCATT 
TGGGCCTTGG CAGAAGGTGA GGAGCAAGAT GCTCTGGACA CCTCCAACGA GGTCAGATGT 
CTGGAGGTCG CTGAGCAGGC CAATCTCCTT TTCACTGGCC TCGTTTCAGG GGTTGTCCTG 
GTGTTCCCCC TGAATTCCAG ACAAGACGTG ATGTGTATCC CTCCTCCAGA AGCCCGGAAG 
GCCGTCAACT GCATGGCTCT CAGTAAGGAC GAGGGGCACC TGGCCATTGC CTATGACAGC 
ACCGTCTTGG TGCTGGACAT CAGCCCGGGG GACCCCTGTC CAGTCATCGA TGGGCCAAAA 
TACACCTTCT ATACCCAGCT GCCTGACACC ATAGCCAGTG TGGCTGCTCT GGCTGACTAT 
CGTGTCATCT ACGGCATGCT CAACGGCGAC CTCTTCCTTT ACGAGTGTGT GAATTCCAAG 
GTGTTCCCTC TGGAGGCCCA TGGGAGCCGA GTCACCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGCAG TCAGCGGGGC TGAGGAAGCC CTACTGTGTC TCTGGGACCT GCAGGCGTGC 
AAGTGGAAAT TCGAGATGAG CTACACGAAT TCTTACTGTG TAGGAGTCCA ATGTGCTTGC 
TTCTCCAAGG ATGACAAGTA TGTCTATGCA GGCTTGAAAG ATCGCTCCAT AATTGTCTGG 
AGTGTGCTGG ATGGCACACT GTTGGCTGTC CAGTTTGTCC ATGCCGTGGT GAACAGAATC 
ATCCCAACCT CCAATGGCTT CATTGCCCCC TCCAGACACG GCTATCTCAT CCGTGAGCGT 
TTCCAGTGCC CTTCGTCAAG ACTCTCACAA CAGGACCCTC TCAAGAACTT CAAGAAAGCA 
GTGTGGATGG TCAAATCCAG GCAGAGAGAA GAGCTGGCAG CTGCTGCAGG AGCACCCCAG 
GACTTGAGGT CAGAGAGTGC CCAGGGAAAT GAATCCAAAT CAAACAAACG CTCACAAGTC 
TGCCTGCTGG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012033112.2
CDS	585..5279
Translation	MQRGKPYRAPPTLKCnullTFCQKHGLMFEVVDLWWGIRNTEATNPLTTEICLEELDRCREISIGPAFVALVGDQYGPCPVPTRIEEKEWEALKAQLTARPHDLELVARSFWRDENAVPPAYLLQALGTGEASGPEEATLISALRSGAQEAHRLGLISQEQWHHYYRSVIEWEIERALLSSTVGDHGATVFLRDIQGLNKHILEDCALKMVDRLADGCLDTDAQNLLSNLKGRIADRQPGVLKAHHLAWSRDLVNPKNKAHARYLKELGEQFVARANHQVLERLRELEAGRQELAWLYQEIRHHLGLSAEVNRTFCGRQELLTQLGQRLRQSDGRPHPPLVLFGPAGIGKTALMCKLAEQMSGLLGRKTVTILRLLGTSQMSSEARGLLQSICFQVCLAYGLPLPPAQVLEAHTRLVQFFHILLHTVSSRNFESLVILLDSVDEVDSVHCARRIPWLPPKCPPRVHLIVSACSGQRGALDTMRQVLTDQGAYWEVKPLSGNQGQEMIQLLLAASRRTLSQVQRDMLWASLPECGHPGRLRLAFEEARKWASFTVPAPLASTAKEAMQQLCARLEQTHGRLLVARVLGYIVSSRHGLSEAELKDVLSLDDEVLQSVYQDWTPPSKQLLRFPPLLWVRLRRDLGNCLARRPVDGFTLLAIAHRQLAEVVRELYLSGPEKAKSHGVLADFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPYHLVHAGRIEELKQEVLGCMTWICCRGLSGGIEGLLEDFDLCAPHMDCPEVSLVREALHLCRPAVELRGMERSVLCTELLARLHFFTTSHPGLVGQLCQQAESWLRMCPQPVLVPLGGFLPPPGGPLRATLTGCHKGITAMAWSLEEKLLLVGTQEGMVAVWDMEEQQAVHILPGHTGEVRCVEVFAQGTLAVSASKDHTLRLWDLLSGQEKFTIWDGGSKDPTESQTWSLHVDEAKKVVYSASGSKVSAWNLETAEQIFHILGDASDPWVCTAVLASQATLLTVSRGGMISLWSSATGTLHRRQRLSSIKEDTLACGVSVQKQRMMVTGSSTGSISLVSSEGDSLLEKLPEAVGFLVVSEDESLLAAGFGRSVRIFLADAQGFHRFMATDLEHEDVVETATFGPENNLIVTGSRDALIQVWSLSAQGTLLDVLDGVGAPVSLLARGGALVASASRQSSSFKVWDLTCAQKPRASNPFLDRTGLTAVSHNGSYVYFPKTGDKNKVSIWALAEGEEQDALDTSNEVRCLEVAEQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAVNCMALSKDEGHLAIAYDSTVLVLDISPGDPCPVIDGPKYTFYTQLPDTIASVAALADYRVIYGMLNGDLFLYECVNSKVFPLEAHGSRVTCVEVSHKEQLAVSGAEEALLCLWDLQACKWKFEMSYTNSYCVGVQCACFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPSRHGYLIRERFQCPSSRLSQQDPLKNFKKAVWMVKSRQREELAAAAGAPQDLRSESAQGNESKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_012177722.2
Organism	Ovis aries(sheep)
Definition	Ovis aries NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012177722.2

ORF Insert Sequence:

ATGCAGAGAG GGAAGCCCTA CAGAGCACCG CCTACCCTGA AGTGCNAGAC ATTCTGCCAG 
AAGCACGGCC TGATGTTCGA GGTGGTTGAC CTGTGGTGGG GTATTCGGAA CACTGAGGCC 
ACTAACCCCC TGACTACAGA AATCTGCTTG GAGGAGCTGG ACCGGTGTCG GGAAATATCC 
ATTGGGCCAG CCTTTGTTGC CCTCGTGGGT GACCAGTACG GTCCCTGCCC AGTCCCCACG 
CGGATCGAGG AAAAGGAGTG GGAGGCATTG AAGGCTCAGC TGACTGCCAG GCCCCACGAC 
CTGGAGCTGG TGGCACGAAG CTTCTGGAGG GACGAGAATG CGGTGCCCCC CGCCTACCTG 
CTGCAGGCCC TGGGCACTGG GGAGGCCTCT GGGCCTGAGG AGGCCACCCT GATCTCTGCA 
CTGCGCTCCG GAGCCCAGGA GGCCCACAGA CTGGGGCTCA TCAGCCAGGA GCAGTGGCAC 
CACTACTACC GGTCAGTCAT CGAGTGGGAA ATAGAACGGG CCCTGCTGAG CTCAACGGTT 
GGGGATCACG GGGCAACAGT CTTCCTGAGA GATATCCAAG GCCTCAATAA ACACATCCTG 
GAAGACTGTG CCCTCAAAAT GGTGGACCGT CTTGCAGATG GTTGCCTGGA CACGGATGCC 
CAGAACCTTC TCAGCAACCT CAAGGGGCGC ATTGCTGACC GGCAGCCTGG AGTTCTCAAG 
GCCCACCACC TAGCGTGGAG CCGAGACCTG GTGAACCCCA AAAACAAGGC TCATGCCCGT 
TACCTGAAGG AGCTGGGGGA GCAGTTTGTG GCCAGGGCCA ACCATCAGGT TCTTGAGCGC 
CTCCGGGAGC TGGAGGCAGG CAGGCAGGAG TTGGCGTGGC TCTACCAGGA GATCCGCCAC 
CACCTGGGAC TCAGTGCCGA GGTCAACCGG ACCTTCTGTG GACGCCAGGA GCTCCTGACC 
CAGCTGGGGC AGCGACTGCG ACAGAGTGAT GGCCGCCCCC ACCCACCCCT GGTGCTTTTT 
GGACCCGCAG GCATCGGAAA GACGGCTCTG ATGTGCAAGC TGGCTGAGCA GATGTCGGGG 
CTGCTCGGCC GCAAGACAGT AACCATTCTG CGGCTTCTGG GGACATCTCA GATGAGCTCT 
GAGGCCCGAG GCCTGCTCCA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
CTGCCCCCTG CCCAGGTCCT AGAGGCCCAC ACCAGGCTGG TCCAGTTTTT CCACATCCTC 
CTCCACACCG TCTCCAGCCG AAACTTTGAG TCCCTTGTGA TCCTGCTGGA TTCGGTGGAT 
GAGGTGGACT CTGTCCACTG TGCTAGGAGG ATCCCCTGGC TGCCTCCCAA ATGTCCTCCA 
AGGGTCCACC TCATCGTCTC GGCCTGCTCT GGACAGCGGG GGGCTTTAGA CACCATGCGG 
CAGGTGCTCA CGGACCAAGG GGCTTACTGG GAGGTGAAAC CCCTCTCTGG AAACCAAGGG 
CAAGAGATGA TCCAGCTCCT GCTGGCCGCC TCGAGGAGGA CACTGAGTCA GGTGCAGAGG 
GACATGCTCT GGGCCAGCCT CCCAGAGTGT GGACACCCAG GGCGGCTGAG GCTGGCTTTC 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGCCC CACTGGCCTC CACTGCTAAG 
GAGGCGATGC AACAGCTGTG TGCCCGCCTG GAGCAGACAC ACGGGCGGCT CCTGGTGGCC 
CGCGTGCTGG GCTACATTGT GTCTTCCCGG CATGGGCTCT CAGAGGCGGA GCTAAAGGAT 
GTCCTGTCCT TGGATGACGA GGTCCTGCAG AGCGTGTACC AAGACTGGAC CCCACCCAGC 
AAACAGCTTC TGCGCTTCCC GCCCCTGCTG TGGGTGAGGC TTCGTCGAGA TCTGGGTAAC 
TGCTTGGCCA GGCGGCCGGT GGACGGCTTC ACCCTTCTGG CCATTGCCCA CAGACAGCTG 
GCCGAGGTGG TCCGAGAGCT CTACCTGTCT GGGCCTGAGA AAGCCAAGAG TCACGGAGTC 
TTGGCCGACT TCTTCTCAGG GGCCTGGAGC CAGGGCATCA AGAAACTCAT CACCCTGCCA 
CTCGTGGGAA AACCCCTGAA CCTGGACCGC AAGGTGGCCC CTCAGCCCCT GTGGTTCTCC 
GACACGGTTG CAAACCTGAG GAAGCTGAAG GAGTTACCCT ACCACTTAGT CCATGCGGGC 
CGTATAGAGG AGCTGAAGCA GGAGGTGCTG GGCTGCATGA CCTGGATTTG CTGCCGGGGC 
CTCTCTGGGG GCATCGAGGG TCTGCTGGAA GACTTTGACC TGTGTGCCCC GCACATGGAC 
TGTCCTGAGG TCAGCCTGGT CCGGGAAGCT CTCCACCTGT GCCGCCCGGC AGTGGAGCTC 
CGAGGCATGG AGAGAAGCGT CCTGTGCACA GAACTCCTGG CCAGACTCCA TTTCTTCACC 
ACTTCACATC CCGGGCTGGT GGGACAGCTG TGCCAACAGG CAGAGAGCTG GCTCCGGATG 
TGCCCACAGC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC CTCCGCCTGG AGGGCCCCTG 
CGGGCAACCC TCACCGGCTG TCACAAAGGC ATCACTGCCA TGGCATGGAG CCTGGAAGAG 
AAGCTGCTGC TGGTTGGCAC GCAGGAGGGC ATGGTGGCTG TGTGGGACAT GGAGGAACAG 
CAGGCAGTCC ACATCCTACC TGGACACACA GGAGAGGTGA GGTGTGTGGA AGTGTTTGCC 
CAAGGGACAC TTGCCGTCTC TGCCTCGAAA GACCACACCT TGCGCTTGTG GGACTTACTC 
TCTGGCCAGG AGAAATTCAC CATTTGGGAT GGAGGTTCAA AAGATCCCAC TGAATCTCAG 
ACCTGGAGCC TTCATGTGGA TGAAGCAAAG AAGGTTGTGT ATTCAGCTTC AGGCTCAAAG 
GTCAGTGCTT GGAATCTGGA AACTGCAGAG CAGATTTTCC ATATCCTGGG GGATGCCTCT 
GACCCCTGGG TATGCACGGC AGTGCTGGCT TCCCAGGCCA CCCTGCTGAC TGTGTCCCGG 
GGCGGCATGA TCAGTCTGTG GAGCTCAGCC ACGGGAACAC TGCACAGGCG GCAACGTTTG 
TCCAGCATCA AAGAAGATAC ACTTGCCTGC GGGGTCTCAG TCCAGAAACA AAGAATGATG 
GTTACTGGGT CCAGCACAGG CTCCATCTCT TTGGTTTCCT CCGAAGGAGA CAGCCTGCTG 
GAAAAGCTTC CGGAAGCTGT GGGGTTCCTG GTGGTATCTG AAGATGAGTC TCTTCTCGCT 
GCAGGTTTTG GAAGGTCTGT GAGGATATTT TTGGCAGATG CACAGGGATT TCATCGATTC 
ATGGCCACTG ACCTTGAACA TGAAGATGTG GTAGAGACAG CCACTTTTGG TCCTGAAAAC 
AATCTGATTG TCACTGGGTC CCGGGATGCA CTCATTCAGG TGTGGAGTCT GTCAGCCCAG 
GGGACTCTGC TGGACGTCCT GGACGGTGTC GGGGCCCCGG TGAGCCTGCT GGCCCGGGGC 
GGGGCCTTGG TGGCATCTGC TTCCCGTCAG TCCTCATCTT TTAAAGTCTG GGATCTCACC 
TGCGCTCAGA AGCCGAGGGC CTCTAACCCA TTCCTGGATC GCACTGGCCT CACGGCAGTC 
TCCCACAACG GCAGCTACGT CTACTTCCCT AAAACTGGGG ACAAAAACAA AGTCAGCATT 
TGGGCCTTGG CAGAAGGTGA GGAGCAAGAT GCTCTGGACA CCTCCAACGA GGTCAGATGT 
CTGGAGGTCG CTGAGCAGGC CAATCTCCTT TTCACTGGCC TCGTTTCAGG GGTTGTCCTG 
GTGTTCCCCC TGAATTCCAG ACAAGACGTG ATGTGTATCC CTCCTCCAGA AGCCCGGAAG 
GCCGTCAACT GCATGGCTCT CAGTAAGGAC GAGGGGCACC TGGCCATTGC CTATGACAGC 
ACCGTCTTGG TGCTGGACAT CAGCCCGGGG GACCCCTGTC CAGTCATCGA TGGGCCAAAA 
TACACCTTCT ATACCCAGCT GCCTGACACC ATAGCCAGTG TGGCTGCTCT GGCTGACTAT 
CGTGTCATCT ACGGCATGCT CAACGGCGAC CTCTTCCTTT ACGAGTGTGT GAATTCCAAG 
GTGTTCCCTC TGGAGGCCCA TGGGAGCCGA GTCACCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGCAG TCAGCGGGGC TGAGGAAGCC CTACTGTGTC TCTGGGACCT GCAGGCGTGC 
AAGTGGAAAT TCGAGATGAG CTACACGAAT TCTTACTGTG TAGGAGTCCA ATGTGCTTGC 
TTCTCCAAGG ATGACAAGTA TGTCTATGCA GGCTTGAAAG ATCGCTCCAT AATTGTCTGG 
AGTGTGCTGG ATGGCACACT GTTGGCTGTC CAGTTTGTCC ATGCCGTGGT GAACAGAATC 
ATCCCAACCT CCAATGGCTT CATTGCCCCC TCCAGACACG GCTATCTCAT CCGTGAGCGT 
TTCCAGTGCC CTTCGTCAAG ACTCTCACAA CAGGACCCTC TCAAGAACTT CAAGAAAGCA 
GTGTGGATGG TCAAATCCAG GCAGAGAGAA GAGCTGGCAG CTGCTGCAGG AGCACCCCAG 
GACTTGAGGT CAGAGAGTGC CCAGGGAAAT GAATCCAAAT CAAACAAACG CTCACAAGTC 
TGCCTGCTGG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_027969461.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027825262.1 Ovis aries NACHT and WD repeat domain containing 1 (NWD1), mRNA.

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CloneID	OSm80112
Clone ID Related Accession (Same CDS sequence)	XM_027969461.1
Accession Version	XM_027969461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	LOW QUALITY PROTEIN: NACHT domain- and WD repeat-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040256.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCAGAGAG GGAAGCCCTA CAGAGCACCG CCTACCCTGA AGTGCNAGAC CTTCTGCCAG 
AAGCACGGCC TGATGTTCGA GGTGGTTGAC CTGCGGTGGG GTATTCGGAA CACTGAGGCC 
ACTAACCCCC TGACTACAGA AATCTGCTTG GAGGAGCTGG ACCGGTGTCA GGAAATATCC 
ATTGGGCCAG CCTTTGTTGC CCTCGTGGGT GACCAGTACG GTCCCTGCCC AGTCCCCACA 
CGGATCGAGG AAAAGGAGTG GGAGGCGCTG AAGGCTCAGC TGACCACCAG GCCCCGCGAC 
CTGGAGCTGG TGGCACGAAG CTTCTGGAGG GACGAGAACA CGGTGCCCCC TGCCTACCTG 
CTGCAGGCCC TGGGCACTGG GGAGGCCTCT GGGCCTGAGG AGGCCACCCT GATCTCTGCA 
CTGCGCTCTG GAGCCCAGGA GGCCCACAGA CTGGGGCTTA TCAGCCAGGA GCAGTGGCAC 
CGCTACTACC GGTCAGTCAT CGAGTGGGAA ATAGAACGGG CCCTGCTGAG CTCAACGGTT 
GGGGATCACG GGGCAACAGT CTTCCTGAGA GATATCCAAG GCCTCAATAA ACACATCCTG 
GAAGACTGTG CCCTCAAAAT GGTGGACCGT CTTGCAGATG GTTGCCTGGA CACGGATGCC 
CAGAACCTTC TCAGCAACCT CAAGGGGCGC ATTGCTGACC GGCAGCCTGG AGTTCTCAAG 
GCCCACCACC TAGCGTGGAG CCGAGACCTG GTGAACCCCA AAAACAAGGC TCATGCCCGA 
TACCTGAAGG AGCTGGGGGA GCAGTTTGTG GCCAGGGCCA ACCATCAGGT TCTTGAGTGC 
CTCCGGGAGC TGGAGGCAGG CAGGCAGGAG TTGGCGTGGC TCTACCAGGA GATCCGCCAC 
CACCTGGGAC TCAGTGCCGA GGTCAACCGG ACCTTCTGTG GACGCCAGGA GCTCCTGACC 
CAGCTGGGGC AGCGACTGCG ACAGAGTGAT GGCCGCCCCC ACCCACCCCT GGTGCTTTTT 
GGACCCGCAG GCATCGGAAA GACGGCTCTG ATGTGCAAGC TGGCTGAGCA GATGTCGGGG 
CTGCTCGGCC GCAAGACAGT AACCATTCTG CGGCTTCTGG GGACATCTCA GATGAGCTCT 
GAGGCCCGAG GCCTGCTCCA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
CTGCCCCCTG CCCAGGTCCT AGAGGCCCAC ACCAGGCTGG TCCAGTTTTT CCACATCCTC 
CTCCACACCG TCTCCAGCCG AAACTTTGAG TCCCTTGTGA TCCTGCTGGA TTCGGTGGAT 
GAGGTGGACT CTGTCCACTG TGCTAGGAGG ATCCCCTGGC TGCCTCCCAA ATGTCCTCCA 
AGGGTCCACC TCATCGTCTC GGCCTGCTCT GGACAGCGGG GGGCTTTAGA CACCATGCGG 
CAGGTGCTCA CGGACCAAGG GGCTTACTGG GAGGTGAAAC CCCTCTCTGG AAACCAAGGG 
CAAGAGATGA TCCAGCTCCT GCTGGCCGCC TCGAGGAGGA CACTGAGTCA GGTGCAGAGG 
GACATGCTCT GGGCCAGCCT CCCAGAGTGT GGACACCCAG GGCGGCTGAG GCTGGCTTTC 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGCCC CACTGGCCTC CACTGCTAAG 
GAGGCGATGC AACAGCTGTG TGCCCGCCTG GAGCAGACAC ACGGGCGGCT CCTGGTGGCC 
CGCGTGCTGG GCTACATTGT GTCTTCCCGG CATGGGCTCT CAGAGGCGGA GCTAAAGGAT 
GTCCTGTCCT TGGATGATGA GGTCCTGCAG AGCGTGTACC AAGACTGGAC CCCACCCAGC 
AAACAGCTTC TGCGCTTCCC GCCCCTGCTG TGGGTGAGGC TTCGTCGAGA TCTGGGTAAC 
TGCTTGGCCA GGCGGCCGGT GGACGGCTTC ACCCTTCTGG CCATTGCCCA CAGACAGCTG 
GCCGAGGTGG TCCGAGAGCT CTACCTGTCT GGGCCTGAGA AAGCCAAGAG TCACGGAGTC 
TTGGCCGACT TCTTCTCAGG GGCCTGGAGC CAGGGCATCA AGAAACTCAT CACCCTGCCA 
CTCGTGGGAA AACCCCTGAA CCTGGACCGC AAGGTGGCCC CTCAGCCCCT GTGGTTCTCC 
GACACGGTTG CAAACCTGAG GAAGCTGAAG GAGTTACCCT ACCACTTAGT CCATGCGGGC 
CGTATAGAGG AGCTGAAGCA GGAGGTGCTG GGCAGCATGA CCTGGATTTG CTGCCGGGGC 
CTCTCTGGGG GCATCGAGGG TCTGCTGGAA GACTTTGACC TGTGTGCCCC GCACATGGAC 
TGTCCTGAGG TCAGCCTGGT CCGGGAAGCT CTCCAGCTGT GCCGCCCGGC AGTGGAGCTC 
CGAGGCATGG AGAGAAGCGT CCTGTGCACA GAACTCCTGG CCAGACTCCA TTTCTTCACC 
ACTTCACATC CCGGGCTGGT GGGACAGCTG TGCCAACAGG CACAGAGCTG GCTCCGGATG 
TGCCCACAGC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC CTCCGCCTGG AGGGCCCCTG 
CGGGCAACCC TCACCGGCTG TCACAAAGGC ATCACTGCCA TGGCATGGAG CCTGGAAGAG 
AAGCTGCTGC TGGTTGGCAC GCAGGAGGGC ATGGTGGCTG TGTGGGACAT GGAGGAACAG 
CAGGCAGTCC ACATCCTACC TGGACACACA GGAGAGGTGA GGTGTGTGGA AGTGTTTGCC 
CAAGGGACAC TTGCCATCTC TGCCTCGAAA GACCACACCT TGCGCTTGTG GGACTTACTC 
TCTGGCCAGG AGAAATTCAC CATTTGGGAT GGAGGTTCAA AAGATCCCAC TGAATCTCAG 
ACCTGGAGCC TTCATGTGGA TGAAGCAAAG AAGGTTGTGT ATTCAGCTTC AGGCTCAAAG 
GTCAGTGCTT GGAATCTGGA AACTGCAGAG CTGATTTTCC ATATCCTGGG GGATGCCTCT 
GACCCCTGGG TATGCACGGC AGTGCTGGCT TCCCAGGCCA CCCTGCTGAC TGTGTCCCGG 
GGCGGCATGA TCAGTCTGTG GAGCTCAGCC ACGGGAACAC TGCACAGGCG ACAACGTTTG 
TCCAGCATCA AAGAAGATAC ACTTGCCTGT GGGGTCTCAG TCCAGAAACA AAGAATGATG 
GTTACTGGGT CCAGCACAGG CTCCATCTCT TTGGTTTCCT CCGAAGGAGA CAGCCTGCTG 
GAAAAGCTTC CGGAAGCTGT GGGGTTCCTG GTGGTATCTG AAGATGAGTC TCTTCTCGCT 
GCAGGTTTTG GAAGGTCCGT GAGGATATTT TTGGCAGATG CACAGGGATT TCATCGATTC 
ATGGCCACTG ACCTTGAACA TGAAGATGTG GTAGAGACAG CCACTTTTGG TCCTGAAAAC 
AATCTGATTG TCACTGGGTC CCGGGATGCA CTCATTCAGG TGTGGAGTCT GTCAGCCCAG 
GGGACTCTGC TGGACGTCCT GGACGGTGTC GGGGCCCCGG TGAGCCTGCT GGCCCGGGGC 
GGGGCCTTGG TGGCATCTGC TTCCCGTCAG TCCTCATCTT TTAAAGTCTG GGATCTCACC 
TGCGCTCAGA AGCCGAGGGC CTCTAACCCA TTCCTGGATC GCACTGGCCT CACGGCAGTC 
TCCCACAACG GCAGCTACGT CTACTTCCCT AAAACTGGGG ACAAAAACAA AGTCAGCATT 
TGGGCCTTGG CAGAAGGTGA GGAGCAAGAT GCTCTGGACA CCTCCAACGA GGTCAGATGT 
CTGGAGGTCG CTGAGCAGGC CAATCTCCTT TTCACTGGCC TCGTTTCGGG GGTTGTCCTG 
GTGTTCCCCC TGAATTCCAG ACAAGACGTG ATGTGTATCC CTCCTCCAGA AGCCCGGAAG 
GCCGTCAACT GCATGGCTCT CAGTAAGGAC GAGGGGCACC TGGCCATTGC CTATGACAGC 
ACCATCTTGG TGCTGGACAT CAGCCCGGGG GACCCCTGTC CAGTCATCGA TGGGCCAAAA 
TACACCTTCT ATACCCAGCT GCCTGACACC ATAGCCAGTG TGGCTGCTCT GGCTGACTAT 
CGTGTCATCT ACGGCATGCT CAACGGCGAC CTCTTCCTTT ACGAGTGTGT GAATTCCAAG 
GTGTTCCCTC TGGAGGCCCA TGGGAGCCGA GTCACCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGCAG TCAGCGGGGC CGAGGAAGCC CTACTGTGTC TCTGGGACCT GCAGGCGTGC 
AAGTGGAAAT TCGAGATGAG CTACACGAAT TCTTACTGTG TAGGAGTCCA ATGTGCTTGC 
TTCTCCAAGG ATGACAAGTA TGTCTATGCA GGCTTGAAAG ATCGCTCCAT AATTGTCTGG 
AGTGTGCTGG ATGGCACACT GTTGGCTGTC CAGTTTGTCC ATGCCGTGGT GAACAGAATC 
ATCCCAACCT CCAATGGCTT CATTGCCCCC TCCAGACACG GCTATCTCAT CCGTGAGCGT 
TTCCAGTGCC CTTCGTCAAG ACTCTCACAA CAGGACCCTC TCAAGAACTT CAAGAAAGCA 
GTGTGGATGG TCAAATCCAG GCAGAGAGAA GAGCTGGCAG CTGCTGCAGG AGCACCCCAG 
GACTTGAGGT CAGAGAGTGC CCAGGGAAAT GAATCCAAAT CAAACAAACG CTCACAAGTC 
TGCCTGCTGG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027825262.1
CDS	93..4787
Translation	MQRGKPYRAPPTLKCnullTFCQKHGLMFEVVDLRWGIRNTEATNPLTTEICLEELDRCQEISIGPAFVALVGDQYGPCPVPTRIEEKEWEALKAQLTTRPRDLELVARSFWRDENTVPPAYLLQALGTGEASGPEEATLISALRSGAQEAHRLGLISQEQWHRYYRSVIEWEIERALLSSTVGDHGATVFLRDIQGLNKHILEDCALKMVDRLADGCLDTDAQNLLSNLKGRIADRQPGVLKAHHLAWSRDLVNPKNKAHARYLKELGEQFVARANHQVLECLRELEAGRQELAWLYQEIRHHLGLSAEVNRTFCGRQELLTQLGQRLRQSDGRPHPPLVLFGPAGIGKTALMCKLAEQMSGLLGRKTVTILRLLGTSQMSSEARGLLQSICFQVCLAYGLPLPPAQVLEAHTRLVQFFHILLHTVSSRNFESLVILLDSVDEVDSVHCARRIPWLPPKCPPRVHLIVSACSGQRGALDTMRQVLTDQGAYWEVKPLSGNQGQEMIQLLLAASRRTLSQVQRDMLWASLPECGHPGRLRLAFEEARKWASFTVPAPLASTAKEAMQQLCARLEQTHGRLLVARVLGYIVSSRHGLSEAELKDVLSLDDEVLQSVYQDWTPPSKQLLRFPPLLWVRLRRDLGNCLARRPVDGFTLLAIAHRQLAEVVRELYLSGPEKAKSHGVLADFFSGAWSQGIKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPYHLVHAGRIEELKQEVLGSMTWICCRGLSGGIEGLLEDFDLCAPHMDCPEVSLVREALQLCRPAVELRGMERSVLCTELLARLHFFTTSHPGLVGQLCQQAQSWLRMCPQPVLVPLGGFLPPPGGPLRATLTGCHKGITAMAWSLEEKLLLVGTQEGMVAVWDMEEQQAVHILPGHTGEVRCVEVFAQGTLAISASKDHTLRLWDLLSGQEKFTIWDGGSKDPTESQTWSLHVDEAKKVVYSASGSKVSAWNLETAELIFHILGDASDPWVCTAVLASQATLLTVSRGGMISLWSSATGTLHRRQRLSSIKEDTLACGVSVQKQRMMVTGSSTGSISLVSSEGDSLLEKLPEAVGFLVVSEDESLLAAGFGRSVRIFLADAQGFHRFMATDLEHEDVVETATFGPENNLIVTGSRDALIQVWSLSAQGTLLDVLDGVGAPVSLLARGGALVASASRQSSSFKVWDLTCAQKPRASNPFLDRTGLTAVSHNGSYVYFPKTGDKNKVSIWALAEGEEQDALDTSNEVRCLEVAEQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAVNCMALSKDEGHLAIAYDSTILVLDISPGDPCPVIDGPKYTFYTQLPDTIASVAALADYRVIYGMLNGDLFLYECVNSKVFPLEAHGSRVTCVEVSHKEQLAVSGAEEALLCLWDLQACKWKFEMSYTNSYCVGVQCACFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPSRHGYLIRERFQCPSSRLSQQDPLKNFKKAVWMVKSRQREELAAAAGAPQDLRSESAQGNESKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_027969461.1
Organism	Ovis aries(sheep)
Definition	Ovis aries NACHT and WD repeat domain containing 1 (NWD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027969461.1

ORF Insert Sequence:

ATGCAGAGAG GGAAGCCCTA CAGAGCACCG CCTACCCTGA AGTGCNAGAC CTTCTGCCAG 
AAGCACGGCC TGATGTTCGA GGTGGTTGAC CTGCGGTGGG GTATTCGGAA CACTGAGGCC 
ACTAACCCCC TGACTACAGA AATCTGCTTG GAGGAGCTGG ACCGGTGTCA GGAAATATCC 
ATTGGGCCAG CCTTTGTTGC CCTCGTGGGT GACCAGTACG GTCCCTGCCC AGTCCCCACA 
CGGATCGAGG AAAAGGAGTG GGAGGCGCTG AAGGCTCAGC TGACCACCAG GCCCCGCGAC 
CTGGAGCTGG TGGCACGAAG CTTCTGGAGG GACGAGAACA CGGTGCCCCC TGCCTACCTG 
CTGCAGGCCC TGGGCACTGG GGAGGCCTCT GGGCCTGAGG AGGCCACCCT GATCTCTGCA 
CTGCGCTCTG GAGCCCAGGA GGCCCACAGA CTGGGGCTTA TCAGCCAGGA GCAGTGGCAC 
CGCTACTACC GGTCAGTCAT CGAGTGGGAA ATAGAACGGG CCCTGCTGAG CTCAACGGTT 
GGGGATCACG GGGCAACAGT CTTCCTGAGA GATATCCAAG GCCTCAATAA ACACATCCTG 
GAAGACTGTG CCCTCAAAAT GGTGGACCGT CTTGCAGATG GTTGCCTGGA CACGGATGCC 
CAGAACCTTC TCAGCAACCT CAAGGGGCGC ATTGCTGACC GGCAGCCTGG AGTTCTCAAG 
GCCCACCACC TAGCGTGGAG CCGAGACCTG GTGAACCCCA AAAACAAGGC TCATGCCCGA 
TACCTGAAGG AGCTGGGGGA GCAGTTTGTG GCCAGGGCCA ACCATCAGGT TCTTGAGTGC 
CTCCGGGAGC TGGAGGCAGG CAGGCAGGAG TTGGCGTGGC TCTACCAGGA GATCCGCCAC 
CACCTGGGAC TCAGTGCCGA GGTCAACCGG ACCTTCTGTG GACGCCAGGA GCTCCTGACC 
CAGCTGGGGC AGCGACTGCG ACAGAGTGAT GGCCGCCCCC ACCCACCCCT GGTGCTTTTT 
GGACCCGCAG GCATCGGAAA GACGGCTCTG ATGTGCAAGC TGGCTGAGCA GATGTCGGGG 
CTGCTCGGCC GCAAGACAGT AACCATTCTG CGGCTTCTGG GGACATCTCA GATGAGCTCT 
GAGGCCCGAG GCCTGCTCCA GAGCATCTGC TTCCAGGTGT GCCTGGCCTA TGGGCTGCCC 
CTGCCCCCTG CCCAGGTCCT AGAGGCCCAC ACCAGGCTGG TCCAGTTTTT CCACATCCTC 
CTCCACACCG TCTCCAGCCG AAACTTTGAG TCCCTTGTGA TCCTGCTGGA TTCGGTGGAT 
GAGGTGGACT CTGTCCACTG TGCTAGGAGG ATCCCCTGGC TGCCTCCCAA ATGTCCTCCA 
AGGGTCCACC TCATCGTCTC GGCCTGCTCT GGACAGCGGG GGGCTTTAGA CACCATGCGG 
CAGGTGCTCA CGGACCAAGG GGCTTACTGG GAGGTGAAAC CCCTCTCTGG AAACCAAGGG 
CAAGAGATGA TCCAGCTCCT GCTGGCCGCC TCGAGGAGGA CACTGAGTCA GGTGCAGAGG 
GACATGCTCT GGGCCAGCCT CCCAGAGTGT GGACACCCAG GGCGGCTGAG GCTGGCTTTC 
GAGGAGGCCC GGAAGTGGGC CTCCTTCACT GTGCCAGCCC CACTGGCCTC CACTGCTAAG 
GAGGCGATGC AACAGCTGTG TGCCCGCCTG GAGCAGACAC ACGGGCGGCT CCTGGTGGCC 
CGCGTGCTGG GCTACATTGT GTCTTCCCGG CATGGGCTCT CAGAGGCGGA GCTAAAGGAT 
GTCCTGTCCT TGGATGATGA GGTCCTGCAG AGCGTGTACC AAGACTGGAC CCCACCCAGC 
AAACAGCTTC TGCGCTTCCC GCCCCTGCTG TGGGTGAGGC TTCGTCGAGA TCTGGGTAAC 
TGCTTGGCCA GGCGGCCGGT GGACGGCTTC ACCCTTCTGG CCATTGCCCA CAGACAGCTG 
GCCGAGGTGG TCCGAGAGCT CTACCTGTCT GGGCCTGAGA AAGCCAAGAG TCACGGAGTC 
TTGGCCGACT TCTTCTCAGG GGCCTGGAGC CAGGGCATCA AGAAACTCAT CACCCTGCCA 
CTCGTGGGAA AACCCCTGAA CCTGGACCGC AAGGTGGCCC CTCAGCCCCT GTGGTTCTCC 
GACACGGTTG CAAACCTGAG GAAGCTGAAG GAGTTACCCT ACCACTTAGT CCATGCGGGC 
CGTATAGAGG AGCTGAAGCA GGAGGTGCTG GGCAGCATGA CCTGGATTTG CTGCCGGGGC 
CTCTCTGGGG GCATCGAGGG TCTGCTGGAA GACTTTGACC TGTGTGCCCC GCACATGGAC 
TGTCCTGAGG TCAGCCTGGT CCGGGAAGCT CTCCAGCTGT GCCGCCCGGC AGTGGAGCTC 
CGAGGCATGG AGAGAAGCGT CCTGTGCACA GAACTCCTGG CCAGACTCCA TTTCTTCACC 
ACTTCACATC CCGGGCTGGT GGGACAGCTG TGCCAACAGG CACAGAGCTG GCTCCGGATG 
TGCCCACAGC CTGTGCTGGT GCCCCTCGGA GGATTCCTCC CTCCGCCTGG AGGGCCCCTG 
CGGGCAACCC TCACCGGCTG TCACAAAGGC ATCACTGCCA TGGCATGGAG CCTGGAAGAG 
AAGCTGCTGC TGGTTGGCAC GCAGGAGGGC ATGGTGGCTG TGTGGGACAT GGAGGAACAG 
CAGGCAGTCC ACATCCTACC TGGACACACA GGAGAGGTGA GGTGTGTGGA AGTGTTTGCC 
CAAGGGACAC TTGCCATCTC TGCCTCGAAA GACCACACCT TGCGCTTGTG GGACTTACTC 
TCTGGCCAGG AGAAATTCAC CATTTGGGAT GGAGGTTCAA AAGATCCCAC TGAATCTCAG 
ACCTGGAGCC TTCATGTGGA TGAAGCAAAG AAGGTTGTGT ATTCAGCTTC AGGCTCAAAG 
GTCAGTGCTT GGAATCTGGA AACTGCAGAG CTGATTTTCC ATATCCTGGG GGATGCCTCT 
GACCCCTGGG TATGCACGGC AGTGCTGGCT TCCCAGGCCA CCCTGCTGAC TGTGTCCCGG 
GGCGGCATGA TCAGTCTGTG GAGCTCAGCC ACGGGAACAC TGCACAGGCG ACAACGTTTG 
TCCAGCATCA AAGAAGATAC ACTTGCCTGT GGGGTCTCAG TCCAGAAACA AAGAATGATG 
GTTACTGGGT CCAGCACAGG CTCCATCTCT TTGGTTTCCT CCGAAGGAGA CAGCCTGCTG 
GAAAAGCTTC CGGAAGCTGT GGGGTTCCTG GTGGTATCTG AAGATGAGTC TCTTCTCGCT 
GCAGGTTTTG GAAGGTCCGT GAGGATATTT TTGGCAGATG CACAGGGATT TCATCGATTC 
ATGGCCACTG ACCTTGAACA TGAAGATGTG GTAGAGACAG CCACTTTTGG TCCTGAAAAC 
AATCTGATTG TCACTGGGTC CCGGGATGCA CTCATTCAGG TGTGGAGTCT GTCAGCCCAG 
GGGACTCTGC TGGACGTCCT GGACGGTGTC GGGGCCCCGG TGAGCCTGCT GGCCCGGGGC 
GGGGCCTTGG TGGCATCTGC TTCCCGTCAG TCCTCATCTT TTAAAGTCTG GGATCTCACC 
TGCGCTCAGA AGCCGAGGGC CTCTAACCCA TTCCTGGATC GCACTGGCCT CACGGCAGTC 
TCCCACAACG GCAGCTACGT CTACTTCCCT AAAACTGGGG ACAAAAACAA AGTCAGCATT 
TGGGCCTTGG CAGAAGGTGA GGAGCAAGAT GCTCTGGACA CCTCCAACGA GGTCAGATGT 
CTGGAGGTCG CTGAGCAGGC CAATCTCCTT TTCACTGGCC TCGTTTCGGG GGTTGTCCTG 
GTGTTCCCCC TGAATTCCAG ACAAGACGTG ATGTGTATCC CTCCTCCAGA AGCCCGGAAG 
GCCGTCAACT GCATGGCTCT CAGTAAGGAC GAGGGGCACC TGGCCATTGC CTATGACAGC 
ACCATCTTGG TGCTGGACAT CAGCCCGGGG GACCCCTGTC CAGTCATCGA TGGGCCAAAA 
TACACCTTCT ATACCCAGCT GCCTGACACC ATAGCCAGTG TGGCTGCTCT GGCTGACTAT 
CGTGTCATCT ACGGCATGCT CAACGGCGAC CTCTTCCTTT ACGAGTGTGT GAATTCCAAG 
GTGTTCCCTC TGGAGGCCCA TGGGAGCCGA GTCACCTGTG TGGAGGTCAG CCACAAGGAG 
CAGCTGGCAG TCAGCGGGGC CGAGGAAGCC CTACTGTGTC TCTGGGACCT GCAGGCGTGC 
AAGTGGAAAT TCGAGATGAG CTACACGAAT TCTTACTGTG TAGGAGTCCA ATGTGCTTGC 
TTCTCCAAGG ATGACAAGTA TGTCTATGCA GGCTTGAAAG ATCGCTCCAT AATTGTCTGG 
AGTGTGCTGG ATGGCACACT GTTGGCTGTC CAGTTTGTCC ATGCCGTGGT GAACAGAATC 
ATCCCAACCT CCAATGGCTT CATTGCCCCC TCCAGACACG GCTATCTCAT CCGTGAGCGT 
TTCCAGTGCC CTTCGTCAAG ACTCTCACAA CAGGACCCTC TCAAGAACTT CAAGAAAGCA 
GTGTGGATGG TCAAATCCAG GCAGAGAGAA GAGCTGGCAG CTGCTGCAGG AGCACCCCAG 
GACTTGAGGT CAGAGAGTGC CCAGGGAAAT GAATCCAAAT CAAACAAACG CTCACAAGTC 
TGCCTGCTGG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NWD1 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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