DHX8 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHX8
Entrez Gene ID	101110072
Full Name	DEAH-box helicase 8
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012172735.2	XP_012028125.1	ATP-dependent RNA helicase DHX8 isoform X2
XM_027974462.1	XP_027830263.1	ATP-dependent RNA helicase DHX8 isoform X1
XM_027974463.1	XP_027830264.1	ATP-dependent RNA helicase DHX8 isoform X2
XM_012157344.1	XP_012012734.1	ATP-dependent RNA helicase DHX8 isoform X3
XM_012186169.2	XP_012041559.1	ATP-dependent RNA helicase DHX8 isoform X1

Pathways from BioSystems
Gene Ontology

The following DHX8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv40480	XM_012172735.2 Latest version!	Ovis aries musimon DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OOv30679	XM_012157344.1 Latest version!	Ovis aries musimon DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSm60327	XM_027974462.1 Latest version!	Ovis aries DEAH-box helicase 8 (DHX8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSm10059	XM_012186169.2 Latest version!	Ovis aries DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm60328	XM_027974463.1 Latest version!	Ovis aries DEAH-box helicase 8 (DHX8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHX8 ( XM_012172735.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012028125.1 Ovis aries musimon DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv40480
Clone ID Related Accession (Same CDS sequence)	XM_012172735.2
Accession Version	XM_012172735.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ATP-dependent RNA helicase DHX8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011944418.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012172735.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG CTGTGGCCAT GGCAGGAGCC GTACCCGGGA CAGAGCTAGG CCCCGCGGAA 
GAACTCGCCA AACTCGAGTA TCTGTCTTTG GTGTCAAAGG TTTGCACCGA GCTGGACAAT 
CACTTAGGTA TCAACGACAA GGATTTGGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTCAA GGCTTCGCTG GTCAAAAATG GTGCAGAATT TACGGATTCA 
CTCATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCAAA GCCTTCTACT 
AGCAAAGATC CAGTTGTTAA GCCCAAGACT GAAAAAGAAA AGCTGAAGGA GCTCTTCCCA 
GTCCTTTGCC AGCCAGACAA CCCTTCTGTT CGGACCATGC TGGACGAGGA TGATATGAAA 
GTTGCTGTGG ATGTCCTAAA AGAACTGGAA GCCTTGATGC CCAGTGCAGC AGGCCAGGAG 
AAGCACAGAG ACACCGAGCA CCGGGACAAG GCCAAAAAAA AGAAGCGCAG CCGGAGCCGA 
GAGCGAGACC GTGACCGCGA GCGAGAACGT GATAGAGACC ATAAGCGGAG ACACCGGTCC 
CGCTCTCGAT CACGTTCCCG GACCCGGGAC AGGAATAAAG GGAAGTCTAG ATACCGGTCC 
AGGAGCAGAA GTCAAAGTCC CCCCAGAGAT CGGAAAGACC GAGACAAGTA TGGGGAGCGG 
AATCTGGACA GATGGCGGGA TAAACACGTG GACCGCCCTC CCCCAGAAGA ACCTGCCATT 
GGGGACATTT ACAACGGCAA GGTGACCAGC ATCATGCAGT TTGGATGTTT CGTGCAGCTG 
GAGGGACTGA GGAAGCGGTG GGAAGGCCTG GTGCACATCT CTGAGCTCCG GCGGGAAGGC 
CGTGTGGCCA ATGTGGCTGA CGTGGTGAGC AAAGGCCAGA GGGTCAAGGT CAAAGTGCTG 
TCCTTCACTG GGACCAAAAC CAGCTTGAGC ATGAAGGATG TGGATCAAGA GACTGGAGAA 
GATCTAAACC CAAATCGACG GCGGAATCTT GTCGGGGAGA CCAACGAAGA GACCTCTATG 
AGGAATCCGG ACAGGCCTAC CCACCTGTCC CTCGTCAGTG CCCCTGAAGT AGAGGATGAC 
TCACTAGAGC GCAAGCGCCT TACCCGCATC TCTGACCCAG AGAAGTGGGA GATCAAGCAG 
ATGATTGCTG CCAATGTCCT TTCCAAGGAA GAGTTCCCAG ACTTCGATGA AGAGACTGGC 
ATTCTCCCTA AAGTGGATGA TGAAGAAGGT AACCAGCCAC TTAGATGGAC CAGGATGATG 
GATTGGGAGG ACAGTTGGCA AGTTCAACCA TGGATAGGGT TTATTTGTAG TTTAAACCAG 
TGGTTAAACT CATCTGTTTC TTGGGTCTCA CAGCTCTTCT CTCAAAGAAC AAGGGTCCTT 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012028125.1
CDS	187..1632
Translation	MAVAVAMAGAVPGTELGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDMKVAVDVLKELEALMPSAAGQEKHRDTEHRDKAKKKKRSRSRERDRDRERERDRDHKRRHRSRSRSRSRTRDRNKGKSRYRSRSRSQSPPRDRKDRDKYGERNLDRWRDKHVDRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEGNQPLRWTRMMDWEDSWQVQPWIGFICSLNQWLNSSVSWVSQLFSQRTRVLS

Target ORF information:

RefSeq Version	XM_012172735.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012172735.2

ORF Insert Sequence:

ATGGCGGTGG CTGTGGCCAT GGCAGGAGCC GTACCCGGGA CAGAGCTAGG CCCCGCGGAA 
GAACTCGCCA AACTCGAGTA TCTGTCTTTG GTGTCAAAGG TTTGCACCGA GCTGGACAAT 
CACTTAGGTA TCAACGACAA GGATTTGGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTCAA GGCTTCGCTG GTCAAAAATG GTGCAGAATT TACGGATTCA 
CTCATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCAAA GCCTTCTACT 
AGCAAAGATC CAGTTGTTAA GCCCAAGACT GAAAAAGAAA AGCTGAAGGA GCTCTTCCCA 
GTCCTTTGCC AGCCAGACAA CCCTTCTGTT CGGACCATGC TGGACGAGGA TGATATGAAA 
GTTGCTGTGG ATGTCCTAAA AGAACTGGAA GCCTTGATGC CCAGTGCAGC AGGCCAGGAG 
AAGCACAGAG ACACCGAGCA CCGGGACAAG GCCAAAAAAA AGAAGCGCAG CCGGAGCCGA 
GAGCGAGACC GTGACCGCGA GCGAGAACGT GATAGAGACC ATAAGCGGAG ACACCGGTCC 
CGCTCTCGAT CACGTTCCCG GACCCGGGAC AGGAATAAAG GGAAGTCTAG ATACCGGTCC 
AGGAGCAGAA GTCAAAGTCC CCCCAGAGAT CGGAAAGACC GAGACAAGTA TGGGGAGCGG 
AATCTGGACA GATGGCGGGA TAAACACGTG GACCGCCCTC CCCCAGAAGA ACCTGCCATT 
GGGGACATTT ACAACGGCAA GGTGACCAGC ATCATGCAGT TTGGATGTTT CGTGCAGCTG 
GAGGGACTGA GGAAGCGGTG GGAAGGCCTG GTGCACATCT CTGAGCTCCG GCGGGAAGGC 
CGTGTGGCCA ATGTGGCTGA CGTGGTGAGC AAAGGCCAGA GGGTCAAGGT CAAAGTGCTG 
TCCTTCACTG GGACCAAAAC CAGCTTGAGC ATGAAGGATG TGGATCAAGA GACTGGAGAA 
GATCTAAACC CAAATCGACG GCGGAATCTT GTCGGGGAGA CCAACGAAGA GACCTCTATG 
AGGAATCCGG ACAGGCCTAC CCACCTGTCC CTCGTCAGTG CCCCTGAAGT AGAGGATGAC 
TCACTAGAGC GCAAGCGCCT TACCCGCATC TCTGACCCAG AGAAGTGGGA GATCAAGCAG 
ATGATTGCTG CCAATGTCCT TTCCAAGGAA GAGTTCCCAG ACTTCGATGA AGAGACTGGC 
ATTCTCCCTA AAGTGGATGA TGAAGAAGGT AACCAGCCAC TTAGATGGAC CAGGATGATG 
GATTGGGAGG ACAGTTGGCA AGTTCAACCA TGGATAGGGT TTATTTGTAG TTTAAACCAG 
TGGTTAAACT CATCTGTTTC TTGGGTCTCA CAGCTCTTCT CTCAAAGAAC AAGGGTCCTT 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( XM_012157344.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012012734.1 Ovis aries musimon DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOv30679
Clone ID Related Accession (Same CDS sequence)	XM_012157344.1
Accession Version	XM_012157344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	ATP-dependent RNA helicase DHX8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942831.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTATG TGAGAATGAT TGGTCATTTA TGTCTTTTGT TTCTGGCTAA TTCTTCTTTT 
GGGGGATTTC CAGATGAGGA CCTTGAGATT GAGCTGGTTG AGGAAGAGCC TCCATTCCTG 
AGAGGGCACA CCAAGCAGAG CATGGACATG AGCCCCATCA AAATCGTTAA GAATCCAGAT 
GGCTCCCTCT CCCAAGCAGC AATGATGCAG AGTGCCTTGG CCAAAGAAAG GCGGGAACTT 
AAGCAAGCAC AGCGGGAAGC TGAGATGGAT TCTATTCCCA TGGGACTCAA CAAACATTGG 
GTTGATCCGC TGCCTGATGC GGAAGGCAGG CAGATTGCTG CCAACATGAG GGGTATTGGG 
ATGATGCCCA ATGACATTCC TGAGTGGAAG AAGCATGCCT TTGGGGGCAA CAAAGCTTCA 
TATGGAAAAA AGACCCAGAT GTCAATCATT GAACAAAGGG AGAGCCTCCC CATCTACAAG 
CTGAAGGAGC AACTGGTCCA GGCTGTCCAT GACAACCAGA TCCTGATTGT TATTGGAGAG 
ACCGGGTCTG GGAAGACCAC GCAGATCACC CAGTACCTGG CGGAGGCAGG CTACACTTCC 
AGGGGCAAGA TTGGATGTAC CCAGCCTAGA AGAGTAGCGG CCATGTCTGT GGCCAAAAGA 
GTGTCAGAGG AGTTTGGTTG TTGCTTAGGC CAAGAGGTGG GCTACACCAT TCGATTTGAG 
GACTGCACCA GCCCTGAAAC AGTCATCAAG TACATGACAG ACGGGATGTT GCTCCGAGAA 
TGCCTGATCG ACCCCGACCT CACTCAGTAT GCAATCATCA TGTTGGATGA GGCGCACGAG 
AGGACAATTC ATACTGATGT GCTCTTTGGA TTGCTGAAAA AGACGGTTCA GAAACGGCAG 
GACATGAAAC TGATTGTCAC CTCAGCCACC TTGGATGCTG TGAAGTTTTC TCAGTACTTC 
TATGAAGCTC CCATCTTTAC CATCCCAGGT CGAACATACC CAGTGGAAAT ATTGTACACA 
AAGGAACCTG AAACCGATTA TCTAGATGCC AGCTTGATCA CAGTCATGCA GATCCACTTA 
ACAGAACCAC CAGGTGATAT CCTGGTCTTC CTGACTGGTC AGGAAGAGAT CGATACTGCT 
TGCGAGATTC TGTATGAAAG AATGAAATCC CTGGGACCTG ATGTTCCAGA GCTGATTATC 
CTCCCAGTGT ACTCTGCTCT CCCCAGTGAG ATGCAGACCA GAATCTTTGA CCCAGCTCCA 
CCTGGCAGCA GAAAGGTTGT GATTGCCACC AACATTGCAG AGACATCGCT GACTATTGAT 
GGCATTTACT ATGTGGTAGA CCCTGGATTC GTGAAGCAGA AGGTGTACAA TTCTAAGACA 
GGGATTGACC AGCTCGTGGT GACTCCTATT TCTCAGGCTC AGGCAAAGCA ACGAGCTGGC 
AGAGCTGGGA GAACAGGCCC AGGGAAGTGT TATAGGCTGT ACACAGAACG TGCCTACCGA 
GATGAAATGC TGACCACCAA CGTGCCAGAA ATTCAGAGAA CCAACTTAGC AAGCACAGTG 
CTCTCGCTCA AGGCAATGGG CATCAATGAC CTGCTGTCGT TTGACTTCAT GGATGCCCCG 
CCAATGGAAA CCTTGATCAC AGCCATGGAG CAGCTGTACA CGCTGGGGGC CCTGGACGAC 
GAGGGCCTGC TTACTCGCCT GGGCCGCAGG ATGGCAGAGT TCCCTCTGGA GCCAATGCTG 
TGCAAAATGC TGATCATGTC TGTGCATCTA GGCTGCAGTG AGGAAATGCT GACCATTGTG 
TCCATGCTGT CTGTGCAGAA CGTCTTCTAC AGGCCCAAAG ATAAACAAGC CCTTGCAGAT 
CAGAAGAAGG CCAAATTCCA CCAGACCGAA GGGGACCACC TCACCCTGCT GGCCGTGTAC 
AACTCTTGGA AGAACAACAA GTTCTCCAAC CCATGGTGCT ATGAGAACTT TATCCAGGCT 
CGTTCTCTGC GCCGGGCCCA GGACATTCGC AAGCAGATGT TGGGCATAAT GGACAGACAC 
AAGCTGGATG TGGTGTCCTG TGGTAAGTCC ACAGTCCGAG TGCAGAAGGC CATCTGCAGT 
GGATTCTTCC GGAACGCTGC CAAGAAAGAC CCACAGGAGG GTTACCGGAC ACTGATTGAC 
CAGCAAGTGG TCTACATCCA TCCGTCCAGT GCTCTCTTCA ACAGACAGCC CGAATGGGTG 
GTGTATCATG AGCTGGTGCT GACTACGAAG GAGTACATGC GTGAGGTCAC CACCATCGAT 
CCCCGGTGGC TTGTGGAGTT TGCACCGGCC TTCTTCAAGG TCTCTGACCC GACCAAGCTA 
AGCAAGCAGA AGAAGCAGCA ACGGCTGGAA CCCTTGTACA ACCGCTATGA GGAGCCCAAT 
GCCTGGAGAA TATCCCGAGC CTTCCGGCGA CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012012734.1
CDS	958..3393
Translation	MVYVRMIGHLCLLFLANSSFGGFPDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIIEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_012157344.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012157344.1

ORF Insert Sequence:

ATGGTTTATG TGAGAATGAT TGGTCATTTA TGTCTTTTGT TTCTGGCTAA TTCTTCTTTT 
GGGGGATTTC CAGATGAGGA CCTTGAGATT GAGCTGGTTG AGGAAGAGCC TCCATTCCTG 
AGAGGGCACA CCAAGCAGAG CATGGACATG AGCCCCATCA AAATCGTTAA GAATCCAGAT 
GGCTCCCTCT CCCAAGCAGC AATGATGCAG AGTGCCTTGG CCAAAGAAAG GCGGGAACTT 
AAGCAAGCAC AGCGGGAAGC TGAGATGGAT TCTATTCCCA TGGGACTCAA CAAACATTGG 
GTTGATCCGC TGCCTGATGC GGAAGGCAGG CAGATTGCTG CCAACATGAG GGGTATTGGG 
ATGATGCCCA ATGACATTCC TGAGTGGAAG AAGCATGCCT TTGGGGGCAA CAAAGCTTCA 
TATGGAAAAA AGACCCAGAT GTCAATCATT GAACAAAGGG AGAGCCTCCC CATCTACAAG 
CTGAAGGAGC AACTGGTCCA GGCTGTCCAT GACAACCAGA TCCTGATTGT TATTGGAGAG 
ACCGGGTCTG GGAAGACCAC GCAGATCACC CAGTACCTGG CGGAGGCAGG CTACACTTCC 
AGGGGCAAGA TTGGATGTAC CCAGCCTAGA AGAGTAGCGG CCATGTCTGT GGCCAAAAGA 
GTGTCAGAGG AGTTTGGTTG TTGCTTAGGC CAAGAGGTGG GCTACACCAT TCGATTTGAG 
GACTGCACCA GCCCTGAAAC AGTCATCAAG TACATGACAG ACGGGATGTT GCTCCGAGAA 
TGCCTGATCG ACCCCGACCT CACTCAGTAT GCAATCATCA TGTTGGATGA GGCGCACGAG 
AGGACAATTC ATACTGATGT GCTCTTTGGA TTGCTGAAAA AGACGGTTCA GAAACGGCAG 
GACATGAAAC TGATTGTCAC CTCAGCCACC TTGGATGCTG TGAAGTTTTC TCAGTACTTC 
TATGAAGCTC CCATCTTTAC CATCCCAGGT CGAACATACC CAGTGGAAAT ATTGTACACA 
AAGGAACCTG AAACCGATTA TCTAGATGCC AGCTTGATCA CAGTCATGCA GATCCACTTA 
ACAGAACCAC CAGGTGATAT CCTGGTCTTC CTGACTGGTC AGGAAGAGAT CGATACTGCT 
TGCGAGATTC TGTATGAAAG AATGAAATCC CTGGGACCTG ATGTTCCAGA GCTGATTATC 
CTCCCAGTGT ACTCTGCTCT CCCCAGTGAG ATGCAGACCA GAATCTTTGA CCCAGCTCCA 
CCTGGCAGCA GAAAGGTTGT GATTGCCACC AACATTGCAG AGACATCGCT GACTATTGAT 
GGCATTTACT ATGTGGTAGA CCCTGGATTC GTGAAGCAGA AGGTGTACAA TTCTAAGACA 
GGGATTGACC AGCTCGTGGT GACTCCTATT TCTCAGGCTC AGGCAAAGCA ACGAGCTGGC 
AGAGCTGGGA GAACAGGCCC AGGGAAGTGT TATAGGCTGT ACACAGAACG TGCCTACCGA 
GATGAAATGC TGACCACCAA CGTGCCAGAA ATTCAGAGAA CCAACTTAGC AAGCACAGTG 
CTCTCGCTCA AGGCAATGGG CATCAATGAC CTGCTGTCGT TTGACTTCAT GGATGCCCCG 
CCAATGGAAA CCTTGATCAC AGCCATGGAG CAGCTGTACA CGCTGGGGGC CCTGGACGAC 
GAGGGCCTGC TTACTCGCCT GGGCCGCAGG ATGGCAGAGT TCCCTCTGGA GCCAATGCTG 
TGCAAAATGC TGATCATGTC TGTGCATCTA GGCTGCAGTG AGGAAATGCT GACCATTGTG 
TCCATGCTGT CTGTGCAGAA CGTCTTCTAC AGGCCCAAAG ATAAACAAGC CCTTGCAGAT 
CAGAAGAAGG CCAAATTCCA CCAGACCGAA GGGGACCACC TCACCCTGCT GGCCGTGTAC 
AACTCTTGGA AGAACAACAA GTTCTCCAAC CCATGGTGCT ATGAGAACTT TATCCAGGCT 
CGTTCTCTGC GCCGGGCCCA GGACATTCGC AAGCAGATGT TGGGCATAAT GGACAGACAC 
AAGCTGGATG TGGTGTCCTG TGGTAAGTCC ACAGTCCGAG TGCAGAAGGC CATCTGCAGT 
GGATTCTTCC GGAACGCTGC CAAGAAAGAC CCACAGGAGG GTTACCGGAC ACTGATTGAC 
CAGCAAGTGG TCTACATCCA TCCGTCCAGT GCTCTCTTCA ACAGACAGCC CGAATGGGTG 
GTGTATCATG AGCTGGTGCT GACTACGAAG GAGTACATGC GTGAGGTCAC CACCATCGAT 
CCCCGGTGGC TTGTGGAGTT TGCACCGGCC TTCTTCAAGG TCTCTGACCC GACCAAGCTA 
AGCAAGCAGA AGAAGCAGCA ACGGCTGGAA CCCTTGTACA ACCGCTATGA GGAGCCCAAT 
GCCTGGAGAA TATCCCGAGC CTTCCGGCGA CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( XM_027974462.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027830263.1 Ovis aries DEAH-box helicase 8 (DHX8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm60327
Clone ID Related Accession (Same CDS sequence)	XM_027974462.1
Accession Version	XM_027974462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	ATP-dependent RNA helicase DHX8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTG GATGTTTCGT GCAGCTGGAG GGACTGAGGA AGCGGTGGGA AGGCCTGGTG 
CACATCTCTG AGCTCCGGCG GGAAGGCCGT GTGGCCAATG TGGCTGATGT GGTGAGCAAA 
GGCCAGAGGG TCAAGGTCAA AGTGCTGTCC TTCACTGGGA CCAAAACCAG CTTGAGCATG 
AAGGATGTGG ATCAAGAGAC TGGAGAAGAT CTAAACCCAA ATCGACGGCG GAATCTTGTC 
GGGGAGACCA ACGAAGAGAC CTCTATGAGG AATCCGGACA GGCCTACCCA CCTGTCCCTC 
GTCAGTGCCC CTGAAGTAGA GGATGACTCA CTAGAGCGCA AGCGCCTTAC CCGCATCTCT 
GACCCAGAGA AGTGGGAGAT CAAGCAGATG ATTGCTGCCA ATGTCCTTTC CAAGGAAGAG 
TTCCCAGACT TCGATGAAGA GACTGGCATT CTCCCTAAAG TGGATGATGA AGAAGATGAG 
GACCTTGAGA TTGAGCTGGT TGAGGAAGAG CCTCCATTCC TGAGAGGGCA CACCAAGCAG 
AGCATGGACA TGAGCCCTAT CAAAATCGTT AAGAATCCAG ATGGCTCCCT CTCCCAAGCA 
GCAATGATGC AGAGTGCCTT GGCCAAAGAA AGGCGGGAAC TTAAGCAAGC ACAGCGGGAA 
GCTGAGATGG ATTCTATTCC CATGGGACTC AACAAACATT GGGTTGATCC GCTGCCTGAT 
GCGGAAGGCA GGCAGATTGC TGCCAACATG AGGGGTATTG GGATGATGCC CAATGACATT 
CCTGAATGGA AGAAGCATGC CTTTGGGGGC AACAAAGCTT CATATGGAAA AAAGACCCAG 
ATGTCAATCA TTGAACAAAG GGAGAGCCTC CCCATCTACA AGCTGAAGGA GCAACTGGTC 
CAGGCTGTCC ATGACAACCA GATCCTGATT GTTATTGGAG AGACCGGGTC TGGGAAGACC 
ACGCAGATCA CCCAGTACCT GGCAGAGGCA GGCTACACTT CCAGGGGCAA GATTGGATGT 
ACCCAGCCTA GAAGAGTAGC GGCCATGTCT GTGGCCAAAA GAGTGTCAGA GGAGTTCGGT 
TGTTGCTTAG GCCAAGAGGT GGGCTACACC ATTCGATTTG AGGACTGCAC CAGCCCTGAA 
ACAGTCATCA AGTACATGAC AGACGGGATG TTGCTCCGAG AATGCCTGAT CGACCCCGAC 
CTCACTCAGT ATGCAATCAT CATGTTGGAC GAGGCGCATG AGAGGACAAT TCATACTGAT 
GTGCTCTTTG GATTGCTGAA AAAGACGGTT CAGAAACGGC AGGACATGAA ACTGATTGTC 
ACCTCAGCCA CCTTGGATGC TGTGAAGTTT TCTCAGTACT TCTATGAAGC TCCCATCTTT 
ACCATCCCAG GTCGAACATA CCCAGTGGAA ATATTGTACA CAAAGGAACC TGAAACCGAT 
TATCTAGATG CCAGCTTGAT CACAGTCATG CAGATCCACT TAACAGAACC GCCAGGTGAT 
ATCCTGGTCT TCCTGACTGG TCAGGAAGAG ATCGATACTG CTTGCGAGAT TCTGTATGAA 
AGAATGAAAT CCCTGGGACC TGATGTTCCA GAGCTGATTA TCCTCCCAGT GTACTCTGCT 
CTCCCCAGTG AGATGCAGAC CAGAATCTTT GACCCAGCTC CACCTGGCAG CAGAAAGGTT 
GTGATTGCCA CCAACATTGC AGAGACATCG CTGACTATTG ATGGCATTTA CTATGTGGTA 
GACCCTGGAT TCGTGAAGCA GAAGGTGTAC AATTCTAAGA CAGGGATTGA CCAGCTCGTG 
GTGACTCCTA TTTCTCAGGC TCAGGCAAAG CAACGAGCTG GCAGAGCTGG GAGAACAGGC 
CCAGGGAAGT GTTATAGGCT GTACACAGAA CGTGCCTACC GAGATGAAAT GCTGACCACC 
AATGTGCCAG AAATTCAGAG AACCAACTTA GCAAGCACAG TGCTCTCGCT CAAGGCCATG 
GGCATCAATG ACCTGCTGTC GTTTGACTTC ATGGATGCCC CGCCAATGGA AACCTTGATC 
ACAGCCATGG AGCAGCTGTA CACGCTGGGG GCCCTGGACG ACGAGGGCCT GCTTACTCGC 
CTGGGCCGCA GGATGGCAGA GTTCCCTCTG GAGCCAATGC TGTGCAAAAT GCTGATCATG 
TCTGTGCATC TAGGCTGCAG TGAGGAAATG CTGACCATTG TGTCCATGCT GTCTGTGCAG 
AACGTCTTCT ACAGGCCCAA AGATAAACAA GCCCTTGCAG ATCAGAAGAA GGCCAAATTC 
CACCAGACCG AAGGGGACCA CCTCACCCTG CTGGCCGTTT ACAACTCTTG GAAGAACAAC 
AAGTTCTCCA ACCCATGGTG CTATGAGAAC TTTATCCAGG CTCGTTCTCT GCGCCGGGCC 
CAGGACATTC GCAAGCAGAT GTTGGGCATA ATGGACAGAC ACAAGCTGGA TGTGGTGTCC 
TGTGGTAAGT CCACAGTCCG AGTGCAGAAG GCCATCTGCA GTGGATTCTT CCGGAATGCT 
GCCAAGAAAG ACCCACAGGA GGGTTACCGG ACACTGATTG ACCAGCAAGT GGTCTACATC 
CATCCGTCCA GTGCTCTCTT CAACAGACAG CCCGAATGGG TGGTATATCA TGAGCTGGTG 
CTGACTACGA AGGAGTACAT GCGTGAGGTC ACCACCATCG ACCCCCGGTG GCTTGTGGAG 
TTTGCACCGG CCTTCTTCAA GGTCTCTGAC CCGACCAAGC TAAGCAAGCA GAAGAAGCAG 
CAACGGCTGG AACCCTTGTA CAACCGCTAT GAGGAGCCCA ATGCCTGGAG AATATCCCGA 
GCCTTCCGGC GACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027830263.1
CDS	398..3235
Translation	MQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIIEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_027974462.1
Organism	Ovis aries(sheep)
Definition	Ovis aries DEAH-box helicase 8 (DHX8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027974462.1

ORF Insert Sequence:

ATGCAGTTTG GATGTTTCGT GCAGCTGGAG GGACTGAGGA AGCGGTGGGA AGGCCTGGTG 
CACATCTCTG AGCTCCGGCG GGAAGGCCGT GTGGCCAATG TGGCTGATGT GGTGAGCAAA 
GGCCAGAGGG TCAAGGTCAA AGTGCTGTCC TTCACTGGGA CCAAAACCAG CTTGAGCATG 
AAGGATGTGG ATCAAGAGAC TGGAGAAGAT CTAAACCCAA ATCGACGGCG GAATCTTGTC 
GGGGAGACCA ACGAAGAGAC CTCTATGAGG AATCCGGACA GGCCTACCCA CCTGTCCCTC 
GTCAGTGCCC CTGAAGTAGA GGATGACTCA CTAGAGCGCA AGCGCCTTAC CCGCATCTCT 
GACCCAGAGA AGTGGGAGAT CAAGCAGATG ATTGCTGCCA ATGTCCTTTC CAAGGAAGAG 
TTCCCAGACT TCGATGAAGA GACTGGCATT CTCCCTAAAG TGGATGATGA AGAAGATGAG 
GACCTTGAGA TTGAGCTGGT TGAGGAAGAG CCTCCATTCC TGAGAGGGCA CACCAAGCAG 
AGCATGGACA TGAGCCCTAT CAAAATCGTT AAGAATCCAG ATGGCTCCCT CTCCCAAGCA 
GCAATGATGC AGAGTGCCTT GGCCAAAGAA AGGCGGGAAC TTAAGCAAGC ACAGCGGGAA 
GCTGAGATGG ATTCTATTCC CATGGGACTC AACAAACATT GGGTTGATCC GCTGCCTGAT 
GCGGAAGGCA GGCAGATTGC TGCCAACATG AGGGGTATTG GGATGATGCC CAATGACATT 
CCTGAATGGA AGAAGCATGC CTTTGGGGGC AACAAAGCTT CATATGGAAA AAAGACCCAG 
ATGTCAATCA TTGAACAAAG GGAGAGCCTC CCCATCTACA AGCTGAAGGA GCAACTGGTC 
CAGGCTGTCC ATGACAACCA GATCCTGATT GTTATTGGAG AGACCGGGTC TGGGAAGACC 
ACGCAGATCA CCCAGTACCT GGCAGAGGCA GGCTACACTT CCAGGGGCAA GATTGGATGT 
ACCCAGCCTA GAAGAGTAGC GGCCATGTCT GTGGCCAAAA GAGTGTCAGA GGAGTTCGGT 
TGTTGCTTAG GCCAAGAGGT GGGCTACACC ATTCGATTTG AGGACTGCAC CAGCCCTGAA 
ACAGTCATCA AGTACATGAC AGACGGGATG TTGCTCCGAG AATGCCTGAT CGACCCCGAC 
CTCACTCAGT ATGCAATCAT CATGTTGGAC GAGGCGCATG AGAGGACAAT TCATACTGAT 
GTGCTCTTTG GATTGCTGAA AAAGACGGTT CAGAAACGGC AGGACATGAA ACTGATTGTC 
ACCTCAGCCA CCTTGGATGC TGTGAAGTTT TCTCAGTACT TCTATGAAGC TCCCATCTTT 
ACCATCCCAG GTCGAACATA CCCAGTGGAA ATATTGTACA CAAAGGAACC TGAAACCGAT 
TATCTAGATG CCAGCTTGAT CACAGTCATG CAGATCCACT TAACAGAACC GCCAGGTGAT 
ATCCTGGTCT TCCTGACTGG TCAGGAAGAG ATCGATACTG CTTGCGAGAT TCTGTATGAA 
AGAATGAAAT CCCTGGGACC TGATGTTCCA GAGCTGATTA TCCTCCCAGT GTACTCTGCT 
CTCCCCAGTG AGATGCAGAC CAGAATCTTT GACCCAGCTC CACCTGGCAG CAGAAAGGTT 
GTGATTGCCA CCAACATTGC AGAGACATCG CTGACTATTG ATGGCATTTA CTATGTGGTA 
GACCCTGGAT TCGTGAAGCA GAAGGTGTAC AATTCTAAGA CAGGGATTGA CCAGCTCGTG 
GTGACTCCTA TTTCTCAGGC TCAGGCAAAG CAACGAGCTG GCAGAGCTGG GAGAACAGGC 
CCAGGGAAGT GTTATAGGCT GTACACAGAA CGTGCCTACC GAGATGAAAT GCTGACCACC 
AATGTGCCAG AAATTCAGAG AACCAACTTA GCAAGCACAG TGCTCTCGCT CAAGGCCATG 
GGCATCAATG ACCTGCTGTC GTTTGACTTC ATGGATGCCC CGCCAATGGA AACCTTGATC 
ACAGCCATGG AGCAGCTGTA CACGCTGGGG GCCCTGGACG ACGAGGGCCT GCTTACTCGC 
CTGGGCCGCA GGATGGCAGA GTTCCCTCTG GAGCCAATGC TGTGCAAAAT GCTGATCATG 
TCTGTGCATC TAGGCTGCAG TGAGGAAATG CTGACCATTG TGTCCATGCT GTCTGTGCAG 
AACGTCTTCT ACAGGCCCAA AGATAAACAA GCCCTTGCAG ATCAGAAGAA GGCCAAATTC 
CACCAGACCG AAGGGGACCA CCTCACCCTG CTGGCCGTTT ACAACTCTTG GAAGAACAAC 
AAGTTCTCCA ACCCATGGTG CTATGAGAAC TTTATCCAGG CTCGTTCTCT GCGCCGGGCC 
CAGGACATTC GCAAGCAGAT GTTGGGCATA ATGGACAGAC ACAAGCTGGA TGTGGTGTCC 
TGTGGTAAGT CCACAGTCCG AGTGCAGAAG GCCATCTGCA GTGGATTCTT CCGGAATGCT 
GCCAAGAAAG ACCCACAGGA GGGTTACCGG ACACTGATTG ACCAGCAAGT GGTCTACATC 
CATCCGTCCA GTGCTCTCTT CAACAGACAG CCCGAATGGG TGGTATATCA TGAGCTGGTG 
CTGACTACGA AGGAGTACAT GCGTGAGGTC ACCACCATCG ACCCCCGGTG GCTTGTGGAG 
TTTGCACCGG CCTTCTTCAA GGTCTCTGAC CCGACCAAGC TAAGCAAGCA GAAGAAGCAG 
CAACGGCTGG AACCCTTGTA CAACCGCTAT GAGGAGCCCA ATGCCTGGAG AATATCCCGA 
GCCTTCCGGC GACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( XM_012186169.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012041559.1 Ovis aries DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), transcript variant X1, mRNA.

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CloneID	OSm10059
Clone ID Related Accession (Same CDS sequence)	XM_012186169.2
Accession Version	XM_012186169.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	ATP-dependent RNA helicase DHX8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012186169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG CTGTGGCCAT GGCAGGAGCC GTACCCGGGA CAGAGCTAGG CCCCGCGGAA 
GAACTCGCCA AACTCGAGTA TCTGTCTTTG GTGTCAAAGG TTTGCACCGA GCTGGACAAT 
CACTTAGGTA TCAACGACAA GGATTTGGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTCAA GGCTTCGCTG GTCAAAAATG GTGCAGAATT TACGGATTCA 
CTCATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCAAA GCCTTCTACT 
AGCAAAGATC CAGTTGTTAA GCCCAAGACT GAAAAAGAAA AGCTGAAGGA GCTCTTCCCA 
GTCCTTTGCC AGCCAGACAA CCCTTCTGTT CGGACCATGC TGGACGAGGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTAAA AGAACTGGAA GCCTTGATGC CCAGTGCAGC AGGCCAGGAG 
AAGCACAGAG ACACCGAGCA CCGGGACAAG GCCAAGAAAA AGAAGCGCAG CCGGAGCCGA 
GAGCGAGACC GTGACCGCGA GCGAGAACGT GATAGAGACC ATAAGCGGAG ACACCGGTCC 
CGCTCTCGAT CACGTTCCCG GACCCGGGAC AGGAATAAAG GGAAGTCTAG ATACCGGTCC 
AGGAGCAGAA GTCAAAGTCC CCCCAGAGAT CGGAAAGACC GAGACAAGTA TGGGGAGCGG 
AATCTGGACA GATGGCGGGA TAAACACGTG GACCGCCCTC CCCCAGAAGA ACCCGCCATT 
GGGGACATTT ACAACGGCAA GGTGACCAGC ATCATGCAGT TTGGATGTTT CGTGCAGCTG 
GAGGGACTGA GGAAGCGGTG GGAAGGCCTG GTGCACATCT CTGAGCTCCG GCGGGAAGGC 
CGTGTGGCCA ATGTGGCTGA CGTGGTGAGC AAAGGCCAGA GGGTCAAGGT CAAAGTGCTG 
TCCTTCACTG GGACCAAAAC CAGCTTGAGC ATGAAGGATG TGGATCAAGA GACTGGAGAA 
GATCTAAACC CAAATCGACG GCGGAATCTT GTCGGGGAGA CCAACGAAGA GACCTCTATG 
AGGAATCCGG ACAGGCCTAC CCACCTGTCC CTCGTCAGTG CCCCTGAAGT AGAGGATGAC 
TCACTAGAGC GCAAGCGCCT TACCCGCATC TCTGACCCAG AGAAGTGGGA GATCAAGCAG 
ATGATTGCTG CCAATGTCCT TTCCAAGGAA GAGTTCCCAG ACTTCGATGA AGAGACTGGC 
ATTCTCCCTA AAGTGGATGA TGAAGAAGAT GAGGACCTTG AGATCGAGCT GGTTGAGGAA 
GAGCCTCCAT TCCTGAGAGG GCACACCAAG CAGAGCATGG ACATGAGCCC TATCAAAATC 
GTTAAGAATC CAGATGGCTC CCTCTCCCAA GCAGCAATGA TGCAGAGTGC CTTGGCCAAA 
GAAAGGCGGG AACTTAAGCA AGCACAGCGG GAAGCTGAGA TGGATTCTAT TCCCATGGGA 
CTCAACAAAC ATTGGGTTGA TCCGCTGCCT GATGCGGAAG GCAGGCAGAT TGCTGCCAAC 
ATGAGGGGTA TTGGGATGAT GCCCAATGAC ATTCCTGAAT GGAAGAAGCA TGCCTTTGGG 
GGCAACAAAG CTTCATATGG AAAAAAGACC CAGATGTCAA TCATTGAACA AAGGGAGAGC 
CTCCCCATCT ACAAGCTGAA GGAGCAACTG GTCCAGGCTG TCCATGACAA CCAGATCCTG 
ATTGTTATTG GAGAGACCGG GTCTGGGAAG ACCACGCAGA TCACCCAGTA CCTGGCAGAG 
GCAGGCTACA CTTCCAGGGG CAAGATTGGA TGTACCCAGC CTAGAAGAGT AGCGGCCATG 
TCTGTGGCCA AAAGAGTGTC AGAGGAGTTC GGTTGTTGCT TAGGCCAAGA GGTGGGCTAC 
ACCATTCGAT TTGAGGACTG CACCAGCCCT GAAACAGTCA TCAAGTACAT GACAGACGGG 
ATGTTGCTCC GAGAATGCCT GATCGACCCC GACCTCACTC AGTATGCAAT CATCATGTTG 
GACGAGGCGC ACGAGAGGAC AATTCATACT GATGTGCTCT TTGGATTGCT GAAAAAGACG 
GTTCAGAAAC GGCAGGACAT GAAACTGATT GTCACCTCAG CCACCTTGGA TGCTGTGAAG 
TTTTCTCAGT ACTTCTATGA AGCTCCCATC TTTACCATCC CAGGTCGAAC ATACCCAGTG 
GAAATATTGT ACACAAAGGA ACCTGAAACC GATTATCTAG ATGCCAGCTT GATCACAGTC 
ATGCAGATCC ACTTAACAGA ACCGCCAGGT GATATCCTGG TCTTCCTGAC TGGTCAGGAA 
GAGATCGATA CTGCTTGCGA GATTCTGTAT GAAAGAATGA AATCCCTGGG ACCTGATGTT 
CCAGAGCTGA TTATCCTCCC AGTGTACTCT GCTCTCCCCA GTGAGATGCA GACCAGAATC 
TTTGACCCAG CTCCACCTGG CAGCAGAAAG GTTGTGATTG CCACCAACAT TGCAGAGACA 
TCGCTGACTA TTGATGGCAT TTACTATGTG GTAGACCCTG GATTCGTGAA GCAGAAGGTG 
TACAATTCTA AGACAGGGAT TGACCAGCTC GTGGTGACTC CTATTTCTCA GGCTCAGGCA 
AAGCAACGAG CTGGCAGAGC TGGGAGAACA GGCCCAGGGA AGTGTTATAG GCTGTACACA 
GAACGTGCCT ACCGAGATGA AATGCTGACC ACCAACGTGC CAGAAATTCA GAGAACCAAC 
TTAGCAAGCA CAGTGCTCTC GCTCAAGGCC ATGGGCATCA ATGACCTGCT GTCGTTTGAC 
TTCATGGATG CCCCGCCAAT GGAAACCTTG ATCACAGCCA TGGAGCAGCT GTACACGCTG 
GGGGCCCTGG ACGACGAGGG CCTGCTTACT CGCCTGGGCC GCAGGATGGC AGAGTTCCCT 
CTGGAGCCAA TGCTGTGCAA AATGCTGATC ATGTCTGTGC ATCTAGGCTG CAGTGAGGAA 
ATGCTGACCA TTGTGTCCAT GCTGTCTGTG CAGAACGTCT TCTACAGGCC CAAAGATAAA 
CAAGCCCTTG CAGATCAGAA GAAGGCCAAA TTCCACCAGA CCGAAGGGGA CCACCTCACC 
CTGCTGGCCG TTTACAACTC TTGGAAGAAC AACAAGTTCT CCAACCCATG GTGCTATGAG 
AACTTTATCC AGGCTCGTTC TCTGCGCCGG GCCCAGGACA TTCGCAAGCA GATGTTGGGC 
ATAATGGACA GACACAAGCT GGATGTGGTG TCCTGTGGTA AGTCCACAGT CCGAGTGCAG 
AAGGCCATCT GCAGTGGATT CTTCCGGAAT GCTGCCAAGA AAGACCCACA GGAGGGTTAC 
CGGACACTGA TTGACCAGCA AGTGGTCTAC ATCCATCCGT CCAGTGCTCT CTTCAACAGA 
CAGCCCGAAT GGGTGGTATA TCATGAGCTG GTGCTGACTA CGAAGGAGTA CATGCGTGAG 
GTCACCACCA TCGACCCCCG GTGGCTTGTG GAGTTTGCAC CGGCCTTCTT CAAGGTCTCT 
GACCCGACCA AGCTAAGCAA GCAGAAGAAG CAGCAACGGC TGGAACCCTT GTACAACCGC 
TATGAGGAGC CCAATGCCTG GAGAATATCC CGAGCCTTCC GGCGACGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012041559.1
CDS	1..3651
Translation	MAVAVAMAGAVPGTELGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKHRDTEHRDKAKKKKRSRSRERDRDRERERDRDHKRRHRSRSRSRSRTRDRNKGKSRYRSRSRSQSPPRDRKDRDKYGERNLDRWRDKHVDRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIIEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_012186169.2
Organism	Ovis aries(sheep)
Definition	Ovis aries DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012186169.2

ORF Insert Sequence:

ATGGCGGTGG CTGTGGCCAT GGCAGGAGCC GTACCCGGGA CAGAGCTAGG CCCCGCGGAA 
GAACTCGCCA AACTCGAGTA TCTGTCTTTG GTGTCAAAGG TTTGCACCGA GCTGGACAAT 
CACTTAGGTA TCAACGACAA GGATTTGGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTCAA GGCTTCGCTG GTCAAAAATG GTGCAGAATT TACGGATTCA 
CTCATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCAAA GCCTTCTACT 
AGCAAAGATC CAGTTGTTAA GCCCAAGACT GAAAAAGAAA AGCTGAAGGA GCTCTTCCCA 
GTCCTTTGCC AGCCAGACAA CCCTTCTGTT CGGACCATGC TGGACGAGGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTAAA AGAACTGGAA GCCTTGATGC CCAGTGCAGC AGGCCAGGAG 
AAGCACAGAG ACACCGAGCA CCGGGACAAG GCCAAGAAAA AGAAGCGCAG CCGGAGCCGA 
GAGCGAGACC GTGACCGCGA GCGAGAACGT GATAGAGACC ATAAGCGGAG ACACCGGTCC 
CGCTCTCGAT CACGTTCCCG GACCCGGGAC AGGAATAAAG GGAAGTCTAG ATACCGGTCC 
AGGAGCAGAA GTCAAAGTCC CCCCAGAGAT CGGAAAGACC GAGACAAGTA TGGGGAGCGG 
AATCTGGACA GATGGCGGGA TAAACACGTG GACCGCCCTC CCCCAGAAGA ACCCGCCATT 
GGGGACATTT ACAACGGCAA GGTGACCAGC ATCATGCAGT TTGGATGTTT CGTGCAGCTG 
GAGGGACTGA GGAAGCGGTG GGAAGGCCTG GTGCACATCT CTGAGCTCCG GCGGGAAGGC 
CGTGTGGCCA ATGTGGCTGA CGTGGTGAGC AAAGGCCAGA GGGTCAAGGT CAAAGTGCTG 
TCCTTCACTG GGACCAAAAC CAGCTTGAGC ATGAAGGATG TGGATCAAGA GACTGGAGAA 
GATCTAAACC CAAATCGACG GCGGAATCTT GTCGGGGAGA CCAACGAAGA GACCTCTATG 
AGGAATCCGG ACAGGCCTAC CCACCTGTCC CTCGTCAGTG CCCCTGAAGT AGAGGATGAC 
TCACTAGAGC GCAAGCGCCT TACCCGCATC TCTGACCCAG AGAAGTGGGA GATCAAGCAG 
ATGATTGCTG CCAATGTCCT TTCCAAGGAA GAGTTCCCAG ACTTCGATGA AGAGACTGGC 
ATTCTCCCTA AAGTGGATGA TGAAGAAGAT GAGGACCTTG AGATCGAGCT GGTTGAGGAA 
GAGCCTCCAT TCCTGAGAGG GCACACCAAG CAGAGCATGG ACATGAGCCC TATCAAAATC 
GTTAAGAATC CAGATGGCTC CCTCTCCCAA GCAGCAATGA TGCAGAGTGC CTTGGCCAAA 
GAAAGGCGGG AACTTAAGCA AGCACAGCGG GAAGCTGAGA TGGATTCTAT TCCCATGGGA 
CTCAACAAAC ATTGGGTTGA TCCGCTGCCT GATGCGGAAG GCAGGCAGAT TGCTGCCAAC 
ATGAGGGGTA TTGGGATGAT GCCCAATGAC ATTCCTGAAT GGAAGAAGCA TGCCTTTGGG 
GGCAACAAAG CTTCATATGG AAAAAAGACC CAGATGTCAA TCATTGAACA AAGGGAGAGC 
CTCCCCATCT ACAAGCTGAA GGAGCAACTG GTCCAGGCTG TCCATGACAA CCAGATCCTG 
ATTGTTATTG GAGAGACCGG GTCTGGGAAG ACCACGCAGA TCACCCAGTA CCTGGCAGAG 
GCAGGCTACA CTTCCAGGGG CAAGATTGGA TGTACCCAGC CTAGAAGAGT AGCGGCCATG 
TCTGTGGCCA AAAGAGTGTC AGAGGAGTTC GGTTGTTGCT TAGGCCAAGA GGTGGGCTAC 
ACCATTCGAT TTGAGGACTG CACCAGCCCT GAAACAGTCA TCAAGTACAT GACAGACGGG 
ATGTTGCTCC GAGAATGCCT GATCGACCCC GACCTCACTC AGTATGCAAT CATCATGTTG 
GACGAGGCGC ACGAGAGGAC AATTCATACT GATGTGCTCT TTGGATTGCT GAAAAAGACG 
GTTCAGAAAC GGCAGGACAT GAAACTGATT GTCACCTCAG CCACCTTGGA TGCTGTGAAG 
TTTTCTCAGT ACTTCTATGA AGCTCCCATC TTTACCATCC CAGGTCGAAC ATACCCAGTG 
GAAATATTGT ACACAAAGGA ACCTGAAACC GATTATCTAG ATGCCAGCTT GATCACAGTC 
ATGCAGATCC ACTTAACAGA ACCGCCAGGT GATATCCTGG TCTTCCTGAC TGGTCAGGAA 
GAGATCGATA CTGCTTGCGA GATTCTGTAT GAAAGAATGA AATCCCTGGG ACCTGATGTT 
CCAGAGCTGA TTATCCTCCC AGTGTACTCT GCTCTCCCCA GTGAGATGCA GACCAGAATC 
TTTGACCCAG CTCCACCTGG CAGCAGAAAG GTTGTGATTG CCACCAACAT TGCAGAGACA 
TCGCTGACTA TTGATGGCAT TTACTATGTG GTAGACCCTG GATTCGTGAA GCAGAAGGTG 
TACAATTCTA AGACAGGGAT TGACCAGCTC GTGGTGACTC CTATTTCTCA GGCTCAGGCA 
AAGCAACGAG CTGGCAGAGC TGGGAGAACA GGCCCAGGGA AGTGTTATAG GCTGTACACA 
GAACGTGCCT ACCGAGATGA AATGCTGACC ACCAACGTGC CAGAAATTCA GAGAACCAAC 
TTAGCAAGCA CAGTGCTCTC GCTCAAGGCC ATGGGCATCA ATGACCTGCT GTCGTTTGAC 
TTCATGGATG CCCCGCCAAT GGAAACCTTG ATCACAGCCA TGGAGCAGCT GTACACGCTG 
GGGGCCCTGG ACGACGAGGG CCTGCTTACT CGCCTGGGCC GCAGGATGGC AGAGTTCCCT 
CTGGAGCCAA TGCTGTGCAA AATGCTGATC ATGTCTGTGC ATCTAGGCTG CAGTGAGGAA 
ATGCTGACCA TTGTGTCCAT GCTGTCTGTG CAGAACGTCT TCTACAGGCC CAAAGATAAA 
CAAGCCCTTG CAGATCAGAA GAAGGCCAAA TTCCACCAGA CCGAAGGGGA CCACCTCACC 
CTGCTGGCCG TTTACAACTC TTGGAAGAAC AACAAGTTCT CCAACCCATG GTGCTATGAG 
AACTTTATCC AGGCTCGTTC TCTGCGCCGG GCCCAGGACA TTCGCAAGCA GATGTTGGGC 
ATAATGGACA GACACAAGCT GGATGTGGTG TCCTGTGGTA AGTCCACAGT CCGAGTGCAG 
AAGGCCATCT GCAGTGGATT CTTCCGGAAT GCTGCCAAGA AAGACCCACA GGAGGGTTAC 
CGGACACTGA TTGACCAGCA AGTGGTCTAC ATCCATCCGT CCAGTGCTCT CTTCAACAGA 
CAGCCCGAAT GGGTGGTATA TCATGAGCTG GTGCTGACTA CGAAGGAGTA CATGCGTGAG 
GTCACCACCA TCGACCCCCG GTGGCTTGTG GAGTTTGCAC CGGCCTTCTT CAAGGTCTCT 
GACCCGACCA AGCTAAGCAA GCAGAAGAAG CAGCAACGGC TGGAACCCTT GTACAACCGC 
TATGAGGAGC CCAATGCCTG GAGAATATCC CGAGCCTTCC GGCGACGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( XM_027974463.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027830264.1 Ovis aries DEAH-box helicase 8 (DHX8), transcript variant X2, mRNA.

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CloneID	OSm60328
Clone ID Related Accession (Same CDS sequence)	XM_027974463.1
Accession Version	XM_027974463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	ATP-dependent RNA helicase DHX8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG CTGTGGCCAT GGCAGGAGCC GTACCCGGGA CAGAGCTAGG CCCCGCGGAA 
GAACTCGCCA AACTCGAGTA TCTGTCTTTG GTGTCAAAGG TTTGCACCGA GCTGGACAAT 
CACTTAGGTA TCAACGACAA GGATTTGGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTCAA GGCTTCGCTG GTCAAAAATG GTGCAGAATT TACGGATTCA 
CTCATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCAAA GCCTTCTACT 
AGCAAAGATC CAGTTGTTAA GCCCAAGACT GAAAAAGAAA AGCTGAAGGA GCTCTTCCCA 
GTCCTTTGCC AGCCAGACAA CCCTTCTGTT CGGACCATGC TGGACGAGGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTAAA AGAACTGGAA GCCTTGATGC CCAGTGCAGC AGGCCAGGAG 
AAGCACAGAG ACACCGAGCA CCGGGATGTC AGCCAAAAAA AGAAGCGCAG CCGGAGCCGA 
GAGCGAGACC GTGACCGCGA GCGAGAACGT GATAGAGACC ATAAGCGGAG ACACCGGTCC 
CGCTCTCGAT CACGTTCCCG GACCCGGGAC AGGAATAAAG GGAAGTCTAG ATACCGGTCC 
AGGAGCAGAA GTCAAAGTCC CCCCAGAGAT CGGAAAGACC GAGACAAGTA TGGGGAGCGG 
AATCTGGACA GATGGCGGGA TAAACACGTG GACCGCCCTC CCCCAGAAGA ACCCGCCATT 
GGGGACATTT ACAACGGCAA GGTGACCAGC ATCATGCAGT TTGGATGTTT CGTGCAGCTG 
GAGGGACTGA GGAAGCGGTG GGAAGGCCTG GTGCACATCT CTGAGCTCCG GCGGGAAGGC 
CGTGTGGCCA ATGTGGCTGA TGTGGTGAGC AAAGGCCAGA GGGTCAAGGT CAAAGTGCTG 
TCCTTCACTG GGACCAAAAC CAGCTTGAGC ATGAAGGATG TGGATCAAGA GACTGGAGAA 
GATCTAAACC CAAATCGACG GCGGAATCTT GTCGGGGAGA CCAACGAAGA GACCTCTATG 
AGGAATCCGG ACAGGCCTAC CCACCTGTCC CTCGTCAGTG CCCCTGAAGT AGAGGATGAC 
TCACTAGAGC GCAAGCGCCT TACCCGCATC TCTGACCCAG AGAAGTGGGA GATCAAGCAG 
ATGATTGCTG CCAATGTCCT TTCCAAGGAA GAGTTCCCAG ACTTCGATGA AGAGACTGGC 
ATTCTCCCTA AAGTGGATGA TGAAGAAGAT GAGGACCTTG AGATTGAGCT GGTTGAGGAA 
GAGCCTCCAT TCCTGAGAGG GCACACCAAG CAGAGCATGG ACATGAGCCC TATCAAAATC 
GTTAAGAATC CAGATGGCTC CCTCTCCCAA GCAGCAATGA TGCAGAGTGC CTTGGCCAAA 
GAAAGGCGGG AACTTAAGCA AGCACAGCGG GAAGCTGAGA TGGATTCTAT TCCCATGGGA 
CTCAACAAAC ATTGGGTTGA TCCGCTGCCT GATGCGGAAG GCAGGCAGAT TGCTGCCAAC 
ATGAGGGGTA TTGGGATGAT GCCCAATGAC ATTCCTGAAT GGAAGAAGCA TGCCTTTGGG 
GGCAACAAAG CTTCATATGG AAAAAAGACC CAGATGTCAA TCATTGAACA AAGGGAGAGC 
CTCCCCATCT ACAAGCTGAA GGAGCAACTG GTCCAGGCTG TCCATGACAA CCAGATCCTG 
ATTGTTATTG GAGAGACCGG GTCTGGGAAG ACCACGCAGA TCACCCAGTA CCTGGCAGAG 
GCAGGCTACA CTTCCAGGGG CAAGATTGGA TGTACCCAGC CTAGAAGAGT AGCGGCCATG 
TCTGTGGCCA AAAGAGTGTC AGAGGAGTTC GGTTGTTGCT TAGGCCAAGA GGTGGGCTAC 
ACCATTCGAT TTGAGGACTG CACCAGCCCT GAAACAGTCA TCAAGTACAT GACAGACGGG 
ATGTTGCTCC GAGAATGCCT GATCGACCCC GACCTCACTC AGTATGCAAT CATCATGTTG 
GACGAGGCGC ATGAGAGGAC AATTCATACT GATGTGCTCT TTGGATTGCT GAAAAAGACG 
GTTCAGAAAC GGCAGGACAT GAAACTGATT GTCACCTCAG CCACCTTGGA TGCTGTGAAG 
TTTTCTCAGT ACTTCTATGA AGCTCCCATC TTTACCATCC CAGGTCGAAC ATACCCAGTG 
GAAATATTGT ACACAAAGGA ACCTGAAACC GATTATCTAG ATGCCAGCTT GATCACAGTC 
ATGCAGATCC ACTTAACAGA ACCGCCAGGT GATATCCTGG TCTTCCTGAC TGGTCAGGAA 
GAGATCGATA CTGCTTGCGA GATTCTGTAT GAAAGAATGA AATCCCTGGG ACCTGATGTT 
CCAGAGCTGA TTATCCTCCC AGTGTACTCT GCTCTCCCCA GTGAGATGCA GACCAGAATC 
TTTGACCCAG CTCCACCTGG CAGCAGAAAG GTTGTGATTG CCACCAACAT TGCAGAGACA 
TCGCTGACTA TTGATGGCAT TTACTATGTG GTAGACCCTG GATTCGTGAA GCAGAAGGTG 
TACAATTCTA AGACAGGGAT TGACCAGCTC GTGGTGACTC CTATTTCTCA GGCTCAGGCA 
AAGCAACGAG CTGGCAGAGC TGGGAGAACA GGCCCAGGGA AGTGTTATAG GCTGTACACA 
GAACGTGCCT ACCGAGATGA AATGCTGACC ACCAATGTGC CAGAAATTCA GAGAACCAAC 
TTAGCAAGCA CAGTGCTCTC GCTCAAGGCC ATGGGCATCA ATGACCTGCT GTCGTTTGAC 
TTCATGGATG CCCCGCCAAT GGAAACCTTG ATCACAGCCA TGGAGCAGCT GTACACGCTG 
GGGGCCCTGG ACGACGAGGG CCTGCTTACT CGCCTGGGCC GCAGGATGGC AGAGTTCCCT 
CTGGAGCCAA TGCTGTGCAA AATGCTGATC ATGTCTGTGC ATCTAGGCTG CAGTGAGGAA 
ATGCTGACCA TTGTGTCCAT GCTGTCTGTG CAGAACGTCT TCTACAGGCC CAAAGATAAA 
CAAGCCCTTG CAGATCAGAA GAAGGCCAAA TTCCACCAGA CCGAAGGGGA CCACCTCACC 
CTGCTGGCCG TTTACAACTC TTGGAAGAAC AACAAGTTCT CCAACCCATG GTGCTATGAG 
AACTTTATCC AGGCTCGTTC TCTGCGCCGG GCCCAGGACA TTCGCAAGCA GATGTTGGGC 
ATAATGGACA GACACAAGCT GGATGTGGTG TCCTGTGGTA AGTCCACAGT CCGAGTGCAG 
AAGGCCATCT GCAGTGGATT CTTCCGGAAT GCTGCCAAGA AAGACCCACA GGAGGGTTAC 
CGGACACTGA TTGACCAGCA AGTGGTCTAC ATCCATCCGT CCAGTGCTCT CTTCAACAGA 
CAGCCCGAAT GGGTGGTATA TCATGAGCTG GTGCTGACTA CGAAGGAGTA CATGCGTGAG 
GTCACCACCA TCGACCCCCG GTGGCTTGTG GAGTTTGCAC CGGCCTTCTT CAAGGTCTCT 
GACCCGACCA AGCTAAGCAA GCAGAAGAAG CAGCAACGGC TGGAACCCTT GTACAACCGC 
TATGAGGAGC CCAATGCCTG GAGAATATCC CGAGCCTTCC GGCGACGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027830264.1
CDS	86..3736
Translation	MAVAVAMAGAVPGTELGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKHRDTEHRDVSQKKKRSRSRERDRDRERERDRDHKRRHRSRSRSRSRTRDRNKGKSRYRSRSRSQSPPRDRKDRDKYGERNLDRWRDKHVDRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIIEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_027974463.1
Organism	Ovis aries(sheep)
Definition	Ovis aries DEAH-box helicase 8 (DHX8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027974463.1

ORF Insert Sequence:

ATGGCGGTGG CTGTGGCCAT GGCAGGAGCC GTACCCGGGA CAGAGCTAGG CCCCGCGGAA 
GAACTCGCCA AACTCGAGTA TCTGTCTTTG GTGTCAAAGG TTTGCACCGA GCTGGACAAT 
CACTTAGGTA TCAACGACAA GGATTTGGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTCAA GGCTTCGCTG GTCAAAAATG GTGCAGAATT TACGGATTCA 
CTCATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCAAA GCCTTCTACT 
AGCAAAGATC CAGTTGTTAA GCCCAAGACT GAAAAAGAAA AGCTGAAGGA GCTCTTCCCA 
GTCCTTTGCC AGCCAGACAA CCCTTCTGTT CGGACCATGC TGGACGAGGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTAAA AGAACTGGAA GCCTTGATGC CCAGTGCAGC AGGCCAGGAG 
AAGCACAGAG ACACCGAGCA CCGGGATGTC AGCCAAAAAA AGAAGCGCAG CCGGAGCCGA 
GAGCGAGACC GTGACCGCGA GCGAGAACGT GATAGAGACC ATAAGCGGAG ACACCGGTCC 
CGCTCTCGAT CACGTTCCCG GACCCGGGAC AGGAATAAAG GGAAGTCTAG ATACCGGTCC 
AGGAGCAGAA GTCAAAGTCC CCCCAGAGAT CGGAAAGACC GAGACAAGTA TGGGGAGCGG 
AATCTGGACA GATGGCGGGA TAAACACGTG GACCGCCCTC CCCCAGAAGA ACCCGCCATT 
GGGGACATTT ACAACGGCAA GGTGACCAGC ATCATGCAGT TTGGATGTTT CGTGCAGCTG 
GAGGGACTGA GGAAGCGGTG GGAAGGCCTG GTGCACATCT CTGAGCTCCG GCGGGAAGGC 
CGTGTGGCCA ATGTGGCTGA TGTGGTGAGC AAAGGCCAGA GGGTCAAGGT CAAAGTGCTG 
TCCTTCACTG GGACCAAAAC CAGCTTGAGC ATGAAGGATG TGGATCAAGA GACTGGAGAA 
GATCTAAACC CAAATCGACG GCGGAATCTT GTCGGGGAGA CCAACGAAGA GACCTCTATG 
AGGAATCCGG ACAGGCCTAC CCACCTGTCC CTCGTCAGTG CCCCTGAAGT AGAGGATGAC 
TCACTAGAGC GCAAGCGCCT TACCCGCATC TCTGACCCAG AGAAGTGGGA GATCAAGCAG 
ATGATTGCTG CCAATGTCCT TTCCAAGGAA GAGTTCCCAG ACTTCGATGA AGAGACTGGC 
ATTCTCCCTA AAGTGGATGA TGAAGAAGAT GAGGACCTTG AGATTGAGCT GGTTGAGGAA 
GAGCCTCCAT TCCTGAGAGG GCACACCAAG CAGAGCATGG ACATGAGCCC TATCAAAATC 
GTTAAGAATC CAGATGGCTC CCTCTCCCAA GCAGCAATGA TGCAGAGTGC CTTGGCCAAA 
GAAAGGCGGG AACTTAAGCA AGCACAGCGG GAAGCTGAGA TGGATTCTAT TCCCATGGGA 
CTCAACAAAC ATTGGGTTGA TCCGCTGCCT GATGCGGAAG GCAGGCAGAT TGCTGCCAAC 
ATGAGGGGTA TTGGGATGAT GCCCAATGAC ATTCCTGAAT GGAAGAAGCA TGCCTTTGGG 
GGCAACAAAG CTTCATATGG AAAAAAGACC CAGATGTCAA TCATTGAACA AAGGGAGAGC 
CTCCCCATCT ACAAGCTGAA GGAGCAACTG GTCCAGGCTG TCCATGACAA CCAGATCCTG 
ATTGTTATTG GAGAGACCGG GTCTGGGAAG ACCACGCAGA TCACCCAGTA CCTGGCAGAG 
GCAGGCTACA CTTCCAGGGG CAAGATTGGA TGTACCCAGC CTAGAAGAGT AGCGGCCATG 
TCTGTGGCCA AAAGAGTGTC AGAGGAGTTC GGTTGTTGCT TAGGCCAAGA GGTGGGCTAC 
ACCATTCGAT TTGAGGACTG CACCAGCCCT GAAACAGTCA TCAAGTACAT GACAGACGGG 
ATGTTGCTCC GAGAATGCCT GATCGACCCC GACCTCACTC AGTATGCAAT CATCATGTTG 
GACGAGGCGC ATGAGAGGAC AATTCATACT GATGTGCTCT TTGGATTGCT GAAAAAGACG 
GTTCAGAAAC GGCAGGACAT GAAACTGATT GTCACCTCAG CCACCTTGGA TGCTGTGAAG 
TTTTCTCAGT ACTTCTATGA AGCTCCCATC TTTACCATCC CAGGTCGAAC ATACCCAGTG 
GAAATATTGT ACACAAAGGA ACCTGAAACC GATTATCTAG ATGCCAGCTT GATCACAGTC 
ATGCAGATCC ACTTAACAGA ACCGCCAGGT GATATCCTGG TCTTCCTGAC TGGTCAGGAA 
GAGATCGATA CTGCTTGCGA GATTCTGTAT GAAAGAATGA AATCCCTGGG ACCTGATGTT 
CCAGAGCTGA TTATCCTCCC AGTGTACTCT GCTCTCCCCA GTGAGATGCA GACCAGAATC 
TTTGACCCAG CTCCACCTGG CAGCAGAAAG GTTGTGATTG CCACCAACAT TGCAGAGACA 
TCGCTGACTA TTGATGGCAT TTACTATGTG GTAGACCCTG GATTCGTGAA GCAGAAGGTG 
TACAATTCTA AGACAGGGAT TGACCAGCTC GTGGTGACTC CTATTTCTCA GGCTCAGGCA 
AAGCAACGAG CTGGCAGAGC TGGGAGAACA GGCCCAGGGA AGTGTTATAG GCTGTACACA 
GAACGTGCCT ACCGAGATGA AATGCTGACC ACCAATGTGC CAGAAATTCA GAGAACCAAC 
TTAGCAAGCA CAGTGCTCTC GCTCAAGGCC ATGGGCATCA ATGACCTGCT GTCGTTTGAC 
TTCATGGATG CCCCGCCAAT GGAAACCTTG ATCACAGCCA TGGAGCAGCT GTACACGCTG 
GGGGCCCTGG ACGACGAGGG CCTGCTTACT CGCCTGGGCC GCAGGATGGC AGAGTTCCCT 
CTGGAGCCAA TGCTGTGCAA AATGCTGATC ATGTCTGTGC ATCTAGGCTG CAGTGAGGAA 
ATGCTGACCA TTGTGTCCAT GCTGTCTGTG CAGAACGTCT TCTACAGGCC CAAAGATAAA 
CAAGCCCTTG CAGATCAGAA GAAGGCCAAA TTCCACCAGA CCGAAGGGGA CCACCTCACC 
CTGCTGGCCG TTTACAACTC TTGGAAGAAC AACAAGTTCT CCAACCCATG GTGCTATGAG 
AACTTTATCC AGGCTCGTTC TCTGCGCCGG GCCCAGGACA TTCGCAAGCA GATGTTGGGC 
ATAATGGACA GACACAAGCT GGATGTGGTG TCCTGTGGTA AGTCCACAGT CCGAGTGCAG 
AAGGCCATCT GCAGTGGATT CTTCCGGAAT GCTGCCAAGA AAGACCCACA GGAGGGTTAC 
CGGACACTGA TTGACCAGCA AGTGGTCTAC ATCCATCCGT CCAGTGCTCT CTTCAACAGA 
CAGCCCGAAT GGGTGGTATA TCATGAGCTG GTGCTGACTA CGAAGGAGTA CATGCGTGAG 
GTCACCACCA TCGACCCCCG GTGGCTTGTG GAGTTTGCAC CGGCCTTCTT CAAGGTCTCT 
GACCCGACCA AGCTAAGCAA GCAGAAGAAG CAGCAACGGC TGGAACCCTT GTACAACCGC 
TATGAGGAGC CCAATGCCTG GAGAATATCC CGAGCCTTCC GGCGACGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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DHX8 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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