THBS1 gene cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

mRNA	Protein	Name
XM_012131911.2	XP_011987301.1	thrombospondin-1 isoform X3
XM_015096931.1	XP_014952417.1	thrombospondin-1 isoform X2
XM_012131909.1	XP_011987299.1	thrombospondin-1 isoform X1
XM_012131910.1	XP_011987300.1	thrombospondin-1 isoform X2
XM_015096932.1	XP_014952418.1	thrombospondin-1 isoform X4
XM_027971666.1	XP_027827467.1	thrombospondin-1 isoform X4
XM_027971665.1	XP_027827466.1	thrombospondin-1 isoform X3
XM_027971664.1	XP_027827465.1	thrombospondin-1 isoform X2
XM_027971663.1	XP_027827464.1	thrombospondin-1 isoform X1
XM_015096929.1	XP_014952415.1	thrombospondin-1 isoform X3
XM_015096930.1	XP_014952416.1	thrombospondin-1 isoform X1
XM_012131912.2	XP_011987302.1	thrombospondin-1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv14779	XM_012131909.1 Latest version!	Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOv14780	XM_012131910.1 Latest version!	Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOv14781	XM_012131911.2 Latest version!	Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOv14782	XM_012131912.2 Latest version!	Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm07823	XM_015096932.1 Latest version!	Ovis aries thrombospondin 1 (THBS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm27270	XM_015096930.1 Latest version!	Ovis aries thrombospondin 1 (THBS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm27271	XM_015096931.1 Latest version!	Ovis aries thrombospondin 1 (THBS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm27272	XM_015096929.1 Latest version!	Ovis aries thrombospondin 1 (THBS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm82786	XM_027971663.1 Latest version!	Ovis aries thrombospondin 1 (THBS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm82787	XM_027971664.1 Latest version!	Ovis aries thrombospondin 1 (THBS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm82788	XM_027971665.1 Latest version!	Ovis aries thrombospondin 1 (THBS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm82789	XM_027971666.1 Latest version!	Ovis aries thrombospondin 1 (THBS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

THBS1 ( XM_012131909.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011987299.1 Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOv14779
Clone ID Related Accession (Same CDS sequence)	XM_012131909.1
Accession Version	XM_012131909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	thrombospondin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942376.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAACGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGATG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCCGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC TGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011987299.1
CDS	163..3678
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSATSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_012131909.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012131909.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAACGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGATG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCCGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC TGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_012131910.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011987300.1 Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OOv14780
Clone ID Related Accession (Same CDS sequence)	XM_012131910.1
Accession Version	XM_012131910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	thrombospondin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942376.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CAGCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGATG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCCGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC TGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011987300.1
CDS	163..3678
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPASDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_012131910.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012131910.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CAGCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGATG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCCGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC TGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_012131911.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011987301.1 Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X7, mRNA.

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CloneID	OOv14781
Clone ID Related Accession (Same CDS sequence)	XM_012131911.2
Accession Version	XM_012131911.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	thrombospondin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942376.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012131911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAACGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCAGCCAG CGACTCTGCA 
GACGATGGCT GGTCCCCGTG GTCTGAGTGG ACCTCTTGCT CTGTGACCTG TGGCAATGGA 
ATCCAGCAGC GTGGCCGCTC CTGCGACAGC CTCAACAACA GATGCGAGGG CTCCTCTGTG 
CAGACGCGGA CCTGCCACAT CCAGGAGTGT GACAAGAGAT TTAAACAGGA TGGAGGCTGG 
AGCCACTGGT CCCCGTGGTC ATCTTGTTCC GTAACATGTG GAGATGGTGT GATCACAAGG 
ATCCGGCTCT GCAACTCCCC CAGCCCCCAG ATGAATGGGA AGCCATGTGA GGGCAAAGCC 
CGGGAGACCA AAGCCTGCCA GAAAGACGCC TGCCCCATCA ATGGAGGCTG GGGCCCCTGG 
TCACCATGGG ACATCTGTTC TGTCACCTGC GGAGGAGGGG CACAGAAACG TAGCCGGCTC 
TGCAACAACC CCACGCCCCA GTTTGGAGGC AAGGACTGCG TTGGTGATGT AACAGAAACC 
CAGATCTGCA ACAAGCAGGA CTGTCCCATT GATGGCTGCC TGTCCAATCC CTGCTTTGCT 
GGTGTCCAGT GTACCAGCTA CCCTGATGGC AGCTGGAAGT GTGGTGCTTG TCCCCCAGGC 
TATAGCGGAG ATGGCGTCAA GTGCGAAGAT GTTGATGAGT GCAAAGAAGT TCCTGATGCC 
TGCTTCAACC ACAATGGAGA GCACAGGTGT GAGAACACAG ACCCCGGCTA CAACTGCCTG 
CCCTGCCCAC CACGCTTCAC TGGCTCGCAG CCCTTCGGCC GGGGCGTGGA ACATGCCACC 
GCCAACAAAC AGGTATGCAA GCCCCGAAAC CCCTGCACGG ACGGGACACA TGACTGCAAC 
AAGAACGCCA AGTGCAACTA CCTGGGCCAC TACAGTGACC CCATGTACCG CTGCGAGTGC 
AAGCCTGGCT ACGCCGGCAA CGGCATCATC TGCGGGGAGG ACACAGACCT GGACGGCTGG 
CCCAACGAGG ACCTGCTGTG CGTGGCCAAT GCAACTTACC ACTGCAGAAA GGATAATTGC 
CCCAACCTTC CCAACTCAGG GCAGGAAGAC TATGACAAGG ATGGAATCGG CGATGCCTGT 
GATGATGACG ATGACAATGA TAAGATTCCA GATGACAGGG ACAACTGTCC ATTCCATTAC 
AACCCAGCCC AGTATGACTA TGACAGAGAT GACGTGGGAG ACCGCTGTGA CAACTGCCCC 
TACAACCACA ACCCAGACCA GGCTGACACA GATAACAATG GGGAAGGAGA CGCCTGCGCA 
GCTGACATTG ATGGGGACGG TATCCTCAAT GAACGGGACA ACTGCCAGTA TGTCTACAAT 
GTGGACCAGA AAGACACTGA CATGGACGGG GTTGGTGACC AGTGTGACAA CTGCCCCCTG 
GAACACAATC CAGACCAGCT CGACTCTGAC TCGGACCGCA TTGGAGACAC CTGTGACAAC 
AATCAGGATA TTGATGAAGA CGGCCACCAG AACAACCTGG ACAACTGTCC CTACGTGCCC 
AATGCCAACC AGGCCGACCA CGACAAGGAT GGCAAAGGCG ATGCCTGCGA CCACGATGAT 
GACAACGATG GCATTCCTGA TGACCGGGAC AACTGCAGGC TGGTGCCCAA TCCTGACCAG 
AAGGACTCTG ATGGTGATGG TCGAGGTGAT GCTTGCAAAG ATGATTTTGA CCAGGACAAG 
GTGCCAGACA TTGATGACAT CTGTCCCGAA AATGTTGATA TCAGTGAGAC TGATTTCCGC 
CGATTCCAGA TGATTCCTCT AGATCCCAAA GGGACATCCC AGAATGACCC TAACTGGGTT 
GTACGCCATC AGGGTAAAGA ACTCGTCCAG ACTGTCAACT GTGACCCCGG ACTTGCTGTA 
GGTTATGACG AATTTAATGC TGTGGACTTC AGCGGGACCT TCTTCATCAA CACTGAGAGG 
GATGACGACT ATGCCGGCTT TGTGTTTGGC TACCAGTCCA GCAGCCGCTT CTATGTTGTG 
ATGTGGAAGC AAGTCACTCA ATCCTATTGG GACACCAACC CCACGAGGGC TCAGGGATAC 
TCTGGACTTT CTGTGAAAGT TGTGAACTCC ACCACGGGGC CTGGCGAGCA CCTGCGGAAT 
GCCCTGTGGC ACACAGGAAA CACCCCTGGC CAGGTGCGCA CACTGTGGCA TGACCCTCGT 
CACATTGGCT GGAAAGATTT CACTGCCTAC AGATGGCGTC TGAGCCACAG GCCAAAGACA 
GGTTTCATCA GAGTGGTGAT GTATGAAGGG AAGAAAATCA TGGCTGACTC AGGACCCATC 
TATGACAAAA CCTATGCTGG TGGTAGGCTA GGCTTGTTCG TCTTCTCTCA AGAAATGGTG 
TTCTTCTCCG ACCTGAAATA TGAATGCAGA GATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011987301.1
CDS	195..3713
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSATSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPASDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_012131911.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012131911.2

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAACGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCAGCCAG CGACTCTGCA 
GACGATGGCT GGTCCCCGTG GTCTGAGTGG ACCTCTTGCT CTGTGACCTG TGGCAATGGA 
ATCCAGCAGC GTGGCCGCTC CTGCGACAGC CTCAACAACA GATGCGAGGG CTCCTCTGTG 
CAGACGCGGA CCTGCCACAT CCAGGAGTGT GACAAGAGAT TTAAACAGGA TGGAGGCTGG 
AGCCACTGGT CCCCGTGGTC ATCTTGTTCC GTAACATGTG GAGATGGTGT GATCACAAGG 
ATCCGGCTCT GCAACTCCCC CAGCCCCCAG ATGAATGGGA AGCCATGTGA GGGCAAAGCC 
CGGGAGACCA AAGCCTGCCA GAAAGACGCC TGCCCCATCA ATGGAGGCTG GGGCCCCTGG 
TCACCATGGG ACATCTGTTC TGTCACCTGC GGAGGAGGGG CACAGAAACG TAGCCGGCTC 
TGCAACAACC CCACGCCCCA GTTTGGAGGC AAGGACTGCG TTGGTGATGT AACAGAAACC 
CAGATCTGCA ACAAGCAGGA CTGTCCCATT GATGGCTGCC TGTCCAATCC CTGCTTTGCT 
GGTGTCCAGT GTACCAGCTA CCCTGATGGC AGCTGGAAGT GTGGTGCTTG TCCCCCAGGC 
TATAGCGGAG ATGGCGTCAA GTGCGAAGAT GTTGATGAGT GCAAAGAAGT TCCTGATGCC 
TGCTTCAACC ACAATGGAGA GCACAGGTGT GAGAACACAG ACCCCGGCTA CAACTGCCTG 
CCCTGCCCAC CACGCTTCAC TGGCTCGCAG CCCTTCGGCC GGGGCGTGGA ACATGCCACC 
GCCAACAAAC AGGTATGCAA GCCCCGAAAC CCCTGCACGG ACGGGACACA TGACTGCAAC 
AAGAACGCCA AGTGCAACTA CCTGGGCCAC TACAGTGACC CCATGTACCG CTGCGAGTGC 
AAGCCTGGCT ACGCCGGCAA CGGCATCATC TGCGGGGAGG ACACAGACCT GGACGGCTGG 
CCCAACGAGG ACCTGCTGTG CGTGGCCAAT GCAACTTACC ACTGCAGAAA GGATAATTGC 
CCCAACCTTC CCAACTCAGG GCAGGAAGAC TATGACAAGG ATGGAATCGG CGATGCCTGT 
GATGATGACG ATGACAATGA TAAGATTCCA GATGACAGGG ACAACTGTCC ATTCCATTAC 
AACCCAGCCC AGTATGACTA TGACAGAGAT GACGTGGGAG ACCGCTGTGA CAACTGCCCC 
TACAACCACA ACCCAGACCA GGCTGACACA GATAACAATG GGGAAGGAGA CGCCTGCGCA 
GCTGACATTG ATGGGGACGG TATCCTCAAT GAACGGGACA ACTGCCAGTA TGTCTACAAT 
GTGGACCAGA AAGACACTGA CATGGACGGG GTTGGTGACC AGTGTGACAA CTGCCCCCTG 
GAACACAATC CAGACCAGCT CGACTCTGAC TCGGACCGCA TTGGAGACAC CTGTGACAAC 
AATCAGGATA TTGATGAAGA CGGCCACCAG AACAACCTGG ACAACTGTCC CTACGTGCCC 
AATGCCAACC AGGCCGACCA CGACAAGGAT GGCAAAGGCG ATGCCTGCGA CCACGATGAT 
GACAACGATG GCATTCCTGA TGACCGGGAC AACTGCAGGC TGGTGCCCAA TCCTGACCAG 
AAGGACTCTG ATGGTGATGG TCGAGGTGAT GCTTGCAAAG ATGATTTTGA CCAGGACAAG 
GTGCCAGACA TTGATGACAT CTGTCCCGAA AATGTTGATA TCAGTGAGAC TGATTTCCGC 
CGATTCCAGA TGATTCCTCT AGATCCCAAA GGGACATCCC AGAATGACCC TAACTGGGTT 
GTACGCCATC AGGGTAAAGA ACTCGTCCAG ACTGTCAACT GTGACCCCGG ACTTGCTGTA 
GGTTATGACG AATTTAATGC TGTGGACTTC AGCGGGACCT TCTTCATCAA CACTGAGAGG 
GATGACGACT ATGCCGGCTT TGTGTTTGGC TACCAGTCCA GCAGCCGCTT CTATGTTGTG 
ATGTGGAAGC AAGTCACTCA ATCCTATTGG GACACCAACC CCACGAGGGC TCAGGGATAC 
TCTGGACTTT CTGTGAAAGT TGTGAACTCC ACCACGGGGC CTGGCGAGCA CCTGCGGAAT 
GCCCTGTGGC ACACAGGAAA CACCCCTGGC CAGGTGCGCA CACTGTGGCA TGACCCTCGT 
CACATTGGCT GGAAAGATTT CACTGCCTAC AGATGGCGTC TGAGCCACAG GCCAAAGACA 
GGTTTCATCA GAGTGGTGAT GTATGAAGGG AAGAAAATCA TGGCTGACTC AGGACCCATC 
TATGACAAAA CCTATGCTGG TGGTAGGCTA GGCTTGTTCG TCTTCTCTCA AGAAATGGTG 
TTCTTCTCCG ACCTGAAATA TGAATGCAGA GATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_012131912.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011987302.1 Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X8, mRNA.

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CloneID	OOv14782
Clone ID Related Accession (Same CDS sequence)	XM_012131912.2
Accession Version	XM_012131912.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	thrombospondin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942376.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012131912.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCAGACGAT 
GGCTGGTCCC CGTGGTCTGA GTGGACCTCT TGCTCTGTGA CCTGTGGCAA TGGAATCCAG 
CAGCGTGGCC GCTCCTGCGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC TGTGCAGACG 
CGGACCTGCC ACATCCAGGA GTGTGACAAG AGATTTAAAC AGGATGGAGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCCGTAACA TGTGGAGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CCCCCAGCCC CCAGATGAAT GGGAAGCCAT GTGAGGGCAA AGCCCGGGAG 
ACCAAAGCCT GCCAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGCCC CTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGCGGAGGA GGGGCACAGA AACGTAGCCG GCTCTGCAAC 
AACCCCACGC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AACCCAGATC 
TGCAACAAGC AGGACTGTCC CATTGATGGC TGCCTGTCCA ATCCCTGCTT TGCTGGTGTC 
CAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGTGGTG CTTGTCCCCC AGGCTATAGC 
GGAGATGGCG TCAAGTGCGA AGATGTTGAT GAGTGCAAAG AAGTTCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACAG GTGTGAGAAC ACAGACCCCG GCTACAACTG CCTGCCCTGC 
CCACCACGCT TCACTGGCTC GCAGCCCTTC GGCCGGGGCG TGGAACATGC CACCGCCAAC 
AAACAGGTAT GCAAGCCCCG AAACCCCTGC ACGGACGGGA CACATGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTACAGT GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCCG GCAACGGCAT CATCTGCGGG GAGGACACAG ACCTGGACGG CTGGCCCAAC 
GAGGACCTGC TGTGCGTGGC CAATGCAACT TACCACTGCA GAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGTGATGAT 
GACGATGACA ATGATAAGAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTATG ACTATGACAG AGATGACGTG GGAGACCGCT GTGACAACTG CCCCTACAAC 
CACAACCCAG ACCAGGCTGA CACAGATAAC AATGGGGAAG GAGACGCCTG CGCAGCTGAC 
ATTGATGGGG ACGGTATCCT CAATGAACGG GACAACTGCC AGTATGTCTA CAATGTGGAC 
CAGAAAGACA CTGACATGGA CGGGGTTGGT GACCAGTGTG ACAACTGCCC CCTGGAACAC 
AATCCAGACC AGCTCGACTC TGACTCGGAC CGCATTGGAG ACACCTGTGA CAACAATCAG 
GATATTGATG AAGACGGCCA CCAGAACAAC CTGGACAACT GTCCCTACGT GCCCAATGCC 
AACCAGGCCG ACCACGACAA GGATGGCAAA GGCGATGCCT GCGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACCG GGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC 
TCTGATGGTG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCAGGA CAAGGTGCCA 
GACATTGATG ACATCTGTCC CGAAAATGTT GATATCAGTG AGACTGATTT CCGCCGATTC 
CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CCGGACTTGC TGTAGGTTAT 
GACGAATTTA ATGCTGTGGA CTTCAGCGGG ACCTTCTTCA TCAACACTGA GAGGGATGAC 
GACTATGCCG GCTTTGTGTT TGGCTACCAG TCCAGCAGCC GCTTCTATGT TGTGATGTGG 
AAGCAAGTCA CTCAATCCTA TTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCTGGA 
CTTTCTGTGA AAGTTGTGAA CTCCACCACG GGGCCTGGCG AGCACCTGCG GAATGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACACTGT GGCATGACCC TCGTCACATT 
GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTGAGCC ACAGGCCAAA GACAGGTTTC 
ATCAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAC 
AAAACCTATG CTGGTGGTAG GCTAGGCTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCCGACCTGA AATATGAATG CAGAGATTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011987302.1
CDS	195..3707
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_012131912.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon thrombospondin 1 (THBS1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012131912.2

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCAGACGAT 
GGCTGGTCCC CGTGGTCTGA GTGGACCTCT TGCTCTGTGA CCTGTGGCAA TGGAATCCAG 
CAGCGTGGCC GCTCCTGCGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC TGTGCAGACG 
CGGACCTGCC ACATCCAGGA GTGTGACAAG AGATTTAAAC AGGATGGAGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCCGTAACA TGTGGAGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CCCCCAGCCC CCAGATGAAT GGGAAGCCAT GTGAGGGCAA AGCCCGGGAG 
ACCAAAGCCT GCCAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGCCC CTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGCGGAGGA GGGGCACAGA AACGTAGCCG GCTCTGCAAC 
AACCCCACGC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AACCCAGATC 
TGCAACAAGC AGGACTGTCC CATTGATGGC TGCCTGTCCA ATCCCTGCTT TGCTGGTGTC 
CAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGTGGTG CTTGTCCCCC AGGCTATAGC 
GGAGATGGCG TCAAGTGCGA AGATGTTGAT GAGTGCAAAG AAGTTCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACAG GTGTGAGAAC ACAGACCCCG GCTACAACTG CCTGCCCTGC 
CCACCACGCT TCACTGGCTC GCAGCCCTTC GGCCGGGGCG TGGAACATGC CACCGCCAAC 
AAACAGGTAT GCAAGCCCCG AAACCCCTGC ACGGACGGGA CACATGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTACAGT GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCCG GCAACGGCAT CATCTGCGGG GAGGACACAG ACCTGGACGG CTGGCCCAAC 
GAGGACCTGC TGTGCGTGGC CAATGCAACT TACCACTGCA GAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGTGATGAT 
GACGATGACA ATGATAAGAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTATG ACTATGACAG AGATGACGTG GGAGACCGCT GTGACAACTG CCCCTACAAC 
CACAACCCAG ACCAGGCTGA CACAGATAAC AATGGGGAAG GAGACGCCTG CGCAGCTGAC 
ATTGATGGGG ACGGTATCCT CAATGAACGG GACAACTGCC AGTATGTCTA CAATGTGGAC 
CAGAAAGACA CTGACATGGA CGGGGTTGGT GACCAGTGTG ACAACTGCCC CCTGGAACAC 
AATCCAGACC AGCTCGACTC TGACTCGGAC CGCATTGGAG ACACCTGTGA CAACAATCAG 
GATATTGATG AAGACGGCCA CCAGAACAAC CTGGACAACT GTCCCTACGT GCCCAATGCC 
AACCAGGCCG ACCACGACAA GGATGGCAAA GGCGATGCCT GCGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACCG GGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC 
TCTGATGGTG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCAGGA CAAGGTGCCA 
GACATTGATG ACATCTGTCC CGAAAATGTT GATATCAGTG AGACTGATTT CCGCCGATTC 
CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CCGGACTTGC TGTAGGTTAT 
GACGAATTTA ATGCTGTGGA CTTCAGCGGG ACCTTCTTCA TCAACACTGA GAGGGATGAC 
GACTATGCCG GCTTTGTGTT TGGCTACCAG TCCAGCAGCC GCTTCTATGT TGTGATGTGG 
AAGCAAGTCA CTCAATCCTA TTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCTGGA 
CTTTCTGTGA AAGTTGTGAA CTCCACCACG GGGCCTGGCG AGCACCTGCG GAATGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACACTGT GGCATGACCC TCGTCACATT 
GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTGAGCC ACAGGCCAAA GACAGGTTTC 
ATCAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAC 
AAAACCTATG CTGGTGGTAG GCTAGGCTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCCGACCTGA AATATGAATG CAGAGATTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_015096932.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952418.1 Ovis aries thrombospondin 1 (THBS1), transcript variant X4, mRNA.

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CloneID	OSm07823
Clone ID Related Accession (Same CDS sequence)	XM_015096932.1
Accession Version	XM_015096932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	thrombospondin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCAGACGAT 
GGCTGGTCCC CGTGGTCTGA GTGGACCTCT TGCTCTGTGA CCTGTGGCAA TGGAATCCAG 
CAGCGTGGCC GCTCCTGCGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC TGTGCAGACG 
CGGACCTGCC ACATCCAGGA GTGTGACAAG AGATTTAAAC AGGATGGAGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCCGTAACA TGTGGAGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CCCCCAGCCC CCAGATGAAT GGGAAGCCAT GTGAGGGCAA AGCCCGGGAG 
ACCAAAGCCT GCCAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGCCC CTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGCGGAGGA GGGGCACAGA AACGTAGCCG GCTCTGCAAC 
AACCCCACGC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AACCCAGATC 
TGCAACAAGC AGGACTGTCC CATTGATGGC TGCCTGTCCA ATCCCTGCTT TGCTGGTGTC 
CAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGTGGTG CTTGTCCCCC AGGCTATAGC 
GGAGATGGCG TCAAGTGCGA AGATGTTGAT GAGTGCAAAG AAGTTCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACAG GTGTGAGAAC ACAGACCCCG GCTACAACTG CCTGCCCTGC 
CCACCACGCT TCACTGGCTC GCAGCCCTTC GGCCGGGGCG TGGAACATGC CACCGCCAAC 
AAACAGGTAT GCAAGCCCCG AAACCCCTGC ACGGACGGGA CACATGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTACAGT GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCCG GCAACGGCAT CATCTGCGGG GAGGACACAG ACCTGGACGG CTGGCCCAAC 
GAGGACCTGC TGTGCGTGGC CAATGCAACT TACCACTGCA GAAAGGATAA TTGTCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGTGATGAT 
GACGATGACA ATGATAAGAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTATG ACTATGACAG AGATGACGTG GGAGACCGCT GTGACAACTG CCCCTACAAC 
CACAACCCAG ACCAGGCTGA CACAGATAAC AATGGGGAAG GAGACGCCTG CGCAGCTGAC 
ATTGATGGGG ACGGTATCCT CAATGAACGG GACAACTGCC AGTATGTCTA CAATGTGGAC 
CAGAAAGACA CTGACATGGA CGGGGTTGGT GACCAGTGTG ACAACTGCCC CCTGGAACAC 
AATCCAGACC AGCTCGACTC TGACTCGGAC CGCATTGGAG ACACCTGTGA CAACAATCAG 
GATATTGATG AAGACGGCCA CCAGAACAAC CTGGACAACT GTCCCTACGT GCCCAATGCC 
AACCAGGCCG ACCACGACAA GGATGGCAAA GGCGACGCCT GCGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACCG GGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC 
TCTGATGGTG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCAGGA CAAGGTGCCA 
GACATTGATG ACATCTGTCC CGAAAATGTT GATATCAGTG AGACTGATTT CCGCCGATTC 
CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CTGGACTTGC TGTAGGTTAT 
GACGAATTTA ATGCTGTGGA CTTCAGCGGG ACCTTCTTCA TCAACACCGA GAGGGATGAC 
GACTATGCCG GCTTTGTGTT TGGCTACCAG TCCAGCAGCC GCTTCTATGT TGTGATGTGG 
AAGCAAGTCA CTCAATCCTA TTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCTGGA 
CTTTCTGTGA AAGTTGTGAA CTCCACCACG GGGCCTGGCG AGCACCTGCG GAATGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACACTGT GGCATGACCC TCGTCACATT 
GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTGAGCC ACAGGCCAAA GACAGGTTTC 
ATCAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAC 
AAAACCTATG CTGGTGGTAG GCTAGGCTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCCGACCTGA AATATGAATG CAGAGATTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952418.1
CDS	197..3709
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_015096932.1
Organism	Ovis aries(sheep)
Definition	Ovis aries thrombospondin 1 (THBS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015096932.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCAGACGAT 
GGCTGGTCCC CGTGGTCTGA GTGGACCTCT TGCTCTGTGA CCTGTGGCAA TGGAATCCAG 
CAGCGTGGCC GCTCCTGCGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC TGTGCAGACG 
CGGACCTGCC ACATCCAGGA GTGTGACAAG AGATTTAAAC AGGATGGAGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCCGTAACA TGTGGAGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CCCCCAGCCC CCAGATGAAT GGGAAGCCAT GTGAGGGCAA AGCCCGGGAG 
ACCAAAGCCT GCCAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGCCC CTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGCGGAGGA GGGGCACAGA AACGTAGCCG GCTCTGCAAC 
AACCCCACGC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AACCCAGATC 
TGCAACAAGC AGGACTGTCC CATTGATGGC TGCCTGTCCA ATCCCTGCTT TGCTGGTGTC 
CAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGTGGTG CTTGTCCCCC AGGCTATAGC 
GGAGATGGCG TCAAGTGCGA AGATGTTGAT GAGTGCAAAG AAGTTCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACAG GTGTGAGAAC ACAGACCCCG GCTACAACTG CCTGCCCTGC 
CCACCACGCT TCACTGGCTC GCAGCCCTTC GGCCGGGGCG TGGAACATGC CACCGCCAAC 
AAACAGGTAT GCAAGCCCCG AAACCCCTGC ACGGACGGGA CACATGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTACAGT GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCCG GCAACGGCAT CATCTGCGGG GAGGACACAG ACCTGGACGG CTGGCCCAAC 
GAGGACCTGC TGTGCGTGGC CAATGCAACT TACCACTGCA GAAAGGATAA TTGTCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGTGATGAT 
GACGATGACA ATGATAAGAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTATG ACTATGACAG AGATGACGTG GGAGACCGCT GTGACAACTG CCCCTACAAC 
CACAACCCAG ACCAGGCTGA CACAGATAAC AATGGGGAAG GAGACGCCTG CGCAGCTGAC 
ATTGATGGGG ACGGTATCCT CAATGAACGG GACAACTGCC AGTATGTCTA CAATGTGGAC 
CAGAAAGACA CTGACATGGA CGGGGTTGGT GACCAGTGTG ACAACTGCCC CCTGGAACAC 
AATCCAGACC AGCTCGACTC TGACTCGGAC CGCATTGGAG ACACCTGTGA CAACAATCAG 
GATATTGATG AAGACGGCCA CCAGAACAAC CTGGACAACT GTCCCTACGT GCCCAATGCC 
AACCAGGCCG ACCACGACAA GGATGGCAAA GGCGACGCCT GCGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACCG GGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC 
TCTGATGGTG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCAGGA CAAGGTGCCA 
GACATTGATG ACATCTGTCC CGAAAATGTT GATATCAGTG AGACTGATTT CCGCCGATTC 
CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CTGGACTTGC TGTAGGTTAT 
GACGAATTTA ATGCTGTGGA CTTCAGCGGG ACCTTCTTCA TCAACACCGA GAGGGATGAC 
GACTATGCCG GCTTTGTGTT TGGCTACCAG TCCAGCAGCC GCTTCTATGT TGTGATGTGG 
AAGCAAGTCA CTCAATCCTA TTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCTGGA 
CTTTCTGTGA AAGTTGTGAA CTCCACCACG GGGCCTGGCG AGCACCTGCG GAATGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACACTGT GGCATGACCC TCGTCACATT 
GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTGAGCC ACAGGCCAAA GACAGGTTTC 
ATCAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAC 
AAAACCTATG CTGGTGGTAG GCTAGGCTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCCGACCTGA AATATGAATG CAGAGATTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_015096930.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952416.1 Ovis aries thrombospondin 1 (THBS1), transcript variant X2, mRNA.

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CloneID	OSm27270
Clone ID Related Accession (Same CDS sequence)	XM_015096930.1
Accession Version	XM_015096930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	thrombospondin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAACGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGTCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGACG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC CGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952416.1
CDS	197..3712
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSATSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_015096930.1
Organism	Ovis aries(sheep)
Definition	Ovis aries thrombospondin 1 (THBS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015096930.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAACGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGTCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGACG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC CGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_015096931.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952417.1 Ovis aries thrombospondin 1 (THBS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSm27271
Clone ID Related Accession (Same CDS sequence)	XM_015096931.1
Accession Version	XM_015096931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	thrombospondin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CAGCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGTCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGACG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC CGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952417.1
CDS	197..3712
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPASDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_015096931.1
Organism	Ovis aries(sheep)
Definition	Ovis aries thrombospondin 1 (THBS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015096931.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CAGCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGTCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGACG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC CGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_015096929.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014952415.1 Ovis aries thrombospondin 1 (THBS1), transcript variant X1, mRNA.

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CloneID	OSm27272
Clone ID Related Accession (Same CDS sequence)	XM_015096929.1
Accession Version	XM_015096929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	thrombospondin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639016.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAACGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCAGCCAG CGACTCTGCA 
GACGATGGCT GGTCCCCGTG GTCTGAGTGG ACCTCTTGCT CTGTGACCTG TGGCAATGGA 
ATCCAGCAGC GTGGCCGCTC CTGCGACAGC CTCAACAACA GATGCGAGGG CTCCTCTGTG 
CAGACGCGGA CCTGCCACAT CCAGGAGTGT GACAAGAGAT TTAAACAGGA TGGAGGCTGG 
AGCCACTGGT CCCCGTGGTC ATCTTGTTCC GTAACATGTG GAGATGGTGT GATCACAAGG 
ATCCGGCTCT GCAACTCCCC CAGCCCCCAG ATGAATGGGA AGCCATGTGA GGGCAAAGCC 
CGGGAGACCA AAGCCTGCCA GAAAGACGCC TGCCCCATCA ATGGAGGCTG GGGCCCCTGG 
TCACCATGGG ACATCTGTTC TGTCACCTGC GGAGGAGGGG CACAGAAACG TAGCCGGCTC 
TGCAACAACC CCACGCCCCA GTTTGGAGGC AAGGACTGCG TTGGTGATGT AACAGAAACC 
CAGATCTGCA ACAAGCAGGA CTGTCCCATT GATGGCTGCC TGTCCAATCC CTGCTTTGCT 
GGTGTCCAGT GTACCAGCTA CCCTGATGGC AGCTGGAAGT GTGGTGCTTG TCCCCCAGGC 
TATAGCGGAG ATGGCGTCAA GTGCGAAGAT GTTGATGAGT GCAAAGAAGT TCCTGATGCC 
TGCTTCAACC ACAATGGAGA GCACAGGTGT GAGAACACAG ACCCCGGCTA CAACTGCCTG 
CCCTGCCCAC CACGCTTCAC TGGCTCGCAG CCCTTCGGCC GGGGCGTGGA ACATGCCACC 
GCCAACAAAC AGGTATGCAA GCCCCGAAAC CCCTGCACGG ACGGGACACA TGACTGCAAC 
AAGAACGCCA AGTGCAACTA CCTGGGCCAC TACAGTGACC CCATGTACCG CTGCGAGTGC 
AAGCCTGGCT ACGCCGGCAA CGGCATCATC TGCGGGGAGG ACACAGACCT GGACGGCTGG 
CCCAACGAGG ACCTGCTGTG CGTGGCCAAT GCAACTTACC ACTGCAGAAA GGATAATTGT 
CCCAACCTTC CCAACTCAGG GCAGGAAGAC TATGACAAGG ATGGAATCGG CGATGCCTGT 
GATGATGACG ATGACAATGA TAAGATTCCA GATGACAGGG ACAACTGTCC ATTCCATTAC 
AACCCAGCCC AGTATGACTA TGACAGAGAT GACGTGGGAG ACCGCTGTGA CAACTGCCCC 
TACAACCACA ACCCAGACCA GGCTGACACA GATAACAATG GGGAAGGAGA CGCCTGCGCA 
GCTGACATTG ATGGGGACGG TATCCTCAAT GAACGGGACA ACTGCCAGTA TGTCTACAAT 
GTGGACCAGA AAGACACTGA CATGGACGGG GTTGGTGACC AGTGTGACAA CTGCCCCCTG 
GAACACAATC CAGACCAGCT CGACTCTGAC TCGGACCGCA TTGGAGACAC CTGTGACAAC 
AATCAGGATA TTGATGAAGA CGGCCACCAG AACAACCTGG ACAACTGTCC CTACGTGCCC 
AATGCCAACC AGGCCGACCA CGACAAGGAT GGCAAAGGCG ACGCCTGCGA CCACGATGAT 
GACAACGATG GCATTCCTGA TGACCGGGAC AACTGCAGGC TGGTGCCCAA TCCTGACCAG 
AAGGACTCTG ATGGTGATGG TCGAGGTGAT GCTTGCAAAG ATGATTTTGA CCAGGACAAG 
GTGCCAGACA TTGATGACAT CTGTCCCGAA AATGTTGATA TCAGTGAGAC TGATTTCCGC 
CGATTCCAGA TGATTCCTCT AGATCCCAAA GGGACATCCC AGAATGACCC TAACTGGGTT 
GTACGCCATC AGGGTAAAGA ACTCGTCCAG ACTGTCAACT GTGACCCTGG ACTTGCTGTA 
GGTTATGACG AATTTAATGC TGTGGACTTC AGCGGGACCT TCTTCATCAA CACCGAGAGG 
GATGACGACT ATGCCGGCTT TGTGTTTGGC TACCAGTCCA GCAGCCGCTT CTATGTTGTG 
ATGTGGAAGC AAGTCACTCA ATCCTATTGG GACACCAACC CCACGAGGGC TCAGGGATAC 
TCTGGACTTT CTGTGAAAGT TGTGAACTCC ACCACGGGGC CTGGCGAGCA CCTGCGGAAT 
GCCCTGTGGC ACACAGGAAA CACCCCTGGC CAGGTGCGCA CACTGTGGCA TGACCCTCGT 
CACATTGGCT GGAAAGATTT CACTGCCTAC AGATGGCGTC TGAGCCACAG GCCAAAGACA 
GGTTTCATCA GAGTGGTGAT GTATGAAGGG AAGAAAATCA TGGCTGACTC AGGACCCATC 
TATGACAAAA CCTATGCTGG TGGTAGGCTA GGCTTGTTCG TCTTCTCTCA AGAAATGGTG 
TTCTTCTCCG ACCTGAAATA TGAATGCAGA GATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014952415.1
CDS	197..3715
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSATSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPASDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_015096929.1
Organism	Ovis aries(sheep)
Definition	Ovis aries thrombospondin 1 (THBS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015096929.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAACGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCAGCCAG CGACTCTGCA 
GACGATGGCT GGTCCCCGTG GTCTGAGTGG ACCTCTTGCT CTGTGACCTG TGGCAATGGA 
ATCCAGCAGC GTGGCCGCTC CTGCGACAGC CTCAACAACA GATGCGAGGG CTCCTCTGTG 
CAGACGCGGA CCTGCCACAT CCAGGAGTGT GACAAGAGAT TTAAACAGGA TGGAGGCTGG 
AGCCACTGGT CCCCGTGGTC ATCTTGTTCC GTAACATGTG GAGATGGTGT GATCACAAGG 
ATCCGGCTCT GCAACTCCCC CAGCCCCCAG ATGAATGGGA AGCCATGTGA GGGCAAAGCC 
CGGGAGACCA AAGCCTGCCA GAAAGACGCC TGCCCCATCA ATGGAGGCTG GGGCCCCTGG 
TCACCATGGG ACATCTGTTC TGTCACCTGC GGAGGAGGGG CACAGAAACG TAGCCGGCTC 
TGCAACAACC CCACGCCCCA GTTTGGAGGC AAGGACTGCG TTGGTGATGT AACAGAAACC 
CAGATCTGCA ACAAGCAGGA CTGTCCCATT GATGGCTGCC TGTCCAATCC CTGCTTTGCT 
GGTGTCCAGT GTACCAGCTA CCCTGATGGC AGCTGGAAGT GTGGTGCTTG TCCCCCAGGC 
TATAGCGGAG ATGGCGTCAA GTGCGAAGAT GTTGATGAGT GCAAAGAAGT TCCTGATGCC 
TGCTTCAACC ACAATGGAGA GCACAGGTGT GAGAACACAG ACCCCGGCTA CAACTGCCTG 
CCCTGCCCAC CACGCTTCAC TGGCTCGCAG CCCTTCGGCC GGGGCGTGGA ACATGCCACC 
GCCAACAAAC AGGTATGCAA GCCCCGAAAC CCCTGCACGG ACGGGACACA TGACTGCAAC 
AAGAACGCCA AGTGCAACTA CCTGGGCCAC TACAGTGACC CCATGTACCG CTGCGAGTGC 
AAGCCTGGCT ACGCCGGCAA CGGCATCATC TGCGGGGAGG ACACAGACCT GGACGGCTGG 
CCCAACGAGG ACCTGCTGTG CGTGGCCAAT GCAACTTACC ACTGCAGAAA GGATAATTGT 
CCCAACCTTC CCAACTCAGG GCAGGAAGAC TATGACAAGG ATGGAATCGG CGATGCCTGT 
GATGATGACG ATGACAATGA TAAGATTCCA GATGACAGGG ACAACTGTCC ATTCCATTAC 
AACCCAGCCC AGTATGACTA TGACAGAGAT GACGTGGGAG ACCGCTGTGA CAACTGCCCC 
TACAACCACA ACCCAGACCA GGCTGACACA GATAACAATG GGGAAGGAGA CGCCTGCGCA 
GCTGACATTG ATGGGGACGG TATCCTCAAT GAACGGGACA ACTGCCAGTA TGTCTACAAT 
GTGGACCAGA AAGACACTGA CATGGACGGG GTTGGTGACC AGTGTGACAA CTGCCCCCTG 
GAACACAATC CAGACCAGCT CGACTCTGAC TCGGACCGCA TTGGAGACAC CTGTGACAAC 
AATCAGGATA TTGATGAAGA CGGCCACCAG AACAACCTGG ACAACTGTCC CTACGTGCCC 
AATGCCAACC AGGCCGACCA CGACAAGGAT GGCAAAGGCG ACGCCTGCGA CCACGATGAT 
GACAACGATG GCATTCCTGA TGACCGGGAC AACTGCAGGC TGGTGCCCAA TCCTGACCAG 
AAGGACTCTG ATGGTGATGG TCGAGGTGAT GCTTGCAAAG ATGATTTTGA CCAGGACAAG 
GTGCCAGACA TTGATGACAT CTGTCCCGAA AATGTTGATA TCAGTGAGAC TGATTTCCGC 
CGATTCCAGA TGATTCCTCT AGATCCCAAA GGGACATCCC AGAATGACCC TAACTGGGTT 
GTACGCCATC AGGGTAAAGA ACTCGTCCAG ACTGTCAACT GTGACCCTGG ACTTGCTGTA 
GGTTATGACG AATTTAATGC TGTGGACTTC AGCGGGACCT TCTTCATCAA CACCGAGAGG 
GATGACGACT ATGCCGGCTT TGTGTTTGGC TACCAGTCCA GCAGCCGCTT CTATGTTGTG 
ATGTGGAAGC AAGTCACTCA ATCCTATTGG GACACCAACC CCACGAGGGC TCAGGGATAC 
TCTGGACTTT CTGTGAAAGT TGTGAACTCC ACCACGGGGC CTGGCGAGCA CCTGCGGAAT 
GCCCTGTGGC ACACAGGAAA CACCCCTGGC CAGGTGCGCA CACTGTGGCA TGACCCTCGT 
CACATTGGCT GGAAAGATTT CACTGCCTAC AGATGGCGTC TGAGCCACAG GCCAAAGACA 
GGTTTCATCA GAGTGGTGAT GTATGAAGGG AAGAAAATCA TGGCTGACTC AGGACCCATC 
TATGACAAAA CCTATGCTGG TGGTAGGCTA GGCTTGTTCG TCTTCTCTCA AGAAATGGTG 
TTCTTCTCCG ACCTGAAATA TGAATGCAGA GATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_027971663.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_027827464.1 Ovis aries thrombospondin 1 (THBS1), transcript variant X1, mRNA.

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CloneID	OSm82786
Clone ID Related Accession (Same CDS sequence)	XM_027971663.1
Accession Version	XM_027971663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	thrombospondin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040258.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAATGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGATG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC CGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027827464.1
CDS	112..3627
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSATSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_027971663.1
Organism	Ovis aries(sheep)
Definition	Ovis aries thrombospondin 1 (THBS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027971663.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAATGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGATG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC CGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_027971664.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_027827465.1 Ovis aries thrombospondin 1 (THBS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSm82787
Clone ID Related Accession (Same CDS sequence)	XM_027971664.1
Accession Version	XM_027971664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	thrombospondin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040258.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAATGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CAGCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGATG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC CGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027827465.1
CDS	112..3627
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPASDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_027971664.1
Organism	Ovis aries(sheep)
Definition	Ovis aries thrombospondin 1 (THBS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027971664.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAATGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CAGCCAGCGA CTCTGCAGAC 
GATGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG AGGCTGGAGC 
CACTGGTCCC CGTGGTCATC TTGTTCCGTA ACATGTGGAG ATGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACGCCTGC CCCATCAATG GAGGCTGGGG CCCCTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGCGGA GGAGGGGCAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CGCCCCAGTT TGGAGGCAAG GACTGCGTTG GTGATGTAAC AGAAACCCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAT GGCTGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCTTGTCC CCCAGGCTAT 
AGCGGAGATG GCGTCAAGTG CGAAGATGTT GATGAGTGCA AAGAAGTTCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCAC GCTTCACTGG CTCGCAGCCC TTCGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAACAGG TATGCAAGCC CCGAAACCCC TGCACGGACG GGACACATGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGTGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AACGAGGACC TGCTGTGCGT GGCCAATGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGTGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGCGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGACCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AACCTGGACA ACTGTCCCTA CGTGCCCAAT 
GCCAACCAGG CCGACCACGA CAAGGATGGC AAAGGCGATG CCTGCGACCA CGATGATGAC 
AACGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGACGAAT TTAATGCTGT GGACTTCAGC GGGACCTTCT TCATCAACAC CGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAATC CTATTGGGAC ACCAACCCCA CGAGGGCTCA GGGATACTCT 
GGACTTTCTG TGAAAGTTGT GAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCGTCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTGATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG TAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_027971665.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_027827466.1 Ovis aries thrombospondin 1 (THBS1), transcript variant X3, mRNA.

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CloneID	OSm82788
Clone ID Related Accession (Same CDS sequence)	XM_027971665.1
Accession Version	XM_027971665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	thrombospondin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040258.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAATGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCAGCCAG CGACTCTGCA 
GACGATGGCT GGTCCCCGTG GTCTGAGTGG ACCTCTTGCT CTGTGACCTG TGGCAATGGA 
ATCCAGCAGC GTGGCCGCTC CTGCGACAGC CTCAACAACA GATGCGAGGG CTCCTCTGTG 
CAGACGCGGA CCTGCCACAT CCAGGAGTGT GACAAGAGAT TTAAACAGGA TGGAGGCTGG 
AGCCACTGGT CCCCGTGGTC ATCTTGTTCC GTAACATGTG GAGATGGTGT GATCACAAGG 
ATCCGGCTCT GCAACTCCCC CAGCCCCCAG ATGAATGGGA AGCCATGTGA GGGCAAAGCC 
CGGGAGACCA AAGCCTGCCA GAAAGACGCC TGCCCCATCA ATGGAGGCTG GGGCCCCTGG 
TCACCATGGG ACATCTGTTC TGTCACCTGC GGAGGAGGGG CACAGAAACG TAGCCGGCTC 
TGCAACAACC CCACGCCCCA GTTTGGAGGC AAGGACTGCG TTGGTGATGT AACAGAAACC 
CAGATCTGCA ACAAGCAGGA CTGTCCCATT GATGGCTGCC TGTCCAATCC CTGCTTTGCT 
GGTGTCCAGT GTACCAGCTA CCCTGATGGC AGCTGGAAGT GTGGTGCTTG TCCCCCAGGC 
TATAGCGGAG ATGGCGTCAA GTGCGAAGAT GTTGATGAGT GCAAAGAAGT TCCTGATGCC 
TGCTTCAACC ACAATGGAGA GCACAGGTGT GAGAACACAG ACCCCGGCTA CAACTGCCTG 
CCCTGCCCAC CACGCTTCAC TGGCTCGCAG CCCTTCGGCC GGGGCGTGGA ACATGCCACC 
GCCAACAAAC AGGTATGCAA GCCCCGAAAC CCCTGCACGG ACGGGACACA TGACTGCAAC 
AAGAACGCCA AGTGCAACTA CCTGGGCCAC TACAGTGACC CCATGTACCG CTGCGAGTGC 
AAGCCTGGCT ACGCCGGCAA CGGCATCATC TGCGGGGAGG ACACAGACCT GGACGGCTGG 
CCCAACGAGG ACCTGCTGTG CGTGGCCAAT GCAACTTACC ACTGCAGAAA GGATAATTGC 
CCCAACCTTC CCAACTCAGG GCAGGAAGAC TATGACAAGG ATGGAATCGG CGATGCCTGT 
GATGATGACG ATGACAATGA TAAGATTCCA GATGACAGGG ACAACTGTCC ATTCCATTAC 
AACCCAGCCC AGTATGACTA TGACAGAGAT GACGTGGGAG ACCGCTGTGA CAACTGCCCC 
TACAACCACA ACCCAGACCA GGCTGACACA GATAACAATG GGGAAGGAGA CGCCTGCGCA 
GCTGACATTG ATGGGGACGG TATCCTCAAT GAACGGGACA ACTGCCAGTA TGTCTACAAT 
GTGGACCAGA AAGACACTGA CATGGACGGG GTTGGTGACC AGTGTGACAA CTGCCCCCTG 
GAACACAATC CAGACCAGCT CGACTCTGAC TCGGACCGCA TTGGAGACAC CTGTGACAAC 
AATCAGGATA TTGATGAAGA CGGCCACCAG AACAACCTGG ACAACTGTCC CTACGTGCCC 
AATGCCAACC AGGCCGACCA CGACAAGGAT GGCAAAGGCG ATGCCTGCGA CCACGATGAT 
GACAACGATG GCATTCCTGA TGACCGGGAC AACTGCAGGC TGGTGCCCAA TCCTGACCAG 
AAGGACTCTG ATGGTGATGG TCGAGGTGAT GCTTGCAAAG ATGATTTTGA CCAGGACAAG 
GTGCCAGACA TTGATGACAT CTGTCCCGAA AATGTTGATA TCAGTGAGAC TGATTTCCGC 
CGATTCCAGA TGATTCCTCT AGATCCCAAA GGGACATCCC AGAATGACCC TAACTGGGTT 
GTACGCCATC AGGGTAAAGA ACTCGTCCAG ACTGTCAACT GTGACCCTGG ACTTGCTGTA 
GGTTATGACG AATTTAATGC TGTGGACTTC AGCGGGACCT TCTTCATCAA CACCGAGAGG 
GATGACGACT ATGCCGGCTT TGTGTTTGGC TACCAGTCCA GCAGCCGCTT CTATGTTGTG 
ATGTGGAAGC AAGTCACTCA ATCCTATTGG GACACCAACC CCACGAGGGC TCAGGGATAC 
TCTGGACTTT CTGTGAAAGT TGTGAACTCC ACCACGGGGC CTGGCGAGCA CCTGCGGAAT 
GCCCTGTGGC ACACAGGAAA CACCCCTGGC CAGGTGCGCA CACTGTGGCA TGACCCTCGT 
CACATTGGCT GGAAAGATTT CACTGCCTAC AGATGGCGTC TGAGCCACAG GCCAAAGACA 
GGTTTCATCA GAGTGGTGAT GTATGAAGGG AAGAAAATCA TGGCTGACTC AGGACCCATC 
TATGACAAAA CCTATGCTGG TGGTAGGCTA GGCTTGTTCG TCTTCTCTCA AGAAATGGTG 
TTCTTCTCCG ACCTGAAATA TGAATGCAGA GATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027827466.1
CDS	170..3688
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSATSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPASDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_027971665.1
Organism	Ovis aries(sheep)
Definition	Ovis aries thrombospondin 1 (THBS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027971665.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAGTGTCTTT 
CTCACCCTTG ACAACAATGT GGTGAATGGG TCCAGCCCTG CCATCCGCAC CGACTACATC 
GGCCACAAGA CAAAGGACCT GCAAGCCATT TGTGGCATCT CATGTGACGA GCTGTCCAGC 
ATGGTCCTAG AGCTCAGGGG TCTACGCACC ATTGTGACCA CGCTGCAGGA CAGTATCCGC 
AAAGTGACCG AAGAGAACAA AGAGCTGGCC AATGAGCTGA GGAGGCCCCC ACTCTGCTAC 
CACAACGGAG TCCAGTACAG GACTGGTGAC GAGTGGACGG TGGACAGCTG CACTGAGTGT 
CGCTGCCAGA ACTCAGTTAC CATCTGCAAA AAAGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG AGAATGCTGC CCACGGTGCT GGCCAGCCAG CGACTCTGCA 
GACGATGGCT GGTCCCCGTG GTCTGAGTGG ACCTCTTGCT CTGTGACCTG TGGCAATGGA 
ATCCAGCAGC GTGGCCGCTC CTGCGACAGC CTCAACAACA GATGCGAGGG CTCCTCTGTG 
CAGACGCGGA CCTGCCACAT CCAGGAGTGT GACAAGAGAT TTAAACAGGA TGGAGGCTGG 
AGCCACTGGT CCCCGTGGTC ATCTTGTTCC GTAACATGTG GAGATGGTGT GATCACAAGG 
ATCCGGCTCT GCAACTCCCC CAGCCCCCAG ATGAATGGGA AGCCATGTGA GGGCAAAGCC 
CGGGAGACCA AAGCCTGCCA GAAAGACGCC TGCCCCATCA ATGGAGGCTG GGGCCCCTGG 
TCACCATGGG ACATCTGTTC TGTCACCTGC GGAGGAGGGG CACAGAAACG TAGCCGGCTC 
TGCAACAACC CCACGCCCCA GTTTGGAGGC AAGGACTGCG TTGGTGATGT AACAGAAACC 
CAGATCTGCA ACAAGCAGGA CTGTCCCATT GATGGCTGCC TGTCCAATCC CTGCTTTGCT 
GGTGTCCAGT GTACCAGCTA CCCTGATGGC AGCTGGAAGT GTGGTGCTTG TCCCCCAGGC 
TATAGCGGAG ATGGCGTCAA GTGCGAAGAT GTTGATGAGT GCAAAGAAGT TCCTGATGCC 
TGCTTCAACC ACAATGGAGA GCACAGGTGT GAGAACACAG ACCCCGGCTA CAACTGCCTG 
CCCTGCCCAC CACGCTTCAC TGGCTCGCAG CCCTTCGGCC GGGGCGTGGA ACATGCCACC 
GCCAACAAAC AGGTATGCAA GCCCCGAAAC CCCTGCACGG ACGGGACACA TGACTGCAAC 
AAGAACGCCA AGTGCAACTA CCTGGGCCAC TACAGTGACC CCATGTACCG CTGCGAGTGC 
AAGCCTGGCT ACGCCGGCAA CGGCATCATC TGCGGGGAGG ACACAGACCT GGACGGCTGG 
CCCAACGAGG ACCTGCTGTG CGTGGCCAAT GCAACTTACC ACTGCAGAAA GGATAATTGC 
CCCAACCTTC CCAACTCAGG GCAGGAAGAC TATGACAAGG ATGGAATCGG CGATGCCTGT 
GATGATGACG ATGACAATGA TAAGATTCCA GATGACAGGG ACAACTGTCC ATTCCATTAC 
AACCCAGCCC AGTATGACTA TGACAGAGAT GACGTGGGAG ACCGCTGTGA CAACTGCCCC 
TACAACCACA ACCCAGACCA GGCTGACACA GATAACAATG GGGAAGGAGA CGCCTGCGCA 
GCTGACATTG ATGGGGACGG TATCCTCAAT GAACGGGACA ACTGCCAGTA TGTCTACAAT 
GTGGACCAGA AAGACACTGA CATGGACGGG GTTGGTGACC AGTGTGACAA CTGCCCCCTG 
GAACACAATC CAGACCAGCT CGACTCTGAC TCGGACCGCA TTGGAGACAC CTGTGACAAC 
AATCAGGATA TTGATGAAGA CGGCCACCAG AACAACCTGG ACAACTGTCC CTACGTGCCC 
AATGCCAACC AGGCCGACCA CGACAAGGAT GGCAAAGGCG ATGCCTGCGA CCACGATGAT 
GACAACGATG GCATTCCTGA TGACCGGGAC AACTGCAGGC TGGTGCCCAA TCCTGACCAG 
AAGGACTCTG ATGGTGATGG TCGAGGTGAT GCTTGCAAAG ATGATTTTGA CCAGGACAAG 
GTGCCAGACA TTGATGACAT CTGTCCCGAA AATGTTGATA TCAGTGAGAC TGATTTCCGC 
CGATTCCAGA TGATTCCTCT AGATCCCAAA GGGACATCCC AGAATGACCC TAACTGGGTT 
GTACGCCATC AGGGTAAAGA ACTCGTCCAG ACTGTCAACT GTGACCCTGG ACTTGCTGTA 
GGTTATGACG AATTTAATGC TGTGGACTTC AGCGGGACCT TCTTCATCAA CACCGAGAGG 
GATGACGACT ATGCCGGCTT TGTGTTTGGC TACCAGTCCA GCAGCCGCTT CTATGTTGTG 
ATGTGGAAGC AAGTCACTCA ATCCTATTGG GACACCAACC CCACGAGGGC TCAGGGATAC 
TCTGGACTTT CTGTGAAAGT TGTGAACTCC ACCACGGGGC CTGGCGAGCA CCTGCGGAAT 
GCCCTGTGGC ACACAGGAAA CACCCCTGGC CAGGTGCGCA CACTGTGGCA TGACCCTCGT 
CACATTGGCT GGAAAGATTT CACTGCCTAC AGATGGCGTC TGAGCCACAG GCCAAAGACA 
GGTTTCATCA GAGTGGTGAT GTATGAAGGG AAGAAAATCA TGGCTGACTC AGGACCCATC 
TATGACAAAA CCTATGCTGG TGGTAGGCTA GGCTTGTTCG TCTTCTCTCA AGAAATGGTG 
TTCTTCTCCG ACCTGAAATA TGAATGCAGA GATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_027971666.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_027827467.1 Ovis aries thrombospondin 1 (THBS1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSm82789
Clone ID Related Accession (Same CDS sequence)	XM_027971666.1
Accession Version	XM_027971666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	thrombospondin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040258.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAATGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCAGACGAT 
GGCTGGTCCC CGTGGTCTGA GTGGACCTCT TGCTCTGTGA CCTGTGGCAA TGGAATCCAG 
CAGCGTGGCC GCTCCTGCGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC TGTGCAGACG 
CGGACCTGCC ACATCCAGGA GTGTGACAAG AGATTTAAAC AGGATGGAGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCCGTAACA TGTGGAGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CCCCCAGCCC CCAGATGAAT GGGAAGCCAT GTGAGGGCAA AGCCCGGGAG 
ACCAAAGCCT GCCAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGCCC CTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGCGGAGGA GGGGCACAGA AACGTAGCCG GCTCTGCAAC 
AACCCCACGC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AACCCAGATC 
TGCAACAAGC AGGACTGTCC CATTGATGGC TGCCTGTCCA ATCCCTGCTT TGCTGGTGTC 
CAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGTGGTG CTTGTCCCCC AGGCTATAGC 
GGAGATGGCG TCAAGTGCGA AGATGTTGAT GAGTGCAAAG AAGTTCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACAG GTGTGAGAAC ACAGACCCCG GCTACAACTG CCTGCCCTGC 
CCACCACGCT TCACTGGCTC GCAGCCCTTC GGCCGGGGCG TGGAACATGC CACCGCCAAC 
AAACAGGTAT GCAAGCCCCG AAACCCCTGC ACGGACGGGA CACATGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTACAGT GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCCG GCAACGGCAT CATCTGCGGG GAGGACACAG ACCTGGACGG CTGGCCCAAC 
GAGGACCTGC TGTGCGTGGC CAATGCAACT TACCACTGCA GAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGTGATGAT 
GACGATGACA ATGATAAGAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTATG ACTATGACAG AGATGACGTG GGAGACCGCT GTGACAACTG CCCCTACAAC 
CACAACCCAG ACCAGGCTGA CACAGATAAC AATGGGGAAG GAGACGCCTG CGCAGCTGAC 
ATTGATGGGG ACGGTATCCT CAATGAACGG GACAACTGCC AGTATGTCTA CAATGTGGAC 
CAGAAAGACA CTGACATGGA CGGGGTTGGT GACCAGTGTG ACAACTGCCC CCTGGAACAC 
AATCCAGACC AGCTCGACTC TGACTCGGAC CGCATTGGAG ACACCTGTGA CAACAATCAG 
GATATTGATG AAGACGGCCA CCAGAACAAC CTGGACAACT GTCCCTACGT GCCCAATGCC 
AACCAGGCCG ACCACGACAA GGATGGCAAA GGCGATGCCT GCGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACCG GGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC 
TCTGATGGTG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCAGGA CAAGGTGCCA 
GACATTGATG ACATCTGTCC CGAAAATGTT GATATCAGTG AGACTGATTT CCGCCGATTC 
CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CTGGACTTGC TGTAGGTTAT 
GACGAATTTA ATGCTGTGGA CTTCAGCGGG ACCTTCTTCA TCAACACCGA GAGGGATGAC 
GACTATGCCG GCTTTGTGTT TGGCTACCAG TCCAGCAGCC GCTTCTATGT TGTGATGTGG 
AAGCAAGTCA CTCAATCCTA TTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCTGGA 
CTTTCTGTGA AAGTTGTGAA CTCCACCACG GGGCCTGGCG AGCACCTGCG GAATGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACACTGT GGCATGACCC TCGTCACATT 
GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTGAGCC ACAGGCCAAA GACAGGTTTC 
ATCAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAC 
AAAACCTATG CTGGTGGTAG GCTAGGCTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCCGACCTGA AATATGAATG CAGAGATTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027827467.1
CDS	170..3682
Translation	MGLAWGLGVLLLVHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVFLTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDACPINGGWGPWSPWDICSVTCGGGAQKRSRLCNNPTPQFGGKDCVGDVTETQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVKCEDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_027971666.1
Organism	Ovis aries(sheep)
Definition	Ovis aries thrombospondin 1 (THBS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027971666.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTCGTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGTCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCTTCCC TGAGGCAAAT GAAGAAGACC 
CGAGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAATGGCA AAGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAA GCAGCACGTG 
GTGTCGGTAG AGGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGACT CCGCATTGCC 
AAAGGAGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTCTC 
ACCCTTGACA ACAATGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATCGGC 
CACAAGACAA AGGACCTGCA AGCCATTTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTAGAGC TCAGGGGTCT ACGCACCATT GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAT GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTCC AGTACAGGAC TGGTGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGAGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCAGACGAT 
GGCTGGTCCC CGTGGTCTGA GTGGACCTCT TGCTCTGTGA CCTGTGGCAA TGGAATCCAG 
CAGCGTGGCC GCTCCTGCGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC TGTGCAGACG 
CGGACCTGCC ACATCCAGGA GTGTGACAAG AGATTTAAAC AGGATGGAGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCCGTAACA TGTGGAGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CCCCCAGCCC CCAGATGAAT GGGAAGCCAT GTGAGGGCAA AGCCCGGGAG 
ACCAAAGCCT GCCAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGCCC CTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGCGGAGGA GGGGCACAGA AACGTAGCCG GCTCTGCAAC 
AACCCCACGC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AACCCAGATC 
TGCAACAAGC AGGACTGTCC CATTGATGGC TGCCTGTCCA ATCCCTGCTT TGCTGGTGTC 
CAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGTGGTG CTTGTCCCCC AGGCTATAGC 
GGAGATGGCG TCAAGTGCGA AGATGTTGAT GAGTGCAAAG AAGTTCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACAG GTGTGAGAAC ACAGACCCCG GCTACAACTG CCTGCCCTGC 
CCACCACGCT TCACTGGCTC GCAGCCCTTC GGCCGGGGCG TGGAACATGC CACCGCCAAC 
AAACAGGTAT GCAAGCCCCG AAACCCCTGC ACGGACGGGA CACATGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTACAGT GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCCG GCAACGGCAT CATCTGCGGG GAGGACACAG ACCTGGACGG CTGGCCCAAC 
GAGGACCTGC TGTGCGTGGC CAATGCAACT TACCACTGCA GAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGTGATGAT 
GACGATGACA ATGATAAGAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTATG ACTATGACAG AGATGACGTG GGAGACCGCT GTGACAACTG CCCCTACAAC 
CACAACCCAG ACCAGGCTGA CACAGATAAC AATGGGGAAG GAGACGCCTG CGCAGCTGAC 
ATTGATGGGG ACGGTATCCT CAATGAACGG GACAACTGCC AGTATGTCTA CAATGTGGAC 
CAGAAAGACA CTGACATGGA CGGGGTTGGT GACCAGTGTG ACAACTGCCC CCTGGAACAC 
AATCCAGACC AGCTCGACTC TGACTCGGAC CGCATTGGAG ACACCTGTGA CAACAATCAG 
GATATTGATG AAGACGGCCA CCAGAACAAC CTGGACAACT GTCCCTACGT GCCCAATGCC 
AACCAGGCCG ACCACGACAA GGATGGCAAA GGCGATGCCT GCGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACCG GGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC 
TCTGATGGTG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCAGGA CAAGGTGCCA 
GACATTGATG ACATCTGTCC CGAAAATGTT GATATCAGTG AGACTGATTT CCGCCGATTC 
CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CTGGACTTGC TGTAGGTTAT 
GACGAATTTA ATGCTGTGGA CTTCAGCGGG ACCTTCTTCA TCAACACCGA GAGGGATGAC 
GACTATGCCG GCTTTGTGTT TGGCTACCAG TCCAGCAGCC GCTTCTATGT TGTGATGTGG 
AAGCAAGTCA CTCAATCCTA TTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCTGGA 
CTTTCTGTGA AAGTTGTGAA CTCCACCACG GGGCCTGGCG AGCACCTGCG GAATGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACACTGT GGCATGACCC TCGTCACATT 
GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTGAGCC ACAGGCCAAA GACAGGTTTC 
ATCAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAC 
AAAACCTATG CTGGTGGTAG GCTAGGCTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCCGACCTGA AATATGAATG CAGAGATTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	THBS1
Entrez Gene ID	101106433
Full Name	thrombospondin 1
Synonyms	TSP-1
Gene Type	protein-coding
Organism	Ovis aries musimon(Ovis orientalis musimon)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

THBS1 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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