PIEZO2 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIEZO2
Entrez Gene ID	101105203
Full Name	piezo type mechanosensitive ion channel component 2
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012118687.2	XP_011974077.1	LOW QUALITY PROTEIN: piezo-type mechanosensitive ion channel component 2 isoform X3
XM_012103589.2	XP_011958979.1	piezo-type mechanosensitive ion channel component 2 isoform X2
XM_027960843.1	XP_027816644.1	piezo-type mechanosensitive ion channel component 2
XM_012103587.2	XP_011958977.1	piezo-type mechanosensitive ion channel component 2 isoform X1

Pathways from BioSystems
Gene Ontology

The following PIEZO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIEZO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv06436	XM_012118687.2 Latest version!	Ovis aries musimon piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSm26167	XM_012103587.2 Latest version!	Ovis aries piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSm26168	XM_012103589.2 Latest version!	Ovis aries piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSm73969	XM_027960843.1 Latest version!	Ovis aries piezo type mechanosensitive ion channel component 2 (PIEZO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIEZO2 ( XM_012118687.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011974077.1 Ovis aries musimon piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOv06436
Clone ID Related Accession (Same CDS sequence)	XM_012118687.2
Accession Version	XM_012118687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	LOW QUALITY PROTEIN: piezo-type mechanosensitive ion channel component 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942273.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012118687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGGCTTGG CACCCCAGTC CTGGAGACCC TTTGCTCGGA CTCGCGGGAC CGACGCGATG 
GCCTCGGAAG TGGTGTGCGG GCTCGTCTTC AGGCTGCTGC TCCCCATCTG CCTGGCAGTA 
GCCTGTGCCT TCCGGTACAA CGGGCTCTCC TTCGTCTACC TCATCTACCT CCTGCTCATC 
CCCCTGTTCT CGGAACCAAC TAAAGCCACC ATGCAAGGAC ACACGGGACG GTTGTTAAAG 
TCTCTGTGCT TCACCAGTCT CTCCTTCCTG TTGCTGCACA TCATTTTCCA CATCACGTTG 
GCGAGTCTGG AGGCACAGCA CCGCATTGCA CCTGGTTACA ACTGCTCAAC CTGGGAGAAG 
ACTTTCCGAC AAATCGGCTT CGAAAGCTTA AAGGGAGCCG ACGCTGGCAA TGGGATCCGA 
GTGTTTGTGC CTGACGTGGG CATGTTCATC GCCAGCCTGA CCATCTGGCT GGTCTGTAGG 
AACATTGTTC AGAAGCCAGT GGCCGAAGAG GCCGCGCAGT GTAACCCGGA GTTTGAGAAC 
GAAGAGTCGG CTGGAGGAGA AAAAATAGAC TCCGAAGAGG CCCTGATCTA TGAAGAGGAC 
TTAGATGAAG GAGACGGTGC TGAAGGCGAT TTGGAGGAAA GCACGAAATT AAAACTCTTC 
CGCAGGCTGG CCTCCGTGGC CTCCAAGCTG AAGGAGTTTA TTGGCAACAT GATAACTACA 
GCGGGGAAGG TTGTTGTGAC AATTTTACTG GGTTCATCAG GGATGATGCT GCCCTCTCTG 
ACCTCATCCG TGTACTTCTT CGTGTTCCTG GGCCTGTGTA CCTGGTGGTC CTGGTGCCGG 
ACCTTCGACC CCTTGCTGTT CAGCTGTCTC TGCGTCCTGC TGGCCATCTT CACTGCGGGA 
CACCTGATCG GACTCTATCT GTACCAGTTC CAGTTCTTCC AGGAAGCCGT TCCCCCCAAC 
GACTACTATG CCAGGTTGTT TGGTATCAAA TCAGTCATTC AGACGGACTG TTCCAGCACA 
TGGAAGATCA TTGCCAACCC CGACCTGTCC TGGTACCACC ACGCCAACCC CATCGTCCTG 
CTGGTGATGT ACTACACGCT GGCCACTCTG ATCCGAATCT GGCTGCAGGA GCCCGTTGTG 
CAGGAAGAGA AGACCAAGGA GGAGGACAGA GCCCTGGCCT GCAGCCCCGC GCCCATCACG 
GCCGAGAGGA GGCGGAGCCT GTGGTACGCG ACCCACTACC CCACAGACGA GAGAAAACTT 
TTATCCATGA CTCAGGATGA CTACAAGCCT TCTGACGGCC TGCTGGTGAC CGTGAATGGC 
AACCCCGTGG ACTACCACAC CATCCACCCC AGCCTCCCAC TGGAGAACGG CCCCGCCAAG 
GCTGACCTGT ACTCCACACC ACAGTACCGC TGGGAGCCCG CCACTGCCTC CCCTGAAAAG 
AAGGAGGAAG ATAATGATGA GAAAGAAGAG TTTGAGGAGG AGCGGAGCCG GGAGGAGAAG 
AGGAGTGTCA AAGTGCACGC CATGGTGTCT GTGTTCCAGT TTATCATGAA ACAGAGCTAC 
ATCTGTGCCC TCATCGCCAT GATGGCCTGG AGCATCACCT ACCACAGCTG GCTGACCTTC 
GTGCTGCTCA TCTGGTCCTG CACCCTGTGG ATGATCCGCA ACCGGAGGAA GTATGCCATG 
ATCAGCTCCC CCTTCATGGT CGTTTACGGA AACCTGCTGC TCATACTGCA GTACATATGG 
AGCTTCGAGC TCCCTGAGAT TAAAAAGGTC CCGGGTTTCT TAGAAAAGAA GGAGCCGGGG 
GAGCTCGCCT CAAAGATTCT GTTCACCATC ACATTCTGGC TGCTGCTGAG ACAGCACCTC 
ACAGAGCAGA AAGCTCTCCA AGAAAAGGAA GCTCTTCTGT CTGAGGTCAA AATCGGAAGT 
CAGGAAAATG AAGAAAAAGA TGATGAACAG GATGTACAGG TAGACAGCGA GGCTCAGGAG 
AAGGAGGAAG GAGAGGAGCC GAAGGAGGAG CAGCAGGAGC GGAGAGAGGA AGAGGAGGAG 
GAAGAGGCAG ACGAGCAGGA CGTGATGAAG GTGCTGGGGA ACCTGGTGGT GGCCCTGTTC 
ATCAAGTACT GGATCTACCT CTGCGGGGGG ATGTTCTTCT TCGTCAGCTT CGAGGGTAAA 
ATCGTGATGT ACAAGATCAT CTACATGGTA CTCTTCCTCT TCTGTGTGGC CCTGTATCAG 
GTGCACTATG AATGGTGGAG GAGGATTCTG AAGTACTTCT GGATGTCGGT GGTTATCTAC 
ACAATGCTGG TGCTTATCTT TATATACACA TATCAGTTTG AAAACTTCCC AGGCTTGTGG 
CAAAACATGA CTGGACTGAA AAAAGAAAAG CTGGAGGACC TTGGCCTGAA GCAGTTCACG 
GTGGCCGAGC TGTTCACGCG CATCTTCATC CCCACCTCCT TCCTCCTGGT CTGCATTCTG 
CACCTGCACT ACTTCCACGA CCGATTCCTG GAGCTCACCG ACCTGAAGTC CATCCCCAGC 
AAGGAGGACA GCGCTGTCTA TAGGCTGGCC CACCCCGAGG GAAGCCTTCC AGACCTTGCC 
ATGATGAAAC TGACTGCCAG CCTGGAGGTG GCAGAGGTCA AGCAGCTGGC TGGGCCTGGG 
GAGGAGATGC CAGGGGGATG CCCTGCAAAA GCCAAGAAGG GCGAGCCTGG CAGGGACAGC 
GAGGATGAGG ATGAGGATGA GGACGAGGAG GATGAAGAGG AGGAGGAGAC ATCAGATCTG 
AGGAACAAGT GGCACCTGGT GATCGACCGC CTCACGGTGC TGCTGCTGCG ATTCCTGGAG 
GGCTTCCACC AGCTGCAGAC ATTCCTGTGG TGGATCCTGG AGCTGCACAT CGTCAAGATT 
GTGTCCTCCT ACATCATCTG GGTGTCCGTG AAGGAGGTGT CTCTGTTCAA CTACGTGTTT 
CTGATTTCCT GGGCTTTTGC TCTGCCGTAT GCTAAGCTCC GCCGTCTGGC CTCCAGTGTC 
TGCACCGTCT GGACATGTGT GATCATTGTC TGTAAGATGC TGTACCAGCT GCAGACCATC 
AAGCCTGAGA ACTTCTCTGT GAACTGTTCG TTGCCAAATG AAAACCAGAC GAACATCCCC 
CTGCACCAGC TGAACAAGTC TCTGCTCTAC AGCGCACCCA TTGACCCCAC AGAATGGGTG 
GGCCTCAGGA AGTCGTCTCC TCTACTTGTC TACCTAAGGA ACAACCTGCT GATGCTGGCC 
ATCCTGGCCT TCGAGGTCAC CATCTACCGC CATCAGGAGT ACTACCGTGG TCGGAACAAC 
CTCTCAACCC CCGTGTCTAA AACCATCTTC CACAACATCA CAAGACTCCA GCTGGATGAT 
GGACTTGTTA ACTGTGCCAA GTATTTCATC AATTACTTCT TCTACAAGTT TGGTCTGGAG 
ACCTGTTTCC TCATGTCAGT TAATGTGATT GGCCAGCGCA TGGACTTCTA CGCCATGATT 
CACGCCTGCT GGCTGATTGC CGTCCTGTAC CGGCGCAGGA GGAAGGCCAT CGCCGAGATC 
TGGCCCAAGT ACTGCTGCTT CCTGGCTTGC ATCATCACCT TCCAGTATTT CATCTGCATC 
GGCATCCCCC CTGCCCCCTG TCGAGACTAC CCTTGGAGGT TCAAGGGCGC CAGCTTCAAT 
GACAACATCG TCAAGTGGCT GTACTTCCCT GACTTCATCG TTCGACCCAA CCCTGTGTTC 
CTCGTGTATG ATTTCATGCT ACTCCTGTGT GCCTCGCTGC AACGGCAGAT CTTTGAGGAT 
GAGAATAAGG CTGCTGTACG GATCGTGGCG GGCGACAACG TGGAAATCTG CATGAACCTG 
GATGCGGCCT CATTTAGCCA GCACAATCCC GTGCCCGACT TCATCCACTG CAGGTCGTAC 
CTGGACATGT CCAAGGTGGT CATCTTCAGC TACCTCTTCT GGTTCGTGCT CACTATCATC 
TTCATCACCG GGACGACTCG CATCAGCATC TTTTGCATGG GCTACCTGGT GGCCTGCTTC 
TACTTCCTGC TCTTCGGGGG CGACTTGCTG CTGAAACCCA TCAAGAGCAT CCTGCGCTAC 
TGGGACTGGC TGATCGCTTA CAATGTCTTC GTGATCACGA TGAAAAACAT ACTGTCGATA 
GGAGCGTGTG GCTATATTGA AAAATTGGTT CAAAATAGTT GCTGGTTGAT CCAAGCATTC 
AGCCTGGCCT GTACAGTCAA GGGCTACAAA ATGCCTGATG ATGACTCCTC CTGTAAACTG 
CCCAGTGGGG AGGCAGGAAT CATTTGGGAC AGCATATGCT TTGCCTTCCT GCTGCTGCAG 
AGAAGAGTGT TCATGAGTTA CTATTTCCTG CATGTGGTGG CAGATATAAA AGCCTCACAG 
ATCCTGGCAT CCAGAGGGGC CGAGCTTTTC CAGGCCACCA TTGTGAAGGC TGTGAAGGCC 
AGAATCGAGG AGGAGAAAAA GTCCATGGAC CAGCTGAAGA GGCAGATGGA CCGCATCAAG 
GCCAGGCAAC AGAAATACAA ATAGGGTAAG GAGAGGATGC TGAGCTTGAC CCAGGAGTCG 
GGGGAAGGCC AAGATCTCCA AAAACTTTCT GAAGAGGATG ACGAGAGAGA AGCAGACAAA 
CAGAAAACCA AGGGCAAAAA AAAGCAGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG 
GTCAGGAGTG GAGATTACTA TTTGTTTGAA ACGGATAGTG AAGAGGAGGA AGAGGAAGAA 
TTAAAAAAGG AAGACGAAGA GCCCCCCCGG AGGTCAGCCT TCCAGTTTGT TTATCAAGCC 
TGGATTACTG ACCCTAAAAC AGCACTCCGA CAGAGGCGCA AAGAGAAAAA AAGATCTGCA 
AGAGAAGAAC AGAAAAGAAG GAGAAAAGAA TCTGGGGAGG GTCCTGTGGA GTGGGAAGAC 
CGGGAAGATG AGCCAGTCAA AAAGAAGTCT GATGGACCAG ACAACATCAT CAAGAGGATC 
TTTAATATTT TGAAGTTCAC CTGGGTCCTG TTTCTGGCAA CCGTGGACAG TTTCACAACG 
TGGCTCAACT CCATCTCCAG AGAGCACATC GACATCTCCA CTGTCCTGAG GATCGAGCGC 
TGCATGCTGA CCAGAGAGAT CAAGAAGGGC AACGTCCCAA CCCGGGAGAG CATCCACATG 
TACTACCAGA ACCATATCAT GAACCTGTCC AGGGAGTCGG GGCTGGACAC CATCGACGAC 
CATGCTGGCG CTGCTGCGGG CGCACAGACC GCCCACAGGA TGGACAGCTT GGATTCCCAC 
GACAGCATCT CCAGCGAGCC CACGCAGTGC ACGATGCTGT ACTCGCGCCA GGGGACCACG 
GAGACCATCG AGGAAGTGGA GGCTGAGCAG GACGAGGAGA CATCCGAGGG GGCCCGGCAG 
GCCGAGTCCC CACAGGACCC CACGGAGGAT GGTGACGTGG AGGCCCCGCC GTCCTACAGC 
AAGGCTGTCA GCTTTGAACG CCTGTCCTTC GACTCCCGGG ATGACTCAGC TGGCCAGACC 
CACATGGAAG TCAGCCCGGA TGACAGCCGC TCAGATCAGC TGGAGGCCAG CATCCTGCCA 
CCCCTGACCC ACGAGCTGAC AGCCAGCGAG CTGCTGCTGA ACAAGATGTT CCATGACGAC 
GAGCTGGAGG AGTCAGAGAA GTTCTACGTG GGGCAGCCTC GCTTCCTGCT GCTCTTCTAC 
GCGCTGTACA ACACGCTGGT GGCCCGCTCC GAGATGGTGT GCTACTTTGT GATCATCCTC 
AACCACATGG TCTCCGCCTC CATGATCACC CTCGTGCTGC CCATCCTCAT CTTCCTCTGG 
GCCATGCTGT CCGTCCCCCG GCCCAGCAGG CGCTTCTGGA TGATGGCCAT CGTCTACACG 
GAGGTGGCCA TTGTGGTCAA GTACTTCTTC CAATTTGGGT TCTTCCCCTG GAACAAGAAT 
GTGGAACTGA ACAAGGACAA GCCTTACCAC CCACCCAACA TCATCGGGGT GGAGAAGAAG 
GACGCCTACG TGCTCTACGA CCTCATCCAG CTGCTTGCAC TCTTCTTCCA CCGCTCCATC 
CTAAAGTGTC ACGGCCTCTG GGATGAAGAC GACATCGTGG ACAGCGGCTC GGACAAGGGG 
GACTCGGACG ACGAGCTGTC CCTCGGCCAT GGTAGGAGGG CCTCCTCCGA CTCGCTCAAG 
TCCATCAACC TGGCTGCATC CGTGGAGTCG GTACATGTGA CCTTCCCAGA GCAGCCGGCT 
GCCGTCCGGA GGAAGCGCTC CAGCAGCGGC TCCCAGACAT CACAGGAGTC CAGCTTTTCC 
TCGAATAGGT CCAAGAGAGG CAGCACGAGC ACCCGGAACA GCAGCCAGAA AGGAAGCAGC 
GTCGTGAGCG TCAAGCAGAA GAGCAGACGG GAACTTTACA TGGAGAAGCT GCAGGAACAC 
TTAATCAAAG CAAAGGCCTT TACCATAAAG AAGACCCTGC AGCTCTACGT GCCCATCAGG 
CAGTTCTTCT ACGACCTCAT CCACCCCGAC TACAGCGCCG TCACCGACGT GTACGTGCTT 
ATGTTCCTGG CGGACACTGT GGACTTCGTC ATCATTGTCT TCGGCTTCTG GGCCTTCGGG 
AAACACTCGG CTGCAGCCGA CATCACGTCC TCACTGTCCG AGGACCAGGT GCCAGGGCCC 
TTCCTGGTGA TGGTGCTCAT TCAGTTCGGG ACTATGGTGG TGGACCGGGC GCTCTACCTC 
AGGAAGACTG TCCTGGGAAA GGTCATCTTC CAGGTCATCC TGGTGTTTGG GATTCACTTC 
TGGATGTTCT TCATCCTGCC TGGCGTGACC GAGAGGAAGT TCAGCCAGAA CCTGGTCGCG 
CAGCTGTGGT ACTTCGTCAA GTGCGTGTAC TTCGGGCTGT CCGCCTACCA GATCCGCTGC 
GGCTACCCAA CTCGGGTCCT CGGCAACTTC CTCACCAAGA GTTACAACTA CGTCAACCTC 
TTCCTGTTCC AGGGGTTCCG CCTTGTCCCT TTCCTGACGG AGCTGAGGGC TGTGATGGAC 
TGGGTGTGGA CAGACACGAC CCTGAGCCTG TCCAGCTGGA TCTGCGTGGA GGACATCTAT 
GCTCACATCT TCATCCTCAA GTGTTGGCGG GAGTCGGAAA AAAGGTACCC GCAGCCCCGG 
GGGCAGAAGA AGAAGAAGGT GGTCAAGTAC GGGATGGGGG GCATGATCAT CGTGCTGCTC 
ATCTGCATCG TCTGGTTCCC GCTACTCTTC ATGTCTCTGG TCAAATCCGT CGCTGGGGTT 
ATCAACCAAC CCCTGGACGT CTCAGTCACA ATCACCCTGG GCGGGTACCA GCCTATTTTC 
ACAATGAGTG CCCAGCAAAG CCAGCTGAAA GTTATGGACC AGCCAATGTT TAATAAATTC 
ATTAAAGCTT TTTCTAAGGA CACTGGTGCT ATGCAATTTC TGGAAAATTA TGAAAAAGAA 
GACATAACAG TCGCAGAACT GGAAGGAAAC TCAAATTCTT TATGGACCAT CAGCCCTCCC 
AGTAAGCAGA AAATGATACA CGAACTCAAG GACCCCAATA GTAGCTTCTC TGTTGTTTTT 
TCATGGAGTA TTCAGAGAAA CATGAGTCTG GGTGCAAAAG CAGAAATAGC AACAGATAAG 
CTTTCTTTCC CTCTTAAAAA CAAGACACGA GAGAATATTG CTAAAATGAT AGCTGGCGGC 
GACACGGAAA GTTCCAGGAC GCCCGTGACC ATAGAAAGGA TTTACCCGTA CTATGTGAAA 
GCACCCAGTG ACTCTAACTC CAAACCCATA AAGCAGCTTT TATCTGAAAA TAATTTCATG 
AACATCACCA TCATTTTGTC CAGAGACAAT GCGACTAACT CGAACAGTGA GTGGTGGGTT 
CTCAACTTGA CTGGGAACAG AATCTACAAC CAGGAAGCGC AGGCCCTGGA GCTGGTGGTC 
TTCAACGACA AGGTCAGCCC CCCCAGCCTG GGGTTCCTGG CCGGCTACGG TATCATGGGC 
CTCTACGCTT CCGTGGTGCT GGTGATTGGG AAGTTTGTCC GGGAATTCTT CAGCGGGATT 
TCTCACTCCA TCATGTTTGA AGAACTTCCC AATGTGGATC GGATTCTGAA ATTGTGCACA 
GACATTTTTT TAGTCCGGGA GACCGGAGAG CTGGAGCTCG AGGAGGATCT CTATGCCAAA 
TTAATATTCC TGTACCGGTC GCCAGAAACC ATGATCAAAT GGACCAGGGA GAAAACAAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011974077.1
CDS	747..8969
Translation	MGLAPQSWRPFARTRGTDAMASEVVCGLVFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKATMQGHTGRLLKSLCFTSLSFLLLHIIFHITLASLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDVGMFIASLTIWLVCRNIVQKPVAEEAAQCNPEFENEESAGGEKIDSEEALIYEEDLDEGDGAEGDLEESTKLKLFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIANPDLSWYHHANPIVLLVMYYTLATLIRIWLQEPVVQEEKTKEEDRALACSPAPITAERRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPLENGPAKADLYSTPQYRWEPATASPEKKEEDNDEKEEFEEERSREEKRSVKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDDEQDVQVDSEAQEKEEGEEPKEEQQERREEEEEEEADEQDVMKVLGNLVVALFIKYWIYLCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRRILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDSAVYRLAHPEGSLPDLAMMKLTASLEVAEVKQLAGPGEEMPGGCPAKAKKGEPGRDSEDEDEDEDEEDEEEEETSDLRNKWHLVIDRLTVLLLRFLEGFHQLQTFLWWILELHIVKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPLHQLNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLSTPVSKTIFHNITRLQLDDGLVNCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIVKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIVAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVVIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIEKLVQNSCWLIQAFSLACTVKGYKMPDDDSSCKLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYK*GKERMLSLTQESGEGQDLQKLSEEDDEREADKQKTKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRRKEKKRSAREEQKRRRKESGEGPVEWEDREDEPVKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDDHAGAAAGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQDEETSEGARQAESPQDPTEDGDVEAPPSYSKAVSFERLSFDSRDDSAGQTHMEVSPDDSRSDQLEASILPPLTHELTASELLLNKMFHDDELEESEKFYVGQPRFLLLFYALYNTLVARSEMVCYFVIILNHMVSASMITLVLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVELNKDKPYHPPNIIGVEKKDAYVLYDLIQLLALFFHRSILKCHGLWDEDDIVDSGSDKGDSDDELSLGHGRRASSDSLKSINLAASVESVHVTFPEQPAAVRRKRSSSGSQTSQESSFSSNRSKRGSTSTRNSSQKGSSVVSVKQKSRRELYMEKLQEHLIKAKAFTIKKTLQLYVPIRQFFYDLIHPDYSAVTDVYVLMFLADTVDFVIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLVKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQPMFNKFIKAFSKDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELKDPNSSFSVVFSWSIQRNMSLGAKAEIATDKLSFPLKNKTRENIAKMIAGGDTESSRTPVTIERIYPYYVKAPSDSNSKPIKQLLSENNFMNITIILSRDNATNSNSEWWVLNLTGNRIYNQEAQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_012118687.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012118687.2

ORF Insert Sequence:

ATGGGCTTGG CACCCCAGTC CTGGAGACCC TTTGCTCGGA CTCGCGGGAC CGACGCGATG 
GCCTCGGAAG TGGTGTGCGG GCTCGTCTTC AGGCTGCTGC TCCCCATCTG CCTGGCAGTA 
GCCTGTGCCT TCCGGTACAA CGGGCTCTCC TTCGTCTACC TCATCTACCT CCTGCTCATC 
CCCCTGTTCT CGGAACCAAC TAAAGCCACC ATGCAAGGAC ACACGGGACG GTTGTTAAAG 
TCTCTGTGCT TCACCAGTCT CTCCTTCCTG TTGCTGCACA TCATTTTCCA CATCACGTTG 
GCGAGTCTGG AGGCACAGCA CCGCATTGCA CCTGGTTACA ACTGCTCAAC CTGGGAGAAG 
ACTTTCCGAC AAATCGGCTT CGAAAGCTTA AAGGGAGCCG ACGCTGGCAA TGGGATCCGA 
GTGTTTGTGC CTGACGTGGG CATGTTCATC GCCAGCCTGA CCATCTGGCT GGTCTGTAGG 
AACATTGTTC AGAAGCCAGT GGCCGAAGAG GCCGCGCAGT GTAACCCGGA GTTTGAGAAC 
GAAGAGTCGG CTGGAGGAGA AAAAATAGAC TCCGAAGAGG CCCTGATCTA TGAAGAGGAC 
TTAGATGAAG GAGACGGTGC TGAAGGCGAT TTGGAGGAAA GCACGAAATT AAAACTCTTC 
CGCAGGCTGG CCTCCGTGGC CTCCAAGCTG AAGGAGTTTA TTGGCAACAT GATAACTACA 
GCGGGGAAGG TTGTTGTGAC AATTTTACTG GGTTCATCAG GGATGATGCT GCCCTCTCTG 
ACCTCATCCG TGTACTTCTT CGTGTTCCTG GGCCTGTGTA CCTGGTGGTC CTGGTGCCGG 
ACCTTCGACC CCTTGCTGTT CAGCTGTCTC TGCGTCCTGC TGGCCATCTT CACTGCGGGA 
CACCTGATCG GACTCTATCT GTACCAGTTC CAGTTCTTCC AGGAAGCCGT TCCCCCCAAC 
GACTACTATG CCAGGTTGTT TGGTATCAAA TCAGTCATTC AGACGGACTG TTCCAGCACA 
TGGAAGATCA TTGCCAACCC CGACCTGTCC TGGTACCACC ACGCCAACCC CATCGTCCTG 
CTGGTGATGT ACTACACGCT GGCCACTCTG ATCCGAATCT GGCTGCAGGA GCCCGTTGTG 
CAGGAAGAGA AGACCAAGGA GGAGGACAGA GCCCTGGCCT GCAGCCCCGC GCCCATCACG 
GCCGAGAGGA GGCGGAGCCT GTGGTACGCG ACCCACTACC CCACAGACGA GAGAAAACTT 
TTATCCATGA CTCAGGATGA CTACAAGCCT TCTGACGGCC TGCTGGTGAC CGTGAATGGC 
AACCCCGTGG ACTACCACAC CATCCACCCC AGCCTCCCAC TGGAGAACGG CCCCGCCAAG 
GCTGACCTGT ACTCCACACC ACAGTACCGC TGGGAGCCCG CCACTGCCTC CCCTGAAAAG 
AAGGAGGAAG ATAATGATGA GAAAGAAGAG TTTGAGGAGG AGCGGAGCCG GGAGGAGAAG 
AGGAGTGTCA AAGTGCACGC CATGGTGTCT GTGTTCCAGT TTATCATGAA ACAGAGCTAC 
ATCTGTGCCC TCATCGCCAT GATGGCCTGG AGCATCACCT ACCACAGCTG GCTGACCTTC 
GTGCTGCTCA TCTGGTCCTG CACCCTGTGG ATGATCCGCA ACCGGAGGAA GTATGCCATG 
ATCAGCTCCC CCTTCATGGT CGTTTACGGA AACCTGCTGC TCATACTGCA GTACATATGG 
AGCTTCGAGC TCCCTGAGAT TAAAAAGGTC CCGGGTTTCT TAGAAAAGAA GGAGCCGGGG 
GAGCTCGCCT CAAAGATTCT GTTCACCATC ACATTCTGGC TGCTGCTGAG ACAGCACCTC 
ACAGAGCAGA AAGCTCTCCA AGAAAAGGAA GCTCTTCTGT CTGAGGTCAA AATCGGAAGT 
CAGGAAAATG AAGAAAAAGA TGATGAACAG GATGTACAGG TAGACAGCGA GGCTCAGGAG 
AAGGAGGAAG GAGAGGAGCC GAAGGAGGAG CAGCAGGAGC GGAGAGAGGA AGAGGAGGAG 
GAAGAGGCAG ACGAGCAGGA CGTGATGAAG GTGCTGGGGA ACCTGGTGGT GGCCCTGTTC 
ATCAAGTACT GGATCTACCT CTGCGGGGGG ATGTTCTTCT TCGTCAGCTT CGAGGGTAAA 
ATCGTGATGT ACAAGATCAT CTACATGGTA CTCTTCCTCT TCTGTGTGGC CCTGTATCAG 
GTGCACTATG AATGGTGGAG GAGGATTCTG AAGTACTTCT GGATGTCGGT GGTTATCTAC 
ACAATGCTGG TGCTTATCTT TATATACACA TATCAGTTTG AAAACTTCCC AGGCTTGTGG 
CAAAACATGA CTGGACTGAA AAAAGAAAAG CTGGAGGACC TTGGCCTGAA GCAGTTCACG 
GTGGCCGAGC TGTTCACGCG CATCTTCATC CCCACCTCCT TCCTCCTGGT CTGCATTCTG 
CACCTGCACT ACTTCCACGA CCGATTCCTG GAGCTCACCG ACCTGAAGTC CATCCCCAGC 
AAGGAGGACA GCGCTGTCTA TAGGCTGGCC CACCCCGAGG GAAGCCTTCC AGACCTTGCC 
ATGATGAAAC TGACTGCCAG CCTGGAGGTG GCAGAGGTCA AGCAGCTGGC TGGGCCTGGG 
GAGGAGATGC CAGGGGGATG CCCTGCAAAA GCCAAGAAGG GCGAGCCTGG CAGGGACAGC 
GAGGATGAGG ATGAGGATGA GGACGAGGAG GATGAAGAGG AGGAGGAGAC ATCAGATCTG 
AGGAACAAGT GGCACCTGGT GATCGACCGC CTCACGGTGC TGCTGCTGCG ATTCCTGGAG 
GGCTTCCACC AGCTGCAGAC ATTCCTGTGG TGGATCCTGG AGCTGCACAT CGTCAAGATT 
GTGTCCTCCT ACATCATCTG GGTGTCCGTG AAGGAGGTGT CTCTGTTCAA CTACGTGTTT 
CTGATTTCCT GGGCTTTTGC TCTGCCGTAT GCTAAGCTCC GCCGTCTGGC CTCCAGTGTC 
TGCACCGTCT GGACATGTGT GATCATTGTC TGTAAGATGC TGTACCAGCT GCAGACCATC 
AAGCCTGAGA ACTTCTCTGT GAACTGTTCG TTGCCAAATG AAAACCAGAC GAACATCCCC 
CTGCACCAGC TGAACAAGTC TCTGCTCTAC AGCGCACCCA TTGACCCCAC AGAATGGGTG 
GGCCTCAGGA AGTCGTCTCC TCTACTTGTC TACCTAAGGA ACAACCTGCT GATGCTGGCC 
ATCCTGGCCT TCGAGGTCAC CATCTACCGC CATCAGGAGT ACTACCGTGG TCGGAACAAC 
CTCTCAACCC CCGTGTCTAA AACCATCTTC CACAACATCA CAAGACTCCA GCTGGATGAT 
GGACTTGTTA ACTGTGCCAA GTATTTCATC AATTACTTCT TCTACAAGTT TGGTCTGGAG 
ACCTGTTTCC TCATGTCAGT TAATGTGATT GGCCAGCGCA TGGACTTCTA CGCCATGATT 
CACGCCTGCT GGCTGATTGC CGTCCTGTAC CGGCGCAGGA GGAAGGCCAT CGCCGAGATC 
TGGCCCAAGT ACTGCTGCTT CCTGGCTTGC ATCATCACCT TCCAGTATTT CATCTGCATC 
GGCATCCCCC CTGCCCCCTG TCGAGACTAC CCTTGGAGGT TCAAGGGCGC CAGCTTCAAT 
GACAACATCG TCAAGTGGCT GTACTTCCCT GACTTCATCG TTCGACCCAA CCCTGTGTTC 
CTCGTGTATG ATTTCATGCT ACTCCTGTGT GCCTCGCTGC AACGGCAGAT CTTTGAGGAT 
GAGAATAAGG CTGCTGTACG GATCGTGGCG GGCGACAACG TGGAAATCTG CATGAACCTG 
GATGCGGCCT CATTTAGCCA GCACAATCCC GTGCCCGACT TCATCCACTG CAGGTCGTAC 
CTGGACATGT CCAAGGTGGT CATCTTCAGC TACCTCTTCT GGTTCGTGCT CACTATCATC 
TTCATCACCG GGACGACTCG CATCAGCATC TTTTGCATGG GCTACCTGGT GGCCTGCTTC 
TACTTCCTGC TCTTCGGGGG CGACTTGCTG CTGAAACCCA TCAAGAGCAT CCTGCGCTAC 
TGGGACTGGC TGATCGCTTA CAATGTCTTC GTGATCACGA TGAAAAACAT ACTGTCGATA 
GGAGCGTGTG GCTATATTGA AAAATTGGTT CAAAATAGTT GCTGGTTGAT CCAAGCATTC 
AGCCTGGCCT GTACAGTCAA GGGCTACAAA ATGCCTGATG ATGACTCCTC CTGTAAACTG 
CCCAGTGGGG AGGCAGGAAT CATTTGGGAC AGCATATGCT TTGCCTTCCT GCTGCTGCAG 
AGAAGAGTGT TCATGAGTTA CTATTTCCTG CATGTGGTGG CAGATATAAA AGCCTCACAG 
ATCCTGGCAT CCAGAGGGGC CGAGCTTTTC CAGGCCACCA TTGTGAAGGC TGTGAAGGCC 
AGAATCGAGG AGGAGAAAAA GTCCATGGAC CAGCTGAAGA GGCAGATGGA CCGCATCAAG 
GCCAGGCAAC AGAAATACAA ATAGGGTAAG GAGAGGATGC TGAGCTTGAC CCAGGAGTCG 
GGGGAAGGCC AAGATCTCCA AAAACTTTCT GAAGAGGATG ACGAGAGAGA AGCAGACAAA 
CAGAAAACCA AGGGCAAAAA AAAGCAGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG 
GTCAGGAGTG GAGATTACTA TTTGTTTGAA ACGGATAGTG AAGAGGAGGA AGAGGAAGAA 
TTAAAAAAGG AAGACGAAGA GCCCCCCCGG AGGTCAGCCT TCCAGTTTGT TTATCAAGCC 
TGGATTACTG ACCCTAAAAC AGCACTCCGA CAGAGGCGCA AAGAGAAAAA AAGATCTGCA 
AGAGAAGAAC AGAAAAGAAG GAGAAAAGAA TCTGGGGAGG GTCCTGTGGA GTGGGAAGAC 
CGGGAAGATG AGCCAGTCAA AAAGAAGTCT GATGGACCAG ACAACATCAT CAAGAGGATC 
TTTAATATTT TGAAGTTCAC CTGGGTCCTG TTTCTGGCAA CCGTGGACAG TTTCACAACG 
TGGCTCAACT CCATCTCCAG AGAGCACATC GACATCTCCA CTGTCCTGAG GATCGAGCGC 
TGCATGCTGA CCAGAGAGAT CAAGAAGGGC AACGTCCCAA CCCGGGAGAG CATCCACATG 
TACTACCAGA ACCATATCAT GAACCTGTCC AGGGAGTCGG GGCTGGACAC CATCGACGAC 
CATGCTGGCG CTGCTGCGGG CGCACAGACC GCCCACAGGA TGGACAGCTT GGATTCCCAC 
GACAGCATCT CCAGCGAGCC CACGCAGTGC ACGATGCTGT ACTCGCGCCA GGGGACCACG 
GAGACCATCG AGGAAGTGGA GGCTGAGCAG GACGAGGAGA CATCCGAGGG GGCCCGGCAG 
GCCGAGTCCC CACAGGACCC CACGGAGGAT GGTGACGTGG AGGCCCCGCC GTCCTACAGC 
AAGGCTGTCA GCTTTGAACG CCTGTCCTTC GACTCCCGGG ATGACTCAGC TGGCCAGACC 
CACATGGAAG TCAGCCCGGA TGACAGCCGC TCAGATCAGC TGGAGGCCAG CATCCTGCCA 
CCCCTGACCC ACGAGCTGAC AGCCAGCGAG CTGCTGCTGA ACAAGATGTT CCATGACGAC 
GAGCTGGAGG AGTCAGAGAA GTTCTACGTG GGGCAGCCTC GCTTCCTGCT GCTCTTCTAC 
GCGCTGTACA ACACGCTGGT GGCCCGCTCC GAGATGGTGT GCTACTTTGT GATCATCCTC 
AACCACATGG TCTCCGCCTC CATGATCACC CTCGTGCTGC CCATCCTCAT CTTCCTCTGG 
GCCATGCTGT CCGTCCCCCG GCCCAGCAGG CGCTTCTGGA TGATGGCCAT CGTCTACACG 
GAGGTGGCCA TTGTGGTCAA GTACTTCTTC CAATTTGGGT TCTTCCCCTG GAACAAGAAT 
GTGGAACTGA ACAAGGACAA GCCTTACCAC CCACCCAACA TCATCGGGGT GGAGAAGAAG 
GACGCCTACG TGCTCTACGA CCTCATCCAG CTGCTTGCAC TCTTCTTCCA CCGCTCCATC 
CTAAAGTGTC ACGGCCTCTG GGATGAAGAC GACATCGTGG ACAGCGGCTC GGACAAGGGG 
GACTCGGACG ACGAGCTGTC CCTCGGCCAT GGTAGGAGGG CCTCCTCCGA CTCGCTCAAG 
TCCATCAACC TGGCTGCATC CGTGGAGTCG GTACATGTGA CCTTCCCAGA GCAGCCGGCT 
GCCGTCCGGA GGAAGCGCTC CAGCAGCGGC TCCCAGACAT CACAGGAGTC CAGCTTTTCC 
TCGAATAGGT CCAAGAGAGG CAGCACGAGC ACCCGGAACA GCAGCCAGAA AGGAAGCAGC 
GTCGTGAGCG TCAAGCAGAA GAGCAGACGG GAACTTTACA TGGAGAAGCT GCAGGAACAC 
TTAATCAAAG CAAAGGCCTT TACCATAAAG AAGACCCTGC AGCTCTACGT GCCCATCAGG 
CAGTTCTTCT ACGACCTCAT CCACCCCGAC TACAGCGCCG TCACCGACGT GTACGTGCTT 
ATGTTCCTGG CGGACACTGT GGACTTCGTC ATCATTGTCT TCGGCTTCTG GGCCTTCGGG 
AAACACTCGG CTGCAGCCGA CATCACGTCC TCACTGTCCG AGGACCAGGT GCCAGGGCCC 
TTCCTGGTGA TGGTGCTCAT TCAGTTCGGG ACTATGGTGG TGGACCGGGC GCTCTACCTC 
AGGAAGACTG TCCTGGGAAA GGTCATCTTC CAGGTCATCC TGGTGTTTGG GATTCACTTC 
TGGATGTTCT TCATCCTGCC TGGCGTGACC GAGAGGAAGT TCAGCCAGAA CCTGGTCGCG 
CAGCTGTGGT ACTTCGTCAA GTGCGTGTAC TTCGGGCTGT CCGCCTACCA GATCCGCTGC 
GGCTACCCAA CTCGGGTCCT CGGCAACTTC CTCACCAAGA GTTACAACTA CGTCAACCTC 
TTCCTGTTCC AGGGGTTCCG CCTTGTCCCT TTCCTGACGG AGCTGAGGGC TGTGATGGAC 
TGGGTGTGGA CAGACACGAC CCTGAGCCTG TCCAGCTGGA TCTGCGTGGA GGACATCTAT 
GCTCACATCT TCATCCTCAA GTGTTGGCGG GAGTCGGAAA AAAGGTACCC GCAGCCCCGG 
GGGCAGAAGA AGAAGAAGGT GGTCAAGTAC GGGATGGGGG GCATGATCAT CGTGCTGCTC 
ATCTGCATCG TCTGGTTCCC GCTACTCTTC ATGTCTCTGG TCAAATCCGT CGCTGGGGTT 
ATCAACCAAC CCCTGGACGT CTCAGTCACA ATCACCCTGG GCGGGTACCA GCCTATTTTC 
ACAATGAGTG CCCAGCAAAG CCAGCTGAAA GTTATGGACC AGCCAATGTT TAATAAATTC 
ATTAAAGCTT TTTCTAAGGA CACTGGTGCT ATGCAATTTC TGGAAAATTA TGAAAAAGAA 
GACATAACAG TCGCAGAACT GGAAGGAAAC TCAAATTCTT TATGGACCAT CAGCCCTCCC 
AGTAAGCAGA AAATGATACA CGAACTCAAG GACCCCAATA GTAGCTTCTC TGTTGTTTTT 
TCATGGAGTA TTCAGAGAAA CATGAGTCTG GGTGCAAAAG CAGAAATAGC AACAGATAAG 
CTTTCTTTCC CTCTTAAAAA CAAGACACGA GAGAATATTG CTAAAATGAT AGCTGGCGGC 
GACACGGAAA GTTCCAGGAC GCCCGTGACC ATAGAAAGGA TTTACCCGTA CTATGTGAAA 
GCACCCAGTG ACTCTAACTC CAAACCCATA AAGCAGCTTT TATCTGAAAA TAATTTCATG 
AACATCACCA TCATTTTGTC CAGAGACAAT GCGACTAACT CGAACAGTGA GTGGTGGGTT 
CTCAACTTGA CTGGGAACAG AATCTACAAC CAGGAAGCGC AGGCCCTGGA GCTGGTGGTC 
TTCAACGACA AGGTCAGCCC CCCCAGCCTG GGGTTCCTGG CCGGCTACGG TATCATGGGC 
CTCTACGCTT CCGTGGTGCT GGTGATTGGG AAGTTTGTCC GGGAATTCTT CAGCGGGATT 
TCTCACTCCA TCATGTTTGA AGAACTTCCC AATGTGGATC GGATTCTGAA ATTGTGCACA 
GACATTTTTT TAGTCCGGGA GACCGGAGAG CTGGAGCTCG AGGAGGATCT CTATGCCAAA 
TTAATATTCC TGTACCGGTC GCCAGAAACC ATGATCAAAT GGACCAGGGA GAAAACAAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_012103587.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011958977.1 Ovis aries piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

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CloneID	OSm26167
Clone ID Related Accession (Same CDS sequence)	XM_012103587.2
Accession Version	XM_012103587.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	piezo-type mechanosensitive ion channel component 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639032.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012103587.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTGG CACCCCAGTC CTGGAGACCC TTTGCTCGGA CTCGCGGGAC CGACGCGATG 
GCCTCGGAAG TGGTGTGCGG GCTCGTCTTC AGGCTGCTGC TCCCCATCTG CCTGGCAGTA 
GCCTGTGCCT TCCGGTACAA CGGGCTCTCC TTCGTCTACC TCATCTACCT CCTGCTCATC 
CCCCTGTTCT CGGAACCAAC TAAAGCCACC ATGCAAGGAC ACACGGGACG GTTGTTAAAG 
TCTCTGTGCT TCACCAGTCT CTCCTTCCTG TTGCTGCACA TCATTTTCCA CATCACGTTG 
GCGAGTCTGG AGGCACAGCA CCGCATTGCA CCTGGTTACA ACTGCTCAAC CTGGGAGAAG 
ACTTTCCGAC AAATCGGCTT CGAAAGCTTA AAGGGAGCCG ACGCTGGCAA TGGGATCCGA 
GTGTTTGTGC CTGACGTGGG CATGTTCATC GCCAGCCTGA CCATCTGGCT GGTCTGTAGG 
AACATTGTTC AGAAGCCAGT GGCCGAAGAG GCCGCGCAGT GTAACCCGGA GTTTGAGAAC 
GAAGAGTCGG CTGGAGGAGA AAAAATAGAC TCCGAAGAGG CCCTGATCTA TGAAGAGGAC 
TTAGATGAAG GAGACGGTGC TGAAGGCGAT TTGGAGGAAA GCACGAAATT AAAACTCTTC 
CGCAGGCTGG CCTCCGTGGC CTCCAAGCTG AAGGAGTTTA TTGGCAACAT GATAACTACA 
GCGGGGAAGG TTGTTGTGAC AATTTTACTG GGTTCATCAG GGATGATGCT GCCCTCTCTG 
ACCTCATCCG TGTACTTCTT CGTGTTCCTG GGCCTGTGTA CCTGGTGGTC CTGGTGCCGG 
ACCTTCGACC CCTTGCTGTT CAGCTGTCTC TGCGTCCTGC TGGCCATCTT CACTGCGGGA 
CACCTGATCG GACTCTATCT GTACCAGTTC CAGTTCTTCC AGGAAGCCGT TCCCCCCAAC 
GACTACTATG CCAGGTTGTT TGGTATCAAA TCAGTCATTC AGACGGACTG TTCCAGCACA 
TGGAAGATCA TTGCCAACCC CGACCTGTCC TGGTACCACC ACGCCAACCC CATCGTCCTG 
CTGGTGATGT ACTACACGCT GGCTACTCTG ATCCGAATCT GGCTGCAGGA GCCCGTTGTA 
AGGACCAGAA GTTTTCATGG TCTTGTCTGC AAAGTAGCAG GAGACTCCGG GGAAAGGGTG 
CAGGAAGAGA AGACCAAGGA GGAGGACAGA GCCCTGGCCT GCAGCCCCGC GCCCATCACG 
GCCGAGAGGA GGCGGAGCCT GTGGTACGCG ACCCACTACC CCACCGACGA GAGAAAACTT 
TTATCCATGA CTCAGGATGA CTACAAGCCG TCCGACGGCC TGCTGGTGAC CGTGAATGGC 
AACCCCGTGG ACTACCACAC CATCCACCCC AGCCTCCCAC TGGAGAACGG CCCCGCCAAG 
GCTGACCTGT ACTCCACACC ACAGTACCGC TGGGAGCCTG CCACTGCCTC CCCTGAAAAG 
AAGGAGGAAG ATAATGATGA GAAAGAAGAG TTTGAGGAGG AGCGGAGCCG GGAGGAGAAG 
AGGAGTGTCA AAGTGCACGC CATGGTGTCT GTGTTCCAGT TTATCATGAA ACAGAGCTAC 
ATCTGTGCCC TCATCGCCAT GATGGCCTGG AGCATCACCT ACCACAGCTG GCTGACCTTC 
GTGCTGCTCA TCTGGTCCTG CACCCTGTGG ATGATCCGCA ACCGGAGGAA GTATGCCATG 
ATCAGCTCCC CCTTCATGGT CGTTTACGGA AACCTGCTGC TCATACTGCA GTACATATGG 
AGCTTCGAGC TCCCTGAGAT TAAAAAGGTC CCGGGTTTCT TAGAAAAGAA GGAGCCGGGG 
GAGCTCGCCT CAAAGATTCT GTTCACCATC ACATTCTGGC TGCTGCTGAG ACAGCACCTC 
ACAGAGCAGA AAGCTCTCCA AGAAAAGGAA GCTCTTCTGT CTGAGGTCAA AATCGGAAGT 
CAGGAAAATG AAGAAAAAGA TGATGAACAG GATGTACAGG TAGACAGCGA GGCTCAGGAG 
GAGGAAGGAG AGGAGCCGAA GGAGGAGCAG CAGGAGCGGA GAGAGGAAGA GGAGGAGGAA 
GAGGCAGACG AGCAGGACGT GATGAAGGTG CTGGGGAACC TGGTGGTGGC CCTGTTCATC 
AAGTACTGGA TCTACGTCTG CGGGGGGATG TTCTTCTTCG TCAGCTTCGA GGGTAAAATC 
GTGATGTACA AGATCATCTA CATGGTACTC TTCCTCTTCT GTGTGGCCCT GTATCAGGTG 
CACTATGAAT GGTGGAGGAG GATTCTGAAG TACTTCTGGA TGTCGGTGGT TATCTACACA 
ATGCTGGTGC TTATCTTTAT ATACACATAT CAGTTTGAAA ACTTCCCAGG CTTGTGGCAA 
AACATGACTG GACTGAAAAA AGAAAAGCTG GAGGACCTTG GCCTGAAGCA GTTCACGGTG 
GCCGAGCTGT TCACGCGCAT CTTCATCCCC ACCTCCTTCC TCCTGGTCTG CATTCTGCAC 
CTGCACTACT TCCACGACCG ATTCCTGGAG CTCACCGACC TGAAGTCCAT CCCCAGCAAG 
GAGGACAGCG CTGTCTATAG GCTGGCCCAC CCCGAGGGAA GCCTTCCAGA CCTTGCCATG 
ATGAAACTGA CTGCCAGCCT GGAGGTGGCA GAGGTCAAGC AGCTGGCTGG GCCTGGGGAG 
GAGATGCCAG GGGGATGCCC TGCAAAAGCC AAGAAGGGCG AGCCTGGCAG GGACAGCGAG 
GACGAGGATG AGGATGAGGA CGAGGAGGAT GAAGAGGAGG AGGAGACGTC AGATCTGAGG 
AACAAGTGGC ACCTGGTGAT CGACCGCCTC ACAGTGCTGC TGCTGCGATT CCTGGAGGGC 
TTCCACCAGC TGCAGACATT CCTGTGGTGG ATCCTGGAGC TGCACATCGT CAAGATTGTG 
TCCTCCTACA TCATCTGGGT GTCCGTGAAG GAGGTGTCTC TGTTCAACTA CGTGTTTCTG 
ATTTCCTGGG CTTTTGCTCT GCCGTATGCT AAGCTCCGCC GTCTGGCCTC CAGTGTCTGC 
ACCGTCTGGA CATGTGTGAT CATTGTCTGT AAGATGCTGT ACCAGCTGCA GACCATCAAG 
CCTGAGAACT TCTCTGTGAA CTGTTCGTTG CCAAATGAAA ACCAGACGAA CATCCCCCTG 
CACCAGCTGA ACAAGTCTCT GCTCTACAGC GCACCCATTG ACCCCACAGA ATGGGTGGGC 
CTCAGGAAGT CGTCTCCTCT ACTTGTCTAC CTGAGGAACA ACCTGCTGAT GCTGGCCATC 
CTGGCCTTCG AGGTCACCAT CTACCGCCAT CAGGAGTACT ACCGTGGTCG GAACAACCTC 
TCAACCCCCG TGTCTAAAAC CATCTTCCAC AACATCACAA GACTCCAGCT GGATGATGGA 
CTTGTTAACT GTGCCAAGTA TTTCATCAAT TACTTCTTCT ACAAGTTTGG TCTGGAGACC 
TGTTTCCTCA TGTCAGTTAA TGTGATTGGC CAGCGCATGG ACTTCTACGC CATGATTCAC 
GCCTGCTGGC TGATTGCCGT CCTGTACCGG CGCAGGAGGA AGGCCATCGC CGAGATCTGG 
CCCAAGTACT GCTGCTTCCT GGCTTGCATC ATCACCTTCC AGTATTTCAT CTGCATCGGC 
ATCCCCCCTG CCCCCTGTCG AGACTACCCT TGGAGGTTCA AGGGCGCCAG CTTCAATGAC 
AACATCGTCA AGTGGCTGTA CTTCCCTGAC TTCATCGTTC GACCCAACCC TGTGTTCCTC 
GTGTATGATT TCATGCTACT CCTGTGTGCC TCGCTGCAAC GGCAGATCTT TGAGGATGAG 
AATAAGGCTG CTGTACGGAT CGTGGCGGGC GACAACGTGG AAATCTGCAT GAACCTGGAT 
GCGGCCTCAT TTAGCCAGCA CAATCCCGTG CCCGACTTCA TCCACTGCAG GTCGTACCTG 
GACATGTCCA AGGTGGTCAT CTTCAGCTAC CTCTTCTGGT TCGTGCTCAC TATCATCTTC 
ATCACCGGGA CGACTCGCAT CAGCATCTTT TGCATGGGCT ACCTGGTGGC CTGCTTCTAC 
TTCCTGCTCT TCGGGGGCGA CTTGCTGCTG AAACCCATCA AGAGCATCCT GCGCTACTGG 
GACTGGCTGA TCGCTTACAA TGTCTTCGTG ATCACGATGA AAAACATACT GTCGATAGGA 
GCGTGTGGCT ATATTGAAAA ATTGGTTCAA AATAGTTGCT GGTTGATCCA AGCATTCAGC 
CTGGCCTGTA CAGTCAAGGG CTACAAAATG CCTGATGATG ACTCCTCCTG TAAACTGCCC 
AGTGGGGAGG CAGGAATCAT TTGGGACAGC ATATGCTTTG CCTTCCTGCT GCTGCAGAGA 
AGAGTGTTCA TGAGTTACTA TTTCCTGCAT GTGGTGGCAG ATATAAAAGC CTCACAGATC 
CTGGCATCCA GAGGGGCCGA GCTTTTCCAG GCCACCATTG TGAAGGCTGT GAAGGCCAGA 
ATCGAGGAGG AGAAAAAGTC CATGGACCAG CTGAAGAGGC AGATGGACCG CATCAAGGCC 
AGGCAACAGA AATACAAAAA GGGTAAGGAG AGGATGCTGA GCTTGACCCA GGAGTCGGGG 
GAAGGCCAAG ATCTCCAAAA ACTTTCTGAA GAGGATGACG AGAGAGAAGC AGACAAACAG 
AAAACCAAGG GCAAAAAAAA GCAGTGGTGG CGGCCTTGGG TTGATCATGT TTCCATGGTC 
AGGAGTGGAG ATTACTATTT GTTTGAAACG GATAGTGAAG AGGAGGAAGA GGAAGAATTA 
AAAAAGGAAG ACGAAGAGCC CCCCCGGAGG TCAGCCTTCC AGTTTGTTTA TCAAGCCTGG 
ATTACTGACC CTAAAACAGC ACTCCGACAG AGGCGCAAAG AGAAAAAAAG ATCTGCAAGA 
GAAGAACAGA AAAGAAGGAG AAAAGTATCT GGGGAGGGTC CTGTGGAGTG GGAAGACCGG 
GAAGATGAGC CAGTCAAAAA GAAGTCTGAT GGACCAGACA ACATCATCAA GAGGATCTTT 
AATATTTTGA AGTTCACCTG GGTCCTGTTT CTGGCAACCG TGGACAGTTT CACAACGTGG 
CTCAACTCCA TCTCCAGAGA GCACATCGAC ATCTCCACTG TCCTGAGGAT CGAGCGCTGC 
ATGCTGACCA GAGAGATCAA GAAGGGCAAC GTCCCAACCC GGGAGAGCAT CCACATGTAC 
TACCAGAACC ATATCATGAA CCTGTCCAGG GAGTCGGGGC TGGACACCAT CGACGACCAT 
GCTGGCGCTG CTGCGGGCGC ACAGACCGCC CACAGGATGG ACAGCTTGGA TTCCCACGAC 
AGCATCTCCA GCGAGCCCAC GCAGTGCACG ATGCTGTACT CGCGCCAGGG GACCACGGAG 
ACCATCGAGG AAGTGGAGGC TGAGCAGGAC GAGGAGACAT CCGAGGGGGC CCGGCAGGCC 
GAGTCCCCAC AGGACCCCAC GGAGGATGGT GACGTGGAGG CCCCGCCGTC CTACAGCAAG 
GCTGTCAGCT TTGAACGCCT GTCCTTCGAC TCCCGGGATG ACTCAGCTGG CCAGACCCAC 
ATGGAAGTCA GCCCGGATGA CAGCCGCTCA GATCGGCTGG AGGCCAGCAT CCTGCCACCC 
CTGACCCACG AGCTGACAGC CAGCGAGCTG CTGCTGAACA AGATGTTCCA TGACGACGAG 
CTGGAGGAGT CAGAGAAGTT CTACGTGGGG CAGCCTCGCT TCCTGCTGCT CTTCTACGCG 
CTGTACAACA CGCTGGTGGC CCGCTCCGAG ATGGTGTGCT ACTTTGTGAT CATCCTCAAC 
CACATGGTCT CCGCCTCCAT GATCACCCTC GTGCTGCCCA TCCTCATCTT CCTCTGGGCC 
ATGCTGTCCG TCCCCCGGCC CAGCAGGCGC TTCTGGATGA TGGCCATCGT CTACACGGAG 
GTGGCCATTG TGGTCAAGTA CTTCTTCCAA TTTGGGTTCT TCCCCTGGAA CAAGAATGTG 
GAACTGAACA AGGACAAGCC TTACCACCCA CCCAACATCA TCGGGGTGGA GAAGAAGGAC 
GCCTACGTGC TCTACGACCT CATCCAGCTG CTCGCACTCT TCTTCCACCG CTCCATCCTA 
AAGTGTCACG GCCTCTGGGA TGAAGACGAC ATCGTGGACA GCGGCTCGGA CAAGGTGGAC 
TCGGACGACG AGCTGTCCCT CGGCCATGGT AGGAGGGCCT CCTCCGACTC GCTCAAGTCC 
ATCAACCTGG CTGCATCCGT GGAGTCGGTA CACGTGACCT TCCCAGAGCA GCCGGCTGCC 
GTCCGGAGGA AGCGCTCCAG CAGCGGCTCC CAGACATCAC AGGAGTCCAG CTTTTCCTCG 
AATAGGTCCA AGAGAGGCAG CACGAGCACC CGGAACAGCA GCCAGAAAGG AAGCAGCGTC 
GTGAGCGTCA AGCAGAAGAG CAGACGGGAA CTTTACATGG AGAAGCTGCA GGAACACTTA 
ATCAAAGCAA AGGCCTTTAC CATAAAGAAG ACCCTGCAGC TCTACGTGCC CATCAGGCAG 
TTCTTCTACG ACCTCATCCA CCCCGACTAC AGCGCCGTCA CCGACGTGTA CGTGCTTATG 
TTCCTGGCGG ACACCGTGGA CTTCATCATC ATTGTCTTCG GCTTCTGGGC CTTCGGGAAA 
CACTCGGCTG CAGCCGACAT CACGTCCTCA CTGTCCGAGG ACCAGGTGCC AGGGCCCTTC 
CTGGTGATGG TGCTCATTCA GTTCGGGACT ATGGTGGTGG ACCGGGCGCT CTACCTCAGG 
AAGACTGTCC TGGGAAAGGT CATCTTCCAG GTCATCCTGG TGTTTGGGAT TCACTTCTGG 
ATGTTCTTCA TCCTGCCTGG CGTGACCGAG AGGAAGTTCA GCCAGAACCT GGTCGCGCAG 
CTGTGGTACT TCGTCAAGTG CGTGTACTTC GGGCTGTCCG CCTACCAGAT CCGCTGCGGC 
TACCCAACTC GGGTCCTCGG CAACTTCCTC ACCAAGAGTT ACAACTACGT CAACCTCTTC 
CTGTTCCAGG GGTTCCGCCT TGTCCCTTTC CTGACGGAGC TGAGGGCTGT GATGGACTGG 
GTGTGGACAG ACACGACCCT GAGCCTGTCC AGCTGGATCT GCGTGGAGGA CATCTATGCT 
CACATCTTCA TCCTCAAGTG TTGGCGGGAG TCGGAAAAAA GGTACCCGCA GCCCCGGGGG 
CAGAAGAAGA AGAAGGTGGT CAAGTACGGG ATGGGGGGCA TGATCATCGT GCTGCTCATC 
TGCATCGTCT GGTTCCCGCT ACTCTTCATG TCTCTGGTCA AATCCGTCGC TGGGGTTATC 
AACCAACCCC TGGACGTCTC AGTCACAATC ACCCTGGGCG GGTACCAGCC TATTTTCACA 
ATGAGTGCCC AGCAAAGCCA GCTGAAAGTT ATGGACCAGC CAATGTTTAA TAAATTCATT 
AAAGCTTTTT CTAAGGACAC TGGTGCTATG CAATTTCTGG AAAATTATGA AAAAGAAGAC 
ATAACAGTCG CAGAACTGGA AGGAAACTCA AATTCTTTAT GGACCATCAG CCCTCCCAGT 
AAGCAGAAAA TGATACACGA ACTCAAGGAC CCCAATAGTA GCTTCTCTGT TGTTTTTTCA 
TGGAGTATTC AGAGAAACAT GAGTCTGGGT GCAAAAGCAG AAATAGCAAC AGATAAGCTT 
TCTTTCCCTC TTAAAAACAA GACACGAGAG AATATTGCTA AAATGATAGC TGGCGGCGAC 
ACGGAAAGTT CCAGGACGCC CGTGACCATA GAAAGGATTT ACCCGTACTA TGTGAAAGCA 
CCCAGTGACT CTAACTCCAA ACCCATAAAG CAGCTTTTAT CTGAAAATAA TTTCATGAAC 
ATCACCATCA TTTTGTCCAG AGACAATGCG ACTAACTCGA ACAGTGAGTG GTGGGTTCTC 
AACTTGACTG GGAACAGAAT CTACAACCAG GAAGCGCAGG CCCTGGAGCT GGTGGTCTTC 
AACGACAAGG TCAGCCCCCC CAGCCTGGGG TTCCTGGCCG GCTACGGTAT CATGGGCCTC 
TACGCTTCCG TGGTGCTGGT GATTGGGAAG TTTGTCCGGG AATTCTTCAG CGGGATTTCT 
CACTCCATCA TGTTTGAAGA ACTTCCCAAT GTGGATCGGA TTCTGAAATT GTGCACAGAC 
ATTTTTTTAG TCCGGGAGAC CGGAGAGCTG GAGCTCGAGG AGGATCTCTA TGCCAAATTA 
ATATTCCTGT ACCGGTCGCC AGAAACCATG ATCAAATGGA CCAGGGAGAA AACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011958977.1
CDS	747..9026
Translation	MGLAPQSWRPFARTRGTDAMASEVVCGLVFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKATMQGHTGRLLKSLCFTSLSFLLLHIIFHITLASLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDVGMFIASLTIWLVCRNIVQKPVAEEAAQCNPEFENEESAGGEKIDSEEALIYEEDLDEGDGAEGDLEESTKLKLFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIANPDLSWYHHANPIVLLVMYYTLATLIRIWLQEPVVRTRSFHGLVCKVAGDSGERVQEEKTKEEDRALACSPAPITAERRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPLENGPAKADLYSTPQYRWEPATASPEKKEEDNDEKEEFEEERSREEKRSVKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDDEQDVQVDSEAQEEEGEEPKEEQQERREEEEEEEADEQDVMKVLGNLVVALFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRRILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDSAVYRLAHPEGSLPDLAMMKLTASLEVAEVKQLAGPGEEMPGGCPAKAKKGEPGRDSEDEDEDEDEEDEEEEETSDLRNKWHLVIDRLTVLLLRFLEGFHQLQTFLWWILELHIVKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPLHQLNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLSTPVSKTIFHNITRLQLDDGLVNCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIVKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIVAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVVIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIEKLVQNSCWLIQAFSLACTVKGYKMPDDDSSCKLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQESGEGQDLQKLSEEDDEREADKQKTKGKKKQWWRPWVDHVSMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRRKEKKRSAREEQKRRRKVSGEGPVEWEDREDEPVKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDDHAGAAAGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQDEETSEGARQAESPQDPTEDGDVEAPPSYSKAVSFERLSFDSRDDSAGQTHMEVSPDDSRSDRLEASILPPLTHELTASELLLNKMFHDDELEESEKFYVGQPRFLLLFYALYNTLVARSEMVCYFVIILNHMVSASMITLVLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVELNKDKPYHPPNIIGVEKKDAYVLYDLIQLLALFFHRSILKCHGLWDEDDIVDSGSDKVDSDDELSLGHGRRASSDSLKSINLAASVESVHVTFPEQPAAVRRKRSSSGSQTSQESSFSSNRSKRGSTSTRNSSQKGSSVVSVKQKSRRELYMEKLQEHLIKAKAFTIKKTLQLYVPIRQFFYDLIHPDYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLVKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQPMFNKFIKAFSKDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELKDPNSSFSVVFSWSIQRNMSLGAKAEIATDKLSFPLKNKTRENIAKMIAGGDTESSRTPVTIERIYPYYVKAPSDSNSKPIKQLLSENNFMNITIILSRDNATNSNSEWWVLNLTGNRIYNQEAQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_012103587.2
Organism	Ovis aries(sheep)
Definition	Ovis aries piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012103587.2

ORF Insert Sequence:

ATGGGCTTGG CACCCCAGTC CTGGAGACCC TTTGCTCGGA CTCGCGGGAC CGACGCGATG 
GCCTCGGAAG TGGTGTGCGG GCTCGTCTTC AGGCTGCTGC TCCCCATCTG CCTGGCAGTA 
GCCTGTGCCT TCCGGTACAA CGGGCTCTCC TTCGTCTACC TCATCTACCT CCTGCTCATC 
CCCCTGTTCT CGGAACCAAC TAAAGCCACC ATGCAAGGAC ACACGGGACG GTTGTTAAAG 
TCTCTGTGCT TCACCAGTCT CTCCTTCCTG TTGCTGCACA TCATTTTCCA CATCACGTTG 
GCGAGTCTGG AGGCACAGCA CCGCATTGCA CCTGGTTACA ACTGCTCAAC CTGGGAGAAG 
ACTTTCCGAC AAATCGGCTT CGAAAGCTTA AAGGGAGCCG ACGCTGGCAA TGGGATCCGA 
GTGTTTGTGC CTGACGTGGG CATGTTCATC GCCAGCCTGA CCATCTGGCT GGTCTGTAGG 
AACATTGTTC AGAAGCCAGT GGCCGAAGAG GCCGCGCAGT GTAACCCGGA GTTTGAGAAC 
GAAGAGTCGG CTGGAGGAGA AAAAATAGAC TCCGAAGAGG CCCTGATCTA TGAAGAGGAC 
TTAGATGAAG GAGACGGTGC TGAAGGCGAT TTGGAGGAAA GCACGAAATT AAAACTCTTC 
CGCAGGCTGG CCTCCGTGGC CTCCAAGCTG AAGGAGTTTA TTGGCAACAT GATAACTACA 
GCGGGGAAGG TTGTTGTGAC AATTTTACTG GGTTCATCAG GGATGATGCT GCCCTCTCTG 
ACCTCATCCG TGTACTTCTT CGTGTTCCTG GGCCTGTGTA CCTGGTGGTC CTGGTGCCGG 
ACCTTCGACC CCTTGCTGTT CAGCTGTCTC TGCGTCCTGC TGGCCATCTT CACTGCGGGA 
CACCTGATCG GACTCTATCT GTACCAGTTC CAGTTCTTCC AGGAAGCCGT TCCCCCCAAC 
GACTACTATG CCAGGTTGTT TGGTATCAAA TCAGTCATTC AGACGGACTG TTCCAGCACA 
TGGAAGATCA TTGCCAACCC CGACCTGTCC TGGTACCACC ACGCCAACCC CATCGTCCTG 
CTGGTGATGT ACTACACGCT GGCTACTCTG ATCCGAATCT GGCTGCAGGA GCCCGTTGTA 
AGGACCAGAA GTTTTCATGG TCTTGTCTGC AAAGTAGCAG GAGACTCCGG GGAAAGGGTG 
CAGGAAGAGA AGACCAAGGA GGAGGACAGA GCCCTGGCCT GCAGCCCCGC GCCCATCACG 
GCCGAGAGGA GGCGGAGCCT GTGGTACGCG ACCCACTACC CCACCGACGA GAGAAAACTT 
TTATCCATGA CTCAGGATGA CTACAAGCCG TCCGACGGCC TGCTGGTGAC CGTGAATGGC 
AACCCCGTGG ACTACCACAC CATCCACCCC AGCCTCCCAC TGGAGAACGG CCCCGCCAAG 
GCTGACCTGT ACTCCACACC ACAGTACCGC TGGGAGCCTG CCACTGCCTC CCCTGAAAAG 
AAGGAGGAAG ATAATGATGA GAAAGAAGAG TTTGAGGAGG AGCGGAGCCG GGAGGAGAAG 
AGGAGTGTCA AAGTGCACGC CATGGTGTCT GTGTTCCAGT TTATCATGAA ACAGAGCTAC 
ATCTGTGCCC TCATCGCCAT GATGGCCTGG AGCATCACCT ACCACAGCTG GCTGACCTTC 
GTGCTGCTCA TCTGGTCCTG CACCCTGTGG ATGATCCGCA ACCGGAGGAA GTATGCCATG 
ATCAGCTCCC CCTTCATGGT CGTTTACGGA AACCTGCTGC TCATACTGCA GTACATATGG 
AGCTTCGAGC TCCCTGAGAT TAAAAAGGTC CCGGGTTTCT TAGAAAAGAA GGAGCCGGGG 
GAGCTCGCCT CAAAGATTCT GTTCACCATC ACATTCTGGC TGCTGCTGAG ACAGCACCTC 
ACAGAGCAGA AAGCTCTCCA AGAAAAGGAA GCTCTTCTGT CTGAGGTCAA AATCGGAAGT 
CAGGAAAATG AAGAAAAAGA TGATGAACAG GATGTACAGG TAGACAGCGA GGCTCAGGAG 
GAGGAAGGAG AGGAGCCGAA GGAGGAGCAG CAGGAGCGGA GAGAGGAAGA GGAGGAGGAA 
GAGGCAGACG AGCAGGACGT GATGAAGGTG CTGGGGAACC TGGTGGTGGC CCTGTTCATC 
AAGTACTGGA TCTACGTCTG CGGGGGGATG TTCTTCTTCG TCAGCTTCGA GGGTAAAATC 
GTGATGTACA AGATCATCTA CATGGTACTC TTCCTCTTCT GTGTGGCCCT GTATCAGGTG 
CACTATGAAT GGTGGAGGAG GATTCTGAAG TACTTCTGGA TGTCGGTGGT TATCTACACA 
ATGCTGGTGC TTATCTTTAT ATACACATAT CAGTTTGAAA ACTTCCCAGG CTTGTGGCAA 
AACATGACTG GACTGAAAAA AGAAAAGCTG GAGGACCTTG GCCTGAAGCA GTTCACGGTG 
GCCGAGCTGT TCACGCGCAT CTTCATCCCC ACCTCCTTCC TCCTGGTCTG CATTCTGCAC 
CTGCACTACT TCCACGACCG ATTCCTGGAG CTCACCGACC TGAAGTCCAT CCCCAGCAAG 
GAGGACAGCG CTGTCTATAG GCTGGCCCAC CCCGAGGGAA GCCTTCCAGA CCTTGCCATG 
ATGAAACTGA CTGCCAGCCT GGAGGTGGCA GAGGTCAAGC AGCTGGCTGG GCCTGGGGAG 
GAGATGCCAG GGGGATGCCC TGCAAAAGCC AAGAAGGGCG AGCCTGGCAG GGACAGCGAG 
GACGAGGATG AGGATGAGGA CGAGGAGGAT GAAGAGGAGG AGGAGACGTC AGATCTGAGG 
AACAAGTGGC ACCTGGTGAT CGACCGCCTC ACAGTGCTGC TGCTGCGATT CCTGGAGGGC 
TTCCACCAGC TGCAGACATT CCTGTGGTGG ATCCTGGAGC TGCACATCGT CAAGATTGTG 
TCCTCCTACA TCATCTGGGT GTCCGTGAAG GAGGTGTCTC TGTTCAACTA CGTGTTTCTG 
ATTTCCTGGG CTTTTGCTCT GCCGTATGCT AAGCTCCGCC GTCTGGCCTC CAGTGTCTGC 
ACCGTCTGGA CATGTGTGAT CATTGTCTGT AAGATGCTGT ACCAGCTGCA GACCATCAAG 
CCTGAGAACT TCTCTGTGAA CTGTTCGTTG CCAAATGAAA ACCAGACGAA CATCCCCCTG 
CACCAGCTGA ACAAGTCTCT GCTCTACAGC GCACCCATTG ACCCCACAGA ATGGGTGGGC 
CTCAGGAAGT CGTCTCCTCT ACTTGTCTAC CTGAGGAACA ACCTGCTGAT GCTGGCCATC 
CTGGCCTTCG AGGTCACCAT CTACCGCCAT CAGGAGTACT ACCGTGGTCG GAACAACCTC 
TCAACCCCCG TGTCTAAAAC CATCTTCCAC AACATCACAA GACTCCAGCT GGATGATGGA 
CTTGTTAACT GTGCCAAGTA TTTCATCAAT TACTTCTTCT ACAAGTTTGG TCTGGAGACC 
TGTTTCCTCA TGTCAGTTAA TGTGATTGGC CAGCGCATGG ACTTCTACGC CATGATTCAC 
GCCTGCTGGC TGATTGCCGT CCTGTACCGG CGCAGGAGGA AGGCCATCGC CGAGATCTGG 
CCCAAGTACT GCTGCTTCCT GGCTTGCATC ATCACCTTCC AGTATTTCAT CTGCATCGGC 
ATCCCCCCTG CCCCCTGTCG AGACTACCCT TGGAGGTTCA AGGGCGCCAG CTTCAATGAC 
AACATCGTCA AGTGGCTGTA CTTCCCTGAC TTCATCGTTC GACCCAACCC TGTGTTCCTC 
GTGTATGATT TCATGCTACT CCTGTGTGCC TCGCTGCAAC GGCAGATCTT TGAGGATGAG 
AATAAGGCTG CTGTACGGAT CGTGGCGGGC GACAACGTGG AAATCTGCAT GAACCTGGAT 
GCGGCCTCAT TTAGCCAGCA CAATCCCGTG CCCGACTTCA TCCACTGCAG GTCGTACCTG 
GACATGTCCA AGGTGGTCAT CTTCAGCTAC CTCTTCTGGT TCGTGCTCAC TATCATCTTC 
ATCACCGGGA CGACTCGCAT CAGCATCTTT TGCATGGGCT ACCTGGTGGC CTGCTTCTAC 
TTCCTGCTCT TCGGGGGCGA CTTGCTGCTG AAACCCATCA AGAGCATCCT GCGCTACTGG 
GACTGGCTGA TCGCTTACAA TGTCTTCGTG ATCACGATGA AAAACATACT GTCGATAGGA 
GCGTGTGGCT ATATTGAAAA ATTGGTTCAA AATAGTTGCT GGTTGATCCA AGCATTCAGC 
CTGGCCTGTA CAGTCAAGGG CTACAAAATG CCTGATGATG ACTCCTCCTG TAAACTGCCC 
AGTGGGGAGG CAGGAATCAT TTGGGACAGC ATATGCTTTG CCTTCCTGCT GCTGCAGAGA 
AGAGTGTTCA TGAGTTACTA TTTCCTGCAT GTGGTGGCAG ATATAAAAGC CTCACAGATC 
CTGGCATCCA GAGGGGCCGA GCTTTTCCAG GCCACCATTG TGAAGGCTGT GAAGGCCAGA 
ATCGAGGAGG AGAAAAAGTC CATGGACCAG CTGAAGAGGC AGATGGACCG CATCAAGGCC 
AGGCAACAGA AATACAAAAA GGGTAAGGAG AGGATGCTGA GCTTGACCCA GGAGTCGGGG 
GAAGGCCAAG ATCTCCAAAA ACTTTCTGAA GAGGATGACG AGAGAGAAGC AGACAAACAG 
AAAACCAAGG GCAAAAAAAA GCAGTGGTGG CGGCCTTGGG TTGATCATGT TTCCATGGTC 
AGGAGTGGAG ATTACTATTT GTTTGAAACG GATAGTGAAG AGGAGGAAGA GGAAGAATTA 
AAAAAGGAAG ACGAAGAGCC CCCCCGGAGG TCAGCCTTCC AGTTTGTTTA TCAAGCCTGG 
ATTACTGACC CTAAAACAGC ACTCCGACAG AGGCGCAAAG AGAAAAAAAG ATCTGCAAGA 
GAAGAACAGA AAAGAAGGAG AAAAGTATCT GGGGAGGGTC CTGTGGAGTG GGAAGACCGG 
GAAGATGAGC CAGTCAAAAA GAAGTCTGAT GGACCAGACA ACATCATCAA GAGGATCTTT 
AATATTTTGA AGTTCACCTG GGTCCTGTTT CTGGCAACCG TGGACAGTTT CACAACGTGG 
CTCAACTCCA TCTCCAGAGA GCACATCGAC ATCTCCACTG TCCTGAGGAT CGAGCGCTGC 
ATGCTGACCA GAGAGATCAA GAAGGGCAAC GTCCCAACCC GGGAGAGCAT CCACATGTAC 
TACCAGAACC ATATCATGAA CCTGTCCAGG GAGTCGGGGC TGGACACCAT CGACGACCAT 
GCTGGCGCTG CTGCGGGCGC ACAGACCGCC CACAGGATGG ACAGCTTGGA TTCCCACGAC 
AGCATCTCCA GCGAGCCCAC GCAGTGCACG ATGCTGTACT CGCGCCAGGG GACCACGGAG 
ACCATCGAGG AAGTGGAGGC TGAGCAGGAC GAGGAGACAT CCGAGGGGGC CCGGCAGGCC 
GAGTCCCCAC AGGACCCCAC GGAGGATGGT GACGTGGAGG CCCCGCCGTC CTACAGCAAG 
GCTGTCAGCT TTGAACGCCT GTCCTTCGAC TCCCGGGATG ACTCAGCTGG CCAGACCCAC 
ATGGAAGTCA GCCCGGATGA CAGCCGCTCA GATCGGCTGG AGGCCAGCAT CCTGCCACCC 
CTGACCCACG AGCTGACAGC CAGCGAGCTG CTGCTGAACA AGATGTTCCA TGACGACGAG 
CTGGAGGAGT CAGAGAAGTT CTACGTGGGG CAGCCTCGCT TCCTGCTGCT CTTCTACGCG 
CTGTACAACA CGCTGGTGGC CCGCTCCGAG ATGGTGTGCT ACTTTGTGAT CATCCTCAAC 
CACATGGTCT CCGCCTCCAT GATCACCCTC GTGCTGCCCA TCCTCATCTT CCTCTGGGCC 
ATGCTGTCCG TCCCCCGGCC CAGCAGGCGC TTCTGGATGA TGGCCATCGT CTACACGGAG 
GTGGCCATTG TGGTCAAGTA CTTCTTCCAA TTTGGGTTCT TCCCCTGGAA CAAGAATGTG 
GAACTGAACA AGGACAAGCC TTACCACCCA CCCAACATCA TCGGGGTGGA GAAGAAGGAC 
GCCTACGTGC TCTACGACCT CATCCAGCTG CTCGCACTCT TCTTCCACCG CTCCATCCTA 
AAGTGTCACG GCCTCTGGGA TGAAGACGAC ATCGTGGACA GCGGCTCGGA CAAGGTGGAC 
TCGGACGACG AGCTGTCCCT CGGCCATGGT AGGAGGGCCT CCTCCGACTC GCTCAAGTCC 
ATCAACCTGG CTGCATCCGT GGAGTCGGTA CACGTGACCT TCCCAGAGCA GCCGGCTGCC 
GTCCGGAGGA AGCGCTCCAG CAGCGGCTCC CAGACATCAC AGGAGTCCAG CTTTTCCTCG 
AATAGGTCCA AGAGAGGCAG CACGAGCACC CGGAACAGCA GCCAGAAAGG AAGCAGCGTC 
GTGAGCGTCA AGCAGAAGAG CAGACGGGAA CTTTACATGG AGAAGCTGCA GGAACACTTA 
ATCAAAGCAA AGGCCTTTAC CATAAAGAAG ACCCTGCAGC TCTACGTGCC CATCAGGCAG 
TTCTTCTACG ACCTCATCCA CCCCGACTAC AGCGCCGTCA CCGACGTGTA CGTGCTTATG 
TTCCTGGCGG ACACCGTGGA CTTCATCATC ATTGTCTTCG GCTTCTGGGC CTTCGGGAAA 
CACTCGGCTG CAGCCGACAT CACGTCCTCA CTGTCCGAGG ACCAGGTGCC AGGGCCCTTC 
CTGGTGATGG TGCTCATTCA GTTCGGGACT ATGGTGGTGG ACCGGGCGCT CTACCTCAGG 
AAGACTGTCC TGGGAAAGGT CATCTTCCAG GTCATCCTGG TGTTTGGGAT TCACTTCTGG 
ATGTTCTTCA TCCTGCCTGG CGTGACCGAG AGGAAGTTCA GCCAGAACCT GGTCGCGCAG 
CTGTGGTACT TCGTCAAGTG CGTGTACTTC GGGCTGTCCG CCTACCAGAT CCGCTGCGGC 
TACCCAACTC GGGTCCTCGG CAACTTCCTC ACCAAGAGTT ACAACTACGT CAACCTCTTC 
CTGTTCCAGG GGTTCCGCCT TGTCCCTTTC CTGACGGAGC TGAGGGCTGT GATGGACTGG 
GTGTGGACAG ACACGACCCT GAGCCTGTCC AGCTGGATCT GCGTGGAGGA CATCTATGCT 
CACATCTTCA TCCTCAAGTG TTGGCGGGAG TCGGAAAAAA GGTACCCGCA GCCCCGGGGG 
CAGAAGAAGA AGAAGGTGGT CAAGTACGGG ATGGGGGGCA TGATCATCGT GCTGCTCATC 
TGCATCGTCT GGTTCCCGCT ACTCTTCATG TCTCTGGTCA AATCCGTCGC TGGGGTTATC 
AACCAACCCC TGGACGTCTC AGTCACAATC ACCCTGGGCG GGTACCAGCC TATTTTCACA 
ATGAGTGCCC AGCAAAGCCA GCTGAAAGTT ATGGACCAGC CAATGTTTAA TAAATTCATT 
AAAGCTTTTT CTAAGGACAC TGGTGCTATG CAATTTCTGG AAAATTATGA AAAAGAAGAC 
ATAACAGTCG CAGAACTGGA AGGAAACTCA AATTCTTTAT GGACCATCAG CCCTCCCAGT 
AAGCAGAAAA TGATACACGA ACTCAAGGAC CCCAATAGTA GCTTCTCTGT TGTTTTTTCA 
TGGAGTATTC AGAGAAACAT GAGTCTGGGT GCAAAAGCAG AAATAGCAAC AGATAAGCTT 
TCTTTCCCTC TTAAAAACAA GACACGAGAG AATATTGCTA AAATGATAGC TGGCGGCGAC 
ACGGAAAGTT CCAGGACGCC CGTGACCATA GAAAGGATTT ACCCGTACTA TGTGAAAGCA 
CCCAGTGACT CTAACTCCAA ACCCATAAAG CAGCTTTTAT CTGAAAATAA TTTCATGAAC 
ATCACCATCA TTTTGTCCAG AGACAATGCG ACTAACTCGA ACAGTGAGTG GTGGGTTCTC 
AACTTGACTG GGAACAGAAT CTACAACCAG GAAGCGCAGG CCCTGGAGCT GGTGGTCTTC 
AACGACAAGG TCAGCCCCCC CAGCCTGGGG TTCCTGGCCG GCTACGGTAT CATGGGCCTC 
TACGCTTCCG TGGTGCTGGT GATTGGGAAG TTTGTCCGGG AATTCTTCAG CGGGATTTCT 
CACTCCATCA TGTTTGAAGA ACTTCCCAAT GTGGATCGGA TTCTGAAATT GTGCACAGAC 
ATTTTTTTAG TCCGGGAGAC CGGAGAGCTG GAGCTCGAGG AGGATCTCTA TGCCAAATTA 
ATATTCCTGT ACCGGTCGCC AGAAACCATG ATCAAATGGA CCAGGGAGAA AACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_012103589.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011958979.1 Ovis aries piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.

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CloneID	OSm26168
Clone ID Related Accession (Same CDS sequence)	XM_012103589.2
Accession Version	XM_012103589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	piezo-type mechanosensitive ion channel component 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639032.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012103589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTGG CACCCCAGTC CTGGAGACCC TTTGCTCGGA CTCGCGGGAC CGACGCGATG 
GCCTCGGAAG TGGTGTGCGG GCTCGTCTTC AGGCTGCTGC TCCCCATCTG CCTGGCAGTA 
GCCTGTGCCT TCCGGTACAA CGGGCTCTCC TTCGTCTACC TCATCTACCT CCTGCTCATC 
CCCCTGTTCT CGGAACCAAC TAAAGCCACC ATGCAAGGAC ACACGGGACG GTTGTTAAAG 
TCTCTGTGCT TCACCAGTCT CTCCTTCCTG TTGCTGCACA TCATTTTCCA CATCACGTTG 
GCGAGTCTGG AGGCACAGCA CCGCATTGCA CCTGGTTACA ACTGCTCAAC CTGGGAGAAG 
ACTTTCCGAC AAATCGGCTT CGAAAGCTTA AAGGGAGCCG ACGCTGGCAA TGGGATCCGA 
GTGTTTGTGC CTGACGTGGG CATGTTCATC GCCAGCCTGA CCATCTGGCT GGTCTGTAGG 
AACATTGTTC AGAAGCCAGT GGCCGAAGAG GCCGCGCAGT GTAACCCGGA GTTTGAGAAC 
GAAGAGTCGG CTGGAGGAGA AAAAATAGAC TCCGAAGAGG CCCTGATCTA TGAAGAGGAC 
TTAGATGAAG GAGACGGTGC TGAAGGCGAT TTGGAGGAAA GCACGAAATT AAAACTCTTC 
CGCAGGCTGG CCTCCGTGGC CTCCAAGCTG AAGGAGTTTA TTGGCAACAT GATAACTACA 
GCGGGGAAGG TTGTTGTGAC AATTTTACTG GGTTCATCAG GGATGATGCT GCCCTCTCTG 
ACCTCATCCG TGTACTTCTT CGTGTTCCTG GGCCTGTGTA CCTGGTGGTC CTGGTGCCGG 
ACCTTCGACC CCTTGCTGTT CAGCTGTCTC TGCGTCCTGC TGGCCATCTT CACTGCGGGA 
CACCTGATCG GACTCTATCT GTACCAGTTC CAGTTCTTCC AGGAAGCCGT TCCCCCCAAC 
GACTACTATG CCAGGTTGTT TGGTATCAAA TCAGTCATTC AGACGGACTG TTCCAGCACA 
TGGAAGATCA TTGCCAACCC CGACCTGTCC TGGTACCACC ACGCCAACCC CATCGTCCTG 
CTGGTGATGT ACTACACGCT GGCTACTCTG ATCCGAATCT GGCTGCAGGA GCCCGTTGTG 
CAGGAAGAGA AGACCAAGGA GGAGGACAGA GCCCTGGCCT GCAGCCCCGC GCCCATCACG 
GCCGAGAGGA GGCGGAGCCT GTGGTACGCG ACCCACTACC CCACCGACGA GAGAAAACTT 
TTATCCATGA CTCAGGATGA CTACAAGCCG TCCGACGGCC TGCTGGTGAC CGTGAATGGC 
AACCCCGTGG ACTACCACAC CATCCACCCC AGCCTCCCAC TGGAGAACGG CCCCGCCAAG 
GCTGACCTGT ACTCCACACC ACAGTACCGC TGGGAGCCTG CCACTGCCTC CCCTGAAAAG 
AAGGAGGAAG ATAATGATGA GAAAGAAGAG TTTGAGGAGG AGCGGAGCCG GGAGGAGAAG 
AGGAGTGTCA AAGTGCACGC CATGGTGTCT GTGTTCCAGT TTATCATGAA ACAGAGCTAC 
ATCTGTGCCC TCATCGCCAT GATGGCCTGG AGCATCACCT ACCACAGCTG GCTGACCTTC 
GTGCTGCTCA TCTGGTCCTG CACCCTGTGG ATGATCCGCA ACCGGAGGAA GTATGCCATG 
ATCAGCTCCC CCTTCATGGT CGTTTACGGA AACCTGCTGC TCATACTGCA GTACATATGG 
AGCTTCGAGC TCCCTGAGAT TAAAAAGGTC CCGGGTTTCT TAGAAAAGAA GGAGCCGGGG 
GAGCTCGCCT CAAAGATTCT GTTCACCATC ACATTCTGGC TGCTGCTGAG ACAGCACCTC 
ACAGAGCAGA AAGCTCTCCA AGAAAAGGAA GCTCTTCTGT CTGAGGTCAA AATCGGAAGT 
CAGGAAAATG AAGAAAAAGA TGATGAACAG GATGTACAGG TAGACAGCGA GGCTCAGGAG 
GAGGAAGGAG AGGAGCCGAA GGAGGAGCAG CAGGAGCGGA GAGAGGAAGA GGAGGAGGAA 
GAGGCAGACG AGCAGGACGT GATGAAGGTG CTGGGGAACC TGGTGGTGGC CCTGTTCATC 
AAGTACTGGA TCTACGTCTG CGGGGGGATG TTCTTCTTCG TCAGCTTCGA GGGTAAAATC 
GTGATGTACA AGATCATCTA CATGGTACTC TTCCTCTTCT GTGTGGCCCT GTATCAGGTG 
CACTATGAAT GGTGGAGGAG GATTCTGAAG TACTTCTGGA TGTCGGTGGT TATCTACACA 
ATGCTGGTGC TTATCTTTAT ATACACATAT CAGTTTGAAA ACTTCCCAGG CTTGTGGCAA 
AACATGACTG GACTGAAAAA AGAAAAGCTG GAGGACCTTG GCCTGAAGCA GTTCACGGTG 
GCCGAGCTGT TCACGCGCAT CTTCATCCCC ACCTCCTTCC TCCTGGTCTG CATTCTGCAC 
CTGCACTACT TCCACGACCG ATTCCTGGAG CTCACCGACC TGAAGTCCAT CCCCAGCAAG 
GAGGACAGCG CTGTCTATAG GCTGGCCCAC CCCGAGGGAA GCCTTCCAGA CCTTGCCATG 
ATGAAACTGA CTGCCAGCCT GGAGGTGGCA GAGGTCAAGC AGCTGGCTGG GCCTGGGGAG 
GAGATGCCAG GGGGATGCCC TGCAAAAGCC AAGAAGGGCG AGCCTGGCAG GGACAGCGAG 
GACGAGGATG AGGATGAGGA CGAGGAGGAT GAAGAGGAGG AGGAGACGTC AGATCTGAGG 
AACAAGTGGC ACCTGGTGAT CGACCGCCTC ACAGTGCTGC TGCTGCGATT CCTGGAGGGC 
TTCCACCAGC TGCAGACATT CCTGTGGTGG ATCCTGGAGC TGCACATCGT CAAGATTGTG 
TCCTCCTACA TCATCTGGGT GTCCGTGAAG GAGGTGTCTC TGTTCAACTA CGTGTTTCTG 
ATTTCCTGGG CTTTTGCTCT GCCGTATGCT AAGCTCCGCC GTCTGGCCTC CAGTGTCTGC 
ACCGTCTGGA CATGTGTGAT CATTGTCTGT AAGATGCTGT ACCAGCTGCA GACCATCAAG 
CCTGAGAACT TCTCTGTGAA CTGTTCGTTG CCAAATGAAA ACCAGACGAA CATCCCCCTG 
CACCAGCTGA ACAAGTCTCT GCTCTACAGC GCACCCATTG ACCCCACAGA ATGGGTGGGC 
CTCAGGAAGT CGTCTCCTCT ACTTGTCTAC CTGAGGAACA ACCTGCTGAT GCTGGCCATC 
CTGGCCTTCG AGGTCACCAT CTACCGCCAT CAGGAGTACT ACCGTGGTCG GAACAACCTC 
TCAACCCCCG TGTCTAAAAC CATCTTCCAC AACATCACAA GACTCCAGCT GGATGATGGA 
CTTGTTAACT GTGCCAAGTA TTTCATCAAT TACTTCTTCT ACAAGTTTGG TCTGGAGACC 
TGTTTCCTCA TGTCAGTTAA TGTGATTGGC CAGCGCATGG ACTTCTACGC CATGATTCAC 
GCCTGCTGGC TGATTGCCGT CCTGTACCGG CGCAGGAGGA AGGCCATCGC CGAGATCTGG 
CCCAAGTACT GCTGCTTCCT GGCTTGCATC ATCACCTTCC AGTATTTCAT CTGCATCGGC 
ATCCCCCCTG CCCCCTGTCG AGACTACCCT TGGAGGTTCA AGGGCGCCAG CTTCAATGAC 
AACATCGTCA AGTGGCTGTA CTTCCCTGAC TTCATCGTTC GACCCAACCC TGTGTTCCTC 
GTGTATGATT TCATGCTACT CCTGTGTGCC TCGCTGCAAC GGCAGATCTT TGAGGATGAG 
AATAAGGCTG CTGTACGGAT CGTGGCGGGC GACAACGTGG AAATCTGCAT GAACCTGGAT 
GCGGCCTCAT TTAGCCAGCA CAATCCCGTG CCCGACTTCA TCCACTGCAG GTCGTACCTG 
GACATGTCCA AGGTGGTCAT CTTCAGCTAC CTCTTCTGGT TCGTGCTCAC TATCATCTTC 
ATCACCGGGA CGACTCGCAT CAGCATCTTT TGCATGGGCT ACCTGGTGGC CTGCTTCTAC 
TTCCTGCTCT TCGGGGGCGA CTTGCTGCTG AAACCCATCA AGAGCATCCT GCGCTACTGG 
GACTGGCTGA TCGCTTACAA TGTCTTCGTG ATCACGATGA AAAACATACT GTCGATAGGA 
GCGTGTGGCT ATATTGAAAA ATTGGTTCAA AATAGTTGCT GGTTGATCCA AGCATTCAGC 
CTGGCCTGTA CAGTCAAGGG CTACAAAATG CCTGATGATG ACTCCTCCTG TAAACTGCCC 
AGTGGGGAGG CAGGAATCAT TTGGGACAGC ATATGCTTTG CCTTCCTGCT GCTGCAGAGA 
AGAGTGTTCA TGAGTTACTA TTTCCTGCAT GTGGTGGCAG ATATAAAAGC CTCACAGATC 
CTGGCATCCA GAGGGGCCGA GCTTTTCCAG GCCACCATTG TGAAGGCTGT GAAGGCCAGA 
ATCGAGGAGG AGAAAAAGTC CATGGACCAG CTGAAGAGGC AGATGGACCG CATCAAGGCC 
AGGCAACAGA AATACAAAAA GGGTAAGGAG AGGATGCTGA GCTTGACCCA GGAGTCGGGG 
GAAGGCCAAG ATCTCCAAAA ACTTTCTGAA GAGGATGACG AGAGAGAAGC AGACAAACAG 
AAAACCAAGG GCAAAAAAAA GCAGTGGTGG CGGCCTTGGG TTGATCATGT TTCCATGGTC 
AGGAGTGGAG ATTACTATTT GTTTGAAACG GATAGTGAAG AGGAGGAAGA GGAAGAATTA 
AAAAAGGAAG ACGAAGAGCC CCCCCGGAGG TCAGCCTTCC AGTTTGTTTA TCAAGCCTGG 
ATTACTGACC CTAAAACAGC ACTCCGACAG AGGCGCAAAG AGAAAAAAAG ATCTGCAAGA 
GAAGAACAGA AAAGAAGGAG AAAAGTATCT GGGGAGGGTC CTGTGGAGTG GGAAGACCGG 
GAAGATGAGC CAGTCAAAAA GAAGTCTGAT GGACCAGACA ACATCATCAA GAGGATCTTT 
AATATTTTGA AGTTCACCTG GGTCCTGTTT CTGGCAACCG TGGACAGTTT CACAACGTGG 
CTCAACTCCA TCTCCAGAGA GCACATCGAC ATCTCCACTG TCCTGAGGAT CGAGCGCTGC 
ATGCTGACCA GAGAGATCAA GAAGGGCAAC GTCCCAACCC GGGAGAGCAT CCACATGTAC 
TACCAGAACC ATATCATGAA CCTGTCCAGG GAGTCGGGGC TGGACACCAT CGACGACCAT 
GCTGGCGCTG CTGCGGGCGC ACAGACCGCC CACAGGATGG ACAGCTTGGA TTCCCACGAC 
AGCATCTCCA GCGAGCCCAC GCAGTGCACG ATGCTGTACT CGCGCCAGGG GACCACGGAG 
ACCATCGAGG AAGTGGAGGC TGAGCAGGAC GAGGAGACAT CCGAGGGGGC CCGGCAGGCC 
GAGTCCCCAC AGGACCCCAC GGAGGATGGT GACGTGGAGG CCCCGCCGTC CTACAGCAAG 
GCTGTCAGCT TTGAACGCCT GTCCTTCGAC TCCCGGGATG ACTCAGCTGG CCAGACCCAC 
ATGGAAGTCA GCCCGGATGA CAGCCGCTCA GATCGGCTGG AGGCCAGCAT CCTGCCACCC 
CTGACCCACG AGCTGACAGC CAGCGAGCTG CTGCTGAACA AGATGTTCCA TGACGACGAG 
CTGGAGGAGT CAGAGAAGTT CTACGTGGGG CAGCCTCGCT TCCTGCTGCT CTTCTACGCG 
CTGTACAACA CGCTGGTGGC CCGCTCCGAG ATGGTGTGCT ACTTTGTGAT CATCCTCAAC 
CACATGGTCT CCGCCTCCAT GATCACCCTC GTGCTGCCCA TCCTCATCTT CCTCTGGGCC 
ATGCTGTCCG TCCCCCGGCC CAGCAGGCGC TTCTGGATGA TGGCCATCGT CTACACGGAG 
GTGGCCATTG TGGTCAAGTA CTTCTTCCAA TTTGGGTTCT TCCCCTGGAA CAAGAATGTG 
GAACTGAACA AGGACAAGCC TTACCACCCA CCCAACATCA TCGGGGTGGA GAAGAAGGAC 
GCCTACGTGC TCTACGACCT CATCCAGCTG CTCGCACTCT TCTTCCACCG CTCCATCCTA 
AAGTGTCACG GCCTCTGGGA TGAAGACGAC ATCGTGGACA GCGGCTCGGA CAAGGTGGAC 
TCGGACGACG AGCTGTCCCT CGGCCATGGT AGGAGGGCCT CCTCCGACTC GCTCAAGTCC 
ATCAACCTGG CTGCATCCGT GGAGTCGGTA CACGTGACCT TCCCAGAGCA GCCGGCTGCC 
GTCCGGAGGA AGCGCTCCAG CAGCGGCTCC CAGACATCAC AGGAGTCCAG CTTTTCCTCG 
AATAGGTCCA AGAGAGGCAG CACGAGCACC CGGAACAGCA GCCAGAAAGG AAGCAGCGTC 
GTGAGCGTCA AGCAGAAGAG CAGACGGGAA CTTTACATGG AGAAGCTGCA GGAACACTTA 
ATCAAAGCAA AGGCCTTTAC CATAAAGAAG ACCCTGCAGC TCTACGTGCC CATCAGGCAG 
TTCTTCTACG ACCTCATCCA CCCCGACTAC AGCGCCGTCA CCGACGTGTA CGTGCTTATG 
TTCCTGGCGG ACACCGTGGA CTTCATCATC ATTGTCTTCG GCTTCTGGGC CTTCGGGAAA 
CACTCGGCTG CAGCCGACAT CACGTCCTCA CTGTCCGAGG ACCAGGTGCC AGGGCCCTTC 
CTGGTGATGG TGCTCATTCA GTTCGGGACT ATGGTGGTGG ACCGGGCGCT CTACCTCAGG 
AAGACTGTCC TGGGAAAGGT CATCTTCCAG GTCATCCTGG TGTTTGGGAT TCACTTCTGG 
ATGTTCTTCA TCCTGCCTGG CGTGACCGAG AGGAAGTTCA GCCAGAACCT GGTCGCGCAG 
CTGTGGTACT TCGTCAAGTG CGTGTACTTC GGGCTGTCCG CCTACCAGAT CCGCTGCGGC 
TACCCAACTC GGGTCCTCGG CAACTTCCTC ACCAAGAGTT ACAACTACGT CAACCTCTTC 
CTGTTCCAGG GGTTCCGCCT TGTCCCTTTC CTGACGGAGC TGAGGGCTGT GATGGACTGG 
GTGTGGACAG ACACGACCCT GAGCCTGTCC AGCTGGATCT GCGTGGAGGA CATCTATGCT 
CACATCTTCA TCCTCAAGTG TTGGCGGGAG TCGGAAAAAA GGTACCCGCA GCCCCGGGGG 
CAGAAGAAGA AGAAGGTGGT CAAGTACGGG ATGGGGGGCA TGATCATCGT GCTGCTCATC 
TGCATCGTCT GGTTCCCGCT ACTCTTCATG TCTCTGGTCA AATCCGTCGC TGGGGTTATC 
AACCAACCCC TGGACGTCTC AGTCACAATC ACCCTGGGCG GGTACCAGCC TATTTTCACA 
ATGAGTGCCC AGCAAAGCCA GCTGAAAGTT ATGGACCAGC CAATGTTTAA TAAATTCATT 
AAAGCTTTTT CTAAGGACAC TGGTGCTATG CAATTTCTGG AAAATTATGA AAAAGAAGAC 
ATAACAGTCG CAGAACTGGA AGGAAACTCA AATTCTTTAT GGACCATCAG CCCTCCCAGT 
AAGCAGAAAA TGATACACGA ACTCAAGGAC CCCAATAGTA GCTTCTCTGT TGTTTTTTCA 
TGGAGTATTC AGAGAAACAT GAGTCTGGGT GCAAAAGCAG AAATAGCAAC AGATAAGCTT 
TCTTTCCCTC TTAAAAACAA GACACGAGAG AATATTGCTA AAATGATAGC TGGCGGCGAC 
ACGGAAAGTT CCAGGACGCC CGTGACCATA GAAAGGATTT ACCCGTACTA TGTGAAAGCA 
CCCAGTGACT CTAACTCCAA ACCCATAAAG CAGCTTTTAT CTGAAAATAA TTTCATGAAC 
ATCACCATCA TTTTGTCCAG AGACAATGCG ACTAACTCGA ACAGTGAGTG GTGGGTTCTC 
AACTTGACTG GGAACAGAAT CTACAACCAG GAAGCGCAGG CCCTGGAGCT GGTGGTCTTC 
AACGACAAGG TCAGCCCCCC CAGCCTGGGG TTCCTGGCCG GCTACGGTAT CATGGGCCTC 
TACGCTTCCG TGGTGCTGGT GATTGGGAAG TTTGTCCGGG AATTCTTCAG CGGGATTTCT 
CACTCCATCA TGTTTGAAGA ACTTCCCAAT GTGGATCGGA TTCTGAAATT GTGCACAGAC 
ATTTTTTTAG TCCGGGAGAC CGGAGAGCTG GAGCTCGAGG AGGATCTCTA TGCCAAATTA 
ATATTCCTGT ACCGGTCGCC AGAAACCATG ATCAAATGGA CCAGGGAGAA AACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011958979.1
CDS	747..8966
Translation	MGLAPQSWRPFARTRGTDAMASEVVCGLVFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKATMQGHTGRLLKSLCFTSLSFLLLHIIFHITLASLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDVGMFIASLTIWLVCRNIVQKPVAEEAAQCNPEFENEESAGGEKIDSEEALIYEEDLDEGDGAEGDLEESTKLKLFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIANPDLSWYHHANPIVLLVMYYTLATLIRIWLQEPVVQEEKTKEEDRALACSPAPITAERRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPLENGPAKADLYSTPQYRWEPATASPEKKEEDNDEKEEFEEERSREEKRSVKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDDEQDVQVDSEAQEEEGEEPKEEQQERREEEEEEEADEQDVMKVLGNLVVALFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRRILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDSAVYRLAHPEGSLPDLAMMKLTASLEVAEVKQLAGPGEEMPGGCPAKAKKGEPGRDSEDEDEDEDEEDEEEEETSDLRNKWHLVIDRLTVLLLRFLEGFHQLQTFLWWILELHIVKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPLHQLNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLSTPVSKTIFHNITRLQLDDGLVNCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIVKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIVAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVVIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIEKLVQNSCWLIQAFSLACTVKGYKMPDDDSSCKLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQESGEGQDLQKLSEEDDEREADKQKTKGKKKQWWRPWVDHVSMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRRKEKKRSAREEQKRRRKVSGEGPVEWEDREDEPVKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDDHAGAAAGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQDEETSEGARQAESPQDPTEDGDVEAPPSYSKAVSFERLSFDSRDDSAGQTHMEVSPDDSRSDRLEASILPPLTHELTASELLLNKMFHDDELEESEKFYVGQPRFLLLFYALYNTLVARSEMVCYFVIILNHMVSASMITLVLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVELNKDKPYHPPNIIGVEKKDAYVLYDLIQLLALFFHRSILKCHGLWDEDDIVDSGSDKVDSDDELSLGHGRRASSDSLKSINLAASVESVHVTFPEQPAAVRRKRSSSGSQTSQESSFSSNRSKRGSTSTRNSSQKGSSVVSVKQKSRRELYMEKLQEHLIKAKAFTIKKTLQLYVPIRQFFYDLIHPDYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLVKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQPMFNKFIKAFSKDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELKDPNSSFSVVFSWSIQRNMSLGAKAEIATDKLSFPLKNKTRENIAKMIAGGDTESSRTPVTIERIYPYYVKAPSDSNSKPIKQLLSENNFMNITIILSRDNATNSNSEWWVLNLTGNRIYNQEAQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_012103589.2
Organism	Ovis aries(sheep)
Definition	Ovis aries piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012103589.2

ORF Insert Sequence:

ATGGGCTTGG CACCCCAGTC CTGGAGACCC TTTGCTCGGA CTCGCGGGAC CGACGCGATG 
GCCTCGGAAG TGGTGTGCGG GCTCGTCTTC AGGCTGCTGC TCCCCATCTG CCTGGCAGTA 
GCCTGTGCCT TCCGGTACAA CGGGCTCTCC TTCGTCTACC TCATCTACCT CCTGCTCATC 
CCCCTGTTCT CGGAACCAAC TAAAGCCACC ATGCAAGGAC ACACGGGACG GTTGTTAAAG 
TCTCTGTGCT TCACCAGTCT CTCCTTCCTG TTGCTGCACA TCATTTTCCA CATCACGTTG 
GCGAGTCTGG AGGCACAGCA CCGCATTGCA CCTGGTTACA ACTGCTCAAC CTGGGAGAAG 
ACTTTCCGAC AAATCGGCTT CGAAAGCTTA AAGGGAGCCG ACGCTGGCAA TGGGATCCGA 
GTGTTTGTGC CTGACGTGGG CATGTTCATC GCCAGCCTGA CCATCTGGCT GGTCTGTAGG 
AACATTGTTC AGAAGCCAGT GGCCGAAGAG GCCGCGCAGT GTAACCCGGA GTTTGAGAAC 
GAAGAGTCGG CTGGAGGAGA AAAAATAGAC TCCGAAGAGG CCCTGATCTA TGAAGAGGAC 
TTAGATGAAG GAGACGGTGC TGAAGGCGAT TTGGAGGAAA GCACGAAATT AAAACTCTTC 
CGCAGGCTGG CCTCCGTGGC CTCCAAGCTG AAGGAGTTTA TTGGCAACAT GATAACTACA 
GCGGGGAAGG TTGTTGTGAC AATTTTACTG GGTTCATCAG GGATGATGCT GCCCTCTCTG 
ACCTCATCCG TGTACTTCTT CGTGTTCCTG GGCCTGTGTA CCTGGTGGTC CTGGTGCCGG 
ACCTTCGACC CCTTGCTGTT CAGCTGTCTC TGCGTCCTGC TGGCCATCTT CACTGCGGGA 
CACCTGATCG GACTCTATCT GTACCAGTTC CAGTTCTTCC AGGAAGCCGT TCCCCCCAAC 
GACTACTATG CCAGGTTGTT TGGTATCAAA TCAGTCATTC AGACGGACTG TTCCAGCACA 
TGGAAGATCA TTGCCAACCC CGACCTGTCC TGGTACCACC ACGCCAACCC CATCGTCCTG 
CTGGTGATGT ACTACACGCT GGCTACTCTG ATCCGAATCT GGCTGCAGGA GCCCGTTGTG 
CAGGAAGAGA AGACCAAGGA GGAGGACAGA GCCCTGGCCT GCAGCCCCGC GCCCATCACG 
GCCGAGAGGA GGCGGAGCCT GTGGTACGCG ACCCACTACC CCACCGACGA GAGAAAACTT 
TTATCCATGA CTCAGGATGA CTACAAGCCG TCCGACGGCC TGCTGGTGAC CGTGAATGGC 
AACCCCGTGG ACTACCACAC CATCCACCCC AGCCTCCCAC TGGAGAACGG CCCCGCCAAG 
GCTGACCTGT ACTCCACACC ACAGTACCGC TGGGAGCCTG CCACTGCCTC CCCTGAAAAG 
AAGGAGGAAG ATAATGATGA GAAAGAAGAG TTTGAGGAGG AGCGGAGCCG GGAGGAGAAG 
AGGAGTGTCA AAGTGCACGC CATGGTGTCT GTGTTCCAGT TTATCATGAA ACAGAGCTAC 
ATCTGTGCCC TCATCGCCAT GATGGCCTGG AGCATCACCT ACCACAGCTG GCTGACCTTC 
GTGCTGCTCA TCTGGTCCTG CACCCTGTGG ATGATCCGCA ACCGGAGGAA GTATGCCATG 
ATCAGCTCCC CCTTCATGGT CGTTTACGGA AACCTGCTGC TCATACTGCA GTACATATGG 
AGCTTCGAGC TCCCTGAGAT TAAAAAGGTC CCGGGTTTCT TAGAAAAGAA GGAGCCGGGG 
GAGCTCGCCT CAAAGATTCT GTTCACCATC ACATTCTGGC TGCTGCTGAG ACAGCACCTC 
ACAGAGCAGA AAGCTCTCCA AGAAAAGGAA GCTCTTCTGT CTGAGGTCAA AATCGGAAGT 
CAGGAAAATG AAGAAAAAGA TGATGAACAG GATGTACAGG TAGACAGCGA GGCTCAGGAG 
GAGGAAGGAG AGGAGCCGAA GGAGGAGCAG CAGGAGCGGA GAGAGGAAGA GGAGGAGGAA 
GAGGCAGACG AGCAGGACGT GATGAAGGTG CTGGGGAACC TGGTGGTGGC CCTGTTCATC 
AAGTACTGGA TCTACGTCTG CGGGGGGATG TTCTTCTTCG TCAGCTTCGA GGGTAAAATC 
GTGATGTACA AGATCATCTA CATGGTACTC TTCCTCTTCT GTGTGGCCCT GTATCAGGTG 
CACTATGAAT GGTGGAGGAG GATTCTGAAG TACTTCTGGA TGTCGGTGGT TATCTACACA 
ATGCTGGTGC TTATCTTTAT ATACACATAT CAGTTTGAAA ACTTCCCAGG CTTGTGGCAA 
AACATGACTG GACTGAAAAA AGAAAAGCTG GAGGACCTTG GCCTGAAGCA GTTCACGGTG 
GCCGAGCTGT TCACGCGCAT CTTCATCCCC ACCTCCTTCC TCCTGGTCTG CATTCTGCAC 
CTGCACTACT TCCACGACCG ATTCCTGGAG CTCACCGACC TGAAGTCCAT CCCCAGCAAG 
GAGGACAGCG CTGTCTATAG GCTGGCCCAC CCCGAGGGAA GCCTTCCAGA CCTTGCCATG 
ATGAAACTGA CTGCCAGCCT GGAGGTGGCA GAGGTCAAGC AGCTGGCTGG GCCTGGGGAG 
GAGATGCCAG GGGGATGCCC TGCAAAAGCC AAGAAGGGCG AGCCTGGCAG GGACAGCGAG 
GACGAGGATG AGGATGAGGA CGAGGAGGAT GAAGAGGAGG AGGAGACGTC AGATCTGAGG 
AACAAGTGGC ACCTGGTGAT CGACCGCCTC ACAGTGCTGC TGCTGCGATT CCTGGAGGGC 
TTCCACCAGC TGCAGACATT CCTGTGGTGG ATCCTGGAGC TGCACATCGT CAAGATTGTG 
TCCTCCTACA TCATCTGGGT GTCCGTGAAG GAGGTGTCTC TGTTCAACTA CGTGTTTCTG 
ATTTCCTGGG CTTTTGCTCT GCCGTATGCT AAGCTCCGCC GTCTGGCCTC CAGTGTCTGC 
ACCGTCTGGA CATGTGTGAT CATTGTCTGT AAGATGCTGT ACCAGCTGCA GACCATCAAG 
CCTGAGAACT TCTCTGTGAA CTGTTCGTTG CCAAATGAAA ACCAGACGAA CATCCCCCTG 
CACCAGCTGA ACAAGTCTCT GCTCTACAGC GCACCCATTG ACCCCACAGA ATGGGTGGGC 
CTCAGGAAGT CGTCTCCTCT ACTTGTCTAC CTGAGGAACA ACCTGCTGAT GCTGGCCATC 
CTGGCCTTCG AGGTCACCAT CTACCGCCAT CAGGAGTACT ACCGTGGTCG GAACAACCTC 
TCAACCCCCG TGTCTAAAAC CATCTTCCAC AACATCACAA GACTCCAGCT GGATGATGGA 
CTTGTTAACT GTGCCAAGTA TTTCATCAAT TACTTCTTCT ACAAGTTTGG TCTGGAGACC 
TGTTTCCTCA TGTCAGTTAA TGTGATTGGC CAGCGCATGG ACTTCTACGC CATGATTCAC 
GCCTGCTGGC TGATTGCCGT CCTGTACCGG CGCAGGAGGA AGGCCATCGC CGAGATCTGG 
CCCAAGTACT GCTGCTTCCT GGCTTGCATC ATCACCTTCC AGTATTTCAT CTGCATCGGC 
ATCCCCCCTG CCCCCTGTCG AGACTACCCT TGGAGGTTCA AGGGCGCCAG CTTCAATGAC 
AACATCGTCA AGTGGCTGTA CTTCCCTGAC TTCATCGTTC GACCCAACCC TGTGTTCCTC 
GTGTATGATT TCATGCTACT CCTGTGTGCC TCGCTGCAAC GGCAGATCTT TGAGGATGAG 
AATAAGGCTG CTGTACGGAT CGTGGCGGGC GACAACGTGG AAATCTGCAT GAACCTGGAT 
GCGGCCTCAT TTAGCCAGCA CAATCCCGTG CCCGACTTCA TCCACTGCAG GTCGTACCTG 
GACATGTCCA AGGTGGTCAT CTTCAGCTAC CTCTTCTGGT TCGTGCTCAC TATCATCTTC 
ATCACCGGGA CGACTCGCAT CAGCATCTTT TGCATGGGCT ACCTGGTGGC CTGCTTCTAC 
TTCCTGCTCT TCGGGGGCGA CTTGCTGCTG AAACCCATCA AGAGCATCCT GCGCTACTGG 
GACTGGCTGA TCGCTTACAA TGTCTTCGTG ATCACGATGA AAAACATACT GTCGATAGGA 
GCGTGTGGCT ATATTGAAAA ATTGGTTCAA AATAGTTGCT GGTTGATCCA AGCATTCAGC 
CTGGCCTGTA CAGTCAAGGG CTACAAAATG CCTGATGATG ACTCCTCCTG TAAACTGCCC 
AGTGGGGAGG CAGGAATCAT TTGGGACAGC ATATGCTTTG CCTTCCTGCT GCTGCAGAGA 
AGAGTGTTCA TGAGTTACTA TTTCCTGCAT GTGGTGGCAG ATATAAAAGC CTCACAGATC 
CTGGCATCCA GAGGGGCCGA GCTTTTCCAG GCCACCATTG TGAAGGCTGT GAAGGCCAGA 
ATCGAGGAGG AGAAAAAGTC CATGGACCAG CTGAAGAGGC AGATGGACCG CATCAAGGCC 
AGGCAACAGA AATACAAAAA GGGTAAGGAG AGGATGCTGA GCTTGACCCA GGAGTCGGGG 
GAAGGCCAAG ATCTCCAAAA ACTTTCTGAA GAGGATGACG AGAGAGAAGC AGACAAACAG 
AAAACCAAGG GCAAAAAAAA GCAGTGGTGG CGGCCTTGGG TTGATCATGT TTCCATGGTC 
AGGAGTGGAG ATTACTATTT GTTTGAAACG GATAGTGAAG AGGAGGAAGA GGAAGAATTA 
AAAAAGGAAG ACGAAGAGCC CCCCCGGAGG TCAGCCTTCC AGTTTGTTTA TCAAGCCTGG 
ATTACTGACC CTAAAACAGC ACTCCGACAG AGGCGCAAAG AGAAAAAAAG ATCTGCAAGA 
GAAGAACAGA AAAGAAGGAG AAAAGTATCT GGGGAGGGTC CTGTGGAGTG GGAAGACCGG 
GAAGATGAGC CAGTCAAAAA GAAGTCTGAT GGACCAGACA ACATCATCAA GAGGATCTTT 
AATATTTTGA AGTTCACCTG GGTCCTGTTT CTGGCAACCG TGGACAGTTT CACAACGTGG 
CTCAACTCCA TCTCCAGAGA GCACATCGAC ATCTCCACTG TCCTGAGGAT CGAGCGCTGC 
ATGCTGACCA GAGAGATCAA GAAGGGCAAC GTCCCAACCC GGGAGAGCAT CCACATGTAC 
TACCAGAACC ATATCATGAA CCTGTCCAGG GAGTCGGGGC TGGACACCAT CGACGACCAT 
GCTGGCGCTG CTGCGGGCGC ACAGACCGCC CACAGGATGG ACAGCTTGGA TTCCCACGAC 
AGCATCTCCA GCGAGCCCAC GCAGTGCACG ATGCTGTACT CGCGCCAGGG GACCACGGAG 
ACCATCGAGG AAGTGGAGGC TGAGCAGGAC GAGGAGACAT CCGAGGGGGC CCGGCAGGCC 
GAGTCCCCAC AGGACCCCAC GGAGGATGGT GACGTGGAGG CCCCGCCGTC CTACAGCAAG 
GCTGTCAGCT TTGAACGCCT GTCCTTCGAC TCCCGGGATG ACTCAGCTGG CCAGACCCAC 
ATGGAAGTCA GCCCGGATGA CAGCCGCTCA GATCGGCTGG AGGCCAGCAT CCTGCCACCC 
CTGACCCACG AGCTGACAGC CAGCGAGCTG CTGCTGAACA AGATGTTCCA TGACGACGAG 
CTGGAGGAGT CAGAGAAGTT CTACGTGGGG CAGCCTCGCT TCCTGCTGCT CTTCTACGCG 
CTGTACAACA CGCTGGTGGC CCGCTCCGAG ATGGTGTGCT ACTTTGTGAT CATCCTCAAC 
CACATGGTCT CCGCCTCCAT GATCACCCTC GTGCTGCCCA TCCTCATCTT CCTCTGGGCC 
ATGCTGTCCG TCCCCCGGCC CAGCAGGCGC TTCTGGATGA TGGCCATCGT CTACACGGAG 
GTGGCCATTG TGGTCAAGTA CTTCTTCCAA TTTGGGTTCT TCCCCTGGAA CAAGAATGTG 
GAACTGAACA AGGACAAGCC TTACCACCCA CCCAACATCA TCGGGGTGGA GAAGAAGGAC 
GCCTACGTGC TCTACGACCT CATCCAGCTG CTCGCACTCT TCTTCCACCG CTCCATCCTA 
AAGTGTCACG GCCTCTGGGA TGAAGACGAC ATCGTGGACA GCGGCTCGGA CAAGGTGGAC 
TCGGACGACG AGCTGTCCCT CGGCCATGGT AGGAGGGCCT CCTCCGACTC GCTCAAGTCC 
ATCAACCTGG CTGCATCCGT GGAGTCGGTA CACGTGACCT TCCCAGAGCA GCCGGCTGCC 
GTCCGGAGGA AGCGCTCCAG CAGCGGCTCC CAGACATCAC AGGAGTCCAG CTTTTCCTCG 
AATAGGTCCA AGAGAGGCAG CACGAGCACC CGGAACAGCA GCCAGAAAGG AAGCAGCGTC 
GTGAGCGTCA AGCAGAAGAG CAGACGGGAA CTTTACATGG AGAAGCTGCA GGAACACTTA 
ATCAAAGCAA AGGCCTTTAC CATAAAGAAG ACCCTGCAGC TCTACGTGCC CATCAGGCAG 
TTCTTCTACG ACCTCATCCA CCCCGACTAC AGCGCCGTCA CCGACGTGTA CGTGCTTATG 
TTCCTGGCGG ACACCGTGGA CTTCATCATC ATTGTCTTCG GCTTCTGGGC CTTCGGGAAA 
CACTCGGCTG CAGCCGACAT CACGTCCTCA CTGTCCGAGG ACCAGGTGCC AGGGCCCTTC 
CTGGTGATGG TGCTCATTCA GTTCGGGACT ATGGTGGTGG ACCGGGCGCT CTACCTCAGG 
AAGACTGTCC TGGGAAAGGT CATCTTCCAG GTCATCCTGG TGTTTGGGAT TCACTTCTGG 
ATGTTCTTCA TCCTGCCTGG CGTGACCGAG AGGAAGTTCA GCCAGAACCT GGTCGCGCAG 
CTGTGGTACT TCGTCAAGTG CGTGTACTTC GGGCTGTCCG CCTACCAGAT CCGCTGCGGC 
TACCCAACTC GGGTCCTCGG CAACTTCCTC ACCAAGAGTT ACAACTACGT CAACCTCTTC 
CTGTTCCAGG GGTTCCGCCT TGTCCCTTTC CTGACGGAGC TGAGGGCTGT GATGGACTGG 
GTGTGGACAG ACACGACCCT GAGCCTGTCC AGCTGGATCT GCGTGGAGGA CATCTATGCT 
CACATCTTCA TCCTCAAGTG TTGGCGGGAG TCGGAAAAAA GGTACCCGCA GCCCCGGGGG 
CAGAAGAAGA AGAAGGTGGT CAAGTACGGG ATGGGGGGCA TGATCATCGT GCTGCTCATC 
TGCATCGTCT GGTTCCCGCT ACTCTTCATG TCTCTGGTCA AATCCGTCGC TGGGGTTATC 
AACCAACCCC TGGACGTCTC AGTCACAATC ACCCTGGGCG GGTACCAGCC TATTTTCACA 
ATGAGTGCCC AGCAAAGCCA GCTGAAAGTT ATGGACCAGC CAATGTTTAA TAAATTCATT 
AAAGCTTTTT CTAAGGACAC TGGTGCTATG CAATTTCTGG AAAATTATGA AAAAGAAGAC 
ATAACAGTCG CAGAACTGGA AGGAAACTCA AATTCTTTAT GGACCATCAG CCCTCCCAGT 
AAGCAGAAAA TGATACACGA ACTCAAGGAC CCCAATAGTA GCTTCTCTGT TGTTTTTTCA 
TGGAGTATTC AGAGAAACAT GAGTCTGGGT GCAAAAGCAG AAATAGCAAC AGATAAGCTT 
TCTTTCCCTC TTAAAAACAA GACACGAGAG AATATTGCTA AAATGATAGC TGGCGGCGAC 
ACGGAAAGTT CCAGGACGCC CGTGACCATA GAAAGGATTT ACCCGTACTA TGTGAAAGCA 
CCCAGTGACT CTAACTCCAA ACCCATAAAG CAGCTTTTAT CTGAAAATAA TTTCATGAAC 
ATCACCATCA TTTTGTCCAG AGACAATGCG ACTAACTCGA ACAGTGAGTG GTGGGTTCTC 
AACTTGACTG GGAACAGAAT CTACAACCAG GAAGCGCAGG CCCTGGAGCT GGTGGTCTTC 
AACGACAAGG TCAGCCCCCC CAGCCTGGGG TTCCTGGCCG GCTACGGTAT CATGGGCCTC 
TACGCTTCCG TGGTGCTGGT GATTGGGAAG TTTGTCCGGG AATTCTTCAG CGGGATTTCT 
CACTCCATCA TGTTTGAAGA ACTTCCCAAT GTGGATCGGA TTCTGAAATT GTGCACAGAC 
ATTTTTTTAG TCCGGGAGAC CGGAGAGCTG GAGCTCGAGG AGGATCTCTA TGCCAAATTA 
ATATTCCTGT ACCGGTCGCC AGAAACCATG ATCAAATGGA CCAGGGAGAA AACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_027960843.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027816644.1 Ovis aries piezo type mechanosensitive ion channel component 2 (PIEZO2), mRNA.

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CloneID	OSm73969
Clone ID Related Accession (Same CDS sequence)	XM_027960843.1
Accession Version	XM_027960843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	piezo-type mechanosensitive ion channel component 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040274.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCCAGACT TCCTGATGGA GATGCAGAAG CAGGACGAGC TCACAGGCTC CAACCTCACC 
TGTGCCTTCC GGTACAACGG GCTCTCCTTC GTCTACCTCA TCTACCTCCT GCTCATCCCC 
CTGTTCTCGG AACCAACTAA AGCCACCATG CAAGGACACA CGGGACGGTT GTTAAAGTCT 
CTGTGCTTCA CCAGTCTCTC CTTCCTGTTG CTGCACATCA TTTTCCACAT CACGTTGGCG 
AGTCTGGAGG CACAGCACCG CATCGCACCT GGTTACAACT GCTCAACCTG GGAGAAGACT 
TTCCGACAAA TCGGCTTCGA AAGCTTAAAG GGAGCCGACG CTGGCAATGG GATCCGAGTG 
TTTGTGCCTG ACGTGGGCAT GTTCATCGCC AGCCTGACCA TCTGGCTGGT CTGTAGGAAC 
ATTGTTCAGA AGCCAGTGGC CGAAGAGGCC GCGCAGTGTA ACCCGGAGTT TGAGAACGAA 
GAGTCGGCTG GAGGAGAAAA AATAGACTCC GAAGAGGCCC TGATCTATGA AGAGGACTTA 
GATGAAGGAG ACGGTGCTGA AGGCGATTTG GAGGAAAGCA CGAAATTAAA ACTCTTCCGC 
AGGCTGGCCT CCGTGGCCTC CAAGCTGAAG GAGTTTATTG GCAACATGAT AACTACAGCG 
GGGAAGGTTG TTGTGACGAT TTTACTGGGT TCATCAGGGA TGATGCTGCC CTCTCTGACC 
TCATCCGTGT ACTTCTTCGT GTTCCTGGGC CTGTGTACCT GGTGGTCCTG GTGCCGGACT 
TTCGACCCCT TGCTGTTCAG CTGTCTCTGC GTCCTGCTGG CCATCTTCAC TGCGGGACAC 
CTGATCGGAC TCTATCTGTA CCAGTTCCAG TTCTTCCAGG AAGCCGTTCC CCCCAACGAC 
TACTATGCCA GGTTGTTTGG TATCAAATCA GTCATTCAGA CGGACTGTTC CAGCACATGG 
AAGATCATTG CCAACCCCGA CCTGTCCTGG TACCACCACG CCAACCCCAT CGTCCTGCTG 
GTGATGTACT ACACGCTGGC CACTCTGATC CGAATCTGGC TGCAGGAGCC CGTTGTGCAG 
GAAGAGAAGA CCAAGGAGGA GGACAGAGCC CTGGCCTGCA GCCCCGCGCC CATCACGGCC 
GAGAGGAGGC GGAGCCTGTG GTACGCGACC CACTACCCCA CAGACGAGAG AAAACTTTTA 
TCCATGACTC AGGATGACTA CAAGCCGTCC GACGGCCTGC TGGTGACCGT GAATGGCAAC 
CCCGTGGACT ACCACACCAT CCACCCCAGC CTCCCACTGG AGAACGGCCC CGCCAAGGCT 
GACCTGTACT CCACACCACA GTACCGCTGG GAGCCTGCCA CTGCCTCCCC TGAAAAGAAG 
GAGGAAGATA ATGATGAGAA AGAAGAGTTT GAGGAGGAGC GGAGCCGGGA GGAGAAGAGG 
AGTGTCAAAG TGCACGCCAT GGTGTCTGTG TTCCAGTTTA TCATGAAACA GAGCTACATC 
TGTGCCCTCA TCGCCATGAT GGCCTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG 
CTGCTCATCT GGTCCTGCAC CCTGTGGATG ATCCGCAACC GGAGGAAGTA TGCCATGATC 
AGCTCCCCCT TCATGGTCGT TTACGGAAAC CTGCTGCTCA TACTGCAGTA CATATGGAGC 
TTCGAGCTCC CTGAGATTAA AAAGGTCCCG GGTTTCTTAG AAAAGAAGGA GCCGGGGGAG 
CTCGCCTCAA AGATTCTGTT CACCATCACA TTCTGGCTGC TGCTGAGACA GCACCTCACA 
GAGCAGAAAG CTCTCCAAGA AAAGGAAGCT CTTCTGTCTG AGGTCAAAAT CGGAAGTCAG 
GAAAATGAAG AAAAAGATGA TGAACAGGAT GTACAGGTAG ACAGCGAGGC TCAGGAGAAG 
GAGGAAGGAG AGGAGCCGAA GGAGGAGCAG CAGGAGCGGA GAGAGGAAGA GGAGGAGGAA 
GAGGCAGACG AGCAGGACGT GATGAAGGTG CTGGGGAACC TGGTGGTGGC CCTGTTCATC 
AAGTACTGGA TCTACGTCTG CGGGGGGATG TTCTTCTTCG TCAGCTTCGA GGGTAAAATC 
GTGATGTACA AGATCATCTA CATGGTACTC TTCCTCTTCT GTGTGGCCCT GTATCAGGTG 
CACTATGAAT GGTGGAGGAG GATTCTGAAG TACTTCTGGA TGTCGGTGGT TATCTACACA 
ATGCTGGTGC TTATCTTTAT ATACACATAT CAGTTTGAAA ACTTCCCAGG CTTGTGGCAA 
AACATGACTG GACTGAAAAA AGAAAAGCTG GAGGACCTTG GCCTGAAGCA GTTCACGGTG 
GCCGAGCTGT TCACGCGCAT CTTCATCCCC ACCTCCTTCC TCCTGGTCTG CATTCTGCAC 
CTGCACTACT TCCACGACCG ATTCCTGGAG CTCACCGACC TGAAGTCCAT CCCCAGCAAG 
GAGGACAGCG CTGTCTATAG GCTGGCCCAC CCCGAGGGAA GCCTTCCAGA CCTTGCCATG 
ATGAAGCTGA CTGCCAGCCT GGAGGTGGCA GAGGTCAAGC AGCTGGCTGG GCCTGGGGAG 
GAGATGCCAG GGGGATGCCC TGCAAAAGCC AAGAAGGGCG AGCCTGGCAG GGACAGCGAG 
GACGAGGATG AGGATGAGGA CGAGGAGGAT GAAGAGGAGG AGGAGACGTC AGATCTGAGG 
AACAAGTGGC ACCTGGTGAT CGACCGCCTC ACAGTGCTGC TGCTGCGATT CCTGGAGGGC 
TTCCACCAGC TGCAGACATT CCTGTGGTGG ATCCTGGAGC TGCACATCGT CAAGATTGTG 
TCCTCCTACA TCATCTGGGT GTCCGTGAAG GAGGTGTCTC TGTTCAACTA CGTGTTTCTG 
ATTTCCTGGG CTTTTGCTCT GCCGTATGCT AAGCTCCGCC GTCTGGCCTC CAGTGTCTGC 
ACCGTCTGGA CATGTGTGAT CATTGTCTGT AAGATGCTGT ACCAGCTGCA GACCATCAAG 
CCTGAGAACT TCTCTGTGAA CTGTTCGTTG CCAAATGAAA ACCAGACGAA CATCCCCCTG 
CACCAGCTGA ACAAGTCTCT GCTCTACAGC GCACCCATTG ACCCCACAGA ATGGGTGGGC 
CTCAGGAAGT CGTCTCCTCT ACTTGTCTAC CTAAGGAACA ACCTGCTGAT GCTGGCCATC 
CTGGCCTTCG AGGTCACCAT CTACCGCCAT CAGGAGTACT ACCGTGGTCG GAACAACCTC 
TCAACCCCCG TGTCTAAAAC CATCTTCCAC AACATCACAA GACTCCAGCT GGATGATGGA 
CTTGTTAACT GTGCCAAGTA TTTCATCAAT TACTTCTTCT ACAAGTTTGG TCTGGAGACC 
TGTTTCCTCA TGTCAGTTAA TGTGATTGGC CAGCGCATGG ACTTCTACGC CATGATTCAC 
GCCTGCTGGC TGATTGCCGT CCTGTACCGG CGCAGGAGGA AGGCCATCGC CGAGATCTGG 
CCCAAGTACT GCTGCTTCCT GGCTTGCATC ATCACCTTCC AGTATTTCAT CTGCATCGGC 
ATCCCCCCTG CCCCCTGTCG AGACTACCCT TGGAGGTTCA AGGGCGCCAG CTTCAATGAC 
AACATCGTCA AGTGGCTGTA CTTCCCTGAC TTCATCGTTC GACCCAACCC TGTGTTCCTC 
GTGTATGATT TCATGCTACT CCTGTGTGCC TCGCTGCAAC GGCAGATCTT TGAGGATGAG 
AATAAGGCTG CTGTACGGAT CGTGGCGGGC GACAACGTGG AAATCTGCAT GAACCTGGAT 
GCGGCCTCAT TTAGCCAGCA CAATCCCGTG CCCGACTTCA TCCACTGCAG GTCGTACCTG 
GACATGTCCA AGGTGGTCAT CTTCAGCTAC CTCTTCTGGT TCGTGCTCAC TATCATCTTC 
ATCACCGGGA CGACTCGCAT CAGCATCTTT TGCATGGGCT ACCTGGTGGC CTGCTTCTAC 
TTCCTGCTCT TCGGGGGCGA CTTGCTGCTG AAACCCATCA AGAGCATCCT GCGCTACTGG 
GACTGGCTGA TCGCTTACAA TGTCTTCGTG ATCACGATGA AAAACATACT GTCGATAGGA 
GCGTGTGGCT ATATTGAAAA ATTGGTTCAA AATAGTTGCT GGTTGATCCA AGCATTCAGC 
CTGGCCTGTA CAGTCAAGGG CTACAAAATG CCTGATGATG ACTCCTCCTG TAAACTGCCC 
AGTGGGGAGG CAGGAATCAT TTGGGACAGC ATATGCTTTG CCTTCCTGCT GCTGCAGAGA 
AGAGTGTTCA TGAGTTACTA TTTCCTGCAT GTGGTGGCAG ATATAAAAGC CTCACAGATC 
CTGGCATCCA GAGGGGCCGA GCTTTTCCAG GCCACCATTG TGAAGGCTGT GAAGGCCAGA 
ATCGAGGAGG AGAAAAAGTC CATGGACCAG CTGAAGAGGC AGATGGACCG CATCAAGGCC 
AGGCAACAGA AATACAAAAA GGGTAAGGAG AGGATGCTGA GCTTGACCCA GGAGTCGGGG 
GAAGGCCAAG ATCTCCAAAA ACTTTCTGAA GAGGATGACG AGAGAGAAGC AGACAAACAG 
AAAACCAAGG GCAAAAAAAA GCAGTGGTGG CGGCCTTGGG TTGATCATGC TTCCATGGTC 
AGGAGTGGAG ATTACTATTT GTTTGAAACG GATAGTGAAG AGGAGGAAGA GGAAGAATTA 
AAAAAGGAAG ACGAAGAGCC CCCCCGGAGG TCAGCCTTCC AGTTTGTTTA TCAAGCCTGG 
ATTACTGACC CTAAAACAGC ACTCCGACAG AGGCGCAAAG AGAAAAAAAG ATCTGCAAGA 
GAAGAACAGA AAAGAAGGAG AAAAGTATCT GGGGAGGGTC CTGTGGAGTG GGAAGACCGG 
GAAGATGAGC CAGTCAAAAA GAAGTCTGAT GGACCAGACA ACATCATCAA GAGGATCTTT 
AATATTTTGA AGTTCACCTG GGTCCTGTTT CTGGCAACCG TGGACAGTTT CACAACGTGG 
CTCAACTCCA TCTCCAGAGA GCACATCGAC ATCTCCACTG TCCTGAGGAT CGAGCGCTGC 
ATGCTGACCA GAGAGATCAA GAAGGGCAAC GTCCCAACCC GGGAGAGCAT CCACATGTAC 
TACCAGAACC ATATCATGAA CCTGTCCAGG GAGTCGGGGC TGGACACCAT CGACGACCAT 
GCTGGCGCTG CTGCGGGCGC ACAGACCGCC CACAGGATGG ACAGCTTGGA TTCCCACGAC 
AGCATCTCCA GCGAGCCCAC GCAGTGCACG ATGCTGTACT CGCGCCAGGG GACCACGGAG 
ACCATCGAGG AAGTGGAGGC TGAGCAGGAC GAGGAGACAT CCGAGGGGGC CCGGCAGGCC 
GAGTCCCCAC AGGACCCCAC GGAGGATGGT GACGTGGAGG CCCCGCCGTC CTACAGCAAG 
GCTGTCAGCT TTGAACGCCT GTCCTTCGAC TCCCGGGATG ACTCAGCTGG CCAGACCCAC 
ATGGAAGTCA GCCCGGATGA CAGCCGCTCA GATCGGCTGG AGGCCAGCAT CCTGCCACCC 
CTGACCCACG AGCTGACAGC CAGCGAGCTG CTGCTGAACA AGATGTTCCA TGACGACGAG 
CTGGAGGAGT CAGAGAAGTT CTACGTGGGG CAGCCTCGCT TCCTGCTGCT CTTCTACGCG 
CTGTACAACA CGCTGGTGGC CCGCTCCGAG ATGGTGTGCT ACTTTGTGAT CATCCTCAAC 
CACATGGTCT CCGCCTCCAT GATCACCCTC GTGCTGCCCA TCCTCATCTT CCTCTGGGCC 
ATGCTGTCCG TCCCCCGGCC CAGCAGGCGC TTCTGGATGA TGGCCATCGT CTACACGGAG 
GTGGCCATTG TGGTCAAGTA CTTCTTCCAA TTTGGGTTCT TCCCCTGGAA CAAGAATGTG 
GAACTGAACA AGGACAAGCC TTACCACCCA CCCAACATCA TCGGGGTGGA GAAGAAGGAC 
GCCTACGTGC TCTACGACCT CATCCAGCTG CTCGCACTCT TCTTCCACCG CTCCATCCTA 
AAGTGTCACG GCCTCTGGGA TGAAGACGAC ATCGTGGACA GCGGCTCGGA CAAGGTGGAC 
TCGGACGACG AGCTGTCCCT CGGCCATGGT AGGAGGGCCT CCTCCGACTC GCTCAAGTCC 
ATCAACCTGG CTGCATCCGT GGAGTCGGTA CACGTGACCT TCCCAGAGCA GCCGGCTGCC 
GTCCGGAGGA AGCGCTCCAG CAGCGGCTCC CAGACATCAC AGGAGTCCAG CTTTTCCTCG 
AATAGGTCCA AGAGAGGCAG CACGAGCACC CGGAACAGCA GCCAGAAAGG AAGCAGTGTC 
GTGAGCGTCA AGCAGAAGAG CAGACGGGAA CTTTACATGG AGAAGCTGCA GGAACACTTA 
ATCAAAGCAA AGGCCTTTAC CATAAAGAAG ACCCTGCAGC TCTACGTGCC CATCAGGCAG 
TTCTTCTACG ACCTCATCCA CCCCGACTAC AGCGCCGTCA CCGACGTGTA CGTGCTTATG 
TTCCTGGCGG ACACCGTGGA CTTCATCATC ATTGTCTTCG GCTTCTGGGC CTTCGGGAAA 
CACTCGGCTG CAGCCGACAT CACGTCCTCA CTGTCCGAGG ACCAGGTGCC AGGGCCCTTC 
CTGGTGATGG TGCTCATTCA GTTCGGGACT ATGGTGGTGG ACCGGGCGCT CTACCTCAGG 
AAGACTGTCC TGGGAAAGGT CATCTTCCAG GTCATCCTGG TGTTTGGGAT TCACTTCTGG 
ATGTTCTTCA TCCTGCCTGG CGTGACCGAG AGGAAGTTCA GCCAGAACCT GGTCGCGCAG 
CTGTGGTACT TCGTCAAGTG CGTGTACTTC GGGCTGTCCG CCTACCAGAT CCGCTGCGGC 
TACCCAACTC GGGTCCTCGG CAACTTCCTC ACCAAGAGTT ACAACTACGT CAACCTCTTC 
CTGTTCCAGG GGTTCCGCCT TGTCCCTTTC CTGACGGAGC TGAGGGCTGT GATGGACTGG 
GTGTGGACAG ACACGACCCT GAGCCTGTCC AGCTGGATCT GCGTGGAGGA CATCTATGCT 
CACATCTTCA TCCTCAAGTG TTGGCGGGAG TCGGAAAAAA GGTACCCGCA GCCCCGGGGG 
CAGAAGAAGA AGAAGGTGGT CAAGTACGGG ATGGGGGGCA TGATCATCGT GCTGCTCATC 
TGCATCGTCT GGTTCCCGCT ACTCTTCATG TCTCTGGTCA AATCCGTCGC TGGGGTTATC 
AACCAACCCC TGGACGTCTC AGTCACAATC ACCCTGGGCG GGTACCAGCC TATTTTCACA 
ATGAGTGCCC AGCAAAGCCA GCTGAAAGTT ATGGACCAGC CAATGTTTAA TAAATTCATT 
AAAGCTTTTT CTAAGGACAC TGGTGCTATG CAATTTCTGG AAAATTATGA AAAAGAAGAC 
ATAACAGTCG CAGAACTGGA AGGAAACTCA AATTCTTTAT GGACCATCAG CCCTCCCAGT 
AAGCAGAAAA TGATACACGA ACTCAAGGAC CCCAATAGTA GCTTCTCTGT TGTTTTTTCA 
TGGAGTATTC AGAGAAACAT GAGTCTGGGT GCAAAAGCAG AAATAGCAAC AGATAAGCTT 
TCTTTCCCTC TTAAAAACAA GACACGAGAG AATATTGCTA AAATGATAGC TGGCGGCGAC 
ACGGAAAGTT CCAGGACGCC CGTGACCATA GAAAGGATTT ACCCGTACTA TGTGAAAGCA 
CCCAGTGACT CTAACTCCAA ACCCATAAAG CAGCTTTTAT CTGAAAATAA TTTCATGAAC 
ATCACCATCA TTTTGTCCAG AGACAATGCG ACTAACTCGA ACAGTGAGTG GTGGGTTCTC 
AACTTGACTG GGAACAGAAT CTACAACCAG GAAGCGCAGG CCCTGGAGCT GGTGGTCTTC 
AACGACAAGG TCAGCCCCCC CAGCCTGGGG TTCCTGGCCG GCTACGGTAT CATGGGCCTC 
TACGCTTCCG TGGTGCTGGT GATTGGGAAG TTTGTCCGGG AATTCTTCAG CGGGATTTCT 
CACTCCATCA TGTTTGAAGA ACTTCCCAAT GTGGATCGGA TTCTGAAATT GTGCACAGAC 
ATTTTTTTAG TCCGGGAGAC CGGAGAGCTG GAGCTCGAGG AGGATCTCTA TGCCAAATTA 
ATATTCCTAT ACCGGTCGCC AGAAACCATG ATCAAATGGA CCAGGGAGAA AACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027816644.1
CDS	1..8160
Translation	MPDFLMEMQKQDELTGSNLTCAFRYNGLSFVYLIYLLLIPLFSEPTKATMQGHTGRLLKSLCFTSLSFLLLHIIFHITLASLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDVGMFIASLTIWLVCRNIVQKPVAEEAAQCNPEFENEESAGGEKIDSEEALIYEEDLDEGDGAEGDLEESTKLKLFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIANPDLSWYHHANPIVLLVMYYTLATLIRIWLQEPVVQEEKTKEEDRALACSPAPITAERRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPLENGPAKADLYSTPQYRWEPATASPEKKEEDNDEKEEFEEERSREEKRSVKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDDEQDVQVDSEAQEKEEGEEPKEEQQERREEEEEEEADEQDVMKVLGNLVVALFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRRILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDSAVYRLAHPEGSLPDLAMMKLTASLEVAEVKQLAGPGEEMPGGCPAKAKKGEPGRDSEDEDEDEDEEDEEEEETSDLRNKWHLVIDRLTVLLLRFLEGFHQLQTFLWWILELHIVKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPLHQLNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLSTPVSKTIFHNITRLQLDDGLVNCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIVKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIVAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVVIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIEKLVQNSCWLIQAFSLACTVKGYKMPDDDSSCKLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQESGEGQDLQKLSEEDDEREADKQKTKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRRKEKKRSAREEQKRRRKVSGEGPVEWEDREDEPVKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDDHAGAAAGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQDEETSEGARQAESPQDPTEDGDVEAPPSYSKAVSFERLSFDSRDDSAGQTHMEVSPDDSRSDRLEASILPPLTHELTASELLLNKMFHDDELEESEKFYVGQPRFLLLFYALYNTLVARSEMVCYFVIILNHMVSASMITLVLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVELNKDKPYHPPNIIGVEKKDAYVLYDLIQLLALFFHRSILKCHGLWDEDDIVDSGSDKVDSDDELSLGHGRRASSDSLKSINLAASVESVHVTFPEQPAAVRRKRSSSGSQTSQESSFSSNRSKRGSTSTRNSSQKGSSVVSVKQKSRRELYMEKLQEHLIKAKAFTIKKTLQLYVPIRQFFYDLIHPDYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLVKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQPMFNKFIKAFSKDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELKDPNSSFSVVFSWSIQRNMSLGAKAEIATDKLSFPLKNKTRENIAKMIAGGDTESSRTPVTIERIYPYYVKAPSDSNSKPIKQLLSENNFMNITIILSRDNATNSNSEWWVLNLTGNRIYNQEAQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_027960843.1
Organism	Ovis aries(sheep)
Definition	Ovis aries piezo type mechanosensitive ion channel component 2 (PIEZO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027960843.1

ORF Insert Sequence:

ATGCCAGACT TCCTGATGGA GATGCAGAAG CAGGACGAGC TCACAGGCTC CAACCTCACC 
TGTGCCTTCC GGTACAACGG GCTCTCCTTC GTCTACCTCA TCTACCTCCT GCTCATCCCC 
CTGTTCTCGG AACCAACTAA AGCCACCATG CAAGGACACA CGGGACGGTT GTTAAAGTCT 
CTGTGCTTCA CCAGTCTCTC CTTCCTGTTG CTGCACATCA TTTTCCACAT CACGTTGGCG 
AGTCTGGAGG CACAGCACCG CATCGCACCT GGTTACAACT GCTCAACCTG GGAGAAGACT 
TTCCGACAAA TCGGCTTCGA AAGCTTAAAG GGAGCCGACG CTGGCAATGG GATCCGAGTG 
TTTGTGCCTG ACGTGGGCAT GTTCATCGCC AGCCTGACCA TCTGGCTGGT CTGTAGGAAC 
ATTGTTCAGA AGCCAGTGGC CGAAGAGGCC GCGCAGTGTA ACCCGGAGTT TGAGAACGAA 
GAGTCGGCTG GAGGAGAAAA AATAGACTCC GAAGAGGCCC TGATCTATGA AGAGGACTTA 
GATGAAGGAG ACGGTGCTGA AGGCGATTTG GAGGAAAGCA CGAAATTAAA ACTCTTCCGC 
AGGCTGGCCT CCGTGGCCTC CAAGCTGAAG GAGTTTATTG GCAACATGAT AACTACAGCG 
GGGAAGGTTG TTGTGACGAT TTTACTGGGT TCATCAGGGA TGATGCTGCC CTCTCTGACC 
TCATCCGTGT ACTTCTTCGT GTTCCTGGGC CTGTGTACCT GGTGGTCCTG GTGCCGGACT 
TTCGACCCCT TGCTGTTCAG CTGTCTCTGC GTCCTGCTGG CCATCTTCAC TGCGGGACAC 
CTGATCGGAC TCTATCTGTA CCAGTTCCAG TTCTTCCAGG AAGCCGTTCC CCCCAACGAC 
TACTATGCCA GGTTGTTTGG TATCAAATCA GTCATTCAGA CGGACTGTTC CAGCACATGG 
AAGATCATTG CCAACCCCGA CCTGTCCTGG TACCACCACG CCAACCCCAT CGTCCTGCTG 
GTGATGTACT ACACGCTGGC CACTCTGATC CGAATCTGGC TGCAGGAGCC CGTTGTGCAG 
GAAGAGAAGA CCAAGGAGGA GGACAGAGCC CTGGCCTGCA GCCCCGCGCC CATCACGGCC 
GAGAGGAGGC GGAGCCTGTG GTACGCGACC CACTACCCCA CAGACGAGAG AAAACTTTTA 
TCCATGACTC AGGATGACTA CAAGCCGTCC GACGGCCTGC TGGTGACCGT GAATGGCAAC 
CCCGTGGACT ACCACACCAT CCACCCCAGC CTCCCACTGG AGAACGGCCC CGCCAAGGCT 
GACCTGTACT CCACACCACA GTACCGCTGG GAGCCTGCCA CTGCCTCCCC TGAAAAGAAG 
GAGGAAGATA ATGATGAGAA AGAAGAGTTT GAGGAGGAGC GGAGCCGGGA GGAGAAGAGG 
AGTGTCAAAG TGCACGCCAT GGTGTCTGTG TTCCAGTTTA TCATGAAACA GAGCTACATC 
TGTGCCCTCA TCGCCATGAT GGCCTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG 
CTGCTCATCT GGTCCTGCAC CCTGTGGATG ATCCGCAACC GGAGGAAGTA TGCCATGATC 
AGCTCCCCCT TCATGGTCGT TTACGGAAAC CTGCTGCTCA TACTGCAGTA CATATGGAGC 
TTCGAGCTCC CTGAGATTAA AAAGGTCCCG GGTTTCTTAG AAAAGAAGGA GCCGGGGGAG 
CTCGCCTCAA AGATTCTGTT CACCATCACA TTCTGGCTGC TGCTGAGACA GCACCTCACA 
GAGCAGAAAG CTCTCCAAGA AAAGGAAGCT CTTCTGTCTG AGGTCAAAAT CGGAAGTCAG 
GAAAATGAAG AAAAAGATGA TGAACAGGAT GTACAGGTAG ACAGCGAGGC TCAGGAGAAG 
GAGGAAGGAG AGGAGCCGAA GGAGGAGCAG CAGGAGCGGA GAGAGGAAGA GGAGGAGGAA 
GAGGCAGACG AGCAGGACGT GATGAAGGTG CTGGGGAACC TGGTGGTGGC CCTGTTCATC 
AAGTACTGGA TCTACGTCTG CGGGGGGATG TTCTTCTTCG TCAGCTTCGA GGGTAAAATC 
GTGATGTACA AGATCATCTA CATGGTACTC TTCCTCTTCT GTGTGGCCCT GTATCAGGTG 
CACTATGAAT GGTGGAGGAG GATTCTGAAG TACTTCTGGA TGTCGGTGGT TATCTACACA 
ATGCTGGTGC TTATCTTTAT ATACACATAT CAGTTTGAAA ACTTCCCAGG CTTGTGGCAA 
AACATGACTG GACTGAAAAA AGAAAAGCTG GAGGACCTTG GCCTGAAGCA GTTCACGGTG 
GCCGAGCTGT TCACGCGCAT CTTCATCCCC ACCTCCTTCC TCCTGGTCTG CATTCTGCAC 
CTGCACTACT TCCACGACCG ATTCCTGGAG CTCACCGACC TGAAGTCCAT CCCCAGCAAG 
GAGGACAGCG CTGTCTATAG GCTGGCCCAC CCCGAGGGAA GCCTTCCAGA CCTTGCCATG 
ATGAAGCTGA CTGCCAGCCT GGAGGTGGCA GAGGTCAAGC AGCTGGCTGG GCCTGGGGAG 
GAGATGCCAG GGGGATGCCC TGCAAAAGCC AAGAAGGGCG AGCCTGGCAG GGACAGCGAG 
GACGAGGATG AGGATGAGGA CGAGGAGGAT GAAGAGGAGG AGGAGACGTC AGATCTGAGG 
AACAAGTGGC ACCTGGTGAT CGACCGCCTC ACAGTGCTGC TGCTGCGATT CCTGGAGGGC 
TTCCACCAGC TGCAGACATT CCTGTGGTGG ATCCTGGAGC TGCACATCGT CAAGATTGTG 
TCCTCCTACA TCATCTGGGT GTCCGTGAAG GAGGTGTCTC TGTTCAACTA CGTGTTTCTG 
ATTTCCTGGG CTTTTGCTCT GCCGTATGCT AAGCTCCGCC GTCTGGCCTC CAGTGTCTGC 
ACCGTCTGGA CATGTGTGAT CATTGTCTGT AAGATGCTGT ACCAGCTGCA GACCATCAAG 
CCTGAGAACT TCTCTGTGAA CTGTTCGTTG CCAAATGAAA ACCAGACGAA CATCCCCCTG 
CACCAGCTGA ACAAGTCTCT GCTCTACAGC GCACCCATTG ACCCCACAGA ATGGGTGGGC 
CTCAGGAAGT CGTCTCCTCT ACTTGTCTAC CTAAGGAACA ACCTGCTGAT GCTGGCCATC 
CTGGCCTTCG AGGTCACCAT CTACCGCCAT CAGGAGTACT ACCGTGGTCG GAACAACCTC 
TCAACCCCCG TGTCTAAAAC CATCTTCCAC AACATCACAA GACTCCAGCT GGATGATGGA 
CTTGTTAACT GTGCCAAGTA TTTCATCAAT TACTTCTTCT ACAAGTTTGG TCTGGAGACC 
TGTTTCCTCA TGTCAGTTAA TGTGATTGGC CAGCGCATGG ACTTCTACGC CATGATTCAC 
GCCTGCTGGC TGATTGCCGT CCTGTACCGG CGCAGGAGGA AGGCCATCGC CGAGATCTGG 
CCCAAGTACT GCTGCTTCCT GGCTTGCATC ATCACCTTCC AGTATTTCAT CTGCATCGGC 
ATCCCCCCTG CCCCCTGTCG AGACTACCCT TGGAGGTTCA AGGGCGCCAG CTTCAATGAC 
AACATCGTCA AGTGGCTGTA CTTCCCTGAC TTCATCGTTC GACCCAACCC TGTGTTCCTC 
GTGTATGATT TCATGCTACT CCTGTGTGCC TCGCTGCAAC GGCAGATCTT TGAGGATGAG 
AATAAGGCTG CTGTACGGAT CGTGGCGGGC GACAACGTGG AAATCTGCAT GAACCTGGAT 
GCGGCCTCAT TTAGCCAGCA CAATCCCGTG CCCGACTTCA TCCACTGCAG GTCGTACCTG 
GACATGTCCA AGGTGGTCAT CTTCAGCTAC CTCTTCTGGT TCGTGCTCAC TATCATCTTC 
ATCACCGGGA CGACTCGCAT CAGCATCTTT TGCATGGGCT ACCTGGTGGC CTGCTTCTAC 
TTCCTGCTCT TCGGGGGCGA CTTGCTGCTG AAACCCATCA AGAGCATCCT GCGCTACTGG 
GACTGGCTGA TCGCTTACAA TGTCTTCGTG ATCACGATGA AAAACATACT GTCGATAGGA 
GCGTGTGGCT ATATTGAAAA ATTGGTTCAA AATAGTTGCT GGTTGATCCA AGCATTCAGC 
CTGGCCTGTA CAGTCAAGGG CTACAAAATG CCTGATGATG ACTCCTCCTG TAAACTGCCC 
AGTGGGGAGG CAGGAATCAT TTGGGACAGC ATATGCTTTG CCTTCCTGCT GCTGCAGAGA 
AGAGTGTTCA TGAGTTACTA TTTCCTGCAT GTGGTGGCAG ATATAAAAGC CTCACAGATC 
CTGGCATCCA GAGGGGCCGA GCTTTTCCAG GCCACCATTG TGAAGGCTGT GAAGGCCAGA 
ATCGAGGAGG AGAAAAAGTC CATGGACCAG CTGAAGAGGC AGATGGACCG CATCAAGGCC 
AGGCAACAGA AATACAAAAA GGGTAAGGAG AGGATGCTGA GCTTGACCCA GGAGTCGGGG 
GAAGGCCAAG ATCTCCAAAA ACTTTCTGAA GAGGATGACG AGAGAGAAGC AGACAAACAG 
AAAACCAAGG GCAAAAAAAA GCAGTGGTGG CGGCCTTGGG TTGATCATGC TTCCATGGTC 
AGGAGTGGAG ATTACTATTT GTTTGAAACG GATAGTGAAG AGGAGGAAGA GGAAGAATTA 
AAAAAGGAAG ACGAAGAGCC CCCCCGGAGG TCAGCCTTCC AGTTTGTTTA TCAAGCCTGG 
ATTACTGACC CTAAAACAGC ACTCCGACAG AGGCGCAAAG AGAAAAAAAG ATCTGCAAGA 
GAAGAACAGA AAAGAAGGAG AAAAGTATCT GGGGAGGGTC CTGTGGAGTG GGAAGACCGG 
GAAGATGAGC CAGTCAAAAA GAAGTCTGAT GGACCAGACA ACATCATCAA GAGGATCTTT 
AATATTTTGA AGTTCACCTG GGTCCTGTTT CTGGCAACCG TGGACAGTTT CACAACGTGG 
CTCAACTCCA TCTCCAGAGA GCACATCGAC ATCTCCACTG TCCTGAGGAT CGAGCGCTGC 
ATGCTGACCA GAGAGATCAA GAAGGGCAAC GTCCCAACCC GGGAGAGCAT CCACATGTAC 
TACCAGAACC ATATCATGAA CCTGTCCAGG GAGTCGGGGC TGGACACCAT CGACGACCAT 
GCTGGCGCTG CTGCGGGCGC ACAGACCGCC CACAGGATGG ACAGCTTGGA TTCCCACGAC 
AGCATCTCCA GCGAGCCCAC GCAGTGCACG ATGCTGTACT CGCGCCAGGG GACCACGGAG 
ACCATCGAGG AAGTGGAGGC TGAGCAGGAC GAGGAGACAT CCGAGGGGGC CCGGCAGGCC 
GAGTCCCCAC AGGACCCCAC GGAGGATGGT GACGTGGAGG CCCCGCCGTC CTACAGCAAG 
GCTGTCAGCT TTGAACGCCT GTCCTTCGAC TCCCGGGATG ACTCAGCTGG CCAGACCCAC 
ATGGAAGTCA GCCCGGATGA CAGCCGCTCA GATCGGCTGG AGGCCAGCAT CCTGCCACCC 
CTGACCCACG AGCTGACAGC CAGCGAGCTG CTGCTGAACA AGATGTTCCA TGACGACGAG 
CTGGAGGAGT CAGAGAAGTT CTACGTGGGG CAGCCTCGCT TCCTGCTGCT CTTCTACGCG 
CTGTACAACA CGCTGGTGGC CCGCTCCGAG ATGGTGTGCT ACTTTGTGAT CATCCTCAAC 
CACATGGTCT CCGCCTCCAT GATCACCCTC GTGCTGCCCA TCCTCATCTT CCTCTGGGCC 
ATGCTGTCCG TCCCCCGGCC CAGCAGGCGC TTCTGGATGA TGGCCATCGT CTACACGGAG 
GTGGCCATTG TGGTCAAGTA CTTCTTCCAA TTTGGGTTCT TCCCCTGGAA CAAGAATGTG 
GAACTGAACA AGGACAAGCC TTACCACCCA CCCAACATCA TCGGGGTGGA GAAGAAGGAC 
GCCTACGTGC TCTACGACCT CATCCAGCTG CTCGCACTCT TCTTCCACCG CTCCATCCTA 
AAGTGTCACG GCCTCTGGGA TGAAGACGAC ATCGTGGACA GCGGCTCGGA CAAGGTGGAC 
TCGGACGACG AGCTGTCCCT CGGCCATGGT AGGAGGGCCT CCTCCGACTC GCTCAAGTCC 
ATCAACCTGG CTGCATCCGT GGAGTCGGTA CACGTGACCT TCCCAGAGCA GCCGGCTGCC 
GTCCGGAGGA AGCGCTCCAG CAGCGGCTCC CAGACATCAC AGGAGTCCAG CTTTTCCTCG 
AATAGGTCCA AGAGAGGCAG CACGAGCACC CGGAACAGCA GCCAGAAAGG AAGCAGTGTC 
GTGAGCGTCA AGCAGAAGAG CAGACGGGAA CTTTACATGG AGAAGCTGCA GGAACACTTA 
ATCAAAGCAA AGGCCTTTAC CATAAAGAAG ACCCTGCAGC TCTACGTGCC CATCAGGCAG 
TTCTTCTACG ACCTCATCCA CCCCGACTAC AGCGCCGTCA CCGACGTGTA CGTGCTTATG 
TTCCTGGCGG ACACCGTGGA CTTCATCATC ATTGTCTTCG GCTTCTGGGC CTTCGGGAAA 
CACTCGGCTG CAGCCGACAT CACGTCCTCA CTGTCCGAGG ACCAGGTGCC AGGGCCCTTC 
CTGGTGATGG TGCTCATTCA GTTCGGGACT ATGGTGGTGG ACCGGGCGCT CTACCTCAGG 
AAGACTGTCC TGGGAAAGGT CATCTTCCAG GTCATCCTGG TGTTTGGGAT TCACTTCTGG 
ATGTTCTTCA TCCTGCCTGG CGTGACCGAG AGGAAGTTCA GCCAGAACCT GGTCGCGCAG 
CTGTGGTACT TCGTCAAGTG CGTGTACTTC GGGCTGTCCG CCTACCAGAT CCGCTGCGGC 
TACCCAACTC GGGTCCTCGG CAACTTCCTC ACCAAGAGTT ACAACTACGT CAACCTCTTC 
CTGTTCCAGG GGTTCCGCCT TGTCCCTTTC CTGACGGAGC TGAGGGCTGT GATGGACTGG 
GTGTGGACAG ACACGACCCT GAGCCTGTCC AGCTGGATCT GCGTGGAGGA CATCTATGCT 
CACATCTTCA TCCTCAAGTG TTGGCGGGAG TCGGAAAAAA GGTACCCGCA GCCCCGGGGG 
CAGAAGAAGA AGAAGGTGGT CAAGTACGGG ATGGGGGGCA TGATCATCGT GCTGCTCATC 
TGCATCGTCT GGTTCCCGCT ACTCTTCATG TCTCTGGTCA AATCCGTCGC TGGGGTTATC 
AACCAACCCC TGGACGTCTC AGTCACAATC ACCCTGGGCG GGTACCAGCC TATTTTCACA 
ATGAGTGCCC AGCAAAGCCA GCTGAAAGTT ATGGACCAGC CAATGTTTAA TAAATTCATT 
AAAGCTTTTT CTAAGGACAC TGGTGCTATG CAATTTCTGG AAAATTATGA AAAAGAAGAC 
ATAACAGTCG CAGAACTGGA AGGAAACTCA AATTCTTTAT GGACCATCAG CCCTCCCAGT 
AAGCAGAAAA TGATACACGA ACTCAAGGAC CCCAATAGTA GCTTCTCTGT TGTTTTTTCA 
TGGAGTATTC AGAGAAACAT GAGTCTGGGT GCAAAAGCAG AAATAGCAAC AGATAAGCTT 
TCTTTCCCTC TTAAAAACAA GACACGAGAG AATATTGCTA AAATGATAGC TGGCGGCGAC 
ACGGAAAGTT CCAGGACGCC CGTGACCATA GAAAGGATTT ACCCGTACTA TGTGAAAGCA 
CCCAGTGACT CTAACTCCAA ACCCATAAAG CAGCTTTTAT CTGAAAATAA TTTCATGAAC 
ATCACCATCA TTTTGTCCAG AGACAATGCG ACTAACTCGA ACAGTGAGTG GTGGGTTCTC 
AACTTGACTG GGAACAGAAT CTACAACCAG GAAGCGCAGG CCCTGGAGCT GGTGGTCTTC 
AACGACAAGG TCAGCCCCCC CAGCCTGGGG TTCCTGGCCG GCTACGGTAT CATGGGCCTC 
TACGCTTCCG TGGTGCTGGT GATTGGGAAG TTTGTCCGGG AATTCTTCAG CGGGATTTCT 
CACTCCATCA TGTTTGAAGA ACTTCCCAAT GTGGATCGGA TTCTGAAATT GTGCACAGAC 
ATTTTTTTAG TCCGGGAGAC CGGAGAGCTG GAGCTCGAGG AGGATCTCTA TGCCAAATTA 
ATATTCCTAT ACCGGTCGCC AGAAACCATG ATCAAATGGA CCAGGGAGAA AACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PIEZO2 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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