OTOF cDNA ORF clone, Otolemur garnettii(small-eared galago)

Gene
Clones
gRNAs

Summary

Gene Symbol	OTOF
Entrez Gene ID	100957414
Full Name	otoferlin
Gene Type	protein-coding
Organism	Otolemur garnettii(small-eared galago)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012803709.1	XP_012659163.1	otoferlin

Pathways from BioSystems
Gene Ontology

The following OTOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OTOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOt07354	XM_012803709.1 Latest version!	Otolemur garnettii otoferlin (OTOF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OTOF ( XM_012803709.1 ) cDNA ORF clone, Otolemur garnettii(small-eared galago) -> XP_012659163.1 Otolemur garnettii otoferlin (OTOF), mRNA.

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CloneID	OOt07354
Clone ID Related Accession (Same CDS sequence)	XM_012803709.1
Accession Version	XM_012803709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515600000000
Organism	Otolemur garnettii(small-eared galago)
Product	otoferlin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003852417.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Otolemur garnettii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCCCTGT TCGTCCACCT CAAGACAGTC TTGGAGCTGC GGGGCAAGGG CGACCGCATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TCAATGAGGC CCACACTGGC CTCAAGTCCT GGGCCACCAG CATGCTCACA 
GCCTCTCATC TCTGGTTGCT TCCTGAGGAG GTGGTGGTGG GAGGGCTAAT CGGGACCTTC 
CGAATGGTGC TGCAGAAGGT GGTGGAGGAG AGCCATGTGG AGGTGACTGA CACCCTGATT 
GATGACAATA ATGCTATCAT CAAGACCAGC CTGTGCGTGG AGGTCCGGTA TCGAGCCACA 
GATGGCACTG TGGGCTCCTG GGATGATGGG GACTTCCTGG GGGATGAGTC TCTTCAAGAG 
GAAGAGAAGG ACAGCCAGGA AACAGACGGA CTGCTGCCTG GTTCCCGTCC CAGTTCCCGG 
CCACCAGGAG AGAAGAGCTT CCGGAGCAAA GGCAGAGAGA AGACCAAGGG AGGCAGAGAT 
GGCGAGTACA AAGCCGGGAG GAGCGTGTTC TCCGCCATGA AGCTCGGTAA GAGCCGGACC 
CACAAGGAGG AGCCCCAAAG ACAAGACGAA CCAGCAGTGC TGGAGATGGA GGACCTGGAC 
CATCTGGCCA TTCGGCTAGG GGACAGGCTG GATCCTGACT CTGTGTCTCT GGCCTCAGTC 
ACAGCTCTCA CTACCAATGT CTCTAACAAG CGATCTAAGC CAGACATAAA GATGGAGCCA 
AGTGCTGGGC GGCCCATGGA TTACCAGGTC AGCATCACGG TGATTGAGGC TCGGCAGCTG 
GTGGGCTTGA ACATGGACCC CGTGGTGTGT GTGGAGGTGG GCGATGACAA GAAGTACACA 
TCCATGAAGG AGTCCACTAA CTGCCCCTAT TACAATGAGT ACTTCGTGTT CGATTTCCAT 
GTCTCTCCTG ATGTTATGTT CGACAAAATC ATCAAGATCT CGGTGATTCA CTCCAAGAAC 
CTGCTCCGCA GCGGCACCCT GGTGGGCTCC TTCAAAATGG ATGTGGGGAC CGTGTACTCC 
CAGCCAGAGC ACCAGTTCCA TCACAAGTGG GCCATCCTGT CAGACCCTGA TGACATCTCT 
GCGGGGCTAA AGGGCTATGT GAAGTGCGAC GTCGCCGTCG TGGGCAAGGG GGACAACATC 
AAGACGCCCC ACAAGGCCAA TGAGACAGAT GAGGATGACA TTGAAGGAAA TATTCTGGGC 
CCTTCCGGGA TCCCACCAGC AAGCAGGTGG GCCCGGTTCT ATGTGAAAAT TTACCGAGCA 
GAGGGGCTGC CTCGTATGAA CACAAGCCTC ATGGCCAACG TGAAGAAGGC TTTCATTGGT 
GAAAACAAGG ACCTTGTGGA CCCCTACGTG CAAGTCTTCT TTGCTGGCCA GAAGGGCAAG 
ACTTCAGTGC AGAAGAGCAG CTACGAGCCA CTGTGGAATG AGCAGGTCGT CTTCACAGAC 
CTCTTCCCTC CTCTCTGCAA ACGCATGAAG GTACAGATCC GAGACTCAGA CAAGGTCAAT 
GATGTGGCCA TCGGCACCCA CTTCATAGAC CTGCGCAAGA TTTCCAATGA TGGAGACAAA 
GGCTTCCTGC CCACACTGGG CCCTGCCTGG GTGAACATGT ATGGCTCCAC ACGCAACTAC 
ACGCTGCTGG ACGAGCACCA GGACTTGAAT GAGGGCCTGG GCGAGGGTGT GTCTTTCCGT 
GCCCGTCTCC TGCTAGGCCT GGCAGTGGAG ATCCTGGACA CCTCCAACCC TGAGCTCACC 
AGCTCCACAG AGGTACAATT GGAGCAGGCC ACACCCATCT CAGAGAGCTG CACTGGGAAA 
ATGGACGAGT TCTTTCTCTT TGGAGCCTTC CTGGAGGCTT CAATGATCGA CCGGAAAAAT 
GGAGACAAGC CAATCACCTT TGAGGTCACC ATAGGCAACT ATGGGAACGA AGTGGATGGA 
CTGTCACGAC CCCAATGGTC ACGGCCACGG AAGGAGCCCG GGGATGAGGA GGAAGTAGAC 
TTGATTCAGA ACTCCAGTGA TGATGAGGGT AGTGACAATG GGGACCTGGC CTCAGTCTCT 
TCCACACCAC CCATGCGGCC CCAGGTCACT GACAGGAACT ACTTCCATCT GCCCTACCTG 
GAGCGCAAGC CCTGCATCTA CATCAAGAGC TGGTGGCCAG ACCAGCGTCG TCGCCTCTAC 
AACGCCAATG TCATGGACCA CATTGCTGAC AAGCTGGAGG AAGGCCTGAA TGACGTGCAG 
GAGATGATCA AGACAGAGAA GTCCTACCCG GAGCGTCGCC TGCGGGGTGT CCTGGAGGAG 
CTCAGCTGTG GCTGCTACCG TTTCCTCTCC CTCGCGGACA AGGACCAGAG CCACTCCTCC 
CGTACCAGGC TTGACCGGGA GCGCCTCAAG TCCTGCATGA GGGAGCTGGA GAACATGGGA 
CACCAGGCAC GGACCCTGCG GGCCCAGGTG AAGCGGCACA CGGTGCGGGA CAAGCTGAGG 
CTGTGCCAGA ACTTCCTGCA GAAGCTGCGC TTCCTGGCAG ATGAGCCTCA GCATAGCATC 
CCCGACGTCT TCATCTGGAT GATGAGCAAC AACAAGCGCA TCGCCTACTC GCGGGTGCCC 
TCCAAGGACC TGCTCTTCTC CATCGTGGAG GAGGAGATGG GCAAGGACTG CGCCAAGGTC 
AAGACGCTCT TCCTGAAGCT GCCGGGAAAG CGAGGCTTTG GCTCAGCCGG CTGGACGGTG 
CAGGCTAAGC TGGAGCTATA TCTGTGGCTG GGCCTCAGCA AGCAGCGCAA GGAATTCCTG 
TGTGGCCTGC CTTGTGGCTT CGAGGAGGTC AAGGCGGCCC AGGGCCTGGG CCTGCACTCC 
TTCCCCCCAA TCAGCCTGGT CTACACCAAG AAGCAGGCCT TCCAGCTTCG AGCCCACATG 
TACCAGGCCC GCAGCCTGTT TGCCGCTGAC AGCAGTGGGC TGTCCGACCC CTTCGCCCGT 
GTCTTCTTCA TCAACCAGAG TCAGTGCACA GAGGTGCTAA ATGAAACCCT GTGTCCCACC 
TGGGACCAGA TGCTGGTGTT CGACAACCTG GAGCTGTATG GTGAAGCTCA CGAGTTGCGG 
GATGACCCCC CCATCATTGT CATAGAAATC TATGACCAGG ATACCATGGG CAAAGCCGAC 
TTCATGGGCC GCACCTTTGC CAAGCCTGTG GTGAAGATGG CAGACGAGGC GTACTGCCCT 
CCACGCTTCC CGCCCCAGCT CGAGTACTAC CAGATCTACC GCGGCAACGC CACGGCGGGA 
GACCTGCTGG CCGCCTTCGA GCTGCTACAG ATTGGGCCAG CAGGGAAGGC TGACCTACCA 
CCCATCGATG GCCCAGTGGA TGTGGACCGA GGGCCCATCA TGCCTGTGCC TGTGGGCATC 
CGGCCCGTGC TCAGCAAGTA CCGTGTGGAG GTACTATTCT GGGGTCTGCG GGATCTGAAG 
CGAGTGAACT TGGCTCAGGT GGACCGGCCA CGGGTAGACA TCGAGTGTGC AGGAAAGGGG 
GTACAGTCGT CCCTGATCCA CAATTACAAG AAAAACCCGA ACTTCAACAC CCTGGTCAAG 
TGGTTTGAAG TGGACCTCCC AGAGAATGAG CTCCTGCACC CACCCTTGAA CATCCGTGTG 
GTGGACTGCC GGGCCTTCGG CCGCTACACC CTGGTGGGCT CCCATGCCGT CAGCTCCCTG 
CGTCGTTTCA TCTATCGGCC CCCAGACCGC TCAGCCCCCA GCTGGAACAC CACAGGGGAG 
GTCGTGGTGA ACATGGAGCC CGAGGTGCCC ATCAAGAAAC TGGAGACCAT GGTGAAGCTG 
GACGCGACTT CTGATGCTGT TGTCAAGGTG GATGTGGCTG AGGAGGAGAA GGAGAAAAAG 
AAGAAGAAGA AGAAGGGCAC CACAGAAGAG CCAGAGGAGG AGGAGCCGGA TGAGGCCATG 
CTGGACTGGT GGTCCAAGTA TTTTGCCTCC ATTGACACCA TGAAGGAGCA ACTTCGACAA 
CAAGAGGCCT CAGGAATTGA CCTGGAAGAG AAGGAAGAGG TGGAAAACAG TGAAGGCCTG 
AAGGGGCCGA TGAAGGGCAA GGAGAAGACA AAGGCGGCCA AGGAAGAAAA GAAGAAGAAG 
AACCAGAGCC CTGGTCCTGG TCAGGGGTCT GAGGTCCCAG AGAAAAAGAA ACCCAAGATT 
GATGAGCTTA AGGTGTACCC CAAGGAGCTA GAGTCTGAGT TCGACAACTT TGAGGACTGG 
CTTCACACTT TCAACCTGCT GCGGGGCAAG ACTGGGGATG ATGAGGATGG CTCTACCGAG 
GAGGAGCGCA TCGTGGGCCG GTTCAAGGGA TCCCTCTGCG TGTACAAAGT ACCACTCCCA 
GAGGATGTAT CCCGGGAAGC TGGCTACGAC CCCACCTGTG GCATGTTCCA GGGCATCCCA 
AGCAATGACC CCATCAACGT GCTGGTCCGA GTCTATGTGG TCCGGGCCAC GGACCTGCAC 
CCCGCAGACA TCAACGGCAA AGCCGACCCC TACATCGCCA TCCGGCTAGG CAAGACTGAC 
ATCCGCGACA AGGAGAACTA CATCTCCAAG CAGCTCAACC CTGTGTTTGG AAAGTCATTT 
GACATCGAAG CCTCCTTCCC CATGGAATCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC 
CTGGTAGGTA CAGATGACCT CATTGGGGAA ACCAAGATTG ACCTAGAGAA CCGCTTCTAC 
AGCAAGCACC GAGCCACTTG TGGCATCACC CAGACCTACT CCATACATGG CTACAATATC 
TGGCGAGACC CCATGAAGCC CAGCCAGATC CTGACCCGCC TCTGCAAAGA CGGCAAAGTG 
GACGGCCCTC ACTTTGGGCC CCCTGGGAGA GTGAAGGTGG CCAACCGTGT CTTCACTGGG 
CCTTCTGAGA TCGAGGATGA GAATGGTCAG AGAAAGCCCA CAGACGAGCA CGTGGCATTG 
TGCGCCCTGA GGCACTGGGA GGACATCCCC CGTGTGGGCT GTCGACTGGT GCCAGAACAT 
GTGGAGACAA GGCCACTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG 
CTGTGGGTGG ACATGTTCCC CATGGACATG CCAGCCCCTG GGACACCTCT GGACATCTCT 
CCCCGGAAAC CCAAGAAGTA TGAGCTGCGG GTCATAGTGT GGAACACAGA TGAGGTGGTC 
CTGGAGGATG ATGATTTCTT CACTGGGGAG AAGTCCAGTG ACATTTTTGT GCGGGGGTGG 
CTGAAGGGCC AGCAGGAGGA CAAGCAAGAC ACAGATGTCC ACTACCATTC CCTTACTGGT 
GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTTGACT ACCTGGCAGC GGAGGAAAAG 
ATCGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGATG AGACTGAATA CAAGATCCCC 
GCGCGGCTCA CTCTGCAGAT CTGGGATGCT GACCACTTCT CTGCTGATGA CTTCCTGGGG 
GCCATCGAGC TGGACCTGAA CCGGTTCCCA CGGGGTGCAA AGACAGCCAA GCAGTGCACC 
ATGGAGATGG CCACCGGGGA GGTGGACGTG CCTCTGGTTT CCATCTTCAA GCAGAAGCGT 
GTCAAAGGCT GGTGGCCCCT CCTGGCTCGC AATGAGAATG ACGAGTTTGA GCTCACGGGC 
AAGGTGGAGG CTGAGTTGCA TTTACTGACA GCAGAGGAGG CAGAGAAGAA TCCAGTGGGC 
CTGGCCCGCA ATGAACCTGA CCCCCTGGAG AAACCCAACC GGCCCGACAC AGCCTTCGTC 
TGGTTCCTCA ACCCCCTGAA GTCCATCAAG TACCTGATCT GCACCCGGTA CAAGTGGCTC 
ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTCATGC TGGCCCTCTT CCTCTACAGC 
ATGCCCGGCT ACATGGTCAA GAAGCTCCTG GGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012659163.1
CDS	1..5979
Translation	MALFVHLKTVLELRGKGDRIAKVTFRGQSFYSRVLENCEDVADFNEAHTGLKSWATSMLTASHLWLLPEEVVVGGLIGTFRMVLQKVVEESHVEVTDTLIDDNNAIIKTSLCVEVRYRATDGTVGSWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSSRPPGEKSFRSKGREKTKGGRDGEYKAGRSVFSAMKLGKSRTHKEEPQRQDEPAVLEMEDLDHLAIRLGDRLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNILGPSGIPPASRWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLLLGLAVEILDTSNPELTSSTEVQLEQATPISESCTGKMDEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGLSRPQWSRPRKEPGDEEEVDLIQNSSDDEGSDNGDLASVSSTPPMRPQVTDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANVMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCYRFLSLADKDQSHSSRTRLDRERLKSCMRELENMGHQARTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYSRVPSKDLLFSIVEEEMGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKEFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPVVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPIDGPVDVDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTGEVVVNMEPEVPIKKLETMVKLDATSDAVVKVDVAEEEKEKKKKKKKGTTEEPEEEEPDEAMLDWWSKYFASIDTMKEQLRQQEASGIDLEEKEEVENSEGLKGPMKGKEKTKAAKEEKKKKNQSPGPGQGSEVPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTCGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGITQTYSIHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALCALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_012803709.1
Organism	Otolemur garnettii(small-eared galago)
Definition	Otolemur garnettii otoferlin (OTOF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012803709.1

ORF Insert Sequence:

ATGGCCCTGT TCGTCCACCT CAAGACAGTC TTGGAGCTGC GGGGCAAGGG CGACCGCATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TCAATGAGGC CCACACTGGC CTCAAGTCCT GGGCCACCAG CATGCTCACA 
GCCTCTCATC TCTGGTTGCT TCCTGAGGAG GTGGTGGTGG GAGGGCTAAT CGGGACCTTC 
CGAATGGTGC TGCAGAAGGT GGTGGAGGAG AGCCATGTGG AGGTGACTGA CACCCTGATT 
GATGACAATA ATGCTATCAT CAAGACCAGC CTGTGCGTGG AGGTCCGGTA TCGAGCCACA 
GATGGCACTG TGGGCTCCTG GGATGATGGG GACTTCCTGG GGGATGAGTC TCTTCAAGAG 
GAAGAGAAGG ACAGCCAGGA AACAGACGGA CTGCTGCCTG GTTCCCGTCC CAGTTCCCGG 
CCACCAGGAG AGAAGAGCTT CCGGAGCAAA GGCAGAGAGA AGACCAAGGG AGGCAGAGAT 
GGCGAGTACA AAGCCGGGAG GAGCGTGTTC TCCGCCATGA AGCTCGGTAA GAGCCGGACC 
CACAAGGAGG AGCCCCAAAG ACAAGACGAA CCAGCAGTGC TGGAGATGGA GGACCTGGAC 
CATCTGGCCA TTCGGCTAGG GGACAGGCTG GATCCTGACT CTGTGTCTCT GGCCTCAGTC 
ACAGCTCTCA CTACCAATGT CTCTAACAAG CGATCTAAGC CAGACATAAA GATGGAGCCA 
AGTGCTGGGC GGCCCATGGA TTACCAGGTC AGCATCACGG TGATTGAGGC TCGGCAGCTG 
GTGGGCTTGA ACATGGACCC CGTGGTGTGT GTGGAGGTGG GCGATGACAA GAAGTACACA 
TCCATGAAGG AGTCCACTAA CTGCCCCTAT TACAATGAGT ACTTCGTGTT CGATTTCCAT 
GTCTCTCCTG ATGTTATGTT CGACAAAATC ATCAAGATCT CGGTGATTCA CTCCAAGAAC 
CTGCTCCGCA GCGGCACCCT GGTGGGCTCC TTCAAAATGG ATGTGGGGAC CGTGTACTCC 
CAGCCAGAGC ACCAGTTCCA TCACAAGTGG GCCATCCTGT CAGACCCTGA TGACATCTCT 
GCGGGGCTAA AGGGCTATGT GAAGTGCGAC GTCGCCGTCG TGGGCAAGGG GGACAACATC 
AAGACGCCCC ACAAGGCCAA TGAGACAGAT GAGGATGACA TTGAAGGAAA TATTCTGGGC 
CCTTCCGGGA TCCCACCAGC AAGCAGGTGG GCCCGGTTCT ATGTGAAAAT TTACCGAGCA 
GAGGGGCTGC CTCGTATGAA CACAAGCCTC ATGGCCAACG TGAAGAAGGC TTTCATTGGT 
GAAAACAAGG ACCTTGTGGA CCCCTACGTG CAAGTCTTCT TTGCTGGCCA GAAGGGCAAG 
ACTTCAGTGC AGAAGAGCAG CTACGAGCCA CTGTGGAATG AGCAGGTCGT CTTCACAGAC 
CTCTTCCCTC CTCTCTGCAA ACGCATGAAG GTACAGATCC GAGACTCAGA CAAGGTCAAT 
GATGTGGCCA TCGGCACCCA CTTCATAGAC CTGCGCAAGA TTTCCAATGA TGGAGACAAA 
GGCTTCCTGC CCACACTGGG CCCTGCCTGG GTGAACATGT ATGGCTCCAC ACGCAACTAC 
ACGCTGCTGG ACGAGCACCA GGACTTGAAT GAGGGCCTGG GCGAGGGTGT GTCTTTCCGT 
GCCCGTCTCC TGCTAGGCCT GGCAGTGGAG ATCCTGGACA CCTCCAACCC TGAGCTCACC 
AGCTCCACAG AGGTACAATT GGAGCAGGCC ACACCCATCT CAGAGAGCTG CACTGGGAAA 
ATGGACGAGT TCTTTCTCTT TGGAGCCTTC CTGGAGGCTT CAATGATCGA CCGGAAAAAT 
GGAGACAAGC CAATCACCTT TGAGGTCACC ATAGGCAACT ATGGGAACGA AGTGGATGGA 
CTGTCACGAC CCCAATGGTC ACGGCCACGG AAGGAGCCCG GGGATGAGGA GGAAGTAGAC 
TTGATTCAGA ACTCCAGTGA TGATGAGGGT AGTGACAATG GGGACCTGGC CTCAGTCTCT 
TCCACACCAC CCATGCGGCC CCAGGTCACT GACAGGAACT ACTTCCATCT GCCCTACCTG 
GAGCGCAAGC CCTGCATCTA CATCAAGAGC TGGTGGCCAG ACCAGCGTCG TCGCCTCTAC 
AACGCCAATG TCATGGACCA CATTGCTGAC AAGCTGGAGG AAGGCCTGAA TGACGTGCAG 
GAGATGATCA AGACAGAGAA GTCCTACCCG GAGCGTCGCC TGCGGGGTGT CCTGGAGGAG 
CTCAGCTGTG GCTGCTACCG TTTCCTCTCC CTCGCGGACA AGGACCAGAG CCACTCCTCC 
CGTACCAGGC TTGACCGGGA GCGCCTCAAG TCCTGCATGA GGGAGCTGGA GAACATGGGA 
CACCAGGCAC GGACCCTGCG GGCCCAGGTG AAGCGGCACA CGGTGCGGGA CAAGCTGAGG 
CTGTGCCAGA ACTTCCTGCA GAAGCTGCGC TTCCTGGCAG ATGAGCCTCA GCATAGCATC 
CCCGACGTCT TCATCTGGAT GATGAGCAAC AACAAGCGCA TCGCCTACTC GCGGGTGCCC 
TCCAAGGACC TGCTCTTCTC CATCGTGGAG GAGGAGATGG GCAAGGACTG CGCCAAGGTC 
AAGACGCTCT TCCTGAAGCT GCCGGGAAAG CGAGGCTTTG GCTCAGCCGG CTGGACGGTG 
CAGGCTAAGC TGGAGCTATA TCTGTGGCTG GGCCTCAGCA AGCAGCGCAA GGAATTCCTG 
TGTGGCCTGC CTTGTGGCTT CGAGGAGGTC AAGGCGGCCC AGGGCCTGGG CCTGCACTCC 
TTCCCCCCAA TCAGCCTGGT CTACACCAAG AAGCAGGCCT TCCAGCTTCG AGCCCACATG 
TACCAGGCCC GCAGCCTGTT TGCCGCTGAC AGCAGTGGGC TGTCCGACCC CTTCGCCCGT 
GTCTTCTTCA TCAACCAGAG TCAGTGCACA GAGGTGCTAA ATGAAACCCT GTGTCCCACC 
TGGGACCAGA TGCTGGTGTT CGACAACCTG GAGCTGTATG GTGAAGCTCA CGAGTTGCGG 
GATGACCCCC CCATCATTGT CATAGAAATC TATGACCAGG ATACCATGGG CAAAGCCGAC 
TTCATGGGCC GCACCTTTGC CAAGCCTGTG GTGAAGATGG CAGACGAGGC GTACTGCCCT 
CCACGCTTCC CGCCCCAGCT CGAGTACTAC CAGATCTACC GCGGCAACGC CACGGCGGGA 
GACCTGCTGG CCGCCTTCGA GCTGCTACAG ATTGGGCCAG CAGGGAAGGC TGACCTACCA 
CCCATCGATG GCCCAGTGGA TGTGGACCGA GGGCCCATCA TGCCTGTGCC TGTGGGCATC 
CGGCCCGTGC TCAGCAAGTA CCGTGTGGAG GTACTATTCT GGGGTCTGCG GGATCTGAAG 
CGAGTGAACT TGGCTCAGGT GGACCGGCCA CGGGTAGACA TCGAGTGTGC AGGAAAGGGG 
GTACAGTCGT CCCTGATCCA CAATTACAAG AAAAACCCGA ACTTCAACAC CCTGGTCAAG 
TGGTTTGAAG TGGACCTCCC AGAGAATGAG CTCCTGCACC CACCCTTGAA CATCCGTGTG 
GTGGACTGCC GGGCCTTCGG CCGCTACACC CTGGTGGGCT CCCATGCCGT CAGCTCCCTG 
CGTCGTTTCA TCTATCGGCC CCCAGACCGC TCAGCCCCCA GCTGGAACAC CACAGGGGAG 
GTCGTGGTGA ACATGGAGCC CGAGGTGCCC ATCAAGAAAC TGGAGACCAT GGTGAAGCTG 
GACGCGACTT CTGATGCTGT TGTCAAGGTG GATGTGGCTG AGGAGGAGAA GGAGAAAAAG 
AAGAAGAAGA AGAAGGGCAC CACAGAAGAG CCAGAGGAGG AGGAGCCGGA TGAGGCCATG 
CTGGACTGGT GGTCCAAGTA TTTTGCCTCC ATTGACACCA TGAAGGAGCA ACTTCGACAA 
CAAGAGGCCT CAGGAATTGA CCTGGAAGAG AAGGAAGAGG TGGAAAACAG TGAAGGCCTG 
AAGGGGCCGA TGAAGGGCAA GGAGAAGACA AAGGCGGCCA AGGAAGAAAA GAAGAAGAAG 
AACCAGAGCC CTGGTCCTGG TCAGGGGTCT GAGGTCCCAG AGAAAAAGAA ACCCAAGATT 
GATGAGCTTA AGGTGTACCC CAAGGAGCTA GAGTCTGAGT TCGACAACTT TGAGGACTGG 
CTTCACACTT TCAACCTGCT GCGGGGCAAG ACTGGGGATG ATGAGGATGG CTCTACCGAG 
GAGGAGCGCA TCGTGGGCCG GTTCAAGGGA TCCCTCTGCG TGTACAAAGT ACCACTCCCA 
GAGGATGTAT CCCGGGAAGC TGGCTACGAC CCCACCTGTG GCATGTTCCA GGGCATCCCA 
AGCAATGACC CCATCAACGT GCTGGTCCGA GTCTATGTGG TCCGGGCCAC GGACCTGCAC 
CCCGCAGACA TCAACGGCAA AGCCGACCCC TACATCGCCA TCCGGCTAGG CAAGACTGAC 
ATCCGCGACA AGGAGAACTA CATCTCCAAG CAGCTCAACC CTGTGTTTGG AAAGTCATTT 
GACATCGAAG CCTCCTTCCC CATGGAATCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC 
CTGGTAGGTA CAGATGACCT CATTGGGGAA ACCAAGATTG ACCTAGAGAA CCGCTTCTAC 
AGCAAGCACC GAGCCACTTG TGGCATCACC CAGACCTACT CCATACATGG CTACAATATC 
TGGCGAGACC CCATGAAGCC CAGCCAGATC CTGACCCGCC TCTGCAAAGA CGGCAAAGTG 
GACGGCCCTC ACTTTGGGCC CCCTGGGAGA GTGAAGGTGG CCAACCGTGT CTTCACTGGG 
CCTTCTGAGA TCGAGGATGA GAATGGTCAG AGAAAGCCCA CAGACGAGCA CGTGGCATTG 
TGCGCCCTGA GGCACTGGGA GGACATCCCC CGTGTGGGCT GTCGACTGGT GCCAGAACAT 
GTGGAGACAA GGCCACTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG 
CTGTGGGTGG ACATGTTCCC CATGGACATG CCAGCCCCTG GGACACCTCT GGACATCTCT 
CCCCGGAAAC CCAAGAAGTA TGAGCTGCGG GTCATAGTGT GGAACACAGA TGAGGTGGTC 
CTGGAGGATG ATGATTTCTT CACTGGGGAG AAGTCCAGTG ACATTTTTGT GCGGGGGTGG 
CTGAAGGGCC AGCAGGAGGA CAAGCAAGAC ACAGATGTCC ACTACCATTC CCTTACTGGT 
GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTTGACT ACCTGGCAGC GGAGGAAAAG 
ATCGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGATG AGACTGAATA CAAGATCCCC 
GCGCGGCTCA CTCTGCAGAT CTGGGATGCT GACCACTTCT CTGCTGATGA CTTCCTGGGG 
GCCATCGAGC TGGACCTGAA CCGGTTCCCA CGGGGTGCAA AGACAGCCAA GCAGTGCACC 
ATGGAGATGG CCACCGGGGA GGTGGACGTG CCTCTGGTTT CCATCTTCAA GCAGAAGCGT 
GTCAAAGGCT GGTGGCCCCT CCTGGCTCGC AATGAGAATG ACGAGTTTGA GCTCACGGGC 
AAGGTGGAGG CTGAGTTGCA TTTACTGACA GCAGAGGAGG CAGAGAAGAA TCCAGTGGGC 
CTGGCCCGCA ATGAACCTGA CCCCCTGGAG AAACCCAACC GGCCCGACAC AGCCTTCGTC 
TGGTTCCTCA ACCCCCTGAA GTCCATCAAG TACCTGATCT GCACCCGGTA CAAGTGGCTC 
ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTCATGC TGGCCCTCTT CCTCTACAGC 
ATGCCCGGCT ACATGGTCAA GAAGCTCCTG GGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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OTOF cDNA ORF clone, Otolemur garnettii(small-eared galago)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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